zfh1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	zfh1
Entrez Gene ID	43650
Full Name	Zn finger homeodomain 1
Synonyms	CG1322,Dmel\CG1322,ZFH-1,ZFH1,Zfh-1,Zfh1,Zfh1a,l(3)00865,zfh-1,zfl-1,zhf1
General protein information	Preferred Names Zn finger homeodomain 1 Names CG1322 gene product from transcript CG1322-RE CG1322-PA CG1322-PB CG1322-PE Zinc-finger homeodomain protein 1 Zn homeodomain 1 zfh1-PA zfh1-PB zfh1-PE zinc finger homeodomain 1 zinc finger homeodomain protein-1 zinc finger homeodomain-1 zinc-finger homeobox gene zinc-finger homeodomain1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100A4-100A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_170522.4	NP_733401.3	Zn finger homeodomain 1, isoform E
NM_057502.5	NP_476850.1	Zn finger homeodomain 1, isoform B
NM_170523.3	NP_733402.1	Zn finger homeodomain 1, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Zfh1 levels are increased in somatic cells that contact germ cells, and Woc is required for a Stat92E-mediated upregulation of zfh1 transcription.
Title: Without children is required for Stat-mediated zfh1 transcription and for germline stem cell differentiation.

zfh1 is an important regulator of the immune response in Drosophila.
Title: Transcription factor zfh1 downregulates Drosophila Imd pathway.

Study shows a novel function for Zfh1 as an anti-apoptotic molecule and uncovers a cryptic JNK-dependent apoptotic programme in ePG10, which is normally blocked by Zfh1.
Title: Zfh1 promotes survival of a peripheral glia subtype by antagonizing a Jun N-terminal kinase-dependent apoptotic pathway.

Data show that cell fate decisions in the dorsal and lateral Twi- and Tin-positive mesoderm of Drosophila melanogaster depend on the antagonistic action of the transcription factor Lame duck and the zinc finger homeodomain factor Zfh1.
Title: Antagonistic function of Lmd and Zfh1 fine tunes cell fate decisions in the Twi and Tin positive mesoderm of Drosophila melanogaster.

Zfh-1 is a presumptive somatic target of Jak/STAT signaling, demonstrating that it is necessary and sufficient to maintain somatic stem cells.
Title: Zfh-1 controls somatic stem cell self-renewal in the Drosophila testis and nonautonomously influences germline stem cell self-renewal.

The following zfh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the zfh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06697	NM_170523.3 Latest version!	Drosophila melanogaster Zn finger homeodomain 1 (zfh1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06695	NM_170522.4 Latest version!	Drosophila melanogaster Zn finger homeodomain 1 (zfh1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa06696	NM_057502.5 Latest version!	Drosophila melanogaster Zn finger homeodomain 1 (zfh1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

zfh1 ( NM_170523.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733402.1 Drosophila melanogaster Zn finger homeodomain 1 (zfh1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06697
Clone ID Related Accession (Same CDS sequence)	NM_170523.3
Accession Version	NM_170523.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Zn finger homeodomain 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_170523.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGCGG CCGCTTGCCT ACTGTCTTCT TCTACTTCTT CGTTCGAGAA GACCTGCCGC 
ATCTGCCACA AGGCCTTCGC GAACGTCTAC CGCCTGCAGA GGCACATGAT CAGCCACGAC 
GAGAGTGCGC TGCTGCGGAA GTTTAAGTGC AAGGAGTGCG ACAAGGCCTT CAAGTTCAAG 
CACCACCTCA AGGAGCACGT GAGGATCCAT TCCGGCGAGA AGCCCTTCGG ATGCGACAAC 
TGCGGCAAGC GATTCTCGCA CTCGGGCAGC TTCTCCTCCC ACATGACCTC CAAGAAGTGC 
ATCAGCATGG GCCTCAAGCT GAACAACAAT CGCGCTCTGC TGAAGCGACT GGAGAAGAGC 
CCGGGATCCG CATCCTCCGC ATCCCGGCGA TCGCCCTCCG ATCACGGAAA GGGCAAGCTG 
CCGGAGCAGC CGTCGCTACC GGGACTACCA CATCCGATGA GCTACTTCGC CAGCGATGCG 
CAGGTGCAAG GTGGAAGTGC GGCACCCGCG CCCTTCCCGC CATTCCATCC GAACTACATG 
AACGCGGCCC TGCTCGCCTT TCCCCACAAC TTTATGGCGG CTGCAGCTGG CCTGGATCCT 
CGGGTGCATC CCTACAGCAT CCAGAGGCTG CTGCAACTTT CCGCCGCCGG TCAGCAGCAG 
AGGGAGGAGG AGCGTGAGGA GCAGCAAAAG CAGCAGCAGC ATGATGAGGA GGAGACCCCC 
GATGAGCCCA AGCTAGTGAT GGATATCGAG GAGCCAGAGA CCAAGGAGAT GGCACCCACG 
CCAGAGGCCA CCGAAGCAGC CACTCCCATT AAGCGGGAGG AGAGCAGGGA AGCCTCTCCC 
GATCCAGAGA GCTACCGTTC CTCCTCGCAA GCAATCAAAC AAGAGCAGGA GCCCCTGAAC 
GTAGCAGAGG AACGTCAAAC TCCTGTGGAA GAACACGCCC CCGTGGAGCA TGCCGCTGAT 
CTGCGCTGCA GTCGCTGCTC CAAGCAGTTC AATCATCCCA CTGAGCTGGT GCAGCACGAG 
AAGGTGCTTT GTGGCCTGAT CAAGGAGGAG CTGGAGCAGC ACTTCCAACA GCAACAGGCC 
ACGTCCTTTG CCTTGGCCTC CGCCAGCGAA GAGGACGAGG AAGACGAGGA GATGGATGTG 
GAGGAGGAGC CCCGCCAGGA GAGTGGCGAA CGGAAAGTCC GCGTGCGAAC GGCCATCAAC 
GAGGAGCAGC AGCAGCAGTT GAAGCAGCAC TACTCCCTGA ACGCTCGACC CAGTAGGGAT 
GAGTTCCGAA TGATAGCAGC TCGTCTGCAG CTGGATCCCC GAGTGGTTCA AGTGTGGTTC 
CAGAACAACC GCTCCAGGGA GCGCAAAATG CAGAGTTTCC AGAACAATCA GGCGGCAGGC 
GCAGCACCGC CAATGCCCAT TGACTCCCAG GCATCTCTAA CCCGGGAGGA TCAACCGCTG 
GACTTGTCCG TGAAGAGAGA TCCCCTCACG CCGAAGAGTG AGAGCTCGCC TCCGTATATA 
GCTCCACCGT CGGGAGAAGC CCTCAATCCC GAAGCAATCA ATCTCAGCAG GAAGTTCTCC 
ACATCCGCAT CGATGTCGCC GGCCTCGATT TCACCGTCAT CCGCGGCAGC CCTCTACTTT 
GGTGCTGCCC CGCCGCCTTC GCCCCCAAAT AGCCAACTGG ATTCCACTCC TCGCAGTGGC 
CAGGCCTTTC CGGGACTACC TCCCTACATG CTGCCCATGT CGCTGCCCAT GGAGGCGCTG 
TTTAAGATGC GCCCGGGCGG AGACTTCGCT TCCAACCATG CCTTGATGAA CAGTATTAAG 
CTGCCCGATT ACCGGGGCAC CAGCTTGAGT CCCGGTGGAT CGGAGAAGCG TTCGTGGCGG 
GACGACGACT CGCGCATCTC ACACGAGGAC GAGTTCGGCG CCGGCGTTCT GATGCCACCG 
AAACCGCGTA GGGGCAAGGC GGAGACCCAT GGACACGCTG GCGATCCCGA TCTGCCCTAT 
GTGTGCGATC AGTGCGACAA GGCCTTCGCC AAGCAGAGTT CGCTGGCGCG ACACAAATAC 
GAGCATTCCG GTCAACGACC CTACCAGTGC ATAGAGTGTC CGAAGGCCTT CAAGCACAAG 
CACCACCTCA CGGAGCACAA GCGTCTGCAC AGCGGCGAGA AGCCCTTTCA GTGCTCCAAG 
TGCCTGAAGC GCTTCTCGCA CTCGGGCAGC TACAGCCAGC ACATGAACCA CCGGTATTCC 
TATTGCAAGC CCTACAGGGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733402.1
CDS	343..2586
Translation	MSAAACLLSSSTSSFEKTCRICHKAFANVYRLQRHMISHDESALLRKFKCKECDKAFKFKHHLKEHVRIHSGEKPFGCDNCGKRFSHSGSFSSHMTSKKCISMGLKLNNNRALLKRLEKSPGSASSASRRSPSDHGKGKLPEQPSLPGLPHPMSYFASDAQVQGGSAAPAPFPPFHPNYMNAALLAFPHNFMAAAAGLDPRVHPYSIQRLLQLSAAGQQQREEEREEQQKQQQHDEEETPDEPKLVMDIEEPETKEMAPTPEATEAATPIKREESREASPDPESYRSSSQAIKQEQEPLNVAEERQTPVEEHAPVEHAADLRCSRCSKQFNHPTELVQHEKVLCGLIKEELEQHFQQQQATSFALASASEEDEEDEEMDVEEEPRQESGERKVRVRTAINEEQQQQLKQHYSLNARPSRDEFRMIAARLQLDPRVVQVWFQNNRSRERKMQSFQNNQAAGAAPPMPIDSQASLTREDQPLDLSVKRDPLTPKSESSPPYIAPPSGEALNPEAINLSRKFSTSASMSPASISPSSAAALYFGAAPPPSPPNSQLDSTPRSGQAFPGLPPYMLPMSLPMEALFKMRPGGDFASNHALMNSIKLPDYRGTSLSPGGSEKRSWRDDDSRISHEDEFGAGVLMPPKPRRGKAETHGHAGDPDLPYVCDQCDKAFAKQSSLARHKYEHSGQRPYQCIECPKAFKHKHHLTEHKRLHSGEKPFQCSKCLKRFSHSGSYSQHMNHRYSYCKPYRE

