Lsd-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lsd-1
Entrez Gene ID	42810
Full Name	Lipid storage droplet-1
Synonyms	CG10374,DmPLIN1,Dmel\CG10374,LSD-1,LSDP1,Lsd1,Lsdp1,PLIN1,lsd1,plin1
General protein information	Preferred Names Lipid storage droplet-1 Names CG10374 gene product from transcript CG10374-RC CG10374-PA CG10374-PB CG10374-PC CG10374-PD Lsd-1-PA Lsd-1-PB Lsd-1-PC Lsd-1-PD lipid storage droplet protein 1 lipid storage droplet-1 lipid storage droplets surface binding protein perilipin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95B1-95B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_170092.3	NP_732904.2	lipid storage droplet-1, isoform A
NM_170093.3	NP_732905.2	lipid storage droplet-1, isoform B
NM_142926.4	NP_651183.3	lipid storage droplet-1, isoform C
NM_001275954.1	NP_001262883.1	lipid storage droplet-1, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Lsd-1	NP_001262883.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002890	XP_003436284.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of the lipid storage droplet 1 gene in the entire Drosophila melanogaster body caused lethality, indicating that Lsd1 is an essential function for viability.
Title: Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster.

reductions in the expression of the Pumilio translational repressor complex enhanced phenotypes due to dLsd1 depletion in Drosophila. the PUM complex is a target of LSD1 regulation.
Title: The LSD1 Family of Histone Demethylases and the Pumilio Posttranscriptional Repressor Function in a Complex Regulatory Feedback Loop.

Lsd1 restricts the number of GSC-like cells by regulating a diverse group of genes and provide further evidence that escort cell function must be carefully controlled during development and adulthood to ensure proper germline differentiation.
Title: Lsd1 restricts the number of germline stem cells by regulating multiple targets in escort cells.

SIRT1, in complex with LSD1, has a role in developmental epigenetic gene repression and regulation.
Title: A SIRT1-LSD1 corepressor complex regulates Notch target gene expression and development.

Phosphorylation of Lsd1 promotes a 1.7-fold activation of the main fat body lipase and demonstrates the direct link between Lsd1 phosphorylation and activation of lipolysis.
Title: Function and structure of lipid storage droplet protein 1 studied in lipoprotein complexes.

The following Lsd-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lsd-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05832	NM_142926.4 Latest version!	Drosophila melanogaster lipid storage droplet-1, transcript variant C (Lsd-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa05832	NM_001275954.1 Latest version!	Drosophila melanogaster lipid storage droplet-1, transcript variant D (Lsd-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa05833	NM_170093.3 Latest version!	Drosophila melanogaster lipid storage droplet-1, transcript variant B (Lsd-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa05834	NM_170092.3 Latest version!	Drosophila melanogaster lipid storage droplet-1, transcript variant A (Lsd-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lsd-1 ( NM_142926.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651183.3 Drosophila melanogaster lipid storage droplet-1, transcript variant C (Lsd-1), mRNA.

