robo2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	robo2
Entrez Gene ID	44522
Full Name	roundabout 2
Synonyms	CG14347,CG14348,CG5481,CG5574,CT17326,D-Robo2,D-robo2,Dmel\CG5481,ROBO2,Robo 2,Robo-2,Robo2,Robo2 (Lea),anon-EST:Liang-1.75,clone 1.75,dRobo-2,fus4,lea,robo-2
General protein information	Preferred Names roundabout 2 Names CG5481 gene product from transcript CG5481-RA CG5481-PA CG5481-PB Robo2 Roundabout2 SGP cluster fusion defects 4 leak robo2-PA robo2-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 22A1-22A1\|2-3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_080531.4	NP_536792.2	roundabout 2, isoform A
NM_001272939.2	NP_001259868.1	roundabout 2, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

repulsive Slit-Robo2-Ena signaling exerts an extrusive force downstream of JNK to eliminate scrib cells from epithelia by disrupting E-cadherin.
Title: Slit-Robo Repulsive Signaling Extrudes Tumorigenic Cells from Epithelia.

a Robo2-mediated mechanism, independent of signaling, is essential to limiting Slit distribution and which might be relevant to the regulation of Slit-mediated short-range signaling in additional systems
Title: A non-signaling role of Robo2 in tendons is essential for Slit processing and muscle patterning.

The authors describe a distinct mechanism to inhibit Robo1 repulsion and promote midline crossing, in which Roundabout2 (Robo2) binds to and prevents Robo1 signaling.
Title: Robo2 acts in trans to inhibit Slit-Robo1 repulsion in pre-crossing commissural axons.

Data identify Robo2 and Robo3 as key effectors of Hb9 in regulating nervous system development.
Title: The homeodomain transcription factor Hb9 controls axon guidance in Drosophila through the regulation of Robo receptors.

Data show that Robo and Robo2 promote midline repulsion, while Robo2 and Robo3 specify the position of longitudinal axon pathways.
Title: Slit/Robo-mediated axon guidance in Tribolium and Drosophila: divergent genetic programs build insect nervous systems.

The following robo2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the robo2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15208	NM_001272939.2 Latest version!	Drosophila melanogaster roundabout 2 (robo2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa16433	NM_080531.4 Latest version!	Drosophila melanogaster roundabout 2 (robo2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

