Nep7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nep7
Entrez Gene ID	43356
Full Name	CG5527 gene product from transcript CG5527-RA
Synonyms	Dmel\CG5527
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98B6-98B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_143346.3	NP_651603.1	neprilysin 7

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG8358	NP_649924.1
Drosophila melanogaster (fruit fly)	CG5527	NP_651603.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nep7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nep7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06379	NM_143346.3 Latest version!	Drosophila melanogaster neprilysin 7 (Nep7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nep7 ( NM_143346.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651603.1 Drosophila melanogaster neprilysin 7 (Nep7), mRNA.

Price & Availability↑

CloneID	OFa06379
Clone ID Related Accession (Same CDS sequence)	NM_143346.3
Accession Version	NM_143346.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neprilysin 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_143346.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCTCAA TCTCGAGCTA CTTTCGACTG CCCCTATACA CGCGGCTGGC AGTTTGCATC 
CTGATCTGCG TGATTTTGAA TGACTGCGAG GCCAGGAGTA TTGGCGACGA GTCCCATGAG 
AACGCGATTA CGGATGAGAC GGCCGACTAC CTGCGATCCC ATGGCGCCAT GATGAAGTCC 
TATATGAACT TCAGCGTCGA TCCGTGCGAC GACTTCTACG AGTTCGCGTG CGGCAACTGG 
AAGAACGTGA AAACGCCGCG GCAATCGCCC CATAGGCGGA GCAATATTCT GGACATCAGC 
TACACACTGA TGGACGTGAC GGAGCAGCTG CTGACCAGGA CAAAGTTGGC GGAGGCACTG 
AACGTGTCCG CCGAGCTGCT CGTTGCACAA CGCTTCTACA ATTCCTGCCT GGCGGCCGAG 
CTCTTTCCGC GGCCCGCTGC CGATCCCGCT TATCTATCGG TTATCAGGTC GCTGGGTGGT 
TTTCCCGCCG TCGACAGAGA CGCCTGGAAC GCCTCCAGCT TCAGTTGGTT CAACATGAGC 
GCCCATCTCA GCAACTACGG CGTTCGCAGT TTGATCCACG AAGAAATTCT GCCGCAGCAT 
CCCTTCAATC CGTACTTCAA GCTACCCGAA CTGGGCTTTG ACCATGTCGT CCACACCGCT 
CACGTTGCCA ATACGAGCTC GCGGGCCTAC CAGCTCAACG AGCATCGCAT GCGTGGCTAC 
CTGAAGGAAT ACAATCTCAC GAAGGATGAA ATCTCTGCCG TGATCGAAGG CGTCTTTGCT 
TTTTGGAGCG AGGCGCTCAG TATTGAGGAT AAGTTCGAGA GCGATAGGGA TAAGTGCGAT 
TTGCTCAGTG AGATTGAGGA GGTGCCGCGA TTTGGTCAGT GGAGGAGCTA CTACGAGATA 