Price & Availability↑

CloneID	OFa05832
Clone ID Related Accession (Same CDS sequence)	NM_142926.4 , NM_001275954.1
Accession Version	NM_142926.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lipid storage droplet-1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142926.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAACTG CAACCAGCGG CAGTGGACTC CATCTGGAGG CCATCGATCG CATCGGCTCC 
ATTCCCCTTG TGGAATCCAG TGTGAAGCGG GTGGAGACCA TCTACGATAA GGTGAAGAAC 
AACAATAGGC TATTCTCATG GTATTTTGAG ACGGCGGAGG CCACCATTTC GGCCGCCTAC 
GAAACCATCC AGCCGGCGGT CAAGCTCTTC GAGCCATCCA TTCAGCGCCT GGACAACGTG 
ATGTGCAAAA GCCTGGACAT TCTGGAGCAG CGCATACCAC TGGTCTATCT GCCGCCCGAA 
ATGATGTACT GGAACACCAA GGAGTACATG TCCGATCACC TGGTGCGTCC GGTTCTGAAA 
CGCGCCGATT CTGTCAAGCA AATCGGTAAT GCTGTCCTCG AGAGTCCACT TACCACTTAT 
GCAGCCGAGC GCATTGATGG CGCTTTCACA GTCGGCGATA AGTTCGTGGA CAAGTACCTG 
GTGCCCATCC AAACAGATCA GGATCAGACC GATGGCCCAC AGGAGGATGA TAACGAGGCC 
GTGCCGGACG AGAGGGGCGC CATCAAGGCG ATCCATCATG GTCAACGCTT CTCCCGCAAA 
CTGAAGCGCC GCCTTACCCA AAGAACCATC GCAGAGGCTC GCGCCCTCAA AAAGCAGAGC 
AAGGAGGCGA TCCACGTGCT CTTCTATGCC GCAGAATTGA TTGCCACCGA TCCCAAGCAG 
GCCGTTCAAA AGGCCAAGGA ACTGTGGGTC TATCTCAGTG CAGATGAACC CGAAAATCAA 
GCGAGGCCCG CCACTCTGGA GCAGTTGATT GTGCTACTGA CCCGCGAATC GGCTAGGCGT 
GTGGTTCATC TAGTGAACTT CAGTGCTCAT GTGGCAGCCA ACATACCCAG AAACCTGGCG 
CACACGACAA CAGAGGTTGC CCACCATATT ATCTATATCA ACCACCGCAT CATCACAATC 
TCACGGCTGG ACAAGGTTAA AACCATCTCC AAAGAGGAAG CTGAATCTCT TTTCAAGCGC 
ATGCTGGCGT TCTATGGTAG CCTTCAGGGC CTGACCAATG CCTACCTGGA GCGCGTGGCC 
AGCTTTCTAT CCGGACGCAT GGAGGCCGAG AAGGTGACGG GCAGCGATGG CGGCAATTCC 
AACCACCGGT CATCGCGGAG GAGGCAGGAT CCGAACCATT ATTCAGCCAC CCACAATAAC 
ATCAACGGCG TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651183.3
CDS	457..1674
Translation	MATATSGSGLHLEAIDRIGSIPLVESSVKRVETIYDKVKNNNRLFSWYFETAEATISAAYETIQPAVKLFEPSIQRLDNVMCKSLDILEQRIPLVYLPPEMMYWNTKEYMSDHLVRPVLKRADSVKQIGNAVLESPLTTYAAERIDGAFTVGDKFVDKYLVPIQTDQDQTDGPQEDDNEAVPDERGAIKAIHHGQRFSRKLKRRLTQRTIAEARALKKQSKEAIHVLFYAAELIATDPKQAVQKAKELWVYLSADEPENQARPATLEQLIVLLTRESARRVVHLVNFSAHVAANIPRNLAHTTTEVAHHIIYINHRIITISRLDKVKTISKEEAESLFKRMLAFYGSLQGLTNAYLERVASFLSGRMEAEKVTGSDGGNSNHRSSRRRQDPNHYSATHNNINGVY

Target ORF information:

RefSeq Version	NM_142926.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lipid storage droplet-1, transcript variant C (Lsd-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142926.4

ORF Insert Sequence:

ATGGCAACTG CAACCAGCGG CAGTGGACTC CATCTGGAGG CCATCGATCG CATCGGCTCC 
ATTCCCCTTG TGGAATCCAG TGTGAAGCGG GTGGAGACCA TCTACGATAA GGTGAAGAAC 
AACAATAGGC TATTCTCATG GTATTTTGAG ACGGCGGAGG CCACCATTTC GGCCGCCTAC 
GAAACCATCC AGCCGGCGGT CAAGCTCTTC GAGCCATCCA TTCAGCGCCT GGACAACGTG 
ATGTGCAAAA GCCTGGACAT TCTGGAGCAG CGCATACCAC TGGTCTATCT GCCGCCCGAA 
ATGATGTACT GGAACACCAA GGAGTACATG TCCGATCACC TGGTGCGTCC GGTTCTGAAA 
CGCGCCGATT CTGTCAAGCA AATCGGTAAT GCTGTCCTCG AGAGTCCACT TACCACTTAT 
GCAGCCGAGC GCATTGATGG CGCTTTCACA GTCGGCGATA AGTTCGTGGA CAAGTACCTG 
GTGCCCATCC AAACAGATCA GGATCAGACC GATGGCCCAC AGGAGGATGA TAACGAGGCC 
GTGCCGGACG AGAGGGGCGC CATCAAGGCG ATCCATCATG GTCAACGCTT CTCCCGCAAA 
CTGAAGCGCC GCCTTACCCA AAGAACCATC GCAGAGGCTC GCGCCCTCAA AAAGCAGAGC 
AAGGAGGCGA TCCACGTGCT CTTCTATGCC GCAGAATTGA TTGCCACCGA TCCCAAGCAG 
GCCGTTCAAA AGGCCAAGGA ACTGTGGGTC TATCTCAGTG CAGATGAACC CGAAAATCAA 
GCGAGGCCCG CCACTCTGGA GCAGTTGATT GTGCTACTGA CCCGCGAATC GGCTAGGCGT 
GTGGTTCATC TAGTGAACTT CAGTGCTCAT GTGGCAGCCA ACATACCCAG AAACCTGGCG 
CACACGACAA CAGAGGTTGC CCACCATATT ATCTATATCA ACCACCGCAT CATCACAATC 
TCACGGCTGG ACAAGGTTAA AACCATCTCC AAAGAGGAAG CTGAATCTCT TTTCAAGCGC 
ATGCTGGCGT TCTATGGTAG CCTTCAGGGC CTGACCAATG CCTACCTGGA GCGCGTGGCC 
AGCTTTCTAT CCGGACGCAT GGAGGCCGAG AAGGTGACGG GCAGCGATGG CGGCAATTCC 
AACCACCGGT CATCGCGGAG GAGGCAGGAT CCGAACCATT ATTCAGCCAC CCACAATAAC 
ATCAACGGCG TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lsd-1 ( NM_001275954.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262883.1 Drosophila melanogaster lipid storage droplet-1, transcript variant D (Lsd-1), mRNA.

