spag cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	spag
Entrez Gene ID	43958
Full Name	spaghetti
Synonyms	CG13570,Dmel\CG13570,Spag,l(2)k12101
General protein information	Preferred Names spaghetti Names CG13570 gene product from transcript CG13570-RA CG13570-PA spag-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60C1-60C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_079925.3	NP_524664.1	spaghetti

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RPAP3	XP_005636987.1
	RPAP3	XP_001097194.1
Mus musculus (house mouse)	Rpap3	NP_082279.1
Gallus gallus (chicken)	RPAP3	NP_001006231.1
Danio rerio (zebrafish)	rpap3	NP_956498.2
Xenopus tropicalis (tropical clawed frog)	rpap3	NP_001016680.1
Pan troglodytes (chimpanzee)	RPAP3	XP_509021.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005014	XP_315121.4
Arabidopsis thaliana (thale cress)	AT1G56440	NP_176039.2
Oryza sativa (rice)	Os05g0129900	NP_001054548.1
Drosophila melanogaster (fruit fly)	spag	NP_524664.1
Homo sapiens (human)	RPAP3	NP_078880.2
Bos taurus (cattle)	RPAP3	NP_001095400.2
Rattus norvegicus (Norway rat)	Rpap3	NP_001004243.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that the HSP-90 cochaperone spaghetti protein (SPAG) antagonizes casein kinase I epsilon DBT autophosphorylation in S2 cells
Title: Drosophila DBT Autophosphorylation of Its C-Terminal Domain Antagonized by SPAG and Involved in UV-Induced Apoptosis.

we show that spag knock-down or reduced Dbt activity in circadian cells leads to longer circadian periods and expression of activated initiator caspase only during the middle of the day.
Title: Drosophila spaghetti and doubletime link the circadian clock and light to caspases, apoptosis and tauopathy.

Interaction of Spag with both Hsp70 and Hsp90 suggests a model whereby R2TP would accompany clients from Hsp70 to Hsp90 to facilitate their assembly into macromolecular complexes.
Title: Drosophila Spag is the homolog of RNA polymerase II-associated protein 3 (RPAP3) and recruits the heat shock proteins 70 and 90 (Hsp70 and Hsp90) during the assembly of cellular machineries.

The following spag gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spag cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11306	NM_079925.3 Latest version!	Drosophila melanogaster spaghetti (spag), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spag ( NM_079925.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524664.1 Drosophila melanogaster spaghetti (spag), mRNA.

