wge cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wge
Entrez Gene ID	42687
Full Name	winged eye
Synonyms	CG17621,CG31151,CG4902,Dmel\CG31151
General protein information	Preferred Names winged eye Names CG31151 gene product from transcript CG31151-RF CG31151-PC CG31151-PD CG31151-PE CG31151-PF wge-PC wge-PD wge-PE wge-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94C2-94C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043281.2	NP_001036746.1	winged eye, isoform C
NM_170037.2	NP_732790.3	winged eye, isoform D
NM_001275918.1	NP_001262847.1	winged eye, isoform E
NM_170038.2	NP_732791.2	winged eye, isoform F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest that WGE is a component of specific nuclear foci called the histone locus body in Drosophila with a role in heterochromatin formation and transposon silencing.
Title: Organ identity specification factor WGE localizes to the histone locus body and regulates histone expression to ensure genomic stability in Drosophila.

The following wge gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wge cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05725	NM_001275918.1 Latest version!	Drosophila melanogaster winged eye (wge), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa08683	NM_170038.2 Latest version!	Drosophila melanogaster winged eye (wge), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa08684	NM_001043281.2 Latest version!	Drosophila melanogaster winged eye (wge), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa08685	NM_170037.2 Latest version!	Drosophila melanogaster winged eye (wge), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wge ( NM_001275918.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262847.1 Drosophila melanogaster winged eye (wge), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa05725
Clone ID Related Accession (Same CDS sequence)	NM_001275918.1
Accession Version	NM_001275918.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	winged eye, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACGT TTAATCCTGG CGCCAGCAGC AGTGCCGCCG ACGTTCTGAG CGCCACCACA 
GCAACGGCTA CTTTTCTGGT GCCCACCACT GCGGCAGTAT CGCATCCTGG TGCCCATACC 
GCCCATCTGC AGCTGGATCA ATTCGGAGGC TTCTCCGCGG CGACGGCCAC TCCCCTGCAG 
CACCATCACC ATCAGCAGAC CAACTCGTCG TACACTTTTG TGCAGATCAA GAGGGAACCC 
TGCCAGGTGT CCGAAATAAG CTCGAACAAT TGCCACCAGC AACAGCAGCA ACATCACCAA 
CACCACCAGC ACCAGGTGCA GCATCAGGGC CTGTCTTCCG CCTCGATGAC CGCCTCCTCC 
AAGACCATGT CCTCCTCCAC ACTGACCACT CTCGTCAAAA TAGAGGCTCC CTCGCCAAAG 
GTATCGGAAT TGGAAAAGTC CACAGGCAAC TCCGTTCCCA TTGGCATCGC CGTGGCCAGG 
AAACGCCCGC AAGAAGCTCT GGTGCCGGCT ATCAACACCC CCGCTACGCT GCCGCTGCAG 
CCACCACTCA ACAAGGACTT GAACTGCTTC GGCATACGCG TCGCTGACCT CGGTGCCACC 
AGTTGCGGCA ATTTGTACTT TACTGGCAAC GGCGACCTGA TGACCACCGG CACAGCCACC 
GCCGAGGAAC TGGCTCTGAG TGCAGCGGGT GTGAACCGGG CACCCTCCAC TTTCTGGCAG 
TATCCAAATG CATTACCCAT TGAATCAGTG ATTTCCATGT CGCCCGCCAC GGTGGGCCTG 
CAGTACTCGC GGGAGGCCAG TCGCGGACAG GTGGTGCTGC TGCCGGCCGG CCCAACAGCG 
CTCGCAGGCA TATCAAATTT TCCCCTCTTA ACGCCCACAG ATCCGTTCCA GCAGGCAGCG 
GCTGCGGCGG CCTTCGTCTG GCCCTCGGCC TACATCCCCC AGGCGCCGGC ACCTGGTGGT 