Price & Availability↑

CloneID	OFa05832
Clone ID Related Accession (Same CDS sequence)	NM_142926.4 , NM_001275954.1
Accession Version	NM_001275954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lipid storage droplet-1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAACTG CAACCAGCGG CAGTGGACTC CATCTGGAGG CCATCGATCG CATCGGCTCC 
ATTCCCCTTG TGGAATCCAG TGTGAAGCGG GTGGAGACCA TCTACGATAA GGTGAAGAAC 
AACAATAGGC TATTCTCATG GTATTTTGAG ACGGCGGAGG CCACCATTTC GGCCGCCTAC 
GAAACCATCC AGCCGGCGGT CAAGCTCTTC GAGCCATCCA TTCAGCGCCT GGACAACGTG 
ATGTGCAAAA GCCTGGACAT TCTGGAGCAG CGCATACCAC TGGTCTATCT GCCGCCCGAA 
ATGATGTACT GGAACACCAA GGAGTACATG TCCGATCACC TGGTGCGTCC GGTTCTGAAA 
CGCGCCGATT CTGTCAAGCA AATCGGTAAT GCTGTCCTCG AGAGTCCACT TACCACTTAT 
GCAGCCGAGC GCATTGATGG CGCTTTCACA GTCGGCGATA AGTTCGTGGA CAAGTACCTG 
GTGCCCATCC AAACAGATCA GGATCAGACC GATGGCCCAC AGGAGGATGA TAACGAGGCC 
GTGCCGGACG AGAGGGGCGC CATCAAGGCG ATCCATCATG GTCAACGCTT CTCCCGCAAA 
CTGAAGCGCC GCCTTACCCA AAGAACCATC GCAGAGGCTC GCGCCCTCAA AAAGCAGAGC 
AAGGAGGCGA TCCACGTGCT CTTCTATGCC GCAGAATTGA TTGCCACCGA TCCCAAGCAG 
GCCGTTCAAA AGGCCAAGGA ACTGTGGGTC TATCTCAGTG CAGATGAACC CGAAAATCAA 
GCGAGGCCCG CCACTCTGGA GCAGTTGATT GTGCTACTGA CCCGCGAATC GGCTAGGCGT 
GTGGTTCATC TAGTGAACTT CAGTGCTCAT GTGGCAGCCA ACATACCCAG AAACCTGGCG 
CACACGACAA CAGAGGTTGC CCACCATATT ATCTATATCA ACCACCGCAT CATCACAATC 
TCACGGCTGG ACAAGGTTAA AACCATCTCC AAAGAGGAAG CTGAATCTCT TTTCAAGCGC 
ATGCTGGCGT TCTATGGTAG CCTTCAGGGC CTGACCAATG CCTACCTGGA GCGCGTGGCC 
AGCTTTCTAT CCGGACGCAT GGAGGCCGAG AAGGTGACGG GCAGCGATGG CGGCAATTCC 
AACCACCGGT CATCGCGGAG GAGGCAGGAT CCGAACCATT ATTCAGCCAC CCACAATAAC 
ATCAACGGCG TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262883.1
CDS	76..1293
Translation	MATATSGSGLHLEAIDRIGSIPLVESSVKRVETIYDKVKNNNRLFSWYFETAEATISAAYETIQPAVKLFEPSIQRLDNVMCKSLDILEQRIPLVYLPPEMMYWNTKEYMSDHLVRPVLKRADSVKQIGNAVLESPLTTYAAERIDGAFTVGDKFVDKYLVPIQTDQDQTDGPQEDDNEAVPDERGAIKAIHHGQRFSRKLKRRLTQRTIAEARALKKQSKEAIHVLFYAAELIATDPKQAVQKAKELWVYLSADEPENQARPATLEQLIVLLTRESARRVVHLVNFSAHVAANIPRNLAHTTTEVAHHIIYINHRIITISRLDKVKTISKEEAESLFKRMLAFYGSLQGLTNAYLERVASFLSGRMEAEKVTGSDGGNSNHRSSRRRQDPNHYSATHNNINGVY

