RhoGAP100F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RhoGAP100F
Entrez Gene ID	43758
Full Name	Rho GTPase activating protein at 100F
Synonyms	1976,CG1976,DSyd-1,DSyd1,Dmel\CG1976,RHOGAP100F,Syd-1,dsyd-1,syd-1
General protein information	Preferred Names Rho GTPase activating protein at 100F Names CG1976 gene product from transcript CG1976-RD CG1976-PB CG1976-PC CG1976-PD CG1976-PE CG1976-PF CG1976-PH CG1976-PI CG1976-PJ RhoGAP100F-PB RhoGAP100F-PC RhoGAP100F-PD RhoGAP100F-PE RhoGAP100F-PF RhoGAP100F-PH RhoGAP100F-PI RhoGAP100F-PJ synpase defective-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100D2-100D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170314.2	NP_001163785.1	Rho GTPase activating protein at 100F, isoform B
NM_001300700.1	NP_001287629.1	Rho GTPase activating protein at 100F, isoform I
NM_001260468.2	NP_001247397.2	Rho GTPase activating protein at 100F, isoform J
NM_001170315.3	NP_001163786.1	Rho GTPase activating protein at 100F, isoform D
NM_001260466.1	NP_001247395.1	Rho GTPase activating protein at 100F, isoform E
NM_001260467.2	NP_001247396.1	Rho GTPase activating protein at 100F, isoform F
NM_001260465.1	NP_001247394.1	Rho GTPase activating protein at 100F, isoform H
NM_143645.2	NP_651902.2	Rho GTPase activating protein at 100F, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	RhoGAP100F	NP_001163785.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003944	XP_318402.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Syd-1/liprin-alpha/PP2A pathway stabilizes the active zone in synaptic terminals.
Title: Drosophila Syd-1, liprin-?, and protein phosphatase 2A B' subunit Wrd function in a linear pathway to prevent ectopic accumulation of synaptic materials in distal axons.

cooperation between Syd-1 and Nrx-1 seems to orchestrate early assembly processes between pre- and postsynaptic membranes
Title: Cooperation of Syd-1 with Neurexin synchronizes pre- with postsynaptic assembly.

DSyd-1 might stall synaptic proteins other than DLiprin-, e.g., adhesion molecules, to regulate postsynaptic maturation in a trans-synaptic manner.
Title: A Syd-1 homologue regulates pre- and postsynaptic maturation in Drosophila.

The following RhoGAP100F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RhoGAP100F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa22187	NM_001260468.2 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
OFa22186	NM_001300700.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
OFa07753	NM_001170314.2 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa07755	NM_001260466.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa06813	NM_143645.2 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa07752	NM_001170315.3 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa07754	NM_001260465.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa07756	NM_001260467.2 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RhoGAP100F ( NM_001260468.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247397.2 Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant J, mRNA.

Price & Availability↑

CloneID	OFa22187
Clone ID Related Accession (Same CDS sequence)	NM_001260468.2
Accession Version	NM_001260468.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 100F, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260468.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTGGA AGAAGAAGTT TACTCGATTG AAAGCAGCTA CTGGAAATTC GCGTGTGCGA 
AGAATGCTTT GTTGTGGACG CAGAAAGGAA AATGGAAGAT CAGTTCCTGA TGTCACCGCA 
AGCCCGGGAC GGGCCCCGCC CGGACCACTG CCCGCCAACC AGATGCCTGC GATGGGCAAC 
CAGCAACACC ATGGAAACCA GCAACATCAT GGAAACCAGC AGCAACATCA TGGCAATCAG 
CACAGCAACC ATCGAGGTCA GAGCGGAAGT TTGTCAAACG CCGCCGGGGT CAAGGACCCG 
GTGATGCTAC AGGGCGATTT TCGGAAAGTC AGCGGAATCA GCTCAGAGAT CTTTCGGCAG 
ATAGAAGCCG TCGAGAATGA CCACGACCCA AACACGGCGG CGGCTTTGGA GGCGGTGGAG 
AGACGCGGTG AGATGATCGT GCGCGTCCTG GAGCCGCGTT GCATGGGCAG CAAACAGGCG 
GTGGACGCTG CCCACAAGCT GATGAACAAG GCGGACGCAC GGCACACCGT GCAGCTGGTA 
GAAATAGTCA AGCGGCCGGG CCAGACGCTG GGCTTGTACA TCCGCGAGGG AAATGGGGCC 
GATCGAACTG ATGGCGTCTT TATTTCGCGG ATTGCACTGG AGTCGGCTGT CTACAACAGC 
GGCTGCTTGC GGGTGGGCGA CGAAATCCTT GCGGTAAATC TGGTGGACGT GACACACATG 
TCCTTAGACG ATGTCGTCAT TATTATGTCA ATTCCGCGCC GCTTGGTGCT GGCTATACGT 
CAGCGGCGGG GCAATAGAGG CACAGGATCT CCTGGACCGC CAACGCTCTC TAGGCCAGAA 
CAGAAGCCCC CACCGGTTGT TGTCATCAAG CGAGATTTGC GAGACGAGGA TCTGGACGAG 
ACGGACCGGA TGCCGAGGCC GCGCTCATCA CGTGACAGAC GTACAGGAGA TGGTCGCGAA 
ATGACGGAAT CTCGTTCTAG GCTAGGTCTG GGTCTTAATA ATTACAGCCC GCAGTCCGAG 
CAGTTGGATA TGTACTACAA TACCCGCGGC GGAGGTGGAG GAGCCATGGG TGAGCCTCCA 
AACTGGGGCT ATAAACCACC ACCGCCACCG TCTTCGGTCA TTACGGAGCA ACCCACGAAA 
GCACATGCTT TTGCGCCGTC GCATGCTTAC TACCAAAATG CCGGCACTCT TGAAAGCTTG 
GCGGAGAAGG TACATGCATT CTATCCTGGA CAGCCTGGTG GTCCGCCAGT GGGTCCCTCG 
AGAAGAATGT CAACGGGAAC TGGAAACGTT GGCCTCGCTC AGCAACATGC CAGATTTCCG 
CGATCTGGCT CAGATCAGCA TTTACCTCGC GTAGAATATT CGGACTATTC CAATTCACTG 
GGGCGGCATT CCCTTCTGCG ATCAAGTTTA AAACCTGGAA CGACCGGTGG AGCTCCAATG 
CAGGTAGGAG TGGGAGGCAC TCTTGGCCGA TACGGCCGCT ATGATCAACA GAGAGCCGGT 
GTATCCAAGT ACGGCCCTCC ATCTGGTGGA GCTCAATCGC TGACCCGTCG TTCCAGACCA 
AATTTGGACT ATTCCAGCGA TACGGAAGCC ACAATTGGGC CCAGGCCAAG TTACTACTAC 
TATAACAGAC CTGCCATCGG CAGTATGTCG AGGGGATCAG GTGGAGCAGG CGGAGGAGTT 
GGCGCAGCTT CAACGGCTGC CTTGCTGGCT GGAGCTGCTG ATCTTAACAA ATTTAACTCG 
TTGCCACGAG AGCGCCCCGG GACGAGACTG CAGGGAATTC GTTCCAGAAT GGGAGATCGT 
TTGGTGGACG AAAATGACGG AAATACTTCG GCACCCGAGT TCGATGTACG GAGAGGAAGG 
GACTTACGTC AGCGAATTAC CGCCAGTCCG TCGATATTTA CGGCAGATGA ATACCGCGCC 
TGGCTCAGAA GGGCGCCTAG CAGTTCCGCA ATTGCGGAAC AAATGCGAAT GACGCGGGAC 
ATGTTTGCCC AGCCACGGGC TCAGCGATTT TCGTGTAGCG CTGAGAACAT CCATGATGCA 
CTGAGAAATA CGGAAAGCAT CTACTCCAGT AGAAACCATA TTCTTGGTAC GGGCACTCTC 
GATCGGAACA TGGGTCTTAC CCGACCAATT TCAGCACTAC CCGTGCGCTC CATGTCCTCA 
CAGCACATTG GAGGAGCGGG TTCCATTCGT TCGCCTAGCA TACGGCGCAT GCGACAGTTA 
CTGGAGCTTT CCGCTGGTCC CGCCAGTCCG AGCGGCAGTA TCTTGAGCAC CGGTGGTCAT 
CAGAGTCCGG CACCAACTCC AAGCGCGACC CTACCACGCC CGCACCGCCA AATCGACATC 
AACCCGGCGG AGTTTGCCAA GTACAAGCTG GATAAGCCCA TCGTTGATAT TGGGGGGATC 
TCCGGCATGT TATGGATTCA TTTGCTAGCA GGTCGCGGCC TAAGAACCGC TCCAGAGGGA 
GCAGCAGGAA CGGCGACACA GGGCCAAACC AGAGATCTTT ACTGCGTAAT CGAGTGCGAT 
CGAGTACATA AAGCACGGAC TGTGGTACGA TCGGGTGACC TGCAGTTCGA CTGGGACGAG 
TCGTTTGAGC TCGACTTGGT GGGCAACAAA CAGTTAGATG TACTAGTCTA CTCATGGGAC 
CCGCAGCACA GACACAAGCT GTGTTACCGA GGCGCAATCT CGTTATCGTC GATCCTCCGT 
CAGTCTCCAC TTCATCAACT GGCCTTGAAG GTTGAACCAA GAGGTACGAT ATACATTCGC 
ATGCGGCACA CGGATCCACT GGCTCTCTAC AAGAGAAGGG GACTTCCGAG CCTGAGAGCA 
GGTTACCCTA CGCTCTTCGG CGCTGATTTG GAAACGGTGG TGAATCGGGA GTCTAAGAAT 
GCTCCCGGAA GTGCACCTGT TCCCATCGTA TTAAGGCGCT GTGTGGAGGA GGTGGAACGG 
CGTGGTCTTG ATATAATCGG GTTGTACCGC CTGTGCGGCT CGGCTACCAA GAAGCGATTG 
CTACGCGAGG CCTTTGAGCG CAATAGCCGT GCAGTGGAAT TGACCCCGGA ACATGTTCCT 
GACATCAACG TCATCACCGG CGTCCTCAAG GATTACCTTA GGGAGCTGCC CGAGCCGCTG 
TTCACGCGCT GTCTTTTCCA GATGACGGTG GATGCCTTAG CTGTCTGCCT GCCAGATGAC 
CCAGAGGGCA ATGCGAAACT GATGCTTAGC ATACTCGACT GCCTACCGCG GGCGAACAGG 
GCCACCCTGG TATTCCTTCT TGATCACCTC TCGCTGGTCG TTTCAAACTC GGAGCGGAAC 
AAGATGTCAG CCCAGGCGTT GGCCACCGTG ATGGGCCCAC CGCTGATGCT GCATTCGGCG 
AGTGCGCAGC CGGGTGCTGA CATCGATCAC GCTCAGCCGA TCGCGGTGCT TAAGTATCTT 
CTGCAGATCT GGCCGCAGCC GCAGGCGCAG CATCAGCAGA TGGCGCAGCA CATGGGCGGC 
GCTGCGGGGG CAATGATGGG CGGTTTGGTC ACCGCCGGGA GCATGAGCAA TATGGCCGGT 
GTTGCTTCAG ACAGGTCGGC GCGGCGAGTC AACAGGGCAG CGTGGAAGCA AAGTCAGTGC 
GTTGCCAGCG GACAGACAGC AACTTCTACT GCAGCAGCAG GCGCAGCTCA TGGCGGCGGG 
CAATCTTCTG CGCTCGTCCA CTTCGGTAAC CAACATACTC TCCCAAGGCC ATCCTCAGCT 
CTCAGCCACA GCCAACAATC ATCTGTATCA ATCAGTAGTG GGTCAGTTAG CTCAATCGCA 
TCGAGCCTTG CAACAAGCGG TGCAACAGCC CTATCAATTG GGGGGCTCAG TGGGCTCAGC 
AATTCCCGAC CCATCGCCAC TGCCACTTCC AGGCACACCC TCCCCCGGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247397.2
CDS	1432..5325
Translation	MQWKKKFTRLKAATGNSRVRRMLCCGRRKENGRSVPDVTASPGRAPPGPLPANQMPAMGNQQHHGNQQHHGNQQQHHGNQHSNHRGQSGSLSNAAGVKDPVMLQGDFRKVSGISSEIFRQIEAVENDHDPNTAAALEAVERRGEMIVRVLEPRCMGSKQAVDAAHKLMNKADARHTVQLVEIVKRPGQTLGLYIREGNGADRTDGVFISRIALESAVYNSGCLRVGDEILAVNLVDVTHMSLDDVVIIMSIPRRLVLAIRQRRGNRGTGSPGPPTLSRPEQKPPPVVVIKRDLRDEDLDETDRMPRPRSSRDRRTGDGREMTESRSRLGLGLNNYSPQSEQLDMYYNTRGGGGGAMGEPPNWGYKPPPPPSSVITEQPTKAHAFAPSHAYYQNAGTLESLAEKVHAFYPGQPGGPPVGPSRRMSTGTGNVGLAQQHARFPRSGSDQHLPRVEYSDYSNSLGRHSLLRSSLKPGTTGGAPMQVGVGGTLGRYGRYDQQRAGVSKYGPPSGGAQSLTRRSRPNLDYSSDTEATIGPRPSYYYYNRPAIGSMSRGSGGAGGGVGAASTAALLAGAADLNKFNSLPRERPGTRLQGIRSRMGDRLVDENDGNTSAPEFDVRRGRDLRQRITASPSIFTADEYRAWLRRAPSSSAIAEQMRMTRDMFAQPRAQRFSCSAENIHDALRNTESIYSSRNHILGTGTLDRNMGLTRPISALPVRSMSSQHIGGAGSIRSPSIRRMRQLLELSAGPASPSGSILSTGGHQSPAPTPSATLPRPHRQIDINPAEFAKYKLDKPIVDIGGISGMLWIHLLAGRGLRTAPEGAAGTATQGQTRDLYCVIECDRVHKARTVVRSGDLQFDWDESFELDLVGNKQLDVLVYSWDPQHRHKLCYRGAISLSSILRQSPLHQLALKVEPRGTIYIRMRHTDPLALYKRRGLPSLRAGYPTLFGADLETVVNRESKNAPGSAPVPIVLRRCVEEVERRGLDIIGLYRLCGSATKKRLLREAFERNSRAVELTPEHVPDINVITGVLKDYLRELPEPLFTRCLFQMTVDALAVCLPDDPEGNAKLMLSILDCLPRANRATLVFLLDHLSLVVSNSERNKMSAQALATVMGPPLMLHSASAQPGADIDHAQPIAVLKYLLQIWPQPQAQHQQMAQHMGGAAGAMMGGLVTAGSMSNMAGVASDRSARRVNRAAWKQSQCVASGQTATSTAAAGAAHGGGQSSALVHFGNQHTLPRPSSALSHSQQSSVSISSGSVSSIASSLATSGATALSIGGLSGLSNSRPIATATSRHTLPRQ

Target ORF information:

RefSeq Version	NM_001260468.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260468.2

ORF Insert Sequence:

ATGCAGTGGA AGAAGAAGTT TACTCGATTG AAAGCAGCTA CTGGAAATTC GCGTGTGCGA 
AGAATGCTTT GTTGTGGACG CAGAAAGGAA AATGGAAGAT CAGTTCCTGA TGTCACCGCA 
AGCCCGGGAC GGGCCCCGCC CGGACCACTG CCCGCCAACC AGATGCCTGC GATGGGCAAC 
CAGCAACACC ATGGAAACCA GCAACATCAT GGAAACCAGC AGCAACATCA TGGCAATCAG 
CACAGCAACC ATCGAGGTCA GAGCGGAAGT TTGTCAAACG CCGCCGGGGT CAAGGACCCG 
GTGATGCTAC AGGGCGATTT TCGGAAAGTC AGCGGAATCA GCTCAGAGAT CTTTCGGCAG 
ATAGAAGCCG TCGAGAATGA CCACGACCCA AACACGGCGG CGGCTTTGGA GGCGGTGGAG 
AGACGCGGTG AGATGATCGT GCGCGTCCTG GAGCCGCGTT GCATGGGCAG CAAACAGGCG 
GTGGACGCTG CCCACAAGCT GATGAACAAG GCGGACGCAC GGCACACCGT GCAGCTGGTA 
GAAATAGTCA AGCGGCCGGG CCAGACGCTG GGCTTGTACA TCCGCGAGGG AAATGGGGCC 
GATCGAACTG ATGGCGTCTT TATTTCGCGG ATTGCACTGG AGTCGGCTGT CTACAACAGC 
GGCTGCTTGC GGGTGGGCGA CGAAATCCTT GCGGTAAATC TGGTGGACGT GACACACATG 
TCCTTAGACG ATGTCGTCAT TATTATGTCA ATTCCGCGCC GCTTGGTGCT GGCTATACGT 
CAGCGGCGGG GCAATAGAGG CACAGGATCT CCTGGACCGC CAACGCTCTC TAGGCCAGAA 
CAGAAGCCCC CACCGGTTGT TGTCATCAAG CGAGATTTGC GAGACGAGGA TCTGGACGAG 
ACGGACCGGA TGCCGAGGCC GCGCTCATCA CGTGACAGAC GTACAGGAGA TGGTCGCGAA 
ATGACGGAAT CTCGTTCTAG GCTAGGTCTG GGTCTTAATA ATTACAGCCC GCAGTCCGAG 
CAGTTGGATA TGTACTACAA TACCCGCGGC GGAGGTGGAG GAGCCATGGG TGAGCCTCCA 
AACTGGGGCT ATAAACCACC ACCGCCACCG TCTTCGGTCA TTACGGAGCA ACCCACGAAA 
GCACATGCTT TTGCGCCGTC GCATGCTTAC TACCAAAATG CCGGCACTCT TGAAAGCTTG 
GCGGAGAAGG TACATGCATT CTATCCTGGA CAGCCTGGTG GTCCGCCAGT GGGTCCCTCG 
AGAAGAATGT CAACGGGAAC TGGAAACGTT GGCCTCGCTC AGCAACATGC CAGATTTCCG 
CGATCTGGCT CAGATCAGCA TTTACCTCGC GTAGAATATT CGGACTATTC CAATTCACTG 
GGGCGGCATT CCCTTCTGCG ATCAAGTTTA AAACCTGGAA CGACCGGTGG AGCTCCAATG 
CAGGTAGGAG TGGGAGGCAC TCTTGGCCGA TACGGCCGCT ATGATCAACA GAGAGCCGGT 
GTATCCAAGT ACGGCCCTCC ATCTGGTGGA GCTCAATCGC TGACCCGTCG TTCCAGACCA 
AATTTGGACT ATTCCAGCGA TACGGAAGCC ACAATTGGGC CCAGGCCAAG TTACTACTAC 
TATAACAGAC CTGCCATCGG CAGTATGTCG AGGGGATCAG GTGGAGCAGG CGGAGGAGTT 
GGCGCAGCTT CAACGGCTGC CTTGCTGGCT GGAGCTGCTG ATCTTAACAA ATTTAACTCG 
TTGCCACGAG AGCGCCCCGG GACGAGACTG CAGGGAATTC GTTCCAGAAT GGGAGATCGT 
TTGGTGGACG AAAATGACGG AAATACTTCG GCACCCGAGT TCGATGTACG GAGAGGAAGG 
GACTTACGTC AGCGAATTAC CGCCAGTCCG TCGATATTTA CGGCAGATGA ATACCGCGCC 
TGGCTCAGAA GGGCGCCTAG CAGTTCCGCA ATTGCGGAAC AAATGCGAAT GACGCGGGAC 
ATGTTTGCCC AGCCACGGGC TCAGCGATTT TCGTGTAGCG CTGAGAACAT CCATGATGCA 
CTGAGAAATA CGGAAAGCAT CTACTCCAGT AGAAACCATA TTCTTGGTAC GGGCACTCTC 
GATCGGAACA TGGGTCTTAC CCGACCAATT TCAGCACTAC CCGTGCGCTC CATGTCCTCA 
CAGCACATTG GAGGAGCGGG TTCCATTCGT TCGCCTAGCA TACGGCGCAT GCGACAGTTA 
CTGGAGCTTT CCGCTGGTCC CGCCAGTCCG AGCGGCAGTA TCTTGAGCAC CGGTGGTCAT 
CAGAGTCCGG CACCAACTCC AAGCGCGACC CTACCACGCC CGCACCGCCA AATCGACATC 
AACCCGGCGG AGTTTGCCAA GTACAAGCTG GATAAGCCCA TCGTTGATAT TGGGGGGATC 
TCCGGCATGT TATGGATTCA TTTGCTAGCA GGTCGCGGCC TAAGAACCGC TCCAGAGGGA 
GCAGCAGGAA CGGCGACACA GGGCCAAACC AGAGATCTTT ACTGCGTAAT CGAGTGCGAT 
CGAGTACATA AAGCACGGAC TGTGGTACGA TCGGGTGACC TGCAGTTCGA CTGGGACGAG 
TCGTTTGAGC TCGACTTGGT GGGCAACAAA CAGTTAGATG TACTAGTCTA CTCATGGGAC 
CCGCAGCACA GACACAAGCT GTGTTACCGA GGCGCAATCT CGTTATCGTC GATCCTCCGT 
CAGTCTCCAC TTCATCAACT GGCCTTGAAG GTTGAACCAA GAGGTACGAT ATACATTCGC 
ATGCGGCACA CGGATCCACT GGCTCTCTAC AAGAGAAGGG GACTTCCGAG CCTGAGAGCA 
GGTTACCCTA CGCTCTTCGG CGCTGATTTG GAAACGGTGG TGAATCGGGA GTCTAAGAAT 
GCTCCCGGAA GTGCACCTGT TCCCATCGTA TTAAGGCGCT GTGTGGAGGA GGTGGAACGG 
CGTGGTCTTG ATATAATCGG GTTGTACCGC CTGTGCGGCT CGGCTACCAA GAAGCGATTG 
CTACGCGAGG CCTTTGAGCG CAATAGCCGT GCAGTGGAAT TGACCCCGGA ACATGTTCCT 
GACATCAACG TCATCACCGG CGTCCTCAAG GATTACCTTA GGGAGCTGCC CGAGCCGCTG 
TTCACGCGCT GTCTTTTCCA GATGACGGTG GATGCCTTAG CTGTCTGCCT GCCAGATGAC 
CCAGAGGGCA ATGCGAAACT GATGCTTAGC ATACTCGACT GCCTACCGCG GGCGAACAGG 
GCCACCCTGG TATTCCTTCT TGATCACCTC TCGCTGGTCG TTTCAAACTC GGAGCGGAAC 
AAGATGTCAG CCCAGGCGTT GGCCACCGTG ATGGGCCCAC CGCTGATGCT GCATTCGGCG 
AGTGCGCAGC CGGGTGCTGA CATCGATCAC GCTCAGCCGA TCGCGGTGCT TAAGTATCTT 
CTGCAGATCT GGCCGCAGCC GCAGGCGCAG CATCAGCAGA TGGCGCAGCA CATGGGCGGC 
GCTGCGGGGG CAATGATGGG CGGTTTGGTC ACCGCCGGGA GCATGAGCAA TATGGCCGGT 
GTTGCTTCAG ACAGGTCGGC GCGGCGAGTC AACAGGGCAG CGTGGAAGCA AAGTCAGTGC 
GTTGCCAGCG GACAGACAGC AACTTCTACT GCAGCAGCAG GCGCAGCTCA TGGCGGCGGG 
CAATCTTCTG CGCTCGTCCA CTTCGGTAAC CAACATACTC TCCCAAGGCC ATCCTCAGCT 
CTCAGCCACA GCCAACAATC ATCTGTATCA ATCAGTAGTG GGTCAGTTAG CTCAATCGCA 
TCGAGCCTTG CAACAAGCGG TGCAACAGCC CTATCAATTG GGGGGCTCAG TGGGCTCAGC 
AATTCCCGAC CCATCGCCAC TGCCACTTCC AGGCACACCC TCCCCCGGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP100F ( NM_001300700.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287629.1 Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa22186
Clone ID Related Accession (Same CDS sequence)	NM_001300700.1
Accession Version	NM_001300700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 100F, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTGGA AGAAGAAGTT TACTCGATTG AAAGCAGCTA CTGGAAATTC GCGTGTGCGA 
AGAATGCTTT GTTGTGGACG CAGAAAGGAA AATGGAAGAT CAGTTCCTGA TGTCACCGCA 
AGCCCGGGAC GGGCCCCGCC CGGACCACTG CCCGCCAACC AGATGCCTGC GATGGGCAAC 
CAGCAACACC ATGGAAACCA GCAACATCAT GGAAACCAGC AGCAACATCA TGGCAATCAG 
CACAGCAACC ATCGAGGTCA GAGCGGAAGT TTGTCAAACG CCGCCGGGGT CAAGGACCCG 
GTGATGCTAC AGGGCGATTT TCGGAAAGTC AGCGGAATCA GCTCAGAGAT CTTTCGGCAG 
ATAGAAGCCG TCGAGAATGA CCACGACCCA AACACGGCGG CGGCTTTGGA GGCGGTGGAG 
AGACGCGGTG AGATGATCGT GCGCGTCCTG GAGCCGCGTT GCATGGGCAG CAAACAGGCG 
GTGGACGCTG CCCACAAGCT GATGAACAAG GCGGACGCAC GGCACACCGT GCAGCTGGTA 
GAAATAGTCA AGCGGCCGGG CCAGACGCTG GGCTTGTACA TCCGCGAGGG AAATGGGGCC 
GATCGAACTG ATGGCGTCTT TATTTCGCGG ATTGCACTGG AGTCGGCTGT CTACAACAGC 
GGCTGCTTGC GGGTGGGCGA CGAAATCCTT GCGGTAAATC TGGTGGACGT GACACACATG 
TCCTTAGACG ATGTCGTCAT TATTATGTCA ATTCCGCGCC GCTTGGTGCT GGCTATACGT 
CAGCGGCGGG GCAATAGAGG CACAGGATCT CCTGGACCGC CAACGCTCTC TAGGCCAGAA 
CAGAAGCCCC CACCGGTTGT TGTCATCAAG CGAGATTTGC GAGACGAGGA TCTGGACGAG 
ACGGACCGGA TGCCGAGGCC GCGCTCATCA CGTGACAGAC GTACAGGAGA TGGTCGCGAA 
ATGACGGAAT CTCGTTCTAG GCTAGGTCTG GGTCTTAATA ATTACAGCCC GCAGTCCGAG 
CAGTTGGATA TGTACTACAA TACCCGCGGC GGAGGTGGAG GAGCCATGGG TGAGCCTCCA 
AACTGGGGCT ATAAACCACC ACCGCCACCG TCTTCGGTCA TTACGGAGCA ACCCACGAAA 
GCACATGCTT TTGCGCCGTC GCATGCTTAC TACCAAAATG CCGGCACTCT TGAAAGCTTG 
GCGGAGAAGG TACATGCATT CTATCCTGGA CAGCCTGGTG GTCCGCCAGT GGGTCCCTCG 
AGAAGAATGT CAACGGGAAC TGGAAACGTT GGCCTCGCTC AGCAACATGC CAGATTTCCG 
CGATCTGGCT CAGATCAGCA TTTACCTCGC GTAGAATATT CGGACTATTC CAATTCACTG 
GGGCGGCATT CCCTTCTGCG ATCAAGTTTA AAACCTGGAA CGACCGGTGG AGCTCCAATG 
CAGGTAGGAG TGGGAGGCAC TCTTGGCCGA TACGGCCGCT ATGATCAACA GAGAGCCGGT 
GTATCCAAGT ACGGCCCTCC ATCTGGTGGA GCTCAATCGC TGACCCGTCG TTCCAGACCA 
AATTTGGACT ATTCCAGCGA TACGGAAGCC ACAATTGGGC CCAGGCCAAG TTACTACTAC 
TATAACAGAC CTGCCATCGG CAGTATGTCG AGGGGATCAG GTGGAGCAGG CGGAGGAGTT 
GGCGCAGCTT CAACGGCTGC CTTGCTGGCT GGAGCTGCTG ATCTTAACAA ATTTAACTCG 
TTGCCACGAG AGCGCCCCGG GACGAGACTG CAGGGAATTC GTTCCAGAAT GGGAGATCGT 
TTGGTGGACG AAAATGACGG AAATACTTCG GCACCCGAGT TCGATGTACG GAGAGGAAGG 
GACTTACGTC AGCGAATTAC CGCCAGTCCG TCGATATTTA CGGCAGATGA ATACCGCGCC 
TGGCTCAGAA GGGCGCCTAG CAGTTCCGCA ATTGCGGAAC AAATGCGAAT GACGCGGGAC 
ATGTTTGCCC AGCCACGGGC TCAGCGATTT TCGTGTAGCG CTGAGAACAT CCATGATGCA 
CTGAGAAATA CGGAAAGCAT CTACTCCAGT AGAAACCATA TTCTTGGTAC GGGCACTCTC 
GATCGGAACA TGGGTCTTAC CCGACCAATT TCAGCACTAC CCGTGCGCTC CATGTCCTCA 
CAGCACATTG GAGGAGCGGG TTCCATTCGT TCGCCTAGCA TACGGCGCAT GCGACAGTTA 
CTGGAGCTTT CCGCTGGTCC CGCCAGTCCG AGCGGCAGTA TCTTGAGCAC CGGTGGTCAT 
CAGAGTCCGG CACCAACTCC AAGCGCGACC CTACCACGCC CGCACCGCCA AATCGACATC 
AACCCGGCGG AGTTTGCCAA GTACAAGCTG GATAAGCCCA TCGTTGATAT TGGGGGGATC 
TCCGGCATGT TATGGATTCA TTTGCTAGCA GGTCGCGGCC TAAGAACCGC TCCAGAGGGA 
GCAGCAGGAA CGGCGACACA GGGCCAAACC AGAGATCTTT ACTGCGTAAT CGAGTGCGAT 
CGAGTACATA AAGCACGGAC TGTGGTACGA TCGGGTGACC TGCAGTTCGA CTGGGACGAG 
TCGTTTGAGC TCGACTTGGT GGGCAACAAA CAGTTAGATG TACTAGTCTA CTCATGGGAC 
CCGCAGCACA GACACAAGCT GTGTTACCGA GGCGCAATCT CGTTATCGTC GATCCTCCGT 
CAGTCTCCAC TTCATCAACT GGCCTTGAAG GTTGAACCAA GAGGTACGAT ATACATTCGC 
ATGCGGCACA CGGATCCACT GGCTCTCTAC AAGAGAAGGG GACTTCCGAG CCTGAGAGCA 
GGTTACCCTA CGCTCTTCGG CGCTGATTTG GAAACGGTGG TGAATCGGGA GTCTAAGAAT 
GCTCCCGGAA GTGCACCTGT TCCCATCGTA TTAAGGCGCT GTGTGGAGGA GGTGGAACGG 
CGTGGTCTTG ATATAATCGG GTTGTACCGC CTGTGCGGCT CGGCTACCAA GAAGCGATTG 
CTACGCGAGG CCTTTGAGCG CAATAGCCGT GCAGTGGAAT TGACCCCGGA ACATGTTCCT 
GACATCAACG TCATCACCGG CGTCCTCAAG GATTACCTTA GGGAGCTGCC CGAGCCGCTG 
TTCACGCGCT GTCTTTTCCA GATGACGGTG GATGCCTTAG CTGTCTGCCT GCCAGATGAC 
CCAGAGGGCA ATGCGAAACT GATGCTTAGC ATACTCGACT GCCTACCGCG GGCGAACAGG 
GCCACCCTGG TATTCCTTCT TGATCACCTC TCGCTGGTCG TTTCAAACTC GGAGCGGAAC 
AAGATGTCAG CCCAGGCGTT GGCCACCGTG ATGGGCCCAC CGCTGATGCT GCATTCGGCG 
AGTGCGCAGC CGGGTGCTGA CATCGATCAC GCTCAGCCGA TCGCGGTGCT TAAGTATCTT 
CTGCAGATCT GGCCGCAGCC GCAGGCGCAG CATCAGCAGA TGGCGCAGCA CATGGGCGGC 
GCTGCGGGGG CAATGATGGG CGGTTTGGTC ACCGCCGGGA GCATGAGCAA TATGGCCGGT 
GTTGCTTCAG GTCGGCGCGG CGAGTCAACA GGGCAGCGTG GAAGCAAAGT CAGTGCGTTG 
CCAGCGGACA GACAGCAACT TCTACTGCAG CAGCAGGCGC AGCTCATGGC GGCGGGCAAT 
CTTCTGCGCT CGTCCACTTC GCCCTATCAA TTGGGGGGCT CAGTGGGCTC AGCAATTCCC 
GACCCATCGC CACTGCCACT TCCAGGCACA CCCTCCCCCG GCAGTAGCTC AGCATCGACG 
GGCTCAGGCT CCGGATCGGG TAAAAGCACG GACACCATCA AGCGCGGTGC TTCGCCTGTT 
TCCGTTAAGC AAGTCAAGAT CGTCGACCAG CCATCCAGCC CATACTCCAT TGTGATGAAG 
AAACCGCCGT TGCAAAAAGA TGCGCCCGTT GAAATCACCA CCCCCACCAC ACAGGCTGAT 
ACAGAGTCGA CCTTGGGATG CAAGGAAAGC AATGGAACAG CTTCTCGAAG GGGAAATGTA 
GACTTTTATG ATACGCATAA GACGCAGGCA AAGAGTGTGG TAAACGAGGA ATCCAGCTAC 
AGCTCCAAGT ACACTGGCTC GGAGACCAAG AAGATCATCC CTGGCAACAG TAGCTACACT 
CCCAGCAAGG CGAATGCTAG TGGCCTTTCT GGCGGCGAAG ACTACAAAGC AATGCGTAAC 
AAGTCGAGCG CTACATCCAG CTCCAGTTCA TCGCAGGCAA CCGTTTTGAG TGCTGGCTCT 
ACCGCCACAT CAGCCCCGAC CACTTCTTCA GACGATTCGG ATGACCTGGT CTCCTACAAG 
TCATCGGCGT CCACAAATGC CTTGCTGGCC CAATCGCAGG CCATGACCAC CAGCCAGCTG 
ATGTCCAAGT ATCTTAAGCG GGAGCCACGA GTACAGTTTA CGCCAATCAA GTCACCGGAA 
TCTCCCTCGC CACCGGGTAG CGGCGATGGT CTGCCGAAGG GTACTTACCA ACTAGTTACG 
CCTATCTCGG GATCGTCGAG CAAACCGGGT GCTACAACTG GAGCTATAAG CAAGTATACC 
ACCGGCTCAG TGGAATCATC TATTAATGCG AACAGCCAGA AGTTGTCATC GCCCTCACGA 
TTGTGCAACA GCAAGGATAG CAATAGCAGG ACTGGAACGG CCAGTAGTAC GACCCCCGCG 
ACCTCCATGG TTTCTACAGG TCGGCGTTTG TTTGACAGTC TCGCCTCTTC ATCGTCCTCG 
GAAACGGAGA CCAAGACGTA CATAGGTGGC ACCACTGCAG CCAGTGGAGC CATTACCACC 
ACCATCTACA CCAACGACAC CAAGAACTCC GGCAGCAGTA GCAGTAAGTC GGGAATAGGA 
GGAGGGTCTG GAACTGGGTT GGGAGCAGTT TCGGGAGCAA GCTCCGAGAC CCGAAGCTTC 
GGCAGTACGC TATTTGGCAG TAGTGGCTTA GGTAATGGCA ATGGTAGTAG CCACAACCAC 
TCTAGCGCCA GTCCAAGTCC CTTCACCACC ACCAACGGCA ACGGTAACCA CAATACCATG 
CATCTGTACG GCACCTTGCC CAAAAACGGA ACTTCCACGG GGGCAGCTCT GTTTGGCGGA 
TCGGCCAACA GTTCTTCGTA CCACTCTTCG GCGAGTGGAA GTGGAGCCGG AACCGCCAGC 
AGTAGTGGGG TCAGCTCTAT GACTGGCTCC ACAAACAGCT ACGACTTCTA CACGAGCACA 
AGTTCCACTG TGAGCAGTTC GCGCCCCTTT GCCAATGGAG GCAACAACTA TCACACACTG 
GGAACCTATA GGGCTCAGTA CGCCGCCACT AACCCCTTCC TGGACGCATT TGATGAAAAG 
CCGGGCAGCA ACGGGGGTAA TGCCCACGGG GAGGAGAAGC TAGGCGCGGA CAAGGGCCAC 
CACAGAGCGG CCGTTATGGC CTTTCAGTCT TCAGGAGACT CGAAAAACGG TAGCGATGAG 
TACGATGACA TCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287629.1
CDS	1432..6909
Translation	MQWKKKFTRLKAATGNSRVRRMLCCGRRKENGRSVPDVTASPGRAPPGPLPANQMPAMGNQQHHGNQQHHGNQQQHHGNQHSNHRGQSGSLSNAAGVKDPVMLQGDFRKVSGISSEIFRQIEAVENDHDPNTAAALEAVERRGEMIVRVLEPRCMGSKQAVDAAHKLMNKADARHTVQLVEIVKRPGQTLGLYIREGNGADRTDGVFISRIALESAVYNSGCLRVGDEILAVNLVDVTHMSLDDVVIIMSIPRRLVLAIRQRRGNRGTGSPGPPTLSRPEQKPPPVVVIKRDLRDEDLDETDRMPRPRSSRDRRTGDGREMTESRSRLGLGLNNYSPQSEQLDMYYNTRGGGGGAMGEPPNWGYKPPPPPSSVITEQPTKAHAFAPSHAYYQNAGTLESLAEKVHAFYPGQPGGPPVGPSRRMSTGTGNVGLAQQHARFPRSGSDQHLPRVEYSDYSNSLGRHSLLRSSLKPGTTGGAPMQVGVGGTLGRYGRYDQQRAGVSKYGPPSGGAQSLTRRSRPNLDYSSDTEATIGPRPSYYYYNRPAIGSMSRGSGGAGGGVGAASTAALLAGAADLNKFNSLPRERPGTRLQGIRSRMGDRLVDENDGNTSAPEFDVRRGRDLRQRITASPSIFTADEYRAWLRRAPSSSAIAEQMRMTRDMFAQPRAQRFSCSAENIHDALRNTESIYSSRNHILGTGTLDRNMGLTRPISALPVRSMSSQHIGGAGSIRSPSIRRMRQLLELSAGPASPSGSILSTGGHQSPAPTPSATLPRPHRQIDINPAEFAKYKLDKPIVDIGGISGMLWIHLLAGRGLRTAPEGAAGTATQGQTRDLYCVIECDRVHKARTVVRSGDLQFDWDESFELDLVGNKQLDVLVYSWDPQHRHKLCYRGAISLSSILRQSPLHQLALKVEPRGTIYIRMRHTDPLALYKRRGLPSLRAGYPTLFGADLETVVNRESKNAPGSAPVPIVLRRCVEEVERRGLDIIGLYRLCGSATKKRLLREAFERNSRAVELTPEHVPDINVITGVLKDYLRELPEPLFTRCLFQMTVDALAVCLPDDPEGNAKLMLSILDCLPRANRATLVFLLDHLSLVVSNSERNKMSAQALATVMGPPLMLHSASAQPGADIDHAQPIAVLKYLLQIWPQPQAQHQQMAQHMGGAAGAMMGGLVTAGSMSNMAGVASGRRGESTGQRGSKVSALPADRQQLLLQQQAQLMAAGNLLRSSTSPYQLGGSVGSAIPDPSPLPLPGTPSPGSSSASTGSGSGSGKSTDTIKRGASPVSVKQVKIVDQPSSPYSIVMKKPPLQKDAPVEITTPTTQADTESTLGCKESNGTASRRGNVDFYDTHKTQAKSVVNEESSYSSKYTGSETKKIIPGNSSYTPSKANASGLSGGEDYKAMRNKSSATSSSSSSQATVLSAGSTATSAPTTSSDDSDDLVSYKSSASTNALLAQSQAMTTSQLMSKYLKREPRVQFTPIKSPESPSPPGSGDGLPKGTYQLVTPISGSSSKPGATTGAISKYTTGSVESSINANSQKLSSPSRLCNSKDSNSRTGTASSTTPATSMVSTGRRLFDSLASSSSSETETKTYIGGTTAASGAITTTIYTNDTKNSGSSSSKSGIGGGSGTGLGAVSGASSETRSFGSTLFGSSGLGNGNGSSHNHSSASPSPFTTTNGNGNHNTMHLYGTLPKNGTSTGAALFGGSANSSSYHSSASGSGAGTASSSGVSSMTGSTNSYDFYTSTSSTVSSSRPFANGGNNYHTLGTYRAQYAATNPFLDAFDEKPGSNGGNAHGEEKLGADKGHHRAAVMAFQSSGDSKNGSDEYDDIK

Target ORF information:

RefSeq Version	NM_001300700.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300700.1

ORF Insert Sequence:

ATGCAGTGGA AGAAGAAGTT TACTCGATTG AAAGCAGCTA CTGGAAATTC GCGTGTGCGA 
AGAATGCTTT GTTGTGGACG CAGAAAGGAA AATGGAAGAT CAGTTCCTGA TGTCACCGCA 
AGCCCGGGAC GGGCCCCGCC CGGACCACTG CCCGCCAACC AGATGCCTGC GATGGGCAAC 
CAGCAACACC ATGGAAACCA GCAACATCAT GGAAACCAGC AGCAACATCA TGGCAATCAG 
CACAGCAACC ATCGAGGTCA GAGCGGAAGT TTGTCAAACG CCGCCGGGGT CAAGGACCCG 
GTGATGCTAC AGGGCGATTT TCGGAAAGTC AGCGGAATCA GCTCAGAGAT CTTTCGGCAG 
ATAGAAGCCG TCGAGAATGA CCACGACCCA AACACGGCGG CGGCTTTGGA GGCGGTGGAG 
AGACGCGGTG AGATGATCGT GCGCGTCCTG GAGCCGCGTT GCATGGGCAG CAAACAGGCG 
GTGGACGCTG CCCACAAGCT GATGAACAAG GCGGACGCAC GGCACACCGT GCAGCTGGTA 
GAAATAGTCA AGCGGCCGGG CCAGACGCTG GGCTTGTACA TCCGCGAGGG AAATGGGGCC 
GATCGAACTG ATGGCGTCTT TATTTCGCGG ATTGCACTGG AGTCGGCTGT CTACAACAGC 
GGCTGCTTGC GGGTGGGCGA CGAAATCCTT GCGGTAAATC TGGTGGACGT GACACACATG 
TCCTTAGACG ATGTCGTCAT TATTATGTCA ATTCCGCGCC GCTTGGTGCT GGCTATACGT 
CAGCGGCGGG GCAATAGAGG CACAGGATCT CCTGGACCGC CAACGCTCTC TAGGCCAGAA 
CAGAAGCCCC CACCGGTTGT TGTCATCAAG CGAGATTTGC GAGACGAGGA TCTGGACGAG 
ACGGACCGGA TGCCGAGGCC GCGCTCATCA CGTGACAGAC GTACAGGAGA TGGTCGCGAA 
ATGACGGAAT CTCGTTCTAG GCTAGGTCTG GGTCTTAATA ATTACAGCCC GCAGTCCGAG 
CAGTTGGATA TGTACTACAA TACCCGCGGC GGAGGTGGAG GAGCCATGGG TGAGCCTCCA 
AACTGGGGCT ATAAACCACC ACCGCCACCG TCTTCGGTCA TTACGGAGCA ACCCACGAAA 
GCACATGCTT TTGCGCCGTC GCATGCTTAC TACCAAAATG CCGGCACTCT TGAAAGCTTG 
GCGGAGAAGG TACATGCATT CTATCCTGGA CAGCCTGGTG GTCCGCCAGT GGGTCCCTCG 
AGAAGAATGT CAACGGGAAC TGGAAACGTT GGCCTCGCTC AGCAACATGC CAGATTTCCG 
CGATCTGGCT CAGATCAGCA TTTACCTCGC GTAGAATATT CGGACTATTC CAATTCACTG 
GGGCGGCATT CCCTTCTGCG ATCAAGTTTA AAACCTGGAA CGACCGGTGG AGCTCCAATG 
CAGGTAGGAG TGGGAGGCAC TCTTGGCCGA TACGGCCGCT ATGATCAACA GAGAGCCGGT 
GTATCCAAGT ACGGCCCTCC ATCTGGTGGA GCTCAATCGC TGACCCGTCG TTCCAGACCA 
AATTTGGACT ATTCCAGCGA TACGGAAGCC ACAATTGGGC CCAGGCCAAG TTACTACTAC 
TATAACAGAC CTGCCATCGG CAGTATGTCG AGGGGATCAG GTGGAGCAGG CGGAGGAGTT 
GGCGCAGCTT CAACGGCTGC CTTGCTGGCT GGAGCTGCTG ATCTTAACAA ATTTAACTCG 
TTGCCACGAG AGCGCCCCGG GACGAGACTG CAGGGAATTC GTTCCAGAAT GGGAGATCGT 
TTGGTGGACG AAAATGACGG AAATACTTCG GCACCCGAGT TCGATGTACG GAGAGGAAGG 
GACTTACGTC AGCGAATTAC CGCCAGTCCG TCGATATTTA CGGCAGATGA ATACCGCGCC 
TGGCTCAGAA GGGCGCCTAG CAGTTCCGCA ATTGCGGAAC AAATGCGAAT GACGCGGGAC 
ATGTTTGCCC AGCCACGGGC TCAGCGATTT TCGTGTAGCG CTGAGAACAT CCATGATGCA 
CTGAGAAATA CGGAAAGCAT CTACTCCAGT AGAAACCATA TTCTTGGTAC GGGCACTCTC 
GATCGGAACA TGGGTCTTAC CCGACCAATT TCAGCACTAC CCGTGCGCTC CATGTCCTCA 
CAGCACATTG GAGGAGCGGG TTCCATTCGT TCGCCTAGCA TACGGCGCAT GCGACAGTTA 
CTGGAGCTTT CCGCTGGTCC CGCCAGTCCG AGCGGCAGTA TCTTGAGCAC CGGTGGTCAT 
CAGAGTCCGG CACCAACTCC AAGCGCGACC CTACCACGCC CGCACCGCCA AATCGACATC 
AACCCGGCGG AGTTTGCCAA GTACAAGCTG GATAAGCCCA TCGTTGATAT TGGGGGGATC 
TCCGGCATGT TATGGATTCA TTTGCTAGCA GGTCGCGGCC TAAGAACCGC TCCAGAGGGA 
GCAGCAGGAA CGGCGACACA GGGCCAAACC AGAGATCTTT ACTGCGTAAT CGAGTGCGAT 
CGAGTACATA AAGCACGGAC TGTGGTACGA TCGGGTGACC TGCAGTTCGA CTGGGACGAG 
TCGTTTGAGC TCGACTTGGT GGGCAACAAA CAGTTAGATG TACTAGTCTA CTCATGGGAC 
CCGCAGCACA GACACAAGCT GTGTTACCGA GGCGCAATCT CGTTATCGTC GATCCTCCGT 
CAGTCTCCAC TTCATCAACT GGCCTTGAAG GTTGAACCAA GAGGTACGAT ATACATTCGC 
ATGCGGCACA CGGATCCACT GGCTCTCTAC AAGAGAAGGG GACTTCCGAG CCTGAGAGCA 
GGTTACCCTA CGCTCTTCGG CGCTGATTTG GAAACGGTGG TGAATCGGGA GTCTAAGAAT 
GCTCCCGGAA GTGCACCTGT TCCCATCGTA TTAAGGCGCT GTGTGGAGGA GGTGGAACGG 
CGTGGTCTTG ATATAATCGG GTTGTACCGC CTGTGCGGCT CGGCTACCAA GAAGCGATTG 
CTACGCGAGG CCTTTGAGCG CAATAGCCGT GCAGTGGAAT TGACCCCGGA ACATGTTCCT 
GACATCAACG TCATCACCGG CGTCCTCAAG GATTACCTTA GGGAGCTGCC CGAGCCGCTG 
TTCACGCGCT GTCTTTTCCA GATGACGGTG GATGCCTTAG CTGTCTGCCT GCCAGATGAC 
CCAGAGGGCA ATGCGAAACT GATGCTTAGC ATACTCGACT GCCTACCGCG GGCGAACAGG 
GCCACCCTGG TATTCCTTCT TGATCACCTC TCGCTGGTCG TTTCAAACTC GGAGCGGAAC 
AAGATGTCAG CCCAGGCGTT GGCCACCGTG ATGGGCCCAC CGCTGATGCT GCATTCGGCG 
AGTGCGCAGC CGGGTGCTGA CATCGATCAC GCTCAGCCGA TCGCGGTGCT TAAGTATCTT 
CTGCAGATCT GGCCGCAGCC GCAGGCGCAG CATCAGCAGA TGGCGCAGCA CATGGGCGGC 
GCTGCGGGGG CAATGATGGG CGGTTTGGTC ACCGCCGGGA GCATGAGCAA TATGGCCGGT 
GTTGCTTCAG GTCGGCGCGG CGAGTCAACA GGGCAGCGTG GAAGCAAAGT CAGTGCGTTG 
CCAGCGGACA GACAGCAACT TCTACTGCAG CAGCAGGCGC AGCTCATGGC GGCGGGCAAT 
CTTCTGCGCT CGTCCACTTC GCCCTATCAA TTGGGGGGCT CAGTGGGCTC AGCAATTCCC 
GACCCATCGC CACTGCCACT TCCAGGCACA CCCTCCCCCG GCAGTAGCTC AGCATCGACG 
GGCTCAGGCT CCGGATCGGG TAAAAGCACG GACACCATCA AGCGCGGTGC TTCGCCTGTT 
TCCGTTAAGC AAGTCAAGAT CGTCGACCAG CCATCCAGCC CATACTCCAT TGTGATGAAG 
AAACCGCCGT TGCAAAAAGA TGCGCCCGTT GAAATCACCA CCCCCACCAC ACAGGCTGAT 
ACAGAGTCGA CCTTGGGATG CAAGGAAAGC AATGGAACAG CTTCTCGAAG GGGAAATGTA 
GACTTTTATG ATACGCATAA GACGCAGGCA AAGAGTGTGG TAAACGAGGA ATCCAGCTAC 
AGCTCCAAGT ACACTGGCTC GGAGACCAAG AAGATCATCC CTGGCAACAG TAGCTACACT 
CCCAGCAAGG CGAATGCTAG TGGCCTTTCT GGCGGCGAAG ACTACAAAGC AATGCGTAAC 
AAGTCGAGCG CTACATCCAG CTCCAGTTCA TCGCAGGCAA CCGTTTTGAG TGCTGGCTCT 
ACCGCCACAT CAGCCCCGAC CACTTCTTCA GACGATTCGG ATGACCTGGT CTCCTACAAG 
TCATCGGCGT CCACAAATGC CTTGCTGGCC CAATCGCAGG CCATGACCAC CAGCCAGCTG 
ATGTCCAAGT ATCTTAAGCG GGAGCCACGA GTACAGTTTA CGCCAATCAA GTCACCGGAA 
TCTCCCTCGC CACCGGGTAG CGGCGATGGT CTGCCGAAGG GTACTTACCA ACTAGTTACG 
CCTATCTCGG GATCGTCGAG CAAACCGGGT GCTACAACTG GAGCTATAAG CAAGTATACC 
ACCGGCTCAG TGGAATCATC TATTAATGCG AACAGCCAGA AGTTGTCATC GCCCTCACGA 
TTGTGCAACA GCAAGGATAG CAATAGCAGG ACTGGAACGG CCAGTAGTAC GACCCCCGCG 
ACCTCCATGG TTTCTACAGG TCGGCGTTTG TTTGACAGTC TCGCCTCTTC ATCGTCCTCG 
GAAACGGAGA CCAAGACGTA CATAGGTGGC ACCACTGCAG CCAGTGGAGC CATTACCACC 
ACCATCTACA CCAACGACAC CAAGAACTCC GGCAGCAGTA GCAGTAAGTC GGGAATAGGA 
GGAGGGTCTG GAACTGGGTT GGGAGCAGTT TCGGGAGCAA GCTCCGAGAC CCGAAGCTTC 
GGCAGTACGC TATTTGGCAG TAGTGGCTTA GGTAATGGCA ATGGTAGTAG CCACAACCAC 
TCTAGCGCCA GTCCAAGTCC CTTCACCACC ACCAACGGCA ACGGTAACCA CAATACCATG 
CATCTGTACG GCACCTTGCC CAAAAACGGA ACTTCCACGG GGGCAGCTCT GTTTGGCGGA 
TCGGCCAACA GTTCTTCGTA CCACTCTTCG GCGAGTGGAA GTGGAGCCGG AACCGCCAGC 
AGTAGTGGGG TCAGCTCTAT GACTGGCTCC ACAAACAGCT ACGACTTCTA CACGAGCACA 
AGTTCCACTG TGAGCAGTTC GCGCCCCTTT GCCAATGGAG GCAACAACTA TCACACACTG 
GGAACCTATA GGGCTCAGTA CGCCGCCACT AACCCCTTCC TGGACGCATT TGATGAAAAG 
CCGGGCAGCA ACGGGGGTAA TGCCCACGGG GAGGAGAAGC TAGGCGCGGA CAAGGGCCAC 
CACAGAGCGG CCGTTATGGC CTTTCAGTCT TCAGGAGACT CGAAAAACGG TAGCGATGAG 
TACGATGACA TCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP100F ( NM_001170314.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163785.1 Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant B, mRNA.

