stac cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	stac
Entrez Gene ID	43002
Full Name	staccato
Synonyms	BP1013,CG11819,CG34037,CG34349,Dmel\CG34349,Dmel_CG11819,Dmel_CG34037,Unc-13-4B,dunc-13-4B,unc-13-4B
General protein information	Preferred Names staccato Names CG34349 gene product from transcript CG34349-RE CG34349-PC CG34349-PD CG34349-PE Unc-13 ortholog 4B Uncoordinated-13 4B stac-PC stac-PD stac-PE unc-13-4B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96B-96B

mRNA and Protein(s)

mRNA	Protein	Name
NM_001104439.3	NP_001097909.2	staccato, isoform D
NM_143072.4	NP_651329.4	staccato, isoform C
NM_001276018.1	NP_001262947.1	staccato, isoform E

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG34349	NP_651329.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003164	XP_003436413.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GTPase Arl3 is necessary and sufficient for Stac lysosome-related organelle formation and promotes Stac-dependent intracellular fusion of juxtaposed apical plasma membranes
Title: Staccato/Unc-13-4 controls secretory lysosome-mediated lumen fusion during epithelial tube?anastomosis.

The following stac gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the stac cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06057	NM_001276018.1 Latest version!	Drosophila melanogaster staccato (stac), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa08796	NM_143072.4 Latest version!	Drosophila melanogaster staccato (stac), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa08797	NM_001104439.3 Latest version!	Drosophila melanogaster staccato (stac), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

stac ( NM_001276018.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262947.1 Drosophila melanogaster staccato (stac), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa06057
Clone ID Related Accession (Same CDS sequence)	NM_001276018.1
Accession Version	NM_001276018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	staccato, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAAAAA GTTACTTCCG GCGCTCGGAA TTAAGGATAT TTGAAAAAAT AAGACTGCAA 
GACGGAGATG ATGGATTTTT CGAAAAGTTT GGATCCCTAC TGCGGCAGAA GTCCCAAATC 
GATGAGGGCG TCCTCAAAAG TTCACTGGCT CCCTTGGAGG AAAATGGCAG CGGCGGAGAA 
GAAGATTCGG ACGAGAGTCC CGATGGCACT TTGCAACTCA GCCAGGATTC GGAGTGCACC 
AGCATCGATA CCTTTGCCAC CGAGAGTACA CTGGCGTCCA GTTCGGACAA GGATATCAGC 
ACTTCAGTTC TGGACTCGGC GCCAGTGATG AATGTCACAG ATCTCTATGA AGAGATATTA 
TTTGAAATTT TCAACAACAT TGGCTGTGAA AACAACGAAG AGTGCACAAA CAGTTTGGTC 
GAATTCGTGC AGGATGCATT CAAAATTCCG AACGCCACTC ATGAGGAAAT TTATGAGGCG 
GCGCGACTGA AAGAACCGCC CAATGTTAGA CTAAATGTCG AGATTATAAA GGCCGAGAAC 
CTCATGTCCA AGGACTCAAA CGGCTTGTCC GATCCGTTTG TTACCCTGTA CTTGGAATCG 
AATGGCTCCC ATCGATACAA CTCATCGGTC AAGCCTGCCA CATTGAATCC CATATGGGAG 
GAGCACTTTT CACTGCCAAT TACTGAAAAT GCTCGAGACG AAGTTCTTAT CGTTGAAGTG 
TGGGACTTTG ATGCTGCGGA AACCGTCAAG GAAAAAGTCA ACAAAATCCT GGATGTTAAG 
GGTGTGAAAG GACTTAGCAA GCTGATGAAG GAAATTGCTG TAACAGCTTC CTCGGGAAAA 
CATGATAATG AACTGATTGG GAGAGCTGCA ATTACATTGA AGTCTATTCC CGTTTCCGGC 
CTCACCGTAT GGTATAACCT GGAAAAGGGA AGCAAGGGAC GCAGTCGAGG ATCACTTCTG 
GTTAATCTGG CCTTGAGTGC GGAGAAGAAC AAAAGTGTCG CCGTTCAGGA GCACAAGAAC 
CTACTGAAAC TGCTCCTGAT GTACGAGCTG GAAACCTCGC AGGTGGCCAA CTACTGGTGG 
AGCGGCAAGT TCAGTCCCAA TGCCGAACTC ATTCGATCCC AGCATGCTGC CCAGAGTGGG 
CTAACTCCTT TCGACTGTGC GCTCAGCCAG TGGCACGCGT ACAGCACCAT CCATGAGACG 
CACAAACTCA ATTTCACACT ATTCAATTCA ATTCTGGACG TGGTGGTGCC AGTGATTACT 
TACATGCAGA ACGACTCCGA GGATGTGAAA ACATTCTGGG ATGGCGTGAA ACGACTGCTT 
