lgs cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	lgs
Entrez Gene ID	43791
Full Name	legless
Synonyms	BCL9,CG2041,Dm Lgs,Dmel\CG2041,Lgl,Lgs
General protein information	Preferred Names legless Names CG2041 gene product from transcript CG2041-RA CG2041-PA lgs-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102B8-102C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_143665.4	NP_651922.1	legless

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the PHD domain is required to link Drosophila Pygopus to Legless/beta-catenin and not to histone H3
Title: The PHD domain is required to link Drosophila Pygopus to Legless/beta-catenin and not to histone H3.

Data show that human and Drosophila Pygo PHD fingers associate with their cognate HD1 domains from BCL9/Legless to bind specifically to the histone H3 tail methylated at lysine 4 (H3K4me).
Title: Decoding of methylated histone H3 tail by the Pygo-BCL9 Wnt signaling complex.

Pygopus and legless provide essential transcriptional coactivator functions to armadillo/beta-catenin.
Title: Pygopus and legless provide essential transcriptional coactivator functions to armadillo/beta-catenin.

Pygo and Lgs function in targeting Armadillo/beta-catenin to the nucleus, thus ensuring its availability to TCF during Wnt signalling.
Title: Pygopus and Legless target Armadillo/beta-catenin to the nucleus to enable its transcriptional co-activator function.

identifed conserved residues within Pygopus that are required for binding to Legless in votro
Title: Pygopus residues required for its binding to Legless are critical for transcription and development.

The following lgs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lgs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00265	NM_143665.4 Latest version!	Drosophila melanogaster legless (lgs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lgs ( NM_143665.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651922.1 Drosophila melanogaster legless (lgs), mRNA.

