BRWD3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRWD3
Entrez Gene ID	42898
Full Name	CG31132 gene product from transcript CG31132-RA
Synonyms	Brwd3,CG18670,CG31132,CG6400,Dmel\CG31132,anon-WO0118547.520,anon-WO0118547.620,brwd3,dBRWD3,l(3)05842,l(3)s5349,psg13,ram
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95F12-95F13\|3-79.8 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_170139.3	NP_732982.1	BRWD3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our findings identified dBRWD3 as a critical regulator that is uniquely required for ectopic gene expression and aberrant tissue overgrowth caused by PcG mutations.
Title: dBRWD3 Regulates Tissue Overgrowth and Ectopic Gene Expression Caused by Polycomb Group Mutations.

Intellectual disability-associated BRWD3 regulates gene expression through inhibition of HIRA/YEM-mediated chromatin deposition of histone H3.3.
Title: Intellectual disability-associated dBRWD3 regulates gene expression through inhibition of HIRA/YEM-mediated chromatin deposition of histone H3.3.

BRWD3 functions as a Damage-specific DNA binding protein 1 (DDB1)- and CULLIN (CUL)4-associated factor in a Cullin4-RING Finger E3 Ligase (CRL4) that mediates light-dependent binding of dCRY to CUL4-ROC1-DDB1-BRWD3, inducing ubiquitylation of dCRY
Title: Ramshackle (Brwd3) promotes light-induced ubiquitylation of Drosophila Cryptochrome by DDB1-CUL4-ROC1 E3 ligase complex.

In the developing retina, ramshackle (ram) mutant cells have morphological defects including disrupted apical junctions, disorganized actin cytoskeletons and mislocalized nuclei.
Title: The Drosophila ramshackle gene encodes a chromatin-associated protein required for cell morphology in the developing eye.

The following BRWD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRWD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05937	NM_170139.3 Latest version!	Drosophila melanogaster BRWD3 (BRWD3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRWD3 ( NM_170139.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732982.1 Drosophila melanogaster BRWD3 (BRWD3), mRNA.

Price & Availability↑

CloneID	OFa05937
Clone ID Related Accession (Same CDS sequence)	NM_170139.3
Accession Version	NM_170139.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	BRWD3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170139.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAACTA GACAACCCAG TTCCGGTGAT GATCTAAAGC CAGAGCTGTA CTTCCTCATT 
TCCAAGTTCC TGGCCGCGGG ACCGCTGGAG GAGACGGCGA AGGTCTTGAT CCGGGAACTC 