robo2 ( NM_001272939.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259868.1 Drosophila melanogaster roundabout 2 (robo2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15208
Clone ID Related Accession (Same CDS sequence)	NM_001272939.2
Accession Version	NM_001272939.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	roundabout 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001272939.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTTGT GCGAGATGCG TGGAATGCCG CTGCGGTTGC AACCTCAGTG TTTAACCAAC 
GAAAGCGAAA TTCAAATTAA AATCAAAAGC AAAATCAAAA ATAAAACTGC CAGCGAGATC 
AAACGCCGAC ACAGAAACAA TCGAGATTTT CATGGGGAGC GGCTAAAAAT AATGCCAGTC 
GATCGGAGTC GACGCGCAGT ATTCCCACTG CTGCTGCTCC TCGCGGGATT AAACGGATTA 
ACTCAAGTCG GAGCGCTCAA GGGTGAAAAT CCACGCATCA TCGAGCATCC CATGGACACG 
ACGGTGCCAA AAAATGATCC ATTTACGTTT AATTGCCAGG CCGAGGGCAA TCCAACACCA 
ACCATTCAAT GGTTTAAGGA CGGTCGCGAA CTGAAGACGG ATACGGGTTC GCATCGCATA 
ATGCTGCCCG CCGGGGGTCT ATTCTTTCTC AAGGTTATCC ACTCACGTAG AGAGAGCGAT 
GCGGGCACTT ACTGGTGCGA GGCCAAAAAC GAGTTTGGAG TGGCACGGTC CAGGAATGCA 
ACGTTGCAAG TGGCATTTCT CCGCGACGAA TTCCGTTTGG AGCCGGCAAA TACCCGCGTG 
GCCCAAGGCG AGGTGGCCCT GATGGAATGC GGTGCCCCCC GAGGATCTCC GGAGCCGCAA 
ATCTCGTGGC GCAAGAACGG CCAGACCCTG AATCTTGTCG GGAACAAGCG GATTCGCATT 
GTCGACGGTG GCAATCTGGC CATCCAGGAA GCCCGCCAAT CGGACGACGG ACGCTACCAG 
TGTGTGGTCA AGAATGTGGT TGGCACCCGG GAGTCGGCCA CCGCTTTTCT TAAAGTGCAT 
GTACGTCCAT TCCTCATCCG AGGACCCCAG AATCAGACGG CGGTGGTGGG CAGCTCGGTG 
GTCTTCCAGT GCCGCATCGG AGGCGATCCC CTGCCTGATG TCCTGTGGCG ACGCACTGCC 
TCCGGCGGCA ATATGCCACT GCGTCGTGTG CACGTACTTG AGGACCGCAG TCTGAAGCTG 
GACGACGTTA CTCTGGAGGA CATGGGCGAG TACACTTGCG AGGCGGACAA TGCGGTGGGC 
GGCATCACGG CCACTGGCAT CCTCACCGTT CACGCTCCCC CCAAATTTGT GATACGCCCC 
AAGAATCAGC TGGTGGAGAT CGGTGATGAA GTGCTGTTCG AGTGCCAAGC GAATGGACAT 
CCCCGACCAA CGCTCTACTG GTCGGTGGAG GGCAACAGCT CCCTGCTGCT CCCCGGCTAT 
CGGGATGGCC GCATGGAAGT GACCCTGACG CCCGAGGGGC GCTCGGTGCT CTCGATAGCT 
CGATTTGCCC GTGAGGATTC CGGAAAGGTG GTCACTTGCA ACGCCCTGAA CGCCGTGGGC 
AGCGTCAGCA GTCGGACTGT GGTCAGTGTG GATACGCAAT TCGAGCTGCC ACCGCCGATT 
ATCGAACAGG GGCCCGTGAA TCAAACGTTG CCCGTTAAAT CAATTGTGGT TCTGCCATGC 
CGAACTCTGG GCACTCCAGT GCCACAGGTC TCTTGGTACC TGGATGGCAT ACCCATCGAT 
GTGCAGGAGC ACGAGCGGCG GAATCTTTCG GACGCTGGAG CCTTAACCAT TTCGGATCTT 
CAGCGCCACG AGGATGAAGG CTTGTACACC TGCGTGGCCA GCAATCGCAA CGGAAAATCC 
TCTTGGAGTG GTTACCTTCG TCTGGACACC CCGACAAATC CGAATATCAA GTTCTTCAGA 
GCCCCAGAAC TTTCCACCTA CCCAGGGCCG CCAGGAAAAC CGCAAATGGT GGAGAAGGGC 
GAAAATTCGG TGACTCTCAG CTGGACGAGG AGCAACAAGG TGGGCGGCTC CAGTCTGGTG 
GGCTATGTAA TCGAGATGTT TGGCAAAAAC GAAACGGATG GCTGGGTGGC TGTGGGCACT 
AGGGTGCAAA ATACCACGTT TACCCAAACG GGTCTGCTGC CGGGTGTGAA TTACTTCTTT 
CTAATTCGAG CCGAGAACTC CCATGGCTTA TCACTGCCCA GTCCGATGTC GGAACCCATT 
ACGGTGGGAA CGCGCTACTT CAATAGTGGT CTGGATCTGA GCGAGGCTCG TGCCAGTCTG 
CTGTCCGGAG ATGTTGTGGA GCTGAGCAAC GCCAGTGTGG TGGACTCCAC TAGCATGAAA 
CTCACCTGGC AGATCATCAA TGGCAAATAC GTCGAGGGCT TCTATGTCTA TGCGAGACAG 
TTGCCAAATC CAATAGTCAA CAATCCGGCG CCCGTTACTA GCAATACCAA TCCGCTGCTG 
GGCTCTACAT CCACATCCGC ATCCGCATCC GCCTCGGCAT CGGCATTGAT TTCGACAAAG 
CCAAATATTG CAGCTGCCGG CAAACGTGAT GGGGAGACAA ACCAGAGTGG AGGAGGAGCT 
CCGACCCCAC TGAACACCAA GTATCGCATG CTAACGATTC TCAATGGCGG TGGCGCCTCA 
TCCTGCACCA TCACCGGGCT CGTCCAGTAC ACGCTGTATG AATTTTTCAT CGTGCCATTT 
TACAAATCCG TCGAGGGCAA GCCGTCGAAT TCGCGCATCG CTCGCACCCT TGAAGATGTT 
CCCTCTGAGG CACCATATGG AATGGAGGCT CTGCTGTTGA ACTCCTCCGC GGTCTTCCTC 
AAATGGAAGG CACCAGAACT CAAGGATCGG CATGGTGTTC TCTTGAACTA TCATGTTATA 
GTCCGAGGTA TTGACACTGC CCACAATTTC TCACGCATTT TGACAAATGT CACCATCGAT 
GCCGCTTCGC CTACTCTGGT TTTGGCCAAT CTCACCGAAG GCGTCATGTA CACCGTGGGC 
