tio cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tio
Entrez Gene ID	44272
Full Name	tiptop
Synonyms	CG12630,Dmel\CG12630,Tio
General protein information	Preferred Names tiptop Names CG12630 gene product from transcript CG12630-RB CG12630-PA CG12630-PB tio-PA tio-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 40D3-40D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_079994.4	NP_524733.2	tiptop, isoform A
NM_001273750.1	NP_001260679.1	tiptop, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009028	XP_319778.4
Drosophila melanogaster (fruit fly)	tio	NP_001260679.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tiptop/teashirt genes regulate cell differentiation and renal physiology in Drosophila.
Title: The tiptop/teashirt genes regulate cell differentiation and renal physiology in Drosophila.

Tio has four such Zinc Finger domains Tio bind to C-terminal Binding Protein (CtBP) and that this interaction is important for promoting both cell proliferation and eye development.
Title: A dissection of the teashirt and tiptop genes reveals a novel mechanism for regulating transcription factor activity.

Data show that tiptop appears to be a more effective inducer of retinal formation than teashirt.
Title: Restriction of ectopic eye formation by Drosophila teashirt and tiptop to the developing antenna.

Data show that tio (tiptop) and tsh (teashirt) have coincident expression domains in the imaginal discs, the precursors of the adult body, and that both genes show similar functional properties when expressed ectopically.
Title: Zinc-finger paralogues tsh and tio are functionally equivalent during imaginal development in Drosophila and maintain their expression levels through auto- and cross-negative feedback loops.

Teashirt and Tiptop repress each other's expression
Title: A critical role of teashirt for patterning the ventral epidermis is masked by ectopic expression of tiptop, a paralog of teashirt in Drosophila.

The following tio gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tio cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15199	NM_079994.4 Latest version!	Drosophila melanogaster tiptop (tio), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa15199	NM_001273750.1 Latest version!	Drosophila melanogaster tiptop (tio), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tio ( NM_079994.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524733.2 Drosophila melanogaster tiptop (tio), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15199