CCCTCGTGCT TCGCAGTGCT AAGGAAACTA AGATCCAAAA ACACCAGTGA TAAAAACATA 
ATTAGAGCTC TGAACGAAGT TCTGGATATT CTAAAGAAGA TTAAGGAGCT GGAAGTGCCG 
GAGAGTGTAG ACATATTCCC CAAATCGGTG TATGGTTGGC TACACACAAA CGACACTGAT 
GAAACGTGCA ATATTGATAC TGCCATTGAG GATGCTATCA ACACGGGGAC AAGGGAATGG 
CTGGAGCACA TAGTGGAGGG CAGCAGGCAA TCTAAGAACA CCGAAACCGA TGACGAAAAG 
CTGCAGTACG TCATTAAACT GATACAAATG GTGCGGTCTG ACCTGCAACG TGCAATGGAA 
TACTTCGATA AAATCTTTTA CCACAAAATT CAGCTTAACT ATTCCGCTGT ACTTTATTTA 
TTTTATGACT CCAAGCTTGC TGAAATCTGT AAATCGATTA TAATAGAAGT GTGCAATAAT 
ATTAAGAGAT TGGATGTGCC AGACGATCAA TTCGAATATT TACCCAACCT TGAGAATGTA 
AATATGGGCA CGACTTTGTT TGAAGTCTAT TTAATACTCA AACGTTACGT TCAATTAGGA 
GAATCTCTTT GCTCCGAGCC CCTGGAACTG TCCAACTTTT ACCCGTGGTT CGAGAGGGGA 
GTTACCCACT GGCTGGATAT TTCAATAATT AAGGCATTGA GTCGCATCCA GAAAGCAATT 
GATTTGGATC AGCTGAAAGC GGTCGATGAA ACAGTGAAAT ACAGCTCCTC GGCAGTGGAC 
ACACTTTCCA TATTCTACCA AATCAAGATA TTTTGGCAAC AACTTGACTG GCCAGAGGTC 
GAGGGTTCCT ACATCTTCGT AGCCAAAATC GTCAATGATC TTTGTCGCTG CTGCATATTT 
TATGCCCAAC AGATGTCCCG CAGAGTTGAG AATATTTTTA TTGCTGATGA TAACAATAAA 
AATTTCATTT TGTCGGAGGA GTGGTGCATA GCGATAAACA ACATGGATTA CATCCGGCAG 
AGCTTACCTT CATTCATAAA GGAACTTAGC ATTGATGACA TTATCAAGAG ATTGGGGGAG 
TATCGAACTA ACTTAGAGGC TGAGAGATGC GCAAGCACCA TCAAAACTGT CATTGAAAAT 
GCATTGGACA CCGAACGCAA TCAGATTGTT GAGCTCATAG AGATTGTGGC CAGGAAAATG 
GCACCGCCAA TTAGACGATA CTTGGCCGAA GGTGCTGAGG TGCTCGCCAA GGACTCCAAC 
TCCATGGACC AGTTAATGAT GTACCTGGAA TCCTCATTGG CCACACTCTA CGATACTCTC 
AATGAGATTA ATTTCCAGAG GATACTGGAT GGCATTTGGT CAGAGTTATC CATTATAATG 
TATGATCTAA TTCAATCAAA TCTTGACAAA CGCCGACCAC CAGCTTTCTT CCAAAATTTG 
AACAATACTC TTCAGACAAT GATGGACTGC TTTAAAATGG GCAACCTTCA AACTTCAGAC 
ATAAAAATAT TGTCTTCAAT TCAAAGTCGA CTAAGGCTTT ATTCCCTGGA GACATCGGAT 
CTTATTCATC AGTATTACTT GGAGCGTCTA GAAAACCAAA AGAGCCAAGA GTCATCTCCA 
TATGGACAAC TTACCATCAC GGCTCAGTTA ACTGATACCG GCTTATTGTT AAATATCTTA 
AATGCTCGCA ATCTGCTGCC AATGGATTCA AATGGTTCGG TGGACTCATT TGTTAAGGCC 
AGTTTCATGC CCACAAGTCG ATTCAACGAC GTACCCACTG TGAAGACCAA CGTGCACAAC 
AAAAGTTGTT TTCCGCTTTA CGATCAGGAG TTTCGTATAA ACTTGAGTGA CCACCAGCGA 
AGTGAAAAGA ATAGTCTTAT TGTATTTAGC ATCAAAGATA AAGATTTATT CGGCATGTCG 
AGCCAGTATA TAGCAGAGAG CTATATATCT TTCGCAGATC TGGAAGCGAC ACCACCGGGC 
GAGCAAATAA TGATGAATCT ATCCAGACCA GAGTATACAG ATTCCGAATC ACTGCGTGCC 
TTGGAATACA GACTAGGTGA TAAGCAGGCC AAGGATTTCC TTAAAAAGCT GAAGAACCGA 
TCCTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262947.1
CDS	161..3592
Translation	MLKSYFRRSELRIFEKIRLQDGDDGFFEKFGSLLRQKSQIDEGVLKSSLAPLEENGSGGEEDSDESPDGTLQLSQDSECTSIDTFATESTLASSSDKDISTSVLDSAPVMNVTDLYEEILFEIFNNIGCENNEECTNSLVEFVQDAFKIPNATHEEIYEAARLKEPPNVRLNVEIIKAENLMSKDSNGLSDPFVTLYLESNGSHRYNSSVKPATLNPIWEEHFSLPITENARDEVLIVEVWDFDAAETVKEKVNKILDVKGVKGLSKLMKEIAVTASSGKHDNELIGRAAITLKSIPVSGLTVWYNLEKGSKGRSRGSLLVNLALSAEKNKSVAVQEHKNLLKLLLMYELETSQVANYWWSGKFSPNAELIRSQHAAQSGLTPFDCALSQWHAYSTIHETHKLNFTLFNSILDVVVPVITYMQNDSEDVKTFWDGVKRLLPSCFAVLRKLRSKNTSDKNIIRALNEVLDILKKIKELEVPESVDIFPKSVYGWLHTNDTDETCNIDTAIEDAINTGTREWLEHIVEGSRQSKNTETDDEKLQYVIKLIQMVRSDLQRAMEYFDKIFYHKIQLNYSAVLYLFYDSKLAEICKSIIIEVCNNIKRLDVPDDQFEYLPNLENVNMGTTLFEVYLILKRYVQLGESLCSEPLELSNFYPWFERGVTHWLDISIIKALSRIQKAIDLDQLKAVDETVKYSSSAVDTLSIFYQIKIFWQQLDWPEVEGSYIFVAKIVNDLCRCCIFYAQQMSRRVENIFIADDNNKNFILSEEWCIAINNMDYIRQSLPSFIKELSIDDIIKRLGEYRTNLEAERCASTIKTVIENALDTERNQIVELIEIVARKMAPPIRRYLAEGAEVLAKDSNSMDQLMMYLESSLATLYDTLNEINFQRILDGIWSELSIIMYDLIQSNLDKRRPPAFFQNLNNTLQTMMDCFKMGNLQTSDIKILSSIQSRLRLYSLETSDLIHQYYLERLENQKSQESSPYGQLTITAQLTDTGLLLNILNARNLLPMDSNGSVDSFVKASFMPTSRFNDVPTVKTNVHNKSCFPLYDQEFRINLSDHQRSEKNSLIVFSIKDKDLFGMSSQYIAESYISFADLEATPPGEQIMMNLSRPEYTDSESLRALEYRLGDKQAKDFLKKLKNRSFS

