Sep5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sep5
Entrez Gene ID	43990
Full Name	Septin 5
Synonyms	CG2916,Dmel\CG2916,Sep,sep5
General protein information	Preferred Names Septin 5 Names CG2916 gene product from transcript CG2916-RB CG2916-PA CG2916-PB Sep5-PA Sep5-PB Septin-5 septin septin 5
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 43F8-43F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_143704.3	NP_651961.1	septin 5, isoform B
NM_165578.3	NP_724632.1	septin 5, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SEPT11	XP_005639152.1
Bos taurus (cattle)	SEPT11	NP_001075916.1
Drosophila melanogaster (fruit fly)	Sep5	NP_651961.1
Homo sapiens (human)	SEPT11	NP_060713.1
Rattus norvegicus (Norway rat)	Sept11	NP_001100678.1
Gallus gallus (chicken)	SEPT11	XP_004941218.1
Danio rerio (zebrafish)	sept8b	NP_001077035.1
Pan troglodytes (chimpanzee)	SEPT11	XP_517208.3
Mus musculus (house mouse)	Sept11	NP_001009818.1
Xenopus tropicalis (tropical clawed frog)	sept11	NP_001136370.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It has been found that Sep2 is uniquely required for follicle cell encapsulation of female germline cysts, and that Sep2 and Sep5 are redundant for follicle cell proliferation.
Title: Partial Functional Diversification of Drosophila melanogaster Septin Genes Sep2 and Sep5.

Results show that Sep2 Sep5 double mutants have an early pupal lethal phenotype and lack imaginal discs, suggesting that these genes have redundant functions during imaginal cell proliferation.
Title: The Drosophila melanogaster septin gene Sep2 has a redundant function with the retrogene Sep5 in imaginal cell proliferation but is essential for oogenesis.

The following Sep5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sep5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11307	NM_165578.3 Latest version!	Drosophila melanogaster septin 5, transcript variant A (Sep5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa11307	NM_143704.3 Latest version!	Drosophila melanogaster septin 5, transcript variant B (Sep5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sep5 ( NM_143704.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651961.1 Drosophila melanogaster septin 5, transcript variant B (Sep5), mRNA.