GAAGAGAAGA AGGTTTTGCC GCGTCGTCTC GATTGGCAGG GAAACGAGCA TGAGCAGACC 
TTTGAGGAAT TGGAACGAAA GTACAAGCAC ATTGGCGCCA ATCACTTGCT TGAAATATGC 
AGTCGTCTGG GTCCACTGGT GGATCGCGAG CTTCCGCCCA GTGTTCCGGG CATCAACTCG 
CTGTTGGGTA CTGGGCGCCA GAACCTCCTG CGCACCAAGG AAACCGTCTA CTGCCATCGC 
TCCTTAAGGG ACTACTGCAC CCGCCTGAAT GGAGTCTCGC TGCCGGATTC GGTCCTTACA 
AAGCCGACCC ACAACTTAGA TCGCGTTCTA ACTGGACGGG AACACGGCGG GGAAGTCCGT 
CGCAAGCTCC TCGTGCCCAC GGACCTCTAC CGCCGAACAA AATTGCTCCG TCGCACTGTG 
GGCCACCTGT CCTCAGTGTA CTGTGTTCTG TTTGATCGCA CAGGAAGATA CATCATCACC 
GGAGCCGATG ATCTACTTAT TAAGATATGG TCAGCGGCAG ATGGCCGCCT GCTGGCCACT 
CTGCGAGGCG CCTCATCCGA GATTACAGAC ATAGCCATTA ACTTGGACAA TACGATGCTG 
GCTGCCGGGT CATTGGATCA CATACTAAGA GTGTGGGACA TGCAAACCAC ATCACCCATC 
GCCGTACTGT CTGCTCACAC GGGCATGATA ACTTCGGTTA ACTTTTGTCC GTCGCCACGT 
AGCGATCTGA AATATCTGGT GACTACCAGC ACAGATGGAT CGATCGCCTT CTGGCAATAC 
TCAACGCCGC GCGGGCAAAA GATCACGTTT GCCCCGAAGC CAACGCAGTA TCATGAGAAA 
CTGCGGCCCG GACAAGCTCA GATGATGTGT ACCACCTTTT CGCCAGGAGG CATTTTTCTG 
GCCGCTGGGT CTGCCGATCA CCATGTGCGC GTCTATATGA TGGGCGAGGA CGGTCCGAAG 
CGAATATTGG AGACGGAAGC TTACACAGAT GCCGTGGACT CGGTGCAGTG GTCGCATCGT 
GGACTGAGAT TTATATCGGG GAGCAAGGAC GGCACAGCAC ACATCTGGAC GTTTGAGTCG 
CAGCAGTGGA AAAGTTCCAA ACTATGCATG ACGGAGCGTC TGGCCAGCTG CCCGGAACCA 
GAGGAGGGAA AGAGGCTTAA GGTAACCATG GTGGCTTGGG ATGCCTCGGA CCGTTATGTC 
ATCACCGCCG TAAACGATTT TACTATAAAA ATCTGGGACT CGAAGTCAGC CAAGCTGCAT 
CGTGTTCTGC GTGGGCACAA GGATGAGCTT TATGTGCTTG AGTCGAACCC AAGAGATGAG 
CATGTCCTGC TTTCCGCCGG GCACGATGGC CAGGTATTTC TGTGGGACAT CGAGCAGGGC 
GTTTGTGTGG CCAACTTCCT TAACGACATC GATGGCCAAG GCCATGGCAG TGTCTTTGAT 
GCAAAGTGGT CACCGGATGG CACAATGATA GCTGCCACCG ATTCGCATGG ACACATCCTG 
ATTTTTGGTT TGGGCGTGTG CATTGAAAAG TACAAAATGC TTCCCACGGA GCTGTTCTTT 
CACACGGATT TTCGACCTTT ATTGAGAGAT GCGCAGCACC ATGTGGTGGA CGAGCAGACG 
CAGATAATGC CGCACCTAAT GCCCCCGCCT TTCCTGGTCG ATGCAGATGG CAACCCGCAT 
CCATCAAGGT TTCAACGATT TGTGCCTGGT CGTGAGAGCT GTAGCCTAGA CCAATTGATT 
CCAAATCTTA CGGTAGGCGT GGATGGCGTA GTAATCGACG ATGGAGGTGC AGGTGGAGAT 
GCTGTTGTGG CCGCTGCACC CGCTGAAGCT CCGCCAGCCG CATTGCCCCC TGCTGCTGCT 
GGAGGACCAC AGGGAGCTAA CTACTCCCAT ATCGACCGCA TGATTGCAGC CTTGGCCAAT 
CGGCAGTCGG TTAATGTAAA TGCCAATGAT GCCAATCCGA CCAACGGCAA CCAGTCCTTC 
GAACGTTTGA CCAATCGTCA AATGCCGGAC TCCAATGGCA GGCAATCGTC ACGTGGCAAT 
GTCCTGGGTA GTCGTTTGAG CACGCCTCGC CAAGGATGGG GTGCCGCAGC TGCCGCGCCA 
GTTGAGGATC AAGCAGGGCC GTCTCAACCT CCGGCAGAAC CTGCAGCCGA ACCTGGCCAG 
GCACAAGTCC AACAGGCGCT GCCTCTGAAA TTCATCCGTC GCACTTATGT GCGTCCCATG 