Target ORF information:

RefSeq Version	NM_170523.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Zn finger homeodomain 1 (zfh1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170523.3

ORF Insert Sequence:

ATGTCGGCGG CCGCTTGCCT ACTGTCTTCT TCTACTTCTT CGTTCGAGAA GACCTGCCGC 
ATCTGCCACA AGGCCTTCGC GAACGTCTAC CGCCTGCAGA GGCACATGAT CAGCCACGAC 
GAGAGTGCGC TGCTGCGGAA GTTTAAGTGC AAGGAGTGCG ACAAGGCCTT CAAGTTCAAG 
CACCACCTCA AGGAGCACGT GAGGATCCAT TCCGGCGAGA AGCCCTTCGG ATGCGACAAC 
TGCGGCAAGC GATTCTCGCA CTCGGGCAGC TTCTCCTCCC ACATGACCTC CAAGAAGTGC 
ATCAGCATGG GCCTCAAGCT GAACAACAAT CGCGCTCTGC TGAAGCGACT GGAGAAGAGC 
CCGGGATCCG CATCCTCCGC ATCCCGGCGA TCGCCCTCCG ATCACGGAAA GGGCAAGCTG 
CCGGAGCAGC CGTCGCTACC GGGACTACCA CATCCGATGA GCTACTTCGC CAGCGATGCG 
CAGGTGCAAG GTGGAAGTGC GGCACCCGCG CCCTTCCCGC CATTCCATCC GAACTACATG 
AACGCGGCCC TGCTCGCCTT TCCCCACAAC TTTATGGCGG CTGCAGCTGG CCTGGATCCT 
CGGGTGCATC CCTACAGCAT CCAGAGGCTG CTGCAACTTT CCGCCGCCGG TCAGCAGCAG 
AGGGAGGAGG AGCGTGAGGA GCAGCAAAAG CAGCAGCAGC ATGATGAGGA GGAGACCCCC 
GATGAGCCCA AGCTAGTGAT GGATATCGAG GAGCCAGAGA CCAAGGAGAT GGCACCCACG 
CCAGAGGCCA CCGAAGCAGC CACTCCCATT AAGCGGGAGG AGAGCAGGGA AGCCTCTCCC 
GATCCAGAGA GCTACCGTTC CTCCTCGCAA GCAATCAAAC AAGAGCAGGA GCCCCTGAAC 
GTAGCAGAGG AACGTCAAAC TCCTGTGGAA GAACACGCCC CCGTGGAGCA TGCCGCTGAT 
CTGCGCTGCA GTCGCTGCTC CAAGCAGTTC AATCATCCCA CTGAGCTGGT GCAGCACGAG 
AAGGTGCTTT GTGGCCTGAT CAAGGAGGAG CTGGAGCAGC ACTTCCAACA GCAACAGGCC 
ACGTCCTTTG CCTTGGCCTC CGCCAGCGAA GAGGACGAGG AAGACGAGGA GATGGATGTG 
GAGGAGGAGC CCCGCCAGGA GAGTGGCGAA CGGAAAGTCC GCGTGCGAAC GGCCATCAAC 
GAGGAGCAGC AGCAGCAGTT GAAGCAGCAC TACTCCCTGA ACGCTCGACC CAGTAGGGAT 
GAGTTCCGAA TGATAGCAGC TCGTCTGCAG CTGGATCCCC GAGTGGTTCA AGTGTGGTTC 
CAGAACAACC GCTCCAGGGA GCGCAAAATG CAGAGTTTCC AGAACAATCA GGCGGCAGGC 
GCAGCACCGC CAATGCCCAT TGACTCCCAG GCATCTCTAA CCCGGGAGGA TCAACCGCTG 
GACTTGTCCG TGAAGAGAGA TCCCCTCACG CCGAAGAGTG AGAGCTCGCC TCCGTATATA 
GCTCCACCGT CGGGAGAAGC CCTCAATCCC GAAGCAATCA ATCTCAGCAG GAAGTTCTCC 
ACATCCGCAT CGATGTCGCC GGCCTCGATT TCACCGTCAT CCGCGGCAGC CCTCTACTTT 
GGTGCTGCCC CGCCGCCTTC GCCCCCAAAT AGCCAACTGG ATTCCACTCC TCGCAGTGGC 
CAGGCCTTTC CGGGACTACC TCCCTACATG CTGCCCATGT CGCTGCCCAT GGAGGCGCTG 
TTTAAGATGC GCCCGGGCGG AGACTTCGCT TCCAACCATG CCTTGATGAA CAGTATTAAG 
CTGCCCGATT ACCGGGGCAC CAGCTTGAGT CCCGGTGGAT CGGAGAAGCG TTCGTGGCGG 
GACGACGACT CGCGCATCTC ACACGAGGAC GAGTTCGGCG CCGGCGTTCT GATGCCACCG 
AAACCGCGTA GGGGCAAGGC GGAGACCCAT GGACACGCTG GCGATCCCGA TCTGCCCTAT 
GTGTGCGATC AGTGCGACAA GGCCTTCGCC AAGCAGAGTT CGCTGGCGCG ACACAAATAC 
GAGCATTCCG GTCAACGACC CTACCAGTGC ATAGAGTGTC CGAAGGCCTT CAAGCACAAG 
CACCACCTCA CGGAGCACAA GCGTCTGCAC AGCGGCGAGA AGCCCTTTCA GTGCTCCAAG 
TGCCTGAAGC GCTTCTCGCA CTCGGGCAGC TACAGCCAGC ACATGAACCA CCGGTATTCC 
TATTGCAAGC CCTACAGGGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zfh1 ( NM_170522.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733401.3 Drosophila melanogaster Zn finger homeodomain 1 (zfh1), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa06695