Price & Availability↑

CloneID	OFa11306
Clone ID Related Accession (Same CDS sequence)	NM_079925.3
Accession Version	NM_079925.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spaghetti
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079925.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGCAC AGGAAAAAGC CTTCGAACTG CAGCGCCAGG TGCGCCAGAA CGCGCGGGAG 
TACGAGAACT CCGTGAAGGA CTTGTACTCC TGGGAGCAAG ACATTAAAAA CAAGGAGAAA 
GAGCTCCAGA AGTCGCCGCT TTCTGCCGCC AATAAGGATC TGCCAGTGCG CAGTCATGTC 
CAGACAGACA AGTCGCGGAA GGAGAGCCCA TCCTCCTCGG CGGCCAGCTC ACCCACGGAG 
AAACAGGACT TGCCAGTGGA TCCTGTGGCC CAGCAGTACA AGAAGGCCAA CGACATCAAG 
GACCGTGGAA ACACCTATGT CAAGCAGGGG GAGTACGAGA AGGCCATCGT GGCCTACTCC 
ACTGCCATTG CCGTCTATCC CCACGATCCC ATTTACCACA TTAACCGGGC ACTGTGCTAC 
CTAAAACAGG AGAGCTTTGA TCAATGCGTG GAGGACTGCG AGGCCGCCAT TGCGCTGGAT 
AAGCTCTGCG TGAAAGCTTA CTACCGGCGA ATGCAGGCCA ACGAGTCCCT GGGCAACAAC 
ATGGAAGCCC TGAAGGATTG CACCACTGTG CTGGCCATCG AGCCGAAGAA CATCGAGGCC 
AAGAGGAGCC TGGCCAGAAT CAACGACCGT CTGCGCAAGA TTGCCACCAA AAGTGGGCCA 
AACTTTACAC CCGATCGTCC TGGCATGATT GAGATCTTGC CAATCGAGAA GCCTGCTTAT 
AAACGCTCCA AGAAGGCAAT GCGCAGTGTT CCCGTTGTAG ACGTGGTATC TCCTCGTGCT 
ACCATCGATG ATAGCAACCA ACTACGCATT TCGGATGAGG ACATAGATAA GATATTTAAT 
AGTAACTGCG GCATAATCGA AGAGGTCAAA AAAACAAATC CAAAGCCGAC GCCAATGCCA 
GACACATCAG GTCCACCCAA AGCTGAGACC ATTGCTAAGA CCTCCAAAGA AGTCAAGCCT 
ACCAAACAGA CTGCGGTTAA AGTAGCTCCA GCTGTGGAAA CCCCCAAAGA AACAGAAACA 
CGCAAGGATA CCAAGATTGT GCCTGAATCC GATAATGAGG CCAAGCCAAG TGCACCCAAA 
AAAACGGCTG TCGAGGTCCC CAAGGTTCAG ACTCAAGTTT CTCCACCAAA GACCACAATA 
GAACGCAGCC CAGAAGTCAA TACCGTGCAA ACTGAAAAAA TTGAACAAGC TTCGAGCAAC 
AACGCAATGT CACCCAGTCC AATAGAGCGC TTCTTACCGC CCGCACCCAC GAGCACAGCA 
CAGTTCCATG TGACCTGGAA GGAGCTGAGT GGTCCGCAGA AGTACCAATA CCTAAAGTCC 
ATTGAAGTCC CGAATCTGTG TAAGATCCTG GGAGCAGGCT TCGATTCGGA CACGTTTGCC 
GACCTGCTGC GCACCATCCA CGACTTCTTT GTACCAAATA AGGAGCCAAA CACAGCAGCT 
GTCTTGCTGG AGATCAGCAA AAATGACGAA TTCACCATTT TGGCCATGCT CATGTCCGCA 
GAGGAGAAGA AAATGGTTTC GTCTATCCTC AACGCCATCA AGAACTGGCC CAGCAAGAAT 
CCGGCTGTGC TGGACAACCT TTTCAAGGAA TACGGAGTAG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524664.1
CDS	88..1692
Translation	MSAQEKAFELQRQVRQNAREYENSVKDLYSWEQDIKNKEKELQKSPLSAANKDLPVRSHVQTDKSRKESPSSSAASSPTEKQDLPVDPVAQQYKKANDIKDRGNTYVKQGEYEKAIVAYSTAIAVYPHDPIYHINRALCYLKQESFDQCVEDCEAAIALDKLCVKAYYRRMQANESLGNNMEALKDCTTVLAIEPKNIEAKRSLARINDRLRKIATKSGPNFTPDRPGMIEILPIEKPAYKRSKKAMRSVPVVDVVSPRATIDDSNQLRISDEDIDKIFNSNCGIIEEVKKTNPKPTPMPDTSGPPKAETIAKTSKEVKPTKQTAVKVAPAVETPKETETRKDTKIVPESDNEAKPSAPKKTAVEVPKVQTQVSPPKTTIERSPEVNTVQTEKIEQASSNNAMSPSPIERFLPPAPTSTAQFHVTWKELSGPQKYQYLKSIEVPNLCKILGAGFDSDTFADLLRTIHDFFVPNKEPNTAAVLLEISKNDEFTILAMLMSAEEKKMVSSILNAIKNWPSKNPAVLDNLFKEYGVA

Target ORF information:

RefSeq Version	NM_079925.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spaghetti (spag), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079925.3