GTGGCGGCCG GAAATAACGC TGGAGTTGGT CCTTATTGTG TCCCAGCTAC TTTGCGTTTG 
GATCCCATCA CAAAGCGACT CGATCCGTTC ATCAATCAGC GCTATCCCAT CAATCAGGAC 
CATGTTAACG ATGTGCTGAC GCAGCCCTGG TTCATAATAC TCCTGGGCGC CATCCTGGCC 
GTTCTTATGC TGTCCTTTGG CGCAATGGTC TTTGTGAAGC GCAAGCACAT GATGATGAAG 
CAGTCGGCCC TAAATACAAT GCGTGGCAAT CACACGAGCG ACGTGCTCAA AATGCCGAGT 
CTATCGGCGC GCAATGGAAA CGGCTACTGG CTGGACTCCT CCACCGGCGG AATGGTGTGG 
CGTCCCTCGC CCGGCGGCGA CTCGCTGGAG ATGCAAAAGG ATCACATCGC CGACTATGCG 
CCGGTCTGCG GTGCCCCCGG TTCTCCGGCC GGCGGTGGCA CCTCTTCCGG TGGATCCGGT 
GGCGCGGGCA GCGGTGCCAG CGGCGGCGAT GACATTCATG GAGGACACGG CAGCGAACGC 
AATCAGCAGC GGTACGTGGG CGAGTACTCC AACATACCGA CCGACTATGC AGAGGTGTCC 
AGTTTTGGCA AGGCACCCAG CGAGTATGGT CGGCATGGCA ACGCCTCCCC GGCCCCTTAT 
GCCACCTCTT CGATCCTGAG TCCCCACCAG CAGCAACAGC AGCAGCAGCC GCGTTATCAA 
CAGCGACCAG TGCCCGGCTA TGGGCTCCAG CGCCCAATGC ACCCACACTA CCAGCAGCAG 
CAGCATCAGC AGCAACAGGC GCAGCAGACG CACCAGCAAC ACCAGGCTCT CCAGCAGCAC 
CAGCAACTGC CACCCAGCAA CATCTACCAG CAGATGTCCA CCACCAGCGA GATATACCCC 
ACGAACACGG GTCCTTCGCG CTCTGTCTAC TCTGAGCAGT ATTACTACCC CAAGGACAAG 
CAGAGACACA TCCACATCAC CGAGAACAAG CTGAGCAACT GCCACACCTA TGAGGCGGCT 
CCTGGCGCCA AGCAGTCCTC GCCGATATCC TCGCAGTTCG CCAGCGTGAG GCGGCAGCAG 
CTGCCGCCCA ACTGCAGCAT CGGCAGGGAA AGTGCCCGCT TCAAGGTGCT AAACACGGAT 
CAGGGCAAGA ACCAGCAGAA TCTCCTGGAT CTCGACGGCT CCTCGATGTG CTACAACGGT 
CTGGCAGACT CGGGCTGCGG TGGATCTCCC TCCCCGATGG CCATGCTGAT GTCGCACGAG 
GACGAGCACG CGCTGTACCA CACGGCGGAT GGGGATCTGG ACGACATGGA ACGACTGTAC 
GTCAAGGTGG ACGAGCAGCA GCCTCCACAG CAGCAGCAGC AGCTGATTCC CCTGGTCCCA 
CAGCATCCGG CGGAAGGTCA CCTGCAGTCC TGGCGGAATC AGAGCACGCG GAGCAGTCGG 
AAGAACGGCC AGGAATGCAT CAAGGAACCC AGCGAGTTGA TCTACGCTCC GGGAAGCGTG 
GCCAGCGAAC GGAGCCTCCT CAGCAACTCG GGTAGCGGCA CCAGCAGCCA GCCAGCTGGC 
CACAATGTCT GACCTTTGCT CCCTGGTTCA TCCTTGGGCC AGTCTCAGTC CAAATCCCAG 
TCGCAGTCGC AGTCCCAGAG TGATCTGCAG TCCCAGGAAC AACTCTACGC TCGGGAAGTG 
CGGGAATTAC TCGCCTGGTG CGAACGCTTC AACAACGGTG GAGTGGAGAA CTTTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259868.1
CDS	289..4848
Translation	MHLCEMRGMPLRLQPQCLTNESEIQIKIKSKIKNKTASEIKRRHRNNRDFHGERLKIMPVDRSRRAVFPLLLLLAGLNGLTQVGALKGENPRIIEHPMDTTVPKNDPFTFNCQAEGNPTPTIQWFKDGRELKTDTGSHRIMLPAGGLFFLKVIHSRRESDAGTYWCEAKNEFGVARSRNATLQVAFLRDEFRLEPANTRVAQGEVALMECGAPRGSPEPQISWRKNGQTLNLVGNKRIRIVDGGNLAIQEARQSDDGRYQCVVKNVVGTRESATAFLKVHVRPFLIRGPQNQTAVVGSSVVFQCRIGGDPLPDVLWRRTASGGNMPLRRVHVLEDRSLKLDDVTLEDMGEYTCEADNAVGGITATGILTVHAPPKFVIRPKNQLVEIGDEVLFECQANGHPRPTLYWSVEGNSSLLLPGYRDGRMEVTLTPEGRSVLSIARFAREDSGKVVTCNALNAVGSVSSRTVVSVDTQFELPPPIIEQGPVNQTLPVKSIVVLPCRTLGTPVPQVSWYLDGIPIDVQEHERRNLSDAGALTISDLQRHEDEGLYTCVASNRNGKSSWSGYLRLDTPTNPNIKFFRAPELSTYPGPPGKPQMVEKGENSVTLSWTRSNKVGGSSLVGYVIEMFGKNETDGWVAVGTRVQNTTFTQTGLLPGVNYFFLIRAENSHGLSLPSPMSEPITVGTRYFNSGLDLSEARASLLSGDVVELSNASVVDSTSMKLTWQIINGKYVEGFYVYARQLPNPIVNNPAPVTSNTNPLLGSTSTSASASASASALISTKPNIAAAGKRDGETNQSGGGAPTPLNTKYRMLTILNGGGASSCTITGLVQYTLYEFFIVPFYKSVEGKPSNSRIARTLEDVPSEAPYGMEALLLNSSAVFLKWKAPELKDRHGVLLNYHVIVRGIDTAHNFSRILTNVTIDAASPTLVLANLTEGVMYTVGVAAGNNAGVGPYCVPATLRLDPITKRLDPFINQRYPINQDHVNDVLTQPWFIILLGAILAVLMLSFGAMVFVKRKHMMMKQSALNTMRGNHTSDVLKMPSLSARNGNGYWLDSSTGGMVWRPSPGGDSLEMQKDHIADYAPVCGAPGSPAGGGTSSGGSGGAGSGASGGDDIHGGHGSERNQQRYVGEYSNIPTDYAEVSSFGKAPSEYGRHGNASPAPYATSSILSPHQQQQQQQPRYQQRPVPGYGLQRPMHPHYQQQQHQQQQAQQTHQQHQALQQHQQLPPSNIYQQMSTTSEIYPTNTGPSRSVYSEQYYYPKDKQRHIHITENKLSNCHTYEAAPGAKQSSPISSQFASVRRQQLPPNCSIGRESARFKVLNTDQGKNQQNLLDLDGSSMCYNGLADSGCGGSPSPMAMLMSHEDEHALYHTADGDLDDMERLYVKVDEQQPPQQQQQLIPLVPQHPAEGHLQSWRNQSTRSSRKNGQECIKEPSELIYAPGSVASERSLLSNSGSGTSSQPAGHNV*PLLPGSSLGQSQSKSQSQSQSQSDLQSQEQLYAREVRELLAWCERFNNGGVENFE