Target ORF information:

RefSeq Version	NM_001276018.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster staccato (stac), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276018.1

ORF Insert Sequence:

ATGTTAAAAA GTTACTTCCG GCGCTCGGAA TTAAGGATAT TTGAAAAAAT AAGACTGCAA 
GACGGAGATG ATGGATTTTT CGAAAAGTTT GGATCCCTAC TGCGGCAGAA GTCCCAAATC 
GATGAGGGCG TCCTCAAAAG TTCACTGGCT CCCTTGGAGG AAAATGGCAG CGGCGGAGAA 
GAAGATTCGG ACGAGAGTCC CGATGGCACT TTGCAACTCA GCCAGGATTC GGAGTGCACC 
AGCATCGATA CCTTTGCCAC CGAGAGTACA CTGGCGTCCA GTTCGGACAA GGATATCAGC 
ACTTCAGTTC TGGACTCGGC GCCAGTGATG AATGTCACAG ATCTCTATGA AGAGATATTA 
TTTGAAATTT TCAACAACAT TGGCTGTGAA AACAACGAAG AGTGCACAAA CAGTTTGGTC 
GAATTCGTGC AGGATGCATT CAAAATTCCG AACGCCACTC ATGAGGAAAT TTATGAGGCG 
GCGCGACTGA AAGAACCGCC CAATGTTAGA CTAAATGTCG AGATTATAAA GGCCGAGAAC 
CTCATGTCCA AGGACTCAAA CGGCTTGTCC GATCCGTTTG TTACCCTGTA CTTGGAATCG 
AATGGCTCCC ATCGATACAA CTCATCGGTC AAGCCTGCCA CATTGAATCC CATATGGGAG 
GAGCACTTTT CACTGCCAAT TACTGAAAAT GCTCGAGACG AAGTTCTTAT CGTTGAAGTG 
TGGGACTTTG ATGCTGCGGA AACCGTCAAG GAAAAAGTCA ACAAAATCCT GGATGTTAAG 
GGTGTGAAAG GACTTAGCAA GCTGATGAAG GAAATTGCTG TAACAGCTTC CTCGGGAAAA 
CATGATAATG AACTGATTGG GAGAGCTGCA ATTACATTGA AGTCTATTCC CGTTTCCGGC 
CTCACCGTAT GGTATAACCT GGAAAAGGGA AGCAAGGGAC GCAGTCGAGG ATCACTTCTG 
GTTAATCTGG CCTTGAGTGC GGAGAAGAAC AAAAGTGTCG CCGTTCAGGA GCACAAGAAC 
CTACTGAAAC TGCTCCTGAT GTACGAGCTG GAAACCTCGC AGGTGGCCAA CTACTGGTGG 
AGCGGCAAGT TCAGTCCCAA TGCCGAACTC ATTCGATCCC AGCATGCTGC CCAGAGTGGG 
CTAACTCCTT TCGACTGTGC GCTCAGCCAG TGGCACGCGT ACAGCACCAT CCATGAGACG 
CACAAACTCA ATTTCACACT ATTCAATTCA ATTCTGGACG TGGTGGTGCC AGTGATTACT 
TACATGCAGA ACGACTCCGA GGATGTGAAA ACATTCTGGG ATGGCGTGAA ACGACTGCTT 
CCCTCGTGCT TCGCAGTGCT AAGGAAACTA AGATCCAAAA ACACCAGTGA TAAAAACATA 
ATTAGAGCTC TGAACGAAGT TCTGGATATT CTAAAGAAGA TTAAGGAGCT GGAAGTGCCG 
GAGAGTGTAG ACATATTCCC CAAATCGGTG TATGGTTGGC TACACACAAA CGACACTGAT 
GAAACGTGCA ATATTGATAC TGCCATTGAG GATGCTATCA ACACGGGGAC AAGGGAATGG 
CTGGAGCACA TAGTGGAGGG CAGCAGGCAA TCTAAGAACA CCGAAACCGA TGACGAAAAG 
CTGCAGTACG TCATTAAACT GATACAAATG GTGCGGTCTG ACCTGCAACG TGCAATGGAA 
TACTTCGATA AAATCTTTTA CCACAAAATT CAGCTTAACT ATTCCGCTGT ACTTTATTTA 
TTTTATGACT CCAAGCTTGC TGAAATCTGT AAATCGATTA TAATAGAAGT GTGCAATAAT 
ATTAAGAGAT TGGATGTGCC AGACGATCAA TTCGAATATT TACCCAACCT TGAGAATGTA 
AATATGGGCA CGACTTTGTT TGAAGTCTAT TTAATACTCA AACGTTACGT TCAATTAGGA 
GAATCTCTTT GCTCCGAGCC CCTGGAACTG TCCAACTTTT ACCCGTGGTT CGAGAGGGGA 
GTTACCCACT GGCTGGATAT TTCAATAATT AAGGCATTGA GTCGCATCCA GAAAGCAATT 
GATTTGGATC AGCTGAAAGC GGTCGATGAA ACAGTGAAAT ACAGCTCCTC GGCAGTGGAC 
ACACTTTCCA TATTCTACCA AATCAAGATA TTTTGGCAAC AACTTGACTG GCCAGAGGTC 
GAGGGTTCCT ACATCTTCGT AGCCAAAATC GTCAATGATC TTTGTCGCTG CTGCATATTT 
TATGCCCAAC AGATGTCCCG CAGAGTTGAG AATATTTTTA TTGCTGATGA TAACAATAAA 
AATTTCATTT TGTCGGAGGA GTGGTGCATA GCGATAAACA ACATGGATTA CATCCGGCAG 
AGCTTACCTT CATTCATAAA GGAACTTAGC ATTGATGACA TTATCAAGAG ATTGGGGGAG 
TATCGAACTA ACTTAGAGGC TGAGAGATGC GCAAGCACCA TCAAAACTGT CATTGAAAAT 
GCATTGGACA CCGAACGCAA TCAGATTGTT GAGCTCATAG AGATTGTGGC CAGGAAAATG 
GCACCGCCAA TTAGACGATA CTTGGCCGAA GGTGCTGAGG TGCTCGCCAA GGACTCCAAC 
TCCATGGACC AGTTAATGAT GTACCTGGAA TCCTCATTGG CCACACTCTA CGATACTCTC 
AATGAGATTA ATTTCCAGAG GATACTGGAT GGCATTTGGT CAGAGTTATC CATTATAATG 