AAGTATCCCC AGTTGCAAAA CCTTAAGCAG ACTATTTACT CTGCCGGTCA GTTTGAAAAG 
CAGGAGTACA AACGCGAAAT GCGACGCCGA CCCATCATGA TAAACACTGC CAGTGCAGCA 
TCTTCGCAGC AGCCAGGAAG CGTTGGCAGG CCACGAAACA CACGTGGCAA TGGCGGCGGC 
GCAAGAGCTG GACGAAGACG TGGCCCCCAG CCAGCTGGCG GACAGCCACA ACCCGCTTAT 
CGCACCCGCG CTGTTCGGGA TCAGGAGCCG GAGCACTACG ATGAGGTAGC GCCGCCACCC 
GAGGAAGAGG AGGAAGATGT GTCCAGCAAC TCGTCCGGCG ACACGAGCTA CTCGAATGTG 
GAAGAAAACC TAGAGGACAG CAGCGATGAG TCGGAAACGG ACAGTTCCGA TTACTCTGAC 
TGGGTGGCGG ATACTCCTGG CCCGAATCTG GAACCACCAA AACGATCCAA GCGTAAGCCA 
CTCTCACGGC GACCCCGGAG TAGTGATGAC AGCAGCGATG ATGAGCGAGC GGGCAGATCC 
AGAGGTGGGC AGGTTACGGC CCGGAAGGTT GGACGTAAAA CTGTTTTGTT TCCGCCCACA 
GATGCAAATG GAGAGATACC GGAGCTTTAT CGTCCCGCTG AGTGGCTATC TGAGGTGATT 
CCACGTAAGG CACCCTATTA CCCCCAGATG GGTGATGAGG TAGTCTACTT CCGGCAAGGA 
CACGCAAAGT ACTTGGATGC AGTCCGCCTG AAAAAGGTCT ACAAGCTCTC GCACAGCTCG 
GAGCCGTGGA ACTTTCACAC ACTGCGCGAC CACGAGCTGG TGCGTGTGAT TGGAATCAAG 
TACGAAATCC GCCCACCCCG ATTATGTTGC TTGAAGTTGG CCATCATCGA TGAGGAGGGC 
AACATGACGG GGACAACGTT CAAGATCAAG TACCATGACA TGCCCGACGT TTTGGACTTT 
TTAGTGCTGC GGCAGACCTT CGATCTTGCA GTGCAGCGCA ACTGGAGCAT TGGTGACCGG 
TTTCGCTGCA TGATTGTCGA TGGCTGGTGG ATGGGTCAGA TTGAATCGCG CCACGCCCTC 
TCTGCTGACT TCCCCGACTC GTCCTTCATG TGTTTCCGCG TGCGCTGGGA TAATGGAGAG 
TATGAGTTTA TGAGTCCCTG GGACATGGAA CCCATCGATC AGCAAAGAGT ACCGGACGAG 
GTGGGAGGTG CTGTTCCAGT TCTACCGGAG GAGATTCGCG CCACTCTATA CCAGCCCAAG 
TCCGAGGAAT GGCACAGAGG GGATCGCGAT GGCACCTGCA GGCGAATTAT CAATGGATTG 
GAGCAGGTTA TGCGTCTCTC TATCGCAGAG CATTTCCTGG CCCCCGTGGA CCTTAACATA 
TATCCAGACT ACGCTTATTT GATCGAATAT CCCATTGACC TGACCACAAT TAAATCTCGC 
TTTGAGAATC ACTTCTACCG CAGAATAACC TCAGCCCAGT TCGATGTACG CTACCTAGCC 
ACCAATGCGG AGCAGTACAA TCGCCGACAC GCAATTATCG TCAAGCATTC GCGAATTGTG 
ACAGATCTTT GTTTGAGGAT CATCAGGGAA CCGGATGAGA TAGATGTGGC TGCTGTTTAC 
CATCAGCTTG CGGATGTGTA CCATTCGTCC GAGTCGGAGA ACGAGGTAGA TTCGGATGTA 
GTGCCATCGA CCAGCACAGG GCCAACTACT TCAGCAGCTG CAGCAGCAGC TAGGCAAAGA 
GTATCCTCAG CCAGGCGATC CACTCGAATT CACTCGGAAG GAGACTGGCG TGCCGATTGT 
CGTCAGCTGC TTGATCTTAT GTGGGCGAGG ACAGATTCGG CGCCGTTCCG CGAGCCCGTT 
GACACCATCG ACTTCCCTGA CTACCTGGAG ATCATAGCTA CTCCAATGGA TCTGCGCACT 
GTAAAGGAGG ATCTGCTGGG CGGCAACTAC GATGATCCGT TGGACTTTGC CAAGGATGTG 
CGGCTGATCT TCCAAAACTC CAAGAACTAC AACACCAACA AGCGCTCACG GATTTATGCC 
ATGACCTTGC GACTCAGTGC GCTATTTGAG TCGCATATCA AGACAGTGGT CACCAGCTGG 
AAGGCGGCAT TAAGGCGCGC TAATAAAAAC AAGTCCGGCA GCAGTGGAGG AGGACGCGGT 
GGTTCCAGCA GCAGTCCCAA CAAGCGAGTC AAAAAGGAGC GATCCACGCG CCGTAATCAA 
AAGCCAACAG CAGCGGCCAG AAGATTGCCA GTCGCCAACT CCAGTGAAGA TGAGGACGAG 
