asp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	asp
Entrez Gene ID	42946
Full Name	abnormal spindle
Synonyms	ASP,Asp,CG6875,Dm Asp,Dmel\CG6875,anon-96Aa,anon-WO0118547.279
General protein information	Preferred Names abnormal spindle Names CG6875 gene product from transcript CG6875-RA CG6875-PA abnormal spindle protein abnormal spindles asp-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96A19-96A20\|3-85.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079764.3	NP_524488.3	abnormal spindle

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	asp	NP_524488.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003703	XP_313496.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in this study we have provided evidence that ASPM controls spindle orientation by regulating the dynamics of astral MT and that CITK is a critical downstream partner of ASPM for this activity.
Title: ASPM and CITK regulate spindle orientation by affecting the dynamics of astral microtubules.

Asp at the minus ends of the microtubules cross-links MTs at the pole and within the spindle
Title: Microcephaly protein Asp focuses the minus ends of spindle microtubules at the pole and within the spindle.

Asp-CaM complex has a critical role in centrosome-pole cohesion and centrosome inheritance in neural stem cells
Title: An Asp-CaM complex is required for centrosome-pole cohesion and centrosome inheritance in neural stem cells.

propose that Asp regulates neuroepithelium morphogenesis through myosin-II-mediated structural and mechanical processes to maintain force balance and tissue cohesiveness
Title: The microcephaly protein Asp regulates neuroepithelium morphogenesis by?controlling the?spatial distribution of myosin II.

dynein, abnormal spindle protein (Asp), and KLP10A have roles in Drosophila spindle pole organization
Title: Spindle pole organization in Drosophila S2 cells by dynein, abnormal spindle protein (Asp), and KLP10A.

The following asp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the asp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06008	NM_079764.3 Latest version!	Drosophila melanogaster abnormal spindle (asp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