TATGATCTAA TTCAATCAAA TCTTGACAAA CGCCGACCAC CAGCTTTCTT CCAAAATTTG 
AACAATACTC TTCAGACAAT GATGGACTGC TTTAAAATGG GCAACCTTCA AACTTCAGAC 
ATAAAAATAT TGTCTTCAAT TCAAAGTCGA CTAAGGCTTT ATTCCCTGGA GACATCGGAT 
CTTATTCATC AGTATTACTT GGAGCGTCTA GAAAACCAAA AGAGCCAAGA GTCATCTCCA 
TATGGACAAC TTACCATCAC GGCTCAGTTA ACTGATACCG GCTTATTGTT AAATATCTTA 
AATGCTCGCA ATCTGCTGCC AATGGATTCA AATGGTTCGG TGGACTCATT TGTTAAGGCC 
AGTTTCATGC CCACAAGTCG ATTCAACGAC GTACCCACTG TGAAGACCAA CGTGCACAAC 
AAAAGTTGTT TTCCGCTTTA CGATCAGGAG TTTCGTATAA ACTTGAGTGA CCACCAGCGA 
AGTGAAAAGA ATAGTCTTAT TGTATTTAGC ATCAAAGATA AAGATTTATT CGGCATGTCG 
AGCCAGTATA TAGCAGAGAG CTATATATCT TTCGCAGATC TGGAAGCGAC ACCACCGGGC 
GAGCAAATAA TGATGAATCT ATCCAGACCA GAGTATACAG ATTCCGAATC ACTGCGTGCC 
TTGGAATACA GACTAGGTGA TAAGCAGGCC AAGGATTTCC TTAAAAAGCT GAAGAACCGA 
TCCTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stac ( NM_143072.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651329.4 Drosophila melanogaster staccato (stac), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa08796
Clone ID Related Accession (Same CDS sequence)	NM_143072.4
Accession Version	NM_143072.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	staccato, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143072.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGAGG AGCAGATGTG GAAGGGCTTC TTCTTCAAGT TGCAGGAGCT CAAAATGAAT 
CCACCCACGG AGCATGAACA CCAACTGCAA GACGGAGATG ATGGATTTTT CGAAAAGTTT 
GGATCCCTAC TGCGGCAGAA GTCCCAAATC GATGAGGGCG TCCTCAAAAG TTCACTGGCT 
CCCTTGGAGG AAAATGGCAG CGGCGGAGAA GAAGATTCGG ACGAGAGTCC CGATGGCACT 
TTGCAACTCA GCCAGGATTC GGAGTGCACC AGCATCGATA CCTTTGCCAC CGAGAGTACA 
CTGGCGTCCA GTTCGGACAA GGATATCAGC ACTTCAGTTC TGGACTCGGC GCCAGTGATG 
AATGTCACAG ATCTCTATGA AGAGATATTA TTTGAAATTT TCAACAACAT TGGCTGTGAA 
AACAACGAAG AGTGCACAAA CAGTTTGGTC GAATTCGTGC AGGATGCATT CAAAATTCCG 
AACGCCACTC ATGAGGAAAT TTATGAGGCG GCGCGACTGA AAGAACCGCC CAATGTTAGA 
CTAAATGTCG AGATTATAAA GGCCGAGAAC CTCATGTCCA AGGACTCAAA CGGCTTGTCC 
GATCCGTTTG TTACCCTGTA CTTGGAATCG AATGGCTCCC ATCGATACAA CTCATCGGTC 
AAGCCTGCCA CATTGAATCC CATATGGGAG GAGCACTTTT CACTGCCAAT TACTGAAAAT 
GCTCGAGACG AAGTTCTTAT CGTTGAAGTG TGGGACTTTG ATGCTGCGGA AACCGTCAAG 
GAAAAAGTCA ACAAAATCCT GGATGTTAAG GGTGTGAAAG GACTTAGCAA GCTGATGAAG 
GAAATTGCTG TAACAGCTTC CTCGGGAAAA CATGATAATG AACTGATTGG GAGAGCTGCA 
ATTACATTGA AGTCTATTCC CGTTTCCGGC CTCACCGTAT GGTATAACCT GGAAAAGGGA 
AGCAAGGGAC GCAGTCGAGG ATCACTTCTG GTTAATCTGG CCTTGAGTGC GGAGAAGAAC 
AAAAGTGTCG CCGTTCAGGA GCACAAGAAC CTACTGAAAC TGCTCCTGAT GTACGAGCTG 
GAAACCTCGC AGGTGGCCAA CTACTGGTGG AGCGGCAAGT TCAGTCCCAA TGCCGAACTC 
ATTCGATCCC AGCATGCTGC CCAGAGTGGG CTAACTCCTT TCGACTGTGC GCTCAGCCAG 
TGGCACGCGT ACAGCACCAT CCATGAGACG CACAAACTCA ATTTCACACT ATTCAATTCA 
ATTCTGGACG TGGTGGTGCC AGTGATTACT TACATGCAGA ACGACTCCGA GGATGTGAAA 
ACATTCTGGG ATGGCGTGAA ACGACTGCTT CCCTCGTGCT TCGCAGTGCT AAGGAAACTA 
AGATCCAAAA ACACCAGTGA TAAAAACATA ATTAGAGCTC TGAACGAAGT TCTGGATATT 
CTAAAGAAGA TTAAGGAGCT GGAAGTGCCG GAGAGTGTAG ACATATTCCC CAAATCGGTG 
TATGGTTGGC TACACACAAA CGACACTGAT GAAACGTGCA ATATTGATAC TGCCATTGAG 
GATGCTATCA ACACGGGGAC AAGGGAATGG CTGGAGCACA TAGTGGAGGG CAGCAGGCAA 
TCTAAGAACA CCGAAACCGA TGACGAAAAG CTGCAGTACG TCATTAAACT GATACAAATG 