GAGGATGATG ATGATGACGA CGATGATGAT GATGAGGAAG AGGCATCAAC GAGGGGGCGC 
TCTCGTCGGC GAAACGGTGT GGCCGTAAGT GCTAGTTCCT CTGGCTCCAA TCGCGTGACT 
TCTTCATCCG CCGCCCAACA GCGTCGAGGC AAAAGCAGCA GTAGCGAGGA GGAAGAGGAG 
GAGGAAACCA GTGCGCAGGC CACGTCGGAT GCCAGCAGCG ATGAAGAGGA AAGAGCCCAA 
TCCAATGACA GTTCGCCCCA GCAGACACGC ACGCGCAGGG CAAGGCAGCG GCAACGGCTG 
AGCGATGACT CCAATATCAA CGATTCCGAG GATAGTTACA ACCCCAACGA GGGACGCGGC 
AGTCGGCGAC GTCCAGGTGC CGCACGCCCG TCCAGCAATA GGCAGACAAA TGGCCACTCC 
AGCAAGAGGC GACGTTTGAA CAAAAGTCCA GCCAATGCTG CACCTGGCCC TAGTTTGCCA 
CGTCGGACAC GTCGCATAGC AGGCAGCTCC GCAGAAGAGG CTGCATCTCA CTCACAACAC 
GATGAGAGCA CACAGGATAG CCAGATAGGA CGTCGAAAGG GACGCTCGGC AAGCAGCAGG 
TCAATTGGCA ATGGACCAAG TACCAGTGCC GCAGCTGCCG CCAACTCCAG CCTTGAAAGT 
CCGTCACGGA ATACTCGAGC CAACGGAACC AGGACCTCTT CGCATCTTGG AGCCGAAAAT 
GATCACAGTT ACCACCTGCC AGTGCGCAAC GGGCGCATTA TCGAGTCCGA TACGGACTCT 
CCTGTCATCA GACCCACGCG ACAGAGCGCC AAGAGGGCGC TAATGGACTG GGGTCGTGGA 
AGCGATATTG AAGAGGAGGA GGAGGAAGAG GAAGAAGACA GCGAGGAAGC CGAGGAGATA 
CTGCCAACAC CTACAAAGGA CGACTTGCCC TCTACCAGCC GAGCCGCTTT GGCTCAGGCG 
GCTGCTGGTC CTTCTCGCCA GCTACGCACG AACCGCAACA CCGTCGTTCG TCCAGCCCAG 
AGTGACGACG ATGACGACGA CGACAGTGAC AACGAGCCGC TGGCCAACAA TCAACAAAAA 
AATTATCAAT CACCTGCAAA ATCTAGCTCA ACCGCAACAG TGGCCCCTCA TCCACTGAGC 
CTGCGCCGTC GATTAGCCTC GCCGCCAGAA CGGAATACCC ATATGACCCG TTCCCATGCC 
ACCTCCACAA CGTCCTCGGC CGCAGCATCA CGGGCAGAAC AACAACCAGC GGTTTCCACC 
CACGATCACA ACTACCTAGG CAACGTGAAT TCATCGAGGG CACCGCGCCG CATTCTTTCA 
CGCCACCAAC GAAATGCCGA CGAGTTGGAT ACCAGTCTAG ATGTCCTGGA AAACTCGCGT 
CTGCTCTCAG CCATAGCCAA TCACCGGCGA CCCACCTCTA CCACGAACAG CAACAGTGCG 
AGCAACGTGG TTACAAGGCG TCTGCGTGGT CGCGCTAGCC AGGCGAATTC GCACGACGAG 
GATCATGAGG AAGAGGAGGC TGCTGCTTCC GATGAGGGCA CTGATGGAGG CTCTAGCTCG 
CAGAGCTACG AAGATGAGGA TGAAGACTCC GACTCGGAAG ACAATCAGCC GCTGACCAGC 
TACGTATCGC CCAGCAATGG TCGTCCGAGG CGAAAGGCAA AAACCTCCTC CTCCTCCGCA 
ACAACTCAGG AGCGTCCGCT GCGACGCACA CGTCGCCCGC CATCCAATAA CTCCATCGCG 
AACGACAACG ATGACGATGA TGACTATGTT GTGCCGCGGA GTCGTCGCGC CAACCGGGGT 
GGAAACCAGC GAGCCGGGCG CAACCAAAAG AGACCGCGCT ACAATGAGCA GTCCGAGGAG 
GAAGAGGCGG CGGCTGGATA TTCCCATCGG AGTAAACGAA TCCGTAACGG CGACGCTCAT 
GCCAGCTCTC AGGAGCGATC ACAGGCCTCC ACCGAGCGGG ACCAGGAGGA AGAAGAGGAC 
GGTACTTCGG TGGACTCGGA AGAACAGCAG GTGAACGTTA GCAGCAGAGG CAGGGTGCGC 
AAGCCGTCTG CCAAGGCGCG CGGTATCTTC AAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732982.1
CDS	116..6814