Clone ID Related Accession (Same CDS sequence)	NM_170522.4
Accession Version	NM_170522.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Zn finger homeodomain 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_170522.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGTCCT GTCTGGCGCC GTCGTCTTCA CGTTTCGGCC AAGAGGATAC CATCATTCAG 
CAGAGCATGC CCTCCACCTC CCCCTTCGCC ATGCAGTTTC CCTCGCTGGC CTCCACTTTG 
CTCCACCACA ACCAGTCGCC GAAGCACAGC AATCCGGGAT CCAGTGGGAT CCAGGATGCC 
CATCCCAACC AGCCAGGTGC CGCTGCAGAT GCCTTCCTGG TCAAGTGTAC CCAGTGCCAC 
AAGCGATTCC CCGAGTATCA GTCCCTCAGC GAGCACATTG CCAGCGAGCA TCCCCACGAC 
AAGCTGAACT GCGGAGCTGC CCAGCCGGAG AGCGATGCCG AGGACGAGCA GAGCAACATG 
AGCGGCAGCA GCAGGCGGTA CGCCAAGTCG CCGCTGGCCA GCAACAACAA CAGCAGCACC 
GCCAACGCCA ACAACAACAG CACCAGCAGC CAGTCCATGA ACAACAACAG CGAGCTGGCC 
AAGAACCACA ACAGTGCTAA CAAAATGTCG CCGATGTGCT CACCTGGCTC CCTCACGCCC 
GGCGACCTCT TCGCCCAGCT GCAGCATCCG CCGCCCCAAC TGCCGCCCCA CCTGCACGCC 
CAGTTCATGG CCGCCGCCGC CTCCCTGGCC ATGCAGTCCG CCCGCACCGC CAGCTCGCCC 
AGCCAACAGC AGCAGCAGCA ACTGCAACAG CAGCAGCAAC TGCAGCAGCA GCAGCAGCAT 
CAAATGGCCA TGCAGCAACT GCTGCCGCCG CAACTGCCCG GCAGCAACAG CAGCGTGGGC 
AGCAACTCCG CCTACGATTT GGATCTCAGC GCCCCACGCT CCACATCCAG TCCGGGCTCC 
ACCACCGGCG ACCTGAGTGG CGCCTATCCA TGCATGCAGT GCACCGCCTC CTTCGCCAGC 
CGCGAGCAAC TCGAGCAGCA CGAGCAGCTC CACTCGCCAT GCGGACCGGC GGCGGTCAGC 
AATGTCTCCC AGACCTGCCG CATCTGCCAC AAGGCCTTCG CGAACGTCTA CCGCCTGCAG 
AGGCACATGA TCAGCCACGA CGAGAGTGCG CTGCTGCGGA AGTTTAAGTG CAAGGAGTGC 
GACAAGGCCT TCAAGTTCAA GCACCACCTC AAGGAGCACG TGAGGATCCA TTCCGGCGAG 
AAGCCCTTCG GATGCGACAA CTGCGGCAAG CGATTCTCGC ACTCGGGCAG CTTCTCCTCC 
CACATGACCT CCAAGAAGTG CATCAGCATG GGCCTCAAGC TGAACAACAA TCGCGCTCTG 
CTGAAGCGAC TGGAGAAGAG CCCGGGATCC GCATCCTCCG CATCCCGGCG ATCGCCCTCC 
GATCACGGAA AGGGCAAGCT GCCGGAGCAG CCGTCGCTAC CGGGACTACC ACATCCGATG 
AGCTACTTCG CCAGCGATGC GCAGGTGCAA GGTGGAAGTG CGGCACCCGC GCCCTTCCCG 
CCATTCCATC CGAACTACAT GAACGCGGCC CTGCTCGCCT TTCCCCACAA CTTTATGGCG 
GCTGCAGCTG GCCTGGATCC TCGGGTGCAT CCCTACAGCA TCCAGAGGCT GCTGCAACTT 
TCCGCCGCCG GTCAGCAGCA GAGGGAGGAG GAGCGTGAGG AGCAGCAAAA GCAGCAGCAG 
CATGATGAGG AGGAGACCCC CGATGAGCCC AAGCTAGTGA TGGATATCGA GGAGCCAGAG 
ACCAAGGAGA TGGCACCCAC GCCAGAGGCC ACCGAAGCAG CCACTCCCAT TAAGCGGGAG 
GAGAGCAGGG AAGCCTCTCC CGATCCAGAG AGCTACCGTT CCTCCTCGCA AGCAATCAAA 
CAAGAGCAGG AGCCCCTGAA CGTAGCAGAG GAACGTCAAA CTCCTGTGGA AGAACACGCC 
CCCGTGGAGC ATGCCGCTGA TCTGCGCTGC AGTCGCTGCT CCAAGCAGTT CAATCATCCC 
ACTGAGCTGG TGCAGCACGA GAAGGTGCTT TGTGGCCTGA TCAAGGAGGA GCTGGAGCAG 
CACTTCCAAC AGCAACAGGC CACGTCCTTT GCCTTGGCCT CCGCCAGCGA AGAGGACGAG 
GAAGACGAGG AGATGGATGT GGAGGAGGAG CCCCGCCAGG AGAGTGGCGA ACGGAAAGTC 
CGCGTGCGAA CGGCCATCAA CGAGGAGCAG CAGCAGCAGT TGAAGCAGCA CTACTCCCTG 
AACGCTCGAC CCAGTAGGGA TGAGTTCCGA ATGATAGCAG CTCGTCTGCA GCTGGATCCC 