Target ORF information:

RefSeq Version	NM_001275954.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lipid storage droplet-1, transcript variant D (Lsd-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275954.1

ORF Insert Sequence:

ATGGCAACTG CAACCAGCGG CAGTGGACTC CATCTGGAGG CCATCGATCG CATCGGCTCC 
ATTCCCCTTG TGGAATCCAG TGTGAAGCGG GTGGAGACCA TCTACGATAA GGTGAAGAAC 
AACAATAGGC TATTCTCATG GTATTTTGAG ACGGCGGAGG CCACCATTTC GGCCGCCTAC 
GAAACCATCC AGCCGGCGGT CAAGCTCTTC GAGCCATCCA TTCAGCGCCT GGACAACGTG 
ATGTGCAAAA GCCTGGACAT TCTGGAGCAG CGCATACCAC TGGTCTATCT GCCGCCCGAA 
ATGATGTACT GGAACACCAA GGAGTACATG TCCGATCACC TGGTGCGTCC GGTTCTGAAA 
CGCGCCGATT CTGTCAAGCA AATCGGTAAT GCTGTCCTCG AGAGTCCACT TACCACTTAT 
GCAGCCGAGC GCATTGATGG CGCTTTCACA GTCGGCGATA AGTTCGTGGA CAAGTACCTG 
GTGCCCATCC AAACAGATCA GGATCAGACC GATGGCCCAC AGGAGGATGA TAACGAGGCC 
GTGCCGGACG AGAGGGGCGC CATCAAGGCG ATCCATCATG GTCAACGCTT CTCCCGCAAA 
CTGAAGCGCC GCCTTACCCA AAGAACCATC GCAGAGGCTC GCGCCCTCAA AAAGCAGAGC 
AAGGAGGCGA TCCACGTGCT CTTCTATGCC GCAGAATTGA TTGCCACCGA TCCCAAGCAG 
GCCGTTCAAA AGGCCAAGGA ACTGTGGGTC TATCTCAGTG CAGATGAACC CGAAAATCAA 
GCGAGGCCCG CCACTCTGGA GCAGTTGATT GTGCTACTGA CCCGCGAATC GGCTAGGCGT 
GTGGTTCATC TAGTGAACTT CAGTGCTCAT GTGGCAGCCA ACATACCCAG AAACCTGGCG 
CACACGACAA CAGAGGTTGC CCACCATATT ATCTATATCA ACCACCGCAT CATCACAATC 
TCACGGCTGG ACAAGGTTAA AACCATCTCC AAAGAGGAAG CTGAATCTCT TTTCAAGCGC 
ATGCTGGCGT TCTATGGTAG CCTTCAGGGC CTGACCAATG CCTACCTGGA GCGCGTGGCC 
AGCTTTCTAT CCGGACGCAT GGAGGCCGAG AAGGTGACGG GCAGCGATGG CGGCAATTCC 
AACCACCGGT CATCGCGGAG GAGGCAGGAT CCGAACCATT ATTCAGCCAC CCACAATAAC 
ATCAACGGCG TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lsd-1 ( NM_170093.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732905.2 Drosophila melanogaster lipid storage droplet-1, transcript variant B (Lsd-1), mRNA.

