Vps33B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps33B
Entrez Gene ID	43077
Full Name	Vacuolar protein sorting 33B
Synonyms	CG5127,Dmel\CG5127,VPS33b,Vps 33B,Vps33b,dVps33B
General protein information	Preferred Names Vacuolar protein sorting 33B Names CG5127 gene product from transcript CG5127-RA CG5127-PA Vps33B-PA vacuolar protein sorting 33B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96E1-96E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_143138.3	NP_651395.1	vacuolar protein sorting 33B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Vps33B	NP_651395.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001463	XP_321664.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that Vps33B function is critical for determining the fate of signaling endosomes formed following pattern-recognition receptors activation
Title: ARC Syndrome-Linked Vps33B Protein Is Required for Inflammatory Endosomal Maturation and Signal Termination.

The following Vps33B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps33B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06093	NM_143138.3 Latest version!	Drosophila melanogaster vacuolar protein sorting 33B (Vps33B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps33B ( NM_143138.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651395.1 Drosophila melanogaster vacuolar protein sorting 33B (Vps33B), mRNA.

Price & Availability↑

CloneID	OFa06093
Clone ID Related Accession (Same CDS sequence)	NM_143138.3
Accession Version	NM_143138.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar protein sorting 33B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143138.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTTGA CGCTGGACAA AAAGCTGCAG GGATTCCAGT TGGTGGCACA AGAAAAGCTT 
TGTTCCATAC TATGTAGCAT ACCTGGAAAG AAGGAGTTAA TTTTGGAGCC CGACTTGATA 
AAGCCACTGG AACATGTTGT CACTGCCTCC TGGCTGAAGT TGAAGGGCAT ACAACGGATC 
TACAAACATG ATGCGGCACA ATCTTTGCCC CGATCCGCCG ATCAGGTGCA CATCTACATG 
ATCCGCAGTG TTTTGGGTAC ATTTCAGACA CTTCTCAAGC AATTGCAACC CGTGGCCTCA 
GAGGAAATGC CCGACATCTC CATGAAAATG TATCACATAG TCTGTGTGCC CAGTTGTTAC 
TCCTATTTTC AAACCCTGCT GGAACAGGCT GGTTTGTACG GACTGGTGCA ACTTCATCAC 
TTTAACTGGG ACTTCATCTA TTTTGATCAG GGAGTTCTCA GCTTGGAGCT GCCCAATCTT 
TACGAGTGTC TCTACCTGCA GAAGAACACA TCTCCATTGC CTGCGGTGGC CCAGAGCCTG 
CGATTGCTTC AGATGATTTG CGGACAACCG TCTTTGATTC TGAGCTTTGG CCACCACTCG 