CGAGTGGTTC AAGTGTGGTT CCAGAACAAC CGCTCCAGGG AGCGCAAAAT GCAGAGTTTC 
CAGAACAATC AGGCGGCAGG CGCAGCACCG CCAATGCCCA TTGACTCCCA GGCATCTCTA 
ACCCGGGAGG ATCAACCGCT GGACTTGTCC GTGAAGAGAG ATCCCCTCAC GCCGAAGAGT 
GAGAGCTCGC CTCCGTATAT AGCTCCACCG TCGGGAGAAG CCCTCAATCC CGAAGCAATC 
AATCTCAGCA GGAAGTTCTC CACATCCGCA TCGATGTCGC CGGCCTCGAT TTCACCGTCA 
TCCGCGGCAG CCCTCTACTT TGGTGCTGCC CCGCCGCCTT CGCCCCCAAA TAGCCAACTG 
GATTCCACTC CTCGCAGTGG CCAGGCCTTT CCGGGACTAC CTCCCTACAT GCTGCCCATG 
TCGCTGCCCA TGGAGGCGCT GTTTAAGATG CGCCCGGGCG GAGACTTCGC TTCCAACCAT 
GCCTTGATGA ACAGTATTAA GCTGCCCGAT TACCGGGGCA CCAGCTTGAG TCCCGGTGGA 
TCGGAGAAGC GTTCGTGGCG GGACGACGAC TCGCGCATCT CACACGAGGA CGAGTTCGGC 
GCCGGCGTTC TGATGCCACC GAAACCGCGT AGGGGCAAGG CGGAGACCCA TGGACACGCT 
GGCGATCCCG ATCTGCCCTA TGTGTGCGAT CAGTGCGACA AGGCCTTCGC CAAGCAGAGT 
TCGCTGGCGC GACACAAATA CGAGCATTCC GGTCAACGAC CCTACCAGTG CATAGAGTGT 
CCGAAGGCCT TCAAGCACAA GCACCACCTC ACGGAGCACA AGCGTCTGCA CAGCGGCGAG 
AAGCCCTTTC AGTGCTCCAA GTGCCTGAAG CGCTTCTCGC ACTCGGGCAG CTACAGCCAG 
CACATGAACC ACCGGTATTC CTATTGCAAG CCCTACAGGG AATAGGTAAG CGACACCTCA 
GTGCCGCCCA CCTGCCAACC AGGAGCCAAT CCCCTAGCGC CGCCAGCGAT GGCCAGCAAT 
CGGGGCCGTC GTCGAGTTCA GGTTCCGGCG GGGAATCCCC CGTACACGCC GCATCATCAG 
CATCCCCACC ATCCGCATCT GGCGGCCATG GCTGCCTTCC AGCAGCAGCA CCAGCAGCAG 
CAGCAACAGC ATCAGCATCA CAGGCTCTCC TGGAATGGAG GTGGAGTGAT GCTGCCGCCG 
CCACCCAGTC AGCAGGCGCA TCGTCCGACG GCTCCACATC CGCCTCCATT GCTTTCTGGC 
TCCGGGTTTG GCCAGCCACC AGCGGCGCAG TTCTTTCACC ACCATGCGGG ACTGCTGCCG 
CCGCCGCCGC CACCGCCACA GACCACGCCC ACGTCCGCGC CCGCCTTCGA CTTCTTCAAT 
GCGAAAATGT TTCAGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733401.3
CDS	359..3979
Translation	MLSCLAPSSSRFGQEDTIIQQSMPSTSPFAMQFPSLASTLLHHNQSPKHSNPGSSGIQDAHPNQPGAAADAFLVKCTQCHKRFPEYQSLSEHIASEHPHDKLNCGAAQPESDAEDEQSNMSGSSRRYAKSPLASNNNSSTANANNNSTSSQSMNNNSELAKNHNSANKMSPMCSPGSLTPGDLFAQLQHPPPQLPPHLHAQFMAAAASLAMQSARTASSPSQQQQQQLQQQQQLQQQQQHQMAMQQLLPPQLPGSNSSVGSNSAYDLDLSAPRSTSSPGSTTGDLSGAYPCMQCTASFASREQLEQHEQLHSPCGPAAVSNVSQTCRICHKAFANVYRLQRHMISHDESALLRKFKCKECDKAFKFKHHLKEHVRIHSGEKPFGCDNCGKRFSHSGSFSSHMTSKKCISMGLKLNNNRALLKRLEKSPGSASSASRRSPSDHGKGKLPEQPSLPGLPHPMSYFASDAQVQGGSAAPAPFPPFHPNYMNAALLAFPHNFMAAAAGLDPRVHPYSIQRLLQLSAAGQQQREEEREEQQKQQQHDEEETPDEPKLVMDIEEPETKEMAPTPEATEAATPIKREESREASPDPESYRSSSQAIKQEQEPLNVAEERQTPVEEHAPVEHAADLRCSRCSKQFNHPTELVQHEKVLCGLIKEELEQHFQQQQATSFALASASEEDEEDEEMDVEEEPRQESGERKVRVRTAINEEQQQQLKQHYSLNARPSRDEFRMIAARLQLDPRVVQVWFQNNRSRERKMQSFQNNQAAGAAPPMPIDSQASLTREDQPLDLSVKRDPLTPKSESSPPYIAPPSGEALNPEAINLSRKFSTSASMSPASISPSSAAALYFGAAPPPSPPNSQLDSTPRSGQAFPGLPPYMLPMSLPMEALFKMRPGGDFASNHALMNSIKLPDYRGTSLSPGGSEKRSWRDDDSRISHEDEFGAGVLMPPKPRRGKAETHGHAGDPDLPYVCDQCDKAFAKQSSLARHKYEHSGQRPYQCIECPKAFKHKHHLTEHKRLHSGEKPFQCSKCLKRFSHSGSYSQHMNHRYSYCKPYRE*VSDTSVPPTCQPGANPLAPPAMASNRGRRRVQVPAGNPPYTPHHQHPHHPHLAAMAAFQQQHQQQQQQHQHHRLSWNGGGVMLPPPPSQQAHRPTAPHPPPLLSGSGFGQPPAAQFFHHHAGLLPPPPPPPQTTPTSAPAFDFFNAKMFQN