Price & Availability↑

CloneID	OFa00265
Clone ID Related Accession (Same CDS sequence)	NM_143665.4
Accession Version	NM_143665.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	legless
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_143665.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCTCGA CAACAATGCC CCGCAGTCCA ACCCAACAAC AGCCGCAACC AAACTCCGAT 
GCCTCCTCAA CAAGTGCATC TGGATCAAAT CCTGGAGCAG CGATCGGAAA TGGGGACTCG 
GCGGCGAGCA GAAGTTCTCC GAAGACCCTT AATAGCGAAC CCTTTTCTAC TTTGTCGCCG 
GATCAAATAA AATTGACGCC AGAAGAAGGC ACTGAGAAAA GCGGACTATC AACTAGTGAT 
AAAGCTGCCA CTGGAGGAGC CCCAGGCAGT GGAAATAATC TGCCCGAGGG ACAAACTATG 
CTAAGGCAGA ACTCTACGAG CACAATCAAC TCGTGCCTAG TCGCTTCTCC ACAAAACTCC 
AGTGAACACT CGAATAGCAG CAATGTGTCT GCTACAGTGG GCCTTACTCA GATGGTAGAT 
TGTGACGAGC AATCGAAGAA AAACAAATGT AGTGTGAAGG ACGAGGAAGC TGAAATAAGT 
TCTAATAAAG CAAAAGGTCA AGCAGCTGGT GGCGGCTGCG AAACAGGTTC TACATCCAGT 
TTGACTGTCA AGGAAGAACC CACCGATGTC TTAGGCAGTT TAGTAAATAT GAAAAAAGAA 
GAAAGAGAAA ATCATTCGCC AACGATGTCC CCTGTTGGTT TTGGTTCAAT TGGTAATGCA 
CAGGACAACT CCGCTACACC GGTTAAAATT GAAAGAATTT CAAACGACAG TACCACGGAA 
AAAAAAGGAT CGTCCTTGAC AATGAATAAT GACGAAATGA GCATGGAAGG CTGCAATCAG 
TTGAATCCCG ATTTTATCAA TGAATCTTTA AATAATCCTG CAATTTCGAG CATATTAGTA 
AGCGGAGTAG GACCAATACC CGGAATCGGA GTTGGAGCGG GGACGGGAAA TTTATTGACT 
GCCAACGCCA ATGGAATCTC CTCGGGTAGC AGTAATTGTT TGGATTACAT GCAACAGCAA 
AATCACATAT TCGTGTTTTC AACTCAGCTG GCCAACAAAG GGGCCGAATC AGTTTTAAGC 
GGTCAATTTC AAACTATTAT TGCGTATCAC TGCACTCAGC CTGCTACAAA AAGCTTCCTG 
GAAGACTTTT TTATGAAAAA CCCTTTAAAG ATTAACAAGT TACAGCGGCA CAATTCCGTC 
GGTATGCCAT GGATAGGCAT GGGGCAGGTT GGACTAACTC CTCCTAATCC TGTAGCCAAA 
ATAACACAAC AGCAGCCACA TACAAAGACC GTAGGCCTAT TGAAACCCCA ATTCAATCAA 
CATGAAAACA GCAAACGTAG TACTGTAAGC GCGCCTAGCA ACTCTTTTGT CGACCAGTCT 
GATCCTATGG GCAACGAAAC TGAATTGATG TGCTGGGAAG GCGGATCCTC AAACACCAGT 
AGGTCTGGAC AAAACTCACG AAATCATGTA GACAGTATCA GTACATCCAG CGAGTCACAG 
GCAATAAAGA TACTGGAAGC AGCTGGCGTT GATTTGGGAC AGGTCACAAA AGGAAGCGAT 
CCTGGCCTGA CAACTGAAAA CAACATTGTA TCACTGCAAG GAGTTAAGGT TCCAGACGAA 
AACCTTACAC CACAACAGCG GCAACATCGG GAAGAACAGT TGGCAAAAAT AAAAAAAATG 
AATCAATTTC TTTTTCCTGA AAATGAGAAT TCAGTAGGAG CTAATGTAAG CTCACAGATA 
ACAAAAATTC CAGGAGATTT AATGATGGGG ATGTCGGGTG GCGGAGGCGG ATCTATTATA 