Clone ID Related Accession (Same CDS sequence)	NM_079994.4 , NM_001273750.1
Accession Version	NM_079994.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tiptop, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_079994.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCTGC ACGAGGCCGT CATGCTGGAA ATCTATCGCC AGGCGCTGAG TGCAAGTGAG 
TTAACTAGTC CGCGATGTCA GTCTCGCGAT TCCAACACCT CAGCTGGAGC GGGTGCTGGA 
ATGGCCGATG TCCGATGTCC ATCGAATGAG TCGCATTGTT CGGCGAACGA CCGTCTGACC 
CCAGCTGCAA CGCCCACTCT CACGCCTACT GAAGCAACCA TCTCCCCAAA CTCGGTGGGA 
CTTCCCCTGA CGGCGACCCT TCCTCCTGCA GCTGCAGTTG CTCTGCTACC GCCCCAATCC 
GCCGCCATGG CCGCTTACCT AGCCGCTGCC CAACAAAATC ACCTTCTGCT AACAAATCCA 
TTGGCGGCAG CAGCTTCACT GGTCCAACAT GCAACGCAGC AGGCAGTGGT GGAGGGGGAG 
GTGGAGTCGC CGGCCCTCGA TTTTAGCAGA AAGCGGCCTA AGAGCCACGG AGACGACGAT 
CAGGAAGAAG ATCAGGAACA GGACCAGGAA CAGGAGCAGG AGCAGGAGCC CGATCATGAT 
GTCCAGTGCG ATAATGGTCC TTTAGATCTA TCTGTAAGCA CAGGGAAAAG GCAAGAATCG 
GTTTCTCCGC CTGCCAGGAA GATTCCAAGA AGTATATCCG CAGATTACAA GTCACCTCTT 
CCACCTGGCA GCTGGATGCC CCCCATCAAC CCATACTTGG CAGCTGTAGC GGCAAAGACG 
GGCGGACTGG GATATTCTAA GTTAGCACCC AGTGAAGCCA GCAAAGCGTT GGAGAAGATG 
ACTGAGATGA GCCGATTGGA AACATCCCCA ACAGCTGCCC GAAGCTTAGG CGCCACATCA 
TCTGTAGGAG CAGGAGTGCC TGCGGGAGCT AGTTCCAACT CCGGTGGCCG GCACAGCGCG 
TGGCAGTCTC ATTGGCTGAA CAAAGGTGCT GATACTGCAA AGGATGTGTT TAAGTGTGTG 
TGGTGCAAGC AGAGCTTTTC AACTCTGGCT AACTTAACTG CCCACATGAA GGAGACTCAG 
CATTGCGGTG TGCAGATACC CTCGCCGTTG CCAACCGGAG GAGTCGGCAC TCCGTCTGCT 
CCTCCTCCCA CAAGGTTGGC CACTTCAGCC TCCAACTCGG CCTGTTCCAG CAGCAGTAGC 
TCCACCTCCA GCTCTTCAAA CTCCTCGAAA TCAGAGTTAA ATATGCTAAT TAAGGAAACT 
ATGCCCCTTC CCAGGAAATT GGTGAGGGGT CAGGATGTTT GGCTCGGAAA GGGTGCGGAG 
CAGACCCGCC AGATACTTAA GTGCATGTGG TGTGGACAAA GCTTCCGGTC TCTGGCCGAG 
ATGACCAGCC ACATGCAGGA GACGCAGCAC TACACGAATA TCATCTCGCA GGAGCAGATC 
ATTTCCTGGA AGTCGGGCGA TGAACGGGAG AGGCCGACCA ACACTGGAGT TCCGTCTACG 
TCCACCGCTG CTCCCTCTTC GCCGAGTTGC ACAGCTCCAT CAGTGAGTGC CGTGCTCACG 
TGTAAAGTTT GTGATCAGGC CTTCGGTAGC CTAAAGGAGT TAAGCACCCA TATGGCCCAA 
AAATCGCACT ACAAGGAGTC ACCGGCTCCG TCAGCATCTC CTCCAGCTGC CGGCACTGGC 
AATCCGAAAA GAGGAAGGCA AAATCGCAAC GAGAAACGCA AGAAATCCCT GCCGGTTAGG 
AAGCTTCTTG AGCTCGAGCG TTCCGGCTCG AATTCAAGTC TGGACTCAGC GCTAAAGCCC 
TTGAGGGACT TTGCTGCTGC CACCAAAATT ACCTGTGAAA AATGTGGCAG TAAGATTGAA 
ACTGCGTTGT TTGTCGAGCA CATACGAAAG TGCCTGGGCG AGAGTATTCC AATCCCTCCC 
CGTCGATCTA ACGCGGGAGT AGATCGCCTG CCAAGTCCCA GTCTAGGACT TGGCGCTGAA 