Target ORF information:

RefSeq Version	NM_170522.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Zn finger homeodomain 1 (zfh1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170522.4

ORF Insert Sequence:

ATGTTGTCCT GTCTGGCGCC GTCGTCTTCA CGTTTCGGCC AAGAGGATAC CATCATTCAG 
CAGAGCATGC CCTCCACCTC CCCCTTCGCC ATGCAGTTTC CCTCGCTGGC CTCCACTTTG 
CTCCACCACA ACCAGTCGCC GAAGCACAGC AATCCGGGAT CCAGTGGGAT CCAGGATGCC 
CATCCCAACC AGCCAGGTGC CGCTGCAGAT GCCTTCCTGG TCAAGTGTAC CCAGTGCCAC 
AAGCGATTCC CCGAGTATCA GTCCCTCAGC GAGCACATTG CCAGCGAGCA TCCCCACGAC 
AAGCTGAACT GCGGAGCTGC CCAGCCGGAG AGCGATGCCG AGGACGAGCA GAGCAACATG 
AGCGGCAGCA GCAGGCGGTA CGCCAAGTCG CCGCTGGCCA GCAACAACAA CAGCAGCACC 
GCCAACGCCA ACAACAACAG CACCAGCAGC CAGTCCATGA ACAACAACAG CGAGCTGGCC 
AAGAACCACA ACAGTGCTAA CAAAATGTCG CCGATGTGCT CACCTGGCTC CCTCACGCCC 
GGCGACCTCT TCGCCCAGCT GCAGCATCCG CCGCCCCAAC TGCCGCCCCA CCTGCACGCC 
CAGTTCATGG CCGCCGCCGC CTCCCTGGCC ATGCAGTCCG CCCGCACCGC CAGCTCGCCC 
AGCCAACAGC AGCAGCAGCA ACTGCAACAG CAGCAGCAAC TGCAGCAGCA GCAGCAGCAT 
CAAATGGCCA TGCAGCAACT GCTGCCGCCG CAACTGCCCG GCAGCAACAG CAGCGTGGGC 
AGCAACTCCG CCTACGATTT GGATCTCAGC GCCCCACGCT CCACATCCAG TCCGGGCTCC 
ACCACCGGCG ACCTGAGTGG CGCCTATCCA TGCATGCAGT GCACCGCCTC CTTCGCCAGC 
CGCGAGCAAC TCGAGCAGCA CGAGCAGCTC CACTCGCCAT GCGGACCGGC GGCGGTCAGC 
AATGTCTCCC AGACCTGCCG CATCTGCCAC AAGGCCTTCG CGAACGTCTA CCGCCTGCAG 
AGGCACATGA TCAGCCACGA CGAGAGTGCG CTGCTGCGGA AGTTTAAGTG CAAGGAGTGC 
GACAAGGCCT TCAAGTTCAA GCACCACCTC AAGGAGCACG TGAGGATCCA TTCCGGCGAG 
AAGCCCTTCG GATGCGACAA CTGCGGCAAG CGATTCTCGC ACTCGGGCAG CTTCTCCTCC 
CACATGACCT CCAAGAAGTG CATCAGCATG GGCCTCAAGC TGAACAACAA TCGCGCTCTG 
CTGAAGCGAC TGGAGAAGAG CCCGGGATCC GCATCCTCCG CATCCCGGCG ATCGCCCTCC 
GATCACGGAA AGGGCAAGCT GCCGGAGCAG CCGTCGCTAC CGGGACTACC ACATCCGATG 
AGCTACTTCG CCAGCGATGC GCAGGTGCAA GGTGGAAGTG CGGCACCCGC GCCCTTCCCG 
CCATTCCATC CGAACTACAT GAACGCGGCC CTGCTCGCCT TTCCCCACAA CTTTATGGCG 
GCTGCAGCTG GCCTGGATCC TCGGGTGCAT CCCTACAGCA TCCAGAGGCT GCTGCAACTT 
TCCGCCGCCG GTCAGCAGCA GAGGGAGGAG GAGCGTGAGG AGCAGCAAAA GCAGCAGCAG 
CATGATGAGG AGGAGACCCC CGATGAGCCC AAGCTAGTGA TGGATATCGA GGAGCCAGAG 
ACCAAGGAGA TGGCACCCAC GCCAGAGGCC ACCGAAGCAG CCACTCCCAT TAAGCGGGAG 
GAGAGCAGGG AAGCCTCTCC CGATCCAGAG AGCTACCGTT CCTCCTCGCA AGCAATCAAA 
CAAGAGCAGG AGCCCCTGAA CGTAGCAGAG GAACGTCAAA CTCCTGTGGA AGAACACGCC 
CCCGTGGAGC ATGCCGCTGA TCTGCGCTGC AGTCGCTGCT CCAAGCAGTT CAATCATCCC 
ACTGAGCTGG TGCAGCACGA GAAGGTGCTT TGTGGCCTGA TCAAGGAGGA GCTGGAGCAG 
CACTTCCAAC AGCAACAGGC CACGTCCTTT GCCTTGGCCT CCGCCAGCGA AGAGGACGAG 
GAAGACGAGG AGATGGATGT GGAGGAGGAG CCCCGCCAGG AGAGTGGCGA ACGGAAAGTC 
CGCGTGCGAA CGGCCATCAA CGAGGAGCAG CAGCAGCAGT TGAAGCAGCA CTACTCCCTG 
AACGCTCGAC CCAGTAGGGA TGAGTTCCGA ATGATAGCAG CTCGTCTGCA GCTGGATCCC 
CGAGTGGTTC AAGTGTGGTT CCAGAACAAC CGCTCCAGGG AGCGCAAAAT GCAGAGTTTC 
CAGAACAATC AGGCGGCAGG CGCAGCACCG CCAATGCCCA TTGACTCCCA GGCATCTCTA 
ACCCGGGAGG ATCAACCGCT GGACTTGTCC GTGAAGAGAG ATCCCCTCAC GCCGAAGAGT 
GAGAGCTCGC CTCCGTATAT AGCTCCACCG TCGGGAGAAG CCCTCAATCC CGAAGCAATC 
AATCTCAGCA GGAAGTTCTC CACATCCGCA TCGATGTCGC CGGCCTCGAT TTCACCGTCA 
TCCGCGGCAG CCCTCTACTT TGGTGCTGCC CCGCCGCCTT CGCCCCCAAA TAGCCAACTG 
GATTCCACTC CTCGCAGTGG CCAGGCCTTT CCGGGACTAC CTCCCTACAT GCTGCCCATG 
TCGCTGCCCA TGGAGGCGCT GTTTAAGATG CGCCCGGGCG GAGACTTCGC TTCCAACCAT 
GCCTTGATGA ACAGTATTAA GCTGCCCGAT TACCGGGGCA CCAGCTTGAG TCCCGGTGGA 
TCGGAGAAGC GTTCGTGGCG GGACGACGAC TCGCGCATCT CACACGAGGA CGAGTTCGGC 
GCCGGCGTTC TGATGCCACC GAAACCGCGT AGGGGCAAGG CGGAGACCCA TGGACACGCT 
GGCGATCCCG ATCTGCCCTA TGTGTGCGAT CAGTGCGACA AGGCCTTCGC CAAGCAGAGT 
TCGCTGGCGC GACACAAATA CGAGCATTCC GGTCAACGAC CCTACCAGTG CATAGAGTGT 
CCGAAGGCCT TCAAGCACAA GCACCACCTC ACGGAGCACA AGCGTCTGCA CAGCGGCGAG 
AAGCCCTTTC AGTGCTCCAA GTGCCTGAAG CGCTTCTCGC ACTCGGGCAG CTACAGCCAG 
CACATGAACC ACCGGTATTC CTATTGCAAG CCCTACAGGG AATAGGTAAG CGACACCTCA 
GTGCCGCCCA CCTGCCAACC AGGAGCCAAT CCCCTAGCGC CGCCAGCGAT GGCCAGCAAT 
CGGGGCCGTC GTCGAGTTCA GGTTCCGGCG GGGAATCCCC CGTACACGCC GCATCATCAG 
CATCCCCACC ATCCGCATCT GGCGGCCATG GCTGCCTTCC AGCAGCAGCA CCAGCAGCAG 
CAGCAACAGC ATCAGCATCA CAGGCTCTCC TGGAATGGAG GTGGAGTGAT GCTGCCGCCG 
CCACCCAGTC AGCAGGCGCA TCGTCCGACG GCTCCACATC CGCCTCCATT GCTTTCTGGC 
TCCGGGTTTG GCCAGCCACC AGCGGCGCAG TTCTTTCACC ACCATGCGGG ACTGCTGCCG 
CCGCCGCCGC CACCGCCACA GACCACGCCC ACGTCCGCGC CCGCCTTCGA CTTCTTCAAT 
GCGAAAATGT TTCAGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zfh1 ( NM_057502.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476850.1 Drosophila melanogaster Zn finger homeodomain 1 (zfh1), transcript variant B, mRNA.