AATCCGACGA TGCGACAACT GCATATGCCA GGTAACGCCA AATCGGAGCT CTTATCGGCG 
ACAAGTTCAG GACTTTCGGA AGATGTAATG CATCCAGGGG ATGTTATATC AGATATGGGT 
GCCGTAATAG GATGTAATAA TAATCAAAAA ACCAGTGTGC AATGTGGATC TGGAGTAGGT 
GTTGTCACTG GAACAACTGC AGCTGGAGTA AATGTCAATA TGCATTGCTC AAGCTCCGGC 
GCCCCGAATG GCAATATGAT GGGAAGCTCT ACGGATATGC TAGCCTCGTT TGGCAACACA 
AGCTGCAACG TCATCGGAAC GGCCCCAGAT ATGTCTAAGG AAGTTTTAAA TCAAGATAGC 
CGAACCCATT CACATCAAGG GGGAGTTGCT CAAATGGAGT GGTCGAAGAT TCAACATCAA 
TTTTTCGAAG AACGCCTCAA GGGGGGCAAG CCCAGACAAG TCACTGGAAC TGTAGTACCA 
CAACAGCAAA CCCCTTCTGG ATCTGGTGGA AACTCGTTAA ACAACCAGGT GCGACCCCTG 
CAAGGTCCAC CTCCTCCTTA CCACTCCATC CAGAGATCTG CGTCAGTACC AATAGCCACT 
CAATCGCCCA ATCCCTCGAG TCCAAACAAT CTATCTCTCC CGTCACCGCG GACAACCGCA 
GCAGTCATGG GATTGCCGAC CAACTCTCCT AGCATGGATG GAACAGGATC ATTATCTGGA 
TCTGTTCCGC AAGCTAATAC TTCGACGGTT CAGGCAGGCA CAACAACAGT GCTCTCAGCA 
AACAAGAACT GTTTTCAGGC AGACACCCCA TCGCCGTCAA ATCAAAATCG TAGTAGAAAT 
ACCGGATCGT CAAGCGTTCT TACGCATAAC TTAAGCAGCA ACCCAAGTAC CCCCTTATCT 
CATCTATCCC CAAAGGAATT TGAGTCTTTC GGTCAGTCCT CTGCTGGTGA TAACATGAAA 
AGTAGGCGAC CAAGCCCACA GGGTCAGCGG TCACCAGTAA ATAGTCTAAT AGAGGCAAAT 
AAAGATGTAC GATTTGCTGC ATCCAGTCCT GGTTTTAACC CGCATCCACA TATGCAAAGC 
AATTCAAATT CAGCATTAAA CGCCTATAAA ATGGGCTCTA CCAATATACA GATGGAGCGT 
CAAGCATCAG CGCAAGGTGG ATCCGTACAA TTTAGTCGGC GCTCCGATAA TATTCCGCTA 
AATCCCAATA GTGGCAATCG GCCGCCACCA AACAAGATGA CCCAAAACTT CGATCCAATC 
TCTTCTTTGG CACAAATGTC CCAACAACTA ACAAGTTGCG TGTCCAGCAT GGGTAGTCCA 
GCCGGAACTG GTGGTATGAC GATGATGGGG GGTCCGGGAC CGTCCGACAT CAATATTGAG 
CATGGAATAA TTTCGGGACT AGATGGATCA GGAATAGATA CCATAAATCA AAATAACTGT 
CATTCAATGA ATGTCGTAAT GAACTCAATG GGTCCCCGAA TGCTGAATCC TAAAATGTGC 
GTAGCAGGCG GTCCAAATGG ACCGCCTGGC TTTAATCCTA ATTCCCCCAA TGGTGGATTA 
AGAGAGAATT CCATAGGGTC TGGCTGTGGC TCAGCAAACT CTTCAAACTT TCAAGGGGTT 
GTTCCACCTG GTGCCAGAAT GATGGGTCGA ATGCCAGTCA ATTTTGGTTC GAATTTCAAT 
CCGAATATTC AGGTAAAGGC GAGTACCCCA AACACCATAC AATACATGCC AGTAAGGGCA 
CAGAACGCCA ACAACAATAA CAACAATGGA GCTAATAATG TGCGAATGCC ACCTAGTCTG 
GAATTTTTGC AGAGGTACGC TAACCCTCAA ATGGGTGCTG TAGGCAATGG GTCGCCAATA 
TGCCCACCAT CAGCCAGCGA CGGTACTCCT GGAATGCCAG GATTGATGGC GGGACCAGGA 
GCCGGAGGTA TGCTAATGAA TTCTTCCGGA GAGCAACACC AGAACAAGAT CACAAACAAT 
CCTGGGGCAA GCAATGGTAT TAACTTCTTT CAGAATTGCA ATCAAATGTC TATTGTTGAC 