CACTCTGCTG CGGCAGCCGC CGCAGCTGCT TCGCTGCAGC CGACGCCCAA CTTGTCCAGT 
TTGCCCAACT TCATCTTTCC CTCGATGGGC GGCCACCAGG CGCTGAGCAC AACGCCTTCG 
TACGCGTCCA CCCTGCAGCT GTATCTGGCC GCTGCGGCCA CTGCGACGGG CAGCTCGACG 
ATGTGCCAGC ACAGCAATCA GCAGACCAGC ACCACAACCA CCAATTCCAC CATTAATCTG 
AGTCTGGCCG CTGGTTCGGG GGTCACGCAA AGCGGCAATG CGGCTAGCAG CTTAAGTTCC 
AGCAGTAGTC TCAGCGCGAG TAGCTCCCGT TTCCTCAGCC TGGCCACTGG TCAGACTGGG 
ATTCCTTCAC TTCTCTCCTT GCCGCCACCG ACAGGTTTGA AAGAGGAGTA CCCGATGCCG 
CTGGCATTGC CGCCCCTTGT GCCGCTCGAG GCGGCGCGGG ATAAGGAGCA GGCCTTGAAC 
CTAATGCGTC TGCCCACGCC GCCCACCTCG GCCACCATGG AGCCGTCTTC CCTAGGCCAT 
GCAACGCCCC ACCATCTCTT CCAGTCGGGT GCCATGGCTA CGCCCACGCC AGCATTGCTC 
AACCTATCGA TGCATGGCAA CGGTGGGGAG CTGCCTGCTG CAACGCCTCT GCCAGCGGTC 
AGTGATGAAG CGGCGCTGAA CTACAAGCTG CATGCACCGT TGACACCGCA GACACCTCCT 
CGCCTGGCGG ACATTCCTGT GTCGGGATCG ACTCAGCTCT TGCCGCAAAT GCAGGATGTC 
AATATACAGA CGGATACGCC CGTTTGCAGT GAAGACGAGA GCTTTCCGGG CTCAGCAAAG 
CCGCAGGATC CTGCTGCAGA AGCATTTCCG CCCCCAACCC ATGTCCAGCC GCTGGAACTA 
ACGAAGCCCA GTGAAGCCAG CCATACCCAG CCTACTGAGT GCCATACCCA GACCGAACCC 
AGTGACATAC CCAGTGCCAG CCAGGAGGAG TCAGCGGAGC AGACTCCCCC GGAACCCATA 
GAAACGATGA CGCAAGCCAC GCAGGCAGAT CAAACGTCCC CCGAGGACCT GACCGGTTTG 
GAGCTGCTCT CGAACATAAG CACCAATAGC AAACCACTGG TGCGAGTAAA ACAAGAGCCG 
GTGGAGCACA TAGAGCAGCC AGCGCCGCAG CCGCAGCCTG TGAATATAAT GCCCTCAGAG 
CCTACGCTGC CTATGCCGCC AATGCTGGAA ATGGAGCCCA CTTCAGAACG CGAACCACTT 
GGAGGTCTCA AGCTGCTGTG TGCACTCGCT GAGCAACGCA TCCAGGAGGA GGTGGTGCAG 
GGCAGCAGTC TGTTTGCTAC GCCGTCGTCG CGAACGCCCA CTCCCACGTC GCTGGCCCTG 
GGAACGACAG CTGCGACGCC GCCCATTTTC GAGGCCAAGT CCTGTTATGC CTTTGGGCAA 
TCGCAGGGTT CCGTCTTCCC CTCCTCAACA TCCTCCTTTC AGATGCCATT AAGCTCGCCC 
GGATTCCCCT CGATGCAGGG CATCGAGCTG CCAACCACGT CGGCTGGTAT CGTTGCCGAA 
CTGACTCCTG TGAAGCGCAA GAAGCACAAA CATTCCAAGT CCTCAGGCAG CGACAGCCGC 
AAGTCGGCAC GTTGCAGCAA GAAAAGCAAG AAGAAGCGCC ACCACAGCAG CAGCCGCCAG 
CAGTTCTCCG CTCCGGCCGA GGAGGATCTG GACCTGCAGG ACGATCAGCT GCAGTCGGAG 
CTACGCAATG CCCTCCACGC CGTGGATCCC AGCTATGCGC AGCGCTTTGG CCAGGAGGTG 
TTTAGCATCA TGGACAATAG TATGCGTATG CGACTGGCGA AAATAACGCG GCAGTATCGC 
AAGAAGAAAC GGAAGCTGGA TGAGATTTCC AAGCACAAAA AGAAGAAGAA GTGCTCCAAG 
CAGCAGCTCG AACTGCAACA GCAGCAGGCA TCGCAACAGG CGGTGCAGCC ATCTTCAGCT 
CTGCAACAGC CACAGATGAC GCTGTCCAGT GTCCTGGGAA CTTCATCCCC GTTGCGCGAT 
TACAAGTTCC CAAAGTTTTC GAGCAGTAAC CTTCAGGCGT CTAGTTTTTT GCGATTCCCG 
GACAAGACGC ACTCCTTCCC CCCACCGCCG TCCCTACAGC AATCCCAACC GGAGCCCAAT 
CCCTTGCCAA GCAGCAGCTC ACCGAGTACC TCGTCGTTTG TCCGCCTGGA ACCCAGCGCA 
TTGGACGCGG CCGTTGCCAT TGCACCAACT ACCTCTTCGA CTTCCACCTC CGGCTCACCA 
TCCACCAAGC AGGTGGCTTC CGCGCGGAAG CAGCGCAAGA TGAAGGCGAG CACCGTCGGC 
GCCGCTGGAG AGCAGACAGC GGCCACGGAG GCAAAGCGGC GATTTAGCGC CATTGATCGC 
GAGCTTCAGC TGACCAGCGA GCACCTGTAT CGCGATGAGA CACGTGTCCT CACGGACATG 
GGTGGTCTTT TCTATGCGGG TGTAATGAAG CCATTGCGGC CGCCAGATGT GTATTCAATC 
ACATTGGATG GAGAGCGTGG CAACAAGTCG CATGTAATGT CGCGCGAGGA TATACTCAAG 
GATACGATCC TCGAGGTAGC TCCGAAGAGC GTGGAGAGTG TACCAGTAGG CACGCGCCTG 
TGCGCCTACT GGAGTCAGCA GTACCGATGC CTTTATCCAG GACGTGCCAT CGACTCCGAG 
CAGGTCGTCG ATGGAACGGC CAGCAGCGCT ACGAATGCGG CTACAACAGC GCCGGACTTC 