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CloneID	OFa06696
Clone ID Related Accession (Same CDS sequence)	NM_057502.5
Accession Version	NM_057502.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Zn finger homeodomain 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057502.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGTCCT GTCTGGCGCC GTCGTCTTCA CGTTTCGGCC AAGAGGATAC CATCATTCAG 
CAGAGCATGC CCTCCACCTC CCCCTTCGCC ATGCAGTTTC CCTCGCTGGC CTCCACTTTG 
CTCCACCACA ACCAGTCGCC GAAGCACAGC AATCCGGGAT CCAGTGGGAT CCAGGATGCC 
CATCCCAACC AGCCAGGTGC CGCTGCAGAT GCCTTCCTGG TCAAGTGTAC CCAGTGCCAC 
AAGCGATTCC CCGAGTATCA GTCCCTCAGC GAGCACATTG CCAGCGAGCA TCCCCACGAC 
AAGCTGAACT GCGGAGCTGC CCAGCCGGAG AGCGATGCCG AGGACGAGCA GAGCAACATG 
AGCGGCAGCA GCAGGCGGTA CGCCAAGTCG CCGCTGGCCA GCAACAACAA CAGCAGCACC 
GCCAACGCCA ACAACAACAG CACCAGCAGC CAGTCCATGA ACAACAACAG CGAGCTGGCC 
AAGAACCACA ACAGTGCTAA CAAAATGTCG CCGATGTGCT CACCTGGCTC CCTCACGCCC 
GGCGACCTCT TCGCCCAGCT GCAGCATCCG CCGCCCCAAC TGCCGCCCCA CCTGCACGCC 
CAGTTCATGG CCGCCGCCGC CTCCCTGGCC ATGCAGTCCG CCCGCACCGC CAGCTCGCCC 
AGCCAACAGC AGCAGCAGCA ACTGCAACAG CAGCAGCAAC TGCAGCAGCA GCAGCAGCAT 
CAAATGGCCA TGCAGCAACT GCTGCCGCCG CAACTGCCCG GCAGCAACAG CAGCGTGGGC 
AGCAACTCCG CCTACGATTT GGATCTCAGC GCCCCACGCT CCACATCCAG TCCGGGCTCC 
ACCACCGGCG ACCTGAGTGG CGCCTATCCA TGCATGCAGT GCACCGCCTC CTTCGCCAGC 
CGCGAGCAAC TCGAGCAGCA CGAGCAGCTC CACTCGCCAT GCGGACCGGC GGCGGTCAGC 
AATGTCTCCC AGACCTGCCG CATCTGCCAC AAGGCCTTCG CGAACGTCTA CCGCCTGCAG 
AGGCACATGA TCAGCCACGA CGAGAGTGCG CTGCTGCGGA AGTTTAAGTG CAAGGAGTGC 
GACAAGGCCT TCAAGTTCAA GCACCACCTC AAGGAGCACG TGAGGATCCA TTCCGGCGAG 
AAGCCCTTCG GATGCGACAA CTGCGGCAAG CGATTCTCGC ACTCGGGCAG CTTCTCCTCC 
CACATGACCT CCAAGAAGTG CATCAGCATG GGCCTCAAGC TGAACAACAA TCGCGCTCTG 
CTGAAGCGAC TGGAGAAGAG CCCGGGATCC GCATCCTCCG CATCCCGGCG ATCGCCCTCC 
GATCACGGAA AGGGCAAGCT GCCGGAGCAG CCGTCGCTAC CGGGACTACC ACATCCGATG 
AGCTACTTCG CCAGCGATGC GCAGGTGCAA GGTGGAAGTG CGGCACCCGC GCCCTTCCCG 
CCATTCCATC CGAACTACAT GAACGCGGCC CTGCTCGCCT TTCCCCACAA CTTTATGGCG 
GCTGCAGCTG GCCTGGATCC TCGGGTGCAT CCCTACAGCA TCCAGAGGCT GCTGCAACTT 
TCCGCCGCCG GTCAGCAGCA GAGGGAGGAG GAGCGTGAGG AGCAGCAAAA GCAGCAGCAG 
CATGATGAGG AGGAGACCCC CGATGAGCCC AAGCTAGTGA TGGATATCGA GGAGCCAGAG 
ACCAAGGAGA TGGCACCCAC GCCAGAGGCC ACCGAAGCAG CCACTCCCAT TAAGCGGGAG 
GAGAGCAGGG AAGCCTCTCC CGATCCAGAG AGCTACCGTT CCTCCTCGCA AGCAATCAAA 
CAAGAGCAGG AGCCCCTGAA CGTAGCAGAG GAACGTCAAA CTCCTGTGGA AGAACACGCC 
CCCGTGGAGC ATGCCGCTGA TCTGCGCTGC AGTCGCTGCT CCAAGCAGTT CAATCATCCC 