GAAGAGGGTG GATTACCCGG CCATGACGGA TCAATGAATA TTGGTCAACC ATCTATGATA 
AGGGGCATGC GTCCACATGC CATGCGGCCA AATGTAATGG GTGCGCGGAT GCCACCCGTT 
AACAGGCAAA TTCAGTTTGC ACAGTCATCG GATGGTATTG ACTGTGTCGG GGATCCGTCA 
TCATTTTTCA CTAACGCTTC CTGCAACAGC GCTGGACCAC ACATGTTTGG ATCAGCACAA 
CAGGCCAATC AGCCTAAGAC ACAACACATA AAGAACATAC CTAGTGGAAT GTGTCAAAAC 
CAATCGGGAC TTGCAGTGGC ACAAGGGCAG ATCCAACTGC ATGGGCAAGG ACATGCGCAG 
GGTCAGTCTT TAATTGGACC TACTAATAAT AATTTAATGT CAACTGCCGG AAGTGTCAGT 
GCTACTAACG GTGTCTCTGG CATCAATTTC GTAGGTCCCT CTTCTACGGA CCTGAAGTAT 
GCCCAGCAAT ATCATAGTTT TCAGCAGCAG TTATATGCTA CCAACACCAG AAGTCAACAA 
CAACAGCATA TGCACCAGCA GCACCAGAGC AACATGATAA CAATGCCGCC GAATTTATCA 
CCAAATCCAA CGTTCTTTGT CAACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651922.1
CDS	519..4928
Translation	MLSTTMPRSPTQQQPQPNSDASSTSASGSNPGAAIGNGDSAASRSSPKTLNSEPFSTLSPDQIKLTPEEGTEKSGLSTSDKAATGGAPGSGNNLPEGQTMLRQNSTSTINSCLVASPQNSSEHSNSSNVSATVGLTQMVDCDEQSKKNKCSVKDEEAEISSNKAKGQAAGGGCETGSTSSLTVKEEPTDVLGSLVNMKKEERENHSPTMSPVGFGSIGNAQDNSATPVKIERISNDSTTEKKGSSLTMNNDEMSMEGCNQLNPDFINESLNNPAISSILVSGVGPIPGIGVGAGTGNLLTANANGISSGSSNCLDYMQQQNHIFVFSTQLANKGAESVLSGQFQTIIAYHCTQPATKSFLEDFFMKNPLKINKLQRHNSVGMPWIGMGQVGLTPPNPVAKITQQQPHTKTVGLLKPQFNQHENSKRSTVSAPSNSFVDQSDPMGNETELMCWEGGSSNTSRSGQNSRNHVDSISTSSESQAIKILEAAGVDLGQVTKGSDPGLTTENNIVSLQGVKVPDENLTPQQRQHREEQLAKIKKMNQFLFPENENSVGANVSSQITKIPGDLMMGMSGGGGGSIINPTMRQLHMPGNAKSELLSATSSGLSEDVMHPGDVISDMGAVIGCNNNQKTSVQCGSGVGVVTGTTAAGVNVNMHCSSSGAPNGNMMGSSTDMLASFGNTSCNVIGTAPDMSKEVLNQDSRTHSHQGGVAQMEWSKIQHQFFEERLKGGKPRQVTGTVVPQQQTPSGSGGNSLNNQVRPLQGPPPPYHSIQRSASVPIATQSPNPSSPNNLSLPSPRTTAAVMGLPTNSPSMDGTGSLSGSVPQANTSTVQAGTTTVLSANKNCFQADTPSPSNQNRSRNTGSSSVLTHNLSSNPSTPLSHLSPKEFESFGQSSAGDNMKSRRPSPQGQRSPVNSLIEANKDVRFAASSPGFNPHPHMQSNSNSALNAYKMGSTNIQMERQASAQGGSVQFSRRSDNIPLNPNSGNRPPPNKMTQNFDPISSLAQMSQQLTSCVSSMGSPAGTGGMTMMGGPGPSDINIEHGIISGLDGSGIDTINQNNCHSMNVVMNSMGPRMLNPKMCVAGGPNGPPGFNPNSPNGGLRENSIGSGCGSANSSNFQGVVPPGARMMGRMPVNFGSNFNPNIQVKASTPNTIQYMPVRAQNANNNNNNGANNVRMPPSLEFLQRYANPQMGAVGNGSPICPPSASDGTPGMPGLMAGPGAGGMLMNSSGEQHQNKITNNPGASNGINFFQNCNQMSIVDEEGGLPGHDGSMNIGQPSMIRGMRPHAMRPNVMGARMPPVNRQIQFAQSSDGIDCVGDPSSFFTNASCNSAGPHMFGSAQQANQPKTQHIKNIPSGMCQNQSGLAVAQGQIQLHGQGHAQGQSLIGPTNNNLMSTAGSVSATNGVSGINFVGPSSTDLKYAQQYHSFQQQLYATNTRSQQQQHMHQQHQSNMITMPPNLSPNPTFFVNK