AAGCCACCAT CAGTCCTTAA TGCCCTGGAG CAACTTATAG AGAAGAGCTT CGAATCGAGA 
ACCAGCAGAA CCATGACACA TGGAGGCTAT TCCGAAGCTG GAACTCCGCT TGGAGCCAGC 
ATCCTAAAGC GTTTGGGAAT TGAGGACAGC AGTGACTACA CCAAGCCCCT TATGGATGCC 
CAGGCGATGC ACCTGCTCCG ATCCTCCTTC GCCTCGAGAG ACCGCAGTGC CAGCGAATCG 
AGCTCAGCCA GTAGGGTGGA ATCATCCTAC ACCCCAGACA GACAGCAGGC CACCCCCCAC 
AAAAGTCCAG ATACTCCTGC TCCGCCTCCT CCCCCACCGC CAACCATCAA AGCTGAACCA 
CTAGAGGCGG AACCCTTGGT GGGTTGCGAT CGGGAGGGCT GCAGTCCTCG GCAGCAGATC 
CAAGTGAAAA AGGAGTTCAG CATGGAAGCA TGTCGGGAGA GTCCTCGGTC TGTAAGCAAA 
TCACCTGCGC CGCAGACCGA AAGATCTCCA CCAGATAATG GCAGTCTACT GGCGTTGAAC 
TCCATGTTCG ATCAGCTCAG TGGAGTGGAG AACAGCGGCA ATAACAACTC GGGGCATTGC 
TTTAACAACA ACAACAGCTG CAGTAGCGTC TCCGCCCAGA AGCCAAAGGC CCATCCTCTA 
GCTGCGCTGC AGAAACTGTG CGAGACCACG GATCCCCCAA GCACTGGCCT GCGCAGCGCG 
TCCTCCGCCG GATCCTCCAC AGCATCAGCA ACACTTCCCT CAGCGAACGG TAACGACCTG 
GTGGCCTTCA GCTGGGCCTG CAACGAGGCA GTTCTGAGTG CCAGTAATGG TGGCTCCGCC 
GGCGATTCGT CCATCATTAA GTGCTCCTAC TGTGATACCC CTTTTGCATC GAAGGGCGCC 
TACCGCCACC ATCTCTCCAA GGTGCACTTT GTGAAAGACG CAGGCGAGGA CTCGCCCAGG 
CTCAAGTCCC CCGCCGTTCA GAGCCCCAGG TCGATGCCAT TGGCCTCCCC AAGGAGATCA 
GCTTCCAGGA GTCCGGCGAC AGGATCTCAG CAGCCCCCTC CCTCGCCAAC CATTAGCCCC 
TATGACGAGA GTCCGCAGTC AAAGTTCCTC AAATACACGG AGCTGGCCAA ACAGCTATCC 
TCTAAAAACG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524733.2
CDS	714..3788
Translation	MMLHEAVMLEIYRQALSASELTSPRCQSRDSNTSAGAGAGMADVRCPSNESHCSANDRLTPAATPTLTPTEATISPNSVGLPLTATLPPAAAVALLPPQSAAMAAYLAAAQQNHLLLTNPLAAAASLVQHATQQAVVEGEVESPALDFSRKRPKSHGDDDQEEDQEQDQEQEQEQEPDHDVQCDNGPLDLSVSTGKRQESVSPPARKIPRSISADYKSPLPPGSWMPPINPYLAAVAAKTGGLGYSKLAPSEASKALEKMTEMSRLETSPTAARSLGATSSVGAGVPAGASSNSGGRHSAWQSHWLNKGADTAKDVFKCVWCKQSFSTLANLTAHMKETQHCGVQIPSPLPTGGVGTPSAPPPTRLATSASNSACSSSSSSTSSSSNSSKSELNMLIKETMPLPRKLVRGQDVWLGKGAEQTRQILKCMWCGQSFRSLAEMTSHMQETQHYTNIISQEQIISWKSGDERERPTNTGVPSTSTAAPSSPSCTAPSVSAVLTCKVCDQAFGSLKELSTHMAQKSHYKESPAPSASPPAAGTGNPKRGRQNRNEKRKKSLPVRKLLELERSGSNSSLDSALKPLRDFAAATKITCEKCGSKIETALFVEHIRKCLGESIPIPPRRSNAGVDRLPSPSLGLGAEKPPSVLNALEQLIEKSFESRTSRTMTHGGYSEAGTPLGASILKRLGIEDSSDYTKPLMDAQAMHLLRSSFASRDRSASESSSASRVESSYTPDRQQATPHKSPDTPAPPPPPPPTIKAEPLEAEPLVGCDREGCSPRQQIQVKKEFSMEACRESPRSVSKSPAPQTERSPPDNGSLLALNSMFDQLSGVENSGNNNSGHCFNNNNSCSSVSAQKPKAHPLAALQKLCETTDPPSTGLRSASSAGSSTASATLPSANGNDLVAFSWACNEAVLSASNGGSAGDSSIIKCSYCDTPFASKGAYRHHLSKVHFVKDAGEDSPRLKSPAVQSPRSMPLASPRRSASRSPATGSQQPPPSPTISPYDESPQSKFLKYTELAKQLSSKNA

Target ORF information:

RefSeq Version	NM_079994.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tiptop (tio), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079994.4

ORF Insert Sequence:

ATGATGCTGC ACGAGGCCGT CATGCTGGAA ATCTATCGCC AGGCGCTGAG TGCAAGTGAG 
TTAACTAGTC CGCGATGTCA GTCTCGCGAT TCCAACACCT CAGCTGGAGC GGGTGCTGGA 
ATGGCCGATG TCCGATGTCC ATCGAATGAG TCGCATTGTT CGGCGAACGA CCGTCTGACC 
CCAGCTGCAA CGCCCACTCT CACGCCTACT GAAGCAACCA TCTCCCCAAA CTCGGTGGGA 
CTTCCCCTGA CGGCGACCCT TCCTCCTGCA GCTGCAGTTG CTCTGCTACC GCCCCAATCC 
GCCGCCATGG CCGCTTACCT AGCCGCTGCC CAACAAAATC ACCTTCTGCT AACAAATCCA 
TTGGCGGCAG CAGCTTCACT GGTCCAACAT GCAACGCAGC AGGCAGTGGT GGAGGGGGAG 
GTGGAGTCGC CGGCCCTCGA TTTTAGCAGA AAGCGGCCTA AGAGCCACGG AGACGACGAT 
CAGGAAGAAG ATCAGGAACA GGACCAGGAA CAGGAGCAGG AGCAGGAGCC CGATCATGAT 
GTCCAGTGCG ATAATGGTCC TTTAGATCTA TCTGTAAGCA CAGGGAAAAG GCAAGAATCG 
GTTTCTCCGC CTGCCAGGAA GATTCCAAGA AGTATATCCG CAGATTACAA GTCACCTCTT 
CCACCTGGCA GCTGGATGCC CCCCATCAAC CCATACTTGG CAGCTGTAGC GGCAAAGACG 
GGCGGACTGG GATATTCTAA GTTAGCACCC AGTGAAGCCA GCAAAGCGTT GGAGAAGATG 
ACTGAGATGA GCCGATTGGA AACATCCCCA ACAGCTGCCC GAAGCTTAGG CGCCACATCA 
TCTGTAGGAG CAGGAGTGCC TGCGGGAGCT AGTTCCAACT CCGGTGGCCG GCACAGCGCG 
TGGCAGTCTC ATTGGCTGAA CAAAGGTGCT GATACTGCAA AGGATGTGTT TAAGTGTGTG 
TGGTGCAAGC AGAGCTTTTC AACTCTGGCT AACTTAACTG CCCACATGAA GGAGACTCAG 
CATTGCGGTG TGCAGATACC CTCGCCGTTG CCAACCGGAG GAGTCGGCAC TCCGTCTGCT 
CCTCCTCCCA CAAGGTTGGC CACTTCAGCC TCCAACTCGG CCTGTTCCAG CAGCAGTAGC 
TCCACCTCCA GCTCTTCAAA CTCCTCGAAA TCAGAGTTAA ATATGCTAAT TAAGGAAACT 
ATGCCCCTTC CCAGGAAATT GGTGAGGGGT CAGGATGTTT GGCTCGGAAA GGGTGCGGAG 
CAGACCCGCC AGATACTTAA GTGCATGTGG TGTGGACAAA GCTTCCGGTC TCTGGCCGAG 
ATGACCAGCC ACATGCAGGA GACGCAGCAC TACACGAATA TCATCTCGCA GGAGCAGATC 
ATTTCCTGGA AGTCGGGCGA TGAACGGGAG AGGCCGACCA ACACTGGAGT TCCGTCTACG 
TCCACCGCTG CTCCCTCTTC GCCGAGTTGC ACAGCTCCAT CAGTGAGTGC CGTGCTCACG 
TGTAAAGTTT GTGATCAGGC CTTCGGTAGC CTAAAGGAGT TAAGCACCCA TATGGCCCAA 
AAATCGCACT ACAAGGAGTC ACCGGCTCCG TCAGCATCTC CTCCAGCTGC CGGCACTGGC 
AATCCGAAAA GAGGAAGGCA AAATCGCAAC GAGAAACGCA AGAAATCCCT GCCGGTTAGG 
AAGCTTCTTG AGCTCGAGCG TTCCGGCTCG AATTCAAGTC TGGACTCAGC GCTAAAGCCC 
TTGAGGGACT TTGCTGCTGC CACCAAAATT ACCTGTGAAA AATGTGGCAG TAAGATTGAA 
ACTGCGTTGT TTGTCGAGCA CATACGAAAG TGCCTGGGCG AGAGTATTCC AATCCCTCCC 
CGTCGATCTA ACGCGGGAGT AGATCGCCTG CCAAGTCCCA GTCTAGGACT TGGCGCTGAA 
AAGCCACCAT CAGTCCTTAA TGCCCTGGAG CAACTTATAG AGAAGAGCTT CGAATCGAGA 
ACCAGCAGAA CCATGACACA TGGAGGCTAT TCCGAAGCTG GAACTCCGCT TGGAGCCAGC 
ATCCTAAAGC GTTTGGGAAT TGAGGACAGC AGTGACTACA CCAAGCCCCT TATGGATGCC 