GTAAGCGTAG AGTTCGACGA TGGCGACAGC GGCAGGATCC GCTTGCAGAA CATCCGTATG 
CTCCTCAGCG ATTATCCCAT AGCAGGCAAG CAGAAGCGAA GCGCTCTGCG CGGGGGTGAA 
AGTGGACCTG GCGGAGTCAC TCAGGACCAC TTGACCGTGC CCGGCTGCGA GGATTCGCAC 
AGCCATCATA GCCTGGGGAT GAGTAGCGAT AACACCACAT CGCTGGCGGC AACTATGGAG 
TTGTTCACGC AGCGCAGTGA GAAGAAGCGC CTGAAGAAGA GCCTCAAGAA GATGTCCAAA 
GCTCAAAATG GCGTTAGTCC GGCCACGGCA ACCAATGGTG GAGCTGATGC CGCTGCCTTG 
GGCGATGGTG TCAGTGCCGA AGACGCCGCT CGTAAGCACC ATAAGCACAA GAAACGCAAG 
AAGCACAAGA AGCATCACCG CAAGAATGGC AGCGAAGAGC AGGAGCAACA GGTAGTCCAG 
CAGGACTACT CTGCGGCAGG ACAGGAAGCG ACAGAGGCTC CTTCCACAGA GATGTCATCA 
GCGACCTCAA GTCGCGTTAA GGTGGAGGTC AAAGTGAAGA CAGAGCAGCT GGAGATGGAG 
GAGGAGGAGT CCGCATCCAA TCTCATGTCA GAGATATCCG ATGAGGCCAA GGGCGACGAT 
CTGGTCGAGC ATAACAACTC CAAGGGAAGC AGCAAGATTG CGGCCTTCCT GCCGGAACGG 
CAGCTATGGG GTTGGTATGG TACCGCCTAT CGCAAGGCGG GCGTCAAGGG ACGCGCCAGA 
AAGCAATTTT ACAAAACAAT CAAAAGGGGA AAGGAGACTA TCACGGTCGG GGACAGTGCA 
GTATTTCTAT CGACGGGCAG ACCAGATCGA CCCTATATCG GACGCATTGA GTCCATGTGG 
GAAACAACCA CGGGCAACAA GGTGGTGCGT GTGGCGTGGT TCTATCATCC GGAGGAGACG 
ACCGGCTGTC CAAAATTGAA ATTTCCGGGT GCTCTTTTTG AATCGCCGCA CGAGGATGAG 
AACGATGTGC AGACCATTTC GCATCGGTGT GAGGTGCTGC AGTTTGGCAG TTACTTCGAG 
AAGTTCGGTG CCGATTCGAA GCAATATCAA TCCATATACG ATAACAATGA TACATATTAT 
TTAGCCGGGC ACTATAACCC AAGGTTACAA GTGCTAAAAC TACAAGACGA TATTCCAACT 
CTCGAAGAGC TGCAGGATAC AAATACTACT ACTACTACTA CAGAAACTAC TACCGAGGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262847.1
CDS	628..5610
Translation	MATFNPGASSSAADVLSATTATATFLVPTTAAVSHPGAHTAHLQLDQFGGFSAATATPLQHHHHQQTNSSYTFVQIKREPCQVSEISSNNCHQQQQQHHQHHQHQVQHQGLSSASMTASSKTMSSSTLTTLVKIEAPSPKVSELEKSTGNSVPIGIAVARKRPQEALVPAINTPATLPLQPPLNKDLNCFGIRVADLGATSCGNLYFTGNGDLMTTGTATAEELALSAAGVNRAPSTFWQYPNALPIESVISMSPATVGLQYSREASRGQVVLLPAGPTALAGISNFPLLTPTDPFQQAAAAAAFVWPSAYIPQAPAPGGHSAAAAAAAASLQPTPNLSSLPNFIFPSMGGHQALSTTPSYASTLQLYLAAAATATGSSTMCQHSNQQTSTTTTNSTINLSLAAGSGVTQSGNAASSLSSSSSLSASSSRFLSLATGQTGIPSLLSLPPPTGLKEEYPMPLALPPLVPLEAARDKEQALNLMRLPTPPTSATMEPSSLGHATPHHLFQSGAMATPTPALLNLSMHGNGGELPAATPLPAVSDEAALNYKLHAPLTPQTPPRLADIPVSGSTQLLPQMQDVNIQTDTPVCSEDESFPGSAKPQDPAAEAFPPPTHVQPLELTKPSEASHTQPTECHTQTEPSDIPSASQEESAEQTPPEPIETMTQATQADQTSPEDLTGLELLSNISTNSKPLVRVKQEPVEHIEQPAPQPQPVNIMPSEPTLPMPPMLEMEPTSEREPLGGLKLLCALAEQRIQEEVVQGSSLFATPSSRTPTPTSLALGTTAATPPIFEAKSCYAFGQSQGSVFPSSTSSFQMPLSSPGFPSMQGIELPTTSAGIVAELTPVKRKKHKHSKSSGSDSRKSARCSKKSKKKRHHSSSRQQFSAPAEEDLDLQDDQLQSELRNALHAVDPSYAQRFGQEVFSIMDNSMRMRLAKITRQYRKKKRKLDEISKHKKKKKCSKQQLELQQQQASQQAVQPSSALQQPQMTLSSVLGTSSPLRDYKFPKFSSSNLQASSFLRFPDKTHSFPPPPSLQQSQPEPNPLPSSSSPSTSSFVRLEPSALDAAVAIAPTTSSTSTSGSPSTKQVASARKQRKMKASTVGAAGEQTAATEAKRRFSAIDRELQLTSEHLYRDETRVLTDMGGLFYAGVMKPLRPPDVYSITLDGERGNKSHVMSREDILKDTILEVAPKSVESVPVGTRLCAYWSQQYRCLYPGRAIDSEQVVDGTASSATNAATTAPDFVSVEFDDGDSGRIRLQNIRMLLSDYPIAGKQKRSALRGGESGPGGVTQDHLTVPGCEDSHSHHSLGMSSDNTTSLAATMELFTQRSEKKRLKKSLKKMSKAQNGVSPATATNGGADAAALGDGVSAEDAARKHHKHKKRKKHKKHHRKNGSEEQEQQVVQQDYSAAGQEATEAPSTEMSSATSSRVKVEVKVKTEQLEMEEEESASNLMSEISDEAKGDDLVEHNNSKGSSKIAAFLPERQLWGWYGTAYRKAGVKGRARKQFYKTIKRGKETITVGDSAVFLSTGRPDRPYIGRIESMWETTTGNKVVRVAWFYHPEETTGCPKLKFPGALFESPHEDENDVQTISHRCEVLQFGSYFEKFGADSKQYQSIYDNNDTYYLAGHYNPRLQVLKLQDDIPTLEELQDTNTTTTTTETTTED