Price & Availability↑

CloneID	OFa11307
Clone ID Related Accession (Same CDS sequence)	NM_143704.3 , NM_165578.3
Accession Version	NM_143704.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	septin 5, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_143704.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTCATT TAGTGACTGA AAAGAAGACG CAGGAGGTGG CAACCAAAGT CCCCAGGCTC 
CTTCAACTCG GCGGCCACGT GGGGTTCGAC ACCCTACCGG ACCAGCTGGT CAACAAGAGC 
GTCCAGAATG GCTTTAGCTT CAATATCCTA TGCATCGGCG AGACCGCTCT CGGGAAATCC 
ACTCTCATGG ACACACTGTT CAATACCAGT TTTGGATCTA CACCGAGCCC GCACAACCTG 
CCCAATGTGA AGCTGAAGGC AAATACATAC GAGCTGCAGG AGAGCAACGT CCGCCTGAAG 
CTGACCGTCT GTGACACGAT CGGATACGGA GATCAGGTTA ATAAAGCGGA CAGCTACAAG 
GCTTTGGTGG AGTACGTGGA CAGCCAGTTT GAAGCGTATT TGCAGGAAGA GCTCAAGATC 
CAGCGAGCGA TGGCCAGTGC TCATGATGGA CGCGTTCACG CCTGCCTGTA CTTCATCTGT 
CCCACGGGCC ATGGTCTGAA AGCTATGGAT TTGGTGTGCA TGAAGCAGCT TGACACTAGG 
GTGAACATCA TTCCCGTGAT CGCGAAGGCC GACACCATCT CCAAGTCGGA GCTGTCGGGG 
TTTAAGGAAC GCATAATGGA CGAACTACGG CGGAACAATG TGAGCATATA CCAGTTTCCC 
ATGGACGACG AAACCGTGTC CGAAACAAAT GCTGCGATGA ACGGCCATCT ACCCTTTGCC 
GTTGTTGGCA GTACGGAGTT TGTAAAGGTG GCCGGAAAGC AGGTTCGTGC CCGCCAATAT 
CCATGGGGAG CGGTGCACAT AGAAAACGAG GCGCACTGCG ACTTTGTGAA GCTGCGCGAG 
ATGCTCATTC GCACTAACAT GGAGGATCTG CGCGAACAGA CTCACACACG CCACTATGAG 
CTCTTCCGCC AGCGGCGCCT ACAACAAATG GGCTTTGTGG ACGTGGACAG CAACAACCAG 
CCGGTCTCCT TCCAACAGAC CTTCGAGTCG AAGCGATCCG ACCATCTGGC TTGCCTACAG 
GCCAAGGAGG AGGAAGTGCG CCAAATGTTC GTTCAGCGGG TCAAGCAGAA GGAGAACGAG 
CTGAAGGACA ACGAGAAGGA ATTGCATACT AAGTTCGACC GGCTAAAACG GGAGCATTTG 
GAGGAAAAGG CCCAACTGGA AGAGGCCCGT CGGCAGCTGG AAGAGGATTG CCAGGAGCTA 
CAGCGTCGTA GGCTCCAGAT GGCCAATGGT TCGCACACTC TCACCTTGGG CAGGGGCAAG 
AAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651961.1
CDS	213..1481
Translation	MVHLVTEKKTQEVATKVPRLLQLGGHVGFDTLPDQLVNKSVQNGFSFNILCIGETALGKSTLMDTLFNTSFGSTPSPHNLPNVKLKANTYELQESNVRLKLTVCDTIGYGDQVNKADSYKALVEYVDSQFEAYLQEELKIQRAMASAHDGRVHACLYFICPTGHGLKAMDLVCMKQLDTRVNIIPVIAKADTISKSELSGFKERIMDELRRNNVSIYQFPMDDETVSETNAAMNGHLPFAVVGSTEFVKVAGKQVRARQYPWGAVHIENEAHCDFVKLREMLIRTNMEDLREQTHTRHYELFRQRRLQQMGFVDVDSNNQPVSFQQTFESKRSDHLACLQAKEEEVRQMFVQRVKQKENELKDNEKELHTKFDRLKREHLEEKAQLEEARRQLEEDCQELQRRRLQMANGSHTLTLGRGKKK

Target ORF information:

RefSeq Version	NM_143704.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster septin 5, transcript variant B (Sep5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143704.3

ORF Insert Sequence:

ATGGTTCATT TAGTGACTGA AAAGAAGACG CAGGAGGTGG CAACCAAAGT CCCCAGGCTC 
CTTCAACTCG GCGGCCACGT GGGGTTCGAC ACCCTACCGG ACCAGCTGGT CAACAAGAGC 
GTCCAGAATG GCTTTAGCTT CAATATCCTA TGCATCGGCG AGACCGCTCT CGGGAAATCC 
ACTCTCATGG ACACACTGTT CAATACCAGT TTTGGATCTA CACCGAGCCC GCACAACCTG 
CCCAATGTGA AGCTGAAGGC AAATACATAC GAGCTGCAGG AGAGCAACGT CCGCCTGAAG 
CTGACCGTCT GTGACACGAT CGGATACGGA GATCAGGTTA ATAAAGCGGA CAGCTACAAG 
GCTTTGGTGG AGTACGTGGA CAGCCAGTTT GAAGCGTATT TGCAGGAAGA GCTCAAGATC 
CAGCGAGCGA TGGCCAGTGC TCATGATGGA CGCGTTCACG CCTGCCTGTA CTTCATCTGT 
CCCACGGGCC ATGGTCTGAA AGCTATGGAT TTGGTGTGCA TGAAGCAGCT TGACACTAGG 
GTGAACATCA TTCCCGTGAT CGCGAAGGCC GACACCATCT CCAAGTCGGA GCTGTCGGGG 
TTTAAGGAAC GCATAATGGA CGAACTACGG CGGAACAATG TGAGCATATA CCAGTTTCCC 
ATGGACGACG AAACCGTGTC CGAAACAAAT GCTGCGATGA ACGGCCATCT ACCCTTTGCC 
GTTGTTGGCA GTACGGAGTT TGTAAAGGTG GCCGGAAAGC AGGTTCGTGC CCGCCAATAT 
CCATGGGGAG CGGTGCACAT AGAAAACGAG GCGCACTGCG ACTTTGTGAA GCTGCGCGAG 
ATGCTCATTC GCACTAACAT GGAGGATCTG CGCGAACAGA CTCACACACG CCACTATGAG 
CTCTTCCGCC AGCGGCGCCT ACAACAAATG GGCTTTGTGG ACGTGGACAG CAACAACCAG 
CCGGTCTCCT TCCAACAGAC CTTCGAGTCG AAGCGATCCG ACCATCTGGC TTGCCTACAG 
GCCAAGGAGG AGGAAGTGCG CCAAATGTTC GTTCAGCGGG TCAAGCAGAA GGAGAACGAG 
CTGAAGGACA ACGAGAAGGA ATTGCATACT AAGTTCGACC GGCTAAAACG GGAGCATTTG 
GAGGAAAAGG CCCAACTGGA AGAGGCCCGT CGGCAGCTGG AAGAGGATTG CCAGGAGCTA 
CAGCGTCGTA GGCTCCAGAT GGCCAATGGT TCGCACACTC TCACCTTGGG CAGGGGCAAG 
AAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sep5 ( NM_165578.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724632.1 Drosophila melanogaster septin 5, transcript variant A (Sep5), mRNA.