asp ( NM_079764.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524488.3 Drosophila melanogaster abnormal spindle (asp), mRNA.

Price & Availability↑

CloneID	OFa06008
Clone ID Related Accession (Same CDS sequence)	NM_079764.3
Accession Version	NM_079764.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	abnormal spindle
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_079764.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGCCT TTGAGATCAC AGTGACGCCA TCGCGCCTAA AGCAAAAGAA GCGCGCCGAA 
GGACGCGAGC CCGCCGTCGT CGTCATGGCT CCGTTCTCCG CCAAGGCGAT TGTGCAGTTC 
GAGGATGTGC CGATAACAAA GACAGCACGG CGCCAGGTGC GCGTTCTCAA TCCCAGCGAC 
GACGACATCG AGGTCAAGGT AATGAAGGCC ATCCGGGAGG AGCACAACTT AAGCCTGGAG 
TGGATGGAGC ACACGGTGCC TGCAAGAGAT GAGGTATCCA TGGAGCTAGT GTGGAGCCCC 
GTCCTTGAGG TGGCCTGCAA GGAGACGCTG CAGCTAATAG ACAACCGCAA CTTCCGAAAG 
GAGGTGATGA TCATACTCAA GTCCAAGAGC AACCAGCCGG TCAAGAACCC GCGCAAATTT 
CCTACTGTCG GCAAGACCCT GCAGCTGAAA TCGCCGACAG GAGCTGGCAA GACAATGAAA 
AGCGTGGTAT CCGCTGCTGT GCAGCAAAAG AAGCGCATGT CTGCAGCAGC AGCGCCTCCC 
TCCAAGCAGA CATGGCGAGT GACTGCTCCT TCGCGTCCCG CTGCATGGGC ACATCCACCT 
CCACAGGCTC CTCTTGTCGA GAAGAATGTA TACAAGACTC CACAAGAAGA GCCCGTATAC 
ATATCACCAC AGCCTCGCAG TCTTAAGGAA AATCTAAGCC CTATGACGCC AGGAAACCTA 
CTCGACGTGA TTGACAATCT GCGATTCACA CCTCTCACCG AAACCCGTGG CAAAGGACAA 
GCTACCATTT TCCCGGACAA TCTGGCAGCC TGGCCCACAC CAACACTTAA AGGGAATGTA 
AAATCATGTG CTAATGATAT GCGGCCGCGT CGAATCACTC CAGATGATCT AGAAGATCAG 
CCTGCCACAA ACAAAACGTT CGATGTAAAG CATTCCGAGA CTATCAATAT TTCGTTGGAC 
ACCTTGGACT GCTCCAGGAT CGATGGACAA CCGCATACGC CCCTAAATAA GACAACAACC 
ATTGTGCATG CCACGCACAC CAGAGCTCTG GCCTGTATTC ATGAGGAGGA GGGACCAAGT 
CCACCTAGGA CGCCCACGAA GAGCGCCATA CACGACCTGA AGAGGGACAT TAAGTTGGTG 
GGTTCACCCT TACGAAAGTA TTCCGAGTCC ATGAAAGATT TGTCACTTCT ATCGCCACAA 
ACTAAGTATG CCATTCAAGG GTCTATGCCT AATCTAAATG AAATGAAAAT CCGCTCGATC 
GAACAGAATA GATATTACCA GGAGCAGCAG ATCCAGATAA AGGCCAAAGA CTTGAATAGC 
TCCTCTAGTA GCGAGGCTAG TTTGGCCGGC CAGCAGGAGT TTCTATTCAA CCACAGTGAG 
ATCCTCGCTC AGTCCAGTCG TTTTAATCTC CATGAAGTAG GTCGGAAGTC GGTGAAGGGA 
AGTCCGGTGA AGAATCCTCA CAAGCGCCGC TCTCATGAGT TGAGTTTTTC GGATGCACCT 
AGCAACGAAT CATTGTACCG CAATGAAACT GTAGCCATTT CCCCTCCTAA AAAGCAACGG 
GTTGAGGACA CTACTCTGCC CAGAAGTGCA GCGCCGGCAA ATGCATCTGC AAGAAGCAGT 
AGTGCCCACG CCTGGCCACA CGCCCAATCC AAGAAGTTTA AGCTAGCACA AACCATGTCA 