GTGCGGTCTG ACCTGCAACG TGCAATGGAA TACTTCGATA AAATCTTTTA CCACAAAATT 
CAGCTTAACT ATTCCGCTGT ACTTTATTTA TTTTATGACT CCAAGCTTGC TGAAATCTGT 
AAATCGATTA TAATAGAAGT GTGCAATAAT ATTAAGAGAT TGGATGTGCC AGACGATCAA 
TTCGAATATT TACCCAACCT TGAGAATGTA AATATGGGCA CGACTTTGTT TGAAGTCTAT 
TTAATACTCA AACGTTACGT TCAATTAGGA GAATCTCTTT GCTCCGAGCC CCTGGAACTG 
TCCAACTTTT ACCCGTGGTT CGAGAGGGGA GTTACCCACT GGCTGGATAT TTCAATAATT 
AAGGCATTGA GTCGCATCCA GAAAGCAATT GATTTGGATC AGCTGAAAGC GGTCGATGAA 
ACAGTGAAAT ACAGCTCCTC GGCAGTGGAC ACACTTTCCA TATTCTACCA AATCAAGATA 
TTTTGGCAAC AACTTGACTG GCCAGAGGTC GAGGGTTCCT ACATCTTCGT AGCCAAAATC 
GTCAATGATC TTTGTCGCTG CTGCATATTT TATGCCCAAC AGATGTCCCG CAGAGTTGAG 
AATATTTTTA TTGCTGATGA TAACAATAAA AATTTCATTT TGTCGGAGGA GTGGTGCATA 
GCGATAAACA ACATGGATTA CATCCGGCAG AGCTTACCTT CATTCATAAA GGAACTTAGC 
ATTGATGACA TTATCAAGAG ATTGGGGGAG TATCGAACTA ACTTAGAGGC TGAGAGATGC 
GCAAGCACCA TCAAAACTGT CATTGAAAAT GCATTGGACA CCGAACGCAA TCAGATTGTT 
GAGCTCATAG AGATTGTGGC CAGGAAAATG GCACCGCCAA TTAGACGATA CTTGGCCGAA 
GGTGCTGAGG TGCTCGCCAA GGACTCCAAC TCCATGGACC AGTTAATGAT GTACCTGGAA 
TCCTCATTGG CCACACTCTA CGATACTCTC AATGAGATTA ATTTCCAGAG GATACTGGAT 
GGCATTTGGT CAGAGTTATC CATTATAATG TATGATCTAA TTCAATCAAA TCTTGACAAA 
CGCCGACCAC CAGCTTTCTT CCAAAATTTG AACAATACTC TTCAGACAAT GATGGACTGC 
TTTAAAATGG GCAACCTTCA AACTTCAGAC ATAAAAATAT TGTCTTCAAT TCAAAGTCGA 
CTAAGGCTTT ATTCCCTGGA GACATCGGAT CTTATTCATC AGTATTACTT GGAGCGTCTA 
GAAAACCAAA AGAGCCAAGA GTCATCTCCA TATGGACAAC TTACCATCAC GGCTCAGTTA 
ACTGATACCG GCTTATTGTT AAATATCTTA AATGCTCGCA ATCTGCTGCC AATGGATTCA 
AATGGTTCGG TGGACTCATT TGTTAAGGCC AGTTTCATGC CCACAAGTCG ATTCAACGAC 
GTACCCACTG TGAAGACCAA CGTGCACAAC AAAAGTTGTT TTCCGCTTTA CGATCAGGAG 
TTTCGTATAA ACTTGAGTGA CCACCAGCGA AGTGAAAAGA ATAGTCTTAT TGTATTTAGC 
ATCAAAGATA AAGATTTATT CGGCATGTCG AGCCAGTATA TAGCAGAGAG CTATATATCT 
TTCGCAGATC TGGAAGCGAC ACCACCGGGC GAGCAAATAA TGATGAATCT ATCCAGACCA 
GAGTATACAG ATTCCGAATC ACTGCGTGCC TTGGAATACA GACTAGGTGA TAAGCAGGCC 
AAGGATTTCC TTAAAAAGCT GAAGAACCGA TCCTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651329.4
CDS	82..3543
Translation	MDEEQMWKGFFFKLQELKMNPPTEHEHQLQDGDDGFFEKFGSLLRQKSQIDEGVLKSSLAPLEENGSGGEEDSDESPDGTLQLSQDSECTSIDTFATESTLASSSDKDISTSVLDSAPVMNVTDLYEEILFEIFNNIGCENNEECTNSLVEFVQDAFKIPNATHEEIYEAARLKEPPNVRLNVEIIKAENLMSKDSNGLSDPFVTLYLESNGSHRYNSSVKPATLNPIWEEHFSLPITENARDEVLIVEVWDFDAAETVKEKVNKILDVKGVKGLSKLMKEIAVTASSGKHDNELIGRAAITLKSIPVSGLTVWYNLEKGSKGRSRGSLLVNLALSAEKNKSVAVQEHKNLLKLLLMYELETSQVANYWWSGKFSPNAELIRSQHAAQSGLTPFDCALSQWHAYSTIHETHKLNFTLFNSILDVVVPVITYMQNDSEDVKTFWDGVKRLLPSCFAVLRKLRSKNTSDKNIIRALNEVLDILKKIKELEVPESVDIFPKSVYGWLHTNDTDETCNIDTAIEDAINTGTREWLEHIVEGSRQSKNTETDDEKLQYVIKLIQMVRSDLQRAMEYFDKIFYHKIQLNYSAVLYLFYDSKLAEICKSIIIEVCNNIKRLDVPDDQFEYLPNLENVNMGTTLFEVYLILKRYVQLGESLCSEPLELSNFYPWFERGVTHWLDISIIKALSRIQKAIDLDQLKAVDETVKYSSSAVDTLSIFYQIKIFWQQLDWPEVEGSYIFVAKIVNDLCRCCIFYAQQMSRRVENIFIADDNNKNFILSEEWCIAINNMDYIRQSLPSFIKELSIDDIIKRLGEYRTNLEAERCASTIKTVIENALDTERNQIVELIEIVARKMAPPIRRYLAEGAEVLAKDSNSMDQLMMYLESSLATLYDTLNEINFQRILDGIWSELSIIMYDLIQSNLDKRRPPAFFQNLNNTLQTMMDCFKMGNLQTSDIKILSSIQSRLRLYSLETSDLIHQYYLERLENQKSQESSPYGQLTITAQLTDTGLLLNILNARNLLPMDSNGSVDSFVKASFMPTSRFNDVPTVKTNVHNKSCFPLYDQEFRINLSDHQRSEKNSLIVFSIKDKDLFGMSSQYIAESYISFADLEATPPGEQIMMNLSRPEYTDSESLRALEYRLGDKQAKDFLKKLKNRSFS

