rhi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	rhi
Entrez Gene ID	44879
Full Name	rhino
Synonyms	CG10683,Dmel\CG10683,HP1,HP1D,HP1d,RHI,Rhi,rno
General protein information	Preferred Names rhino Names CG10683 gene product from transcript CG10683-RA CG10683-PA heterochromatin protein 1 rhi-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54D2-54D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_080533.4	NP_536794.1	rhino

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Analysis of piRNA clusters containing remnants of I-elements shows increased expression of the piRNA( PIWI-interacting RNA)precursors and enrichment by the Heterochromatin Protein 1 homolog, Rhino, in weak R strains, which is in accordance with stronger piRNA expression by these regions
Title: Natural variation of piRNA expression affects immunity to transposable elements.

Our findings may provide critical molecular insights into the recognition of piRNA clusters by Rhi.
Title: Structural insights into Rhino-mediated germline piRNA cluster formation.

Hp1d gene product can be involved in the flamenco locus primary transcript processing.
Title: Expression of hp1 family genes and their plausible role in formation of flamenco phenotype in D. melanogaster.

Study shows that Rhi binds specifically to dual-strand clusters and that binding correlates with germline piRNA production.
Title: The HP1 homolog rhino anchors a nuclear complex that suppresses piRNA precursor splicing.

Analysis of piRNA clusters in the Drosophila ovary shows that hallmarks of RNA Polymerase II (Pol II) transcription are fundamentally different at uni-strand versus dual-strand clusters. Genome-wide, piRNA production from dual-strand clusters requires the RDC complex consisting of Rhi, Deadlock (Del), and Cuff.
Title: The rhino-deadlock-cutoff complex licenses noncanonical transcription of dual-strand piRNA clusters in Drosophila.

The following rhi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rhi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11382	NM_080533.4 Latest version!	Drosophila melanogaster rhino (rhi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rhi ( NM_080533.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_536794.1 Drosophila melanogaster rhino (rhi), mRNA.

Price & Availability↑

CloneID	OFa11382
Clone ID Related Accession (Same CDS sequence)	NM_080533.4
Accession Version	NM_080533.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rhino
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_080533.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTCGCA ACCATCAGCG ACCAAATCTT GGTCTGGTCG ATGCACCGCC TAATGATCAT 
GTCGAAGAAT ATGTAGTGGA GAAAATCTTA GGCAAACGGT TTGTTAATGG GCGTCCCCAG 
GTTCTGGTGA AGTGGAGCGG TTTTCCGAAC GAGAACAACA CCTGGGAACC ATTGGAAAAC 
GTAGGCAACT GCATGAAGCT GGTCTCCGAC TTCGAGTCGG AGGTCTTCAG GCTGCATCGG 
AAGGCCGCGG CTAAATCTGT CGGGAAATCG AAGTCAAGTC CTAGCAGCAG CGGTCCATTG 
ATCACCGAGA ATGGCCCCAG CAGCTCTAAG AAAACACAAC AACATTCCAA GTCGGTGCAG 
GCAAAAAACA CAGCTGGAAT GTCGAAGATG AATCAGAAAA AAGGAAAGAA TATTAAGAAA 
ACGGCGGGAA AAATCAAAGA TATTGAAAAT TATCCCAAAA CTCAGATGCC TAGTACTAGC 
CAAGTCTCCA CAGATTCAAC TGAAGTTTTC GACGGCAATC CATCAGCCAC AACGACGAAC 
ATGATTAAGA GTCCCAGGAT TCAAAGCCTT TTCAGTGATC TGAACTTGAT CGAACCCACT 
AAAGACAAAG ATGTGGGGGA CACATCTTTA AAAACGCCTC CCAAATCGCG CCGGCTTATT 
GAATTTCCTC AACGGGAAGA CGCACCATTG TCTTCGAAAC ATGTGTCTCC TATGCTGATT 
CGTAAAGAAT CCCAACCATT GCAGAGCTCA TGTACTGATG ATTCGGATTT GGGTGAATCC 
TCTTCCAGTA TGTCGCTTCC TACAGTATCA TCGACCAGCT CTGAGAAATC GATCAAGGTC 
ACCAAATCGG AACCTAAAAC GCTCGGCCAG ATAAAGTTTT CGTCTAGGTC ATCCGATGGT 
GGTCATGCGG CTTCATCGCT GGGCGCACCA AAAGAAGGGG ACATTGGCTT GGATTTATCT 
GGCAGTGATT CCATGGATTC CGAAGTGGAG AGCATGCGCC GCTGTCCGCG CCGCAAGCGT 
AAAAAAACTT ACCCGGATTG GAAATTCCCG GAGATGACGA AACCCTTCGG TGTGAATCGC 
GGACTGGATC TGGATAAAAT TCTCCACTGC TACCAGATGA ATGACGATCT ATTCATGTTT 
GTCACGTGGA AGGGCTGCTC GTCCATCGAT GCAGTGCATA TTAATGACAT TAAGGAAGCG 
TATCCATTGC AGATAATTAA GTACTTCGAG AGCTTGAGGA TCATTGTGCC CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536794.1
CDS	41..1297
Translation	MSRNHQRPNLGLVDAPPNDHVEEYVVEKILGKRFVNGRPQVLVKWSGFPNENNTWEPLENVGNCMKLVSDFESEVFRLHRKAAAKSVGKSKSSPSSSGPLITENGPSSSKKTQQHSKSVQAKNTAGMSKMNQKKGKNIKKTAGKIKDIENYPKTQMPSTSQVSTDSTEVFDGNPSATTTNMIKSPRIQSLFSDLNLIEPTKDKDVGDTSLKTPPKSRRLIEFPQREDAPLSSKHVSPMLIRKESQPLQSSCTDDSDLGESSSSMSLPTVSSTSSEKSIKVTKSEPKTLGQIKFSSRSSDGGHAASSLGAPKEGDIGLDLSGSDSMDSEVESMRRCPRRKRKKTYPDWKFPEMTKPFGVNRGLDLDKILHCYQMNDDLFMFVTWKGCSSIDAVHINDIKEAYPLQIIKYFESLRIIVPK