ACTGAGCTGG TGCAGCACGA GAAGGTGCTT TGTGGCCTGA TCAAGGAGGA GCTGGAGCAG 
CACTTCCAAC AGCAACAGGC CACGTCCTTT GCCTTGGCCT CCGCCAGCGA AGAGGACGAG 
GAAGACGAGG AGATGGATGT GGAGGAGGAG CCCCGCCAGG AGAGTGGCGA ACGGAAAGTC 
CGCGTGCGAA CGGCCATCAA CGAGGAGCAG CAGCAGCAGT TGAAGCAGCA CTACTCCCTG 
AACGCTCGAC CCAGTAGGGA TGAGTTCCGA ATGATAGCAG CTCGTCTGCA GCTGGATCCC 
CGAGTGGTTC AAGTGTGGTT CCAGAACAAC CGCTCCAGGG AGCGCAAAAT GCAGAGTTTC 
CAGAACAATC AGGCGGCAGG CGCAGCACCG CCAATGCCCA TTGACTCCCA GGCATCTCTA 
ACCCGGGAGG ATCAACCGCT GGACTTGTCC GTGAAGAGAG ATCCCCTCAC GCCGAAGAGT 
GAGAGCTCGC CTCCGTATAT AGCTCCACCG TCGGGAGAAG CCCTCAATCC CGAAGCAATC 
AATCTCAGCA GGAAGTTCTC CACATCCGCA TCGATGTCGC CGGCCTCGAT TTCACCGTCA 
TCCGCGGCAG CCCTCTACTT TGGTGCTGCC CCGCCGCCTT CGCCCCCAAA TAGCCAACTG 
GATTCCACTC CTCGCAGTGG CCAGGCCTTT CCGGGACTAC CTCCCTACAT GCTGCCCATG 
TCGCTGCCCA TGGAGGCGCT GTTTAAGATG CGCCCGGGCG GAGACTTCGC TTCCAACCAT 
GCCTTGATGA ACAGTATTAA GCTGCCCGAT TACCGGGGCA CCAGCTTGAG TCCCGGTGGA 
TCGGAGAAGC GTTCGTGGCG GGACGACGAC TCGCGCATCT CACACGAGGA CGAGTTCGGC 
GCCGGCGTTC TGATGCCACC GAAACCGCGT AGGGGCAAGG CGGAGACCCA TGGACACGCT 
GGCGATCCCG ATCTGCCCTA TGTGTGCGAT CAGTGCGACA AGGCCTTCGC CAAGCAGAGT 
TCGCTGGCGC GACACAAATA CGAGCATTCC GGTCAACGAC CCTACCAGTG CATAGAGTGT 
CCGAAGGCCT TCAAGCACAA GCACCACCTC ACGGAGCACA AGCGTCTGCA CAGCGGCGAG 
AAGCCCTTTC AGTGCTCCAA GTGCCTGAAG CGCTTCTCGC ACTCGGGCAG CTACAGCCAG 
CACATGAACC ACCGGTATTC CTATTGCAAG CCCTACAGGG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476850.1
CDS	359..3523
Translation	MLSCLAPSSSRFGQEDTIIQQSMPSTSPFAMQFPSLASTLLHHNQSPKHSNPGSSGIQDAHPNQPGAAADAFLVKCTQCHKRFPEYQSLSEHIASEHPHDKLNCGAAQPESDAEDEQSNMSGSSRRYAKSPLASNNNSSTANANNNSTSSQSMNNNSELAKNHNSANKMSPMCSPGSLTPGDLFAQLQHPPPQLPPHLHAQFMAAAASLAMQSARTASSPSQQQQQQLQQQQQLQQQQQHQMAMQQLLPPQLPGSNSSVGSNSAYDLDLSAPRSTSSPGSTTGDLSGAYPCMQCTASFASREQLEQHEQLHSPCGPAAVSNVSQTCRICHKAFANVYRLQRHMISHDESALLRKFKCKECDKAFKFKHHLKEHVRIHSGEKPFGCDNCGKRFSHSGSFSSHMTSKKCISMGLKLNNNRALLKRLEKSPGSASSASRRSPSDHGKGKLPEQPSLPGLPHPMSYFASDAQVQGGSAAPAPFPPFHPNYMNAALLAFPHNFMAAAAGLDPRVHPYSIQRLLQLSAAGQQQREEEREEQQKQQQHDEEETPDEPKLVMDIEEPETKEMAPTPEATEAATPIKREESREASPDPESYRSSSQAIKQEQEPLNVAEERQTPVEEHAPVEHAADLRCSRCSKQFNHPTELVQHEKVLCGLIKEELEQHFQQQQATSFALASASEEDEEDEEMDVEEEPRQESGERKVRVRTAINEEQQQQLKQHYSLNARPSRDEFRMIAARLQLDPRVVQVWFQNNRSRERKMQSFQNNQAAGAAPPMPIDSQASLTREDQPLDLSVKRDPLTPKSESSPPYIAPPSGEALNPEAINLSRKFSTSASMSPASISPSSAAALYFGAAPPPSPPNSQLDSTPRSGQAFPGLPPYMLPMSLPMEALFKMRPGGDFASNHALMNSIKLPDYRGTSLSPGGSEKRSWRDDDSRISHEDEFGAGVLMPPKPRRGKAETHGHAGDPDLPYVCDQCDKAFAKQSSLARHKYEHSGQRPYQCIECPKAFKHKHHLTEHKRLHSGEKPFQCSKCLKRFSHSGSYSQHMNHRYSYCKPYRE