Price & Availability↑

CloneID	OFa05833
Clone ID Related Accession (Same CDS sequence)	NM_170093.3
Accession Version	NM_170093.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lipid storage droplet-1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170093.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCAAAA GCCTGGACAT TCTGGAGCAG CGCATACCAC TGGTCTATCT GCCGCCCGAA 
ATGATGTACT GGAACACCAA GGAGTACATG TCCGATCACC TGGTGCGTCC GGTTCTGAAA 
CGCGCCGATT CTGTCAAGCA AATCGGTAAT GCTGTCCTCG AGAGTCCACT TACCACTTAT 
GCAGCCGAGC GCATTGATGG CGCTTTCACA GTCGGCGATA AGTTCGTGGA CAAGTACCTG 
GTGCCCATCC AAACAGATCA GGATCAGACC GATGGCCCAC AGGAGGATGA TAACGAGGCC 
GTGCCGGACG AGAGGGGCGC CATCAAGGCG ATCCATCATG GTCAACGCTT CTCCCGCAAA 
CTGAAGCGCC GCCTTACCCA AAGAACCATC GCAGAGGCTC GCGCCCTCAA AAAGCAGAGC 
AAGGAGGCGA TCCACGTGCT CTTCTATGCC GCAGAATTGA TTGCCACCGA TCCCAAGCAG 
GCCGTTCAAA AGGCCAAGGA ACTGTGGGTC TATCTCAGTG CAGATGAACC CGAAAATCAA 
GCGAGGCCCG CCACTCTGGA GCAGTTGATT GTGCTACTGA CCCGCGAATC GGCTAGGCGT 
GTGGTTCATC TAGTGAACTT CAGTGCTCAT GTGGCAGCCA ACATACCCAG AAACCTGGCG 
CACACGACAA CAGAGGTTGC CCACCATATT ATCTATATCA ACCACCGCAT CATCACAATC 
TCACGGCTGG ACAAGGTTAA AACCATCTCC AAAGAGGAAG CTGAATCTCT TTTCAAGCGC 
ATGCTGGCGT TCTATGGTAG CCTTCAGGGC CTGACCAATG CCTACCTGGA GCGCGTGGCC 
AGCTTTCTAT CCGGACGCAT GGAGGCCGAG AAGGTGACGG GCAGCGATGG CGGCAATTCC 
AACCACCGGT CATCGCGGAG GAGGCAGGAT CCGAACCATT ATTCAGCCAC CCACAATAAC 
ATCAACGGCG TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732905.2
CDS	385..1362
Translation	MCKSLDILEQRIPLVYLPPEMMYWNTKEYMSDHLVRPVLKRADSVKQIGNAVLESPLTTYAAERIDGAFTVGDKFVDKYLVPIQTDQDQTDGPQEDDNEAVPDERGAIKAIHHGQRFSRKLKRRLTQRTIAEARALKKQSKEAIHVLFYAAELIATDPKQAVQKAKELWVYLSADEPENQARPATLEQLIVLLTRESARRVVHLVNFSAHVAANIPRNLAHTTTEVAHHIIYINHRIITISRLDKVKTISKEEAESLFKRMLAFYGSLQGLTNAYLERVASFLSGRMEAEKVTGSDGGNSNHRSSRRRQDPNHYSATHNNINGVY

Target ORF information:

RefSeq Version	NM_170093.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lipid storage droplet-1, transcript variant B (Lsd-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170093.3

ORF Insert Sequence:

ATGTGCAAAA GCCTGGACAT TCTGGAGCAG CGCATACCAC TGGTCTATCT GCCGCCCGAA 
ATGATGTACT GGAACACCAA GGAGTACATG TCCGATCACC TGGTGCGTCC GGTTCTGAAA 
CGCGCCGATT CTGTCAAGCA AATCGGTAAT GCTGTCCTCG AGAGTCCACT TACCACTTAT 
GCAGCCGAGC GCATTGATGG CGCTTTCACA GTCGGCGATA AGTTCGTGGA CAAGTACCTG 
GTGCCCATCC AAACAGATCA GGATCAGACC GATGGCCCAC AGGAGGATGA TAACGAGGCC 
GTGCCGGACG AGAGGGGCGC CATCAAGGCG ATCCATCATG GTCAACGCTT CTCCCGCAAA 
CTGAAGCGCC GCCTTACCCA AAGAACCATC GCAGAGGCTC GCGCCCTCAA AAAGCAGAGC 
AAGGAGGCGA TCCACGTGCT CTTCTATGCC GCAGAATTGA TTGCCACCGA TCCCAAGCAG 
GCCGTTCAAA AGGCCAAGGA ACTGTGGGTC TATCTCAGTG CAGATGAACC CGAAAATCAA 
GCGAGGCCCG CCACTCTGGA GCAGTTGATT GTGCTACTGA CCCGCGAATC GGCTAGGCGT 
GTGGTTCATC TAGTGAACTT CAGTGCTCAT GTGGCAGCCA ACATACCCAG AAACCTGGCG 
CACACGACAA CAGAGGTTGC CCACCATATT ATCTATATCA ACCACCGCAT CATCACAATC 
TCACGGCTGG ACAAGGTTAA AACCATCTCC AAAGAGGAAG CTGAATCTCT TTTCAAGCGC 
ATGCTGGCGT TCTATGGTAG CCTTCAGGGC CTGACCAATG CCTACCTGGA GCGCGTGGCC 
AGCTTTCTAT CCGGACGCAT GGAGGCCGAG AAGGTGACGG GCAGCGATGG CGGCAATTCC 
AACCACCGGT CATCGCGGAG GAGGCAGGAT CCGAACCATT ATTCAGCCAC CCACAATAAC 
ATCAACGGCG TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lsd-1 ( NM_170092.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732904.2 Drosophila melanogaster lipid storage droplet-1, transcript variant A (Lsd-1), mRNA.

Price & Availability↑

CloneID	OFa05834
Clone ID Related Accession (Same CDS sequence)	NM_170092.3
Accession Version	NM_170092.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lipid storage droplet-1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170092.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAACTG CAACCAGCGG CAGTGGACTC CATCTGGAGG CCATCGATCG CATCGGCTCC 
ATTCCCCTTG TGGAATCCAG TGTGAAGCGG GTGGAGACCA TCTACGATAA GGTGAAGAAC 
AACAATAGGC TATTCTCATG GTATTTTGAG ACGGCGGAGG CCACCATTTC GGCCGCCTAC 
GAAACCATCC AGCCGGCGGT CAAGCTCTTC GAGCCATCCA TTCAGCGCCT GGACAACGTG 
ATGTGCAAAA GCCTGGACAT TCTGGAGCAG CGCATACCAC TGGTCTATCT GCCGCCCGAA 
ATGATGTACT GGAACACCAA GGAGTACATG TCCGATCACC TGGTGCGTCC GGTTCTGAAA 
CGCGCCGATT CTGTCAAGCA AATCGGTAAT GCTGTCCTCG AGAGTCCACT TACCACTTAT 
GCAGCCGAGC GCATTGATGG CGCTTTCACA GTCGGCGATA AGTTCGTGGA CAAGTACCTG 
GTGCCCATCC AAACAGATCA GGATCAGACC GATGTGAAAA AATTGGTTTC GGCCCTGAAT 
GATAACTTTT CGCCGATTCG ACAACAATTA AACCGAATGT TTGGCCACAA AAGCCCACAG 
GAGGATGATA ACGAGGCCGT GCCGGACGAG AGGGGCGCCA TCAAGGCGAT CCATCATGGT 
CAACGCTTCT CCCGCAAACT GAAGCGCCGC CTTACCCAAA GAACCATCGC AGAGGCTCGC 
GCCCTCAAAA AGCAGAGCAA GGAGGCGATC CACGTGCTCT TCTATGCCGC AGAATTGATT 
GCCACCGATC CCAAGCAGGC CGTTCAAAAG GCCAAGGAAC TGTGGGTCTA TCTCAGTGCA 
GATGAACCCG AAAATCAAGC GAGGCCCGCC ACTCTGGAGC AGTTGATTGT GCTACTGACC 
CGCGAATCGG CTAGGCGTGT GGTTCATCTA GTGAACTTCA GTGCTCATGT GGCAGCCAAC 
ATACCCAGAA ACCTGGCGCA CACGACAACA GAGGTTGCCC ACCATATTAT CTATATCAAC 
CACCGCATCA TCACAATCTC ACGGCTGGAC AAGGTTAAAA CCATCTCCAA AGAGGAAGCT 
GAATCTCTTT TCAAGCGCAT GCTGGCGTTC TATGGTAGCC TTCAGGGCCT GACCAATGCC 
TACCTGGAGC GCGTGGCCAG CTTTCTATCC GGACGCATGG AGGCCGAGAA GGTGACGGGC 
AGCGATGGCG GCAATTCCAA CCACCGGTCA TCGCGGAGGA GGCAGGATCC GAACCATTAT 
TCAGCCACCC ACAATAACAT CAACGGCGTC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732904.2
CDS	76..1371
Translation	MATATSGSGLHLEAIDRIGSIPLVESSVKRVETIYDKVKNNNRLFSWYFETAEATISAAYETIQPAVKLFEPSIQRLDNVMCKSLDILEQRIPLVYLPPEMMYWNTKEYMSDHLVRPVLKRADSVKQIGNAVLESPLTTYAAERIDGAFTVGDKFVDKYLVPIQTDQDQTDVKKLVSALNDNFSPIRQQLNRMFGHKSPQEDDNEAVPDERGAIKAIHHGQRFSRKLKRRLTQRTIAEARALKKQSKEAIHVLFYAAELIATDPKQAVQKAKELWVYLSADEPENQARPATLEQLIVLLTRESARRVVHLVNFSAHVAANIPRNLAHTTTEVAHHIIYINHRIITISRLDKVKTISKEEAESLFKRMLAFYGSLQGLTNAYLERVASFLSGRMEAEKVTGSDGGNSNHRSSRRRQDPNHYSATHNNINGVY