Price & Availability↑

CloneID	OFa11307
Clone ID Related Accession (Same CDS sequence)	NM_143704.3 , NM_165578.3
Accession Version	NM_165578.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	septin 5, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165578.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTCATT TAGTGACTGA AAAGAAGACG CAGGAGGTGG CAACCAAAGT CCCCAGGCTC 
CTTCAACTCG GCGGCCACGT GGGGTTCGAC ACCCTACCGG ACCAGCTGGT CAACAAGAGC 
GTCCAGAATG GCTTTAGCTT CAATATCCTA TGCATCGGCG AGACCGCTCT CGGGAAATCC 
ACTCTCATGG ACACACTGTT CAATACCAGT TTTGGATCTA CACCGAGCCC GCACAACCTG 
CCCAATGTGA AGCTGAAGGC AAATACATAC GAGCTGCAGG AGAGCAACGT CCGCCTGAAG 
CTGACCGTCT GTGACACGAT CGGATACGGA GATCAGGTTA ATAAAGCGGA CAGCTACAAG 
GCTTTGGTGG AGTACGTGGA CAGCCAGTTT GAAGCGTATT TGCAGGAAGA GCTCAAGATC 
CAGCGAGCGA TGGCCAGTGC TCATGATGGA CGCGTTCACG CCTGCCTGTA CTTCATCTGT 
CCCACGGGCC ATGGTCTGAA AGCTATGGAT TTGGTGTGCA TGAAGCAGCT TGACACTAGG 
GTGAACATCA TTCCCGTGAT CGCGAAGGCC GACACCATCT CCAAGTCGGA GCTGTCGGGG 
TTTAAGGAAC GCATAATGGA CGAACTACGG CGGAACAATG TGAGCATATA CCAGTTTCCC 
ATGGACGACG AAACCGTGTC CGAAACAAAT GCTGCGATGA ACGGCCATCT ACCCTTTGCC 
GTTGTTGGCA GTACGGAGTT TGTAAAGGTG GCCGGAAAGC AGGTTCGTGC CCGCCAATAT 
CCATGGGGAG CGGTGCACAT AGAAAACGAG GCGCACTGCG ACTTTGTGAA GCTGCGCGAG 
ATGCTCATTC GCACTAACAT GGAGGATCTG CGCGAACAGA CTCACACACG CCACTATGAG 
CTCTTCCGCC AGCGGCGCCT ACAACAAATG GGCTTTGTGG ACGTGGACAG CAACAACCAG 
CCGGTCTCCT TCCAACAGAC CTTCGAGTCG AAGCGATCCG ACCATCTGGC TTGCCTACAG 
GCCAAGGAGG AGGAAGTGCG CCAAATGTTC GTTCAGCGGG TCAAGCAGAA GGAGAACGAG 
CTGAAGGACA ACGAGAAGGA ATTGCATACT AAGTTCGACC GGCTAAAACG GGAGCATTTG 
GAGGAAAAGG CCCAACTGGA AGAGGCCCGT CGGCAGCTGG AAGAGGATTG CCAGGAGCTA 
CAGCGTCGTA GGCTCCAGAT GGCCAATGGT TCGCACACTC TCACCTTGGG CAGGGGCAAG 
AAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724632.1
CDS	273..1541
Translation	MVHLVTEKKTQEVATKVPRLLQLGGHVGFDTLPDQLVNKSVQNGFSFNILCIGETALGKSTLMDTLFNTSFGSTPSPHNLPNVKLKANTYELQESNVRLKLTVCDTIGYGDQVNKADSYKALVEYVDSQFEAYLQEELKIQRAMASAHDGRVHACLYFICPTGHGLKAMDLVCMKQLDTRVNIIPVIAKADTISKSELSGFKERIMDELRRNNVSIYQFPMDDETVSETNAAMNGHLPFAVVGSTEFVKVAGKQVRARQYPWGAVHIENEAHCDFVKLREMLIRTNMEDLREQTHTRHYELFRQRRLQQMGFVDVDSNNQPVSFQQTFESKRSDHLACLQAKEEEVRQMFVQRVKQKENELKDNEKELHTKFDRLKREHLEEKAQLEEARRQLEEDCQELQRRRLQMANGSHTLTLGRGKKK