Translation	METRQPSSGDDLKPELYFLISKFLAAGPLEETAKVLIRELEEKKVLPRRLDWQGNEHEQTFEELERKYKHIGANHLLEICSRLGPLVDRELPPSVPGINSLLGTGRQNLLRTKETVYCHRSLRDYCTRLNGVSLPDSVLTKPTHNLDRVLTGREHGGEVRRKLLVPTDLYRRTKLLRRTVGHLSSVYCVLFDRTGRYIITGADDLLIKIWSAADGRLLATLRGASSEITDIAINLDNTMLAAGSLDHILRVWDMQTTSPIAVLSAHTGMITSVNFCPSPRSDLKYLVTTSTDGSIAFWQYSTPRGQKITFAPKPTQYHEKLRPGQAQMMCTTFSPGGIFLAAGSADHHVRVYMMGEDGPKRILETEAYTDAVDSVQWSHRGLRFISGSKDGTAHIWTFESQQWKSSKLCMTERLASCPEPEEGKRLKVTMVAWDASDRYVITAVNDFTIKIWDSKSAKLHRVLRGHKDELYVLESNPRDEHVLLSAGHDGQVFLWDIEQGVCVANFLNDIDGQGHGSVFDAKWSPDGTMIAATDSHGHILIFGLGVCIEKYKMLPTELFFHTDFRPLLRDAQHHVVDEQTQIMPHLMPPPFLVDADGNPHPSRFQRFVPGRESCSLDQLIPNLTVGVDGVVIDDGGAGGDAVVAAAPAEAPPAALPPAAAGGPQGANYSHIDRMIAALANRQSVNVNANDANPTNGNQSFERLTNRQMPDSNGRQSSRGNVLGSRLSTPRQGWGAAAAAPVEDQAGPSQPPAEPAAEPGQAQVQQALPLKFIRRTYVRPMKYPQLQNLKQTIYSAGQFEKQEYKREMRRRPIMINTASAASSQQPGSVGRPRNTRGNGGGARAGRRRGPQPAGGQPQPAYRTRAVRDQEPEHYDEVAPPPEEEEEDVSSNSSGDTSYSNVEENLEDSSDESETDSSDYSDWVADTPGPNLEPPKRSKRKPLSRRPRSSDDSSDDERAGRSRGGQVTARKVGRKTVLFPPTDANGEIPELYRPAEWLSEVIPRKAPYYPQMGDEVVYFRQGHAKYLDAVRLKKVYKLSHSSEPWNFHTLRDHELVRVIGIKYEIRPPRLCCLKLAIIDEEGNMTGTTFKIKYHDMPDVLDFLVLRQTFDLAVQRNWSIGDRFRCMIVDGWWMGQIESRHALSADFPDSSFMCFRVRWDNGEYEFMSPWDMEPIDQQRVPDEVGGAVPVLPEEIRATLYQPKSEEWHRGDRDGTCRRIINGLEQVMRLSIAEHFLAPVDLNIYPDYAYLIEYPIDLTTIKSRFENHFYRRITSAQFDVRYLATNAEQYNRRHAIIVKHSRIVTDLCLRIIREPDEIDVAAVYHQLADVYHSSESENEVDSDVVPSTSTGPTTSAAAAAARQRVSSARRSTRIHSEGDWRADCRQLLDLMWARTDSAPFREPVDTIDFPDYLEIIATPMDLRTVKEDLLGGNYDDPLDFAKDVRLIFQNSKNYNTNKRSRIYAMTLRLSALFESHIKTVVTSWKAALRRANKNKSGSSGGGRGGSSSSPNKRVKKERSTRRNQKPTAAARRLPVANSSEDEDEEDDDDDDDDDDEEEASTRGRSRRRNGVAVSASSSGSNRVTSSSAAQQRRGKSSSSEEEEEEETSAQATSDASSDEEERAQSNDSSPQQTRTRRARQRQRLSDDSNINDSEDSYNPNEGRGSRRRPGAARPSSNRQTNGHSSKRRRLNKSPANAAPGPSLPRRTRRIAGSSAEEAASHSQHDESTQDSQIGRRKGRSASSRSIGNGPSTSAAAAANSSLESPSRNTRANGTRTSSHLGAENDHSYHLPVRNGRIIESDTDSPVIRPTRQSAKRALMDWGRGSDIEEEEEEEEEDSEEAEEILPTPTKDDLPSTSRAALAQAAAGPSRQLRTNRNTVVRPAQSDDDDDDDSDNEPLANNQQKNYQSPAKSSSTATVAPHPLSLRRRLASPPERNTHMTRSHATSTTSSAAASRAEQQPAVSTHDHNYLGNVNSSRAPRRILSRHQRNADELDTSLDVLENSRLLSAIANHRRPTSTTNSNSASNVVTRRLRGRASQANSHDEDHEEEEAAASDEGTDGGSSSQSYEDEDEDSDSEDNQPLTSYVSPSNGRPRRKAKTSSSSATTQERPLRRTRRPPSNNSIANDNDDDDDYVVPRSRRANRGGNQRAGRNQKRPRYNEQSEEEEAAAGYSHRSKRIRNGDAHASSQERSQASTERDQEEEEDGTSVDSEEQQVNVSSRGRVRKPSAKARGIFKE

Target ORF information:

RefSeq Version	NM_170139.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster BRWD3 (BRWD3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170139.3

ORF Insert Sequence:

ATGGAAACTA GACAACCCAG TTCCGGTGAT GATCTAAAGC CAGAGCTGTA CTTCCTCATT 
TCCAAGTTCC TGGCCGCGGG ACCGCTGGAG GAGACGGCGA AGGTCTTGAT CCGGGAACTC 