ORF Insert Sequence:

ATGAGTGCAC AGGAAAAAGC CTTCGAACTG CAGCGCCAGG TGCGCCAGAA CGCGCGGGAG 
TACGAGAACT CCGTGAAGGA CTTGTACTCC TGGGAGCAAG ACATTAAAAA CAAGGAGAAA 
GAGCTCCAGA AGTCGCCGCT TTCTGCCGCC AATAAGGATC TGCCAGTGCG CAGTCATGTC 
CAGACAGACA AGTCGCGGAA GGAGAGCCCA TCCTCCTCGG CGGCCAGCTC ACCCACGGAG 
AAACAGGACT TGCCAGTGGA TCCTGTGGCC CAGCAGTACA AGAAGGCCAA CGACATCAAG 
GACCGTGGAA ACACCTATGT CAAGCAGGGG GAGTACGAGA AGGCCATCGT GGCCTACTCC 
ACTGCCATTG CCGTCTATCC CCACGATCCC ATTTACCACA TTAACCGGGC ACTGTGCTAC 
CTAAAACAGG AGAGCTTTGA TCAATGCGTG GAGGACTGCG AGGCCGCCAT TGCGCTGGAT 
AAGCTCTGCG TGAAAGCTTA CTACCGGCGA ATGCAGGCCA ACGAGTCCCT GGGCAACAAC 
ATGGAAGCCC TGAAGGATTG CACCACTGTG CTGGCCATCG AGCCGAAGAA CATCGAGGCC 
AAGAGGAGCC TGGCCAGAAT CAACGACCGT CTGCGCAAGA TTGCCACCAA AAGTGGGCCA 
AACTTTACAC CCGATCGTCC TGGCATGATT GAGATCTTGC CAATCGAGAA GCCTGCTTAT 
AAACGCTCCA AGAAGGCAAT GCGCAGTGTT CCCGTTGTAG ACGTGGTATC TCCTCGTGCT 
ACCATCGATG ATAGCAACCA ACTACGCATT TCGGATGAGG ACATAGATAA GATATTTAAT 
AGTAACTGCG GCATAATCGA AGAGGTCAAA AAAACAAATC CAAAGCCGAC GCCAATGCCA 
GACACATCAG GTCCACCCAA AGCTGAGACC ATTGCTAAGA CCTCCAAAGA AGTCAAGCCT 
ACCAAACAGA CTGCGGTTAA AGTAGCTCCA GCTGTGGAAA CCCCCAAAGA AACAGAAACA 
CGCAAGGATA CCAAGATTGT GCCTGAATCC GATAATGAGG CCAAGCCAAG TGCACCCAAA 
AAAACGGCTG TCGAGGTCCC CAAGGTTCAG ACTCAAGTTT CTCCACCAAA GACCACAATA 
GAACGCAGCC CAGAAGTCAA TACCGTGCAA ACTGAAAAAA TTGAACAAGC TTCGAGCAAC 
AACGCAATGT CACCCAGTCC AATAGAGCGC TTCTTACCGC CCGCACCCAC GAGCACAGCA 
CAGTTCCATG TGACCTGGAA GGAGCTGAGT GGTCCGCAGA AGTACCAATA CCTAAAGTCC 
ATTGAAGTCC CGAATCTGTG TAAGATCCTG GGAGCAGGCT TCGATTCGGA CACGTTTGCC 
GACCTGCTGC GCACCATCCA CGACTTCTTT GTACCAAATA AGGAGCCAAA CACAGCAGCT 
GTCTTGCTGG AGATCAGCAA AAATGACGAA TTCACCATTT TGGCCATGCT CATGTCCGCA 
GAGGAGAAGA AAATGGTTTC GTCTATCCTC AACGCCATCA AGAACTGGCC CAGCAAGAAT 
CCGGCTGTGC TGGACAACCT TTTCAAGGAA TACGGAGTAG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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spag cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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