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CloneID	OFa07753
Clone ID Related Accession (Same CDS sequence)	NM_001170314.2
Accession Version	NM_001170314.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 100F, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170314.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTGGA AGAAGAAGTT TACTCGATTG AAAGCAGCTA CTGGAAATTC GCGTGTGCGA 
AGAATGCTTT GTTGTGGACG CAGAAAGGAA AATGGAAGAT CAGTTCCTGA TGTCACCGCA 
AGCCCGGGAC GGGCCCCGCC CGGACCACTG CCCGCCAACC AGATGCCTGC GATGGGCAAC 
CAGCAACACC ATGGAAACCA GCAACATCAT GGAAACCAGC AGCAACATCA TGGCAATCAG 
CACAGCAACC ATCGAGGTCA GAGCGGAAGT TTGTCAAACG CCGCCGGGGT CAAGGACCCG 
GTGATGCTAC AGGGCGATTT TCGGAAAGTC AGCGGAATCA GCTCAGAGAT CTTTCGGCAG 
ATAGAAGCCG TCGAGAATGA CCACGACCCA AACACGGCGG CGGCTTTGGA GGCGGTGGAG 
AGACGCGGTG AGATGATCGT GCGCGTCCTG GAGCCGCGTT GCATGGGCAG CAAACAGGCG 
GTGGACGCTG CCCACAAGCT GATGAACAAG GCGGACGCAC GGCACACCGT GCAGCTGGTA 
GAAATAGTCA AGCGGCCGGG CCAGACGCTG GGCTTGTACA TCCGCGAGGG AAATGGGGCC 
GATCGAACTG ATGGCGTCTT TATTTCGCGG ATTGCACTGG AGTCGGCTGT CTACAACAGC 
GGCTGCTTGC GGGTGGGCGA CGAAATCCTT GCGGTAAATC TGGTGGACGT GACACACATG 
TCCTTAGACG ATGTCGTCAT TATTATGTCA ATTCCGCGCC GCTTGGTGCT GGCTATACGT 
CAGCGGCGGG GCAATAGAGG CACAGGATCT CCTGGACCGC CAACGCTCTC TAGGCCAGAA 
CAGAAGCCCC CACCGGTTGT TGTCATCAAG CGAGATTTGC GAGACGAGGA TCTGGACGAG 
ACGGACCGGA TGCCGAGGCC GCGCTCATCA CGTGACAGAC GTACAGGAGA TGGTCGCGAA 
ATGACGGAAT CTCGTTCTAG GCTAGGTCTG GGTCTTAATA ATTACAGCCC GCAGTCCGAG 
CAGTTGGATA TGTACTACAA TACCCGCGGC GGAGGTGGAG GAGCCATGGG TGAGCCTCCA 
AACTGGGGCT ATAAACCACC ACCGCCACCG TCTTCGGTCA TTACGGAGCA ACCCACGAAA 
GCACATGCTT TTGCGCCGTC GCATGCTTAC TACCAAAATG CCGGCACTCT TGAAAGCTTG 
GCGGAGAAGG TACATGCATT CTATCCTGGA CAGCCTGGTG GTCCGCCAGT GGGTCCCTCG 
AGAAGAATGT CAACGGGAAC TGGAAACGTT GGCCTCGCTC AGCAACATGC CAGATTTCCG 
CGATCTGGCT CAGATCAGCA TTTACCTCGC GTAGAATATT CGGACTATTC CAATTCACTG 
GGGCGGCATT CCCTTCTGCG ATCAAGTTTA AAACCTGGAA CGACCGGTGG AGCTCCAATG 
CAGGTAGGAG TGGGAGGCAC TCTTGGCCGA TACGGCCGCT ATGATCAACA GAGAGCCGGT 
GTATCCAAGT ACGGCCCTCC ATCTGGTGGA GCTCAATCGC TGACCCGTCG TTCCAGACCA 
AATTTGGACT ATTCCAGCGA TACGGAAGCC ACAATTGGGC CCAGGCCAAG TTACTACTAC 
TATAACAGAC CTGCCATCGG CAGTATGTCG AGGGGATCAG GTGGAGCAGG CGGAGGAGTT 
GGCGCAGCTT CAACGGCTGC CTTGCTGGCT GGAGCTGCTG ATCTTAACAA ATTTAACTCG 
TTGCCACGAG AGCGCCCCGG GACGAGACTG CAGGGAATTC GTTCCAGAAT GGGAGATCGT 
TTGGTGGACG AAAATGACGG AAATACTTCG GCACCCGAGT TCGATGTACG GAGAGGAAGG 
GACTTACGTC AGCGAATTAC CGCCAGTCCG TCGATATTTA CGGCAGATGA ATACCGCGCC 
TGGCTCAGAA GGGCGCCTAG CAGTTCCGCA ATTGCGGAAC AAATGCGAAT GACGCGGGAC 
ATGTTTGCCC AGCCACGGGC TCAGCGATTT TCGTGTAGCG CTGAGAACAT CCATGATGCA 
CTGAGAAATA CGGAAAGCAT CTACTCCAGT AGAAACCATA TTCTTGGTAC GGGCACTCTC 
GATCGGAACA TGGGTCTTAC CCGACCAATT TCAGCACTAC CCGTGCGCTC CATGTCCTCA 
CAGCACATTG GAGGAGCGGG TTCCATTCGT TCGCCTAGCA TACGGCGCAT GCGACAGTTA 
CTGGAGCTTT CCGCTGGTCC CGCCAGTCCG AGCGGCAGTA TCTTGAGCAC CGGTGGTCAT 
CAGAGTCCGG CACCAACTCC AAGCGCGACC CTACCACGCC CGCACCGCCA AATCGACATC 
AACCCGGCGG AGTTTGCCAA GTACAAGCTG GATAAGCCCA TCGTTGATAT TGGGGGGATC 
TCCGGCATGT TATGGATTCA TTTGCTAGCA GGTCGCGGCC TAAGAACCGC TCCAGAGGGA 
GCAGCAGGAA CGGCGACACA GGGCCAAACC AGAGATCTTT ACTGCGTAAT CGAGTGCGAT 
CGAGTACATA AAGCACGGAC TGTGGTACGA TCGGGTGACC TGCAGTTCGA CTGGGACGAG 
TCGTTTGAGC TCGACTTGGT GGGCAACAAA CAGTTAGATG TACTAGTCTA CTCATGGGAC 
CCGCAGCACA GACACAAGCT GTGTTACCGA GGCGCAATCT CGTTATCGTC GATCCTCCGT 
CAGTCTCCAC TTCATCAACT GGCCTTGAAG GTTGAACCAA GAGGTACGAT ATACATTCGC 
ATGCGGCACA CGGATCCACT GGCTCTCTAC AAGAGAAGGG GACTTCCGAG CCTGAGAGCA 
GGTTACCCTA CGCTCTTCGG CGCTGATTTG GAAACGGTGG TGAATCGGGA GTCTAAGAAT 
GCTCCCGGAA GTGCACCTGT TCCCATCGTA TTAAGGCGCT GTGTGGAGGA GGTGGAACGG 
CGTGGTCTTG ATATAATCGG GTTGTACCGC CTGTGCGGCT CGGCTACCAA GAAGCGATTG 
CTACGCGAGG CCTTTGAGCG CAATAGCCGT GCAGTGGAAT TGACCCCGGA ACATGTTCCT 
GACATCAACG TCATCACCGG CGTCCTCAAG GATTACCTTA GGGAGCTGCC CGAGCCGCTG 
TTCACGCGCT GTCTTTTCCA GATGACGGTG GATGCCTTAG CTGTCTGCCT GCCAGATGAC 
CCAGAGGGCA ATGCGAAACT GATGCTTAGC ATACTCGACT GCCTACCGCG GGCGAACAGG 
GCCACCCTGG TATTCCTTCT TGATCACCTC TCGCTGGTCG TTTCAAACTC GGAGCGGAAC 
AAGATGTCAG CCCAGGCGTT GGCCACCGTG ATGGGCCCAC CGCTGATGCT GCATTCGGCG 
AGTGCGCAGC CGGGTGCTGA CATCGATCAC GCTCAGCCGA TCGCGGTGCT TAAGTATCTT 
CTGCAGATCT GGCCGCAGCC GCAGGCGCAG CATCAGCAGA TGGCGCAGCA CATGGGCGGC 
GCTGCGGGGG CAATGATGGG CGGTTTGGTC ACCGCCGGGA GCATGAGCAA TATGGCCGGT 
GTTGCTTCAG GTAACGTACT CCCAGCGTCC GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163785.1
CDS	680..4255
Translation	MQWKKKFTRLKAATGNSRVRRMLCCGRRKENGRSVPDVTASPGRAPPGPLPANQMPAMGNQQHHGNQQHHGNQQQHHGNQHSNHRGQSGSLSNAAGVKDPVMLQGDFRKVSGISSEIFRQIEAVENDHDPNTAAALEAVERRGEMIVRVLEPRCMGSKQAVDAAHKLMNKADARHTVQLVEIVKRPGQTLGLYIREGNGADRTDGVFISRIALESAVYNSGCLRVGDEILAVNLVDVTHMSLDDVVIIMSIPRRLVLAIRQRRGNRGTGSPGPPTLSRPEQKPPPVVVIKRDLRDEDLDETDRMPRPRSSRDRRTGDGREMTESRSRLGLGLNNYSPQSEQLDMYYNTRGGGGGAMGEPPNWGYKPPPPPSSVITEQPTKAHAFAPSHAYYQNAGTLESLAEKVHAFYPGQPGGPPVGPSRRMSTGTGNVGLAQQHARFPRSGSDQHLPRVEYSDYSNSLGRHSLLRSSLKPGTTGGAPMQVGVGGTLGRYGRYDQQRAGVSKYGPPSGGAQSLTRRSRPNLDYSSDTEATIGPRPSYYYYNRPAIGSMSRGSGGAGGGVGAASTAALLAGAADLNKFNSLPRERPGTRLQGIRSRMGDRLVDENDGNTSAPEFDVRRGRDLRQRITASPSIFTADEYRAWLRRAPSSSAIAEQMRMTRDMFAQPRAQRFSCSAENIHDALRNTESIYSSRNHILGTGTLDRNMGLTRPISALPVRSMSSQHIGGAGSIRSPSIRRMRQLLELSAGPASPSGSILSTGGHQSPAPTPSATLPRPHRQIDINPAEFAKYKLDKPIVDIGGISGMLWIHLLAGRGLRTAPEGAAGTATQGQTRDLYCVIECDRVHKARTVVRSGDLQFDWDESFELDLVGNKQLDVLVYSWDPQHRHKLCYRGAISLSSILRQSPLHQLALKVEPRGTIYIRMRHTDPLALYKRRGLPSLRAGYPTLFGADLETVVNRESKNAPGSAPVPIVLRRCVEEVERRGLDIIGLYRLCGSATKKRLLREAFERNSRAVELTPEHVPDINVITGVLKDYLRELPEPLFTRCLFQMTVDALAVCLPDDPEGNAKLMLSILDCLPRANRATLVFLLDHLSLVVSNSERNKMSAQALATVMGPPLMLHSASAQPGADIDHAQPIAVLKYLLQIWPQPQAQHQQMAQHMGGAAGAMMGGLVTAGSMSNMAGVASGNVLPASD

Target ORF information:

RefSeq Version	NM_001170314.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170314.2

ORF Insert Sequence:

ATGCAGTGGA AGAAGAAGTT TACTCGATTG AAAGCAGCTA CTGGAAATTC GCGTGTGCGA 
AGAATGCTTT GTTGTGGACG CAGAAAGGAA AATGGAAGAT CAGTTCCTGA TGTCACCGCA 
AGCCCGGGAC GGGCCCCGCC CGGACCACTG CCCGCCAACC AGATGCCTGC GATGGGCAAC 
CAGCAACACC ATGGAAACCA GCAACATCAT GGAAACCAGC AGCAACATCA TGGCAATCAG 
CACAGCAACC ATCGAGGTCA GAGCGGAAGT TTGTCAAACG CCGCCGGGGT CAAGGACCCG 
GTGATGCTAC AGGGCGATTT TCGGAAAGTC AGCGGAATCA GCTCAGAGAT CTTTCGGCAG 
ATAGAAGCCG TCGAGAATGA CCACGACCCA AACACGGCGG CGGCTTTGGA GGCGGTGGAG 
AGACGCGGTG AGATGATCGT GCGCGTCCTG GAGCCGCGTT GCATGGGCAG CAAACAGGCG 
GTGGACGCTG CCCACAAGCT GATGAACAAG GCGGACGCAC GGCACACCGT GCAGCTGGTA 
GAAATAGTCA AGCGGCCGGG CCAGACGCTG GGCTTGTACA TCCGCGAGGG AAATGGGGCC 
GATCGAACTG ATGGCGTCTT TATTTCGCGG ATTGCACTGG AGTCGGCTGT CTACAACAGC 
GGCTGCTTGC GGGTGGGCGA CGAAATCCTT GCGGTAAATC TGGTGGACGT GACACACATG 
TCCTTAGACG ATGTCGTCAT TATTATGTCA ATTCCGCGCC GCTTGGTGCT GGCTATACGT 
CAGCGGCGGG GCAATAGAGG CACAGGATCT CCTGGACCGC CAACGCTCTC TAGGCCAGAA 
CAGAAGCCCC CACCGGTTGT TGTCATCAAG CGAGATTTGC GAGACGAGGA TCTGGACGAG 
ACGGACCGGA TGCCGAGGCC GCGCTCATCA CGTGACAGAC GTACAGGAGA TGGTCGCGAA 
ATGACGGAAT CTCGTTCTAG GCTAGGTCTG GGTCTTAATA ATTACAGCCC GCAGTCCGAG 
CAGTTGGATA TGTACTACAA TACCCGCGGC GGAGGTGGAG GAGCCATGGG TGAGCCTCCA 
AACTGGGGCT ATAAACCACC ACCGCCACCG TCTTCGGTCA TTACGGAGCA ACCCACGAAA 
GCACATGCTT TTGCGCCGTC GCATGCTTAC TACCAAAATG CCGGCACTCT TGAAAGCTTG 
GCGGAGAAGG TACATGCATT CTATCCTGGA CAGCCTGGTG GTCCGCCAGT GGGTCCCTCG 
AGAAGAATGT CAACGGGAAC TGGAAACGTT GGCCTCGCTC AGCAACATGC CAGATTTCCG 
CGATCTGGCT CAGATCAGCA TTTACCTCGC GTAGAATATT CGGACTATTC CAATTCACTG 
GGGCGGCATT CCCTTCTGCG ATCAAGTTTA AAACCTGGAA CGACCGGTGG AGCTCCAATG 
CAGGTAGGAG TGGGAGGCAC TCTTGGCCGA TACGGCCGCT ATGATCAACA GAGAGCCGGT 
GTATCCAAGT ACGGCCCTCC ATCTGGTGGA GCTCAATCGC TGACCCGTCG TTCCAGACCA 
AATTTGGACT ATTCCAGCGA TACGGAAGCC ACAATTGGGC CCAGGCCAAG TTACTACTAC 
TATAACAGAC CTGCCATCGG CAGTATGTCG AGGGGATCAG GTGGAGCAGG CGGAGGAGTT 
GGCGCAGCTT CAACGGCTGC CTTGCTGGCT GGAGCTGCTG ATCTTAACAA ATTTAACTCG 
TTGCCACGAG AGCGCCCCGG GACGAGACTG CAGGGAATTC GTTCCAGAAT GGGAGATCGT 
TTGGTGGACG AAAATGACGG AAATACTTCG GCACCCGAGT TCGATGTACG GAGAGGAAGG 
GACTTACGTC AGCGAATTAC CGCCAGTCCG TCGATATTTA CGGCAGATGA ATACCGCGCC 
TGGCTCAGAA GGGCGCCTAG CAGTTCCGCA ATTGCGGAAC AAATGCGAAT GACGCGGGAC 
ATGTTTGCCC AGCCACGGGC TCAGCGATTT TCGTGTAGCG CTGAGAACAT CCATGATGCA 
CTGAGAAATA CGGAAAGCAT CTACTCCAGT AGAAACCATA TTCTTGGTAC GGGCACTCTC 
GATCGGAACA TGGGTCTTAC CCGACCAATT TCAGCACTAC CCGTGCGCTC CATGTCCTCA 
CAGCACATTG GAGGAGCGGG TTCCATTCGT TCGCCTAGCA TACGGCGCAT GCGACAGTTA 
CTGGAGCTTT CCGCTGGTCC CGCCAGTCCG AGCGGCAGTA TCTTGAGCAC CGGTGGTCAT 
CAGAGTCCGG CACCAACTCC AAGCGCGACC CTACCACGCC CGCACCGCCA AATCGACATC 
AACCCGGCGG AGTTTGCCAA GTACAAGCTG GATAAGCCCA TCGTTGATAT TGGGGGGATC 
TCCGGCATGT TATGGATTCA TTTGCTAGCA GGTCGCGGCC TAAGAACCGC TCCAGAGGGA 
GCAGCAGGAA CGGCGACACA GGGCCAAACC AGAGATCTTT ACTGCGTAAT CGAGTGCGAT 
CGAGTACATA AAGCACGGAC TGTGGTACGA TCGGGTGACC TGCAGTTCGA CTGGGACGAG 
TCGTTTGAGC TCGACTTGGT GGGCAACAAA CAGTTAGATG TACTAGTCTA CTCATGGGAC 
CCGCAGCACA GACACAAGCT GTGTTACCGA GGCGCAATCT CGTTATCGTC GATCCTCCGT 
CAGTCTCCAC TTCATCAACT GGCCTTGAAG GTTGAACCAA GAGGTACGAT ATACATTCGC 
ATGCGGCACA CGGATCCACT GGCTCTCTAC AAGAGAAGGG GACTTCCGAG CCTGAGAGCA 
GGTTACCCTA CGCTCTTCGG CGCTGATTTG GAAACGGTGG TGAATCGGGA GTCTAAGAAT 
GCTCCCGGAA GTGCACCTGT TCCCATCGTA TTAAGGCGCT GTGTGGAGGA GGTGGAACGG 
CGTGGTCTTG ATATAATCGG GTTGTACCGC CTGTGCGGCT CGGCTACCAA GAAGCGATTG 
CTACGCGAGG CCTTTGAGCG CAATAGCCGT GCAGTGGAAT TGACCCCGGA ACATGTTCCT 
GACATCAACG TCATCACCGG CGTCCTCAAG GATTACCTTA GGGAGCTGCC CGAGCCGCTG 
TTCACGCGCT GTCTTTTCCA GATGACGGTG GATGCCTTAG CTGTCTGCCT GCCAGATGAC 
CCAGAGGGCA ATGCGAAACT GATGCTTAGC ATACTCGACT GCCTACCGCG GGCGAACAGG 
GCCACCCTGG TATTCCTTCT TGATCACCTC TCGCTGGTCG TTTCAAACTC GGAGCGGAAC 
AAGATGTCAG CCCAGGCGTT GGCCACCGTG ATGGGCCCAC CGCTGATGCT GCATTCGGCG 
AGTGCGCAGC CGGGTGCTGA CATCGATCAC GCTCAGCCGA TCGCGGTGCT TAAGTATCTT 
CTGCAGATCT GGCCGCAGCC GCAGGCGCAG CATCAGCAGA TGGCGCAGCA CATGGGCGGC 
GCTGCGGGGG CAATGATGGG CGGTTTGGTC ACCGCCGGGA GCATGAGCAA TATGGCCGGT 
GTTGCTTCAG GTAACGTACT CCCAGCGTCC GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP100F ( NM_001260466.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247395.1 Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant E, mRNA.

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CloneID	OFa07755
Clone ID Related Accession (Same CDS sequence)	NM_001260466.1
Accession Version	NM_001260466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 100F, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTGATA GTGCGACCAC GGGATGTTTC CTGACCAGAA GCAGCCATCG CAAGGAAAAT 
GGAAGATCAG TTCCTGATGT CACCGCAAGC CCGGGACGGG CCCCGCCCGG ACCACTGCCC 
GCCAACCAGA TGCCTGCGAT GGGCAACCAG CAACACCATG GAAACCAGCA ACATCATGGA 
AACCAGCAGC AACATCATGG CAATCAGCAC AGCAACCATC GAGGTCAGAG CGGAAGTTTG 
TCAAACGCCG CCGGGGTCAA GGACCCGGTG ATGCTACAGG GCGATTTTCG GAAAGTCAGC 
GGAATCAGCT CAGAGATCTT TCGGCAGATA GAAGCCGTCG AGAATGACCA CGACCCAAAC 
ACGGCGGCGG CTTTGGAGGC GGTGGAGAGA CGCGGTGAGA TGATCGTGCG CGTCCTGGAG 
CCGCGTTGCA TGGGCAGCAA ACAGGCGGTG GACGCTGCCC ACAAGCTGAT GAACAAGGCG 
GACGCACGGC ACACCGTGCA GCTGGTAGAA ATAGTCAAGC GGCCGGGCCA GACGCTGGGC 
TTGTACATCC GCGAGGGAAA TGGGGCCGAT CGAACTGATG GCGTCTTTAT TTCGCGGATT 
GCACTGGAGT CGGCTGTCTA CAACAGCGGC TGCTTGCGGG TGGGCGACGA AATCCTTGCG 
GTAAATCTGG TGGACGTGAC ACACATGTCC TTAGACGATG TCGTCATTAT TATGTCAATT 
CCGCGCCGCT TGGTGCTGGC TATACGTCAG CGGCGGGGCA ATAGAGGCAC AGGATCTCCT 
GGACCGCCAA CGCTCTCTAG GCCAGAACAG AAGCCCCCAC CGGTTGTTGT CATCAAGCGA 
GATTTGCGAG ACGAGGATCT GGACGAGACG GACCGGATGC CGAGGCCGCG CTCATCACGT 
GACAGACGTA CAGGAGATGG TCGCGAAATG ACGGAATCTC GTTCTAGGCT AGGTCTGGGT 
CTTAATAATT ACAGCCCGCA GTCCGAGCAG TTGGATATGT ACTACAATAC CCGCGGCGGA 
GGTGGAGGAG CCATGGGTGA GCCTCCAAAC TGGGGCTATA AACCACCACC GCCACCGTCT 
TCGGTCATTA CGGAGCAACC CACGAAAGCA CATGCTTTTG CGCCGTCGCA TGCTTACTAC 
CAAAATGCCG GCACTCTTGA AAGCTTGGCG GAGAAGGTAC ATGCATTCTA TCCTGGACAG 
CCTGGTGGTC CGCCAGTGGG TCCCTCGAGA AGAATGTCAA CGGGAACTGG AAACGTTGGC 
CTCGCTCAGC AACATGCCAG ATTTCCGCGA TCTGGCTCAG ATCAGCATTT ACCTCGCGTA 
GAATATTCGG ACTATTCCAA TTCACTGGGG CGGCATTCCC TTCTGCGATC AAGTTTAAAA 
CCTGGAACGA CCGGTGGAGC TCCAATGCAG GTAGGAGTGG GAGGCACTCT TGGCCGATAC 
GGCCGCTATG ATCAACAGAG AGCCGGTGTA TCCAAGTACG GCCCTCCATC TGGTGGAGCT 
CAATCGCTGA CCCGTCGTTC CAGACCAAAT TTGGACTATT CCAGCGATAC GGAAGCCACA 
ATTGGGCCCA GGCCAAGTTA CTACTACTAT AACAGACCTG CCATCGGCAG TATGTCGAGG 
GGATCAGGTG GAGCAGGCGG AGGAGTTGGC GCAGCTTCAA CGGCTGCCTT GCTGGCTGGA 
GCTGCTGATC TTAACAAATT TAACTCGTTG CCACGAGAGC GCCCCGGGAC GAGACTGCAG 
GGAATTCGTT CCAGAATGGG AGATCGTTTG GTGGACGAAA ATGACGGAAA TACTTCGGCA 
CCCGAGTTCG ATGTACGGAG AGGAAGGGAC TTACGTCAGC GAATTACCGC CAGTCCGTCG 
ATATTTACGG CAGATGAATA CCGCGCCTGG CTCAGAAGGG CGCCTAGCAG TTCCGCAATT 
GCGGAACAAA TGCGAATGAC GCGGGACATG TTTGCCCAGC CACGGGCTCA GCGATTTTCG 
TGTAGCGCTG AGAACATCCA TGATGCACTG AGAAATACGG AAAGCATCTA CTCCAGTAGA 
AACCATATTC TTGGTACGGG CACTCTCGAT CGGAACATGG GTCTTACCCG ACCAATTTCA 
GCACTACCCG TGCGCTCCAT GTCCTCACAG CACATTGGAG GAGCGGGTTC CATTCGTTCG 
CCTAGCATAC GGCGCATGCG ACAGTTACTG GAGCTTTCCG CTGGTCCCGC CAGTCCGAGC 
GGCAGTATCT TGAGCACCGG TGGTCATCAG AGTCCGGCAC CAACTCCAAG CGCGACCCTA 
CCACGCCCGC ACCGCCAAAT CGACATCAAC CCGGCGGAGT TTGCCAAGTA CAAGCTGGAT 
AAGCCCATCG TTGATATTGG GGGGATCTCC GGCATGTTAT GGATTCATTT GCTAGCAGGT 
CGCGGCCTAA GAACCGCTCC AGAGGGAGCA GCAGGAACGG CGACACAGGG CCAAACCAGA 
GATCTTTACT GCGTAATCGA GTGCGATCGA GTACATAAAG CACGGACTGT GGTACGATCG 
GGTGACCTGC AGTTCGACTG GGACGAGTCG TTTGAGCTCG ACTTGGTGGG CAACAAACAG 
TTAGATGTAC TAGTCTACTC ATGGGACCCG CAGCACAGAC ACAAGCTGTG TTACCGAGGC 
GCAATCTCGT TATCGTCGAT CCTCCGTCAG TCTCCACTTC ATCAACTGGC CTTGAAGGTT 
GAACCAAGAG GTACGATATA CATTCGCATG CGGCACACGG ATCCACTGGC TCTCTACAAG 
AGAAGGGGAC TTCCGAGCCT GAGAGCAGGT TACCCTACGC TCTTCGGCGC TGATTTGGAA 
ACGGTGGTGA ATCGGGAGTC TAAGAATGCT CCCGGAAGTG CACCTGTTCC CATCGTATTA 
AGGCGCTGTG TGGAGGAGGT GGAACGGCGT GGTCTTGATA TAATCGGGTT GTACCGCCTG 
TGCGGCTCGG CTACCAAGAA GCGATTGCTA CGCGAGGCCT TTGAGCGCAA TAGCCGTGCA 
GTGGAATTGA CCCCGGAACA TGTTCCTGAC ATCAACGTCA TCACCGGCGT CCTCAAGGAT 
TACCTTAGGG AGCTGCCCGA GCCGCTGTTC ACGCGCTGTC TTTTCCAGAT GACGGTGGAT 
GCCTTAGCTG TCTGCCTGCC AGATGACCCA GAGGGCAATG CGAAACTGAT GCTTAGCATA 
CTCGACTGCC TACCGCGGGC GAACAGGGCC ACCCTGGTAT TCCTTCTTGA TCACCTCTCG 
CTGGTCGTTT CAAACTCGGA GCGGAACAAG ATGTCAGCCC AGGCGTTGGC CACCGTGATG 
GGCCCACCGC TGATGCTGCA TTCGGCGAGT GCGCAGCCGG GTGCTGACAT CGATCACGCT 
CAGCCGATCG CGGTGCTTAA GTATCTTCTG CAGATCTGGC CGCAGCCGCA GGCGCAGCAT 
CAGCAGATGG CGCAGCACAT GGGCGGCGCT GCGGGGGCAA TGATGGGCGG TTTGGTCACC 
GCCGGGAGCA TGAGCAATAT GGCCGGTGTT GCTTCAGCCC TATCAATTGG GGGGCTCAGT 
GGGCTCAGCA ATTCCCGACC CATCGCCACT GCCACTTCCA GGCACACCCT CCCCCGGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247395.1
CDS	790..4392
Translation	MCDSATTGCFLTRSSHRKENGRSVPDVTASPGRAPPGPLPANQMPAMGNQQHHGNQQHHGNQQQHHGNQHSNHRGQSGSLSNAAGVKDPVMLQGDFRKVSGISSEIFRQIEAVENDHDPNTAAALEAVERRGEMIVRVLEPRCMGSKQAVDAAHKLMNKADARHTVQLVEIVKRPGQTLGLYIREGNGADRTDGVFISRIALESAVYNSGCLRVGDEILAVNLVDVTHMSLDDVVIIMSIPRRLVLAIRQRRGNRGTGSPGPPTLSRPEQKPPPVVVIKRDLRDEDLDETDRMPRPRSSRDRRTGDGREMTESRSRLGLGLNNYSPQSEQLDMYYNTRGGGGGAMGEPPNWGYKPPPPPSSVITEQPTKAHAFAPSHAYYQNAGTLESLAEKVHAFYPGQPGGPPVGPSRRMSTGTGNVGLAQQHARFPRSGSDQHLPRVEYSDYSNSLGRHSLLRSSLKPGTTGGAPMQVGVGGTLGRYGRYDQQRAGVSKYGPPSGGAQSLTRRSRPNLDYSSDTEATIGPRPSYYYYNRPAIGSMSRGSGGAGGGVGAASTAALLAGAADLNKFNSLPRERPGTRLQGIRSRMGDRLVDENDGNTSAPEFDVRRGRDLRQRITASPSIFTADEYRAWLRRAPSSSAIAEQMRMTRDMFAQPRAQRFSCSAENIHDALRNTESIYSSRNHILGTGTLDRNMGLTRPISALPVRSMSSQHIGGAGSIRSPSIRRMRQLLELSAGPASPSGSILSTGGHQSPAPTPSATLPRPHRQIDINPAEFAKYKLDKPIVDIGGISGMLWIHLLAGRGLRTAPEGAAGTATQGQTRDLYCVIECDRVHKARTVVRSGDLQFDWDESFELDLVGNKQLDVLVYSWDPQHRHKLCYRGAISLSSILRQSPLHQLALKVEPRGTIYIRMRHTDPLALYKRRGLPSLRAGYPTLFGADLETVVNRESKNAPGSAPVPIVLRRCVEEVERRGLDIIGLYRLCGSATKKRLLREAFERNSRAVELTPEHVPDINVITGVLKDYLRELPEPLFTRCLFQMTVDALAVCLPDDPEGNAKLMLSILDCLPRANRATLVFLLDHLSLVVSNSERNKMSAQALATVMGPPLMLHSASAQPGADIDHAQPIAVLKYLLQIWPQPQAQHQQMAQHMGGAAGAMMGGLVTAGSMSNMAGVASALSIGGLSGLSNSRPIATATSRHTLPRQ