Target ORF information:

RefSeq Version	NM_080533.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rhino (rhi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080533.4

ORF Insert Sequence:

ATGTCTCGCA ACCATCAGCG ACCAAATCTT GGTCTGGTCG ATGCACCGCC TAATGATCAT 
GTCGAAGAAT ATGTAGTGGA GAAAATCTTA GGCAAACGGT TTGTTAATGG GCGTCCCCAG 
GTTCTGGTGA AGTGGAGCGG TTTTCCGAAC GAGAACAACA CCTGGGAACC ATTGGAAAAC 
GTAGGCAACT GCATGAAGCT GGTCTCCGAC TTCGAGTCGG AGGTCTTCAG GCTGCATCGG 
AAGGCCGCGG CTAAATCTGT CGGGAAATCG AAGTCAAGTC CTAGCAGCAG CGGTCCATTG 
ATCACCGAGA ATGGCCCCAG CAGCTCTAAG AAAACACAAC AACATTCCAA GTCGGTGCAG 
GCAAAAAACA CAGCTGGAAT GTCGAAGATG AATCAGAAAA AAGGAAAGAA TATTAAGAAA 
ACGGCGGGAA AAATCAAAGA TATTGAAAAT TATCCCAAAA CTCAGATGCC TAGTACTAGC 
CAAGTCTCCA CAGATTCAAC TGAAGTTTTC GACGGCAATC CATCAGCCAC AACGACGAAC 
ATGATTAAGA GTCCCAGGAT TCAAAGCCTT TTCAGTGATC TGAACTTGAT CGAACCCACT 
AAAGACAAAG ATGTGGGGGA CACATCTTTA AAAACGCCTC CCAAATCGCG CCGGCTTATT 
GAATTTCCTC AACGGGAAGA CGCACCATTG TCTTCGAAAC ATGTGTCTCC TATGCTGATT 
CGTAAAGAAT CCCAACCATT GCAGAGCTCA TGTACTGATG ATTCGGATTT GGGTGAATCC 
TCTTCCAGTA TGTCGCTTCC TACAGTATCA TCGACCAGCT CTGAGAAATC GATCAAGGTC 
ACCAAATCGG AACCTAAAAC GCTCGGCCAG ATAAAGTTTT CGTCTAGGTC ATCCGATGGT 
GGTCATGCGG CTTCATCGCT GGGCGCACCA AAAGAAGGGG ACATTGGCTT GGATTTATCT 
GGCAGTGATT CCATGGATTC CGAAGTGGAG AGCATGCGCC GCTGTCCGCG CCGCAAGCGT 
AAAAAAACTT ACCCGGATTG GAAATTCCCG GAGATGACGA AACCCTTCGG TGTGAATCGC 
GGACTGGATC TGGATAAAAT TCTCCACTGC TACCAGATGA ATGACGATCT ATTCATGTTT 
GTCACGTGGA AGGGCTGCTC GTCCATCGAT GCAGTGCATA TTAATGACAT TAAGGAAGCG 
TATCCATTGC AGATAATTAA GTACTTCGAG AGCTTGAGGA TCATTGTGCC CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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rhi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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