Target ORF information:

RefSeq Version	NM_170092.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lipid storage droplet-1, transcript variant A (Lsd-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170092.3

ORF Insert Sequence:

ATGGCAACTG CAACCAGCGG CAGTGGACTC CATCTGGAGG CCATCGATCG CATCGGCTCC 
ATTCCCCTTG TGGAATCCAG TGTGAAGCGG GTGGAGACCA TCTACGATAA GGTGAAGAAC 
AACAATAGGC TATTCTCATG GTATTTTGAG ACGGCGGAGG CCACCATTTC GGCCGCCTAC 
GAAACCATCC AGCCGGCGGT CAAGCTCTTC GAGCCATCCA TTCAGCGCCT GGACAACGTG 
ATGTGCAAAA GCCTGGACAT TCTGGAGCAG CGCATACCAC TGGTCTATCT GCCGCCCGAA 
ATGATGTACT GGAACACCAA GGAGTACATG TCCGATCACC TGGTGCGTCC GGTTCTGAAA 
CGCGCCGATT CTGTCAAGCA AATCGGTAAT GCTGTCCTCG AGAGTCCACT TACCACTTAT 
GCAGCCGAGC GCATTGATGG CGCTTTCACA GTCGGCGATA AGTTCGTGGA CAAGTACCTG 
GTGCCCATCC AAACAGATCA GGATCAGACC GATGTGAAAA AATTGGTTTC GGCCCTGAAT 
GATAACTTTT CGCCGATTCG ACAACAATTA AACCGAATGT TTGGCCACAA AAGCCCACAG 
GAGGATGATA ACGAGGCCGT GCCGGACGAG AGGGGCGCCA TCAAGGCGAT CCATCATGGT 
CAACGCTTCT CCCGCAAACT GAAGCGCCGC CTTACCCAAA GAACCATCGC AGAGGCTCGC 
GCCCTCAAAA AGCAGAGCAA GGAGGCGATC CACGTGCTCT TCTATGCCGC AGAATTGATT 
GCCACCGATC CCAAGCAGGC CGTTCAAAAG GCCAAGGAAC TGTGGGTCTA TCTCAGTGCA 
GATGAACCCG AAAATCAAGC GAGGCCCGCC ACTCTGGAGC AGTTGATTGT GCTACTGACC 
CGCGAATCGG CTAGGCGTGT GGTTCATCTA GTGAACTTCA GTGCTCATGT GGCAGCCAAC 
ATACCCAGAA ACCTGGCGCA CACGACAACA GAGGTTGCCC ACCATATTAT CTATATCAAC 
CACCGCATCA TCACAATCTC ACGGCTGGAC AAGGTTAAAA CCATCTCCAA AGAGGAAGCT 
GAATCTCTTT TCAAGCGCAT GCTGGCGTTC TATGGTAGCC TTCAGGGCCT GACCAATGCC 
TACCTGGAGC GCGTGGCCAG CTTTCTATCC GGACGCATGG AGGCCGAGAA GGTGACGGGC 
AGCGATGGCG GCAATTCCAA CCACCGGTCA TCGCGGAGGA GGCAGGATCC GAACCATTAT 
TCAGCCACCC ACAATAACAT CAACGGCGTC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Lsd-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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