CTGATGAAGA AGCCCGCCAC ACCACGAAAA GTCAGGGACA CTAGCATTCA GCCTTCCGTC 
AAGCTCTATG ACTCGGAGCT GTATATGCAG ACGTGCATCA ACCCGGATCC ATTTGCAGCA 
ACTACGACAA TTGATCCATT TCTGGCATCT ACCATGTATT TGGATGAACA GGCTGTGGAT 
CGTCATCAAG CTGACTTTAA AAAGTGGTTA AATGCCCTTG TCTCCATACC CGCTGACCTG 
GACGCAGATT TAAATAACAA AATAGACGTT GGTAAGCTGT TTAACGAGGT GCGCAACAAA 
GAGCTCGTGG TGGCTCCCAC CAAGGAGGAG CAGTCTATGA ACTACCTAAC GAAATACCGC 
CTGGAGACGC TTCGTAAGGC GGCTGTGGAG CTCTTCTTCA GTGAGCAGAT GCGCCTGCCA 
TGCTCCAAAG TGGCCGTATA TGTCAACAAG CAAGCTCTGC GCATCCGTAG CGATCGTAAT 
CTTCACCTAG ACGTAGTTAT GCAACGCACC ATACTTGAGC TGCTGCTTTG CTTCAATCCC 
CTTTGGCTGC GCCTTGGACT GGAAGTGGTC TTTGGCGAGA AGATCCAGAT GCAGTCTAAT 
CGAGACATTG TAGGCCTCAG CACCTTTATC CTCAATCGCT TGTTCCGGAA TAAGTGTGAG 
GAGCAGAGGT ACAGCAAGGC ATACACACTC ACCGAAGAGT ACGCGGAGAC CATTAAGAAG 
CACTCATTGC AGAAAATCCT CTTTTTGCTG CTTTTCCTCG ATCAAGCTAA GCAGAAGCGC 
ATCGTCAAGC ACAATCCCTG TTTGTTTGTT AAAAAGTCGC CACATAAAGA GACCAAGGAT 
ATTCTGCTGC GCTTCTCGTC GGAGCTGCTC GCCAACATTG GTGATATTAC GCGGGAACTT 
CGTCGCCTGG GCTACGTTCT ACAGCACCGC CAAACATTTT TGGACGAGTT CGATTATGCC 
TTCAACAACT TGGCTGTGGA CCTAAGAGAT GGCGTGAGAC TAACCCGAGT CATGGAGGTA 
ATTTTACTAC GCGATGATCT AACCCGCCAG TTAAGGGTGC CAGCCATCTC TCGCCTTCAG 
CGGATCTTCA ATGTAAAGCT AGCTCTGGGC GCACTTGGTG AAGCCAACTT CCAGCTAGGC 
GGCGACATCG CCGCCCAAGA CATCGTTGAC GGACATCGTG AGAAGACGCT TTCCCTGCTC 
TGGCAACTTA TTTACAAATT CCGCTCGCCC AAGTTTCATG CGGCGGCCAC GGTGCTCCAG 
AAATGGTGGC GCCGTCACTG GCTGCACGTT GTTATCCAGC GTCGCATTCG CCACAAAGAG 
CTTATGCGTC GCCACCGGGC CGCTACTGTC ATTCAGGCCG TGTTCCGTGG CCACCAGATG 
AGAAAGTACG TGAAGTTGTT TAAGACGGAA CGCACTCAGG CCGCAATAAT TCTGCAAAAG 
TTCACCCGTC GGTATTTGGC CCAGAAGCAG CTATATCAGA GCTATCACAG TATTATCACC 
ATCCAGCGCT GGTGGCGAGC CCAACAACTG GGAAGGCAGC ACCGCCAGCG GTTTGTGGAG 
CTCCGAGAGG CTGCGATCTT TCTTCAGCGG ATCTGGCGGC GACGACTCTT TGCCAAAAAA 
CTATTGGCGG CGGCGGAAAC AGCCAGACTT CAGCGATCGC AAAAACAACA GGCAGCTGCT 
AGTTATATTC AAATGCAATG GCGAAGCTAT CAGCTGGGCA GAATTCAGCG ACAGCAGTTC 
CTGCGCCAGA GGGACCTCAT CATGTTTGTT CAGCGCAGGA TGCGAAGCAA GTGGAGTATG 
CTGGAGCAGC GCAAGGAGTT CCAGCAACTA AAGCGTGCAG CTATAAATAT CCAACAACGC 
TGGCGAGCGA AGCTTTCAAT GAGAAAGTGC AACGCTGATT ATTTGGCACT TCGTTCCAGC 
GTTCTTAAAG TTCAGGCTTA CAGGAAAGCC ACAATCCAGA TGAGAATAGA TCGTAATCAC 
TACTATTCCC TGCGAAAAAA TGTTATCTGC CTGCAACAGC GACTGAGGGC CATCATGAAA 