Target ORF information:

RefSeq Version	NM_001275918.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster winged eye (wge), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275918.1

ORF Insert Sequence:

ATGGCCACGT TTAATCCTGG CGCCAGCAGC AGTGCCGCCG ACGTTCTGAG CGCCACCACA 
GCAACGGCTA CTTTTCTGGT GCCCACCACT GCGGCAGTAT CGCATCCTGG TGCCCATACC 
GCCCATCTGC AGCTGGATCA ATTCGGAGGC TTCTCCGCGG CGACGGCCAC TCCCCTGCAG 
CACCATCACC ATCAGCAGAC CAACTCGTCG TACACTTTTG TGCAGATCAA GAGGGAACCC 
TGCCAGGTGT CCGAAATAAG CTCGAACAAT TGCCACCAGC AACAGCAGCA ACATCACCAA 
CACCACCAGC ACCAGGTGCA GCATCAGGGC CTGTCTTCCG CCTCGATGAC CGCCTCCTCC 
AAGACCATGT CCTCCTCCAC ACTGACCACT CTCGTCAAAA TAGAGGCTCC CTCGCCAAAG 
GTATCGGAAT TGGAAAAGTC CACAGGCAAC TCCGTTCCCA TTGGCATCGC CGTGGCCAGG 
AAACGCCCGC AAGAAGCTCT GGTGCCGGCT ATCAACACCC CCGCTACGCT GCCGCTGCAG 
CCACCACTCA ACAAGGACTT GAACTGCTTC GGCATACGCG TCGCTGACCT CGGTGCCACC 
AGTTGCGGCA ATTTGTACTT TACTGGCAAC GGCGACCTGA TGACCACCGG CACAGCCACC 
GCCGAGGAAC TGGCTCTGAG TGCAGCGGGT GTGAACCGGG CACCCTCCAC TTTCTGGCAG 
TATCCAAATG CATTACCCAT TGAATCAGTG ATTTCCATGT CGCCCGCCAC GGTGGGCCTG 
CAGTACTCGC GGGAGGCCAG TCGCGGACAG GTGGTGCTGC TGCCGGCCGG CCCAACAGCG 
CTCGCAGGCA TATCAAATTT TCCCCTCTTA ACGCCCACAG ATCCGTTCCA GCAGGCAGCG 
GCTGCGGCGG CCTTCGTCTG GCCCTCGGCC TACATCCCCC AGGCGCCGGC ACCTGGTGGT 
CACTCTGCTG CGGCAGCCGC CGCAGCTGCT TCGCTGCAGC CGACGCCCAA CTTGTCCAGT 
TTGCCCAACT TCATCTTTCC CTCGATGGGC GGCCACCAGG CGCTGAGCAC AACGCCTTCG 
TACGCGTCCA CCCTGCAGCT GTATCTGGCC GCTGCGGCCA CTGCGACGGG CAGCTCGACG 
ATGTGCCAGC ACAGCAATCA GCAGACCAGC ACCACAACCA CCAATTCCAC CATTAATCTG 
AGTCTGGCCG CTGGTTCGGG GGTCACGCAA AGCGGCAATG CGGCTAGCAG CTTAAGTTCC 
AGCAGTAGTC TCAGCGCGAG TAGCTCCCGT TTCCTCAGCC TGGCCACTGG TCAGACTGGG 
ATTCCTTCAC TTCTCTCCTT GCCGCCACCG ACAGGTTTGA AAGAGGAGTA CCCGATGCCG 
CTGGCATTGC CGCCCCTTGT GCCGCTCGAG GCGGCGCGGG ATAAGGAGCA GGCCTTGAAC 
CTAATGCGTC TGCCCACGCC GCCCACCTCG GCCACCATGG AGCCGTCTTC CCTAGGCCAT 
GCAACGCCCC ACCATCTCTT CCAGTCGGGT GCCATGGCTA CGCCCACGCC AGCATTGCTC 
AACCTATCGA TGCATGGCAA CGGTGGGGAG CTGCCTGCTG CAACGCCTCT GCCAGCGGTC 
AGTGATGAAG CGGCGCTGAA CTACAAGCTG CATGCACCGT TGACACCGCA GACACCTCCT 
CGCCTGGCGG ACATTCCTGT GTCGGGATCG ACTCAGCTCT TGCCGCAAAT GCAGGATGTC 
AATATACAGA CGGATACGCC CGTTTGCAGT GAAGACGAGA GCTTTCCGGG CTCAGCAAAG 
CCGCAGGATC CTGCTGCAGA AGCATTTCCG CCCCCAACCC ATGTCCAGCC GCTGGAACTA 
ACGAAGCCCA GTGAAGCCAG CCATACCCAG CCTACTGAGT GCCATACCCA GACCGAACCC 
AGTGACATAC CCAGTGCCAG CCAGGAGGAG TCAGCGGAGC AGACTCCCCC GGAACCCATA 
GAAACGATGA CGCAAGCCAC GCAGGCAGAT CAAACGTCCC CCGAGGACCT GACCGGTTTG 
GAGCTGCTCT CGAACATAAG CACCAATAGC AAACCACTGG TGCGAGTAAA ACAAGAGCCG 
GTGGAGCACA TAGAGCAGCC AGCGCCGCAG CCGCAGCCTG TGAATATAAT GCCCTCAGAG 
CCTACGCTGC CTATGCCGCC AATGCTGGAA ATGGAGCCCA CTTCAGAACG CGAACCACTT 
GGAGGTCTCA AGCTGCTGTG TGCACTCGCT GAGCAACGCA TCCAGGAGGA GGTGGTGCAG 
GGCAGCAGTC TGTTTGCTAC GCCGTCGTCG CGAACGCCCA CTCCCACGTC GCTGGCCCTG 
GGAACGACAG CTGCGACGCC GCCCATTTTC GAGGCCAAGT CCTGTTATGC CTTTGGGCAA 
TCGCAGGGTT CCGTCTTCCC CTCCTCAACA TCCTCCTTTC AGATGCCATT AAGCTCGCCC 
GGATTCCCCT CGATGCAGGG CATCGAGCTG CCAACCACGT CGGCTGGTAT CGTTGCCGAA 
CTGACTCCTG TGAAGCGCAA GAAGCACAAA CATTCCAAGT CCTCAGGCAG CGACAGCCGC 