Target ORF information:

RefSeq Version	NM_143665.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster legless (lgs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143665.4

ORF Insert Sequence:

ATGCTCTCGA CAACAATGCC CCGCAGTCCA ACCCAACAAC AGCCGCAACC AAACTCCGAT 
GCCTCCTCAA CAAGTGCATC TGGATCAAAT CCTGGAGCAG CGATCGGAAA TGGGGACTCG 
GCGGCGAGCA GAAGTTCTCC GAAGACCCTT AATAGCGAAC CCTTTTCTAC TTTGTCGCCG 
GATCAAATAA AATTGACGCC AGAAGAAGGC ACTGAGAAAA GCGGACTATC AACTAGTGAT 
AAAGCTGCCA CTGGAGGAGC CCCAGGCAGT GGAAATAATC TGCCCGAGGG ACAAACTATG 
CTAAGGCAGA ACTCTACGAG CACAATCAAC TCGTGCCTAG TCGCTTCTCC ACAAAACTCC 
AGTGAACACT CGAATAGCAG CAATGTGTCT GCTACAGTGG GCCTTACTCA GATGGTAGAT 
TGTGACGAGC AATCGAAGAA AAACAAATGT AGTGTGAAGG ACGAGGAAGC TGAAATAAGT 
TCTAATAAAG CAAAAGGTCA AGCAGCTGGT GGCGGCTGCG AAACAGGTTC TACATCCAGT 
TTGACTGTCA AGGAAGAACC CACCGATGTC TTAGGCAGTT TAGTAAATAT GAAAAAAGAA 
GAAAGAGAAA ATCATTCGCC AACGATGTCC CCTGTTGGTT TTGGTTCAAT TGGTAATGCA 
CAGGACAACT CCGCTACACC GGTTAAAATT GAAAGAATTT CAAACGACAG TACCACGGAA 
AAAAAAGGAT CGTCCTTGAC AATGAATAAT GACGAAATGA GCATGGAAGG CTGCAATCAG 
TTGAATCCCG ATTTTATCAA TGAATCTTTA AATAATCCTG CAATTTCGAG CATATTAGTA 
AGCGGAGTAG GACCAATACC CGGAATCGGA GTTGGAGCGG GGACGGGAAA TTTATTGACT 
GCCAACGCCA ATGGAATCTC CTCGGGTAGC AGTAATTGTT TGGATTACAT GCAACAGCAA 
AATCACATAT TCGTGTTTTC AACTCAGCTG GCCAACAAAG GGGCCGAATC AGTTTTAAGC 
GGTCAATTTC AAACTATTAT TGCGTATCAC TGCACTCAGC CTGCTACAAA AAGCTTCCTG 
GAAGACTTTT TTATGAAAAA CCCTTTAAAG ATTAACAAGT TACAGCGGCA CAATTCCGTC 
GGTATGCCAT GGATAGGCAT GGGGCAGGTT GGACTAACTC CTCCTAATCC TGTAGCCAAA 
ATAACACAAC AGCAGCCACA TACAAAGACC GTAGGCCTAT TGAAACCCCA ATTCAATCAA 
CATGAAAACA GCAAACGTAG TACTGTAAGC GCGCCTAGCA ACTCTTTTGT CGACCAGTCT 
GATCCTATGG GCAACGAAAC TGAATTGATG TGCTGGGAAG GCGGATCCTC AAACACCAGT 
AGGTCTGGAC AAAACTCACG AAATCATGTA GACAGTATCA GTACATCCAG CGAGTCACAG 
GCAATAAAGA TACTGGAAGC AGCTGGCGTT GATTTGGGAC AGGTCACAAA AGGAAGCGAT 
CCTGGCCTGA CAACTGAAAA CAACATTGTA TCACTGCAAG GAGTTAAGGT TCCAGACGAA 
AACCTTACAC CACAACAGCG GCAACATCGG GAAGAACAGT TGGCAAAAAT AAAAAAAATG 
AATCAATTTC TTTTTCCTGA AAATGAGAAT TCAGTAGGAG CTAATGTAAG CTCACAGATA 
ACAAAAATTC CAGGAGATTT AATGATGGGG ATGTCGGGTG GCGGAGGCGG ATCTATTATA 
AATCCGACGA TGCGACAACT GCATATGCCA GGTAACGCCA AATCGGAGCT CTTATCGGCG 
ACAAGTTCAG GACTTTCGGA AGATGTAATG CATCCAGGGG ATGTTATATC AGATATGGGT 
GCCGTAATAG GATGTAATAA TAATCAAAAA ACCAGTGTGC AATGTGGATC TGGAGTAGGT 
GTTGTCACTG GAACAACTGC AGCTGGAGTA AATGTCAATA TGCATTGCTC AAGCTCCGGC 
GCCCCGAATG GCAATATGAT GGGAAGCTCT ACGGATATGC TAGCCTCGTT TGGCAACACA 
AGCTGCAACG TCATCGGAAC GGCCCCAGAT ATGTCTAAGG AAGTTTTAAA TCAAGATAGC 
CGAACCCATT CACATCAAGG GGGAGTTGCT CAAATGGAGT GGTCGAAGAT TCAACATCAA 
TTTTTCGAAG AACGCCTCAA GGGGGGCAAG CCCAGACAAG TCACTGGAAC TGTAGTACCA 