ATGCGCGAAC AGAGGGAAAA TTATCTGAGG CTGCGAAATG CTTCAATACT AGTTCAAAAA 
CGCTACCGCA TGCGTCAACA AATGATCCAG GATAGAAATG CATATTTAAG AACCCGCAAA 
TGTATCATCA ATGTCCAGAG GCGCTGGAGA GCCACTCTGC AGATGCGTCG AGAAAGGAAG 
AACTACCTTC ATCTCCAAAC GACAACCAAA CGAATTCAAA TCAAGTTCCG TGCCAAGCGC 
GAAATGAAAA AGCAAAGAGC CGAGTTTCTT CAGCTGAAAA AGGTTACCCT TGTAGTCCAG 
AAACGTCGGC GGGCTTTGCT GCAGATGCGA AAGGAGCGCC AGGAGTACCT ACATCTGCGC 
GAGGTTACCA TAAAGCTGCA GCGCAGATTC CATGCTCAAA AATCAATGCG GTTCATGCGA 
GCCAAGTACC GCGGCACCCA GGCTGCTGTG AGCTGCCTGC AGATGCATTG GCGTAATCAT 
CTCCTTAGGA AACGGGAGAG GAACAGTTTC TTACAACTGC GTCAAGCAGC AATAACACTG 
CAGCGACGGT ACCGAGCTCG TCTGAATATG ATCAAGCAGT TGAAGAGTTA CGCCCAGCTG 
AAACAGGCAG CTATTACCAT TCAAACCCGA TATAGAGCCA AGAAGGCAAT GCAAAAGCAG 
GTGGTCTTGT ACCAAAAGCA AAGGGAAGCC ATTATCAAAG TGCAACGACG ATACCGCGGC 
AATCTGGAGA TGAGGAAGCA GATTGAAGTC TACCAAAAAC AGCGCCAGGC AGTCATCCGC 
TTGCAGAAAT GGTGGCGCAG TATACGCGAC ATGCGGCTGT GCAAAGCGGG CTACCGAAGG 
ATTCGACTCA GTTCATTGAG CATTCAACGC AAGTGGCGGG CCACAGTGCA GGCTCGTCGC 
CAACGGGAGA TCTTCTTAAG CACCATCCGC AAAGTGCGAC TTATGCAGGC ATTTATCAGA 
GCAACTTTAC TGATGCGTCA GCAGCGCAGG GAATTCGAGA TGAAGCGAAG GGCTGCCGTA 
GTGATCCAGC GCCGGTTTCG TGCTCGCTGT GCTATGCTAA AGGCGAGACA GGATTACCAA 
TTAATCCAAT CCTCTGTGAT CCTGGTGCAG CGCAAATTCC GTGCTAATCG CAGCATGAAG 
CAGGCACGCC AGGAATTTGT CCAGCTGCGT ACTATTGCAG TCCATTTGCA ACAAAAGTTC 
CGTGGCAAGC GTCTAATGAT TGAGCAGCGT AATTGTTTCC AACTGCTCCG CTGTTCCATG 
CCGGGCTTCC AGGCACGTGC CCGCGGCTTT ATGGCTCGCA AACGATTCCA GGCCCTGATG 
ACACCCGAGA TGATGGACCT CATCCGCCAG AAGCGCGCCG CCAAGGTTAT ACAGCGTTAC 
TGGCGGGGCT ATCTAATCCG ACGACGTCAG AAGCACCAGG GTCTCTTGGA TATCCGGAAG 
CGTATTGCTC AGCTGCGGCA AGAGGCAAAG GCGGTAAACT CTGTGCGCTG CAAAGTCCAG 
GAGGCGGTGC GTTTCCTTCG CGGACGCTTT ATCGCATCAG ATGCGTTAGC AGTGCTAAGT 
CGATTGGATC GTCTTTCGCG CACTGTGCCA CACCTGCTCA TGTGGTGTTC GGAGTTCATG 
TCCACGTTTT GCTATGGCAT CATGGCTCAG GCCATTCGAT CAGAGGTGGA TAAGCAGCTT 
ATAGAGCGCT GCAGCCGGAT CATCCTAAAT TTGGCCCGCT ACAATAGCAC CACGGTGAAC 
ACGTTCCAGG AGGGCGGTTT GGTCACCATT GCCCAGATGT TATTGCGCTG GTGCGACAAA 
GACAGTGAGA TATTCAACAC TTTGTGCACC CTCATTTGGG TATTCGCCCA CTGTCCTAAA 
AAGCGAAAGA TCATTCACGA CTATATGACC AACCCAGAGG CCATTTACAT GGTGCGCGAA 
ACTAAGAAGC TCGTGGCCCG CAAGGAAAAG ATGAAGCAAA ATGCACGCAA GCCGCCGCCA 
ATGACAAGTG GACGGTATAA GAGCCAAAAG ATAAACTTCA CGCCGTGTTC CCTGCCCAGC 