GAAGAGAAGA AGGTTTTGCC GCGTCGTCTC GATTGGCAGG GAAACGAGCA TGAGCAGACC 
TTTGAGGAAT TGGAACGAAA GTACAAGCAC ATTGGCGCCA ATCACTTGCT TGAAATATGC 
AGTCGTCTGG GTCCACTGGT GGATCGCGAG CTTCCGCCCA GTGTTCCGGG CATCAACTCG 
CTGTTGGGTA CTGGGCGCCA GAACCTCCTG CGCACCAAGG AAACCGTCTA CTGCCATCGC 
TCCTTAAGGG ACTACTGCAC CCGCCTGAAT GGAGTCTCGC TGCCGGATTC GGTCCTTACA 
AAGCCGACCC ACAACTTAGA TCGCGTTCTA ACTGGACGGG AACACGGCGG GGAAGTCCGT 
CGCAAGCTCC TCGTGCCCAC GGACCTCTAC CGCCGAACAA AATTGCTCCG TCGCACTGTG 
GGCCACCTGT CCTCAGTGTA CTGTGTTCTG TTTGATCGCA CAGGAAGATA CATCATCACC 
GGAGCCGATG ATCTACTTAT TAAGATATGG TCAGCGGCAG ATGGCCGCCT GCTGGCCACT 
CTGCGAGGCG CCTCATCCGA GATTACAGAC ATAGCCATTA ACTTGGACAA TACGATGCTG 
GCTGCCGGGT CATTGGATCA CATACTAAGA GTGTGGGACA TGCAAACCAC ATCACCCATC 
GCCGTACTGT CTGCTCACAC GGGCATGATA ACTTCGGTTA ACTTTTGTCC GTCGCCACGT 
AGCGATCTGA AATATCTGGT GACTACCAGC ACAGATGGAT CGATCGCCTT CTGGCAATAC 
TCAACGCCGC GCGGGCAAAA GATCACGTTT GCCCCGAAGC CAACGCAGTA TCATGAGAAA 
CTGCGGCCCG GACAAGCTCA GATGATGTGT ACCACCTTTT CGCCAGGAGG CATTTTTCTG 
GCCGCTGGGT CTGCCGATCA CCATGTGCGC GTCTATATGA TGGGCGAGGA CGGTCCGAAG 
CGAATATTGG AGACGGAAGC TTACACAGAT GCCGTGGACT CGGTGCAGTG GTCGCATCGT 
GGACTGAGAT TTATATCGGG GAGCAAGGAC GGCACAGCAC ACATCTGGAC GTTTGAGTCG 
CAGCAGTGGA AAAGTTCCAA ACTATGCATG ACGGAGCGTC TGGCCAGCTG CCCGGAACCA 
GAGGAGGGAA AGAGGCTTAA GGTAACCATG GTGGCTTGGG ATGCCTCGGA CCGTTATGTC 
ATCACCGCCG TAAACGATTT TACTATAAAA ATCTGGGACT CGAAGTCAGC CAAGCTGCAT 
CGTGTTCTGC GTGGGCACAA GGATGAGCTT TATGTGCTTG AGTCGAACCC AAGAGATGAG 
CATGTCCTGC TTTCCGCCGG GCACGATGGC CAGGTATTTC TGTGGGACAT CGAGCAGGGC 
GTTTGTGTGG CCAACTTCCT TAACGACATC GATGGCCAAG GCCATGGCAG TGTCTTTGAT 
GCAAAGTGGT CACCGGATGG CACAATGATA GCTGCCACCG ATTCGCATGG ACACATCCTG 
ATTTTTGGTT TGGGCGTGTG CATTGAAAAG TACAAAATGC TTCCCACGGA GCTGTTCTTT 
CACACGGATT TTCGACCTTT ATTGAGAGAT GCGCAGCACC ATGTGGTGGA CGAGCAGACG 
CAGATAATGC CGCACCTAAT GCCCCCGCCT TTCCTGGTCG ATGCAGATGG CAACCCGCAT 
CCATCAAGGT TTCAACGATT TGTGCCTGGT CGTGAGAGCT GTAGCCTAGA CCAATTGATT 
CCAAATCTTA CGGTAGGCGT GGATGGCGTA GTAATCGACG ATGGAGGTGC AGGTGGAGAT 
GCTGTTGTGG CCGCTGCACC CGCTGAAGCT CCGCCAGCCG CATTGCCCCC TGCTGCTGCT 
GGAGGACCAC AGGGAGCTAA CTACTCCCAT ATCGACCGCA TGATTGCAGC CTTGGCCAAT 
CGGCAGTCGG TTAATGTAAA TGCCAATGAT GCCAATCCGA CCAACGGCAA CCAGTCCTTC 
GAACGTTTGA CCAATCGTCA AATGCCGGAC TCCAATGGCA GGCAATCGTC ACGTGGCAAT 
GTCCTGGGTA GTCGTTTGAG CACGCCTCGC CAAGGATGGG GTGCCGCAGC TGCCGCGCCA 
GTTGAGGATC AAGCAGGGCC GTCTCAACCT CCGGCAGAAC CTGCAGCCGA ACCTGGCCAG 
GCACAAGTCC AACAGGCGCT GCCTCTGAAA TTCATCCGTC GCACTTATGT GCGTCCCATG 
AAGTATCCCC AGTTGCAAAA CCTTAAGCAG ACTATTTACT CTGCCGGTCA GTTTGAAAAG 