AAGTCGGCAC GTTGCAGCAA GAAAAGCAAG AAGAAGCGCC ACCACAGCAG CAGCCGCCAG 
CAGTTCTCCG CTCCGGCCGA GGAGGATCTG GACCTGCAGG ACGATCAGCT GCAGTCGGAG 
CTACGCAATG CCCTCCACGC CGTGGATCCC AGCTATGCGC AGCGCTTTGG CCAGGAGGTG 
TTTAGCATCA TGGACAATAG TATGCGTATG CGACTGGCGA AAATAACGCG GCAGTATCGC 
AAGAAGAAAC GGAAGCTGGA TGAGATTTCC AAGCACAAAA AGAAGAAGAA GTGCTCCAAG 
CAGCAGCTCG AACTGCAACA GCAGCAGGCA TCGCAACAGG CGGTGCAGCC ATCTTCAGCT 
CTGCAACAGC CACAGATGAC GCTGTCCAGT GTCCTGGGAA CTTCATCCCC GTTGCGCGAT 
TACAAGTTCC CAAAGTTTTC GAGCAGTAAC CTTCAGGCGT CTAGTTTTTT GCGATTCCCG 
GACAAGACGC ACTCCTTCCC CCCACCGCCG TCCCTACAGC AATCCCAACC GGAGCCCAAT 
CCCTTGCCAA GCAGCAGCTC ACCGAGTACC TCGTCGTTTG TCCGCCTGGA ACCCAGCGCA 
TTGGACGCGG CCGTTGCCAT TGCACCAACT ACCTCTTCGA CTTCCACCTC CGGCTCACCA 
TCCACCAAGC AGGTGGCTTC CGCGCGGAAG CAGCGCAAGA TGAAGGCGAG CACCGTCGGC 
GCCGCTGGAG AGCAGACAGC GGCCACGGAG GCAAAGCGGC GATTTAGCGC CATTGATCGC 
GAGCTTCAGC TGACCAGCGA GCACCTGTAT CGCGATGAGA CACGTGTCCT CACGGACATG 
GGTGGTCTTT TCTATGCGGG TGTAATGAAG CCATTGCGGC CGCCAGATGT GTATTCAATC 
ACATTGGATG GAGAGCGTGG CAACAAGTCG CATGTAATGT CGCGCGAGGA TATACTCAAG 
GATACGATCC TCGAGGTAGC TCCGAAGAGC GTGGAGAGTG TACCAGTAGG CACGCGCCTG 
TGCGCCTACT GGAGTCAGCA GTACCGATGC CTTTATCCAG GACGTGCCAT CGACTCCGAG 
CAGGTCGTCG ATGGAACGGC CAGCAGCGCT ACGAATGCGG CTACAACAGC GCCGGACTTC 
GTAAGCGTAG AGTTCGACGA TGGCGACAGC GGCAGGATCC GCTTGCAGAA CATCCGTATG 
CTCCTCAGCG ATTATCCCAT AGCAGGCAAG CAGAAGCGAA GCGCTCTGCG CGGGGGTGAA 
AGTGGACCTG GCGGAGTCAC TCAGGACCAC TTGACCGTGC CCGGCTGCGA GGATTCGCAC 
AGCCATCATA GCCTGGGGAT GAGTAGCGAT AACACCACAT CGCTGGCGGC AACTATGGAG 
TTGTTCACGC AGCGCAGTGA GAAGAAGCGC CTGAAGAAGA GCCTCAAGAA GATGTCCAAA 
GCTCAAAATG GCGTTAGTCC GGCCACGGCA ACCAATGGTG GAGCTGATGC CGCTGCCTTG 
GGCGATGGTG TCAGTGCCGA AGACGCCGCT CGTAAGCACC ATAAGCACAA GAAACGCAAG 
AAGCACAAGA AGCATCACCG CAAGAATGGC AGCGAAGAGC AGGAGCAACA GGTAGTCCAG 
CAGGACTACT CTGCGGCAGG ACAGGAAGCG ACAGAGGCTC CTTCCACAGA GATGTCATCA 
GCGACCTCAA GTCGCGTTAA GGTGGAGGTC AAAGTGAAGA CAGAGCAGCT GGAGATGGAG 
GAGGAGGAGT CCGCATCCAA TCTCATGTCA GAGATATCCG ATGAGGCCAA GGGCGACGAT 
CTGGTCGAGC ATAACAACTC CAAGGGAAGC AGCAAGATTG CGGCCTTCCT GCCGGAACGG 
CAGCTATGGG GTTGGTATGG TACCGCCTAT CGCAAGGCGG GCGTCAAGGG ACGCGCCAGA 
AAGCAATTTT ACAAAACAAT CAAAAGGGGA AAGGAGACTA TCACGGTCGG GGACAGTGCA 
GTATTTCTAT CGACGGGCAG ACCAGATCGA CCCTATATCG GACGCATTGA GTCCATGTGG 
GAAACAACCA CGGGCAACAA GGTGGTGCGT GTGGCGTGGT TCTATCATCC GGAGGAGACG 
ACCGGCTGTC CAAAATTGAA ATTTCCGGGT GCTCTTTTTG AATCGCCGCA CGAGGATGAG 
AACGATGTGC AGACCATTTC GCATCGGTGT GAGGTGCTGC AGTTTGGCAG TTACTTCGAG 
AAGTTCGGTG CCGATTCGAA GCAATATCAA TCCATATACG ATAACAATGA TACATATTAT 
TTAGCCGGGC ACTATAACCC AAGGTTACAA GTGCTAAAAC TACAAGACGA TATTCCAACT 
CTCGAAGAGC TGCAGGATAC AAATACTACT ACTACTACTA CAGAAACTAC TACCGAGGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wge ( NM_170038.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732791.2 Drosophila melanogaster winged eye (wge), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa08683
Clone ID Related Accession (Same CDS sequence)	NM_170038.2
Accession Version	NM_170038.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	winged eye, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170038.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACGT TTAATCCTGG CGCCAGCAGC AGTGCCGCCG ACGTTCTGAG CGCCACCACA 