Target ORF information:

RefSeq Version	NM_143072.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster staccato (stac), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143072.4

ORF Insert Sequence:

ATGGACGAGG AGCAGATGTG GAAGGGCTTC TTCTTCAAGT TGCAGGAGCT CAAAATGAAT 
CCACCCACGG AGCATGAACA CCAACTGCAA GACGGAGATG ATGGATTTTT CGAAAAGTTT 
GGATCCCTAC TGCGGCAGAA GTCCCAAATC GATGAGGGCG TCCTCAAAAG TTCACTGGCT 
CCCTTGGAGG AAAATGGCAG CGGCGGAGAA GAAGATTCGG ACGAGAGTCC CGATGGCACT 
TTGCAACTCA GCCAGGATTC GGAGTGCACC AGCATCGATA CCTTTGCCAC CGAGAGTACA 
CTGGCGTCCA GTTCGGACAA GGATATCAGC ACTTCAGTTC TGGACTCGGC GCCAGTGATG 
AATGTCACAG ATCTCTATGA AGAGATATTA TTTGAAATTT TCAACAACAT TGGCTGTGAA 
AACAACGAAG AGTGCACAAA CAGTTTGGTC GAATTCGTGC AGGATGCATT CAAAATTCCG 
AACGCCACTC ATGAGGAAAT TTATGAGGCG GCGCGACTGA AAGAACCGCC CAATGTTAGA 
CTAAATGTCG AGATTATAAA GGCCGAGAAC CTCATGTCCA AGGACTCAAA CGGCTTGTCC 
GATCCGTTTG TTACCCTGTA CTTGGAATCG AATGGCTCCC ATCGATACAA CTCATCGGTC 
AAGCCTGCCA CATTGAATCC CATATGGGAG GAGCACTTTT CACTGCCAAT TACTGAAAAT 
GCTCGAGACG AAGTTCTTAT CGTTGAAGTG TGGGACTTTG ATGCTGCGGA AACCGTCAAG 
GAAAAAGTCA ACAAAATCCT GGATGTTAAG GGTGTGAAAG GACTTAGCAA GCTGATGAAG 
GAAATTGCTG TAACAGCTTC CTCGGGAAAA CATGATAATG AACTGATTGG GAGAGCTGCA 
ATTACATTGA AGTCTATTCC CGTTTCCGGC CTCACCGTAT GGTATAACCT GGAAAAGGGA 
AGCAAGGGAC GCAGTCGAGG ATCACTTCTG GTTAATCTGG CCTTGAGTGC GGAGAAGAAC 
AAAAGTGTCG CCGTTCAGGA GCACAAGAAC CTACTGAAAC TGCTCCTGAT GTACGAGCTG 
GAAACCTCGC AGGTGGCCAA CTACTGGTGG AGCGGCAAGT TCAGTCCCAA TGCCGAACTC 
ATTCGATCCC AGCATGCTGC CCAGAGTGGG CTAACTCCTT TCGACTGTGC GCTCAGCCAG 
TGGCACGCGT ACAGCACCAT CCATGAGACG CACAAACTCA ATTTCACACT ATTCAATTCA 
ATTCTGGACG TGGTGGTGCC AGTGATTACT TACATGCAGA ACGACTCCGA GGATGTGAAA 
ACATTCTGGG ATGGCGTGAA ACGACTGCTT CCCTCGTGCT TCGCAGTGCT AAGGAAACTA 
AGATCCAAAA ACACCAGTGA TAAAAACATA ATTAGAGCTC TGAACGAAGT TCTGGATATT 
CTAAAGAAGA TTAAGGAGCT GGAAGTGCCG GAGAGTGTAG ACATATTCCC CAAATCGGTG 
TATGGTTGGC TACACACAAA CGACACTGAT GAAACGTGCA ATATTGATAC TGCCATTGAG 
GATGCTATCA ACACGGGGAC AAGGGAATGG CTGGAGCACA TAGTGGAGGG CAGCAGGCAA 
TCTAAGAACA CCGAAACCGA TGACGAAAAG CTGCAGTACG TCATTAAACT GATACAAATG 
GTGCGGTCTG ACCTGCAACG TGCAATGGAA TACTTCGATA AAATCTTTTA CCACAAAATT 
CAGCTTAACT ATTCCGCTGT ACTTTATTTA TTTTATGACT CCAAGCTTGC TGAAATCTGT 
AAATCGATTA TAATAGAAGT GTGCAATAAT ATTAAGAGAT TGGATGTGCC AGACGATCAA 
TTCGAATATT TACCCAACCT TGAGAATGTA AATATGGGCA CGACTTTGTT TGAAGTCTAT 
TTAATACTCA AACGTTACGT TCAATTAGGA GAATCTCTTT GCTCCGAGCC CCTGGAACTG 
TCCAACTTTT ACCCGTGGTT CGAGAGGGGA GTTACCCACT GGCTGGATAT TTCAATAATT 
AAGGCATTGA GTCGCATCCA GAAAGCAATT GATTTGGATC AGCTGAAAGC GGTCGATGAA 
ACAGTGAAAT ACAGCTCCTC GGCAGTGGAC ACACTTTCCA TATTCTACCA AATCAAGATA 
TTTTGGCAAC AACTTGACTG GCCAGAGGTC GAGGGTTCCT ACATCTTCGT AGCCAAAATC 
GTCAATGATC TTTGTCGCTG CTGCATATTT TATGCCCAAC AGATGTCCCG CAGAGTTGAG 
AATATTTTTA TTGCTGATGA TAACAATAAA AATTTCATTT TGTCGGAGGA GTGGTGCATA 
GCGATAAACA ACATGGATTA CATCCGGCAG AGCTTACCTT CATTCATAAA GGAACTTAGC 
ATTGATGACA TTATCAAGAG ATTGGGGGAG TATCGAACTA ACTTAGAGGC TGAGAGATGC 
GCAAGCACCA TCAAAACTGT CATTGAAAAT GCATTGGACA CCGAACGCAA TCAGATTGTT 
GAGCTCATAG AGATTGTGGC CAGGAAAATG GCACCGCCAA TTAGACGATA CTTGGCCGAA 
GGTGCTGAGG TGCTCGCCAA GGACTCCAAC TCCATGGACC AGTTAATGAT GTACCTGGAA 
TCCTCATTGG CCACACTCTA CGATACTCTC AATGAGATTA ATTTCCAGAG GATACTGGAT 
GGCATTTGGT CAGAGTTATC CATTATAATG TATGATCTAA TTCAATCAAA TCTTGACAAA 
CGCCGACCAC CAGCTTTCTT CCAAAATTTG AACAATACTC TTCAGACAAT GATGGACTGC 
TTTAAAATGG GCAACCTTCA AACTTCAGAC ATAAAAATAT TGTCTTCAAT TCAAAGTCGA 
CTAAGGCTTT ATTCCCTGGA GACATCGGAT CTTATTCATC AGTATTACTT GGAGCGTCTA 
GAAAACCAAA AGAGCCAAGA GTCATCTCCA TATGGACAAC TTACCATCAC GGCTCAGTTA 
ACTGATACCG GCTTATTGTT AAATATCTTA AATGCTCGCA ATCTGCTGCC AATGGATTCA 
AATGGTTCGG TGGACTCATT TGTTAAGGCC AGTTTCATGC CCACAAGTCG ATTCAACGAC 
GTACCCACTG TGAAGACCAA CGTGCACAAC AAAAGTTGTT TTCCGCTTTA CGATCAGGAG 
TTTCGTATAA ACTTGAGTGA CCACCAGCGA AGTGAAAAGA ATAGTCTTAT TGTATTTAGC 
ATCAAAGATA AAGATTTATT CGGCATGTCG AGCCAGTATA TAGCAGAGAG CTATATATCT 
TTCGCAGATC TGGAAGCGAC ACCACCGGGC GAGCAAATAA TGATGAATCT ATCCAGACCA 
GAGTATACAG ATTCCGAATC ACTGCGTGCC TTGGAATACA GACTAGGTGA TAAGCAGGCC 
AAGGATTTCC TTAAAAAGCT GAAGAACCGA TCCTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stac ( NM_001104439.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097909.2 Drosophila melanogaster staccato (stac), transcript variant D, mRNA.