TCGCAGCTAA TGCAAATGGC GAAGACTCTT GGCAAACTTC CGGCGCCCAC AAATCCTCCA 
GACTATGGCG GCTGGCTGGT CATCGATCGG GATAAGGATT ACCCCGCCAG CTTGCTCACT 
CCGGCCATCT ATGCAGGTCT TCTCTTGGAA GTTTTCGAGC ATAGTTCTGG CGAGATTCTG 
GTAGACAATT CCAAGAACAA GATCCGCAGC CAGCGTGTGG AGTTACTCCA GGGAAAGAAA 
TCCAAGATAG GCGTAAACTC AGCCAGCAAG CCGTGTTCCA TTCGTCTTAA CTCCACATCC 
GATGAGATCT ACGGCGATAA TCGGTACAAA CGATTTGCTC AGGTCAGCAG TTTGATTCAC 
GCACAAGTAA AAGCCCTGGG CTTGGAACTG CAGAAACTCA ATGACATGCA GCTGGACGAG 
ATGCATGACT ATGTGGCCAG GAAGCTACCC AAACTAACCG AGCTTAAGAG CAAAGTCCTT 
AGGCATCTGA ATGCCAGCGA GATTGTGATC CAAATGATGG GTAACTTCAG GAAGCTCCAA 
ACGCTGGAGG AGGACATACT AAACAATGAT TCAAGAAAGC GATTGCTAAG CGAGATCGAC 
GAGCTGTTGA CCACAGATGG TCAGAGATTT AATACACTGC GACTGCTGTG CCTCTTGCAT 
CATTGCGTTG GCGTGGCGCC CGAGGAGCTG CAGATATTTG CGCGGAACTA CTGCAATCTG 
TTTGGACACC AGGAATTGGG CGTGTTCCAG CAGTTGTCGC AGGCGGGCTT GTTGCCACCA 
CTTGTGGTGG AGAAAAAGGC GCCAACGAAA CTGCTCTCAA ATCTACCTCT GCCCAAGTTC 
CAGCAAACGG AGTTTCAGGC CAATGCTAAT CGGCTAAAGC TGTTGACCTC CAGTGGAGAT 
GGTCAGGATG GAGGTTCCTC CTCCAAGAGC CAATCCAGTG GCTTGCAGAC CTGTCCCAGT 
TTTGTGTTCA ATGGCACCTA CATACCATTG GTGGCTCAGC TCTGCTCCAT TTTGCTGAAA 
ATCAATAGTG CTGAGGAGTT GTCCTCGAAG CTGGGCATGA TTGAGGGGCT GCATCTACAC 
CTGGACAGCG GAAAAATCAC GCCCAAGGCC TATGCCAGCC AAGTGAAGCT AAACGGAGTT 
GCAGATCAGG ATATGTTCCC CTTGCGTCTC AGGAATCTCT TTGTATTCAT TGTGGGTGGT 
GCCAGTTATG CGGAAATCGC CGCCTGCGAT TTTGTGGCAA AGCTAACAGG CGCCCAAATA 
ACCGTGGCAT CCGACTCTCT AATGGCGGGC AGTGATTTGA TCGCCACCGC CTTCAACCGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651395.1
CDS	27..1949
Translation	MDLTLDKKLQGFQLVAQEKLCSILCSIPGKKELILEPDLIKPLEHVVTASWLKLKGIQRIYKHDAAQSLPRSADQVHIYMIRSVLGTFQTLLKQLQPVASEEMPDISMKMYHIVCVPSCYSYFQTLLEQAGLYGLVQLHHFNWDFIYFDQGVLSLELPNLYECLYLQKNTSPLPAVAQSLRLLQMICGQPSLILSFGHHSSQLMQMAKTLGKLPAPTNPPDYGGWLVIDRDKDYPASLLTPAIYAGLLLEVFEHSSGEILVDNSKNKIRSQRVELLQGKKSKIGVNSASKPCSIRLNSTSDEIYGDNRYKRFAQVSSLIHAQVKALGLELQKLNDMQLDEMHDYVARKLPKLTELKSKVLRHLNASEIVIQMMGNFRKLQTLEEDILNNDSRKRLLSEIDELLTTDGQRFNTLRLLCLLHHCVGVAPEELQIFARNYCNLFGHQELGVFQQLSQAGLLPPLVVEKKAPTKLLSNLPLPKFQQTEFQANANRLKLLTSSGDGQDGGSSSKSQSSGLQTCPSFVFNGTYIPLVAQLCSILLKINSAEELSSKLGMIEGLHLHLDSGKITPKAYASQVKLNGVADQDMFPLRLRNLFVFIVGGASYAEIAACDFVAKLTGAQITVASDSLMAGSDLIATAFNR

Target ORF information:

RefSeq Version	NM_143138.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar protein sorting 33B (Vps33B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143138.3

ORF Insert Sequence:

ATGGATTTGA CGCTGGACAA AAAGCTGCAG GGATTCCAGT TGGTGGCACA AGAAAAGCTT 
TGTTCCATAC TATGTAGCAT ACCTGGAAAG AAGGAGTTAA TTTTGGAGCC CGACTTGATA 
AAGCCACTGG AACATGTTGT CACTGCCTCC TGGCTGAAGT TGAAGGGCAT ACAACGGATC 
TACAAACATG ATGCGGCACA ATCTTTGCCC CGATCCGCCG ATCAGGTGCA CATCTACATG 
ATCCGCAGTG TTTTGGGTAC ATTTCAGACA CTTCTCAAGC AATTGCAACC CGTGGCCTCA 
GAGGAAATGC CCGACATCTC CATGAAAATG TATCACATAG TCTGTGTGCC CAGTTGTTAC 
TCCTATTTTC AAACCCTGCT GGAACAGGCT GGTTTGTACG GACTGGTGCA ACTTCATCAC 
TTTAACTGGG ACTTCATCTA TTTTGATCAG GGAGTTCTCA GCTTGGAGCT GCCCAATCTT 
TACGAGTGTC TCTACCTGCA GAAGAACACA TCTCCATTGC CTGCGGTGGC CCAGAGCCTG 
CGATTGCTTC AGATGATTTG CGGACAACCG TCTTTGATTC TGAGCTTTGG CCACCACTCG 
TCGCAGCTAA TGCAAATGGC GAAGACTCTT GGCAAACTTC CGGCGCCCAC AAATCCTCCA 
GACTATGGCG GCTGGCTGGT CATCGATCGG GATAAGGATT ACCCCGCCAG CTTGCTCACT 
CCGGCCATCT ATGCAGGTCT TCTCTTGGAA GTTTTCGAGC ATAGTTCTGG CGAGATTCTG 
GTAGACAATT CCAAGAACAA GATCCGCAGC CAGCGTGTGG AGTTACTCCA GGGAAAGAAA 
TCCAAGATAG GCGTAAACTC AGCCAGCAAG CCGTGTTCCA TTCGTCTTAA CTCCACATCC 
GATGAGATCT ACGGCGATAA TCGGTACAAA CGATTTGCTC AGGTCAGCAG TTTGATTCAC 
GCACAAGTAA AAGCCCTGGG CTTGGAACTG CAGAAACTCA ATGACATGCA GCTGGACGAG 
ATGCATGACT ATGTGGCCAG GAAGCTACCC AAACTAACCG AGCTTAAGAG CAAAGTCCTT 
AGGCATCTGA ATGCCAGCGA GATTGTGATC CAAATGATGG GTAACTTCAG GAAGCTCCAA 
ACGCTGGAGG AGGACATACT AAACAATGAT TCAAGAAAGC GATTGCTAAG CGAGATCGAC 
GAGCTGTTGA CCACAGATGG TCAGAGATTT AATACACTGC GACTGCTGTG CCTCTTGCAT 
CATTGCGTTG GCGTGGCGCC CGAGGAGCTG CAGATATTTG CGCGGAACTA CTGCAATCTG 
TTTGGACACC AGGAATTGGG CGTGTTCCAG CAGTTGTCGC AGGCGGGCTT GTTGCCACCA 
CTTGTGGTGG AGAAAAAGGC GCCAACGAAA CTGCTCTCAA ATCTACCTCT GCCCAAGTTC 
CAGCAAACGG AGTTTCAGGC CAATGCTAAT CGGCTAAAGC TGTTGACCTC CAGTGGAGAT 
GGTCAGGATG GAGGTTCCTC CTCCAAGAGC CAATCCAGTG GCTTGCAGAC CTGTCCCAGT 
TTTGTGTTCA ATGGCACCTA CATACCATTG GTGGCTCAGC TCTGCTCCAT TTTGCTGAAA 
ATCAATAGTG CTGAGGAGTT GTCCTCGAAG CTGGGCATGA TTGAGGGGCT GCATCTACAC 
CTGGACAGCG GAAAAATCAC GCCCAAGGCC TATGCCAGCC AAGTGAAGCT AAACGGAGTT 
GCAGATCAGG ATATGTTCCC CTTGCGTCTC AGGAATCTCT TTGTATTCAT TGTGGGTGGT 
GCCAGTTATG CGGAAATCGC CGCCTGCGAT TTTGTGGCAA AGCTAACAGG CGCCCAAATA 
ACCGTGGCAT CCGACTCTCT AATGGCGGGC AGTGATTTGA TCGCCACCGC CTTCAACCGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vps33B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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