Target ORF information:

RefSeq Version	NM_057502.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Zn finger homeodomain 1 (zfh1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057502.5

ORF Insert Sequence:

ATGTTGTCCT GTCTGGCGCC GTCGTCTTCA CGTTTCGGCC AAGAGGATAC CATCATTCAG 
CAGAGCATGC CCTCCACCTC CCCCTTCGCC ATGCAGTTTC CCTCGCTGGC CTCCACTTTG 
CTCCACCACA ACCAGTCGCC GAAGCACAGC AATCCGGGAT CCAGTGGGAT CCAGGATGCC 
CATCCCAACC AGCCAGGTGC CGCTGCAGAT GCCTTCCTGG TCAAGTGTAC CCAGTGCCAC 
AAGCGATTCC CCGAGTATCA GTCCCTCAGC GAGCACATTG CCAGCGAGCA TCCCCACGAC 
AAGCTGAACT GCGGAGCTGC CCAGCCGGAG AGCGATGCCG AGGACGAGCA GAGCAACATG 
AGCGGCAGCA GCAGGCGGTA CGCCAAGTCG CCGCTGGCCA GCAACAACAA CAGCAGCACC 
GCCAACGCCA ACAACAACAG CACCAGCAGC CAGTCCATGA ACAACAACAG CGAGCTGGCC 
AAGAACCACA ACAGTGCTAA CAAAATGTCG CCGATGTGCT CACCTGGCTC CCTCACGCCC 
GGCGACCTCT TCGCCCAGCT GCAGCATCCG CCGCCCCAAC TGCCGCCCCA CCTGCACGCC 
CAGTTCATGG CCGCCGCCGC CTCCCTGGCC ATGCAGTCCG CCCGCACCGC CAGCTCGCCC 
AGCCAACAGC AGCAGCAGCA ACTGCAACAG CAGCAGCAAC TGCAGCAGCA GCAGCAGCAT 
CAAATGGCCA TGCAGCAACT GCTGCCGCCG CAACTGCCCG GCAGCAACAG CAGCGTGGGC 
AGCAACTCCG CCTACGATTT GGATCTCAGC GCCCCACGCT CCACATCCAG TCCGGGCTCC 
ACCACCGGCG ACCTGAGTGG CGCCTATCCA TGCATGCAGT GCACCGCCTC CTTCGCCAGC 
CGCGAGCAAC TCGAGCAGCA CGAGCAGCTC CACTCGCCAT GCGGACCGGC GGCGGTCAGC 
AATGTCTCCC AGACCTGCCG CATCTGCCAC AAGGCCTTCG CGAACGTCTA CCGCCTGCAG 
AGGCACATGA TCAGCCACGA CGAGAGTGCG CTGCTGCGGA AGTTTAAGTG CAAGGAGTGC 
GACAAGGCCT TCAAGTTCAA GCACCACCTC AAGGAGCACG TGAGGATCCA TTCCGGCGAG 
AAGCCCTTCG GATGCGACAA CTGCGGCAAG CGATTCTCGC ACTCGGGCAG CTTCTCCTCC 
CACATGACCT CCAAGAAGTG CATCAGCATG GGCCTCAAGC TGAACAACAA TCGCGCTCTG 
CTGAAGCGAC TGGAGAAGAG CCCGGGATCC GCATCCTCCG CATCCCGGCG ATCGCCCTCC 
GATCACGGAA AGGGCAAGCT GCCGGAGCAG CCGTCGCTAC CGGGACTACC ACATCCGATG 
AGCTACTTCG CCAGCGATGC GCAGGTGCAA GGTGGAAGTG CGGCACCCGC GCCCTTCCCG 
CCATTCCATC CGAACTACAT GAACGCGGCC CTGCTCGCCT TTCCCCACAA CTTTATGGCG 
GCTGCAGCTG GCCTGGATCC TCGGGTGCAT CCCTACAGCA TCCAGAGGCT GCTGCAACTT 
TCCGCCGCCG GTCAGCAGCA GAGGGAGGAG GAGCGTGAGG AGCAGCAAAA GCAGCAGCAG 
CATGATGAGG AGGAGACCCC CGATGAGCCC AAGCTAGTGA TGGATATCGA GGAGCCAGAG 
ACCAAGGAGA TGGCACCCAC GCCAGAGGCC ACCGAAGCAG CCACTCCCAT TAAGCGGGAG 
GAGAGCAGGG AAGCCTCTCC CGATCCAGAG AGCTACCGTT CCTCCTCGCA AGCAATCAAA 
CAAGAGCAGG AGCCCCTGAA CGTAGCAGAG GAACGTCAAA CTCCTGTGGA AGAACACGCC 
CCCGTGGAGC ATGCCGCTGA TCTGCGCTGC AGTCGCTGCT CCAAGCAGTT CAATCATCCC 
ACTGAGCTGG TGCAGCACGA GAAGGTGCTT TGTGGCCTGA TCAAGGAGGA GCTGGAGCAG 
CACTTCCAAC AGCAACAGGC CACGTCCTTT GCCTTGGCCT CCGCCAGCGA AGAGGACGAG 
GAAGACGAGG AGATGGATGT GGAGGAGGAG CCCCGCCAGG AGAGTGGCGA ACGGAAAGTC 
CGCGTGCGAA CGGCCATCAA CGAGGAGCAG CAGCAGCAGT TGAAGCAGCA CTACTCCCTG 
AACGCTCGAC CCAGTAGGGA TGAGTTCCGA ATGATAGCAG CTCGTCTGCA GCTGGATCCC 
CGAGTGGTTC AAGTGTGGTT CCAGAACAAC CGCTCCAGGG AGCGCAAAAT GCAGAGTTTC 
CAGAACAATC AGGCGGCAGG CGCAGCACCG CCAATGCCCA TTGACTCCCA GGCATCTCTA 
ACCCGGGAGG ATCAACCGCT GGACTTGTCC GTGAAGAGAG ATCCCCTCAC GCCGAAGAGT 
GAGAGCTCGC CTCCGTATAT AGCTCCACCG TCGGGAGAAG CCCTCAATCC CGAAGCAATC 
AATCTCAGCA GGAAGTTCTC CACATCCGCA TCGATGTCGC CGGCCTCGAT TTCACCGTCA 
TCCGCGGCAG CCCTCTACTT TGGTGCTGCC CCGCCGCCTT CGCCCCCAAA TAGCCAACTG 
GATTCCACTC CTCGCAGTGG CCAGGCCTTT CCGGGACTAC CTCCCTACAT GCTGCCCATG 
TCGCTGCCCA TGGAGGCGCT GTTTAAGATG CGCCCGGGCG GAGACTTCGC TTCCAACCAT 
GCCTTGATGA ACAGTATTAA GCTGCCCGAT TACCGGGGCA CCAGCTTGAG TCCCGGTGGA 
TCGGAGAAGC GTTCGTGGCG GGACGACGAC TCGCGCATCT CACACGAGGA CGAGTTCGGC 
GCCGGCGTTC TGATGCCACC GAAACCGCGT AGGGGCAAGG CGGAGACCCA TGGACACGCT 
GGCGATCCCG ATCTGCCCTA TGTGTGCGAT CAGTGCGACA AGGCCTTCGC CAAGCAGAGT 
TCGCTGGCGC GACACAAATA CGAGCATTCC GGTCAACGAC CCTACCAGTG CATAGAGTGT 
CCGAAGGCCT TCAAGCACAA GCACCACCTC ACGGAGCACA AGCGTCTGCA CAGCGGCGAG 
AAGCCCTTTC AGTGCTCCAA GTGCCTGAAG CGCTTCTCGC ACTCGGGCAG CTACAGCCAG 
CACATGAACC ACCGGTATTC CTATTGCAAG CCCTACAGGG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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zfh1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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