CAGGCGATGC ACCTGCTCCG ATCCTCCTTC GCCTCGAGAG ACCGCAGTGC CAGCGAATCG 
AGCTCAGCCA GTAGGGTGGA ATCATCCTAC ACCCCAGACA GACAGCAGGC CACCCCCCAC 
AAAAGTCCAG ATACTCCTGC TCCGCCTCCT CCCCCACCGC CAACCATCAA AGCTGAACCA 
CTAGAGGCGG AACCCTTGGT GGGTTGCGAT CGGGAGGGCT GCAGTCCTCG GCAGCAGATC 
CAAGTGAAAA AGGAGTTCAG CATGGAAGCA TGTCGGGAGA GTCCTCGGTC TGTAAGCAAA 
TCACCTGCGC CGCAGACCGA AAGATCTCCA CCAGATAATG GCAGTCTACT GGCGTTGAAC 
TCCATGTTCG ATCAGCTCAG TGGAGTGGAG AACAGCGGCA ATAACAACTC GGGGCATTGC 
TTTAACAACA ACAACAGCTG CAGTAGCGTC TCCGCCCAGA AGCCAAAGGC CCATCCTCTA 
GCTGCGCTGC AGAAACTGTG CGAGACCACG GATCCCCCAA GCACTGGCCT GCGCAGCGCG 
TCCTCCGCCG GATCCTCCAC AGCATCAGCA ACACTTCCCT CAGCGAACGG TAACGACCTG 
GTGGCCTTCA GCTGGGCCTG CAACGAGGCA GTTCTGAGTG CCAGTAATGG TGGCTCCGCC 
GGCGATTCGT CCATCATTAA GTGCTCCTAC TGTGATACCC CTTTTGCATC GAAGGGCGCC 
TACCGCCACC ATCTCTCCAA GGTGCACTTT GTGAAAGACG CAGGCGAGGA CTCGCCCAGG 
CTCAAGTCCC CCGCCGTTCA GAGCCCCAGG TCGATGCCAT TGGCCTCCCC AAGGAGATCA 
GCTTCCAGGA GTCCGGCGAC AGGATCTCAG CAGCCCCCTC CCTCGCCAAC CATTAGCCCC 
TATGACGAGA GTCCGCAGTC AAAGTTCCTC AAATACACGG AGCTGGCCAA ACAGCTATCC 
TCTAAAAACG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tio ( NM_001273750.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260679.1 Drosophila melanogaster tiptop (tio), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15199
Clone ID Related Accession (Same CDS sequence)	NM_079994.4 , NM_001273750.1
Accession Version	NM_001273750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tiptop, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCTGC ACGAGGCCGT CATGCTGGAA ATCTATCGCC AGGCGCTGAG TGCAAGTGAG 
TTAACTAGTC CGCGATGTCA GTCTCGCGAT TCCAACACCT CAGCTGGAGC GGGTGCTGGA 
ATGGCCGATG TCCGATGTCC ATCGAATGAG TCGCATTGTT CGGCGAACGA CCGTCTGACC 
CCAGCTGCAA CGCCCACTCT CACGCCTACT GAAGCAACCA TCTCCCCAAA CTCGGTGGGA 
CTTCCCCTGA CGGCGACCCT TCCTCCTGCA GCTGCAGTTG CTCTGCTACC GCCCCAATCC 
GCCGCCATGG CCGCTTACCT AGCCGCTGCC CAACAAAATC ACCTTCTGCT AACAAATCCA 
TTGGCGGCAG CAGCTTCACT GGTCCAACAT GCAACGCAGC AGGCAGTGGT GGAGGGGGAG 
GTGGAGTCGC CGGCCCTCGA TTTTAGCAGA AAGCGGCCTA AGAGCCACGG AGACGACGAT 
CAGGAAGAAG ATCAGGAACA GGACCAGGAA CAGGAGCAGG AGCAGGAGCC CGATCATGAT 
GTCCAGTGCG ATAATGGTCC TTTAGATCTA TCTGTAAGCA CAGGGAAAAG GCAAGAATCG 
GTTTCTCCGC CTGCCAGGAA GATTCCAAGA AGTATATCCG CAGATTACAA GTCACCTCTT 
CCACCTGGCA GCTGGATGCC CCCCATCAAC CCATACTTGG CAGCTGTAGC GGCAAAGACG 
GGCGGACTGG GATATTCTAA GTTAGCACCC AGTGAAGCCA GCAAAGCGTT GGAGAAGATG 
ACTGAGATGA GCCGATTGGA AACATCCCCA ACAGCTGCCC GAAGCTTAGG CGCCACATCA 
