Sec10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sec10
Entrez Gene ID	42863
Full Name	Secretory 10
Synonyms	CG6159,Dmel\CG6159,P71,Sec10p,dSec10,dsec10,sec10
General protein information	Preferred Names Secretory 10 Names CG6159 gene product from transcript CG6159-RA CG6159-PA P71 Sec10 ortholog Sec10-PA sec10 secretory 10
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95E-95E

mRNA and Protein(s)

mRNA	Protein	Name
NM_142961.5	NP_651218.1	secretory 10

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004147	XP_313031.3
Eremothecium gossypii	AGOS_AGL130C	NP_986537.2
Canis lupus familiaris (dog)	EXOC5	XP_547832.2
Danio rerio (zebrafish)	exoc5	NP_001107268.1
Oryza sativa (rice)	Os12g0238100	NP_001066469.1
Homo sapiens (human)	EXOC5	NP_006535.1
Pan troglodytes (chimpanzee)	EXOC5	XP_003314397.1
	EXOC5	XP_001090127.1
Bos taurus (cattle)	EXOC5	NP_001179640.1
Mus musculus (house mouse)	Exoc5	NP_997097.1
Rattus norvegicus (Norway rat)	Exoc5	NP_071540.1
Caenorhabditis elegans (roundworm)	sec-10	NP_501283.1
Schizosaccharomyces pombe (fission yeast)	sec10	NP_593654.1
Magnaporthe oryzae (rice blast fungus)	MGG_04559	XP_003710921.1
Xenopus tropicalis (tropical clawed frog)	exoc5	NP_001017342.1
Gallus gallus (chicken)	EXOC5	NP_001012607.1
Drosophila melanogaster (fruit fly)	sec10	NP_651218.1
Saccharomyces cerevisiae (baker's yeast)	SEC10	NP_013267.1
Kluyveromyces lactis	KLLA0D18260g	XP_453869.1
Arabidopsis thaliana (thale cress)	SEC10	NP_568270.3
Neurospora crassa	NCU09313	XP_963955.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dSec10 has no detectable role in most forms of polarized trafficking/exocytosis, including neurotransmission, but rather is essential for endocrine secretion
Title: Drosophila sec10 is required for hormone secretion but not general exocytosis or neurotransmission.

The following Sec10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sec10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05898	NM_142961.5 Latest version!	Drosophila melanogaster secretory 10 (Sec10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sec10 ( NM_142961.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651218.1 Drosophila melanogaster secretory 10 (Sec10), mRNA.