Target ORF information:

RefSeq Version	NM_001260466.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260466.1

ORF Insert Sequence:

ATGTGTGATA GTGCGACCAC GGGATGTTTC CTGACCAGAA GCAGCCATCG CAAGGAAAAT 
GGAAGATCAG TTCCTGATGT CACCGCAAGC CCGGGACGGG CCCCGCCCGG ACCACTGCCC 
GCCAACCAGA TGCCTGCGAT GGGCAACCAG CAACACCATG GAAACCAGCA ACATCATGGA 
AACCAGCAGC AACATCATGG CAATCAGCAC AGCAACCATC GAGGTCAGAG CGGAAGTTTG 
TCAAACGCCG CCGGGGTCAA GGACCCGGTG ATGCTACAGG GCGATTTTCG GAAAGTCAGC 
GGAATCAGCT CAGAGATCTT TCGGCAGATA GAAGCCGTCG AGAATGACCA CGACCCAAAC 
ACGGCGGCGG CTTTGGAGGC GGTGGAGAGA CGCGGTGAGA TGATCGTGCG CGTCCTGGAG 
CCGCGTTGCA TGGGCAGCAA ACAGGCGGTG GACGCTGCCC ACAAGCTGAT GAACAAGGCG 
GACGCACGGC ACACCGTGCA GCTGGTAGAA ATAGTCAAGC GGCCGGGCCA GACGCTGGGC 
TTGTACATCC GCGAGGGAAA TGGGGCCGAT CGAACTGATG GCGTCTTTAT TTCGCGGATT 
GCACTGGAGT CGGCTGTCTA CAACAGCGGC TGCTTGCGGG TGGGCGACGA AATCCTTGCG 
GTAAATCTGG TGGACGTGAC ACACATGTCC TTAGACGATG TCGTCATTAT TATGTCAATT 
CCGCGCCGCT TGGTGCTGGC TATACGTCAG CGGCGGGGCA ATAGAGGCAC AGGATCTCCT 
GGACCGCCAA CGCTCTCTAG GCCAGAACAG AAGCCCCCAC CGGTTGTTGT CATCAAGCGA 
GATTTGCGAG ACGAGGATCT GGACGAGACG GACCGGATGC CGAGGCCGCG CTCATCACGT 
GACAGACGTA CAGGAGATGG TCGCGAAATG ACGGAATCTC GTTCTAGGCT AGGTCTGGGT 
CTTAATAATT ACAGCCCGCA GTCCGAGCAG TTGGATATGT ACTACAATAC CCGCGGCGGA 
GGTGGAGGAG CCATGGGTGA GCCTCCAAAC TGGGGCTATA AACCACCACC GCCACCGTCT 
TCGGTCATTA CGGAGCAACC CACGAAAGCA CATGCTTTTG CGCCGTCGCA TGCTTACTAC 
CAAAATGCCG GCACTCTTGA AAGCTTGGCG GAGAAGGTAC ATGCATTCTA TCCTGGACAG 
CCTGGTGGTC CGCCAGTGGG TCCCTCGAGA AGAATGTCAA CGGGAACTGG AAACGTTGGC 
CTCGCTCAGC AACATGCCAG ATTTCCGCGA TCTGGCTCAG ATCAGCATTT ACCTCGCGTA 
GAATATTCGG ACTATTCCAA TTCACTGGGG CGGCATTCCC TTCTGCGATC AAGTTTAAAA 
CCTGGAACGA CCGGTGGAGC TCCAATGCAG GTAGGAGTGG GAGGCACTCT TGGCCGATAC 
GGCCGCTATG ATCAACAGAG AGCCGGTGTA TCCAAGTACG GCCCTCCATC TGGTGGAGCT 
CAATCGCTGA CCCGTCGTTC CAGACCAAAT TTGGACTATT CCAGCGATAC GGAAGCCACA 
ATTGGGCCCA GGCCAAGTTA CTACTACTAT AACAGACCTG CCATCGGCAG TATGTCGAGG 
GGATCAGGTG GAGCAGGCGG AGGAGTTGGC GCAGCTTCAA CGGCTGCCTT GCTGGCTGGA 
GCTGCTGATC TTAACAAATT TAACTCGTTG CCACGAGAGC GCCCCGGGAC GAGACTGCAG 
GGAATTCGTT CCAGAATGGG AGATCGTTTG GTGGACGAAA ATGACGGAAA TACTTCGGCA 
CCCGAGTTCG ATGTACGGAG AGGAAGGGAC TTACGTCAGC GAATTACCGC CAGTCCGTCG 
ATATTTACGG CAGATGAATA CCGCGCCTGG CTCAGAAGGG CGCCTAGCAG TTCCGCAATT 
GCGGAACAAA TGCGAATGAC GCGGGACATG TTTGCCCAGC CACGGGCTCA GCGATTTTCG 
TGTAGCGCTG AGAACATCCA TGATGCACTG AGAAATACGG AAAGCATCTA CTCCAGTAGA 
AACCATATTC TTGGTACGGG CACTCTCGAT CGGAACATGG GTCTTACCCG ACCAATTTCA 
GCACTACCCG TGCGCTCCAT GTCCTCACAG CACATTGGAG GAGCGGGTTC CATTCGTTCG 
CCTAGCATAC GGCGCATGCG ACAGTTACTG GAGCTTTCCG CTGGTCCCGC CAGTCCGAGC 
GGCAGTATCT TGAGCACCGG TGGTCATCAG AGTCCGGCAC CAACTCCAAG CGCGACCCTA 
CCACGCCCGC ACCGCCAAAT CGACATCAAC CCGGCGGAGT TTGCCAAGTA CAAGCTGGAT 
AAGCCCATCG TTGATATTGG GGGGATCTCC GGCATGTTAT GGATTCATTT GCTAGCAGGT 
CGCGGCCTAA GAACCGCTCC AGAGGGAGCA GCAGGAACGG CGACACAGGG CCAAACCAGA 
GATCTTTACT GCGTAATCGA GTGCGATCGA GTACATAAAG CACGGACTGT GGTACGATCG 
GGTGACCTGC AGTTCGACTG GGACGAGTCG TTTGAGCTCG ACTTGGTGGG CAACAAACAG 
TTAGATGTAC TAGTCTACTC ATGGGACCCG CAGCACAGAC ACAAGCTGTG TTACCGAGGC 
GCAATCTCGT TATCGTCGAT CCTCCGTCAG TCTCCACTTC ATCAACTGGC CTTGAAGGTT 
GAACCAAGAG GTACGATATA CATTCGCATG CGGCACACGG ATCCACTGGC TCTCTACAAG 
AGAAGGGGAC TTCCGAGCCT GAGAGCAGGT TACCCTACGC TCTTCGGCGC TGATTTGGAA 
ACGGTGGTGA ATCGGGAGTC TAAGAATGCT CCCGGAAGTG CACCTGTTCC CATCGTATTA 
AGGCGCTGTG TGGAGGAGGT GGAACGGCGT GGTCTTGATA TAATCGGGTT GTACCGCCTG 
TGCGGCTCGG CTACCAAGAA GCGATTGCTA CGCGAGGCCT TTGAGCGCAA TAGCCGTGCA 
GTGGAATTGA CCCCGGAACA TGTTCCTGAC ATCAACGTCA TCACCGGCGT CCTCAAGGAT 
TACCTTAGGG AGCTGCCCGA GCCGCTGTTC ACGCGCTGTC TTTTCCAGAT GACGGTGGAT 
GCCTTAGCTG TCTGCCTGCC AGATGACCCA GAGGGCAATG CGAAACTGAT GCTTAGCATA 
CTCGACTGCC TACCGCGGGC GAACAGGGCC ACCCTGGTAT TCCTTCTTGA TCACCTCTCG 
CTGGTCGTTT CAAACTCGGA GCGGAACAAG ATGTCAGCCC AGGCGTTGGC CACCGTGATG 
GGCCCACCGC TGATGCTGCA TTCGGCGAGT GCGCAGCCGG GTGCTGACAT CGATCACGCT 
CAGCCGATCG CGGTGCTTAA GTATCTTCTG CAGATCTGGC CGCAGCCGCA GGCGCAGCAT 
CAGCAGATGG CGCAGCACAT GGGCGGCGCT GCGGGGGCAA TGATGGGCGG TTTGGTCACC 
GCCGGGAGCA TGAGCAATAT GGCCGGTGTT GCTTCAGCCC TATCAATTGG GGGGCTCAGT 
GGGCTCAGCA ATTCCCGACC CATCGCCACT GCCACTTCCA GGCACACCCT CCCCCGGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP100F ( NM_143645.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651902.2 Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant C, mRNA.

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CloneID	OFa06813
Clone ID Related Accession (Same CDS sequence)	NM_143645.2
Accession Version	NM_143645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 100F, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_143645.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTGATA GTGCGACCAC GGGATGTTTC CTGACCAGAA GCAGCCATCG CAAGGAAAAT 
GGAAGATCAG TTCCTGATGT CACCGCAAGC CCGGGACGGG CCCCGCCCGG ACCACTGCCC 
GCCAACCAGA TGCCTGCGAT GGGCAACCAG CAACACCATG GAAACCAGCA ACATCATGGA 
AACCAGCAGC AACATCATGG CAATCAGCAC AGCAACCATC GAGGTCAGAG CGGAAGTTTG 
TCAAACGCCG CCGGGGTCAA GGACCCGGTG ATGCTACAGG GCGATTTTCG GAAAGTCAGC 
GGAATCAGCT CAGAGATCTT TCGGCAGATA GAAGCCGTCG AGAATGACCA CGACCCAAAC 
ACGGCGGCGG CTTTGGAGGC GGTGGAGAGA CGCGGTGAGA TGATCGTGCG CGTCCTGGAG 
CCGCGTTGCA TGGGCAGCAA ACAGGCGGTG GACGCTGCCC ACAAGCTGAT GAACAAGGCG 
GACGCACGGC ACACCGTGCA GCTGGTAGAA ATAGTCAAGC GGCCGGGCCA GACGCTGGGC 
TTGTACATCC GCGAGGGAAA TGGGGCCGAT CGAACTGATG GCGTCTTTAT TTCGCGGATT 
GCACTGGAGT CGGCTGTCTA CAACAGCGGC TGCTTGCGGG TGGGCGACGA AATCCTTGCG 
GTAAATCTGG TGGACGTGAC ACACATGTCC TTAGACGATG TCGTCATTAT TATGTCAATT 
CCGCGCCGCT TGGTGCTGGC TATACGTCAG CGGCGGGGCA ATAGAGGCAC AGGATCTCCT 
GGACCGCCAA CGCTCTCTAG GCCAGAACAG AAGCCCCCAC CGGTTGTTGT CATCAAGCGA 
GATTTGCGAG ACGAGGATCT GGACGAGACG GACCGGATGC CGAGGCCGCG CTCATCACGT 
GACAGACGTA CAGGAGATGG TCGCGAAATG ACGGAATCTC GTTCTAGGCT AGGTCTGGGT 
CTTAATAATT ACAGCCCGCA GTCCGAGCAG TTGGATATGT ACTACAATAC CCGCGGCGGA 
GGTGGAGGAG CCATGGGTGA GCCTCCAAAC TGGGGCTATA AACCACCACC GCCACCGTCT 
TCGGTCATTA CGGAGCAACC CACGAAAGCA CATGCTTTTG CGCCGTCGCA TGCTTACTAC 
CAAAATGCCG GCACTCTTGA AAGCTTGGCG GAGAAGGTAC ATGCATTCTA TCCTGGACAG 
CCTGGTGGTC CGCCAGTGGG TCCCTCGAGA AGAATGTCAA CGGGAACTGG AAACGTTGGC 
CTCGCTCAGC AACATGCCAG ATTTCCGCGA TCTGGCTCAG ATCAGCATTT ACCTCGCGTA 
GAATATTCGG ACTATTCCAA TTCACTGGGG CGGCATTCCC TTCTGCGATC AAGTTTAAAA 
CCTGGAACGA CCGGTGGAGC TCCAATGCAG GTAGGAGTGG GAGGCACTCT TGGCCGATAC 
GGCCGCTATG ATCAACAGAG AGCCGGTGTA TCCAAGTACG GCCCTCCATC TGGTGGAGCT 
CAATCGCTGA CCCGTCGTTC CAGACCAAAT TTGGACTATT CCAGCGATAC GGAAGCCACA 
ATTGGGCCCA GGCCAAGTTA CTACTACTAT AACAGACCTG CCATCGGCAG TATGTCGAGG 
GGATCAGGTG GAGCAGGCGG AGGAGTTGGC GCAGCTTCAA CGGCTGCCTT GCTGGCTGGA 
GCTGCTGATC TTAACAAATT TAACTCGTTG CCACGAGAGC GCCCCGGGAC GAGACTGCAG 
GGAATTCGTT CCAGAATGGG AGATCGTTTG GTGGACGAAA ATGACGGAAA TACTTCGGCA 
CCCGAGTTCG ATGTACGGAG AGGAAGGGAC TTACGTCAGC GAATTACCGC CAGTCCGTCG 
ATATTTACGG CAGATGAATA CCGCGCCTGG CTCAGAAGGG CGCCTAGCAG TTCCGCAATT 
GCGGAACAAA TGCGAATGAC GCGGGACATG TTTGCCCAGC CACGGGCTCA GCGATTTTCG 
TGTAGCGCTG AGAACATCCA TGATGCACTG AGAAATACGG AAAGCATCTA CTCCAGTAGA 
AACCATATTC TTGGTACGGG CACTCTCGAT CGGAACATGG GTCTTACCCG ACCAATTTCA 
GCACTACCCG TGCGCTCCAT GTCCTCACAG CACATTGGAG GAGCGGGTTC CATTCGTTCG 
CCTAGCATAC GGCGCATGCG ACAGTTACTG GAGCTTTCCG CTGGTCCCGC CAGTCCGAGC 
GGCAGTATCT TGAGCACCGG TGGTCATCAG AGTCCGGCAC CAACTCCAAG CGCGACCCTA 
CCACGCCCGC ACCGCCAAAT CGACATCAAC CCGGCGGAGT TTGCCAAGTA CAAGCTGGAT 
AAGCCCATCG TTGATATTGG GGGGATCTCC GGCATGTTAT GGATTCATTT GCTAGCAGGT 
CGCGGCCTAA GAACCGCTCC AGAGGGAGCA GCAGGAACGG CGACACAGGG CCAAACCAGA 
GATCTTTACT GCGTAATCGA GTGCGATCGA GTACATAAAG CACGGACTGT GGTACGATCG 
GGTGACCTGC AGTTCGACTG GGACGAGTCG TTTGAGCTCG ACTTGGTGGG CAACAAACAG 
TTAGATGTAC TAGTCTACTC ATGGGACCCG CAGCACAGAC ACAAGCTGTG TTACCGAGGC 
GCAATCTCGT TATCGTCGAT CCTCCGTCAG TCTCCACTTC ATCAACTGGC CTTGAAGGTT 
GAACCAAGAG GTACGATATA CATTCGCATG CGGCACACGG ATCCACTGGC TCTCTACAAG 
AGAAGGGGAC TTCCGAGCCT GAGAGCAGGT TACCCTACGC TCTTCGGCGC TGATTTGGAA 
ACGGTGGTGA ATCGGGAGTC TAAGAATGCT CCCGGAAGTG CACCTGTTCC CATCGTATTA 
AGGCGCTGTG TGGAGGAGGT GGAACGGCGT GGTCTTGATA TAATCGGGTT GTACCGCCTG 
TGCGGCTCGG CTACCAAGAA GCGATTGCTA CGCGAGGCCT TTGAGCGCAA TAGCCGTGCA 
GTGGAATTGA CCCCGGAACA TGTTCCTGAC ATCAACGTCA TCACCGGCGT CCTCAAGGAT 
TACCTTAGGG AGCTGCCCGA GCCGCTGTTC ACGCGCTGTC TTTTCCAGAT GACGGTGGAT 
GCCTTAGCTG TCTGCCTGCC AGATGACCCA GAGGGCAATG CGAAACTGAT GCTTAGCATA 
CTCGACTGCC TACCGCGGGC GAACAGGGCC ACCCTGGTAT TCCTTCTTGA TCACCTCTCG 
CTGGTCGTTT CAAACTCGGA GCGGAACAAG ATGTCAGCCC AGGCGTTGGC CACCGTGATG 
GGCCCACCGC TGATGCTGCA TTCGGCGAGT GCGCAGCCGG GTGCTGACAT CGATCACGCT 
CAGCCGATCG CGGTGCTTAA GTATCTTCTG CAGATCTGGC CGCAGCCGCA GGCGCAGCAT 
CAGCAGATGG CGCAGCACAT GGGCGGCGCT GCGGGGGCAA TGATGGGCGG TTTGGTCACC 
GCCGGGAGCA TGAGCAATAT GGCCGGTGTT GCTTCAGGTC GGCGCGGCGA GTCAACAGGG 
CAGCGTGGAA GCAAAGTCAG TGCGTTGCCA GCGGACAGAC AGCAACTTCT ACTGCAGCAG 
CAGGCGCAGC TCATGGCGGC GGGCAATCTT CTGCGCTCGT CCACTTCGGT AACCAACATA 
CTCTCCCAAG GCCATCCTCA GCTCTCAGCC ACAGCCAACA ATCATCTGTA TCAATCAGTA 
GTGGGTCAGT TAGCTCAATC GCATCGAGCC TTGCAACAAG CGGTGCAACA GCCCTATCAA 
TTGGGGGGCT CAGTGGGCTC AGCAATTCCC GACCCATCGC CACTGCCACT TCCAGGCACA 
CCCTCCCCCG GCAGTAGCTC AGCATCGACG GGCTCAGGCT CCGGATCGGG TAAAAGCACG 
GACACCATCA AGCGCGGTGC TTCGCCTGTT TCCGTTAAGC AAGTCAAGAT CGTCGACCAG 
CCATCCAGCC CATACTCCAT TGTGATGAAG AAACCGCCGT TGCAAAAAGA TGCGCCCGTT 
GAAATCACCA CCCCCACCAC ACAGGCTGAT ACAGAGTCGA CCTTGGGATG CAAGGAAAGC 
AATGGAACAG CTTCTCGAAG GGGAAATGTA GACTTTTATG ATACGCATAA GACGCAGGCA 
AAGAGTGTGG TAAACGAGGA ATCCAGCTAC AGCTCCAAGT ACACTGGCTC GGAGACCAAG 
AAGATCATCC CTGGCAACAG TAGCTACACT CCCAGCAAGG CGAATGCTAG TGGCCTTTCT 
GGCGGCGAAG ACTACAAAGC AATGCGTAAC AAGTCGAGCG CTACATCCAG CTCCAGTTCA 
TCGCAGGCAA CCGTTTTGAG TGCTGGCTCT ACCGCCACAT CAGCCCCGAC CACTTCTTCA 
GACGATTCGG ATGACCTGGT CTCCTACAAG TCATCGGCGT CCACAAATGC CTTGCTGGCC 
CAATCGCAGG CCATGACCAC CAGCCAGCTG ATGTCCAAGT ATCTTAAGCG GGAGCCACGA 
GTACAGTTTA CGCCAATCAA GTCACCGGAA TCTCCCTCGC CACCGGGTAG CGGCGATGGT 
CTGCCGAAGG GTACTTACCA ACTAGTTACG CCTATCTCGG GATCGTCGAG CAAACCGGGT 
GCTACAACTG GAGCTATAAG CAAGTATACC ACCGGCTCAG TGGAATCATC TATTAATGCG 
AACAGCCAGA AGTTGTCATC GCCCTCACGA TTGTGCAACA GCAAGGATAG CAATAGCAGG 
ACTGGAACGG CCAGTAGTAC GACCCCCGCG ACCTCCATGG TTTCTACAGG TCGGCGTTTG 
TTTGACAGTC TCGCCTCTTC ATCGTCCTCG GAAACGGAGA CCAAGACGTA CATAGGTGGC 
ACCACTGCAG CCAGTGGAGC CATTACCACC ACCATCTACA CCAACGACAC CAAGAACTCC 
GGCAGCAGTA GCAGTAAGTC GGGAATAGGA GGAGGGTCTG GAACTGGGTT GGGAGCAGTT 
TCGGGAGCAA GCTCCGAGAC CCGAAGCTTC GGCAGTACGC TATTTGGCAG TAGTGGCTTA 
GGTAATGGCA ATGGTAGTAG CCACAACCAC TCTAGCGCCA GTCCAAGTCC CTTCACCACC 
ACCAACGGCA ACGGTAACCA CAATACCATG CATCTGTACG GCACCTTGCC CAAAAACGGA 
ACTTCCACGG GGGCAGCTCT GTTTGGCGGA TCGGCCAACA GTTCTTCGTA CCACTCTTCG 
GCGAGTGGAA GTGGAGCCGG AACCGCCAGC AGTAGTGGGG TCAGCTCTAT GACTGGCTCC 
ACAAACAGCT ACGACTTCTA CACGAGCACA AGTTCCACTG TGAGCAGTTC GCGCCCCTTT 
GCCAATGGAG GCAACAACTA TCACACACTG GGAACCTATA GGGCTCAGTA CGCCGCCACT 
AACCCCTTCC TGGACGCATT TGATGAAAAG CCGGGCAGCA ACGGGGGTAA TGCCCACGGG 
GAGGAGAAGC TAGGCGCGGA CAAGGGCCAC CACAGAGCGG CCGTTATGGC CTTTCAGTCT 
TCAGGAGACT CGAAAAACGG TAGCGATGAG TACGATGACA TCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651902.2
CDS	48..5615
Translation	MCDSATTGCFLTRSSHRKENGRSVPDVTASPGRAPPGPLPANQMPAMGNQQHHGNQQHHGNQQQHHGNQHSNHRGQSGSLSNAAGVKDPVMLQGDFRKVSGISSEIFRQIEAVENDHDPNTAAALEAVERRGEMIVRVLEPRCMGSKQAVDAAHKLMNKADARHTVQLVEIVKRPGQTLGLYIREGNGADRTDGVFISRIALESAVYNSGCLRVGDEILAVNLVDVTHMSLDDVVIIMSIPRRLVLAIRQRRGNRGTGSPGPPTLSRPEQKPPPVVVIKRDLRDEDLDETDRMPRPRSSRDRRTGDGREMTESRSRLGLGLNNYSPQSEQLDMYYNTRGGGGGAMGEPPNWGYKPPPPPSSVITEQPTKAHAFAPSHAYYQNAGTLESLAEKVHAFYPGQPGGPPVGPSRRMSTGTGNVGLAQQHARFPRSGSDQHLPRVEYSDYSNSLGRHSLLRSSLKPGTTGGAPMQVGVGGTLGRYGRYDQQRAGVSKYGPPSGGAQSLTRRSRPNLDYSSDTEATIGPRPSYYYYNRPAIGSMSRGSGGAGGGVGAASTAALLAGAADLNKFNSLPRERPGTRLQGIRSRMGDRLVDENDGNTSAPEFDVRRGRDLRQRITASPSIFTADEYRAWLRRAPSSSAIAEQMRMTRDMFAQPRAQRFSCSAENIHDALRNTESIYSSRNHILGTGTLDRNMGLTRPISALPVRSMSSQHIGGAGSIRSPSIRRMRQLLELSAGPASPSGSILSTGGHQSPAPTPSATLPRPHRQIDINPAEFAKYKLDKPIVDIGGISGMLWIHLLAGRGLRTAPEGAAGTATQGQTRDLYCVIECDRVHKARTVVRSGDLQFDWDESFELDLVGNKQLDVLVYSWDPQHRHKLCYRGAISLSSILRQSPLHQLALKVEPRGTIYIRMRHTDPLALYKRRGLPSLRAGYPTLFGADLETVVNRESKNAPGSAPVPIVLRRCVEEVERRGLDIIGLYRLCGSATKKRLLREAFERNSRAVELTPEHVPDINVITGVLKDYLRELPEPLFTRCLFQMTVDALAVCLPDDPEGNAKLMLSILDCLPRANRATLVFLLDHLSLVVSNSERNKMSAQALATVMGPPLMLHSASAQPGADIDHAQPIAVLKYLLQIWPQPQAQHQQMAQHMGGAAGAMMGGLVTAGSMSNMAGVASGRRGESTGQRGSKVSALPADRQQLLLQQQAQLMAAGNLLRSSTSVTNILSQGHPQLSATANNHLYQSVVGQLAQSHRALQQAVQQPYQLGGSVGSAIPDPSPLPLPGTPSPGSSSASTGSGSGSGKSTDTIKRGASPVSVKQVKIVDQPSSPYSIVMKKPPLQKDAPVEITTPTTQADTESTLGCKESNGTASRRGNVDFYDTHKTQAKSVVNEESSYSSKYTGSETKKIIPGNSSYTPSKANASGLSGGEDYKAMRNKSSATSSSSSSQATVLSAGSTATSAPTTSSDDSDDLVSYKSSASTNALLAQSQAMTTSQLMSKYLKREPRVQFTPIKSPESPSPPGSGDGLPKGTYQLVTPISGSSSKPGATTGAISKYTTGSVESSINANSQKLSSPSRLCNSKDSNSRTGTASSTTPATSMVSTGRRLFDSLASSSSSETETKTYIGGTTAASGAITTTIYTNDTKNSGSSSSKSGIGGGSGTGLGAVSGASSETRSFGSTLFGSSGLGNGNGSSHNHSSASPSPFTTTNGNGNHNTMHLYGTLPKNGTSTGAALFGGSANSSSYHSSASGSGAGTASSSGVSSMTGSTNSYDFYTSTSSTVSSSRPFANGGNNYHTLGTYRAQYAATNPFLDAFDEKPGSNGGNAHGEEKLGADKGHHRAAVMAFQSSGDSKNGSDEYDDIK