Target ORF information:

RefSeq Version	NM_165578.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster septin 5, transcript variant A (Sep5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165578.3

ORF Insert Sequence:

ATGGTTCATT TAGTGACTGA AAAGAAGACG CAGGAGGTGG CAACCAAAGT CCCCAGGCTC 
CTTCAACTCG GCGGCCACGT GGGGTTCGAC ACCCTACCGG ACCAGCTGGT CAACAAGAGC 
GTCCAGAATG GCTTTAGCTT CAATATCCTA TGCATCGGCG AGACCGCTCT CGGGAAATCC 
ACTCTCATGG ACACACTGTT CAATACCAGT TTTGGATCTA CACCGAGCCC GCACAACCTG 
CCCAATGTGA AGCTGAAGGC AAATACATAC GAGCTGCAGG AGAGCAACGT CCGCCTGAAG 
CTGACCGTCT GTGACACGAT CGGATACGGA GATCAGGTTA ATAAAGCGGA CAGCTACAAG 
GCTTTGGTGG AGTACGTGGA CAGCCAGTTT GAAGCGTATT TGCAGGAAGA GCTCAAGATC 
CAGCGAGCGA TGGCCAGTGC TCATGATGGA CGCGTTCACG CCTGCCTGTA CTTCATCTGT 
CCCACGGGCC ATGGTCTGAA AGCTATGGAT TTGGTGTGCA TGAAGCAGCT TGACACTAGG 
GTGAACATCA TTCCCGTGAT CGCGAAGGCC GACACCATCT CCAAGTCGGA GCTGTCGGGG 
TTTAAGGAAC GCATAATGGA CGAACTACGG CGGAACAATG TGAGCATATA CCAGTTTCCC 
ATGGACGACG AAACCGTGTC CGAAACAAAT GCTGCGATGA ACGGCCATCT ACCCTTTGCC 
GTTGTTGGCA GTACGGAGTT TGTAAAGGTG GCCGGAAAGC AGGTTCGTGC CCGCCAATAT 
CCATGGGGAG CGGTGCACAT AGAAAACGAG GCGCACTGCG ACTTTGTGAA GCTGCGCGAG 
ATGCTCATTC GCACTAACAT GGAGGATCTG CGCGAACAGA CTCACACACG CCACTATGAG 
CTCTTCCGCC AGCGGCGCCT ACAACAAATG GGCTTTGTGG ACGTGGACAG CAACAACCAG 
CCGGTCTCCT TCCAACAGAC CTTCGAGTCG AAGCGATCCG ACCATCTGGC TTGCCTACAG 
GCCAAGGAGG AGGAAGTGCG CCAAATGTTC GTTCAGCGGG TCAAGCAGAA GGAGAACGAG 
CTGAAGGACA ACGAGAAGGA ATTGCATACT AAGTTCGACC GGCTAAAACG GGAGCATTTG 
GAGGAAAAGG CCCAACTGGA AGAGGCCCGT CGGCAGCTGG AAGAGGATTG CCAGGAGCTA 
CAGCGTCGTA GGCTCCAGAT GGCCAATGGT TCGCACACTC TCACCTTGGG CAGGGGCAAG 
AAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sep5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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