Price & Availability↑

CloneID	OFa05898
Clone ID Related Accession (Same CDS sequence)	NM_142961.5
Accession Version	NM_142961.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secretory 10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142961.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTATCCC AATACATGGA GGAGTTTGAA CAGGAACCCT TTGAGGTGGG CGAGTTCATA 
GAGCGTCTGA CTTGGCGCAC CAACAATGAA CTCCAGAACA GCGAGGACTT TCATCCTGTG 
GCCCTCCACG ATACCTTCAT CCAGACCATT AAGGACCTAA AGATCCTGCA GGAGAAGCAA 
CAAAGCAAGT GCGAACGGCT AGAGGAGTCA CTGCGCCAGG AGAAGGAGTC GCACGCCAAA 
AAGATTGCCA AGCTCCAAGA ACGCCACCAA ACGGCCATTG ATGTGTTCGG CCAGCTGGAC 
GAAAAAATCA ATTCGGTGGC CGGCAAGATC ATGCACCTGG GCGAACAGTT GGAGAATGTG 
AACACTCCAC GCAGTCGTTC AGTGGAGGCC CAGAAGTTAC TCAATTTTAT GTCCGAGTTT 
TTAGCCGCTG GCCCTGTGAT TGTCAACGAT ATTTTTGCGG ATGCCGCAAG ATTAAGTGAG 
GCCGCAGATG TGATACAAAA GCTCTACGCT ATCTCGCAGG ATCTGCCGCC CGGAAACTTT 
GCGGAATCCA AAAGAAAAAT CGAAAAGAAA TACGATGAAG TCGAGCGGCG GTTGATCGAA 
GAGTTTGCCA CCGCCCAGAA GAGCGAGGAC ATCGAGCGCA TGAAGACACT GGCCCAGATC 
TTGTCCCAGT TCAAGGGTTA CACTCAGTGT GTAGACGCAT ACATCGAGCA GAGCCAAATG 
CAACCGTACA GTGGCAAAGA CATATTTATA GGCATTGTAC CGCTATGCAA GCATCACTAC 
GAGATAATCC AGAAGGTGTT TGCCAATCCG CAGCAGGTTA TGTCCAAGTT CATACTTAAC 
ATATATCAAC TGAAGCTTCA CCAGTACGCC ATGACTAAGT TGGAGGACAA AAAAGACGAG 
GAGAAATATC TTCGCACCCT TTATGAGTTA TACTCGCGCA CACTAAAATT GTCAACAGAT 
CTTCAAATCT ACATGTCCAC GATCGACGAC GACTTGTTAC AGAAGCTGAC ACAGCAGATT 
TTTATAAAGC ATTTGGCCGG CTACGCCGAA ATGGAGACCA AATGCCTCAC AGCCAAGTGT 
TCCACAGAGC TGGAAAAGTT CTATGCCAGC AAGAAACACC AAAAGACTGC AACTACTAAG 
GGCTTTCGGA GAAACATGGA GGTGCTGATA GCCACGCGGG CTAACATAAA TATTGCTGCC 
ATCGAGGACT ATGGCGGGGA GACGTTCCTA TCCGAAGAGT TGGCCATCAA CATGCTGCAG 
GAGGCCAAGG CGTCGCTTAA GCGCTGTCGC CTGCTGTCCA ACGAGACCGA ACTACCAGGC 
AATGCTATAA AGCTAAACGA TATCCTTCTT CGTTTCTTGA TGCACGAGCA CGTAGATTAC 
GCACTGGAGT TGGGACTGCA AGCAGTGCCA CTGGCCGAGG GCAGGGTCTT TCCCCAGCTC 
TACTTCTTTG ATGTGGTGCA AAAGACGAAC ATCATTGTCC ATCTACTGGA CAAGCTGTGC 
CACACATCTG TCATACCCTG TGTGAGTAAT ACACCCAAGT ACTCGGACTA TGTGTTCAAA 
AAACGTATTC TGATGGAGCA AATCGAGACA AAGCTGGACC AGGGTCTTGA TCGCTCTATT 
AGCGCTGTTA TTGGCTGGGT CAAGGTATAT TTGCAATATG AACAAAAGAA AACGGACTAC 
AAGCCGGAAA CAGATGTGGA TACAATATCT TCAGCGGCCT GCTTACAAGT CGTTCAGAAT 
CTGCAGCCCG TGATTGTGCA GATTAAAAAA TGTGTTGATG GAGAGAATTT GCAGAATGTT 
CTTACAGAAT TTGGAACTCG GTTGCACCGA GTAATCTACG ATCACCTGCA GACCATGCAG 
TTCAACACGG CTGGCGCCAT GTGTGCCATC TGCGACGTGA ACGAGTATCG CAAGTGCATT 
CGCGAGCTGG ATAGTCCACT GGTCACGCAG CTGTTTGACA TACTGCATGC ATTGTGCAAT 
TTACTACTTG TTAAGCCCCA AAACCTTCAA GAAGTTTGCA CGGGTGACAC TCTGAATTAT 
CTGGACAAGT CGGTGGTGCG GCAATTCATT CAGCTGCGCA CTGATTTCAG GATCATCAAG 
AACACCAACT ATCTGAAGGG TATTATTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651218.1
CDS	84..2216
Translation	MLSQYMEEFEQEPFEVGEFIERLTWRTNNELQNSEDFHPVALHDTFIQTIKDLKILQEKQQSKCERLEESLRQEKESHAKKIAKLQERHQTAIDVFGQLDEKINSVAGKIMHLGEQLENVNTPRSRSVEAQKLLNFMSEFLAAGPVIVNDIFADAARLSEAADVIQKLYAISQDLPPGNFAESKRKIEKKYDEVERRLIEEFATAQKSEDIERMKTLAQILSQFKGYTQCVDAYIEQSQMQPYSGKDIFIGIVPLCKHHYEIIQKVFANPQQVMSKFILNIYQLKLHQYAMTKLEDKKDEEKYLRTLYELYSRTLKLSTDLQIYMSTIDDDLLQKLTQQIFIKHLAGYAEMETKCLTAKCSTELEKFYASKKHQKTATTKGFRRNMEVLIATRANINIAAIEDYGGETFLSEELAINMLQEAKASLKRCRLLSNETELPGNAIKLNDILLRFLMHEHVDYALELGLQAVPLAEGRVFPQLYFFDVVQKTNIIVHLLDKLCHTSVIPCVSNTPKYSDYVFKKRILMEQIETKLDQGLDRSISAVIGWVKVYLQYEQKKTDYKPETDVDTISSAACLQVVQNLQPVIVQIKKCVDGENLQNVLTEFGTRLHRVIYDHLQTMQFNTAGAMCAICDVNEYRKCIRELDSPLVTQLFDILHALCNLLLVKPQNLQEVCTGDTLNYLDKSVVRQFIQLRTDFRIIKNTNYLKGIIE

Target ORF information:

RefSeq Version	NM_142961.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secretory 10 (Sec10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142961.5

ORF Insert Sequence:

ATGCTATCCC AATACATGGA GGAGTTTGAA CAGGAACCCT TTGAGGTGGG CGAGTTCATA 
GAGCGTCTGA CTTGGCGCAC CAACAATGAA CTCCAGAACA GCGAGGACTT TCATCCTGTG 
GCCCTCCACG ATACCTTCAT CCAGACCATT AAGGACCTAA AGATCCTGCA GGAGAAGCAA 
CAAAGCAAGT GCGAACGGCT AGAGGAGTCA CTGCGCCAGG AGAAGGAGTC GCACGCCAAA 
AAGATTGCCA AGCTCCAAGA ACGCCACCAA ACGGCCATTG ATGTGTTCGG CCAGCTGGAC 
GAAAAAATCA ATTCGGTGGC CGGCAAGATC ATGCACCTGG GCGAACAGTT GGAGAATGTG 
AACACTCCAC GCAGTCGTTC AGTGGAGGCC CAGAAGTTAC TCAATTTTAT GTCCGAGTTT 
TTAGCCGCTG GCCCTGTGAT TGTCAACGAT ATTTTTGCGG ATGCCGCAAG ATTAAGTGAG 
GCCGCAGATG TGATACAAAA GCTCTACGCT ATCTCGCAGG ATCTGCCGCC CGGAAACTTT 
GCGGAATCCA AAAGAAAAAT CGAAAAGAAA TACGATGAAG TCGAGCGGCG GTTGATCGAA 
GAGTTTGCCA CCGCCCAGAA GAGCGAGGAC ATCGAGCGCA TGAAGACACT GGCCCAGATC 
TTGTCCCAGT TCAAGGGTTA CACTCAGTGT GTAGACGCAT ACATCGAGCA GAGCCAAATG 
CAACCGTACA GTGGCAAAGA CATATTTATA GGCATTGTAC CGCTATGCAA GCATCACTAC 
GAGATAATCC AGAAGGTGTT TGCCAATCCG CAGCAGGTTA TGTCCAAGTT CATACTTAAC 
ATATATCAAC TGAAGCTTCA CCAGTACGCC ATGACTAAGT TGGAGGACAA AAAAGACGAG 
GAGAAATATC TTCGCACCCT TTATGAGTTA TACTCGCGCA CACTAAAATT GTCAACAGAT 
CTTCAAATCT ACATGTCCAC GATCGACGAC GACTTGTTAC AGAAGCTGAC ACAGCAGATT 
TTTATAAAGC ATTTGGCCGG CTACGCCGAA ATGGAGACCA AATGCCTCAC AGCCAAGTGT 
TCCACAGAGC TGGAAAAGTT CTATGCCAGC AAGAAACACC AAAAGACTGC AACTACTAAG 
GGCTTTCGGA GAAACATGGA GGTGCTGATA GCCACGCGGG CTAACATAAA TATTGCTGCC 
ATCGAGGACT ATGGCGGGGA GACGTTCCTA TCCGAAGAGT TGGCCATCAA CATGCTGCAG 
GAGGCCAAGG CGTCGCTTAA GCGCTGTCGC CTGCTGTCCA ACGAGACCGA ACTACCAGGC 
AATGCTATAA AGCTAAACGA TATCCTTCTT CGTTTCTTGA TGCACGAGCA CGTAGATTAC 
GCACTGGAGT TGGGACTGCA AGCAGTGCCA CTGGCCGAGG GCAGGGTCTT TCCCCAGCTC 
TACTTCTTTG ATGTGGTGCA AAAGACGAAC ATCATTGTCC ATCTACTGGA CAAGCTGTGC 
CACACATCTG TCATACCCTG TGTGAGTAAT ACACCCAAGT ACTCGGACTA TGTGTTCAAA 
AAACGTATTC TGATGGAGCA AATCGAGACA AAGCTGGACC AGGGTCTTGA TCGCTCTATT 
AGCGCTGTTA TTGGCTGGGT CAAGGTATAT TTGCAATATG AACAAAAGAA AACGGACTAC 
AAGCCGGAAA CAGATGTGGA TACAATATCT TCAGCGGCCT GCTTACAAGT CGTTCAGAAT 
CTGCAGCCCG TGATTGTGCA GATTAAAAAA TGTGTTGATG GAGAGAATTT GCAGAATGTT 
CTTACAGAAT TTGGAACTCG GTTGCACCGA GTAATCTACG ATCACCTGCA GACCATGCAG 
TTCAACACGG CTGGCGCCAT GTGTGCCATC TGCGACGTGA ACGAGTATCG CAAGTGCATT 
CGCGAGCTGG ATAGTCCACT GGTCACGCAG CTGTTTGACA TACTGCATGC ATTGTGCAAT 
TTACTACTTG TTAAGCCCCA AAACCTTCAA GAAGTTTGCA CGGGTGACAC TCTGAATTAT 
CTGGACAAGT CGGTGGTGCG GCAATTCATT CAGCTGCGCA CTGATTTCAG GATCATCAAG 
AACACCAACT ATCTGAAGGG TATTATTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sec10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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