Target ORF information:

RefSeq Version	NM_143645.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143645.2

ORF Insert Sequence:

ATGTGTGATA GTGCGACCAC GGGATGTTTC CTGACCAGAA GCAGCCATCG CAAGGAAAAT 
GGAAGATCAG TTCCTGATGT CACCGCAAGC CCGGGACGGG CCCCGCCCGG ACCACTGCCC 
GCCAACCAGA TGCCTGCGAT GGGCAACCAG CAACACCATG GAAACCAGCA ACATCATGGA 
AACCAGCAGC AACATCATGG CAATCAGCAC AGCAACCATC GAGGTCAGAG CGGAAGTTTG 
TCAAACGCCG CCGGGGTCAA GGACCCGGTG ATGCTACAGG GCGATTTTCG GAAAGTCAGC 
GGAATCAGCT CAGAGATCTT TCGGCAGATA GAAGCCGTCG AGAATGACCA CGACCCAAAC 
ACGGCGGCGG CTTTGGAGGC GGTGGAGAGA CGCGGTGAGA TGATCGTGCG CGTCCTGGAG 
CCGCGTTGCA TGGGCAGCAA ACAGGCGGTG GACGCTGCCC ACAAGCTGAT GAACAAGGCG 
GACGCACGGC ACACCGTGCA GCTGGTAGAA ATAGTCAAGC GGCCGGGCCA GACGCTGGGC 
TTGTACATCC GCGAGGGAAA TGGGGCCGAT CGAACTGATG GCGTCTTTAT TTCGCGGATT 
GCACTGGAGT CGGCTGTCTA CAACAGCGGC TGCTTGCGGG TGGGCGACGA AATCCTTGCG 
GTAAATCTGG TGGACGTGAC ACACATGTCC TTAGACGATG TCGTCATTAT TATGTCAATT 
CCGCGCCGCT TGGTGCTGGC TATACGTCAG CGGCGGGGCA ATAGAGGCAC AGGATCTCCT 
GGACCGCCAA CGCTCTCTAG GCCAGAACAG AAGCCCCCAC CGGTTGTTGT CATCAAGCGA 
GATTTGCGAG ACGAGGATCT GGACGAGACG GACCGGATGC CGAGGCCGCG CTCATCACGT 
GACAGACGTA CAGGAGATGG TCGCGAAATG ACGGAATCTC GTTCTAGGCT AGGTCTGGGT 
CTTAATAATT ACAGCCCGCA GTCCGAGCAG TTGGATATGT ACTACAATAC CCGCGGCGGA 
GGTGGAGGAG CCATGGGTGA GCCTCCAAAC TGGGGCTATA AACCACCACC GCCACCGTCT 
TCGGTCATTA CGGAGCAACC CACGAAAGCA CATGCTTTTG CGCCGTCGCA TGCTTACTAC 
CAAAATGCCG GCACTCTTGA AAGCTTGGCG GAGAAGGTAC ATGCATTCTA TCCTGGACAG 
CCTGGTGGTC CGCCAGTGGG TCCCTCGAGA AGAATGTCAA CGGGAACTGG AAACGTTGGC 
CTCGCTCAGC AACATGCCAG ATTTCCGCGA TCTGGCTCAG ATCAGCATTT ACCTCGCGTA 
GAATATTCGG ACTATTCCAA TTCACTGGGG CGGCATTCCC TTCTGCGATC AAGTTTAAAA 
CCTGGAACGA CCGGTGGAGC TCCAATGCAG GTAGGAGTGG GAGGCACTCT TGGCCGATAC 
GGCCGCTATG ATCAACAGAG AGCCGGTGTA TCCAAGTACG GCCCTCCATC TGGTGGAGCT 
CAATCGCTGA CCCGTCGTTC CAGACCAAAT TTGGACTATT CCAGCGATAC GGAAGCCACA 
ATTGGGCCCA GGCCAAGTTA CTACTACTAT AACAGACCTG CCATCGGCAG TATGTCGAGG 
GGATCAGGTG GAGCAGGCGG AGGAGTTGGC GCAGCTTCAA CGGCTGCCTT GCTGGCTGGA 
GCTGCTGATC TTAACAAATT TAACTCGTTG CCACGAGAGC GCCCCGGGAC GAGACTGCAG 
GGAATTCGTT CCAGAATGGG AGATCGTTTG GTGGACGAAA ATGACGGAAA TACTTCGGCA 
CCCGAGTTCG ATGTACGGAG AGGAAGGGAC TTACGTCAGC GAATTACCGC CAGTCCGTCG 
ATATTTACGG CAGATGAATA CCGCGCCTGG CTCAGAAGGG CGCCTAGCAG TTCCGCAATT 
GCGGAACAAA TGCGAATGAC GCGGGACATG TTTGCCCAGC CACGGGCTCA GCGATTTTCG 
TGTAGCGCTG AGAACATCCA TGATGCACTG AGAAATACGG AAAGCATCTA CTCCAGTAGA 
AACCATATTC TTGGTACGGG CACTCTCGAT CGGAACATGG GTCTTACCCG ACCAATTTCA 
GCACTACCCG TGCGCTCCAT GTCCTCACAG CACATTGGAG GAGCGGGTTC CATTCGTTCG 
CCTAGCATAC GGCGCATGCG ACAGTTACTG GAGCTTTCCG CTGGTCCCGC CAGTCCGAGC 
GGCAGTATCT TGAGCACCGG TGGTCATCAG AGTCCGGCAC CAACTCCAAG CGCGACCCTA 
CCACGCCCGC ACCGCCAAAT CGACATCAAC CCGGCGGAGT TTGCCAAGTA CAAGCTGGAT 
AAGCCCATCG TTGATATTGG GGGGATCTCC GGCATGTTAT GGATTCATTT GCTAGCAGGT 
CGCGGCCTAA GAACCGCTCC AGAGGGAGCA GCAGGAACGG CGACACAGGG CCAAACCAGA 
GATCTTTACT GCGTAATCGA GTGCGATCGA GTACATAAAG CACGGACTGT GGTACGATCG 
GGTGACCTGC AGTTCGACTG GGACGAGTCG TTTGAGCTCG ACTTGGTGGG CAACAAACAG 
TTAGATGTAC TAGTCTACTC ATGGGACCCG CAGCACAGAC ACAAGCTGTG TTACCGAGGC 
GCAATCTCGT TATCGTCGAT CCTCCGTCAG TCTCCACTTC ATCAACTGGC CTTGAAGGTT 
GAACCAAGAG GTACGATATA CATTCGCATG CGGCACACGG ATCCACTGGC TCTCTACAAG 
AGAAGGGGAC TTCCGAGCCT GAGAGCAGGT TACCCTACGC TCTTCGGCGC TGATTTGGAA 
ACGGTGGTGA ATCGGGAGTC TAAGAATGCT CCCGGAAGTG CACCTGTTCC CATCGTATTA 
AGGCGCTGTG TGGAGGAGGT GGAACGGCGT GGTCTTGATA TAATCGGGTT GTACCGCCTG 
TGCGGCTCGG CTACCAAGAA GCGATTGCTA CGCGAGGCCT TTGAGCGCAA TAGCCGTGCA 
GTGGAATTGA CCCCGGAACA TGTTCCTGAC ATCAACGTCA TCACCGGCGT CCTCAAGGAT 
TACCTTAGGG AGCTGCCCGA GCCGCTGTTC ACGCGCTGTC TTTTCCAGAT GACGGTGGAT 
GCCTTAGCTG TCTGCCTGCC AGATGACCCA GAGGGCAATG CGAAACTGAT GCTTAGCATA 
CTCGACTGCC TACCGCGGGC GAACAGGGCC ACCCTGGTAT TCCTTCTTGA TCACCTCTCG 
CTGGTCGTTT CAAACTCGGA GCGGAACAAG ATGTCAGCCC AGGCGTTGGC CACCGTGATG 
GGCCCACCGC TGATGCTGCA TTCGGCGAGT GCGCAGCCGG GTGCTGACAT CGATCACGCT 
CAGCCGATCG CGGTGCTTAA GTATCTTCTG CAGATCTGGC CGCAGCCGCA GGCGCAGCAT 
CAGCAGATGG CGCAGCACAT GGGCGGCGCT GCGGGGGCAA TGATGGGCGG TTTGGTCACC 
GCCGGGAGCA TGAGCAATAT GGCCGGTGTT GCTTCAGGTC GGCGCGGCGA GTCAACAGGG 
CAGCGTGGAA GCAAAGTCAG TGCGTTGCCA GCGGACAGAC AGCAACTTCT ACTGCAGCAG 
CAGGCGCAGC TCATGGCGGC GGGCAATCTT CTGCGCTCGT CCACTTCGGT AACCAACATA 
CTCTCCCAAG GCCATCCTCA GCTCTCAGCC ACAGCCAACA ATCATCTGTA TCAATCAGTA 
GTGGGTCAGT TAGCTCAATC GCATCGAGCC TTGCAACAAG CGGTGCAACA GCCCTATCAA 
TTGGGGGGCT CAGTGGGCTC AGCAATTCCC GACCCATCGC CACTGCCACT TCCAGGCACA 
CCCTCCCCCG GCAGTAGCTC AGCATCGACG GGCTCAGGCT CCGGATCGGG TAAAAGCACG 
GACACCATCA AGCGCGGTGC TTCGCCTGTT TCCGTTAAGC AAGTCAAGAT CGTCGACCAG 
CCATCCAGCC CATACTCCAT TGTGATGAAG AAACCGCCGT TGCAAAAAGA TGCGCCCGTT 
GAAATCACCA CCCCCACCAC ACAGGCTGAT ACAGAGTCGA CCTTGGGATG CAAGGAAAGC 
AATGGAACAG CTTCTCGAAG GGGAAATGTA GACTTTTATG ATACGCATAA GACGCAGGCA 
AAGAGTGTGG TAAACGAGGA ATCCAGCTAC AGCTCCAAGT ACACTGGCTC GGAGACCAAG 
AAGATCATCC CTGGCAACAG TAGCTACACT CCCAGCAAGG CGAATGCTAG TGGCCTTTCT 
GGCGGCGAAG ACTACAAAGC AATGCGTAAC AAGTCGAGCG CTACATCCAG CTCCAGTTCA 
TCGCAGGCAA CCGTTTTGAG TGCTGGCTCT ACCGCCACAT CAGCCCCGAC CACTTCTTCA 
GACGATTCGG ATGACCTGGT CTCCTACAAG TCATCGGCGT CCACAAATGC CTTGCTGGCC 
CAATCGCAGG CCATGACCAC CAGCCAGCTG ATGTCCAAGT ATCTTAAGCG GGAGCCACGA 
GTACAGTTTA CGCCAATCAA GTCACCGGAA TCTCCCTCGC CACCGGGTAG CGGCGATGGT 
CTGCCGAAGG GTACTTACCA ACTAGTTACG CCTATCTCGG GATCGTCGAG CAAACCGGGT 
GCTACAACTG GAGCTATAAG CAAGTATACC ACCGGCTCAG TGGAATCATC TATTAATGCG 
AACAGCCAGA AGTTGTCATC GCCCTCACGA TTGTGCAACA GCAAGGATAG CAATAGCAGG 
ACTGGAACGG CCAGTAGTAC GACCCCCGCG ACCTCCATGG TTTCTACAGG TCGGCGTTTG 
TTTGACAGTC TCGCCTCTTC ATCGTCCTCG GAAACGGAGA CCAAGACGTA CATAGGTGGC 
ACCACTGCAG CCAGTGGAGC CATTACCACC ACCATCTACA CCAACGACAC CAAGAACTCC 
GGCAGCAGTA GCAGTAAGTC GGGAATAGGA GGAGGGTCTG GAACTGGGTT GGGAGCAGTT 
TCGGGAGCAA GCTCCGAGAC CCGAAGCTTC GGCAGTACGC TATTTGGCAG TAGTGGCTTA 
GGTAATGGCA ATGGTAGTAG CCACAACCAC TCTAGCGCCA GTCCAAGTCC CTTCACCACC 
ACCAACGGCA ACGGTAACCA CAATACCATG CATCTGTACG GCACCTTGCC CAAAAACGGA 
ACTTCCACGG GGGCAGCTCT GTTTGGCGGA TCGGCCAACA GTTCTTCGTA CCACTCTTCG 
GCGAGTGGAA GTGGAGCCGG AACCGCCAGC AGTAGTGGGG TCAGCTCTAT GACTGGCTCC 
ACAAACAGCT ACGACTTCTA CACGAGCACA AGTTCCACTG TGAGCAGTTC GCGCCCCTTT 
GCCAATGGAG GCAACAACTA TCACACACTG GGAACCTATA GGGCTCAGTA CGCCGCCACT 
AACCCCTTCC TGGACGCATT TGATGAAAAG CCGGGCAGCA ACGGGGGTAA TGCCCACGGG 
GAGGAGAAGC TAGGCGCGGA CAAGGGCCAC CACAGAGCGG CCGTTATGGC CTTTCAGTCT 
TCAGGAGACT CGAAAAACGG TAGCGATGAG TACGATGACA TCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP100F ( NM_001170315.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163786.1 Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant D, mRNA.

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CloneID	OFa07752
Clone ID Related Accession (Same CDS sequence)	NM_001170315.3
Accession Version	NM_001170315.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 100F, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170315.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTGGA AGAAGAAGTT TACTCGATTG AAAGCAGCTA CTGGAAATTC GCGTGTGCGA 
AGAATGCTTT GTTGTGGACG CAGAAAGGAA AATGGAAGAT CAGTTCCTGA TGTCACCGCA 
AGCCCGGGAC GGGCCCCGCC CGGACCACTG CCCGCCAACC AGATGCCTGC GATGGGCAAC 
CAGCAACACC ATGGAAACCA GCAACATCAT GGAAACCAGC AGCAACATCA TGGCAATCAG 
CACAGCAACC ATCGAGGTCA GAGCGGAAGT TTGTCAAACG CCGCCGGGGT CAAGGACCCG 
GTGATGCTAC AGGGCGATTT TCGGAAAGTC AGCGGAATCA GCTCAGAGAT CTTTCGGCAG 
ATAGAAGCCG TCGAGAATGA CCACGACCCA AACACGGCGG CGGCTTTGGA GGCGGTGGAG 
AGACGCGGTG AGATGATCGT GCGCGTCCTG GAGCCGCGTT GCATGGGCAG CAAACAGGCG 
GTGGACGCTG CCCACAAGCT GATGAACAAG GCGGACGCAC GGCACACCGT GCAGCTGGTA 
GAAATAGTCA AGCGGCCGGG CCAGACGCTG GGCTTGTACA TCCGCGAGGG AAATGGGGCC 
GATCGAACTG ATGGCGTCTT TATTTCGCGG ATTGCACTGG AGTCGGCTGT CTACAACAGC 
GGCTGCTTGC GGGTGGGCGA CGAAATCCTT GCGGTAAATC TGGTGGACGT GACACACATG 
TCCTTAGACG ATGTCGTCAT TATTATGTCA ATTCCGCGCC GCTTGGTGCT GGCTATACGT 
CAGCGGCGGG GCAATAGAGG CACAGGATCT CCTGGACCGC CAACGCTCTC TAGGCCAGAA 
CAGAAGCCCC CACCGGTTGT TGTCATCAAG CGAGATTTGC GAGACGAGGA TCTGGACGAG 
ACGGACCGGA TGCCGAGGCC GCGCTCATCA CGTGACAGAC GTACAGGAGA TGGTCGCGAA 
ATGACGGAAT CTCGTTCTAG GCTAGGTCTG GGTCTTAATA ATTACAGCCC GCAGTCCGAG 
CAGTTGGATA TGTACTACAA TACCCGCGGC GGAGGTGGAG GAGCCATGGG TGAGCCTCCA 
AACTGGGGCT ATAAACCACC ACCGCCACCG TCTTCGGTCA TTACGGAGCA ACCCACGAAA 
GCACATGCTT TTGCGCCGTC GCATGCTTAC TACCAAAATG CCGGCACTCT TGAAAGCTTG 
GCGGAGAAGG TACATGCATT CTATCCTGGA CAGCCTGGTG GTCCGCCAGT GGGTCCCTCG 
AGAAGAATGT CAACGGGAAC TGGAAACGTT GGCCTCGCTC AGCAACATGC CAGATTTCCG 
CGATCTGGCT CAGATCAGCA TTTACCTCGC GTAGAATATT CGGACTATTC CAATTCACTG 
GGGCGGCATT CCCTTCTGCG ATCAAGTTTA AAACCTGGAA CGACCGGTGG AGCTCCAATG 
CAGGTAGGAG TGGGAGGCAC TCTTGGCCGA TACGGCCGCT ATGATCAACA GAGAGCCGGT 
GTATCCAAGT ACGGCCCTCC ATCTGGTGGA GCTCAATCGC TGACCCGTCG TTCCAGACCA 
AATTTGGACT ATTCCAGCGA TACGGAAGCC ACAATTGGGC CCAGGCCAAG TTACTACTAC 
TATAACAGAC CTGCCATCGG CAGTATGTCG AGGGGATCAG GTGGAGCAGG CGGAGGAGTT 
GGCGCAGCTT CAACGGCTGC CTTGCTGGCT GGAGCTGCTG ATCTTAACAA ATTTAACTCG 
TTGCCACGAG AGCGCCCCGG GACGAGACTG CAGGGAATTC GTTCCAGAAT GGGAGATCGT 
TTGGTGGACG AAAATGACGG AAATACTTCG GCACCCGAGT TCGATGTACG GAGAGGAAGG 
GACTTACGTC AGCGAATTAC CGCCAGTCCG TCGATATTTA CGGCAGATGA ATACCGCGCC 
TGGCTCAGAA GGGCGCCTAG CAGTTCCGCA ATTGCGGAAC AAATGCGAAT GACGCGGGAC 
ATGTTTGCCC AGCCACGGGC TCAGCGATTT TCGTGTAGCG CTGAGAACAT CCATGATGCA 
CTGAGAAATA CGGAAAGCAT CTACTCCAGT AGAAACCATA TTCTTGGTAC GGGCACTCTC 
GATCGGAACA TGGGTCTTAC CCGACCAATT TCAGCACTAC CCGTGCGCTC CATGTCCTCA 
CAGCACATTG GAGGAGCGGG TTCCATTCGT TCGCCTAGCA TACGGCGCAT GCGACAGTTA 
CTGGAGCTTT CCGCTGGTCC CGCCAGTCCG AGCGGCAGTA TCTTGAGCAC CGGTGGTCAT 
CAGAGTCCGG CACCAACTCC AAGCGCGACC CTACCACGCC CGCACCGCCA AATCGACATC 
AACCCGGCGG AGTTTGCCAA GTACAAGCTG GATAAGCCCA TCGTTGATAT TGGGGGGATC 
TCCGGCATGT TATGGATTCA TTTGCTAGCA GGTCGCGGCC TAAGAACCGC TCCAGAGGGA 
GCAGCAGGAA CGGCGACACA GGGCCAAACC AGAGATCTTT ACTGCGTAAT CGAGTGCGAT 
CGAGTACATA AAGCACGGAC TGTGGTACGA TCGGGTGACC TGCAGTTCGA CTGGGACGAG 
TCGTTTGAGC TCGACTTGGT GGGCAACAAA CAGTTAGATG TACTAGTCTA CTCATGGGAC 
CCGCAGCACA GACACAAGCT GTGTTACCGA GGCGCAATCT CGTTATCGTC GATCCTCCGT 
CAGTCTCCAC TTCATCAACT GGCCTTGAAG GTTGAACCAA GAGGTACGAT ATACATTCGC 
ATGCGGCACA CGGATCCACT GGCTCTCTAC AAGAGAAGGG GACTTCCGAG CCTGAGAGCA 
GGTTACCCTA CGCTCTTCGG CGCTGATTTG GAAACGGTGG TGAATCGGGA GTCTAAGAAT 
GCTCCCGGAA GTGCACCTGT TCCCATCGTA TTAAGGCGCT GTGTGGAGGA GGTGGAACGG 
CGTGGTCTTG ATATAATCGG GTTGTACCGC CTGTGCGGCT CGGCTACCAA GAAGCGATTG 
CTACGCGAGG CCTTTGAGCG CAATAGCCGT GCAGTGGAAT TGACCCCGGA ACATGTTCCT 
GACATCAACG TCATCACCGG CGTCCTCAAG GATTACCTTA GGGAGCTGCC CGAGCCGCTG 
TTCACGCGCT GTCTTTTCCA GATGACGGTG GATGCCTTAG CTGTCTGCCT GCCAGATGAC 
CCAGAGGGCA ATGCGAAACT GATGCTTAGC ATACTCGACT GCCTACCGCG GGCGAACAGG 
GCCACCCTGG TATTCCTTCT TGATCACCTC TCGCTGGTCG TTTCAAACTC GGAGCGGAAC 
AAGATGTCAG CCCAGGCGTT GGCCACCGTG ATGGGCCCAC CGCTGATGCT GCATTCGGCG 
AGTGCGCAGC CGGGTGCTGA CATCGATCAC GCTCAGCCGA TCGCGGTGCT TAAGTATCTT 
CTGCAGATCT GGCCGCAGCC GCAGGCGCAG CATCAGCAGA TGGCGCAGCA CATGGGCGGC 
GCTGCGGGGG CAATGATGGG CGGTTTGGTC ACCGCCGGGA GCATGAGCAA TATGGCCGGT 
GTTGCTTCAG GTCGGCGCGG CGAGTCAACA GGGCAGCGTG GAAGCAAAGT CAGTGCGTTG 
CCAGCGGACA GACAGCAACT TCTACTGCAG CAGCAGGCGC AGCTCATGGC GGCGGGCAAT 
CTTCTGCGCT CGTCCACTTC GGTAACCAAC ATACTCTCCC AAGGCCATCC TCAGCTCTCA 
GCCACAGCCA ACAATCATCT GTATCAATCA GTAGTGGGTC AGTTAGCTCA ATCGCATCGA 
GCCTTGCAAC AAGCGGTGCA ACAGCCCTAT CAATTGGGGG GCTCAGTGGG CTCAGCAATT 
CCCGACCCAT CGCCACTGCC ACTTCCAGGC ACACCCTCCC CCGGCAGTAG CTCAGCATCG 
ACGGGCTCAG GCTCCGGATC GGGTAAAAGC ACGGACACCA TCAAGCGCGG TGCTTCGCCT 
GTTTCCGTTA AGCAAGTCAA GATCGTCGAC CAGCCATCCA GCCCATACTC CATTGTGATG 
AAGAAACCGC CGTTGCAAAA AGATGCGCCC GTTGAAATCA CCACCCCCAC CACACAGGCT 
GATACAGAGT CGACCTTGGG ATGCAAGGAA AGCAATGGAA CAGCTTCTCG AAGGGGAAAT 
GTAGACTTTT ATGATACGCA TAAGACGCAG GCAAAGAGTG TGGTAAACGA GGAATCCAGC 
TACAGCTCCA AGTACACTGG CTCGGAGACC AAGAAGATCA TCCCTGGCAA CAGTAGCTAC 
ACTCCCAGCA AGGCGAATGC TAGTGGCCTT TCTGGCGGCG AAGACTACAA AGCAATGCGT 
AACAAGTCGA GCGCTACATC CAGCTCCAGT TCATCGCAGG CAACCGTTTT GAGTGCTGGC 
TCTACCGCCA CATCAGCCCC GACCACTTCT TCAGACGATT CGGATGACCT GGTCTCCTAC 
AAGTCATCGG CGTCCACAAA TGCCTTGCTG GCCCAATCGC AGGCCATGAC CACCAGCCAG 
CTGATGTCCA AGTATCTTAA GCGGGAGCCA CGAGTACAGT TTACGCCAAT CAAGTCACCG 
GAATCTCCCT CGCCACCGGG TAGCGGCGAT GGTCTGCCGA AGGGTACTTA CCAACTAGTT 
ACGCCTATCT CGGGATCGTC GAGCAAACCG GGTGCTACAA CTGGAGCTAT AAGCAAGTAT 
ACCACCGGCT CAGTGGAATC ATCTATTAAT GCGAACAGCC AGAAGTTGTC ATCGCCCTCA 
CGATTGTGCA ACAGCAAGGA TAGCAATAGC AGGACTGGAA CGGCCAGTAG TACGACCCCC 
GCGACCTCCA TGGTTTCTAC AGGTCGGCGT TTGTTTGACA GTCTCGCCTC TTCATCGTCC 
TCGGAAACGG AGACCAAGAC GTACATAGGT GGCACCACTG CAGCCAGTGG AGCCATTACC 
ACCACCATCT ACACCAACGA CACCAAGAAC TCCGGCAGCA GTAGCAGTAA GTCGGGAATA 
GGAGGAGGGT CTGGAACTGG GTTGGGAGCA GTTTCGGGAG CAAGCTCCGA GACCCGAAGC 
TTCGGCAGTA CGCTATTTGG CAGTAGTGGC TTAGGTAATG GCAATGGTAG TAGCCACAAC 
CACTCTAGCG CCAGTCCAAG TCCCTTCACC ACCACCAACG GCAACGGTAA CCACAATACC 
ATGCATCTGT ACGGCACCTT GCCCAAAAAC GGAACTTCCA CGGGGGCAGC TCTGTTTGGC 
GGATCGGCCA ACAGTTCTTC GTACCACTCT TCGGCGAGTG GAAGTGGAGC CGGAACCGCC 
AGCAGTAGTG GGGTCAGCTC TATGACTGGC TCCACAAACA GCTACGACTT CTACACGAGC 
ACAAGTTCCA CTGTGAGCAG TTCGCGCCCC TTTGCCAATG GAGGCAACAA CTATCACACA 
CTGGGAACCT ATAGGGCTCA GTACGCCGCC ACTAACCCCT TCCTGGACGC ATTTGATGAA 
AAGCCGGGCA GCAACGGGGG TAATGCCCAC GGGGAGGAGA AGCTAGGCGC GGACAAGGGC 
CACCACAGAG CGGCCGTTAT GGCCTTTCAG TCTTCAGGAG ACTCGAAAAA CGGTAGCGAT 
GAGTACGATG ACATCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163786.1
CDS	1432..7032
Translation	MQWKKKFTRLKAATGNSRVRRMLCCGRRKENGRSVPDVTASPGRAPPGPLPANQMPAMGNQQHHGNQQHHGNQQQHHGNQHSNHRGQSGSLSNAAGVKDPVMLQGDFRKVSGISSEIFRQIEAVENDHDPNTAAALEAVERRGEMIVRVLEPRCMGSKQAVDAAHKLMNKADARHTVQLVEIVKRPGQTLGLYIREGNGADRTDGVFISRIALESAVYNSGCLRVGDEILAVNLVDVTHMSLDDVVIIMSIPRRLVLAIRQRRGNRGTGSPGPPTLSRPEQKPPPVVVIKRDLRDEDLDETDRMPRPRSSRDRRTGDGREMTESRSRLGLGLNNYSPQSEQLDMYYNTRGGGGGAMGEPPNWGYKPPPPPSSVITEQPTKAHAFAPSHAYYQNAGTLESLAEKVHAFYPGQPGGPPVGPSRRMSTGTGNVGLAQQHARFPRSGSDQHLPRVEYSDYSNSLGRHSLLRSSLKPGTTGGAPMQVGVGGTLGRYGRYDQQRAGVSKYGPPSGGAQSLTRRSRPNLDYSSDTEATIGPRPSYYYYNRPAIGSMSRGSGGAGGGVGAASTAALLAGAADLNKFNSLPRERPGTRLQGIRSRMGDRLVDENDGNTSAPEFDVRRGRDLRQRITASPSIFTADEYRAWLRRAPSSSAIAEQMRMTRDMFAQPRAQRFSCSAENIHDALRNTESIYSSRNHILGTGTLDRNMGLTRPISALPVRSMSSQHIGGAGSIRSPSIRRMRQLLELSAGPASPSGSILSTGGHQSPAPTPSATLPRPHRQIDINPAEFAKYKLDKPIVDIGGISGMLWIHLLAGRGLRTAPEGAAGTATQGQTRDLYCVIECDRVHKARTVVRSGDLQFDWDESFELDLVGNKQLDVLVYSWDPQHRHKLCYRGAISLSSILRQSPLHQLALKVEPRGTIYIRMRHTDPLALYKRRGLPSLRAGYPTLFGADLETVVNRESKNAPGSAPVPIVLRRCVEEVERRGLDIIGLYRLCGSATKKRLLREAFERNSRAVELTPEHVPDINVITGVLKDYLRELPEPLFTRCLFQMTVDALAVCLPDDPEGNAKLMLSILDCLPRANRATLVFLLDHLSLVVSNSERNKMSAQALATVMGPPLMLHSASAQPGADIDHAQPIAVLKYLLQIWPQPQAQHQQMAQHMGGAAGAMMGGLVTAGSMSNMAGVASGRRGESTGQRGSKVSALPADRQQLLLQQQAQLMAAGNLLRSSTSVTNILSQGHPQLSATANNHLYQSVVGQLAQSHRALQQAVQQPYQLGGSVGSAIPDPSPLPLPGTPSPGSSSASTGSGSGSGKSTDTIKRGASPVSVKQVKIVDQPSSPYSIVMKKPPLQKDAPVEITTPTTQADTESTLGCKESNGTASRRGNVDFYDTHKTQAKSVVNEESSYSSKYTGSETKKIIPGNSSYTPSKANASGLSGGEDYKAMRNKSSATSSSSSSQATVLSAGSTATSAPTTSSDDSDDLVSYKSSASTNALLAQSQAMTTSQLMSKYLKREPRVQFTPIKSPESPSPPGSGDGLPKGTYQLVTPISGSSSKPGATTGAISKYTTGSVESSINANSQKLSSPSRLCNSKDSNSRTGTASSTTPATSMVSTGRRLFDSLASSSSSETETKTYIGGTTAASGAITTTIYTNDTKNSGSSSSKSGIGGGSGTGLGAVSGASSETRSFGSTLFGSSGLGNGNGSSHNHSSASPSPFTTTNGNGNHNTMHLYGTLPKNGTSTGAALFGGSANSSSYHSSASGSGAGTASSSGVSSMTGSTNSYDFYTSTSSTVSSSRPFANGGNNYHTLGTYRAQYAATNPFLDAFDEKPGSNGGNAHGEEKLGADKGHHRAAVMAFQSSGDSKNGSDEYDDIK