Target ORF information:

RefSeq Version	NM_001272939.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster roundabout 2 (robo2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272939.2

ORF Insert Sequence:

ATGCATTTGT GCGAGATGCG TGGAATGCCG CTGCGGTTGC AACCTCAGTG TTTAACCAAC 
GAAAGCGAAA TTCAAATTAA AATCAAAAGC AAAATCAAAA ATAAAACTGC CAGCGAGATC 
AAACGCCGAC ACAGAAACAA TCGAGATTTT CATGGGGAGC GGCTAAAAAT AATGCCAGTC 
GATCGGAGTC GACGCGCAGT ATTCCCACTG CTGCTGCTCC TCGCGGGATT AAACGGATTA 
ACTCAAGTCG GAGCGCTCAA GGGTGAAAAT CCACGCATCA TCGAGCATCC CATGGACACG 
ACGGTGCCAA AAAATGATCC ATTTACGTTT AATTGCCAGG CCGAGGGCAA TCCAACACCA 
ACCATTCAAT GGTTTAAGGA CGGTCGCGAA CTGAAGACGG ATACGGGTTC GCATCGCATA 
ATGCTGCCCG CCGGGGGTCT ATTCTTTCTC AAGGTTATCC ACTCACGTAG AGAGAGCGAT 
GCGGGCACTT ACTGGTGCGA GGCCAAAAAC GAGTTTGGAG TGGCACGGTC CAGGAATGCA 
ACGTTGCAAG TGGCATTTCT CCGCGACGAA TTCCGTTTGG AGCCGGCAAA TACCCGCGTG 
GCCCAAGGCG AGGTGGCCCT GATGGAATGC GGTGCCCCCC GAGGATCTCC GGAGCCGCAA 
ATCTCGTGGC GCAAGAACGG CCAGACCCTG AATCTTGTCG GGAACAAGCG GATTCGCATT 
GTCGACGGTG GCAATCTGGC CATCCAGGAA GCCCGCCAAT CGGACGACGG ACGCTACCAG 
TGTGTGGTCA AGAATGTGGT TGGCACCCGG GAGTCGGCCA CCGCTTTTCT TAAAGTGCAT 
GTACGTCCAT TCCTCATCCG AGGACCCCAG AATCAGACGG CGGTGGTGGG CAGCTCGGTG 
GTCTTCCAGT GCCGCATCGG AGGCGATCCC CTGCCTGATG TCCTGTGGCG ACGCACTGCC 
TCCGGCGGCA ATATGCCACT GCGTCGTGTG CACGTACTTG AGGACCGCAG TCTGAAGCTG 
GACGACGTTA CTCTGGAGGA CATGGGCGAG TACACTTGCG AGGCGGACAA TGCGGTGGGC 
GGCATCACGG CCACTGGCAT CCTCACCGTT CACGCTCCCC CCAAATTTGT GATACGCCCC 
AAGAATCAGC TGGTGGAGAT CGGTGATGAA GTGCTGTTCG AGTGCCAAGC GAATGGACAT 
CCCCGACCAA CGCTCTACTG GTCGGTGGAG GGCAACAGCT CCCTGCTGCT CCCCGGCTAT 
CGGGATGGCC GCATGGAAGT GACCCTGACG CCCGAGGGGC GCTCGGTGCT CTCGATAGCT 
CGATTTGCCC GTGAGGATTC CGGAAAGGTG GTCACTTGCA ACGCCCTGAA CGCCGTGGGC 
AGCGTCAGCA GTCGGACTGT GGTCAGTGTG GATACGCAAT TCGAGCTGCC ACCGCCGATT 
ATCGAACAGG GGCCCGTGAA TCAAACGTTG CCCGTTAAAT CAATTGTGGT TCTGCCATGC 
CGAACTCTGG GCACTCCAGT GCCACAGGTC TCTTGGTACC TGGATGGCAT ACCCATCGAT 
GTGCAGGAGC ACGAGCGGCG GAATCTTTCG GACGCTGGAG CCTTAACCAT TTCGGATCTT 
CAGCGCCACG AGGATGAAGG CTTGTACACC TGCGTGGCCA GCAATCGCAA CGGAAAATCC 
TCTTGGAGTG GTTACCTTCG TCTGGACACC CCGACAAATC CGAATATCAA GTTCTTCAGA 
GCCCCAGAAC TTTCCACCTA CCCAGGGCCG CCAGGAAAAC CGCAAATGGT GGAGAAGGGC 
GAAAATTCGG TGACTCTCAG CTGGACGAGG AGCAACAAGG TGGGCGGCTC CAGTCTGGTG 
GGCTATGTAA TCGAGATGTT TGGCAAAAAC GAAACGGATG GCTGGGTGGC TGTGGGCACT 
AGGGTGCAAA ATACCACGTT TACCCAAACG GGTCTGCTGC CGGGTGTGAA TTACTTCTTT 
CTAATTCGAG CCGAGAACTC CCATGGCTTA TCACTGCCCA GTCCGATGTC GGAACCCATT 
ACGGTGGGAA CGCGCTACTT CAATAGTGGT CTGGATCTGA GCGAGGCTCG TGCCAGTCTG 
CTGTCCGGAG ATGTTGTGGA GCTGAGCAAC GCCAGTGTGG TGGACTCCAC TAGCATGAAA 
CTCACCTGGC AGATCATCAA TGGCAAATAC GTCGAGGGCT TCTATGTCTA TGCGAGACAG 
TTGCCAAATC CAATAGTCAA CAATCCGGCG CCCGTTACTA GCAATACCAA TCCGCTGCTG 
GGCTCTACAT CCACATCCGC ATCCGCATCC GCCTCGGCAT CGGCATTGAT TTCGACAAAG 
CCAAATATTG CAGCTGCCGG CAAACGTGAT GGGGAGACAA ACCAGAGTGG AGGAGGAGCT 
CCGACCCCAC TGAACACCAA GTATCGCATG CTAACGATTC TCAATGGCGG TGGCGCCTCA 
TCCTGCACCA TCACCGGGCT CGTCCAGTAC ACGCTGTATG AATTTTTCAT CGTGCCATTT 