TCTGTAGGAG CAGGAGTGCC TGCGGGAGCT AGTTCCAACT CCGGTGGCCG GCACAGCGCG 
TGGCAGTCTC ATTGGCTGAA CAAAGGTGCT GATACTGCAA AGGATGTGTT TAAGTGTGTG 
TGGTGCAAGC AGAGCTTTTC AACTCTGGCT AACTTAACTG CCCACATGAA GGAGACTCAG 
CATTGCGGTG TGCAGATACC CTCGCCGTTG CCAACCGGAG GAGTCGGCAC TCCGTCTGCT 
CCTCCTCCCA CAAGGTTGGC CACTTCAGCC TCCAACTCGG CCTGTTCCAG CAGCAGTAGC 
TCCACCTCCA GCTCTTCAAA CTCCTCGAAA TCAGAGTTAA ATATGCTAAT TAAGGAAACT 
ATGCCCCTTC CCAGGAAATT GGTGAGGGGT CAGGATGTTT GGCTCGGAAA GGGTGCGGAG 
CAGACCCGCC AGATACTTAA GTGCATGTGG TGTGGACAAA GCTTCCGGTC TCTGGCCGAG 
ATGACCAGCC ACATGCAGGA GACGCAGCAC TACACGAATA TCATCTCGCA GGAGCAGATC 
ATTTCCTGGA AGTCGGGCGA TGAACGGGAG AGGCCGACCA ACACTGGAGT TCCGTCTACG 
TCCACCGCTG CTCCCTCTTC GCCGAGTTGC ACAGCTCCAT CAGTGAGTGC CGTGCTCACG 
TGTAAAGTTT GTGATCAGGC CTTCGGTAGC CTAAAGGAGT TAAGCACCCA TATGGCCCAA 
AAATCGCACT ACAAGGAGTC ACCGGCTCCG TCAGCATCTC CTCCAGCTGC CGGCACTGGC 
AATCCGAAAA GAGGAAGGCA AAATCGCAAC GAGAAACGCA AGAAATCCCT GCCGGTTAGG 
AAGCTTCTTG AGCTCGAGCG TTCCGGCTCG AATTCAAGTC TGGACTCAGC GCTAAAGCCC 
TTGAGGGACT TTGCTGCTGC CACCAAAATT ACCTGTGAAA AATGTGGCAG TAAGATTGAA 
ACTGCGTTGT TTGTCGAGCA CATACGAAAG TGCCTGGGCG AGAGTATTCC AATCCCTCCC 
CGTCGATCTA ACGCGGGAGT AGATCGCCTG CCAAGTCCCA GTCTAGGACT TGGCGCTGAA 
AAGCCACCAT CAGTCCTTAA TGCCCTGGAG CAACTTATAG AGAAGAGCTT CGAATCGAGA 
ACCAGCAGAA CCATGACACA TGGAGGCTAT TCCGAAGCTG GAACTCCGCT TGGAGCCAGC 
ATCCTAAAGC GTTTGGGAAT TGAGGACAGC AGTGACTACA CCAAGCCCCT TATGGATGCC 
CAGGCGATGC ACCTGCTCCG ATCCTCCTTC GCCTCGAGAG ACCGCAGTGC CAGCGAATCG 
AGCTCAGCCA GTAGGGTGGA ATCATCCTAC ACCCCAGACA GACAGCAGGC CACCCCCCAC 
AAAAGTCCAG ATACTCCTGC TCCGCCTCCT CCCCCACCGC CAACCATCAA AGCTGAACCA 
CTAGAGGCGG AACCCTTGGT GGGTTGCGAT CGGGAGGGCT GCAGTCCTCG GCAGCAGATC 
CAAGTGAAAA AGGAGTTCAG CATGGAAGCA TGTCGGGAGA GTCCTCGGTC TGTAAGCAAA 
TCACCTGCGC CGCAGACCGA AAGATCTCCA CCAGATAATG GCAGTCTACT GGCGTTGAAC 
TCCATGTTCG ATCAGCTCAG TGGAGTGGAG AACAGCGGCA ATAACAACTC GGGGCATTGC 
TTTAACAACA ACAACAGCTG CAGTAGCGTC TCCGCCCAGA AGCCAAAGGC CCATCCTCTA 
GCTGCGCTGC AGAAACTGTG CGAGACCACG GATCCCCCAA GCACTGGCCT GCGCAGCGCG 
TCCTCCGCCG GATCCTCCAC AGCATCAGCA ACACTTCCCT CAGCGAACGG TAACGACCTG 
GTGGCCTTCA GCTGGGCCTG CAACGAGGCA GTTCTGAGTG CCAGTAATGG TGGCTCCGCC 
GGCGATTCGT CCATCATTAA GTGCTCCTAC TGTGATACCC CTTTTGCATC GAAGGGCGCC 
TACCGCCACC ATCTCTCCAA GGTGCACTTT GTGAAAGACG CAGGCGAGGA CTCGCCCAGG 
CTCAAGTCCC CCGCCGTTCA GAGCCCCAGG TCGATGCCAT TGGCCTCCCC AAGGAGATCA 
GCTTCCAGGA GTCCGGCGAC AGGATCTCAG CAGCCCCCTC CCTCGCCAAC CATTAGCCCC 
TATGACGAGA GTCCGCAGTC AAAGTTCCTC AAATACACGG AGCTGGCCAA ACAGCTATCC 