Target ORF information:

RefSeq Version	NM_001170315.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170315.3

ORF Insert Sequence:

ATGCAGTGGA AGAAGAAGTT TACTCGATTG AAAGCAGCTA CTGGAAATTC GCGTGTGCGA 
AGAATGCTTT GTTGTGGACG CAGAAAGGAA AATGGAAGAT CAGTTCCTGA TGTCACCGCA 
AGCCCGGGAC GGGCCCCGCC CGGACCACTG CCCGCCAACC AGATGCCTGC GATGGGCAAC 
CAGCAACACC ATGGAAACCA GCAACATCAT GGAAACCAGC AGCAACATCA TGGCAATCAG 
CACAGCAACC ATCGAGGTCA GAGCGGAAGT TTGTCAAACG CCGCCGGGGT CAAGGACCCG 
GTGATGCTAC AGGGCGATTT TCGGAAAGTC AGCGGAATCA GCTCAGAGAT CTTTCGGCAG 
ATAGAAGCCG TCGAGAATGA CCACGACCCA AACACGGCGG CGGCTTTGGA GGCGGTGGAG 
AGACGCGGTG AGATGATCGT GCGCGTCCTG GAGCCGCGTT GCATGGGCAG CAAACAGGCG 
GTGGACGCTG CCCACAAGCT GATGAACAAG GCGGACGCAC GGCACACCGT GCAGCTGGTA 
GAAATAGTCA AGCGGCCGGG CCAGACGCTG GGCTTGTACA TCCGCGAGGG AAATGGGGCC 
GATCGAACTG ATGGCGTCTT TATTTCGCGG ATTGCACTGG AGTCGGCTGT CTACAACAGC 
GGCTGCTTGC GGGTGGGCGA CGAAATCCTT GCGGTAAATC TGGTGGACGT GACACACATG 
TCCTTAGACG ATGTCGTCAT TATTATGTCA ATTCCGCGCC GCTTGGTGCT GGCTATACGT 
CAGCGGCGGG GCAATAGAGG CACAGGATCT CCTGGACCGC CAACGCTCTC TAGGCCAGAA 
CAGAAGCCCC CACCGGTTGT TGTCATCAAG CGAGATTTGC GAGACGAGGA TCTGGACGAG 
ACGGACCGGA TGCCGAGGCC GCGCTCATCA CGTGACAGAC GTACAGGAGA TGGTCGCGAA 
ATGACGGAAT CTCGTTCTAG GCTAGGTCTG GGTCTTAATA ATTACAGCCC GCAGTCCGAG 
CAGTTGGATA TGTACTACAA TACCCGCGGC GGAGGTGGAG GAGCCATGGG TGAGCCTCCA 
AACTGGGGCT ATAAACCACC ACCGCCACCG TCTTCGGTCA TTACGGAGCA ACCCACGAAA 
GCACATGCTT TTGCGCCGTC GCATGCTTAC TACCAAAATG CCGGCACTCT TGAAAGCTTG 
GCGGAGAAGG TACATGCATT CTATCCTGGA CAGCCTGGTG GTCCGCCAGT GGGTCCCTCG 
AGAAGAATGT CAACGGGAAC TGGAAACGTT GGCCTCGCTC AGCAACATGC CAGATTTCCG 
CGATCTGGCT CAGATCAGCA TTTACCTCGC GTAGAATATT CGGACTATTC CAATTCACTG 
GGGCGGCATT CCCTTCTGCG ATCAAGTTTA AAACCTGGAA CGACCGGTGG AGCTCCAATG 
CAGGTAGGAG TGGGAGGCAC TCTTGGCCGA TACGGCCGCT ATGATCAACA GAGAGCCGGT 
GTATCCAAGT ACGGCCCTCC ATCTGGTGGA GCTCAATCGC TGACCCGTCG TTCCAGACCA 
AATTTGGACT ATTCCAGCGA TACGGAAGCC ACAATTGGGC CCAGGCCAAG TTACTACTAC 
TATAACAGAC CTGCCATCGG CAGTATGTCG AGGGGATCAG GTGGAGCAGG CGGAGGAGTT 
GGCGCAGCTT CAACGGCTGC CTTGCTGGCT GGAGCTGCTG ATCTTAACAA ATTTAACTCG 
TTGCCACGAG AGCGCCCCGG GACGAGACTG CAGGGAATTC GTTCCAGAAT GGGAGATCGT 
TTGGTGGACG AAAATGACGG AAATACTTCG GCACCCGAGT TCGATGTACG GAGAGGAAGG 
GACTTACGTC AGCGAATTAC CGCCAGTCCG TCGATATTTA CGGCAGATGA ATACCGCGCC 
TGGCTCAGAA GGGCGCCTAG CAGTTCCGCA ATTGCGGAAC AAATGCGAAT GACGCGGGAC 
ATGTTTGCCC AGCCACGGGC TCAGCGATTT TCGTGTAGCG CTGAGAACAT CCATGATGCA 
CTGAGAAATA CGGAAAGCAT CTACTCCAGT AGAAACCATA TTCTTGGTAC GGGCACTCTC 
GATCGGAACA TGGGTCTTAC CCGACCAATT TCAGCACTAC CCGTGCGCTC CATGTCCTCA 
CAGCACATTG GAGGAGCGGG TTCCATTCGT TCGCCTAGCA TACGGCGCAT GCGACAGTTA 
CTGGAGCTTT CCGCTGGTCC CGCCAGTCCG AGCGGCAGTA TCTTGAGCAC CGGTGGTCAT 
CAGAGTCCGG CACCAACTCC AAGCGCGACC CTACCACGCC CGCACCGCCA AATCGACATC 
AACCCGGCGG AGTTTGCCAA GTACAAGCTG GATAAGCCCA TCGTTGATAT TGGGGGGATC 
TCCGGCATGT TATGGATTCA TTTGCTAGCA GGTCGCGGCC TAAGAACCGC TCCAGAGGGA 
GCAGCAGGAA CGGCGACACA GGGCCAAACC AGAGATCTTT ACTGCGTAAT CGAGTGCGAT 
CGAGTACATA AAGCACGGAC TGTGGTACGA TCGGGTGACC TGCAGTTCGA CTGGGACGAG 
TCGTTTGAGC TCGACTTGGT GGGCAACAAA CAGTTAGATG TACTAGTCTA CTCATGGGAC 
CCGCAGCACA GACACAAGCT GTGTTACCGA GGCGCAATCT CGTTATCGTC GATCCTCCGT 
CAGTCTCCAC TTCATCAACT GGCCTTGAAG GTTGAACCAA GAGGTACGAT ATACATTCGC 
ATGCGGCACA CGGATCCACT GGCTCTCTAC AAGAGAAGGG GACTTCCGAG CCTGAGAGCA 
GGTTACCCTA CGCTCTTCGG CGCTGATTTG GAAACGGTGG TGAATCGGGA GTCTAAGAAT 
GCTCCCGGAA GTGCACCTGT TCCCATCGTA TTAAGGCGCT GTGTGGAGGA GGTGGAACGG 
CGTGGTCTTG ATATAATCGG GTTGTACCGC CTGTGCGGCT CGGCTACCAA GAAGCGATTG 
CTACGCGAGG CCTTTGAGCG CAATAGCCGT GCAGTGGAAT TGACCCCGGA ACATGTTCCT 
GACATCAACG TCATCACCGG CGTCCTCAAG GATTACCTTA GGGAGCTGCC CGAGCCGCTG 
TTCACGCGCT GTCTTTTCCA GATGACGGTG GATGCCTTAG CTGTCTGCCT GCCAGATGAC 
CCAGAGGGCA ATGCGAAACT GATGCTTAGC ATACTCGACT GCCTACCGCG GGCGAACAGG 
GCCACCCTGG TATTCCTTCT TGATCACCTC TCGCTGGTCG TTTCAAACTC GGAGCGGAAC 
AAGATGTCAG CCCAGGCGTT GGCCACCGTG ATGGGCCCAC CGCTGATGCT GCATTCGGCG 
AGTGCGCAGC CGGGTGCTGA CATCGATCAC GCTCAGCCGA TCGCGGTGCT TAAGTATCTT 
CTGCAGATCT GGCCGCAGCC GCAGGCGCAG CATCAGCAGA TGGCGCAGCA CATGGGCGGC 
GCTGCGGGGG CAATGATGGG CGGTTTGGTC ACCGCCGGGA GCATGAGCAA TATGGCCGGT 
GTTGCTTCAG GTCGGCGCGG CGAGTCAACA GGGCAGCGTG GAAGCAAAGT CAGTGCGTTG 
CCAGCGGACA GACAGCAACT TCTACTGCAG CAGCAGGCGC AGCTCATGGC GGCGGGCAAT 
CTTCTGCGCT CGTCCACTTC GGTAACCAAC ATACTCTCCC AAGGCCATCC TCAGCTCTCA 
GCCACAGCCA ACAATCATCT GTATCAATCA GTAGTGGGTC AGTTAGCTCA ATCGCATCGA 
GCCTTGCAAC AAGCGGTGCA ACAGCCCTAT CAATTGGGGG GCTCAGTGGG CTCAGCAATT 
CCCGACCCAT CGCCACTGCC ACTTCCAGGC ACACCCTCCC CCGGCAGTAG CTCAGCATCG 
ACGGGCTCAG GCTCCGGATC GGGTAAAAGC ACGGACACCA TCAAGCGCGG TGCTTCGCCT 
GTTTCCGTTA AGCAAGTCAA GATCGTCGAC CAGCCATCCA GCCCATACTC CATTGTGATG 
AAGAAACCGC CGTTGCAAAA AGATGCGCCC GTTGAAATCA CCACCCCCAC CACACAGGCT 
GATACAGAGT CGACCTTGGG ATGCAAGGAA AGCAATGGAA CAGCTTCTCG AAGGGGAAAT 
GTAGACTTTT ATGATACGCA TAAGACGCAG GCAAAGAGTG TGGTAAACGA GGAATCCAGC 
TACAGCTCCA AGTACACTGG CTCGGAGACC AAGAAGATCA TCCCTGGCAA CAGTAGCTAC 
ACTCCCAGCA AGGCGAATGC TAGTGGCCTT TCTGGCGGCG AAGACTACAA AGCAATGCGT 
AACAAGTCGA GCGCTACATC CAGCTCCAGT TCATCGCAGG CAACCGTTTT GAGTGCTGGC 
TCTACCGCCA CATCAGCCCC GACCACTTCT TCAGACGATT CGGATGACCT GGTCTCCTAC 
AAGTCATCGG CGTCCACAAA TGCCTTGCTG GCCCAATCGC AGGCCATGAC CACCAGCCAG 
CTGATGTCCA AGTATCTTAA GCGGGAGCCA CGAGTACAGT TTACGCCAAT CAAGTCACCG 
GAATCTCCCT CGCCACCGGG TAGCGGCGAT GGTCTGCCGA AGGGTACTTA CCAACTAGTT 
ACGCCTATCT CGGGATCGTC GAGCAAACCG GGTGCTACAA CTGGAGCTAT AAGCAAGTAT 
ACCACCGGCT CAGTGGAATC ATCTATTAAT GCGAACAGCC AGAAGTTGTC ATCGCCCTCA 
CGATTGTGCA ACAGCAAGGA TAGCAATAGC AGGACTGGAA CGGCCAGTAG TACGACCCCC 
GCGACCTCCA TGGTTTCTAC AGGTCGGCGT TTGTTTGACA GTCTCGCCTC TTCATCGTCC 
TCGGAAACGG AGACCAAGAC GTACATAGGT GGCACCACTG CAGCCAGTGG AGCCATTACC 
ACCACCATCT ACACCAACGA CACCAAGAAC TCCGGCAGCA GTAGCAGTAA GTCGGGAATA 
GGAGGAGGGT CTGGAACTGG GTTGGGAGCA GTTTCGGGAG CAAGCTCCGA GACCCGAAGC 
TTCGGCAGTA CGCTATTTGG CAGTAGTGGC TTAGGTAATG GCAATGGTAG TAGCCACAAC 
CACTCTAGCG CCAGTCCAAG TCCCTTCACC ACCACCAACG GCAACGGTAA CCACAATACC 
ATGCATCTGT ACGGCACCTT GCCCAAAAAC GGAACTTCCA CGGGGGCAGC TCTGTTTGGC 
GGATCGGCCA ACAGTTCTTC GTACCACTCT TCGGCGAGTG GAAGTGGAGC CGGAACCGCC 
AGCAGTAGTG GGGTCAGCTC TATGACTGGC TCCACAAACA GCTACGACTT CTACACGAGC 
ACAAGTTCCA CTGTGAGCAG TTCGCGCCCC TTTGCCAATG GAGGCAACAA CTATCACACA 
CTGGGAACCT ATAGGGCTCA GTACGCCGCC ACTAACCCCT TCCTGGACGC ATTTGATGAA 
AAGCCGGGCA GCAACGGGGG TAATGCCCAC GGGGAGGAGA AGCTAGGCGC GGACAAGGGC 
CACCACAGAG CGGCCGTTAT GGCCTTTCAG TCTTCAGGAG ACTCGAAAAA CGGTAGCGAT 
GAGTACGATG ACATCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP100F ( NM_001260465.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247394.1 Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant H, mRNA.