GCAACGGCTA CTTTTCTGGT GCCCACCACT GCGGCAGTAT CGCATCCTGG TGCCCATACC 
GCCCATCTGC AGCTGGATCA ATTCGGAGGC TTCTCCGCGG CGACGGCCAC TCCCCTGCAG 
CACCATCACC ATCAGCAGAC CAACTCGTCG TACACTTTTG TGCAGATCAA GAGGGAACCC 
TGCCAGGTGT CCGAAATAAG CTCGAACAAT TGCCACCAGC AACAGCAGCA ACATCACCAA 
CACCACCAGC ACCAGGTGCA GCATCAGGGC CTGTCTTCCG CCTCGATGAC CGCCTCCTCC 
AAGACCATGT CCTCCTCCAC ACTGACCACT CTCGTCAAAA TAGAGGCTCC CTCGCCAAAG 
GTATCGGAAT TGGAAAAGTC CACAGGCAAC TCCGTTCCCA TTGGCATCGC CGTGGCCAGG 
AAACGCCCGC AAGAAGCTCT GGTGCCGGCT ATCAACACCC CCGCTACGCT GCCGCTGCAG 
CCACCACTCA ACAAGGACTT GAACTGCTTC GGCATACGCG TCGCTGACCT CGGTGCCACC 
AGTTGCGGCA ATTTGTACTT TACTGGCAAC GGCGACCTGA TGACCACCGG CACAGCCACC 
GCCGAGGAAC TGGCTCTGAG TGCAGCGGGT GTGAACCGGG CACCCTCCAC TTTCTGGCAG 
TATCCAAATC CGTTCCAGCA GGCAGCGGCT GCGGCGGCCT TCGTCTGGCC CTCGGCCTAC 
ATCCCCCAGG CGCCGGCACC TGGTGGTCAC TCTGCTGCGG CAGCCGCCGC AGCTGCTTCG 
CTGCAGCCGA CGCCCAACTT GTCCAGTTTG CCCAACTTCA TCTTTCCCTC GATGGGCGGC 
CACCAGGCGC TGAGCACAAC GCCTTCGTAC GCGTCCACCC TGCAGCTGTA TCTGGCCGCT 
GCGGCCACTG CGACGGGCAG CTCGACGATG TGCCAGCACA GCAATCAGCA GACCAGCACC 
ACAACCACCA ATTCCACCAT TAATCTGAGT CTGGCCGCTG GTTCGGGGGT CACGCAAAGC 
GGCAATGCGG CTAGCAGCTT AAGTTCCAGC AGTAGTCTCA GCGCGAGTAG CTCCCGTTTC 
CTCAGCCTGG CCACTGGTCA GACTGGGATT CCTTCACTTC TCTCCTTGCC GCCACCGACA 
GGTTTGAAAG AGGAGTACCC GATGCCGCTG GCATTGCCGC CCCTTGTGCC GCTCGAGGCG 
GCGCGGGATA AGGAGCAGGC CTTGAACCTA ATGCGTCTGC CCACGCCGCC CACCTCGGCC 
ACCATGGAGC CGTCTTCCCT AGGCCATGCA ACGCCCCACC ATCTCTTCCA GTCGGGTGCC 
ATGGCTACGC CCACGCCAGC ATTGCTCAAC CTATCGATGC ATGGCAACGG TGGGGAGCTG 
CCTGCTGCAA CGCCTCTGCC AGCGGTCAGT GATGAAGCGG CGCTGAACTA CAAGCTGCAT 
GCACCGTTGA CACCGCAGAC ACCTCCTCGC CTGGCGGACA TTCCTGTGTC GGGATCGACT 
CAGCTCTTGC CGCAAATGCA GGATGTCAAT ATACAGACGG ATACGCCCGT TTGCAGTGAA 
GACGAGAGCT TTCCGGGCTC AGCAAAGCCG CAGGATCCTG CTGCAGAAGC ATTTCCGCCC 
CCAACCCATG TCCAGCCGCT GGAACTAACG AAGCCCAGTG AAGCCAGCCA TACCCAGCCT 
ACTGAGTGCC ATACCCAGAC CGAACCCAGT GACATACCCA GTGCCAGCCA GGAGGAGTCA 
GCGGAGCAGA CTCCCCCGGA ACCCATAGAA ACGATGACGC AAGCCACGCA GGCAGATCAA 
ACGTCCCCCG AGGACCTGAC CGGTTTGGAG CTGCTCTCGA ACATAAGCAC CAATAGCAAA 
CCACTGGTGC GAGTAAAACA AGAGCCGGTG GAGCACATAG AGCAGCCAGC GCCGCAGCCG 
CAGCCTGTGA ATATAATGCC CTCAGAGCCT ACGCTGCCTA TGCCGCCAAT GCTGGAAATG 
GAGCCCACTT CAGAACGCGA ACCACTTGGA GGTCTCAAGC TGCTGTGTGC ACTCGCTGAG 
CAACGCATCC AGGAGGAGGT GGTGCAGGGC AGCAGTCTGT TTGCTACGCC GTCGTCGCGA 
ACGCCCACTC CCACGTCGCT GGCCCTGGGA ACGACAGCTG CGACGCCGCC CATTTTCGAG 
GCCAAGTCCT GTTATGCCTT TGGGCAATCG CAGGGTTCCG TCTTCCCCTC CTCAACATCC 
TCCTTTCAGA TGCCATTAAG CTCGCCCGGA TTCCCCTCGA TGCAGGGCAT CGAGCTGCCA 
ACCACGTCGG CTGGTATCGT TGCCGAACTG ACTCCTGTGA AGCGCAAGAA GCACAAACAT 
TCCAAGTCCT CAGGCAGCGA CAGCCGCAAG TCGGCACGTT GCAGCAAGAA AAGCAAGAAG 
AAGCGCCACC ACAGCAGCAG CCGCCAGCAG TTCTCCGCTC CGGCCGAGGA GGATCTGGAC 
CTGCAGGACG ATCAGCTGCA GTCGGAGCTA CGCAATGCCC TCCACGCCGT GGATCCCAGC 
TATGCGCAGC GCTTTGGCCA GGAGGTGTTT AGCATCATGG ACAATAGTAT GCGTATGCGA 
CTGGCGAAAA TAACGCGGCA GTATCGCAAG AAGAAACGGA AGCTGGATGA GATTTCCAAG 