CTGGAGCCGG ACTTCGGAAT CATCCGCTAC AGTCCCTACA CGTTTATCTC GTCCGTTTAC 
GCCTTCGATA CGATTTTGTG CAAGCTGCAG ATCGACATGT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524488.3
CDS	193..6057
Translation	MSAFEITVTPSRLKQKKRAEGREPAVVVMAPFSAKAIVQFEDVPITKTARRQVRVLNPSDDDIEVKVMKAIREEHNLSLEWMEHTVPARDEVSMELVWSPVLEVACKETLQLIDNRNFRKEVMIILKSKSNQPVKNPRKFPTVGKTLQLKSPTGAGKTMKSVVSAAVQQKKRMSAAAAPPSKQTWRVTAPSRPAAWAHPPPQAPLVEKNVYKTPQEEPVYISPQPRSLKENLSPMTPGNLLDVIDNLRFTPLTETRGKGQATIFPDNLAAWPTPTLKGNVKSCANDMRPRRITPDDLEDQPATNKTFDVKHSETINISLDTLDCSRIDGQPHTPLNKTTTIVHATHTRALACIHEEEGPSPPRTPTKSAIHDLKRDIKLVGSPLRKYSESMKDLSLLSPQTKYAIQGSMPNLNEMKIRSIEQNRYYQEQQIQIKAKDLNSSSSSEASLAGQQEFLFNHSEILAQSSRFNLHEVGRKSVKGSPVKNPHKRRSHELSFSDAPSNESLYRNETVAISPPKKQRVEDTTLPRSAAPANASARSSSAHAWPHAQSKKFKLAQTMSLMKKPATPRKVRDTSIQPSVKLYDSELYMQTCINPDPFAATTTIDPFLASTMYLDEQAVDRHQADFKKWLNALVSIPADLDADLNNKIDVGKLFNEVRNKELVVAPTKEEQSMNYLTKYRLETLRKAAVELFFSEQMRLPCSKVAVYVNKQALRIRSDRNLHLDVVMQRTILELLLCFNPLWLRLGLEVVFGEKIQMQSNRDIVGLSTFILNRLFRNKCEEQRYSKAYTLTEEYAETIKKHSLQKILFLLLFLDQAKQKRIVKHNPCLFVKKSPHKETKDILLRFSSELLANIGDITRELRRLGYVLQHRQTFLDEFDYAFNNLAVDLRDGVRLTRVMEVILLRDDLTRQLRVPAISRLQRIFNVKLALGALGEANFQLGGDIAAQDIVDGHREKTLSLLWQLIYKFRSPKFHAAATVLQKWWRRHWLHVVIQRRIRHKELMRRHRAATVIQAVFRGHQMRKYVKLFKTERTQAAIILQKFTRRYLAQKQLYQSYHSIITIQRWWRAQQLGRQHRQRFVELREAAIFLQRIWRRRLFAKKLLAAAETARLQRSQKQQAAASYIQMQWRSYQLGRIQRQQFLRQRDLIMFVQRRMRSKWSMLEQRKEFQQLKRAAINIQQRWRAKLSMRKCNADYLALRSSVLKVQAYRKATIQMRIDRNHYYSLRKNVICLQQRLRAIMKMREQRENYLRLRNASILVQKRYRMRQQMIQDRNAYLRTRKCIINVQRRWRATLQMRRERKNYLHLQTTTKRIQIKFRAKREMKKQRAEFLQLKKVTLVVQKRRRALLQMRKERQEYLHLREVTIKLQRRFHAQKSMRFMRAKYRGTQAAVSCLQMHWRNHLLRKRERNSFLQLRQAAITLQRRYRARLNMIKQLKSYAQLKQAAITIQTRYRAKKAMQKQVVLYQKQREAIIKVQRRYRGNLEMRKQIEVYQKQRQAVIRLQKWWRSIRDMRLCKAGYRRIRLSSLSIQRKWRATVQARRQREIFLSTIRKVRLMQAFIRATLLMRQQRREFEMKRRAAVVIQRRFRARCAMLKARQDYQLIQSSVILVQRKFRANRSMKQARQEFVQLRTIAVHLQQKFRGKRLMIEQRNCFQLLRCSMPGFQARARGFMARKRFQALMTPEMMDLIRQKRAAKVIQRYWRGYLIRRRQKHQGLLDIRKRIAQLRQEAKAVNSVRCKVQEAVRFLRGRFIASDALAVLSRLDRLSRTVPHLLMWCSEFMSTFCYGIMAQAIRSEVDKQLIERCSRIILNLARYNSTTVNTFQEGGLVTIAQMLLRWCDKDSEIFNTLCTLIWVFAHCPKKRKIIHDYMTNPEAIYMVRETKKLVARKEKMKQNARKPPPMTSGRYKSQKINFTPCSLPSLEPDFGIIRYSPYTFISSVYAFDTILCKLQIDMF

Target ORF information:

RefSeq Version	NM_079764.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster abnormal spindle (asp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079764.3