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CloneID	OFa07754
Clone ID Related Accession (Same CDS sequence)	NM_001260465.1
Accession Version	NM_001260465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 100F, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTGATA GTGCGACCAC GGGATGTTTC CTGACCAGAA GCAGCCATCG CAAGGAAAAT 
GGAAGATCAG TTCCTGATGT CACCGCAAGC CCGGGACGGG CCCCGCCCGG ACCACTGCCC 
GCCAACCAGA TGCCTGCGAT GGGCAACCAG CAACACCATG GAAACCAGCA ACATCATGGA 
AACCAGCAGC AACATCATGG CAATCAGCAC AGCAACCATC GAGGTCAGAG CGGAAGTTTG 
TCAAACGCCG CCGGGGTCAA GGACCCGGTG ATGCTACAGG GCGATTTTCG GAAAGTCAGC 
GGAATCAGCT CAGAGATCTT TCGGCAGATA GAAGCCGTCG AGAATGACCA CGACCCAAAC 
ACGGCGGCGG CTTTGGAGGC GGTGGAGAGA CGCGGTGAGA TGATCGTGCG CGTCCTGGAG 
CCGCGTTGCA TGGGCAGCAA ACAGGCGGTG GACGCTGCCC ACAAGCTGAT GAACAAGGCG 
GACGCACGGC ACACCGTGCA GCTGGTAGAA ATAGTCAAGC GGCCGGGCCA GACGCTGGGC 
TTGTACATCC GCGAGGGAAA TGGGGCCGAT CGAACTGATG GCGTCTTTAT TTCGCGGATT 
GCACTGGAGT CGGCTGTCTA CAACAGCGGC TGCTTGCGGG TGGGCGACGA AATCCTTGCG 
GTAAATCTGG TGGACGTGAC ACACATGTCC TTAGACGATG TCGTCATTAT TATGTCAATT 
CCGCGCCGCT TGGTGCTGGC TATACGTCAG CGGCGGGGCA ATAGAGGCAC AGGATCTCCT 
GGACCGCCAA CGCTCTCTAG GCCAGAACAG AAGCCCCCAC CGGTTGTTGT CATCAAGCGA 
GATTTGCGAG ACGAGGATCT GGACGAGACG GACCGGATGC CGAGGCCGCG CTCATCACGT 
GACAGACGTA CAGGTAGAGA TGGTCGCGAA ATGACGGAAT CTCGTTCTAG GCTAGGTCTG 
GGTCTTAATA ATTACAGCCC GCAGTCCGAG CAGTTGGATA TGTACTACAA TACCCGCGGC 
GGAGGTGGAG GAGCCATGGG TGAGCCTCCA AACTGGGGCT ATAAACCACC ACCGCCACCG 
TCTTCGGTCA TTACGGAGCA ACCCACGAAA GCACATGCTT TTGCGCCGTC GCATGCTTAC 
TACCAAAATG CCGGCACTCT TGAAAGCTTG GCGGAGAAGG TACATGCATT CTATCCTGGA 
CAGCCTGGTG GTCCGCCAGT GGGTCCCTCG AGAAGAATGT CAACGGGAAC TGGAAACGTT 
GGCCTCGCTC AGCAACATGC CAGATTTCCG CGATCTGGCT CAGATCAGCA TTTACCTCGC 
GTAGAATATT CGGACTATTC CAATTCACTG GGGCGGCATT CCCTTCTGCG ATCAAGTTTA 
AAACCTGGAA CGACCGGTGG AGCTCCAATG CAGGTAGGAG TGGGAGGCAC TCTTGGCCGA 
TACGGCCGCT ATGATCAACA GAGAGCCGGT GTATCCAAGT ACGGCCCTCC ATCTGGTGGA 
GCTCAATCGC TGACCCGTCG TTCCAGACCA AATTTGGACT ATTCCAGCGA TACGGAAGCC 
ACAATTGGGC CCAGGCCAAG TTACTACTAC TATAACAGAC CTGCCATCGG CAGTATGTCG 
AGGGGATCAG GTGGAGCAGG CGGAGGAGTT GGCGCAGCTT CAACGGCTGC CTTGCTGGCT 
GGAGCTGCTG ATCTTAACAA ATTTAACTCG TTGCCACGAG AGCGCCCCGG GACGAGACTG 
CAGGGAATTC GTTCCAGAAT GGGAGATCGT TTGGTGGACG AAAATGACGG AAATACTTCG 
GCACCCGAGT TCGATGTACG GAGAGGAAGG GACTTACGTC AGCGAATTAC CGCCAGTCCG 
TCGATATTTA CGGCAGATGA ATACCGCGCC TGGCTCAGAA GGGCGCCTAG CAGTTCCGCA 
ATTGCGGAAC AAATGCGAAT GACGCGGGAC ATGTTTGCCC AGCCACGGGC TCAGCGATTT 
TCGTGTAGCG CTGAGAACAT CCATGATGCA CTGAGAAATA CGGAAAGCAT CTACTCCAGT 
AGAAACCATA TTCTTGGTAC GGGCACTCTC GATCGGAACA TGGGTCTTAC CCGACCAATT 
TCAGCACTAC CCGTGCGCTC CATGTCCTCA CAGCACATTG GAGGAGCGGG TTCCATTCGT 
TCGCCTAGCA TACGGCGCAT GCGACAGTTA CTGGAGCTTT CCGCTGGTCC CGCCAGTCCG 
AGCGGCAGTA TCTTGAGCAC CGGTGGTCAT CAGAGTCCGG CACCAACTCC AAGCGCGACC 
CTACCACGCC CGCACCGCCA AATCGACATC AACCCGGCGG AGTTTGCCAA GTACAAGCTG 
GATAAGCCCA TCGTTGATAT TGGGGGGATC TCCGGCATGT TATGGATTCA TTTGCTAGCA 
GGTCGCGGCC TAAGAACCGC TCCAGAGGGA GCAGCAGGAA CGGCGACACA GGGCCAAACC 
AGAGATCTTT ACTGCGTAAT CGAGTGCGAT CGAGTACATA AAGCACGGAC TGTGGTACGA 
TCGGGTGACC TGCAGTTCGA CTGGGACGAG TCGTTTGAGC TCGACTTGGT GGGCAACAAA 
CAGTTAGATG TACTAGTCTA CTCATGGGAC CCGCAGCACA GACACAAGCT GTGTTACCGA 
GGCGCAATCT CGTTATCGTC GATCCTCCGT CAGTCTCCAC TTCATCAACT GGCCTTGAAG 
GTTGAACCAA GAGGTACGAT ATACATTCGC ATGCGGCACA CGGATCCACT GGCTCTCTAC 
AAGAGAAGGG GACTTCCGAG CCTGAGAGCA GGTTACCCTA CGCTCTTCGG CGCTGATTTG 
GAAACGGTGG TGAATCGGGA GTCTAAGAAT GCTCCCGGAA GTGCACCTGT TCCCATCGTA 
TTAAGGCGCT GTGTGGAGGA GGTGGAACGG CGTGGTCTTG ATATAATCGG GTTGTACCGC 
CTGTGCGGCT CGGCTACCAA GAAGCGATTG CTACGCGAGG CCTTTGAGCG CAATAGCCGT 
GCAGTGGAAT TGACCCCGGA ACATGTTCCT GACATCAACG TCATCACCGG CGTCCTCAAG 
GATTACCTTA GGGAGCTGCC CGAGCCGCTG TTCACGCGCT GTCTTTTCCA GATGACGGTG 
GATGCCTTAG CTGTCTGCCT GCCAGATGAC CCAGAGGGCA ATGCGAAACT GATGCTTAGC 
ATACTCGACT GCCTACCGCG GGCGAACAGG GCCACCCTGG TATTCCTTCT TGATCACCTC 
TCGCTGGTCG TTTCAAACTC GGAGCGGAAC AAGATGTCAG CCCAGGCGTT GGCCACCGTG 
ATGGGCCCAC CGCTGATGCT GCATTCGGCG AGTGCGCAGC CGGGTGCTGA CATCGATCAC 
GCTCAGCCGA TCGCGGTGCT TAAGTATCTT CTGCAGATCT GGCCGCAGCC GCAGGCGCAG 
CATCAGCAGA TGGCGCAGCA CATGGGCGGC GCTGCGGGGG CAATGATGGG CGGTTTGGTC 
ACCGCCGGGA GCATGAGCAA TATGGCCGGT GTTGCTTCAG GTCGGCGCGG CGAGTCAACA 
GGGCAGCGTG GAAGCAAAGT CAGTGCGTTG CCAGCGGACA GACAGCAACT TCTACTGCAG 
CAGCAGGCGC AGCTCATGGC GGCGGGCAAT CTTCTGCGCT CGTCCACTTC GGTAACCAAC 
ATACTCTCCC AAGGCCATCC TCAGCTCTCA GCCACAGCCA ACAATCATCT GTATCAATCA 
GTAGTGGGTC AGTTAGCTCA ATCGCATCGA GCCTTGCAAC AAGCGGTGCA ACAGCCCTAT 
CAATTGGGGG GCTCAGTGGG CTCAGCAATT CCCGACCCAT CGCCACTGCC ACTTCCAGGC 
ACACCCTCCC CCGGCAGTAG CTCAGCATCG ACGGGCTCAG GCTCCGGATC GGGTAAAAGC 
ACGGACACCA TCAAGCGCGG TGCTTCGCCT GTTTCCGTTA AGCAAGTCAA GATCGTCGAC 
CAGCCATCCA GCCCATACTC CATTGTGATG AAGAAACCGC CGTTGCAAAA AGATGCGCCC 
GTTGAAATCA CCACCCCCAC CACACAGGCT GATACAGAGT CGACCTTGGG ATGCAAGGAA 
AGCAATGGAA CAGCTTCTCG AAGGGGAAAT GTAGACTTTT ATGATACGCA TAAGACGCAG 
GCAAAGAGTG TGGTAAACGA GGAATCCAGC TACAGCTCCA AGTACACTGG CTCGGAGACC 
AAGAAGATCA TCCCTGGCAA CAGTAGCTAC ACTCCCAGCA AGGCGAATGC TAGTGGCCTT 
TCTGGCGGCG AAGACTACAA AGCAATGCGT AACAAGTCGA GCGCTACATC CAGCTCCAGT 
TCATCGCAGG CAACCGTTTT GAGTGCTGGC TCTACCGCCA CATCAGCCCC GACCACTTCT 
TCAGACGATT CGGATGACCT GGTCTCCTAC AAGTCATCGG CGTCCACAAA TGCCTTGCTG 
GCCCAATCGC AGGCCATGAC CACCAGCCAG CTGATGTCCA AGTATCTTAA GCGGGAGCCA 
CGAGTACAGT TTACGCCAAT CAAGTCACCG GAATCTCCCT CGCCACCGGG TAGCGGCGAT 
GGTCTGCCGA AGGGTACTTA CCAACTAGTT ACGCCTATCT CGGGATCGTC GAGCAAACCG 
GGTGCTACAA CTGGAGCTAT AAGCAAGTAT ACCACCGGCT CAGTGGAATC ATCTATTAAT 
GCGAACAGCC AGAAGTTGTC ATCGCCCTCA CGATTGTGCA ACAGCAAGGA TAGCAATAGC 
AGGACTGGAA CGGCCAGTAG TACGACCCCC GCGACCTCCA TGGTTTCTAC AGGTCGGCGT 
TTGTTTGACA GTCTCGCCTC TTCATCGTCC TCGGAAACGG AGACCAAGAC GTACATAGGT 
GGCACCACTG CAGCCAGTGG AGCCATTACC ACCACCATCT ACACCAACGA CACCAAGAAC 
TCCGGCAGCA GTAGCAGTAA GTCGGGAATA GGAGGAGGGT CTGGAACTGG GTTGGGAGCA 
GTTTCGGGAG CAAGCTCCGA GACCCGAAGC TTCGGCAGTA CGCTATTTGG CAGTAGTGGC 
TTAGGTAATG GCAATGGTAG TAGCCACAAC CACTCTAGCG CCAGTCCAAG TCCCTTCACC 
ACCACCAACG GCAACGGTAA CCACAATACC ATGCATCTGT ACGGCACCTT GCCCAAAAAC 
GGAACTTCCA CGGGGGCAGC TCTGTTTGGC GGATCGGCCA ACAGTTCTTC GTACCACTCT 
TCGGCGAGTG GAAGTGGAGC CGGAACCGCC AGCAGTAGTG GGGTCAGCTC TATGACTGGC 
TCCACAAACA GCTACGACTT CTACACGAGC ACAAGTTCCA CTGTGAGCAG TTCGCGCCCC 
TTTGCCAATG GAGGCAACAA CTATCACACA CTGGGAACCT ATAGGGCTCA GTACGCCGCC 
ACTAACCCCT TCCTGGACGC ATTTGATGAA AAGCCGGGCA GCAACGGGGG TAATGCCCAC 
GGGGAGGAGA AGCTAGGCGC GGACAAGGGC CACCACAGAG CGGCCGTTAT GGCCTTTCAG 
TCTTCAGGAG ACTCGAAAAA CGGTAGCGAT GAGTACGATG ACATCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247394.1
CDS	790..6360
Translation	MCDSATTGCFLTRSSHRKENGRSVPDVTASPGRAPPGPLPANQMPAMGNQQHHGNQQHHGNQQQHHGNQHSNHRGQSGSLSNAAGVKDPVMLQGDFRKVSGISSEIFRQIEAVENDHDPNTAAALEAVERRGEMIVRVLEPRCMGSKQAVDAAHKLMNKADARHTVQLVEIVKRPGQTLGLYIREGNGADRTDGVFISRIALESAVYNSGCLRVGDEILAVNLVDVTHMSLDDVVIIMSIPRRLVLAIRQRRGNRGTGSPGPPTLSRPEQKPPPVVVIKRDLRDEDLDETDRMPRPRSSRDRRTGRDGREMTESRSRLGLGLNNYSPQSEQLDMYYNTRGGGGGAMGEPPNWGYKPPPPPSSVITEQPTKAHAFAPSHAYYQNAGTLESLAEKVHAFYPGQPGGPPVGPSRRMSTGTGNVGLAQQHARFPRSGSDQHLPRVEYSDYSNSLGRHSLLRSSLKPGTTGGAPMQVGVGGTLGRYGRYDQQRAGVSKYGPPSGGAQSLTRRSRPNLDYSSDTEATIGPRPSYYYYNRPAIGSMSRGSGGAGGGVGAASTAALLAGAADLNKFNSLPRERPGTRLQGIRSRMGDRLVDENDGNTSAPEFDVRRGRDLRQRITASPSIFTADEYRAWLRRAPSSSAIAEQMRMTRDMFAQPRAQRFSCSAENIHDALRNTESIYSSRNHILGTGTLDRNMGLTRPISALPVRSMSSQHIGGAGSIRSPSIRRMRQLLELSAGPASPSGSILSTGGHQSPAPTPSATLPRPHRQIDINPAEFAKYKLDKPIVDIGGISGMLWIHLLAGRGLRTAPEGAAGTATQGQTRDLYCVIECDRVHKARTVVRSGDLQFDWDESFELDLVGNKQLDVLVYSWDPQHRHKLCYRGAISLSSILRQSPLHQLALKVEPRGTIYIRMRHTDPLALYKRRGLPSLRAGYPTLFGADLETVVNRESKNAPGSAPVPIVLRRCVEEVERRGLDIIGLYRLCGSATKKRLLREAFERNSRAVELTPEHVPDINVITGVLKDYLRELPEPLFTRCLFQMTVDALAVCLPDDPEGNAKLMLSILDCLPRANRATLVFLLDHLSLVVSNSERNKMSAQALATVMGPPLMLHSASAQPGADIDHAQPIAVLKYLLQIWPQPQAQHQQMAQHMGGAAGAMMGGLVTAGSMSNMAGVASGRRGESTGQRGSKVSALPADRQQLLLQQQAQLMAAGNLLRSSTSVTNILSQGHPQLSATANNHLYQSVVGQLAQSHRALQQAVQQPYQLGGSVGSAIPDPSPLPLPGTPSPGSSSASTGSGSGSGKSTDTIKRGASPVSVKQVKIVDQPSSPYSIVMKKPPLQKDAPVEITTPTTQADTESTLGCKESNGTASRRGNVDFYDTHKTQAKSVVNEESSYSSKYTGSETKKIIPGNSSYTPSKANASGLSGGEDYKAMRNKSSATSSSSSSQATVLSAGSTATSAPTTSSDDSDDLVSYKSSASTNALLAQSQAMTTSQLMSKYLKREPRVQFTPIKSPESPSPPGSGDGLPKGTYQLVTPISGSSSKPGATTGAISKYTTGSVESSINANSQKLSSPSRLCNSKDSNSRTGTASSTTPATSMVSTGRRLFDSLASSSSSETETKTYIGGTTAASGAITTTIYTNDTKNSGSSSSKSGIGGGSGTGLGAVSGASSETRSFGSTLFGSSGLGNGNGSSHNHSSASPSPFTTTNGNGNHNTMHLYGTLPKNGTSTGAALFGGSANSSSYHSSASGSGAGTASSSGVSSMTGSTNSYDFYTSTSSTVSSSRPFANGGNNYHTLGTYRAQYAATNPFLDAFDEKPGSNGGNAHGEEKLGADKGHHRAAVMAFQSSGDSKNGSDEYDDIK

Target ORF information:

RefSeq Version	NM_001260465.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260465.1

ORF Insert Sequence:

ATGTGTGATA GTGCGACCAC GGGATGTTTC CTGACCAGAA GCAGCCATCG CAAGGAAAAT 
GGAAGATCAG TTCCTGATGT CACCGCAAGC CCGGGACGGG CCCCGCCCGG ACCACTGCCC 
GCCAACCAGA TGCCTGCGAT GGGCAACCAG CAACACCATG GAAACCAGCA ACATCATGGA 
AACCAGCAGC AACATCATGG CAATCAGCAC AGCAACCATC GAGGTCAGAG CGGAAGTTTG 
TCAAACGCCG CCGGGGTCAA GGACCCGGTG ATGCTACAGG GCGATTTTCG GAAAGTCAGC 
GGAATCAGCT CAGAGATCTT TCGGCAGATA GAAGCCGTCG AGAATGACCA CGACCCAAAC 
ACGGCGGCGG CTTTGGAGGC GGTGGAGAGA CGCGGTGAGA TGATCGTGCG CGTCCTGGAG 
CCGCGTTGCA TGGGCAGCAA ACAGGCGGTG GACGCTGCCC ACAAGCTGAT GAACAAGGCG 
GACGCACGGC ACACCGTGCA GCTGGTAGAA ATAGTCAAGC GGCCGGGCCA GACGCTGGGC 
TTGTACATCC GCGAGGGAAA TGGGGCCGAT CGAACTGATG GCGTCTTTAT TTCGCGGATT 
GCACTGGAGT CGGCTGTCTA CAACAGCGGC TGCTTGCGGG TGGGCGACGA AATCCTTGCG 
GTAAATCTGG TGGACGTGAC ACACATGTCC TTAGACGATG TCGTCATTAT TATGTCAATT 
CCGCGCCGCT TGGTGCTGGC TATACGTCAG CGGCGGGGCA ATAGAGGCAC AGGATCTCCT 
GGACCGCCAA CGCTCTCTAG GCCAGAACAG AAGCCCCCAC CGGTTGTTGT CATCAAGCGA 
GATTTGCGAG ACGAGGATCT GGACGAGACG GACCGGATGC CGAGGCCGCG CTCATCACGT 
GACAGACGTA CAGGTAGAGA TGGTCGCGAA ATGACGGAAT CTCGTTCTAG GCTAGGTCTG 
GGTCTTAATA ATTACAGCCC GCAGTCCGAG CAGTTGGATA TGTACTACAA TACCCGCGGC 
GGAGGTGGAG GAGCCATGGG TGAGCCTCCA AACTGGGGCT ATAAACCACC ACCGCCACCG 
TCTTCGGTCA TTACGGAGCA ACCCACGAAA GCACATGCTT TTGCGCCGTC GCATGCTTAC 
TACCAAAATG CCGGCACTCT TGAAAGCTTG GCGGAGAAGG TACATGCATT CTATCCTGGA 
CAGCCTGGTG GTCCGCCAGT GGGTCCCTCG AGAAGAATGT CAACGGGAAC TGGAAACGTT 
GGCCTCGCTC AGCAACATGC CAGATTTCCG CGATCTGGCT CAGATCAGCA TTTACCTCGC 
GTAGAATATT CGGACTATTC CAATTCACTG GGGCGGCATT CCCTTCTGCG ATCAAGTTTA 
AAACCTGGAA CGACCGGTGG AGCTCCAATG CAGGTAGGAG TGGGAGGCAC TCTTGGCCGA 
TACGGCCGCT ATGATCAACA GAGAGCCGGT GTATCCAAGT ACGGCCCTCC ATCTGGTGGA 
GCTCAATCGC TGACCCGTCG TTCCAGACCA AATTTGGACT ATTCCAGCGA TACGGAAGCC 
ACAATTGGGC CCAGGCCAAG TTACTACTAC TATAACAGAC CTGCCATCGG CAGTATGTCG 
AGGGGATCAG GTGGAGCAGG CGGAGGAGTT GGCGCAGCTT CAACGGCTGC CTTGCTGGCT 
GGAGCTGCTG ATCTTAACAA ATTTAACTCG TTGCCACGAG AGCGCCCCGG GACGAGACTG 
CAGGGAATTC GTTCCAGAAT GGGAGATCGT TTGGTGGACG AAAATGACGG AAATACTTCG 
GCACCCGAGT TCGATGTACG GAGAGGAAGG GACTTACGTC AGCGAATTAC CGCCAGTCCG 
TCGATATTTA CGGCAGATGA ATACCGCGCC TGGCTCAGAA GGGCGCCTAG CAGTTCCGCA 
ATTGCGGAAC AAATGCGAAT GACGCGGGAC ATGTTTGCCC AGCCACGGGC TCAGCGATTT 
TCGTGTAGCG CTGAGAACAT CCATGATGCA CTGAGAAATA CGGAAAGCAT CTACTCCAGT 
AGAAACCATA TTCTTGGTAC GGGCACTCTC GATCGGAACA TGGGTCTTAC CCGACCAATT 
TCAGCACTAC CCGTGCGCTC CATGTCCTCA CAGCACATTG GAGGAGCGGG TTCCATTCGT 
TCGCCTAGCA TACGGCGCAT GCGACAGTTA CTGGAGCTTT CCGCTGGTCC CGCCAGTCCG 
AGCGGCAGTA TCTTGAGCAC CGGTGGTCAT CAGAGTCCGG CACCAACTCC AAGCGCGACC 
CTACCACGCC CGCACCGCCA AATCGACATC AACCCGGCGG AGTTTGCCAA GTACAAGCTG 
GATAAGCCCA TCGTTGATAT TGGGGGGATC TCCGGCATGT TATGGATTCA TTTGCTAGCA 
GGTCGCGGCC TAAGAACCGC TCCAGAGGGA GCAGCAGGAA CGGCGACACA GGGCCAAACC 
AGAGATCTTT ACTGCGTAAT CGAGTGCGAT CGAGTACATA AAGCACGGAC TGTGGTACGA 
TCGGGTGACC TGCAGTTCGA CTGGGACGAG TCGTTTGAGC TCGACTTGGT GGGCAACAAA 
CAGTTAGATG TACTAGTCTA CTCATGGGAC CCGCAGCACA GACACAAGCT GTGTTACCGA 
GGCGCAATCT CGTTATCGTC GATCCTCCGT CAGTCTCCAC TTCATCAACT GGCCTTGAAG 
GTTGAACCAA GAGGTACGAT ATACATTCGC ATGCGGCACA CGGATCCACT GGCTCTCTAC 
AAGAGAAGGG GACTTCCGAG CCTGAGAGCA GGTTACCCTA CGCTCTTCGG CGCTGATTTG 
GAAACGGTGG TGAATCGGGA GTCTAAGAAT GCTCCCGGAA GTGCACCTGT TCCCATCGTA 
TTAAGGCGCT GTGTGGAGGA GGTGGAACGG CGTGGTCTTG ATATAATCGG GTTGTACCGC 
CTGTGCGGCT CGGCTACCAA GAAGCGATTG CTACGCGAGG CCTTTGAGCG CAATAGCCGT 
GCAGTGGAAT TGACCCCGGA ACATGTTCCT GACATCAACG TCATCACCGG CGTCCTCAAG 
GATTACCTTA GGGAGCTGCC CGAGCCGCTG TTCACGCGCT GTCTTTTCCA GATGACGGTG 
GATGCCTTAG CTGTCTGCCT GCCAGATGAC CCAGAGGGCA ATGCGAAACT GATGCTTAGC 
ATACTCGACT GCCTACCGCG GGCGAACAGG GCCACCCTGG TATTCCTTCT TGATCACCTC 
TCGCTGGTCG TTTCAAACTC GGAGCGGAAC AAGATGTCAG CCCAGGCGTT GGCCACCGTG 
ATGGGCCCAC CGCTGATGCT GCATTCGGCG AGTGCGCAGC CGGGTGCTGA CATCGATCAC 
GCTCAGCCGA TCGCGGTGCT TAAGTATCTT CTGCAGATCT GGCCGCAGCC GCAGGCGCAG 
CATCAGCAGA TGGCGCAGCA CATGGGCGGC GCTGCGGGGG CAATGATGGG CGGTTTGGTC 
ACCGCCGGGA GCATGAGCAA TATGGCCGGT GTTGCTTCAG GTCGGCGCGG CGAGTCAACA 
GGGCAGCGTG GAAGCAAAGT CAGTGCGTTG CCAGCGGACA GACAGCAACT TCTACTGCAG 
CAGCAGGCGC AGCTCATGGC GGCGGGCAAT CTTCTGCGCT CGTCCACTTC GGTAACCAAC 
ATACTCTCCC AAGGCCATCC TCAGCTCTCA GCCACAGCCA ACAATCATCT GTATCAATCA 
GTAGTGGGTC AGTTAGCTCA ATCGCATCGA GCCTTGCAAC AAGCGGTGCA ACAGCCCTAT 
CAATTGGGGG GCTCAGTGGG CTCAGCAATT CCCGACCCAT CGCCACTGCC ACTTCCAGGC 
ACACCCTCCC CCGGCAGTAG CTCAGCATCG ACGGGCTCAG GCTCCGGATC GGGTAAAAGC 
ACGGACACCA TCAAGCGCGG TGCTTCGCCT GTTTCCGTTA AGCAAGTCAA GATCGTCGAC 
CAGCCATCCA GCCCATACTC CATTGTGATG AAGAAACCGC CGTTGCAAAA AGATGCGCCC 
GTTGAAATCA CCACCCCCAC CACACAGGCT GATACAGAGT CGACCTTGGG ATGCAAGGAA 
AGCAATGGAA CAGCTTCTCG AAGGGGAAAT GTAGACTTTT ATGATACGCA TAAGACGCAG 
GCAAAGAGTG TGGTAAACGA GGAATCCAGC TACAGCTCCA AGTACACTGG CTCGGAGACC 
AAGAAGATCA TCCCTGGCAA CAGTAGCTAC ACTCCCAGCA AGGCGAATGC TAGTGGCCTT 
TCTGGCGGCG AAGACTACAA AGCAATGCGT AACAAGTCGA GCGCTACATC CAGCTCCAGT 
TCATCGCAGG CAACCGTTTT GAGTGCTGGC TCTACCGCCA CATCAGCCCC GACCACTTCT 
TCAGACGATT CGGATGACCT GGTCTCCTAC AAGTCATCGG CGTCCACAAA TGCCTTGCTG 
GCCCAATCGC AGGCCATGAC CACCAGCCAG CTGATGTCCA AGTATCTTAA GCGGGAGCCA 
CGAGTACAGT TTACGCCAAT CAAGTCACCG GAATCTCCCT CGCCACCGGG TAGCGGCGAT 
GGTCTGCCGA AGGGTACTTA CCAACTAGTT ACGCCTATCT CGGGATCGTC GAGCAAACCG 
GGTGCTACAA CTGGAGCTAT AAGCAAGTAT ACCACCGGCT CAGTGGAATC ATCTATTAAT 
GCGAACAGCC AGAAGTTGTC ATCGCCCTCA CGATTGTGCA ACAGCAAGGA TAGCAATAGC 
AGGACTGGAA CGGCCAGTAG TACGACCCCC GCGACCTCCA TGGTTTCTAC AGGTCGGCGT 
TTGTTTGACA GTCTCGCCTC TTCATCGTCC TCGGAAACGG AGACCAAGAC GTACATAGGT 
GGCACCACTG CAGCCAGTGG AGCCATTACC ACCACCATCT ACACCAACGA CACCAAGAAC 
TCCGGCAGCA GTAGCAGTAA GTCGGGAATA GGAGGAGGGT CTGGAACTGG GTTGGGAGCA 
GTTTCGGGAG CAAGCTCCGA GACCCGAAGC TTCGGCAGTA CGCTATTTGG CAGTAGTGGC 
TTAGGTAATG GCAATGGTAG TAGCCACAAC CACTCTAGCG CCAGTCCAAG TCCCTTCACC 
ACCACCAACG GCAACGGTAA CCACAATACC ATGCATCTGT ACGGCACCTT GCCCAAAAAC 
GGAACTTCCA CGGGGGCAGC TCTGTTTGGC GGATCGGCCA ACAGTTCTTC GTACCACTCT 
TCGGCGAGTG GAAGTGGAGC CGGAACCGCC AGCAGTAGTG GGGTCAGCTC TATGACTGGC 
TCCACAAACA GCTACGACTT CTACACGAGC ACAAGTTCCA CTGTGAGCAG TTCGCGCCCC 
TTTGCCAATG GAGGCAACAA CTATCACACA CTGGGAACCT ATAGGGCTCA GTACGCCGCC 
ACTAACCCCT TCCTGGACGC ATTTGATGAA AAGCCGGGCA GCAACGGGGG TAATGCCCAC 
GGGGAGGAGA AGCTAGGCGC GGACAAGGGC CACCACAGAG CGGCCGTTAT GGCCTTTCAG 
TCTTCAGGAG ACTCGAAAAA CGGTAGCGAT GAGTACGATG ACATCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP100F ( NM_001260467.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247396.1 Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant F, mRNA.

