net cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	net
Entrez Gene ID	45339
Full Name	CG11450 gene product from transcript CG11450-RB
Synonyms	CG11450,Dmel\CG11450,Group IId,Shout,bHLHc42,shout
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21B1-21B1\|2-0.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272860.1	NP_001259789.1	net, isoform C
NM_080081.3	NP_524820.2	net, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	net	NP_001259789.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009983	XP_319121.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Based on findings in other systems, we propose that adhesion to substrate-bound Net underlies both long- and short-range Net-Fra-dependent guidance in vivo, thereby eroding the distinction between them.
Title: Frazzled promotes growth cone attachment at the source of a Netrin gradient in the

Previous results indicated that netrin phenotype is epistatic to sli or robo phenotypes. However, it seems likely that this previously reported epistatic relationship might be due to the partial penetrance of the sli, robo, robo3 (or robo2) phenotypes.
Title: Regulation of axon guidance by slit and netrin signaling in the Drosophila ventral nerve cord.

The following net gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the net cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15221	NM_001272860.1 Latest version!	Drosophila melanogaster net, transcript variant C (net), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa15221	NM_080081.3 Latest version!	Drosophila melanogaster net, transcript variant B (net), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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1 Clone	30% OFF
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

net ( NM_001272860.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259789.1 Drosophila melanogaster net, transcript variant C (net), mRNA.

Price & Availability↑

CloneID	OFa15221
Clone ID Related Accession (Same CDS sequence)	NM_001272860.1 , NM_080081.3
Accession Version	NM_001272860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	net, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAATA CCAACACAGA AAAACTTTAC ATGCAACTAA GCGCATCGGA ATTATCAGCA 
ATTATTATGA AAGATTCACC CAATTCCAAC GACCGTGATG CGGGTTTTTG TTCCGCCAGT 
TCGGAAAGCG AAGGCGGAGA CGACTTGGTC GTCGAGCACG CGCGCAGTGG CAGTCCCGAC 
ATCAGACCAA AAGGAACAGA CTCGGCGGAC TCCAAGCCAA TAGCCCTTGT CCGCAACAAA 
CGCAAGAGTT CGGAACCCTT CAAAGTCGTA GGTTTGACGA CTCCCAACTC TAAATCCATG 
CCTGGTCCTC CGTCCAGTGC GAGTATGAAT GCAACTGGTC CGCTAAAGAA ACGCATACGA 
TACACATCTT CCGCCGATTC GGCAGTTGTC CTAACGCCAC CAGCCATAGA CTCTCCACCG 
CCCAACAGTT GCATACCTTC CACGTTGCGC CTGCAGCATG AAATAATGCC TAATCCGGCA 
CATATATATG TTCGCCATCC GGGAGTTACG ACTCTTCACA GATCGCTTGC TGCCCACCCA 
GAACAGCTGG AACCACTCGC ATTAGTAACG ACCAAAAAGC AATGTGTCGA CCAAGCGGGG 
CCTAAAATAG AGGCCTTTTC CGCATTGCTA ATTGGAAAAC AGCCATCCGC CAAAAAAACG 
TTGAAGGAAA GGACGCAAAA AGAATCAACG TCATCTTCCT TTTTAGAAGC TAGTCTTAGC 
GATGAGGATC TGAACAAGAC GGGATTGGCA CCAATTTCGC GACCTCATCA ACATCAACGC 
AATTACAAGA ATATGACTAG GGAGCGCCGC ATTGAGGCGA ACGCACGTGA ACGGACCCGT 
GTACACACCA TATCCGCCGC CTACGAAACG CTCCGCCAAG CTGTTCCAGC CTATGCCAGC 
ACTCAAAAAC TTTCCAAACT CTCCGTTCTT CGGGTAGCGT GTTCCTATAT ACTAACCTTA 
AGTAGAATGG CTGGCGAGGA TTACAGCGCC GATCAATCCG TACCGTCCAT TGCCACGTGC 
TTAGAAGCTG TAACATCAAC CATCCAAACG GAGGGAAAAG TGAAACGAAA GAAAGACGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259789.1
CDS	296..1378
Translation	MANTNTEKLYMQLSASELSAIIMKDSPNSNDRDAGFCSASSESEGGDDLVVEHARSGSPDIRPKGTDSADSKPIALVRNKRKSSEPFKVVGLTTPNSKSMPGPPSSASMNATGPLKKRIRYTSSADSAVVLTPPAIDSPPPNSCIPSTLRLQHEIMPNPAHIYVRHPGVTTLHRSLAAHPEQLEPLALVTTKKQCVDQAGPKIEAFSALLIGKQPSAKKTLKERTQKESTSSSFLEASLSDEDLNKTGLAPISRPHQHQRNYKNMTRERRIEANARERTRVHTISAAYETLRQAVPAYASTQKLSKLSVLRVACSYILTLSRMAGEDYSADQSVPSIATCLEAVTSTIQTEGKVKRKKDE