CAGGAGTACA AACGCGAAAT GCGACGCCGA CCCATCATGA TAAACACTGC CAGTGCAGCA 
TCTTCGCAGC AGCCAGGAAG CGTTGGCAGG CCACGAAACA CACGTGGCAA TGGCGGCGGC 
GCAAGAGCTG GACGAAGACG TGGCCCCCAG CCAGCTGGCG GACAGCCACA ACCCGCTTAT 
CGCACCCGCG CTGTTCGGGA TCAGGAGCCG GAGCACTACG ATGAGGTAGC GCCGCCACCC 
GAGGAAGAGG AGGAAGATGT GTCCAGCAAC TCGTCCGGCG ACACGAGCTA CTCGAATGTG 
GAAGAAAACC TAGAGGACAG CAGCGATGAG TCGGAAACGG ACAGTTCCGA TTACTCTGAC 
TGGGTGGCGG ATACTCCTGG CCCGAATCTG GAACCACCAA AACGATCCAA GCGTAAGCCA 
CTCTCACGGC GACCCCGGAG TAGTGATGAC AGCAGCGATG ATGAGCGAGC GGGCAGATCC 
AGAGGTGGGC AGGTTACGGC CCGGAAGGTT GGACGTAAAA CTGTTTTGTT TCCGCCCACA 
GATGCAAATG GAGAGATACC GGAGCTTTAT CGTCCCGCTG AGTGGCTATC TGAGGTGATT 
CCACGTAAGG CACCCTATTA CCCCCAGATG GGTGATGAGG TAGTCTACTT CCGGCAAGGA 
CACGCAAAGT ACTTGGATGC AGTCCGCCTG AAAAAGGTCT ACAAGCTCTC GCACAGCTCG 
GAGCCGTGGA ACTTTCACAC ACTGCGCGAC CACGAGCTGG TGCGTGTGAT TGGAATCAAG 
TACGAAATCC GCCCACCCCG ATTATGTTGC TTGAAGTTGG CCATCATCGA TGAGGAGGGC 
AACATGACGG GGACAACGTT CAAGATCAAG TACCATGACA TGCCCGACGT TTTGGACTTT 
TTAGTGCTGC GGCAGACCTT CGATCTTGCA GTGCAGCGCA ACTGGAGCAT TGGTGACCGG 
TTTCGCTGCA TGATTGTCGA TGGCTGGTGG ATGGGTCAGA TTGAATCGCG CCACGCCCTC 
TCTGCTGACT TCCCCGACTC GTCCTTCATG TGTTTCCGCG TGCGCTGGGA TAATGGAGAG 
TATGAGTTTA TGAGTCCCTG GGACATGGAA CCCATCGATC AGCAAAGAGT ACCGGACGAG 
GTGGGAGGTG CTGTTCCAGT TCTACCGGAG GAGATTCGCG CCACTCTATA CCAGCCCAAG 
TCCGAGGAAT GGCACAGAGG GGATCGCGAT GGCACCTGCA GGCGAATTAT CAATGGATTG 
GAGCAGGTTA TGCGTCTCTC TATCGCAGAG CATTTCCTGG CCCCCGTGGA CCTTAACATA 
TATCCAGACT ACGCTTATTT GATCGAATAT CCCATTGACC TGACCACAAT TAAATCTCGC 
TTTGAGAATC ACTTCTACCG CAGAATAACC TCAGCCCAGT TCGATGTACG CTACCTAGCC 
ACCAATGCGG AGCAGTACAA TCGCCGACAC GCAATTATCG TCAAGCATTC GCGAATTGTG 
ACAGATCTTT GTTTGAGGAT CATCAGGGAA CCGGATGAGA TAGATGTGGC TGCTGTTTAC 
CATCAGCTTG CGGATGTGTA CCATTCGTCC GAGTCGGAGA ACGAGGTAGA TTCGGATGTA 
GTGCCATCGA CCAGCACAGG GCCAACTACT TCAGCAGCTG CAGCAGCAGC TAGGCAAAGA 
GTATCCTCAG CCAGGCGATC CACTCGAATT CACTCGGAAG GAGACTGGCG TGCCGATTGT 
CGTCAGCTGC TTGATCTTAT GTGGGCGAGG ACAGATTCGG CGCCGTTCCG CGAGCCCGTT 
GACACCATCG ACTTCCCTGA CTACCTGGAG ATCATAGCTA CTCCAATGGA TCTGCGCACT 
GTAAAGGAGG ATCTGCTGGG CGGCAACTAC GATGATCCGT TGGACTTTGC CAAGGATGTG 
CGGCTGATCT TCCAAAACTC CAAGAACTAC AACACCAACA AGCGCTCACG GATTTATGCC 
ATGACCTTGC GACTCAGTGC GCTATTTGAG TCGCATATCA AGACAGTGGT CACCAGCTGG 
AAGGCGGCAT TAAGGCGCGC TAATAAAAAC AAGTCCGGCA GCAGTGGAGG AGGACGCGGT 
GGTTCCAGCA GCAGTCCCAA CAAGCGAGTC AAAAAGGAGC GATCCACGCG CCGTAATCAA 
AAGCCAACAG CAGCGGCCAG AAGATTGCCA GTCGCCAACT CCAGTGAAGA TGAGGACGAG 