GCCTGGAATG GTATAGACTT TTACAACGGC TCGTCCGGTG GCGAATTCTG CGACTTTTAC 
TATGTGGAGC TCGACAAGGT GTGCGCCAAG CATACGGAGG CGGTGGCCAA TTATCTAGCC 
ATGCGGTTTC TCTACCGCAT GGACGACAAG CTGAAGGAGC CCAAGCAGCC GGAGGACCAA 
AAGGTTAACT GCTTGATGAT CGTCCAGATG ACGCTGCCAA ATCTGTTCAA CAGGCTCTAC 
ATGGCGGAGC ACTTCACCGA CGAGAAGCAC TCGGAGATCT CTGACATGGT GACCGAAATG 
CGAAAGGCCC AGCGACAGAT CCTCGAGAGT GTGGAGTGGT TGGACGCGGA GACGCGAGCA 
GAGGCTCTGA GCAAGGAGGC CGGCATCACG CCAGTAATCG GCAGTTACAA AAACGAAGAG 
CTGTCGGACA CTCTAATCCG CGAGATCAAC AATCTGACCA TTGTTGAAGG CAGCTATGCC 
CAGAGTTTGA TCAATCTGCG AGTATTGGGC ACCTATCTTC ATCGCTACGC TGGCTTACAT 
GCGGACGAGC TGCCCAAGGA CACAAAGCCA CTGACACTGC TCGTGGGAAT GCAGGTGAAC 
GCCTTCTACT ACAATCTGGA TAACTCGGTG TACGTGATGG CGGGAGTACT GCATCCGCCT 
GCGTACCACC GTTCATGGCC CGATTCCTTG AAGTTCGGCA CCATTGGCTA TCTGATAGGT 
CACGAGTTGA CCCACGGCTT CGATACGGAG GGCTCCAGTT TCGACAGCGC CGGCGAAGTA 
CGCGACTGGT GGTCCAGGAA GTCGGAGGCG GTATTCCAGG AACGGGCGCA GTGCTATGTG 
GATTACTTCA ACCAATACTT GATACCGGAA ATCAACCAGA CAATTCGCGG CAATGAGACA 
AGGGACGAGA ACATAGCGGA CGCCGGTGGC TTGAGGGCCG CACTCACCGG CTATCGAAAC 
CACATGAAGC AGCTGCAACG AAGTGGAGCG GATGGGGAGA CGTTGGCTCA CAAAAACGAG 
CAGATGCCCG GCCTCGACCT GTCGCCGGAG CAGCTCTTCT TCGTGGGATT CGCGCAGCTG 
TGGTGCGCCG ACTACGAGCC GGAGCACTAC TGGGAGGAGC TCACTGGCGC ACATACCGTC 
GATAAGTATC GGGTGCTGGG TGCCGTTTCC AACAACGACG ATTTTGCTGA GGTCTACAAG 
TGCCCACTGG GCAGTCCAAT GCATCCGAAG GCTGAGAGTT GTCGGATGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651603.1
CDS	105..2258
Translation	MLSISSYFRLPLYTRLAVCILICVILNDCEARSIGDESHENAITDETADYLRSHGAMMKSYMNFSVDPCDDFYEFACGNWKNVKTPRQSPHRRSNILDISYTLMDVTEQLLTRTKLAEALNVSAELLVAQRFYNSCLAAELFPRPAADPAYLSVIRSLGGFPAVDRDAWNASSFSWFNMSAHLSNYGVRSLIHEEILPQHPFNPYFKLPELGFDHVVHTAHVANTSSRAYQLNEHRMRGYLKEYNLTKDEISAVIEGVFAFWSEALSIEDKFESDRDKCDLLSEIEEVPRFGQWRSYYEIAWNGIDFYNGSSGGEFCDFYYVELDKVCAKHTEAVANYLAMRFLYRMDDKLKEPKQPEDQKVNCLMIVQMTLPNLFNRLYMAEHFTDEKHSEISDMVTEMRKAQRQILESVEWLDAETRAEALSKEAGITPVIGSYKNEELSDTLIREINNLTIVEGSYAQSLINLRVLGTYLHRYAGLHADELPKDTKPLTLLVGMQVNAFYYNLDNSVYVMAGVLHPPAYHRSWPDSLKFGTIGYLIGHELTHGFDTEGSSFDSAGEVRDWWSRKSEAVFQERAQCYVDYFNQYLIPEINQTIRGNETRDENIADAGGLRAALTGYRNHMKQLQRSGADGETLAHKNEQMPGLDLSPEQLFFVGFAQLWCADYEPEHYWEELTGAHTVDKYRVLGAVSNNDDFAEVYKCPLGSPMHPKAESCRMW