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CloneID	OFa07756
Clone ID Related Accession (Same CDS sequence)	NM_001260467.2
Accession Version	NM_001260467.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 100F, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260467.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTGATA GTGCGACCAC GGGATGTTTC CTGACCAGAA GCAGCCATCG CAAGGAAAAT 
GGAAGATCAG TTCCTGATGT CACCGCAAGC CCGGGACGGG CCCCGCCCGG ACCACTGCCC 
GCCAACCAGA TGCCTGCGAT GGGCAACCAG CAACACCATG GAAACCAGCA ACATCATGGA 
AACCAGCAGC AACATCATGG CAATCAGCAC AGCAACCATC GAGGTCAGAG CGGAAGTTTG 
TCAAACGCCG CCGGGGTCAA GGACCCGGTG ATGCTACAGG GCGATTTTCG GAAAGTCAGC 
GGAATCAGCT CAGAGATCTT TCGGCAGATA GAAGCCGTCG AGAATGACCA CGACCCAAAC 
ACGGCGGCGG CTTTGGAGGC GGTGGAGAGA CGCGGTGAGA TGATCGTGCG CGTCCTGGAG 
CCGCGTTGCA TGGGCAGCAA ACAGGCGGTG GACGCTGCCC ACAAGCTGAT GAACAAGGCG 
GACGCACGGC ACACCGTGCA GCTGGTAGAA ATAGTCAAGC GGCCGGGCCA GACGCTGGGC 
TTGTACATCC GCGAGGGAAA TGGGGCCGAT CGAACTGATG GCGTCTTTAT TTCGCGGATT 
GCACTGGAGT CGGCTGTCTA CAACAGCGGC TGCTTGCGGG TGGGCGACGA AATCCTTGCG 
GTAAATCTGG TGGACGTGAC ACACATGTCC TTAGACGATG TCGTCATTAT TATGTCAATT 
CCGCGCCGCT TGGTGCTGGC TATACGTCAG CGGCGGGGCA ATAGAGGCAC AGGATCTCCT 
GGACCGCCAA CGCTCTCTAG GCCAGAACAG AAGCCCCCAC CGGTTGTTGT CATCAAGCGA 
GATTTGCGAG ACGAGGATCT GGACGAGACG GACCGGATGC CGAGGCCGCG CTCATCACGT 
GACAGACGAG ATGGTCGCGA AATGACGGAA TCTCGTTCTA GGCTAGGTCT GGGTCTTAAT 
AATTACAGCC CGCAGTCCGA GCAGTTGGAT ATGTACTACA ATACCCGCGG CGGAGGTGGA 
GGAGCCATGG GTGAGCCTCC AAACTGGGGC TATAAACCAC CACCGCCACC GTCTTCGGTC 
ATTACGGAGC AACCCACGAA AGCACATGCT TTTGCGCCGT CGCATGCTTA CTACCAAAAT 
GCCGGCACTC TTGAAAGCTT GGCGGAGAAG GTACATGCAT TCTATCCTGG ACAGCCTGGT 
GGTCCGCCAG TGGGTCCCTC GAGAAGAATG TCAACGGGAA CTGGAAACGT TGGCCTCGCT 
CAGCAACATG CCAGATTTCC GCGATCTGGC TCAGATCAGC ATTTACCTCG CGTAGAATAT 
TCGGACTATT CCAATTCACT GGGGCGGCAT TCCCTTCTGC GATCAAGTTT AAAACCTGGA 
ACGACCGGTG GAGCTCCAAT GCAGGTAGGA GTGGGAGGCA CTCTTGGCCG ATACGGCCGC 
TATGATCAAC AGAGAGCCGG TGTATCCAAG TACGGCCCTC CATCTGGTGG AGCTCAATCG 
CTGACCCGTC GTTCCAGACC AAATTTGGAC TATTCCAGCG ATACGGAAGC CACAATTGGG 
CCCAGGCCAA GTTACTACTA CTATAACAGA CCTGCCATCG GCAGTATGTC GAGGGGATCA 
GGTGGAGCAG GCGGAGGAGT TGGCGCAGCT TCAACGGCTG CCTTGCTGGC TGGAGCTGCT 
GATCTTAACA AATTTAACTC GTTGCCACGA GAGCGCCCCG GGACGAGACT GCAGGGAATT 
CGTTCCAGAA TGGGAGATCG TTTGGTGGAC GAAAATGACG GAAATACTTC GGCACCCGAG 
TTCGATGTAC GGAGAGGAAG GGACTTACGT CAGCGAATTA CCGCCAGTCC GTCGATATTT 
ACGGCAGATG AATACCGCGC CTGGCTCAGA AGGGCGCCTA GCAGTTCCGC AATTGCGGAA 
CAAATGCGAA TGACGCGGGA CATGTTTGCC CAGCCACGGG CTCAGCGATT TTCGTGTAGC 
GCTGAGAACA TCCATGATGC ACTGAGAAAT ACGGAAAGCA TCTACTCCAG TAGAAACCAT 
ATTCTTGGTA CGGGCACTCT CGATCGGAAC ATGGGTCTTA CCCGACCAAT TTCAGCACTA 
CCCGTGCGCT CCATGTCCTC ACAGCACATT GGAGGAGCGG GTTCCATTCG TTCGCCTAGC 
ATACGGCGCA TGCGACAGTT ACTGGAGCTT TCCGCTGGTC CCGCCAGTCC GAGCGGCAGT 
ATCTTGAGCA CCGGTGGTCA TCAGAGTCCG GCACCAACTC CAAGCGCGAC CCTACCACGC 
CCGCACCGCC AAATCGACAT CAACCCGGCG GAGTTTGCCA AGTACAAGCT GGATAAGCCC 
ATCGTTGATA TTGGGGGGAT CTCCGGCATG TTATGGATTC ATTTGCTAGC AGGTCGCGGC 
CTAAGAACCG CTCCAGAGGG AGCAGCAGGA ACGGCGACAC AGGGCCAAAC CAGAGATCTT 
TACTGCGTAA TCGAGTGCGA TCGAGTACAT AAAGCACGGA CTGTGGTACG ATCGGGTGAC 
CTGCAGTTCG ACTGGGACGA GTCGTTTGAG CTCGACTTGG TGGGCAACAA ACAGTTAGAT 
GTACTAGTCT ACTCATGGGA CCCGCAGCAC AGACACAAGC TGTGTTACCG AGGCGCAATC 
TCGTTATCGT CGATCCTCCG TCAGTCTCCA CTTCATCAAC TGGCCTTGAA GGTTGAACCA 
AGAGGTACGA TATACATTCG CATGCGGCAC ACGGATCCAC TGGCTCTCTA CAAGAGAAGG 
GGACTTCCGA GCCTGAGAGC AGGTTACCCT ACGCTCTTCG GCGCTGATTT GGAAACGGTG 
GTGAATCGGG AGTCTAAGAA TGCTCCCGGA AGTGCACCTG TTCCCATCGT ATTAAGGCGC 
TGTGTGGAGG AGGTGGAACG GCGTGGTCTT GATATAATCG GGTTGTACCG CCTGTGCGGC 
TCGGCTACCA AGAAGCGATT GCTACGCGAG GCCTTTGAGC GCAATAGCCG TGCAGTGGAA 
TTGACCCCGG AACATGTTCC TGACATCAAC GTCATCACCG GCGTCCTCAA GGATTACCTT 
AGGGAGCTGC CCGAGCCGCT GTTCACGCGC TGTCTTTTCC AGATGACGGT GGATGCCTTA 
GCTGTCTGCC TGCCAGATGA CCCAGAGGGC AATGCGAAAC TGATGCTTAG CATACTCGAC 
TGCCTACCGC GGGCGAACAG GGCCACCCTG GTATTCCTTC TTGATCACCT CTCGCTGGTC 
GTTTCAAACT CGGAGCGGAA CAAGATGTCA GCCCAGGCGT TGGCCACCGT GATGGGCCCA 
CCGCTGATGC TGCATTCGGC GAGTGCGCAG CCGGGTGCTG ACATCGATCA CGCTCAGCCG 
ATCGCGGTGC TTAAGTATCT TCTGCAGATC TGGCCGCAGC CGCAGGCGCA GCATCAGCAG 
ATGGCGCAGC ACATGGGCGG CGCTGCGGGG GCAATGATGG GCGGTTTGGT CACCGCCGGG 
AGCATGAGCA ATATGGCCGG TGTTGCTTCA GGTCGGCGCG GCGAGTCAAC AGGGCAGCGT 
GGAAGCAAAG TCAGTGCGTT GCCAGCGGAC AGACAGCAAC TTCTACTGCA GCAGCAGGCG 
CAGCTCATGG CGGCGGGCAA TCTTCTGCGC TCGTCCACTT CGGTAACCAA CATACTCTCC 
CAAGGCCATC CTCAGCTCTC AGCCACAGCC AACAATCATC TGTATCAATC AGTAGTGGGT 
CAGTTAGCTC AATCGCATCG AGCCTTGCAA CAAGCGGTGC AACAGCCCTA TCAATTGGGG 
GGCTCAGTGG GCTCAGCAAT TCCCGACCCA TCGCCACTGC CACTTCCAGG CACACCCTCC 
CCCGGCAGTA GCTCAGCATC GACGGGCTCA GGCTCCGGAT CGGGTAAAAG CACGGACACC 
ATCAAGCGCG GTGCTTCGCC TGTTTCCGTT AAGCAAGTCA AGATCGTCGA CCAGCCATCC 
AGCCCATACT CCATTGTGAT GAAGAAACCG CCGTTGCAAA AAGATGCGCC CGTTGAAATC 
ACCACCCCCA CCACACAGGC TGATACAGAG TCGACCTTGG GATGCAAGGA AAGCAATGGA 
ACAGCTTCTC GAAGGGGAAA TGTAGACTTT TATGATACGC ATAAGACGCA GGCAAAGAGT 
GTGGTAAACG AGGAATCCAG CTACAGCTCC AAGTACACTG GCTCGGAGAC CAAGAAGATC 
ATCCCTGGCA ACAGTAGCTA CACTCCCAGC AAGGCGAATG CTAGTGGCCT TTCTGGCGGC 
GAAGACTACA AAGCAATGCG TAACAAGTCG AGCGCTACAT CCAGCTCCAG TTCATCGCAG 
GCAACCGTTT TGAGTGCTGG CTCTACCGCC ACATCAGCCC CGACCACTTC TTCAGACGAT 
TCGGATGACC TGGTCTCCTA CAAGTCATCG GCGTCCACAA ATGCCTTGCT GGCCCAATCG 
CAGGCCATGA CCACCAGCCA GCTGATGTCC AAGTATCTTA AGCGGGAGCC ACGAGTACAG 
TTTACGCCAA TCAAGTCACC GGAATCTCCC TCGCCACCGG GTAGCGGCGA TGGTCTGCCG 
AAGGGTACTT ACCAACTAGT TACGCCTATC TCGGGATCGT CGAGCAAACC GGGTGCTACA 
ACTGGAGCTA TAAGCAAGTA TACCACCGGC TCAGTGGAAT CATCTATTAA TGCGAACAGC 
CAGAAGTTGT CATCGCCCTC ACGATTGTGC AACAGCAAGG ATAGCAATAG CAGGACTGGA 
ACGGCCAGTA GTACGACCCC CGCGACCTCC ATGGTTTCTA CAGGTCGGCG TTTGTTTGAC 
AGTCTCGCCT CTTCATCGTC CTCGGAAACG GAGACCAAGA CGTACATAGG TGGCACCACT 
GCAGCCAGTG GAGCCATTAC CACCACCATC TACACCAACG ACACCAAGAA CTCCGGCAGC 
AGTAGCAGTA AGTCGGGAAT AGGAGGAGGG TCTGGAACTG GGTTGGGAGC AGTTTCGGGA 
GCAAGCTCCG AGACCCGAAG CTTCGGCAGT ACGCTATTTG GCAGTAGTGG CTTAGGTAAT 
GGCAATGGTA GTAGCCACAA CCACTCTAGC GCCAGTCCAA GTCCCTTCAC CACCACCAAC 
GGCAACGGTA ACCACAATAC CATGCATCTG TACGGCACCT TGCCCAAAAA CGGAACTTCC 
ACGGGGGCAG CTCTGTTTGG CGGATCGGCC AACAGTTCTT CGTACCACTC TTCGGCGAGT 
GGAAGTGGAG CCGGAACCGC CAGCAGTAGT GGGGTCAGCT CTATGACTGG CTCCACAAAC 
AGCTACGACT TCTACACGAG CACAAGTTCC ACTGTGAGCA GTTCGCGCCC CTTTGCCAAT 
GGAGGCAACA ACTATCACAC ACTGGGAACC TATAGGGCTC AGTACGCCGC CACTAACCCC 
TTCCTGGACG CATTTGATGA AAAGCCGGGC AGCAACGGGG GTAATGCCCA CGGGGAGGAG 
AAGCTAGGCG CGGACAAGGG CCACCACAGA GCGGCCGTTA TGGCCTTTCA GTCTTCAGGA 
GACTCGAAAA ACGGTAGCGA TGAGTACGAT GACATCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247396.1
CDS	790..6351
Translation	MCDSATTGCFLTRSSHRKENGRSVPDVTASPGRAPPGPLPANQMPAMGNQQHHGNQQHHGNQQQHHGNQHSNHRGQSGSLSNAAGVKDPVMLQGDFRKVSGISSEIFRQIEAVENDHDPNTAAALEAVERRGEMIVRVLEPRCMGSKQAVDAAHKLMNKADARHTVQLVEIVKRPGQTLGLYIREGNGADRTDGVFISRIALESAVYNSGCLRVGDEILAVNLVDVTHMSLDDVVIIMSIPRRLVLAIRQRRGNRGTGSPGPPTLSRPEQKPPPVVVIKRDLRDEDLDETDRMPRPRSSRDRRDGREMTESRSRLGLGLNNYSPQSEQLDMYYNTRGGGGGAMGEPPNWGYKPPPPPSSVITEQPTKAHAFAPSHAYYQNAGTLESLAEKVHAFYPGQPGGPPVGPSRRMSTGTGNVGLAQQHARFPRSGSDQHLPRVEYSDYSNSLGRHSLLRSSLKPGTTGGAPMQVGVGGTLGRYGRYDQQRAGVSKYGPPSGGAQSLTRRSRPNLDYSSDTEATIGPRPSYYYYNRPAIGSMSRGSGGAGGGVGAASTAALLAGAADLNKFNSLPRERPGTRLQGIRSRMGDRLVDENDGNTSAPEFDVRRGRDLRQRITASPSIFTADEYRAWLRRAPSSSAIAEQMRMTRDMFAQPRAQRFSCSAENIHDALRNTESIYSSRNHILGTGTLDRNMGLTRPISALPVRSMSSQHIGGAGSIRSPSIRRMRQLLELSAGPASPSGSILSTGGHQSPAPTPSATLPRPHRQIDINPAEFAKYKLDKPIVDIGGISGMLWIHLLAGRGLRTAPEGAAGTATQGQTRDLYCVIECDRVHKARTVVRSGDLQFDWDESFELDLVGNKQLDVLVYSWDPQHRHKLCYRGAISLSSILRQSPLHQLALKVEPRGTIYIRMRHTDPLALYKRRGLPSLRAGYPTLFGADLETVVNRESKNAPGSAPVPIVLRRCVEEVERRGLDIIGLYRLCGSATKKRLLREAFERNSRAVELTPEHVPDINVITGVLKDYLRELPEPLFTRCLFQMTVDALAVCLPDDPEGNAKLMLSILDCLPRANRATLVFLLDHLSLVVSNSERNKMSAQALATVMGPPLMLHSASAQPGADIDHAQPIAVLKYLLQIWPQPQAQHQQMAQHMGGAAGAMMGGLVTAGSMSNMAGVASGRRGESTGQRGSKVSALPADRQQLLLQQQAQLMAAGNLLRSSTSVTNILSQGHPQLSATANNHLYQSVVGQLAQSHRALQQAVQQPYQLGGSVGSAIPDPSPLPLPGTPSPGSSSASTGSGSGSGKSTDTIKRGASPVSVKQVKIVDQPSSPYSIVMKKPPLQKDAPVEITTPTTQADTESTLGCKESNGTASRRGNVDFYDTHKTQAKSVVNEESSYSSKYTGSETKKIIPGNSSYTPSKANASGLSGGEDYKAMRNKSSATSSSSSSQATVLSAGSTATSAPTTSSDDSDDLVSYKSSASTNALLAQSQAMTTSQLMSKYLKREPRVQFTPIKSPESPSPPGSGDGLPKGTYQLVTPISGSSSKPGATTGAISKYTTGSVESSINANSQKLSSPSRLCNSKDSNSRTGTASSTTPATSMVSTGRRLFDSLASSSSSETETKTYIGGTTAASGAITTTIYTNDTKNSGSSSSKSGIGGGSGTGLGAVSGASSETRSFGSTLFGSSGLGNGNGSSHNHSSASPSPFTTTNGNGNHNTMHLYGTLPKNGTSTGAALFGGSANSSSYHSSASGSGAGTASSSGVSSMTGSTNSYDFYTSTSSTVSSSRPFANGGNNYHTLGTYRAQYAATNPFLDAFDEKPGSNGGNAHGEEKLGADKGHHRAAVMAFQSSGDSKNGSDEYDDIK

Target ORF information:

RefSeq Version	NM_001260467.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 100F (RhoGAP100F), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260467.2

ORF Insert Sequence:

ATGTGTGATA GTGCGACCAC GGGATGTTTC CTGACCAGAA GCAGCCATCG CAAGGAAAAT 
GGAAGATCAG TTCCTGATGT CACCGCAAGC CCGGGACGGG CCCCGCCCGG ACCACTGCCC 
GCCAACCAGA TGCCTGCGAT GGGCAACCAG CAACACCATG GAAACCAGCA ACATCATGGA 
AACCAGCAGC AACATCATGG CAATCAGCAC AGCAACCATC GAGGTCAGAG CGGAAGTTTG 
TCAAACGCCG CCGGGGTCAA GGACCCGGTG ATGCTACAGG GCGATTTTCG GAAAGTCAGC 
GGAATCAGCT CAGAGATCTT TCGGCAGATA GAAGCCGTCG AGAATGACCA CGACCCAAAC 
ACGGCGGCGG CTTTGGAGGC GGTGGAGAGA CGCGGTGAGA TGATCGTGCG CGTCCTGGAG 
CCGCGTTGCA TGGGCAGCAA ACAGGCGGTG GACGCTGCCC ACAAGCTGAT GAACAAGGCG 
GACGCACGGC ACACCGTGCA GCTGGTAGAA ATAGTCAAGC GGCCGGGCCA GACGCTGGGC 
TTGTACATCC GCGAGGGAAA TGGGGCCGAT CGAACTGATG GCGTCTTTAT TTCGCGGATT 
GCACTGGAGT CGGCTGTCTA CAACAGCGGC TGCTTGCGGG TGGGCGACGA AATCCTTGCG 
GTAAATCTGG TGGACGTGAC ACACATGTCC TTAGACGATG TCGTCATTAT TATGTCAATT 
CCGCGCCGCT TGGTGCTGGC TATACGTCAG CGGCGGGGCA ATAGAGGCAC AGGATCTCCT 
GGACCGCCAA CGCTCTCTAG GCCAGAACAG AAGCCCCCAC CGGTTGTTGT CATCAAGCGA 
GATTTGCGAG ACGAGGATCT GGACGAGACG GACCGGATGC CGAGGCCGCG CTCATCACGT 
GACAGACGAG ATGGTCGCGA AATGACGGAA TCTCGTTCTA GGCTAGGTCT GGGTCTTAAT 
AATTACAGCC CGCAGTCCGA GCAGTTGGAT ATGTACTACA ATACCCGCGG CGGAGGTGGA 
GGAGCCATGG GTGAGCCTCC AAACTGGGGC TATAAACCAC CACCGCCACC GTCTTCGGTC 
ATTACGGAGC AACCCACGAA AGCACATGCT TTTGCGCCGT CGCATGCTTA CTACCAAAAT 
GCCGGCACTC TTGAAAGCTT GGCGGAGAAG GTACATGCAT TCTATCCTGG ACAGCCTGGT 
GGTCCGCCAG TGGGTCCCTC GAGAAGAATG TCAACGGGAA CTGGAAACGT TGGCCTCGCT 
CAGCAACATG CCAGATTTCC GCGATCTGGC TCAGATCAGC ATTTACCTCG CGTAGAATAT 
TCGGACTATT CCAATTCACT GGGGCGGCAT TCCCTTCTGC GATCAAGTTT AAAACCTGGA 
ACGACCGGTG GAGCTCCAAT GCAGGTAGGA GTGGGAGGCA CTCTTGGCCG ATACGGCCGC 
TATGATCAAC AGAGAGCCGG TGTATCCAAG TACGGCCCTC CATCTGGTGG AGCTCAATCG 
CTGACCCGTC GTTCCAGACC AAATTTGGAC TATTCCAGCG ATACGGAAGC CACAATTGGG 
CCCAGGCCAA GTTACTACTA CTATAACAGA CCTGCCATCG GCAGTATGTC GAGGGGATCA 
GGTGGAGCAG GCGGAGGAGT TGGCGCAGCT TCAACGGCTG CCTTGCTGGC TGGAGCTGCT 
GATCTTAACA AATTTAACTC GTTGCCACGA GAGCGCCCCG GGACGAGACT GCAGGGAATT 
CGTTCCAGAA TGGGAGATCG TTTGGTGGAC GAAAATGACG GAAATACTTC GGCACCCGAG 
TTCGATGTAC GGAGAGGAAG GGACTTACGT CAGCGAATTA CCGCCAGTCC GTCGATATTT 
ACGGCAGATG AATACCGCGC CTGGCTCAGA AGGGCGCCTA GCAGTTCCGC AATTGCGGAA 
CAAATGCGAA TGACGCGGGA CATGTTTGCC CAGCCACGGG CTCAGCGATT TTCGTGTAGC 
GCTGAGAACA TCCATGATGC ACTGAGAAAT ACGGAAAGCA TCTACTCCAG TAGAAACCAT 
ATTCTTGGTA CGGGCACTCT CGATCGGAAC ATGGGTCTTA CCCGACCAAT TTCAGCACTA 
CCCGTGCGCT CCATGTCCTC ACAGCACATT GGAGGAGCGG GTTCCATTCG TTCGCCTAGC 
ATACGGCGCA TGCGACAGTT ACTGGAGCTT TCCGCTGGTC CCGCCAGTCC GAGCGGCAGT 
ATCTTGAGCA CCGGTGGTCA TCAGAGTCCG GCACCAACTC CAAGCGCGAC CCTACCACGC 
CCGCACCGCC AAATCGACAT CAACCCGGCG GAGTTTGCCA AGTACAAGCT GGATAAGCCC 
ATCGTTGATA TTGGGGGGAT CTCCGGCATG TTATGGATTC ATTTGCTAGC AGGTCGCGGC 
CTAAGAACCG CTCCAGAGGG AGCAGCAGGA ACGGCGACAC AGGGCCAAAC CAGAGATCTT 
TACTGCGTAA TCGAGTGCGA TCGAGTACAT AAAGCACGGA CTGTGGTACG ATCGGGTGAC 
CTGCAGTTCG ACTGGGACGA GTCGTTTGAG CTCGACTTGG TGGGCAACAA ACAGTTAGAT 
GTACTAGTCT ACTCATGGGA CCCGCAGCAC AGACACAAGC TGTGTTACCG AGGCGCAATC 
TCGTTATCGT CGATCCTCCG TCAGTCTCCA CTTCATCAAC TGGCCTTGAA GGTTGAACCA 
AGAGGTACGA TATACATTCG CATGCGGCAC ACGGATCCAC TGGCTCTCTA CAAGAGAAGG 
GGACTTCCGA GCCTGAGAGC AGGTTACCCT ACGCTCTTCG GCGCTGATTT GGAAACGGTG 
GTGAATCGGG AGTCTAAGAA TGCTCCCGGA AGTGCACCTG TTCCCATCGT ATTAAGGCGC 
TGTGTGGAGG AGGTGGAACG GCGTGGTCTT GATATAATCG GGTTGTACCG CCTGTGCGGC 
TCGGCTACCA AGAAGCGATT GCTACGCGAG GCCTTTGAGC GCAATAGCCG TGCAGTGGAA 
TTGACCCCGG AACATGTTCC TGACATCAAC GTCATCACCG GCGTCCTCAA GGATTACCTT 
AGGGAGCTGC CCGAGCCGCT GTTCACGCGC TGTCTTTTCC AGATGACGGT GGATGCCTTA 
GCTGTCTGCC TGCCAGATGA CCCAGAGGGC AATGCGAAAC TGATGCTTAG CATACTCGAC 
TGCCTACCGC GGGCGAACAG GGCCACCCTG GTATTCCTTC TTGATCACCT CTCGCTGGTC 
GTTTCAAACT CGGAGCGGAA CAAGATGTCA GCCCAGGCGT TGGCCACCGT GATGGGCCCA 
CCGCTGATGC TGCATTCGGC GAGTGCGCAG CCGGGTGCTG ACATCGATCA CGCTCAGCCG 
ATCGCGGTGC TTAAGTATCT TCTGCAGATC TGGCCGCAGC CGCAGGCGCA GCATCAGCAG 
ATGGCGCAGC ACATGGGCGG CGCTGCGGGG GCAATGATGG GCGGTTTGGT CACCGCCGGG 
AGCATGAGCA ATATGGCCGG TGTTGCTTCA GGTCGGCGCG GCGAGTCAAC AGGGCAGCGT 
GGAAGCAAAG TCAGTGCGTT GCCAGCGGAC AGACAGCAAC TTCTACTGCA GCAGCAGGCG 
CAGCTCATGG CGGCGGGCAA TCTTCTGCGC TCGTCCACTT CGGTAACCAA CATACTCTCC 
CAAGGCCATC CTCAGCTCTC AGCCACAGCC AACAATCATC TGTATCAATC AGTAGTGGGT 
CAGTTAGCTC AATCGCATCG AGCCTTGCAA CAAGCGGTGC AACAGCCCTA TCAATTGGGG 
GGCTCAGTGG GCTCAGCAAT TCCCGACCCA TCGCCACTGC CACTTCCAGG CACACCCTCC 
CCCGGCAGTA GCTCAGCATC GACGGGCTCA GGCTCCGGAT CGGGTAAAAG CACGGACACC 
ATCAAGCGCG GTGCTTCGCC TGTTTCCGTT AAGCAAGTCA AGATCGTCGA CCAGCCATCC 
AGCCCATACT CCATTGTGAT GAAGAAACCG CCGTTGCAAA AAGATGCGCC CGTTGAAATC 
ACCACCCCCA CCACACAGGC TGATACAGAG TCGACCTTGG GATGCAAGGA AAGCAATGGA 
ACAGCTTCTC GAAGGGGAAA TGTAGACTTT TATGATACGC ATAAGACGCA GGCAAAGAGT 
GTGGTAAACG AGGAATCCAG CTACAGCTCC AAGTACACTG GCTCGGAGAC CAAGAAGATC 
ATCCCTGGCA ACAGTAGCTA CACTCCCAGC AAGGCGAATG CTAGTGGCCT TTCTGGCGGC 
GAAGACTACA AAGCAATGCG TAACAAGTCG AGCGCTACAT CCAGCTCCAG TTCATCGCAG 
GCAACCGTTT TGAGTGCTGG CTCTACCGCC ACATCAGCCC CGACCACTTC TTCAGACGAT 
TCGGATGACC TGGTCTCCTA CAAGTCATCG GCGTCCACAA ATGCCTTGCT GGCCCAATCG 
CAGGCCATGA CCACCAGCCA GCTGATGTCC AAGTATCTTA AGCGGGAGCC ACGAGTACAG 
TTTACGCCAA TCAAGTCACC GGAATCTCCC TCGCCACCGG GTAGCGGCGA TGGTCTGCCG 
AAGGGTACTT ACCAACTAGT TACGCCTATC TCGGGATCGT CGAGCAAACC GGGTGCTACA 
ACTGGAGCTA TAAGCAAGTA TACCACCGGC TCAGTGGAAT CATCTATTAA TGCGAACAGC 
CAGAAGTTGT CATCGCCCTC ACGATTGTGC AACAGCAAGG ATAGCAATAG CAGGACTGGA 
ACGGCCAGTA GTACGACCCC CGCGACCTCC ATGGTTTCTA CAGGTCGGCG TTTGTTTGAC 
AGTCTCGCCT CTTCATCGTC CTCGGAAACG GAGACCAAGA CGTACATAGG TGGCACCACT 
GCAGCCAGTG GAGCCATTAC CACCACCATC TACACCAACG ACACCAAGAA CTCCGGCAGC 
AGTAGCAGTA AGTCGGGAAT AGGAGGAGGG TCTGGAACTG GGTTGGGAGC AGTTTCGGGA 
GCAAGCTCCG AGACCCGAAG CTTCGGCAGT ACGCTATTTG GCAGTAGTGG CTTAGGTAAT 
GGCAATGGTA GTAGCCACAA CCACTCTAGC GCCAGTCCAA GTCCCTTCAC CACCACCAAC 
GGCAACGGTA ACCACAATAC CATGCATCTG TACGGCACCT TGCCCAAAAA CGGAACTTCC 
ACGGGGGCAG CTCTGTTTGG CGGATCGGCC AACAGTTCTT CGTACCACTC TTCGGCGAGT 
GGAAGTGGAG CCGGAACCGC CAGCAGTAGT GGGGTCAGCT CTATGACTGG CTCCACAAAC 
AGCTACGACT TCTACACGAG CACAAGTTCC ACTGTGAGCA GTTCGCGCCC CTTTGCCAAT 
GGAGGCAACA ACTATCACAC ACTGGGAACC TATAGGGCTC AGTACGCCGC CACTAACCCC 
TTCCTGGACG CATTTGATGA AAAGCCGGGC AGCAACGGGG GTAATGCCCA CGGGGAGGAG 
AAGCTAGGCG CGGACAAGGG CCACCACAGA GCGGCCGTTA TGGCCTTTCA GTCTTCAGGA 
GACTCGAAAA ACGGTAGCGA TGAGTACGAT GACATCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RhoGAP100F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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