TACAAATCCG TCGAGGGCAA GCCGTCGAAT TCGCGCATCG CTCGCACCCT TGAAGATGTT 
CCCTCTGAGG CACCATATGG AATGGAGGCT CTGCTGTTGA ACTCCTCCGC GGTCTTCCTC 
AAATGGAAGG CACCAGAACT CAAGGATCGG CATGGTGTTC TCTTGAACTA TCATGTTATA 
GTCCGAGGTA TTGACACTGC CCACAATTTC TCACGCATTT TGACAAATGT CACCATCGAT 
GCCGCTTCGC CTACTCTGGT TTTGGCCAAT CTCACCGAAG GCGTCATGTA CACCGTGGGC 
GTGGCGGCCG GAAATAACGC TGGAGTTGGT CCTTATTGTG TCCCAGCTAC TTTGCGTTTG 
GATCCCATCA CAAAGCGACT CGATCCGTTC ATCAATCAGC GCTATCCCAT CAATCAGGAC 
CATGTTAACG ATGTGCTGAC GCAGCCCTGG TTCATAATAC TCCTGGGCGC CATCCTGGCC 
GTTCTTATGC TGTCCTTTGG CGCAATGGTC TTTGTGAAGC GCAAGCACAT GATGATGAAG 
CAGTCGGCCC TAAATACAAT GCGTGGCAAT CACACGAGCG ACGTGCTCAA AATGCCGAGT 
CTATCGGCGC GCAATGGAAA CGGCTACTGG CTGGACTCCT CCACCGGCGG AATGGTGTGG 
CGTCCCTCGC CCGGCGGCGA CTCGCTGGAG ATGCAAAAGG ATCACATCGC CGACTATGCG 
CCGGTCTGCG GTGCCCCCGG TTCTCCGGCC GGCGGTGGCA CCTCTTCCGG TGGATCCGGT 
GGCGCGGGCA GCGGTGCCAG CGGCGGCGAT GACATTCATG GAGGACACGG CAGCGAACGC 
AATCAGCAGC GGTACGTGGG CGAGTACTCC AACATACCGA CCGACTATGC AGAGGTGTCC 
AGTTTTGGCA AGGCACCCAG CGAGTATGGT CGGCATGGCA ACGCCTCCCC GGCCCCTTAT 
GCCACCTCTT CGATCCTGAG TCCCCACCAG CAGCAACAGC AGCAGCAGCC GCGTTATCAA 
CAGCGACCAG TGCCCGGCTA TGGGCTCCAG CGCCCAATGC ACCCACACTA CCAGCAGCAG 
CAGCATCAGC AGCAACAGGC GCAGCAGACG CACCAGCAAC ACCAGGCTCT CCAGCAGCAC 
CAGCAACTGC CACCCAGCAA CATCTACCAG CAGATGTCCA CCACCAGCGA GATATACCCC 
ACGAACACGG GTCCTTCGCG CTCTGTCTAC TCTGAGCAGT ATTACTACCC CAAGGACAAG 
CAGAGACACA TCCACATCAC CGAGAACAAG CTGAGCAACT GCCACACCTA TGAGGCGGCT 
CCTGGCGCCA AGCAGTCCTC GCCGATATCC TCGCAGTTCG CCAGCGTGAG GCGGCAGCAG 
CTGCCGCCCA ACTGCAGCAT CGGCAGGGAA AGTGCCCGCT TCAAGGTGCT AAACACGGAT 
CAGGGCAAGA ACCAGCAGAA TCTCCTGGAT CTCGACGGCT CCTCGATGTG CTACAACGGT 
CTGGCAGACT CGGGCTGCGG TGGATCTCCC TCCCCGATGG CCATGCTGAT GTCGCACGAG 
GACGAGCACG CGCTGTACCA CACGGCGGAT GGGGATCTGG ACGACATGGA ACGACTGTAC 
GTCAAGGTGG ACGAGCAGCA GCCTCCACAG CAGCAGCAGC AGCTGATTCC CCTGGTCCCA 
CAGCATCCGG CGGAAGGTCA CCTGCAGTCC TGGCGGAATC AGAGCACGCG GAGCAGTCGG 
AAGAACGGCC AGGAATGCAT CAAGGAACCC AGCGAGTTGA TCTACGCTCC GGGAAGCGTG 
GCCAGCGAAC GGAGCCTCCT CAGCAACTCG GGTAGCGGCA CCAGCAGCCA GCCAGCTGGC 
CACAATGTCT GACCTTTGCT CCCTGGTTCA TCCTTGGGCC AGTCTCAGTC CAAATCCCAG 
TCGCAGTCGC AGTCCCAGAG TGATCTGCAG TCCCAGGAAC AACTCTACGC TCGGGAAGTG 
CGGGAATTAC TCGCCTGGTG CGAACGCTTC AACAACGGTG GAGTGGAGAA CTTTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( NM_080531.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_536792.2 Drosophila melanogaster roundabout 2 (robo2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16433
Clone ID Related Accession (Same CDS sequence)	NM_080531.4
Accession Version	NM_080531.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	roundabout 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_080531.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTTGT GCGAGATGCG TGGAATGCCG CTGCGGTTGC AACCTCAGTG TTTAACCAAC 
GAAAGCGAAA TTCAAATTAA AATCAAAAGC AAAATCAAAA ATAAAACTGC CAGCGAGATC 
AAACGCCGAC ACAGAAACAA TCGAGATTTT CATGGGGAGC GGCTAAAAAT AATGCCAGTC 
GATCGGAGTC GACGCGCAGT ATTCCCACTG CTGCTGCTCC TCGCGGGATT AAACGGATTA 
ACTCAAGTCG GAGCGCTCAA GGGTGAAAAT CCACGCATCA TCGAGCATCC CATGGACACG 