Target ORF information:

RefSeq Version	NM_001272860.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster net, transcript variant C (net), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272860.1

ORF Insert Sequence:

ATGGCGAATA CCAACACAGA AAAACTTTAC ATGCAACTAA GCGCATCGGA ATTATCAGCA 
ATTATTATGA AAGATTCACC CAATTCCAAC GACCGTGATG CGGGTTTTTG TTCCGCCAGT 
TCGGAAAGCG AAGGCGGAGA CGACTTGGTC GTCGAGCACG CGCGCAGTGG CAGTCCCGAC 
ATCAGACCAA AAGGAACAGA CTCGGCGGAC TCCAAGCCAA TAGCCCTTGT CCGCAACAAA 
CGCAAGAGTT CGGAACCCTT CAAAGTCGTA GGTTTGACGA CTCCCAACTC TAAATCCATG 
CCTGGTCCTC CGTCCAGTGC GAGTATGAAT GCAACTGGTC CGCTAAAGAA ACGCATACGA 
TACACATCTT CCGCCGATTC GGCAGTTGTC CTAACGCCAC CAGCCATAGA CTCTCCACCG 
CCCAACAGTT GCATACCTTC CACGTTGCGC CTGCAGCATG AAATAATGCC TAATCCGGCA 
CATATATATG TTCGCCATCC GGGAGTTACG ACTCTTCACA GATCGCTTGC TGCCCACCCA 
GAACAGCTGG AACCACTCGC ATTAGTAACG ACCAAAAAGC AATGTGTCGA CCAAGCGGGG 
CCTAAAATAG AGGCCTTTTC CGCATTGCTA ATTGGAAAAC AGCCATCCGC CAAAAAAACG 
TTGAAGGAAA GGACGCAAAA AGAATCAACG TCATCTTCCT TTTTAGAAGC TAGTCTTAGC 
GATGAGGATC TGAACAAGAC GGGATTGGCA CCAATTTCGC GACCTCATCA ACATCAACGC 
AATTACAAGA ATATGACTAG GGAGCGCCGC ATTGAGGCGA ACGCACGTGA ACGGACCCGT 
GTACACACCA TATCCGCCGC CTACGAAACG CTCCGCCAAG CTGTTCCAGC CTATGCCAGC 
ACTCAAAAAC TTTCCAAACT CTCCGTTCTT CGGGTAGCGT GTTCCTATAT ACTAACCTTA 
AGTAGAATGG CTGGCGAGGA TTACAGCGCC GATCAATCCG TACCGTCCAT TGCCACGTGC 
TTAGAAGCTG TAACATCAAC CATCCAAACG GAGGGAAAAG TGAAACGAAA GAAAGACGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

net ( NM_080081.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524820.2 Drosophila melanogaster net, transcript variant B (net), mRNA.