CAACAGCAAA CCCCTTCTGG ATCTGGTGGA AACTCGTTAA ACAACCAGGT GCGACCCCTG 
CAAGGTCCAC CTCCTCCTTA CCACTCCATC CAGAGATCTG CGTCAGTACC AATAGCCACT 
CAATCGCCCA ATCCCTCGAG TCCAAACAAT CTATCTCTCC CGTCACCGCG GACAACCGCA 
GCAGTCATGG GATTGCCGAC CAACTCTCCT AGCATGGATG GAACAGGATC ATTATCTGGA 
TCTGTTCCGC AAGCTAATAC TTCGACGGTT CAGGCAGGCA CAACAACAGT GCTCTCAGCA 
AACAAGAACT GTTTTCAGGC AGACACCCCA TCGCCGTCAA ATCAAAATCG TAGTAGAAAT 
ACCGGATCGT CAAGCGTTCT TACGCATAAC TTAAGCAGCA ACCCAAGTAC CCCCTTATCT 
CATCTATCCC CAAAGGAATT TGAGTCTTTC GGTCAGTCCT CTGCTGGTGA TAACATGAAA 
AGTAGGCGAC CAAGCCCACA GGGTCAGCGG TCACCAGTAA ATAGTCTAAT AGAGGCAAAT 
AAAGATGTAC GATTTGCTGC ATCCAGTCCT GGTTTTAACC CGCATCCACA TATGCAAAGC 
AATTCAAATT CAGCATTAAA CGCCTATAAA ATGGGCTCTA CCAATATACA GATGGAGCGT 
CAAGCATCAG CGCAAGGTGG ATCCGTACAA TTTAGTCGGC GCTCCGATAA TATTCCGCTA 
AATCCCAATA GTGGCAATCG GCCGCCACCA AACAAGATGA CCCAAAACTT CGATCCAATC 
TCTTCTTTGG CACAAATGTC CCAACAACTA ACAAGTTGCG TGTCCAGCAT GGGTAGTCCA 
GCCGGAACTG GTGGTATGAC GATGATGGGG GGTCCGGGAC CGTCCGACAT CAATATTGAG 
CATGGAATAA TTTCGGGACT AGATGGATCA GGAATAGATA CCATAAATCA AAATAACTGT 
CATTCAATGA ATGTCGTAAT GAACTCAATG GGTCCCCGAA TGCTGAATCC TAAAATGTGC 
GTAGCAGGCG GTCCAAATGG ACCGCCTGGC TTTAATCCTA ATTCCCCCAA TGGTGGATTA 
AGAGAGAATT CCATAGGGTC TGGCTGTGGC TCAGCAAACT CTTCAAACTT TCAAGGGGTT 
GTTCCACCTG GTGCCAGAAT GATGGGTCGA ATGCCAGTCA ATTTTGGTTC GAATTTCAAT 
CCGAATATTC AGGTAAAGGC GAGTACCCCA AACACCATAC AATACATGCC AGTAAGGGCA 
CAGAACGCCA ACAACAATAA CAACAATGGA GCTAATAATG TGCGAATGCC ACCTAGTCTG 
GAATTTTTGC AGAGGTACGC TAACCCTCAA ATGGGTGCTG TAGGCAATGG GTCGCCAATA 
TGCCCACCAT CAGCCAGCGA CGGTACTCCT GGAATGCCAG GATTGATGGC GGGACCAGGA 
GCCGGAGGTA TGCTAATGAA TTCTTCCGGA GAGCAACACC AGAACAAGAT CACAAACAAT 
CCTGGGGCAA GCAATGGTAT TAACTTCTTT CAGAATTGCA ATCAAATGTC TATTGTTGAC 
GAAGAGGGTG GATTACCCGG CCATGACGGA TCAATGAATA TTGGTCAACC ATCTATGATA 
AGGGGCATGC GTCCACATGC CATGCGGCCA AATGTAATGG GTGCGCGGAT GCCACCCGTT 
AACAGGCAAA TTCAGTTTGC ACAGTCATCG GATGGTATTG ACTGTGTCGG GGATCCGTCA 
TCATTTTTCA CTAACGCTTC CTGCAACAGC GCTGGACCAC ACATGTTTGG ATCAGCACAA 
CAGGCCAATC AGCCTAAGAC ACAACACATA AAGAACATAC CTAGTGGAAT GTGTCAAAAC 
CAATCGGGAC TTGCAGTGGC ACAAGGGCAG ATCCAACTGC ATGGGCAAGG ACATGCGCAG 
GGTCAGTCTT TAATTGGACC TACTAATAAT AATTTAATGT CAACTGCCGG AAGTGTCAGT 
GCTACTAACG GTGTCTCTGG CATCAATTTC GTAGGTCCCT CTTCTACGGA CCTGAAGTAT 
GCCCAGCAAT ATCATAGTTT TCAGCAGCAG TTATATGCTA CCAACACCAG AAGTCAACAA 
CAACAGCATA TGCACCAGCA GCACCAGAGC AACATGATAA CAATGCCGCC GAATTTATCA 
CCAAATCCAA CGTTCTTTGT CAACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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lgs cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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