Mlc2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mlc2
Entrez Gene ID	43587
Full Name	Myosin light chain 2
Synonyms	CG2184,DmMLC2,Dmel\CG2184,Dmlc,Dmlc2,Ifm(3)99Eb,LC2,MLC,MLC 2,MLC-2,MLC2,MRLC,MYL2,RLC,RLC1,RLC2,dMLC-2,dro MLC-2,l(3)99Eb,mlc-2,mlc2,myosin
General protein information	Preferred Names Myosin light chain 2 Names CG2184 gene product from transcript CG2184-RA CG2184-PA Mlc2-PA muscle-specific myosin regulatory light chain myosin LC2 myosin alkali light chain 2 myosin light Chain-2 myosin light chain 2 myosin light-chain 2 myosin regulatory light chain
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99E1-99E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_079847.3	NP_524586.1	myosin light chain 2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Mlc2	NP_524586.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001622	XP_321477.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mutations in DMLC2 manifest differently in song and flight
Title: Mutations of the Drosophila myosin regulatory light chain affect courtship song and reduce reproductive success.

The functional and structural role of the regulatory light chain in chemically skinned fibers at various thick and thin filament lattice spacings.
Title: Regulatory light chain phosphorylation and N-terminal extension increase cross-bridge binding and power output in Drosophila at in vivo myofilament lattice spacing.

cycles of myosin-mediated contraction and relaxation, tightly linked to Cdc2 and Rho kinase activity, are directly responsible for the axial expansion of the syncytial nuclei.
Title: Cortical recruitment of nonmuscle myosin II in early syncytial Drosophila embryos: its role in nuclear axial expansion and its regulation by Cdc2 activity.

Changes in myofibrillar structure and function produced by N-terminal deletion of the regulatory light chain in Drosophila.
Title: Changes in myofibrillar structure and function produced by N-terminal deletion of the regulatory light chain in Drosophila.

The following Mlc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mlc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06621	NM_079847.3 Latest version!	Drosophila melanogaster myosin light chain 2 (Mlc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mlc2 ( NM_079847.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524586.1 Drosophila melanogaster myosin light chain 2 (Mlc2), mRNA.

Price & Availability↑

CloneID	OFa06621
Clone ID Related Accession (Same CDS sequence)	NM_079847.3
Accession Version	NM_079847.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin light chain 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Nov 6, 2002 this sequence version replaced NM_079847.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGATG AGAAGAAGAA GGTTAAGAAG AAGAAGACCA AGGAAGAGGG TGGTACTTCC 
GAAACCGCTT CTGAGGCCGC ATCCGAGGCA GCAACCCCGG CACCAGCTGC CACTCCTGCC 
CCGGCCGCAT CTGCCACTGG TTCGAAGAGA GCGTCGGGCG GATCCCGTGG CTCCAGGAAG 
TCGAAGCGCG CTGGCTCCTC GGTCTTCTCT GTGTTCTCCC AGAAGCAGAT CGCCGAGTTC 
AAGGAGGCCT TCCAACTCAT GGATGCCGAC AAGGACGGTA TTATTGGCAA GAACGATCTG 
CGCGCTGCCT TCGACTCCGT CGGCAAGATC GCCAACGACA AGGAGTTGGA CGCCATGCTG 
GGCGAGGCCT CGGGTCCGAT CAACTTCACC CAGTTGCTGA CCCTGTTCGC CAACCGCATG 
GCCACCTCCG GTGCCAACGA TGAAGACGAA GTTGTTATTG CTGCCTTCAA AACATTCGAT 
AACGATGGTC TCATCGACGG TGACAAATTC CGCGAAATGC TCATGAACTT CGGTGACAAG 
TTCACCATGA AGGAGGTTGA TGATGCCTAC GATCAGATGG TGATCGACGA CAAGAACCAG 
ATCGATACCG CCGCCCTGAT CGAGATGCTC ACCGGCAAGG GTGAAGAGGA GGAGGAGGAG 
GCCGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524586.1
CDS	66..734
Translation	MADEKKKVKKKKTKEEGGTSETASEAASEAATPAPAATPAPAASATGSKRASGGSRGSRKSKRAGSSVFSVFSQKQIAEFKEAFQLMDADKDGIIGKNDLRAAFDSVGKIANDKELDAMLGEASGPINFTQLLTLFANRMATSGANDEDEVVIAAFKTFDNDGLIDGDKFREMLMNFGDKFTMKEVDDAYDQMVIDDKNQIDTAALIEMLTGKGEEEEEEAA

Target ORF information:

RefSeq Version	NM_079847.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin light chain 2 (Mlc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079847.3

ORF Insert Sequence:

ATGGCCGATG AGAAGAAGAA GGTTAAGAAG AAGAAGACCA AGGAAGAGGG TGGTACTTCC 
GAAACCGCTT CTGAGGCCGC ATCCGAGGCA GCAACCCCGG CACCAGCTGC CACTCCTGCC 
CCGGCCGCAT CTGCCACTGG TTCGAAGAGA GCGTCGGGCG GATCCCGTGG CTCCAGGAAG 
TCGAAGCGCG CTGGCTCCTC GGTCTTCTCT GTGTTCTCCC AGAAGCAGAT CGCCGAGTTC 
AAGGAGGCCT TCCAACTCAT GGATGCCGAC AAGGACGGTA TTATTGGCAA GAACGATCTG 
CGCGCTGCCT TCGACTCCGT CGGCAAGATC GCCAACGACA AGGAGTTGGA CGCCATGCTG 
GGCGAGGCCT CGGGTCCGAT CAACTTCACC CAGTTGCTGA CCCTGTTCGC CAACCGCATG 
GCCACCTCCG GTGCCAACGA TGAAGACGAA GTTGTTATTG CTGCCTTCAA AACATTCGAT 
AACGATGGTC TCATCGACGG TGACAAATTC CGCGAAATGC TCATGAACTT CGGTGACAAG 
TTCACCATGA AGGAGGTTGA TGATGCCTAC GATCAGATGG TGATCGACGA CAAGAACCAG 
ATCGATACCG CCGCCCTGAT CGAGATGCTC ACCGGCAAGG GTGAAGAGGA GGAGGAGGAG 
GCCGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mlc2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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