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CloneID	OFa08797
Clone ID Related Accession (Same CDS sequence)	NM_001104439.3
Accession Version	NM_001104439.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	staccato, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104439.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTACC TATCGCTACC ACGTGTTCTG AGCAAGTTCG GCTCCCAAAA CCACAGTTCG 
TCTAACCAAA GTTCGAAAAC CAATAGCCTA ACTAGATCCA GTACGCGCAC CGGATGCGAC 
GAAATCAGAC TGCCGCGGAA GGCATCCTCC ACCCAGTCGC TCAACAAACC ATTCGAGCGA 
CGGATGAGCA TACTGAATCG GAATGGAAGC ATTTCCAGTC GACACTTCCG TGGTGTCCAC 
AAGCATTTAC TTGATTCCCA TGCCAAGGCG GTCTGCCACT CGATGACCGT CACAGATCTC 
TATGAAGAGA TATTATTTGA AATTTTCAAC AACATTGGCT GTGAAAACAA CGAAGAGTGC 
ACAAACAGTT TGGTCGAATT CGTGCAGGAT GCATTCAAAA TTCCGAACGC CACTCATGAG 
GAAATTTATG AGGCGGCGCG ACTGAAAGAA CCGCCCAATG TTAGACTAAA TGTCGAGATT 
ATAAAGGCCG AGAACCTCAT GTCCAAGGAC TCAAACGGCT TGTCCGATCC GTTTGTTACC 
CTGTACTTGG AATCGAATGG CTCCCATCGA TACAACTCAT CGGTCAAGCC TGCCACATTG 
AATCCCATAT GGGAGGAGCA CTTTTCACTG CCAATTACTG AAAATGCTCG AGACGAAGTT 
CTTATCGTTG AAGTGTGGGA CTTTGATGCT GCGGAAACCG TCAAGGAAAA AGTCAACAAA 
ATCCTGGATG TTAAGGGTGT GAAAGGACTT AGCAAGCTGA TGAAGGAAAT TGCTGTAACA 
GCTTCCTCGG GAAAACATGA TAATGAACTG ATTGGGAGAG CTGCAATTAC ATTGAAGTCT 
ATTCCCGTTT CCGGCCTCAC CGTATGGTAT AACCTGGAAA AGGGAAGCAA GGGACGCAGT 
CGAGGATCAC TTCTGGTTAA TCTGGCCTTG AGTGCGGAGA AGAACAAAAG TGTCGCCGTT 
CAGGAGCACA AGAACCTACT GAAACTGCTC CTGATGTACG AGCTGGAAAC CTCGCAGGTG 
GCCAACTACT GGTGGAGCGG CAAGTTCAGT CCCAATGCCG AACTCATTCG ATCCCAGCAT 
GCTGCCCAGA GTGGGCTAAC TCCTTTCGAC TGTGCGCTCA GCCAGTGGCA CGCGTACAGC 
ACCATCCATG AGACGCACAA ACTCAATTTC ACACTATTCA ATTCAATTCT GGACGTGGTG 
GTGCCAGTGA TTACTTACAT GCAGAACGAC TCCGAGGATG TGAAAACATT CTGGGATGGC 
GTGAAACGAC TGCTTCCCTC GTGCTTCGCA GTGCTAAGGA AACTAAGATC CAAAAACACC 
AGTGATAAAA ACATAATTAG AGCTCTGAAC GAAGTTCTGG ATATTCTAAA GAAGATTAAG 
GAGCTGGAAG TGCCGGAGAG TGTAGACATA TTCCCCAAAT CGGTGTATGG TTGGCTACAC 
ACAAACGACA CTGATGAAAC GTGCAATATT GATACTGCCA TTGAGGATGC TATCAACACG 
GGGACAAGGG AATGGCTGGA GCACATAGTG GAGGGCAGCA GGCAATCTAA GAACACCGAA 
ACCGATGACG AAAAGCTGCA GTACGTCATT AAACTGATAC AAATGGTGCG GTCTGACCTG 
CAACGTGCAA TGGAATACTT CGATAAAATC TTTTACCACA AAATTCAGCT TAACTATTCC 
GCTGTACTTT ATTTATTTTA TGACTCCAAG CTTGCTGAAA TCTGTAAATC GATTATAATA 
GAAGTGTGCA ATAATATTAA GAGATTGGAT GTGCCAGACG ATCAATTCGA ATATTTACCC 
AACCTTGAGA ATGTAAATAT GGGCACGACT TTGTTTGAAG TCTATTTAAT ACTCAAACGT 
TACGTTCAAT TAGGAGAATC TCTTTGCTCC GAGCCCCTGG AACTGTCCAA CTTTTACCCG 
TGGTTCGAGA GGGGAGTTAC CCACTGGCTG GATATTTCAA TAATTAAGGC ATTGAGTCGC 
ATCCAGAAAG CAATTGATTT GGATCAGCTG AAAGCGGTCG ATGAAACAGT GAAATACAGC 
TCCTCGGCAG TGGACACACT TTCCATATTC TACCAAATCA AGATATTTTG GCAACAACTT 
GACTGGCCAG AGGTCGAGGG TTCCTACATC TTCGTAGCCA AAATCGTCAA TGATCTTTGT 
CGCTGCTGCA TATTTTATGC CCAACAGATG TCCCGCAGAG TTGAGAATAT TTTTATTGCT 
GATGATAACA ATAAAAATTT CATTTTGTCG GAGGAGTGGT GCATAGCGAT AAACAACATG 
GATTACATCC GGCAGAGCTT ACCTTCATTC ATAAAGGAAC TTAGCATTGA TGACATTATC 
AAGAGATTGG GGGAGTATCG AACTAACTTA GAGGCTGAGA GATGCGCAAG CACCATCAAA 
ACTGTCATTG AAAATGCATT GGACACCGAA CGCAATCAGA TTGTTGAGCT CATAGAGATT 
GTGGCCAGGA AAATGGCACC GCCAATTAGA CGATACTTGG CCGAAGGTGC TGAGGTGCTC 
GCCAAGGACT CCAACTCCAT GGACCAGTTA ATGATGTACC TGGAATCCTC ATTGGCCACA 
CTCTACGATA CTCTCAATGA GATTAATTTC CAGAGGATAC TGGATGGCAT TTGGTCAGAG 
TTATCCATTA TAATGTATGA TCTAATTCAA TCAAATCTTG ACAAACGCCG ACCACCAGCT 
TTCTTCCAAA ATTTGAACAA TACTCTTCAG ACAATGATGG ACTGCTTTAA AATGGGCAAC 
CTTCAAACTT CAGACATAAA AATATTGTCT TCAATTCAAA GTCGACTAAG GCTTTATTCC 
CTGGAGACAT CGGATCTTAT TCATCAGTAT TACTTGGAGC GTCTAGAAAA CCAAAAGAGC 
CAAGAGTCAT CTCCATATGG ACAACTTACC ATCACGGCTC AGTTAACTGA TACCGGCTTA 
TTGTTAAATA TCTTAAATGC TCGCAATCTG CTGCCAATGG ATTCAAATGG TTCGGTGGAC 
TCATTTGTTA AGGCCAGTTT CATGCCCACA AGTCGATTCA ACGACGTACC CACTGTGAAG 
ACCAACGTGC ACAACAAAAG TTGTTTTCCG CTTTACGATC AGGAGTTTCG TATAAACTTG 
AGTGACCACC AGCGAAGTGA AAAGAATAGT CTTATTGTAT TTAGCATCAA AGATAAAGAT 
TTATTCGGCA TGTCGAGCCA GTATATAGCA GAGAGCTATA TATCTTTCGC AGATCTGGAA 
GCGACACCAC CGGGCGAGCA AATAATGATG AATCTATCCA GACCAGAGTA TACAGATTCC 
GAATCACTGC GTGCCTTGGA ATACAGACTA GGTGATAAGC AGGCCAAGGA TTTCCTTAAA 
AAGCTGAAGA ACCGATCCTT CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097909.2
CDS	190..3576
Translation	MSYLSLPRVLSKFGSQNHSSSNQSSKTNSLTRSSTRTGCDEIRLPRKASSTQSLNKPFERRMSILNRNGSISSRHFRGVHKHLLDSHAKAVCHSMTVTDLYEEILFEIFNNIGCENNEECTNSLVEFVQDAFKIPNATHEEIYEAARLKEPPNVRLNVEIIKAENLMSKDSNGLSDPFVTLYLESNGSHRYNSSVKPATLNPIWEEHFSLPITENARDEVLIVEVWDFDAAETVKEKVNKILDVKGVKGLSKLMKEIAVTASSGKHDNELIGRAAITLKSIPVSGLTVWYNLEKGSKGRSRGSLLVNLALSAEKNKSVAVQEHKNLLKLLLMYELETSQVANYWWSGKFSPNAELIRSQHAAQSGLTPFDCALSQWHAYSTIHETHKLNFTLFNSILDVVVPVITYMQNDSEDVKTFWDGVKRLLPSCFAVLRKLRSKNTSDKNIIRALNEVLDILKKIKELEVPESVDIFPKSVYGWLHTNDTDETCNIDTAIEDAINTGTREWLEHIVEGSRQSKNTETDDEKLQYVIKLIQMVRSDLQRAMEYFDKIFYHKIQLNYSAVLYLFYDSKLAEICKSIIIEVCNNIKRLDVPDDQFEYLPNLENVNMGTTLFEVYLILKRYVQLGESLCSEPLELSNFYPWFERGVTHWLDISIIKALSRIQKAIDLDQLKAVDETVKYSSSAVDTLSIFYQIKIFWQQLDWPEVEGSYIFVAKIVNDLCRCCIFYAQQMSRRVENIFIADDNNKNFILSEEWCIAINNMDYIRQSLPSFIKELSIDDIIKRLGEYRTNLEAERCASTIKTVIENALDTERNQIVELIEIVARKMAPPIRRYLAEGAEVLAKDSNSMDQLMMYLESSLATLYDTLNEINFQRILDGIWSELSIIMYDLIQSNLDKRRPPAFFQNLNNTLQTMMDCFKMGNLQTSDIKILSSIQSRLRLYSLETSDLIHQYYLERLENQKSQESSPYGQLTITAQLTDTGLLLNILNARNLLPMDSNGSVDSFVKASFMPTSRFNDVPTVKTNVHNKSCFPLYDQEFRINLSDHQRSEKNSLIVFSIKDKDLFGMSSQYIAESYISFADLEATPPGEQIMMNLSRPEYTDSESLRALEYRLGDKQAKDFLKKLKNRSFS