Target ORF information:

RefSeq Version	NM_143346.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neprilysin 7 (Nep7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143346.3

ORF Insert Sequence:

ATGCTCTCAA TCTCGAGCTA CTTTCGACTG CCCCTATACA CGCGGCTGGC AGTTTGCATC 
CTGATCTGCG TGATTTTGAA TGACTGCGAG GCCAGGAGTA TTGGCGACGA GTCCCATGAG 
AACGCGATTA CGGATGAGAC GGCCGACTAC CTGCGATCCC ATGGCGCCAT GATGAAGTCC 
TATATGAACT TCAGCGTCGA TCCGTGCGAC GACTTCTACG AGTTCGCGTG CGGCAACTGG 
AAGAACGTGA AAACGCCGCG GCAATCGCCC CATAGGCGGA GCAATATTCT GGACATCAGC 
TACACACTGA TGGACGTGAC GGAGCAGCTG CTGACCAGGA CAAAGTTGGC GGAGGCACTG 
AACGTGTCCG CCGAGCTGCT CGTTGCACAA CGCTTCTACA ATTCCTGCCT GGCGGCCGAG 
CTCTTTCCGC GGCCCGCTGC CGATCCCGCT TATCTATCGG TTATCAGGTC GCTGGGTGGT 
TTTCCCGCCG TCGACAGAGA CGCCTGGAAC GCCTCCAGCT TCAGTTGGTT CAACATGAGC 
GCCCATCTCA GCAACTACGG CGTTCGCAGT TTGATCCACG AAGAAATTCT GCCGCAGCAT 
CCCTTCAATC CGTACTTCAA GCTACCCGAA CTGGGCTTTG ACCATGTCGT CCACACCGCT 
CACGTTGCCA ATACGAGCTC GCGGGCCTAC CAGCTCAACG AGCATCGCAT GCGTGGCTAC 
CTGAAGGAAT ACAATCTCAC GAAGGATGAA ATCTCTGCCG TGATCGAAGG CGTCTTTGCT 
TTTTGGAGCG AGGCGCTCAG TATTGAGGAT AAGTTCGAGA GCGATAGGGA TAAGTGCGAT 
TTGCTCAGTG AGATTGAGGA GGTGCCGCGA TTTGGTCAGT GGAGGAGCTA CTACGAGATA 
GCCTGGAATG GTATAGACTT TTACAACGGC TCGTCCGGTG GCGAATTCTG CGACTTTTAC 
TATGTGGAGC TCGACAAGGT GTGCGCCAAG CATACGGAGG CGGTGGCCAA TTATCTAGCC 
ATGCGGTTTC TCTACCGCAT GGACGACAAG CTGAAGGAGC CCAAGCAGCC GGAGGACCAA 
AAGGTTAACT GCTTGATGAT CGTCCAGATG ACGCTGCCAA ATCTGTTCAA CAGGCTCTAC 
ATGGCGGAGC ACTTCACCGA CGAGAAGCAC TCGGAGATCT CTGACATGGT GACCGAAATG 
CGAAAGGCCC AGCGACAGAT CCTCGAGAGT GTGGAGTGGT TGGACGCGGA GACGCGAGCA 
GAGGCTCTGA GCAAGGAGGC CGGCATCACG CCAGTAATCG GCAGTTACAA AAACGAAGAG 
CTGTCGGACA CTCTAATCCG CGAGATCAAC AATCTGACCA TTGTTGAAGG CAGCTATGCC 
CAGAGTTTGA TCAATCTGCG AGTATTGGGC ACCTATCTTC ATCGCTACGC TGGCTTACAT 
GCGGACGAGC TGCCCAAGGA CACAAAGCCA CTGACACTGC TCGTGGGAAT GCAGGTGAAC 
GCCTTCTACT ACAATCTGGA TAACTCGGTG TACGTGATGG CGGGAGTACT GCATCCGCCT 
GCGTACCACC GTTCATGGCC CGATTCCTTG AAGTTCGGCA CCATTGGCTA TCTGATAGGT 
CACGAGTTGA CCCACGGCTT CGATACGGAG GGCTCCAGTT TCGACAGCGC CGGCGAAGTA 
CGCGACTGGT GGTCCAGGAA GTCGGAGGCG GTATTCCAGG AACGGGCGCA GTGCTATGTG 
GATTACTTCA ACCAATACTT GATACCGGAA ATCAACCAGA CAATTCGCGG CAATGAGACA 
AGGGACGAGA ACATAGCGGA CGCCGGTGGC TTGAGGGCCG CACTCACCGG CTATCGAAAC 
CACATGAAGC AGCTGCAACG AAGTGGAGCG GATGGGGAGA CGTTGGCTCA CAAAAACGAG 
CAGATGCCCG GCCTCGACCT GTCGCCGGAG CAGCTCTTCT TCGTGGGATT CGCGCAGCTG 
TGGTGCGCCG ACTACGAGCC GGAGCACTAC TGGGAGGAGC TCACTGGCGC ACATACCGTC 
GATAAGTATC GGGTGCTGGG TGCCGTTTCC AACAACGACG ATTTTGCTGA GGTCTACAAG 
TGCCCACTGG GCAGTCCAAT GCATCCGAAG GCTGAGAGTT GTCGGATGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nep7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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