TCTAAAAACG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260679.1
CDS	828..3902
Translation	MMLHEAVMLEIYRQALSASELTSPRCQSRDSNTSAGAGAGMADVRCPSNESHCSANDRLTPAATPTLTPTEATISPNSVGLPLTATLPPAAAVALLPPQSAAMAAYLAAAQQNHLLLTNPLAAAASLVQHATQQAVVEGEVESPALDFSRKRPKSHGDDDQEEDQEQDQEQEQEQEPDHDVQCDNGPLDLSVSTGKRQESVSPPARKIPRSISADYKSPLPPGSWMPPINPYLAAVAAKTGGLGYSKLAPSEASKALEKMTEMSRLETSPTAARSLGATSSVGAGVPAGASSNSGGRHSAWQSHWLNKGADTAKDVFKCVWCKQSFSTLANLTAHMKETQHCGVQIPSPLPTGGVGTPSAPPPTRLATSASNSACSSSSSSTSSSSNSSKSELNMLIKETMPLPRKLVRGQDVWLGKGAEQTRQILKCMWCGQSFRSLAEMTSHMQETQHYTNIISQEQIISWKSGDERERPTNTGVPSTSTAAPSSPSCTAPSVSAVLTCKVCDQAFGSLKELSTHMAQKSHYKESPAPSASPPAAGTGNPKRGRQNRNEKRKKSLPVRKLLELERSGSNSSLDSALKPLRDFAAATKITCEKCGSKIETALFVEHIRKCLGESIPIPPRRSNAGVDRLPSPSLGLGAEKPPSVLNALEQLIEKSFESRTSRTMTHGGYSEAGTPLGASILKRLGIEDSSDYTKPLMDAQAMHLLRSSFASRDRSASESSSASRVESSYTPDRQQATPHKSPDTPAPPPPPPPTIKAEPLEAEPLVGCDREGCSPRQQIQVKKEFSMEACRESPRSVSKSPAPQTERSPPDNGSLLALNSMFDQLSGVENSGNNNSGHCFNNNNSCSSVSAQKPKAHPLAALQKLCETTDPPSTGLRSASSAGSSTASATLPSANGNDLVAFSWACNEAVLSASNGGSAGDSSIIKCSYCDTPFASKGAYRHHLSKVHFVKDAGEDSPRLKSPAVQSPRSMPLASPRRSASRSPATGSQQPPPSPTISPYDESPQSKFLKYTELAKQLSSKNA

Target ORF information:

RefSeq Version	NM_001273750.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tiptop (tio), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273750.1

ORF Insert Sequence:

ATGATGCTGC ACGAGGCCGT CATGCTGGAA ATCTATCGCC AGGCGCTGAG TGCAAGTGAG 
TTAACTAGTC CGCGATGTCA GTCTCGCGAT TCCAACACCT CAGCTGGAGC GGGTGCTGGA 
ATGGCCGATG TCCGATGTCC ATCGAATGAG TCGCATTGTT CGGCGAACGA CCGTCTGACC 
CCAGCTGCAA CGCCCACTCT CACGCCTACT GAAGCAACCA TCTCCCCAAA CTCGGTGGGA 
CTTCCCCTGA CGGCGACCCT TCCTCCTGCA GCTGCAGTTG CTCTGCTACC GCCCCAATCC 
GCCGCCATGG CCGCTTACCT AGCCGCTGCC CAACAAAATC ACCTTCTGCT AACAAATCCA 
TTGGCGGCAG CAGCTTCACT GGTCCAACAT GCAACGCAGC AGGCAGTGGT GGAGGGGGAG 
GTGGAGTCGC CGGCCCTCGA TTTTAGCAGA AAGCGGCCTA AGAGCCACGG AGACGACGAT 
CAGGAAGAAG ATCAGGAACA GGACCAGGAA CAGGAGCAGG AGCAGGAGCC CGATCATGAT 
GTCCAGTGCG ATAATGGTCC TTTAGATCTA TCTGTAAGCA CAGGGAAAAG GCAAGAATCG 
GTTTCTCCGC CTGCCAGGAA GATTCCAAGA AGTATATCCG CAGATTACAA GTCACCTCTT 
CCACCTGGCA GCTGGATGCC CCCCATCAAC CCATACTTGG CAGCTGTAGC GGCAAAGACG 
GGCGGACTGG GATATTCTAA GTTAGCACCC AGTGAAGCCA GCAAAGCGTT GGAGAAGATG 
ACTGAGATGA GCCGATTGGA AACATCCCCA ACAGCTGCCC GAAGCTTAGG CGCCACATCA 
TCTGTAGGAG CAGGAGTGCC TGCGGGAGCT AGTTCCAACT CCGGTGGCCG GCACAGCGCG 
TGGCAGTCTC ATTGGCTGAA CAAAGGTGCT GATACTGCAA AGGATGTGTT TAAGTGTGTG 
TGGTGCAAGC AGAGCTTTTC AACTCTGGCT AACTTAACTG CCCACATGAA GGAGACTCAG 