Target ORF information:

RefSeq Version	NM_001104439.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster staccato (stac), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104439.3

ORF Insert Sequence:

ATGAGCTACC TATCGCTACC ACGTGTTCTG AGCAAGTTCG GCTCCCAAAA CCACAGTTCG 
TCTAACCAAA GTTCGAAAAC CAATAGCCTA ACTAGATCCA GTACGCGCAC CGGATGCGAC 
GAAATCAGAC TGCCGCGGAA GGCATCCTCC ACCCAGTCGC TCAACAAACC ATTCGAGCGA 
CGGATGAGCA TACTGAATCG GAATGGAAGC ATTTCCAGTC GACACTTCCG TGGTGTCCAC 
AAGCATTTAC TTGATTCCCA TGCCAAGGCG GTCTGCCACT CGATGACCGT CACAGATCTC 
TATGAAGAGA TATTATTTGA AATTTTCAAC AACATTGGCT GTGAAAACAA CGAAGAGTGC 
ACAAACAGTT TGGTCGAATT CGTGCAGGAT GCATTCAAAA TTCCGAACGC CACTCATGAG 
GAAATTTATG AGGCGGCGCG ACTGAAAGAA CCGCCCAATG TTAGACTAAA TGTCGAGATT 
ATAAAGGCCG AGAACCTCAT GTCCAAGGAC TCAAACGGCT TGTCCGATCC GTTTGTTACC 
CTGTACTTGG AATCGAATGG CTCCCATCGA TACAACTCAT CGGTCAAGCC TGCCACATTG 
AATCCCATAT GGGAGGAGCA CTTTTCACTG CCAATTACTG AAAATGCTCG AGACGAAGTT 
CTTATCGTTG AAGTGTGGGA CTTTGATGCT GCGGAAACCG TCAAGGAAAA AGTCAACAAA 
ATCCTGGATG TTAAGGGTGT GAAAGGACTT AGCAAGCTGA TGAAGGAAAT TGCTGTAACA 
GCTTCCTCGG GAAAACATGA TAATGAACTG ATTGGGAGAG CTGCAATTAC ATTGAAGTCT 
ATTCCCGTTT CCGGCCTCAC CGTATGGTAT AACCTGGAAA AGGGAAGCAA GGGACGCAGT 
CGAGGATCAC TTCTGGTTAA TCTGGCCTTG AGTGCGGAGA AGAACAAAAG TGTCGCCGTT 
CAGGAGCACA AGAACCTACT GAAACTGCTC CTGATGTACG AGCTGGAAAC CTCGCAGGTG 
GCCAACTACT GGTGGAGCGG CAAGTTCAGT CCCAATGCCG AACTCATTCG ATCCCAGCAT 
GCTGCCCAGA GTGGGCTAAC TCCTTTCGAC TGTGCGCTCA GCCAGTGGCA CGCGTACAGC 
ACCATCCATG AGACGCACAA ACTCAATTTC ACACTATTCA ATTCAATTCT GGACGTGGTG 
GTGCCAGTGA TTACTTACAT GCAGAACGAC TCCGAGGATG TGAAAACATT CTGGGATGGC 
GTGAAACGAC TGCTTCCCTC GTGCTTCGCA GTGCTAAGGA AACTAAGATC CAAAAACACC 
AGTGATAAAA ACATAATTAG AGCTCTGAAC GAAGTTCTGG ATATTCTAAA GAAGATTAAG 
GAGCTGGAAG TGCCGGAGAG TGTAGACATA TTCCCCAAAT CGGTGTATGG TTGGCTACAC 
ACAAACGACA CTGATGAAAC GTGCAATATT GATACTGCCA TTGAGGATGC TATCAACACG 
GGGACAAGGG AATGGCTGGA GCACATAGTG GAGGGCAGCA GGCAATCTAA GAACACCGAA 
ACCGATGACG AAAAGCTGCA GTACGTCATT AAACTGATAC AAATGGTGCG GTCTGACCTG 
CAACGTGCAA TGGAATACTT CGATAAAATC TTTTACCACA AAATTCAGCT TAACTATTCC 
GCTGTACTTT ATTTATTTTA TGACTCCAAG CTTGCTGAAA TCTGTAAATC GATTATAATA 
GAAGTGTGCA ATAATATTAA GAGATTGGAT GTGCCAGACG ATCAATTCGA ATATTTACCC 
AACCTTGAGA ATGTAAATAT GGGCACGACT TTGTTTGAAG TCTATTTAAT ACTCAAACGT 
TACGTTCAAT TAGGAGAATC TCTTTGCTCC GAGCCCCTGG AACTGTCCAA CTTTTACCCG 
TGGTTCGAGA GGGGAGTTAC CCACTGGCTG GATATTTCAA TAATTAAGGC ATTGAGTCGC 
ATCCAGAAAG CAATTGATTT GGATCAGCTG AAAGCGGTCG ATGAAACAGT GAAATACAGC 
TCCTCGGCAG TGGACACACT TTCCATATTC TACCAAATCA AGATATTTTG GCAACAACTT 
GACTGGCCAG AGGTCGAGGG TTCCTACATC TTCGTAGCCA AAATCGTCAA TGATCTTTGT 
CGCTGCTGCA TATTTTATGC CCAACAGATG TCCCGCAGAG TTGAGAATAT TTTTATTGCT 
GATGATAACA ATAAAAATTT CATTTTGTCG GAGGAGTGGT GCATAGCGAT AAACAACATG 
GATTACATCC GGCAGAGCTT ACCTTCATTC ATAAAGGAAC TTAGCATTGA TGACATTATC 
AAGAGATTGG GGGAGTATCG AACTAACTTA GAGGCTGAGA GATGCGCAAG CACCATCAAA 
ACTGTCATTG AAAATGCATT GGACACCGAA CGCAATCAGA TTGTTGAGCT CATAGAGATT 
GTGGCCAGGA AAATGGCACC GCCAATTAGA CGATACTTGG CCGAAGGTGC TGAGGTGCTC 
GCCAAGGACT CCAACTCCAT GGACCAGTTA ATGATGTACC TGGAATCCTC ATTGGCCACA 
CTCTACGATA CTCTCAATGA GATTAATTTC CAGAGGATAC TGGATGGCAT TTGGTCAGAG 
TTATCCATTA TAATGTATGA TCTAATTCAA TCAAATCTTG ACAAACGCCG ACCACCAGCT 
TTCTTCCAAA ATTTGAACAA TACTCTTCAG ACAATGATGG ACTGCTTTAA AATGGGCAAC 
CTTCAAACTT CAGACATAAA AATATTGTCT TCAATTCAAA GTCGACTAAG GCTTTATTCC 
CTGGAGACAT CGGATCTTAT TCATCAGTAT TACTTGGAGC GTCTAGAAAA CCAAAAGAGC 
CAAGAGTCAT CTCCATATGG ACAACTTACC ATCACGGCTC AGTTAACTGA TACCGGCTTA 
TTGTTAAATA TCTTAAATGC TCGCAATCTG CTGCCAATGG ATTCAAATGG TTCGGTGGAC 
TCATTTGTTA AGGCCAGTTT CATGCCCACA AGTCGATTCA ACGACGTACC CACTGTGAAG 
ACCAACGTGC ACAACAAAAG TTGTTTTCCG CTTTACGATC AGGAGTTTCG TATAAACTTG 
AGTGACCACC AGCGAAGTGA AAAGAATAGT CTTATTGTAT TTAGCATCAA AGATAAAGAT 
TTATTCGGCA TGTCGAGCCA GTATATAGCA GAGAGCTATA TATCTTTCGC AGATCTGGAA 
GCGACACCAC CGGGCGAGCA AATAATGATG AATCTATCCA GACCAGAGTA TACAGATTCC 
GAATCACTGC GTGCCTTGGA ATACAGACTA GGTGATAAGC AGGCCAAGGA TTTCCTTAAA 
AAGCTGAAGA ACCGATCCTT CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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stac cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

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