ORF Insert Sequence:

ATGAGCGCCT TTGAGATCAC AGTGACGCCA TCGCGCCTAA AGCAAAAGAA GCGCGCCGAA 
GGACGCGAGC CCGCCGTCGT CGTCATGGCT CCGTTCTCCG CCAAGGCGAT TGTGCAGTTC 
GAGGATGTGC CGATAACAAA GACAGCACGG CGCCAGGTGC GCGTTCTCAA TCCCAGCGAC 
GACGACATCG AGGTCAAGGT AATGAAGGCC ATCCGGGAGG AGCACAACTT AAGCCTGGAG 
TGGATGGAGC ACACGGTGCC TGCAAGAGAT GAGGTATCCA TGGAGCTAGT GTGGAGCCCC 
GTCCTTGAGG TGGCCTGCAA GGAGACGCTG CAGCTAATAG ACAACCGCAA CTTCCGAAAG 
GAGGTGATGA TCATACTCAA GTCCAAGAGC AACCAGCCGG TCAAGAACCC GCGCAAATTT 
CCTACTGTCG GCAAGACCCT GCAGCTGAAA TCGCCGACAG GAGCTGGCAA GACAATGAAA 
AGCGTGGTAT CCGCTGCTGT GCAGCAAAAG AAGCGCATGT CTGCAGCAGC AGCGCCTCCC 
TCCAAGCAGA CATGGCGAGT GACTGCTCCT TCGCGTCCCG CTGCATGGGC ACATCCACCT 
CCACAGGCTC CTCTTGTCGA GAAGAATGTA TACAAGACTC CACAAGAAGA GCCCGTATAC 
ATATCACCAC AGCCTCGCAG TCTTAAGGAA AATCTAAGCC CTATGACGCC AGGAAACCTA 
CTCGACGTGA TTGACAATCT GCGATTCACA CCTCTCACCG AAACCCGTGG CAAAGGACAA 
GCTACCATTT TCCCGGACAA TCTGGCAGCC TGGCCCACAC CAACACTTAA AGGGAATGTA 
AAATCATGTG CTAATGATAT GCGGCCGCGT CGAATCACTC CAGATGATCT AGAAGATCAG 
CCTGCCACAA ACAAAACGTT CGATGTAAAG CATTCCGAGA CTATCAATAT TTCGTTGGAC 
ACCTTGGACT GCTCCAGGAT CGATGGACAA CCGCATACGC CCCTAAATAA GACAACAACC 
ATTGTGCATG CCACGCACAC CAGAGCTCTG GCCTGTATTC ATGAGGAGGA GGGACCAAGT 
CCACCTAGGA CGCCCACGAA GAGCGCCATA CACGACCTGA AGAGGGACAT TAAGTTGGTG 
GGTTCACCCT TACGAAAGTA TTCCGAGTCC ATGAAAGATT TGTCACTTCT ATCGCCACAA 
ACTAAGTATG CCATTCAAGG GTCTATGCCT AATCTAAATG AAATGAAAAT CCGCTCGATC 
GAACAGAATA GATATTACCA GGAGCAGCAG ATCCAGATAA AGGCCAAAGA CTTGAATAGC 
TCCTCTAGTA GCGAGGCTAG TTTGGCCGGC CAGCAGGAGT TTCTATTCAA CCACAGTGAG 
ATCCTCGCTC AGTCCAGTCG TTTTAATCTC CATGAAGTAG GTCGGAAGTC GGTGAAGGGA 
AGTCCGGTGA AGAATCCTCA CAAGCGCCGC TCTCATGAGT TGAGTTTTTC GGATGCACCT 
AGCAACGAAT CATTGTACCG CAATGAAACT GTAGCCATTT CCCCTCCTAA AAAGCAACGG 
GTTGAGGACA CTACTCTGCC CAGAAGTGCA GCGCCGGCAA ATGCATCTGC AAGAAGCAGT 
AGTGCCCACG CCTGGCCACA CGCCCAATCC AAGAAGTTTA AGCTAGCACA AACCATGTCA 
CTGATGAAGA AGCCCGCCAC ACCACGAAAA GTCAGGGACA CTAGCATTCA GCCTTCCGTC 
AAGCTCTATG ACTCGGAGCT GTATATGCAG ACGTGCATCA ACCCGGATCC ATTTGCAGCA 
ACTACGACAA TTGATCCATT TCTGGCATCT ACCATGTATT TGGATGAACA GGCTGTGGAT 
CGTCATCAAG CTGACTTTAA AAAGTGGTTA AATGCCCTTG TCTCCATACC CGCTGACCTG 
GACGCAGATT TAAATAACAA AATAGACGTT GGTAAGCTGT TTAACGAGGT GCGCAACAAA 