ACGGTGCCAA AAAATGATCC ATTTACGTTT AATTGCCAGG CCGAGGGCAA TCCAACACCA 
ACCATTCAAT GGTTTAAGGA CGGTCGCGAA CTGAAGACGG ATACGGGTTC GCATCGCATA 
ATGCTGCCCG CCGGGGGTCT ATTCTTTCTC AAGGTTATCC ACTCACGTAG AGAGAGCGAT 
GCGGGCACTT ACTGGTGCGA GGCCAAAAAC GAGTTTGGAG TGGCACGGTC CAGGAATGCA 
ACGTTGCAAG TGGCATTTCT CCGCGACGAA TTCCGTTTGG AGCCGGCAAA TACCCGCGTG 
GCCCAAGGCG AGGTGGCCCT GATGGAATGC GGTGCCCCCC GAGGATCTCC GGAGCCGCAA 
ATCTCGTGGC GCAAGAACGG CCAGACCCTG AATCTTGTCG GGAACAAGCG GATTCGCATT 
GTCGACGGTG GCAATCTGGC CATCCAGGAA GCCCGCCAAT CGGACGACGG ACGCTACCAG 
TGTGTGGTCA AGAATGTGGT TGGCACCCGG GAGTCGGCCA CCGCTTTTCT TAAAGTGCAT 
GTACGTCCAT TCCTCATCCG AGGACCCCAG AATCAGACGG CGGTGGTGGG CAGCTCGGTG 
GTCTTCCAGT GCCGCATCGG AGGCGATCCC CTGCCTGATG TCCTGTGGCG ACGCACTGCC 
TCCGGCGGCA ATATGCCACT GCGTCGTGTG CACGTACTTG AGGACCGCAG TCTGAAGCTG 
GACGACGTTA CTCTGGAGGA CATGGGCGAG TACACTTGCG AGGCGGACAA TGCGGTGGGC 
GGCATCACGG CCACTGGCAT CCTCACCGTT CACGCTCCCC CCAAATTTGT GATACGCCCC 
AAGAATCAGC TGGTGGAGAT CGGTGATGAA GTGCTGTTCG AGTGCCAAGC GAATGGACAT 
CCCCGACCAA CGCTCTACTG GTCGGTGGAG GGCAACAGCT CCCTGCTGCT CCCCGGCTAT 
CGGGATGGCC GCATGGAAGT GACCCTGACG CCCGAGGGGC GCTCGGTGCT CTCGATAGCT 
CGATTTGCCC GTGAGGATTC CGGAAAGGTG GTCACTTGCA ACGCCCTGAA CGCCGTGGGC 
AGCGTCAGCA GTCGGACTGT GGTCAGTGTG GATACGCAAT TCGAGCTGCC ACCGCCGATT 
ATCGAACAGG GGCCCGTGAA TCAAACGTTG CCCGTTAAAT CAATTGTGGT TCTGCCATGC 
CGAACTCTGG GCACTCCAGT GCCACAGGTC TCTTGGTACC TGGATGGCAT ACCCATCGAT 
GTGCAGGAGC ACGAGCGGCG GAATCTTTCG GACGCTGGAG CCTTAACCAT TTCGGATCTT 
CAGCGCCACG AGGATGAAGG CTTGTACACC TGCGTGGCCA GCAATCGCAA CGGAAAATCC 
TCTTGGAGTG GTTACCTTCG TCTGGACACC CCGACAAATC CGAATATCAA GTTCTTCAGA 
GCCCCAGAAC TTTCCACCTA CCCAGGGCCG CCAGGAAAAC CGCAAATGGT GGAGAAGGGC 
GAAAATTCGG TGACTCTCAG CTGGACGAGG AGCAACAAGG TGGGCGGCTC CAGTCTGGTG 
GGCTATGTAA TCGAGATGTT TGGCAAAAAC GAAACGGATG GCTGGGTGGC TGTGGGCACT 
AGGGTGCAAA ATACCACGTT TACCCAAACG GGTCTGCTGC CGGGTGTGAA TTACTTCTTT 
CTAATTCGAG CCGAGAACTC CCATGGCTTA TCACTGCCCA GTCCGATGTC GGAACCCATT 
ACGGTGGGAA CGCGCTACTT CAATAGTGGT CTGGATCTGA GCGAGGCTCG TGCCAGTCTG 
CTGTCCGGAG ATGTTGTGGA GCTGAGCAAC GCCAGTGTGG TGGACTCCAC TAGCATGAAA 
CTCACCTGGC AGATCATCAA TGGCAAATAC GTCGAGGGCT TCTATGTCTA TGCGAGACAG 
TTGCCAAATC CAATAGTCAA CAATCCGGCG CCCGTTACTA GCAATACCAA TCCGCTGCTG 
GGCTCTACAT CCACATCCGC ATCCGCATCC GCCTCGGCAT CGGCATTGAT TTCGACAAAG 
CCAAATATTG CAGCTGCCGG CAAACGTGAT GGGGAGACAA ACCAGAGTGG AGGAGGAGCT 
CCGACCCCAC TGAACACCAA GTATCGCATG CTAACGATTC TCAATGGCGG TGGCGCCTCA 
TCCTGCACCA TCACCGGGCT CGTCCAGTAC ACGCTGTATG AATTTTTCAT CGTGCCATTT 
TACAAATCCG TCGAGGGCAA GCCGTCGAAT TCGCGCATCG CTCGCACCCT TGAAGATGTT 
CCCTCTGAGG CACCATATGG AATGGAGGCT CTGCTGTTGA ACTCCTCCGC GGTCTTCCTC 
AAATGGAAGG CACCAGAACT CAAGGATCGG CATGGTGTTC TCTTGAACTA TCATGTTATA 
GTCCGAGGTA TTGACACTGC CCACAATTTC TCACGCATTT TGACAAATGT CACCATCGAT 
GCCGCTTCGC CTACTCTGGT TTTGGCCAAT CTCACCGAAG GCGTCATGTA CACCGTGGGC 
GTGGCGGCCG GAAATAACGC TGGAGTTGGT CCTTATTGTG TCCCAGCTAC TTTGCGTTTG 
GATCCCATCA CAAAGCGACT CGATCCGTTC ATCAATCAGC GCTATCCCAT CAATCAGGAC 
CATGTTAACG ATGTGCTGAC GCAGCCCTGG TTCATAATAC TCCTGGGCGC CATCCTGGCC 
GTTCTTATGC TGTCCTTTGG CGCAATGGTC TTTGTGAAGC GCAAGCACAT GATGATGAAG 