Price & Availability↑

CloneID	OFa15221
Clone ID Related Accession (Same CDS sequence)	NM_001272860.1 , NM_080081.3
Accession Version	NM_080081.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	net, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_080081.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAATA CCAACACAGA AAAACTTTAC ATGCAACTAA GCGCATCGGA ATTATCAGCA 
ATTATTATGA AAGATTCACC CAATTCCAAC GACCGTGATG CGGGTTTTTG TTCCGCCAGT 
TCGGAAAGCG AAGGCGGAGA CGACTTGGTC GTCGAGCACG CGCGCAGTGG CAGTCCCGAC 
ATCAGACCAA AAGGAACAGA CTCGGCGGAC TCCAAGCCAA TAGCCCTTGT CCGCAACAAA 
CGCAAGAGTT CGGAACCCTT CAAAGTCGTA GGTTTGACGA CTCCCAACTC TAAATCCATG 
CCTGGTCCTC CGTCCAGTGC GAGTATGAAT GCAACTGGTC CGCTAAAGAA ACGCATACGA 
TACACATCTT CCGCCGATTC GGCAGTTGTC CTAACGCCAC CAGCCATAGA CTCTCCACCG 
CCCAACAGTT GCATACCTTC CACGTTGCGC CTGCAGCATG AAATAATGCC TAATCCGGCA 
CATATATATG TTCGCCATCC GGGAGTTACG ACTCTTCACA GATCGCTTGC TGCCCACCCA 
GAACAGCTGG AACCACTCGC ATTAGTAACG ACCAAAAAGC AATGTGTCGA CCAAGCGGGG 
CCTAAAATAG AGGCCTTTTC CGCATTGCTA ATTGGAAAAC AGCCATCCGC CAAAAAAACG 
TTGAAGGAAA GGACGCAAAA AGAATCAACG TCATCTTCCT TTTTAGAAGC TAGTCTTAGC 
GATGAGGATC TGAACAAGAC GGGATTGGCA CCAATTTCGC GACCTCATCA ACATCAACGC 
AATTACAAGA ATATGACTAG GGAGCGCCGC ATTGAGGCGA ACGCACGTGA ACGGACCCGT 
GTACACACCA TATCCGCCGC CTACGAAACG CTCCGCCAAG CTGTTCCAGC CTATGCCAGC 
ACTCAAAAAC TTTCCAAACT CTCCGTTCTT CGGGTAGCGT GTTCCTATAT ACTAACCTTA 
AGTAGAATGG CTGGCGAGGA TTACAGCGCC GATCAATCCG TACCGTCCAT TGCCACGTGC 
TTAGAAGCTG TAACATCAAC CATCCAAACG GAGGGAAAAG TGAAACGAAA GAAAGACGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524820.2
CDS	296..1378
Translation	MANTNTEKLYMQLSASELSAIIMKDSPNSNDRDAGFCSASSESEGGDDLVVEHARSGSPDIRPKGTDSADSKPIALVRNKRKSSEPFKVVGLTTPNSKSMPGPPSSASMNATGPLKKRIRYTSSADSAVVLTPPAIDSPPPNSCIPSTLRLQHEIMPNPAHIYVRHPGVTTLHRSLAAHPEQLEPLALVTTKKQCVDQAGPKIEAFSALLIGKQPSAKKTLKERTQKESTSSSFLEASLSDEDLNKTGLAPISRPHQHQRNYKNMTRERRIEANARERTRVHTISAAYETLRQAVPAYASTQKLSKLSVLRVACSYILTLSRMAGEDYSADQSVPSIATCLEAVTSTIQTEGKVKRKKDE

Target ORF information:

RefSeq Version	NM_080081.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster net, transcript variant B (net), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080081.3

ORF Insert Sequence:

ATGGCGAATA CCAACACAGA AAAACTTTAC ATGCAACTAA GCGCATCGGA ATTATCAGCA 
ATTATTATGA AAGATTCACC CAATTCCAAC GACCGTGATG CGGGTTTTTG TTCCGCCAGT 
TCGGAAAGCG AAGGCGGAGA CGACTTGGTC GTCGAGCACG CGCGCAGTGG CAGTCCCGAC 
ATCAGACCAA AAGGAACAGA CTCGGCGGAC TCCAAGCCAA TAGCCCTTGT CCGCAACAAA 
CGCAAGAGTT CGGAACCCTT CAAAGTCGTA GGTTTGACGA CTCCCAACTC TAAATCCATG 
CCTGGTCCTC CGTCCAGTGC GAGTATGAAT GCAACTGGTC CGCTAAAGAA ACGCATACGA 
TACACATCTT CCGCCGATTC GGCAGTTGTC CTAACGCCAC CAGCCATAGA CTCTCCACCG 
CCCAACAGTT GCATACCTTC CACGTTGCGC CTGCAGCATG AAATAATGCC TAATCCGGCA 
CATATATATG TTCGCCATCC GGGAGTTACG ACTCTTCACA GATCGCTTGC TGCCCACCCA 
GAACAGCTGG AACCACTCGC ATTAGTAACG ACCAAAAAGC AATGTGTCGA CCAAGCGGGG 
CCTAAAATAG AGGCCTTTTC CGCATTGCTA ATTGGAAAAC AGCCATCCGC CAAAAAAACG 
TTGAAGGAAA GGACGCAAAA AGAATCAACG TCATCTTCCT TTTTAGAAGC TAGTCTTAGC 
GATGAGGATC TGAACAAGAC GGGATTGGCA CCAATTTCGC GACCTCATCA ACATCAACGC 
AATTACAAGA ATATGACTAG GGAGCGCCGC ATTGAGGCGA ACGCACGTGA ACGGACCCGT 
GTACACACCA TATCCGCCGC CTACGAAACG CTCCGCCAAG CTGTTCCAGC CTATGCCAGC 
ACTCAAAAAC TTTCCAAACT CTCCGTTCTT CGGGTAGCGT GTTCCTATAT ACTAACCTTA 
AGTAGAATGG CTGGCGAGGA TTACAGCGCC GATCAATCCG TACCGTCCAT TGCCACGTGC 
TTAGAAGCTG TAACATCAAC CATCCAAACG GAGGGAAAAG TGAAACGAAA GAAAGACGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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net cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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