GAGCTCGTGG TGGCTCCCAC CAAGGAGGAG CAGTCTATGA ACTACCTAAC GAAATACCGC 
CTGGAGACGC TTCGTAAGGC GGCTGTGGAG CTCTTCTTCA GTGAGCAGAT GCGCCTGCCA 
TGCTCCAAAG TGGCCGTATA TGTCAACAAG CAAGCTCTGC GCATCCGTAG CGATCGTAAT 
CTTCACCTAG ACGTAGTTAT GCAACGCACC ATACTTGAGC TGCTGCTTTG CTTCAATCCC 
CTTTGGCTGC GCCTTGGACT GGAAGTGGTC TTTGGCGAGA AGATCCAGAT GCAGTCTAAT 
CGAGACATTG TAGGCCTCAG CACCTTTATC CTCAATCGCT TGTTCCGGAA TAAGTGTGAG 
GAGCAGAGGT ACAGCAAGGC ATACACACTC ACCGAAGAGT ACGCGGAGAC CATTAAGAAG 
CACTCATTGC AGAAAATCCT CTTTTTGCTG CTTTTCCTCG ATCAAGCTAA GCAGAAGCGC 
ATCGTCAAGC ACAATCCCTG TTTGTTTGTT AAAAAGTCGC CACATAAAGA GACCAAGGAT 
ATTCTGCTGC GCTTCTCGTC GGAGCTGCTC GCCAACATTG GTGATATTAC GCGGGAACTT 
CGTCGCCTGG GCTACGTTCT ACAGCACCGC CAAACATTTT TGGACGAGTT CGATTATGCC 
TTCAACAACT TGGCTGTGGA CCTAAGAGAT GGCGTGAGAC TAACCCGAGT CATGGAGGTA 
ATTTTACTAC GCGATGATCT AACCCGCCAG TTAAGGGTGC CAGCCATCTC TCGCCTTCAG 
CGGATCTTCA ATGTAAAGCT AGCTCTGGGC GCACTTGGTG AAGCCAACTT CCAGCTAGGC 
GGCGACATCG CCGCCCAAGA CATCGTTGAC GGACATCGTG AGAAGACGCT TTCCCTGCTC 
TGGCAACTTA TTTACAAATT CCGCTCGCCC AAGTTTCATG CGGCGGCCAC GGTGCTCCAG 
AAATGGTGGC GCCGTCACTG GCTGCACGTT GTTATCCAGC GTCGCATTCG CCACAAAGAG 
CTTATGCGTC GCCACCGGGC CGCTACTGTC ATTCAGGCCG TGTTCCGTGG CCACCAGATG 
AGAAAGTACG TGAAGTTGTT TAAGACGGAA CGCACTCAGG CCGCAATAAT TCTGCAAAAG 
TTCACCCGTC GGTATTTGGC CCAGAAGCAG CTATATCAGA GCTATCACAG TATTATCACC 
ATCCAGCGCT GGTGGCGAGC CCAACAACTG GGAAGGCAGC ACCGCCAGCG GTTTGTGGAG 
CTCCGAGAGG CTGCGATCTT TCTTCAGCGG ATCTGGCGGC GACGACTCTT TGCCAAAAAA 
CTATTGGCGG CGGCGGAAAC AGCCAGACTT CAGCGATCGC AAAAACAACA GGCAGCTGCT 
AGTTATATTC AAATGCAATG GCGAAGCTAT CAGCTGGGCA GAATTCAGCG ACAGCAGTTC 
CTGCGCCAGA GGGACCTCAT CATGTTTGTT CAGCGCAGGA TGCGAAGCAA GTGGAGTATG 
CTGGAGCAGC GCAAGGAGTT CCAGCAACTA AAGCGTGCAG CTATAAATAT CCAACAACGC 
TGGCGAGCGA AGCTTTCAAT GAGAAAGTGC AACGCTGATT ATTTGGCACT TCGTTCCAGC 
GTTCTTAAAG TTCAGGCTTA CAGGAAAGCC ACAATCCAGA TGAGAATAGA TCGTAATCAC 
TACTATTCCC TGCGAAAAAA TGTTATCTGC CTGCAACAGC GACTGAGGGC CATCATGAAA 
ATGCGCGAAC AGAGGGAAAA TTATCTGAGG CTGCGAAATG CTTCAATACT AGTTCAAAAA 
CGCTACCGCA TGCGTCAACA AATGATCCAG GATAGAAATG CATATTTAAG AACCCGCAAA 
TGTATCATCA ATGTCCAGAG GCGCTGGAGA GCCACTCTGC AGATGCGTCG AGAAAGGAAG 
AACTACCTTC ATCTCCAAAC GACAACCAAA CGAATTCAAA TCAAGTTCCG TGCCAAGCGC 
GAAATGAAAA AGCAAAGAGC CGAGTTTCTT CAGCTGAAAA AGGTTACCCT TGTAGTCCAG 