CAGTCGGCCC TAAATACAAT GCGTGGCAAT CACACGAGCG ACGTGCTCAA AATGCCGAGT 
CTATCGGCGC GCAATGGAAA CGGCTACTGG CTGGACTCCT CCACCGGCGG AATGGTGTGG 
CGTCCCTCGC CCGGCGGCGA CTCGCTGGAG ATGCAAAAGG ATCACATCGC CGACTATGCG 
CCGGTCTGCG GTGCCCCCGG TTCTCCGGCC GGCGGTGGCA CCTCTTCCGG TGGATCCGGT 
GGCGCGGGCA GCGGTGCCAG CGGCGGCGAT GACATTCATG GAGGACACGG CAGCGAACGC 
AATCAGCAGC GGTACGTGGG CGAGTACTCC AACATACCGA CCGACTATGC AGAGGTGTCC 
AGTTTTGGCA AGGCACCCAG CGAGTATGGT CGGCATGGCA ACGCCTCCCC GGCCCCTTAT 
GCCACCTCTT CGATCCTGAG TCCCCACCAG CAGCAACAGC AGCAGCAGCC GCGTTATCAA 
CAGCGACCAG TGCCCGGCTA TGGGCTCCAG CGCCCAATGC ACCCACACTA CCAGCAGCAG 
CAGCATCAGC AGCAACAGGC GCAGCAGACG CACCAGCAAC ACCAGGCTCT CCAGCAGCAC 
CAGCAACTGC CACCCAGCAA CATCTACCAG CAGATGTCCA CCACCAGCGA GATATACCCC 
ACGAACACGG GTCCTTCGCG CTCTGTCTAC TCTGAGCAGT ATTACTACCC CAAGGACAAG 
CAGAGACACA TCCACATCAC CGAGAACAAG CTGAGCAACT GCCACACCTA TGAGGCGGCT 
CCTGGCGCCA AGCAGTCCTC GCCGATATCC TCGCAGTTCG CCAGCGTGAG GCGGCAGCAG 
CTGCCGCCCA ACTGCAGCAT CGGCAGGGAA AGTGCCCGCT TCAAGGTGCT AAACACGGAT 
CAGGGCAAGA ACCAGCAGAA TCTCCTGGAT CTCGACGGCT CCTCGATGTG CTACAACGGT 
CTGGCAGACT CGGGCTGCGG TGGATCTCCC TCCCCGATGG CCATGCTGAT GTCGCACGAG 
GACGAGCACG CGCTGTACCA CACGGCGGAT GGGGATCTGG ACGACATGGA ACGACTGTAC 
GTCAAGGTGG ACGAGCAGCA GCCTCCACAG CAGCAGCAGC AGCTGATTCC CCTGGTCCCA 
CAGCATCCGG CGGAAGGTCA CCTGCAGTCC TGGCGGAATC AGAGCACGCG GAGCAGTCGG 
AAGAACGGCC AGGAATGCAT CAAGGAACCC AGCGAGTTGA TCTACGCTCC GGGAAGCGTG 
GCCAGCGAAC GGAGCCTCCT CAGCAACTCG GGTAGCGGCA CCAGCAGCCA GCCAGCTGGC 
CACAATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536792.2
CDS	289..4680
Translation	MHLCEMRGMPLRLQPQCLTNESEIQIKIKSKIKNKTASEIKRRHRNNRDFHGERLKIMPVDRSRRAVFPLLLLLAGLNGLTQVGALKGENPRIIEHPMDTTVPKNDPFTFNCQAEGNPTPTIQWFKDGRELKTDTGSHRIMLPAGGLFFLKVIHSRRESDAGTYWCEAKNEFGVARSRNATLQVAFLRDEFRLEPANTRVAQGEVALMECGAPRGSPEPQISWRKNGQTLNLVGNKRIRIVDGGNLAIQEARQSDDGRYQCVVKNVVGTRESATAFLKVHVRPFLIRGPQNQTAVVGSSVVFQCRIGGDPLPDVLWRRTASGGNMPLRRVHVLEDRSLKLDDVTLEDMGEYTCEADNAVGGITATGILTVHAPPKFVIRPKNQLVEIGDEVLFECQANGHPRPTLYWSVEGNSSLLLPGYRDGRMEVTLTPEGRSVLSIARFAREDSGKVVTCNALNAVGSVSSRTVVSVDTQFELPPPIIEQGPVNQTLPVKSIVVLPCRTLGTPVPQVSWYLDGIPIDVQEHERRNLSDAGALTISDLQRHEDEGLYTCVASNRNGKSSWSGYLRLDTPTNPNIKFFRAPELSTYPGPPGKPQMVEKGENSVTLSWTRSNKVGGSSLVGYVIEMFGKNETDGWVAVGTRVQNTTFTQTGLLPGVNYFFLIRAENSHGLSLPSPMSEPITVGTRYFNSGLDLSEARASLLSGDVVELSNASVVDSTSMKLTWQIINGKYVEGFYVYARQLPNPIVNNPAPVTSNTNPLLGSTSTSASASASASALISTKPNIAAAGKRDGETNQSGGGAPTPLNTKYRMLTILNGGGASSCTITGLVQYTLYEFFIVPFYKSVEGKPSNSRIARTLEDVPSEAPYGMEALLLNSSAVFLKWKAPELKDRHGVLLNYHVIVRGIDTAHNFSRILTNVTIDAASPTLVLANLTEGVMYTVGVAAGNNAGVGPYCVPATLRLDPITKRLDPFINQRYPINQDHVNDVLTQPWFIILLGAILAVLMLSFGAMVFVKRKHMMMKQSALNTMRGNHTSDVLKMPSLSARNGNGYWLDSSTGGMVWRPSPGGDSLEMQKDHIADYAPVCGAPGSPAGGGTSSGGSGGAGSGASGGDDIHGGHGSERNQQRYVGEYSNIPTDYAEVSSFGKAPSEYGRHGNASPAPYATSSILSPHQQQQQQQPRYQQRPVPGYGLQRPMHPHYQQQQHQQQQAQQTHQQHQALQQHQQLPPSNIYQQMSTTSEIYPTNTGPSRSVYSEQYYYPKDKQRHIHITENKLSNCHTYEAAPGAKQSSPISSQFASVRRQQLPPNCSIGRESARFKVLNTDQGKNQQNLLDLDGSSMCYNGLADSGCGGSPSPMAMLMSHEDEHALYHTADGDLDDMERLYVKVDEQQPPQQQQQLIPLVPQHPAEGHLQSWRNQSTRSSRKNGQECIKEPSELIYAPGSVASERSLLSNSGSGTSSQPAGHNV