GAGGATGATG ATGATGACGA CGATGATGAT GATGAGGAAG AGGCATCAAC GAGGGGGCGC 
TCTCGTCGGC GAAACGGTGT GGCCGTAAGT GCTAGTTCCT CTGGCTCCAA TCGCGTGACT 
TCTTCATCCG CCGCCCAACA GCGTCGAGGC AAAAGCAGCA GTAGCGAGGA GGAAGAGGAG 
GAGGAAACCA GTGCGCAGGC CACGTCGGAT GCCAGCAGCG ATGAAGAGGA AAGAGCCCAA 
TCCAATGACA GTTCGCCCCA GCAGACACGC ACGCGCAGGG CAAGGCAGCG GCAACGGCTG 
AGCGATGACT CCAATATCAA CGATTCCGAG GATAGTTACA ACCCCAACGA GGGACGCGGC 
AGTCGGCGAC GTCCAGGTGC CGCACGCCCG TCCAGCAATA GGCAGACAAA TGGCCACTCC 
AGCAAGAGGC GACGTTTGAA CAAAAGTCCA GCCAATGCTG CACCTGGCCC TAGTTTGCCA 
CGTCGGACAC GTCGCATAGC AGGCAGCTCC GCAGAAGAGG CTGCATCTCA CTCACAACAC 
GATGAGAGCA CACAGGATAG CCAGATAGGA CGTCGAAAGG GACGCTCGGC AAGCAGCAGG 
TCAATTGGCA ATGGACCAAG TACCAGTGCC GCAGCTGCCG CCAACTCCAG CCTTGAAAGT 
CCGTCACGGA ATACTCGAGC CAACGGAACC AGGACCTCTT CGCATCTTGG AGCCGAAAAT 
GATCACAGTT ACCACCTGCC AGTGCGCAAC GGGCGCATTA TCGAGTCCGA TACGGACTCT 
CCTGTCATCA GACCCACGCG ACAGAGCGCC AAGAGGGCGC TAATGGACTG GGGTCGTGGA 
AGCGATATTG AAGAGGAGGA GGAGGAAGAG GAAGAAGACA GCGAGGAAGC CGAGGAGATA 
CTGCCAACAC CTACAAAGGA CGACTTGCCC TCTACCAGCC GAGCCGCTTT GGCTCAGGCG 
GCTGCTGGTC CTTCTCGCCA GCTACGCACG AACCGCAACA CCGTCGTTCG TCCAGCCCAG 
AGTGACGACG ATGACGACGA CGACAGTGAC AACGAGCCGC TGGCCAACAA TCAACAAAAA 
AATTATCAAT CACCTGCAAA ATCTAGCTCA ACCGCAACAG TGGCCCCTCA TCCACTGAGC 
CTGCGCCGTC GATTAGCCTC GCCGCCAGAA CGGAATACCC ATATGACCCG TTCCCATGCC 
ACCTCCACAA CGTCCTCGGC CGCAGCATCA CGGGCAGAAC AACAACCAGC GGTTTCCACC 
CACGATCACA ACTACCTAGG CAACGTGAAT TCATCGAGGG CACCGCGCCG CATTCTTTCA 
CGCCACCAAC GAAATGCCGA CGAGTTGGAT ACCAGTCTAG ATGTCCTGGA AAACTCGCGT 
CTGCTCTCAG CCATAGCCAA TCACCGGCGA CCCACCTCTA CCACGAACAG CAACAGTGCG 
AGCAACGTGG TTACAAGGCG TCTGCGTGGT CGCGCTAGCC AGGCGAATTC GCACGACGAG 
GATCATGAGG AAGAGGAGGC TGCTGCTTCC GATGAGGGCA CTGATGGAGG CTCTAGCTCG 
CAGAGCTACG AAGATGAGGA TGAAGACTCC GACTCGGAAG ACAATCAGCC GCTGACCAGC 
TACGTATCGC CCAGCAATGG TCGTCCGAGG CGAAAGGCAA AAACCTCCTC CTCCTCCGCA 
ACAACTCAGG AGCGTCCGCT GCGACGCACA CGTCGCCCGC CATCCAATAA CTCCATCGCG 
AACGACAACG ATGACGATGA TGACTATGTT GTGCCGCGGA GTCGTCGCGC CAACCGGGGT 
GGAAACCAGC GAGCCGGGCG CAACCAAAAG AGACCGCGCT ACAATGAGCA GTCCGAGGAG 
GAAGAGGCGG CGGCTGGATA TTCCCATCGG AGTAAACGAA TCCGTAACGG CGACGCTCAT 
GCCAGCTCTC AGGAGCGATC ACAGGCCTCC ACCGAGCGGG ACCAGGAGGA AGAAGAGGAC 
GGTACTTCGG TGGACTCGGA AGAACAGCAG GTGAACGTTA GCAGCAGAGG CAGGGTGCGC 
AAGCCGTCTG CCAAGGCGCG CGGTATCTTC AAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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BRWD3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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