AAACGTCGGC GGGCTTTGCT GCAGATGCGA AAGGAGCGCC AGGAGTACCT ACATCTGCGC 
GAGGTTACCA TAAAGCTGCA GCGCAGATTC CATGCTCAAA AATCAATGCG GTTCATGCGA 
GCCAAGTACC GCGGCACCCA GGCTGCTGTG AGCTGCCTGC AGATGCATTG GCGTAATCAT 
CTCCTTAGGA AACGGGAGAG GAACAGTTTC TTACAACTGC GTCAAGCAGC AATAACACTG 
CAGCGACGGT ACCGAGCTCG TCTGAATATG ATCAAGCAGT TGAAGAGTTA CGCCCAGCTG 
AAACAGGCAG CTATTACCAT TCAAACCCGA TATAGAGCCA AGAAGGCAAT GCAAAAGCAG 
GTGGTCTTGT ACCAAAAGCA AAGGGAAGCC ATTATCAAAG TGCAACGACG ATACCGCGGC 
AATCTGGAGA TGAGGAAGCA GATTGAAGTC TACCAAAAAC AGCGCCAGGC AGTCATCCGC 
TTGCAGAAAT GGTGGCGCAG TATACGCGAC ATGCGGCTGT GCAAAGCGGG CTACCGAAGG 
ATTCGACTCA GTTCATTGAG CATTCAACGC AAGTGGCGGG CCACAGTGCA GGCTCGTCGC 
CAACGGGAGA TCTTCTTAAG CACCATCCGC AAAGTGCGAC TTATGCAGGC ATTTATCAGA 
GCAACTTTAC TGATGCGTCA GCAGCGCAGG GAATTCGAGA TGAAGCGAAG GGCTGCCGTA 
GTGATCCAGC GCCGGTTTCG TGCTCGCTGT GCTATGCTAA AGGCGAGACA GGATTACCAA 
TTAATCCAAT CCTCTGTGAT CCTGGTGCAG CGCAAATTCC GTGCTAATCG CAGCATGAAG 
CAGGCACGCC AGGAATTTGT CCAGCTGCGT ACTATTGCAG TCCATTTGCA ACAAAAGTTC 
CGTGGCAAGC GTCTAATGAT TGAGCAGCGT AATTGTTTCC AACTGCTCCG CTGTTCCATG 
CCGGGCTTCC AGGCACGTGC CCGCGGCTTT ATGGCTCGCA AACGATTCCA GGCCCTGATG 
ACACCCGAGA TGATGGACCT CATCCGCCAG AAGCGCGCCG CCAAGGTTAT ACAGCGTTAC 
TGGCGGGGCT ATCTAATCCG ACGACGTCAG AAGCACCAGG GTCTCTTGGA TATCCGGAAG 
CGTATTGCTC AGCTGCGGCA AGAGGCAAAG GCGGTAAACT CTGTGCGCTG CAAAGTCCAG 
GAGGCGGTGC GTTTCCTTCG CGGACGCTTT ATCGCATCAG ATGCGTTAGC AGTGCTAAGT 
CGATTGGATC GTCTTTCGCG CACTGTGCCA CACCTGCTCA TGTGGTGTTC GGAGTTCATG 
TCCACGTTTT GCTATGGCAT CATGGCTCAG GCCATTCGAT CAGAGGTGGA TAAGCAGCTT 
ATAGAGCGCT GCAGCCGGAT CATCCTAAAT TTGGCCCGCT ACAATAGCAC CACGGTGAAC 
ACGTTCCAGG AGGGCGGTTT GGTCACCATT GCCCAGATGT TATTGCGCTG GTGCGACAAA 
GACAGTGAGA TATTCAACAC TTTGTGCACC CTCATTTGGG TATTCGCCCA CTGTCCTAAA 
AAGCGAAAGA TCATTCACGA CTATATGACC AACCCAGAGG CCATTTACAT GGTGCGCGAA 
ACTAAGAAGC TCGTGGCCCG CAAGGAAAAG ATGAAGCAAA ATGCACGCAA GCCGCCGCCA 
ATGACAAGTG GACGGTATAA GAGCCAAAAG ATAAACTTCA CGCCGTGTTC CCTGCCCAGC 
CTGGAGCCGG ACTTCGGAAT CATCCGCTAC AGTCCCTACA CGTTTATCTC GTCCGTTTAC 
GCCTTCGATA CGATTTTGTG CAAGCTGCAG ATCGACATGT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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asp cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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