Target ORF information:

RefSeq Version	NM_080531.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster roundabout 2 (robo2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080531.4

ORF Insert Sequence:

ATGCATTTGT GCGAGATGCG TGGAATGCCG CTGCGGTTGC AACCTCAGTG TTTAACCAAC 
GAAAGCGAAA TTCAAATTAA AATCAAAAGC AAAATCAAAA ATAAAACTGC CAGCGAGATC 
AAACGCCGAC ACAGAAACAA TCGAGATTTT CATGGGGAGC GGCTAAAAAT AATGCCAGTC 
GATCGGAGTC GACGCGCAGT ATTCCCACTG CTGCTGCTCC TCGCGGGATT AAACGGATTA 
ACTCAAGTCG GAGCGCTCAA GGGTGAAAAT CCACGCATCA TCGAGCATCC CATGGACACG 
ACGGTGCCAA AAAATGATCC ATTTACGTTT AATTGCCAGG CCGAGGGCAA TCCAACACCA 
ACCATTCAAT GGTTTAAGGA CGGTCGCGAA CTGAAGACGG ATACGGGTTC GCATCGCATA 
ATGCTGCCCG CCGGGGGTCT ATTCTTTCTC AAGGTTATCC ACTCACGTAG AGAGAGCGAT 
GCGGGCACTT ACTGGTGCGA GGCCAAAAAC GAGTTTGGAG TGGCACGGTC CAGGAATGCA 
ACGTTGCAAG TGGCATTTCT CCGCGACGAA TTCCGTTTGG AGCCGGCAAA TACCCGCGTG 
GCCCAAGGCG AGGTGGCCCT GATGGAATGC GGTGCCCCCC GAGGATCTCC GGAGCCGCAA 
ATCTCGTGGC GCAAGAACGG CCAGACCCTG AATCTTGTCG GGAACAAGCG GATTCGCATT 
GTCGACGGTG GCAATCTGGC CATCCAGGAA GCCCGCCAAT CGGACGACGG ACGCTACCAG 
TGTGTGGTCA AGAATGTGGT TGGCACCCGG GAGTCGGCCA CCGCTTTTCT TAAAGTGCAT 
GTACGTCCAT TCCTCATCCG AGGACCCCAG AATCAGACGG CGGTGGTGGG CAGCTCGGTG 
GTCTTCCAGT GCCGCATCGG AGGCGATCCC CTGCCTGATG TCCTGTGGCG ACGCACTGCC 
TCCGGCGGCA ATATGCCACT GCGTCGTGTG CACGTACTTG AGGACCGCAG TCTGAAGCTG 
GACGACGTTA CTCTGGAGGA CATGGGCGAG TACACTTGCG AGGCGGACAA TGCGGTGGGC 
GGCATCACGG CCACTGGCAT CCTCACCGTT CACGCTCCCC CCAAATTTGT GATACGCCCC 
AAGAATCAGC TGGTGGAGAT CGGTGATGAA GTGCTGTTCG AGTGCCAAGC GAATGGACAT 
CCCCGACCAA CGCTCTACTG GTCGGTGGAG GGCAACAGCT CCCTGCTGCT CCCCGGCTAT 
CGGGATGGCC GCATGGAAGT GACCCTGACG CCCGAGGGGC GCTCGGTGCT CTCGATAGCT 
CGATTTGCCC GTGAGGATTC CGGAAAGGTG GTCACTTGCA ACGCCCTGAA CGCCGTGGGC 
AGCGTCAGCA GTCGGACTGT GGTCAGTGTG GATACGCAAT TCGAGCTGCC ACCGCCGATT 
ATCGAACAGG GGCCCGTGAA TCAAACGTTG CCCGTTAAAT CAATTGTGGT TCTGCCATGC 
CGAACTCTGG GCACTCCAGT GCCACAGGTC TCTTGGTACC TGGATGGCAT ACCCATCGAT 
GTGCAGGAGC ACGAGCGGCG GAATCTTTCG GACGCTGGAG CCTTAACCAT TTCGGATCTT 
CAGCGCCACG AGGATGAAGG CTTGTACACC TGCGTGGCCA GCAATCGCAA CGGAAAATCC 
TCTTGGAGTG GTTACCTTCG TCTGGACACC CCGACAAATC CGAATATCAA GTTCTTCAGA 
GCCCCAGAAC TTTCCACCTA CCCAGGGCCG CCAGGAAAAC CGCAAATGGT GGAGAAGGGC 
GAAAATTCGG TGACTCTCAG CTGGACGAGG AGCAACAAGG TGGGCGGCTC CAGTCTGGTG 
GGCTATGTAA TCGAGATGTT TGGCAAAAAC GAAACGGATG GCTGGGTGGC TGTGGGCACT 
AGGGTGCAAA ATACCACGTT TACCCAAACG GGTCTGCTGC CGGGTGTGAA TTACTTCTTT 
CTAATTCGAG CCGAGAACTC CCATGGCTTA TCACTGCCCA GTCCGATGTC GGAACCCATT 
ACGGTGGGAA CGCGCTACTT CAATAGTGGT CTGGATCTGA GCGAGGCTCG TGCCAGTCTG 
CTGTCCGGAG ATGTTGTGGA GCTGAGCAAC GCCAGTGTGG TGGACTCCAC TAGCATGAAA 
CTCACCTGGC AGATCATCAA TGGCAAATAC GTCGAGGGCT TCTATGTCTA TGCGAGACAG 
TTGCCAAATC CAATAGTCAA CAATCCGGCG CCCGTTACTA GCAATACCAA TCCGCTGCTG 
GGCTCTACAT CCACATCCGC ATCCGCATCC GCCTCGGCAT CGGCATTGAT TTCGACAAAG 
CCAAATATTG CAGCTGCCGG CAAACGTGAT GGGGAGACAA ACCAGAGTGG AGGAGGAGCT 
CCGACCCCAC TGAACACCAA GTATCGCATG CTAACGATTC TCAATGGCGG TGGCGCCTCA 
TCCTGCACCA TCACCGGGCT CGTCCAGTAC ACGCTGTATG AATTTTTCAT CGTGCCATTT 
TACAAATCCG TCGAGGGCAA GCCGTCGAAT TCGCGCATCG CTCGCACCCT TGAAGATGTT 
CCCTCTGAGG CACCATATGG AATGGAGGCT CTGCTGTTGA ACTCCTCCGC GGTCTTCCTC 
AAATGGAAGG CACCAGAACT CAAGGATCGG CATGGTGTTC TCTTGAACTA TCATGTTATA 
GTCCGAGGTA TTGACACTGC CCACAATTTC TCACGCATTT TGACAAATGT CACCATCGAT 
GCCGCTTCGC CTACTCTGGT TTTGGCCAAT CTCACCGAAG GCGTCATGTA CACCGTGGGC 
GTGGCGGCCG GAAATAACGC TGGAGTTGGT CCTTATTGTG TCCCAGCTAC TTTGCGTTTG 
GATCCCATCA CAAAGCGACT CGATCCGTTC ATCAATCAGC GCTATCCCAT CAATCAGGAC 
CATGTTAACG ATGTGCTGAC GCAGCCCTGG TTCATAATAC TCCTGGGCGC CATCCTGGCC 
GTTCTTATGC TGTCCTTTGG CGCAATGGTC TTTGTGAAGC GCAAGCACAT GATGATGAAG 
CAGTCGGCCC TAAATACAAT GCGTGGCAAT CACACGAGCG ACGTGCTCAA AATGCCGAGT 
CTATCGGCGC GCAATGGAAA CGGCTACTGG CTGGACTCCT CCACCGGCGG AATGGTGTGG 
CGTCCCTCGC CCGGCGGCGA CTCGCTGGAG ATGCAAAAGG ATCACATCGC CGACTATGCG 
CCGGTCTGCG GTGCCCCCGG TTCTCCGGCC GGCGGTGGCA CCTCTTCCGG TGGATCCGGT 
GGCGCGGGCA GCGGTGCCAG CGGCGGCGAT GACATTCATG GAGGACACGG CAGCGAACGC 
AATCAGCAGC GGTACGTGGG CGAGTACTCC AACATACCGA CCGACTATGC AGAGGTGTCC 
AGTTTTGGCA AGGCACCCAG CGAGTATGGT CGGCATGGCA ACGCCTCCCC GGCCCCTTAT 
GCCACCTCTT CGATCCTGAG TCCCCACCAG CAGCAACAGC AGCAGCAGCC GCGTTATCAA 
CAGCGACCAG TGCCCGGCTA TGGGCTCCAG CGCCCAATGC ACCCACACTA CCAGCAGCAG 
CAGCATCAGC AGCAACAGGC GCAGCAGACG CACCAGCAAC ACCAGGCTCT CCAGCAGCAC 
CAGCAACTGC CACCCAGCAA CATCTACCAG CAGATGTCCA CCACCAGCGA GATATACCCC 
ACGAACACGG GTCCTTCGCG CTCTGTCTAC TCTGAGCAGT ATTACTACCC CAAGGACAAG 
CAGAGACACA TCCACATCAC CGAGAACAAG CTGAGCAACT GCCACACCTA TGAGGCGGCT 
CCTGGCGCCA AGCAGTCCTC GCCGATATCC TCGCAGTTCG CCAGCGTGAG GCGGCAGCAG 
CTGCCGCCCA ACTGCAGCAT CGGCAGGGAA AGTGCCCGCT TCAAGGTGCT AAACACGGAT 
CAGGGCAAGA ACCAGCAGAA TCTCCTGGAT CTCGACGGCT CCTCGATGTG CTACAACGGT 
CTGGCAGACT CGGGCTGCGG TGGATCTCCC TCCCCGATGG CCATGCTGAT GTCGCACGAG 
GACGAGCACG CGCTGTACCA CACGGCGGAT GGGGATCTGG ACGACATGGA ACGACTGTAC 
GTCAAGGTGG ACGAGCAGCA GCCTCCACAG CAGCAGCAGC AGCTGATTCC CCTGGTCCCA 
CAGCATCCGG CGGAAGGTCA CCTGCAGTCC TGGCGGAATC AGAGCACGCG GAGCAGTCGG 
AAGAACGGCC AGGAATGCAT CAAGGAACCC AGCGAGTTGA TCTACGCTCC GGGAAGCGTG 
GCCAGCGAAC GGAGCCTCCT CAGCAACTCG GGTAGCGGCA CCAGCAGCCA GCCAGCTGGC 
CACAATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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robo2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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