CATTGCGGTG TGCAGATACC CTCGCCGTTG CCAACCGGAG GAGTCGGCAC TCCGTCTGCT 
CCTCCTCCCA CAAGGTTGGC CACTTCAGCC TCCAACTCGG CCTGTTCCAG CAGCAGTAGC 
TCCACCTCCA GCTCTTCAAA CTCCTCGAAA TCAGAGTTAA ATATGCTAAT TAAGGAAACT 
ATGCCCCTTC CCAGGAAATT GGTGAGGGGT CAGGATGTTT GGCTCGGAAA GGGTGCGGAG 
CAGACCCGCC AGATACTTAA GTGCATGTGG TGTGGACAAA GCTTCCGGTC TCTGGCCGAG 
ATGACCAGCC ACATGCAGGA GACGCAGCAC TACACGAATA TCATCTCGCA GGAGCAGATC 
ATTTCCTGGA AGTCGGGCGA TGAACGGGAG AGGCCGACCA ACACTGGAGT TCCGTCTACG 
TCCACCGCTG CTCCCTCTTC GCCGAGTTGC ACAGCTCCAT CAGTGAGTGC CGTGCTCACG 
TGTAAAGTTT GTGATCAGGC CTTCGGTAGC CTAAAGGAGT TAAGCACCCA TATGGCCCAA 
AAATCGCACT ACAAGGAGTC ACCGGCTCCG TCAGCATCTC CTCCAGCTGC CGGCACTGGC 
AATCCGAAAA GAGGAAGGCA AAATCGCAAC GAGAAACGCA AGAAATCCCT GCCGGTTAGG 
AAGCTTCTTG AGCTCGAGCG TTCCGGCTCG AATTCAAGTC TGGACTCAGC GCTAAAGCCC 
TTGAGGGACT TTGCTGCTGC CACCAAAATT ACCTGTGAAA AATGTGGCAG TAAGATTGAA 
ACTGCGTTGT TTGTCGAGCA CATACGAAAG TGCCTGGGCG AGAGTATTCC AATCCCTCCC 
CGTCGATCTA ACGCGGGAGT AGATCGCCTG CCAAGTCCCA GTCTAGGACT TGGCGCTGAA 
AAGCCACCAT CAGTCCTTAA TGCCCTGGAG CAACTTATAG AGAAGAGCTT CGAATCGAGA 
ACCAGCAGAA CCATGACACA TGGAGGCTAT TCCGAAGCTG GAACTCCGCT TGGAGCCAGC 
ATCCTAAAGC GTTTGGGAAT TGAGGACAGC AGTGACTACA CCAAGCCCCT TATGGATGCC 
CAGGCGATGC ACCTGCTCCG ATCCTCCTTC GCCTCGAGAG ACCGCAGTGC CAGCGAATCG 
AGCTCAGCCA GTAGGGTGGA ATCATCCTAC ACCCCAGACA GACAGCAGGC CACCCCCCAC 
AAAAGTCCAG ATACTCCTGC TCCGCCTCCT CCCCCACCGC CAACCATCAA AGCTGAACCA 
CTAGAGGCGG AACCCTTGGT GGGTTGCGAT CGGGAGGGCT GCAGTCCTCG GCAGCAGATC 
CAAGTGAAAA AGGAGTTCAG CATGGAAGCA TGTCGGGAGA GTCCTCGGTC TGTAAGCAAA 
TCACCTGCGC CGCAGACCGA AAGATCTCCA CCAGATAATG GCAGTCTACT GGCGTTGAAC 
TCCATGTTCG ATCAGCTCAG TGGAGTGGAG AACAGCGGCA ATAACAACTC GGGGCATTGC 
TTTAACAACA ACAACAGCTG CAGTAGCGTC TCCGCCCAGA AGCCAAAGGC CCATCCTCTA 
GCTGCGCTGC AGAAACTGTG CGAGACCACG GATCCCCCAA GCACTGGCCT GCGCAGCGCG 
TCCTCCGCCG GATCCTCCAC AGCATCAGCA ACACTTCCCT CAGCGAACGG TAACGACCTG 
GTGGCCTTCA GCTGGGCCTG CAACGAGGCA GTTCTGAGTG CCAGTAATGG TGGCTCCGCC 
GGCGATTCGT CCATCATTAA GTGCTCCTAC TGTGATACCC CTTTTGCATC GAAGGGCGCC 
TACCGCCACC ATCTCTCCAA GGTGCACTTT GTGAAAGACG CAGGCGAGGA CTCGCCCAGG 
CTCAAGTCCC CCGCCGTTCA GAGCCCCAGG TCGATGCCAT TGGCCTCCCC AAGGAGATCA 
GCTTCCAGGA GTCCGGCGAC AGGATCTCAG CAGCCCCCTC CCTCGCCAAC CATTAGCCCC 
TATGACGAGA GTCCGCAGTC AAAGTTCCTC AAATACACGG AGCTGGCCAA ACAGCTATCC 
TCTAAAAACG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tio cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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