CACAAAAAGA AGAAGAAGTG CTCCAAGCAG CAGCTCGAAC TGCAACAGCA GCAGGCATCG 
CAACAGGCGG TGCAGCCATC TTCAGCTCTG CAACAGCCAC AGATGACGCT GTCCAGTGTC 
CTGGGAACTT CATCCCCGTT GCGCGATTAC AAGTTCCCAA AGTTTTCGAG CAGTAACCTT 
CAGGCGTCTA GTTTTTTGCG ATTCCCGGAC AAGACGCACT CCTTCCCCCC ACCGCCGTCC 
CTACAGCAAT CCCAACCGGA GCCCAATCCC TTGCCAAGCA GCAGCTCACC GAGTACCTCG 
TCGTTTGTCC GCCTGGAACC CAGCGCATTG GACGCGGCCG TTGCCATTGC ACCAACTACC 
TCTTCGACTT CCACCTCCGG CTCACCATCC ACCAAGCAGG TGGCTTCCGC GCGGAAGCAG 
CGCAAGATGA AGGCGAGCAC CGTCGGCGCC GCTGGAGAGC AGACAGCGGC CACGGAGGCA 
AAGCGGCGAT TTAGCGCCAT TGATCGCGAG CTTCAGCTGA CCAGCGAGCA CCTGTATCGC 
GATGAGACAC GTGTCCTCAC GGACATGGGT GGTCTTTTCT ATGCGGGTGT AATGAAGCCA 
TTGCGGCCGC CAGATGTGTA TTCAATCACA TTGGATGGAG AGCGTGGCAA CAAGTCGCAT 
GTAATGTCGC GCGAGGATAT ACTCAAGGAT ACGATCCTCG AGGTAGCTCC GAAGAGCGTG 
GAGAGTGTAC CAGTAGGCAC GCGCCTGTGC GCCTACTGGA GTCAGCAGTA CCGATGCCTT 
TATCCAGGAC GTGCCATCGA CTCCGAGCAG GTCGTCGATG GAACGGCCAG CAGCGCTACG 
AATGCGGCTA CAACAGCGCC GGACTTCGTA AGCGTAGAGT TCGACGATGG CGACAGCGGC 
AGGATCCGCT TGCAGAACAT CCGTATGCTC CTCAGCGATT ATCCCATAGC AGTAGGCAAG 
CAGAAGCGAA GCGCTCTGCG CGGGGGTGAA AGTGGACCTG GCGGAGTCAC TCAGGACCAC 
TTGACCGTGC CCGGCTGCGA GGATTCGCAC AGCCATCATA GCCTGGGGAT GAGTAGCGAT 
AACACCACAT CGCTGGCGGC AACTATGGAG TTGTTCACGC AGCGCAGTGA GAAGAAGCGC 
CTGAAGAAGA GCCTCAAGAA GATGTCCAAA GCTCAAAATG GCGTTAGTCC GGCCACGGCA 
ACCAATGGTG GAGCTGATGC CGCTGCCTTG GGCGATGGTG TCAGTGCCGA AGACGCCGCT 
CGTAAGCACC ATAAGCACAA GAAACGCAAG AAGCACAAGA AGCATCACCG CAAGAATGGC 
AGCGAAGAGC AGGAGCAACA GGTAGTCCAG CAGGACTACT CTGCGGCAGG ACAGGAAGCG 
ACAGAGGCTC CTTCCACAGA GATGTCATCA GCGACCTCAA GTCGCGTTAA GGTGGAGGTC 
AAAGTGAAGA CAGAGCAGCT GGAGATGGAG GAGGAGGAGT CCGCATCCAA TCTCATGTCA 
GAGATATCCG ATGAGGCCAA GGGCGACGAT CTGGTCGAGC ATAACAACTC CAAGGGAAGC 
AGCAAGATTG CGGCCTTCCT GCCGGAACGG CAGCTATGGG GTTGGTATGG TACCGCCTAT 
CGCAAGGCGG GCGTCAAGGG ACGCGCCAGA AAGCAATTTT ACAAAACAAT CAAAAGGGGA 
AAGGAGACTA TCACGGTCGG GGACAGTGCA GTATTTCTAT CGACGGGCAG ACCAGATCGA 
CCCTATATCG GACGCATTGA GTCCATGTGG GAAACAACCA CGGGCAACAA GGTGGTGCGT 
GTGGCGTGGT TCTATCATCC GGAGGAGACG ACCGGCTGTC CAAAATTGAA ATTTCCGGGT 
GCTCTTTTTG AATCGCCGCA CGAGGATGAG AACGATGTGC AGACCATTTC GCATCGGTGT 
GAGGTGCTGC AGTTTGGCAG TTACTTCGAG AAGTTCGGTG CCGATTCGAA GCAATATCAA 
TCCATATACG ATAACAATGA TACATATTAT TTAGCCGGGC ACTATAACCC AAGGTTACAA 
GTGCTAAAAC TACAAGACGA TATTCCAACT CTCGAAGAGC TGCAGGATAC AAATACTACT 
ACTACTACTA CAGAAACTAC TACCGAGGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732791.2
CDS	628..5460
Translation	MATFNPGASSSAADVLSATTATATFLVPTTAAVSHPGAHTAHLQLDQFGGFSAATATPLQHHHHQQTNSSYTFVQIKREPCQVSEISSNNCHQQQQQHHQHHQHQVQHQGLSSASMTASSKTMSSSTLTTLVKIEAPSPKVSELEKSTGNSVPIGIAVARKRPQEALVPAINTPATLPLQPPLNKDLNCFGIRVADLGATSCGNLYFTGNGDLMTTGTATAEELALSAAGVNRAPSTFWQYPNPFQQAAAAAAFVWPSAYIPQAPAPGGHSAAAAAAAASLQPTPNLSSLPNFIFPSMGGHQALSTTPSYASTLQLYLAAAATATGSSTMCQHSNQQTSTTTTNSTINLSLAAGSGVTQSGNAASSLSSSSSLSASSSRFLSLATGQTGIPSLLSLPPPTGLKEEYPMPLALPPLVPLEAARDKEQALNLMRLPTPPTSATMEPSSLGHATPHHLFQSGAMATPTPALLNLSMHGNGGELPAATPLPAVSDEAALNYKLHAPLTPQTPPRLADIPVSGSTQLLPQMQDVNIQTDTPVCSEDESFPGSAKPQDPAAEAFPPPTHVQPLELTKPSEASHTQPTECHTQTEPSDIPSASQEESAEQTPPEPIETMTQATQADQTSPEDLTGLELLSNISTNSKPLVRVKQEPVEHIEQPAPQPQPVNIMPSEPTLPMPPMLEMEPTSEREPLGGLKLLCALAEQRIQEEVVQGSSLFATPSSRTPTPTSLALGTTAATPPIFEAKSCYAFGQSQGSVFPSSTSSFQMPLSSPGFPSMQGIELPTTSAGIVAELTPVKRKKHKHSKSSGSDSRKSARCSKKSKKKRHHSSSRQQFSAPAEEDLDLQDDQLQSELRNALHAVDPSYAQRFGQEVFSIMDNSMRMRLAKITRQYRKKKRKLDEISKHKKKKKCSKQQLELQQQQASQQAVQPSSALQQPQMTLSSVLGTSSPLRDYKFPKFSSSNLQASSFLRFPDKTHSFPPPPSLQQSQPEPNPLPSSSSPSTSSFVRLEPSALDAAVAIAPTTSSTSTSGSPSTKQVASARKQRKMKASTVGAAGEQTAATEAKRRFSAIDRELQLTSEHLYRDETRVLTDMGGLFYAGVMKPLRPPDVYSITLDGERGNKSHVMSREDILKDTILEVAPKSVESVPVGTRLCAYWSQQYRCLYPGRAIDSEQVVDGTASSATNAATTAPDFVSVEFDDGDSGRIRLQNIRMLLSDYPIAVGKQKRSALRGGESGPGGVTQDHLTVPGCEDSHSHHSLGMSSDNTTSLAATMELFTQRSEKKRLKKSLKKMSKAQNGVSPATATNGGADAAALGDGVSAEDAARKHHKHKKRKKHKKHHRKNGSEEQEQQVVQQDYSAAGQEATEAPSTEMSSATSSRVKVEVKVKTEQLEMEEEESASNLMSEISDEAKGDDLVEHNNSKGSSKIAAFLPERQLWGWYGTAYRKAGVKGRARKQFYKTIKRGKETITVGDSAVFLSTGRPDRPYIGRIESMWETTTGNKVVRVAWFYHPEETTGCPKLKFPGALFESPHEDENDVQTISHRCEVLQFGSYFEKFGADSKQYQSIYDNNDTYYLAGHYNPRLQVLKLQDDIPTLEELQDTNTTTTTTETTTED

Target ORF information:

RefSeq Version	NM_170038.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster winged eye (wge), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170038.2

ORF Insert Sequence:

ATGGCCACGT TTAATCCTGG CGCCAGCAGC AGTGCCGCCG ACGTTCTGAG CGCCACCACA 
GCAACGGCTA CTTTTCTGGT GCCCACCACT GCGGCAGTAT CGCATCCTGG TGCCCATACC 
GCCCATCTGC AGCTGGATCA ATTCGGAGGC TTCTCCGCGG CGACGGCCAC TCCCCTGCAG 
CACCATCACC ATCAGCAGAC CAACTCGTCG TACACTTTTG TGCAGATCAA GAGGGAACCC 
TGCCAGGTGT CCGAAATAAG CTCGAACAAT TGCCACCAGC AACAGCAGCA ACATCACCAA 
CACCACCAGC ACCAGGTGCA GCATCAGGGC CTGTCTTCCG CCTCGATGAC CGCCTCCTCC 
AAGACCATGT CCTCCTCCAC ACTGACCACT CTCGTCAAAA TAGAGGCTCC CTCGCCAAAG 
GTATCGGAAT TGGAAAAGTC CACAGGCAAC TCCGTTCCCA TTGGCATCGC CGTGGCCAGG 
AAACGCCCGC AAGAAGCTCT GGTGCCGGCT ATCAACACCC CCGCTACGCT GCCGCTGCAG 
CCACCACTCA ACAAGGACTT GAACTGCTTC GGCATACGCG TCGCTGACCT CGGTGCCACC 
AGTTGCGGCA ATTTGTACTT TACTGGCAAC GGCGACCTGA TGACCACCGG CACAGCCACC 
GCCGAGGAAC TGGCTCTGAG TGCAGCGGGT GTGAACCGGG CACCCTCCAC TTTCTGGCAG 
TATCCAAATC CGTTCCAGCA GGCAGCGGCT GCGGCGGCCT TCGTCTGGCC CTCGGCCTAC 
ATCCCCCAGG CGCCGGCACC TGGTGGTCAC TCTGCTGCGG CAGCCGCCGC AGCTGCTTCG 
CTGCAGCCGA CGCCCAACTT GTCCAGTTTG CCCAACTTCA TCTTTCCCTC GATGGGCGGC 
CACCAGGCGC TGAGCACAAC GCCTTCGTAC GCGTCCACCC TGCAGCTGTA TCTGGCCGCT 
GCGGCCACTG CGACGGGCAG CTCGACGATG TGCCAGCACA GCAATCAGCA GACCAGCACC 
ACAACCACCA ATTCCACCAT TAATCTGAGT CTGGCCGCTG GTTCGGGGGT CACGCAAAGC 
GGCAATGCGG CTAGCAGCTT AAGTTCCAGC AGTAGTCTCA GCGCGAGTAG CTCCCGTTTC 
CTCAGCCTGG CCACTGGTCA GACTGGGATT CCTTCACTTC TCTCCTTGCC GCCACCGACA 
GGTTTGAAAG AGGAGTACCC GATGCCGCTG GCATTGCCGC CCCTTGTGCC GCTCGAGGCG 
GCGCGGGATA AGGAGCAGGC CTTGAACCTA ATGCGTCTGC CCACGCCGCC CACCTCGGCC 
ACCATGGAGC CGTCTTCCCT AGGCCATGCA ACGCCCCACC ATCTCTTCCA GTCGGGTGCC 
ATGGCTACGC CCACGCCAGC ATTGCTCAAC CTATCGATGC ATGGCAACGG TGGGGAGCTG 
CCTGCTGCAA CGCCTCTGCC AGCGGTCAGT GATGAAGCGG CGCTGAACTA CAAGCTGCAT 
GCACCGTTGA CACCGCAGAC ACCTCCTCGC CTGGCGGACA TTCCTGTGTC GGGATCGACT 
CAGCTCTTGC CGCAAATGCA GGATGTCAAT ATACAGACGG ATACGCCCGT TTGCAGTGAA 
GACGAGAGCT TTCCGGGCTC AGCAAAGCCG CAGGATCCTG CTGCAGAAGC ATTTCCGCCC 
CCAACCCATG TCCAGCCGCT GGAACTAACG AAGCCCAGTG AAGCCAGCCA TACCCAGCCT 
ACTGAGTGCC ATACCCAGAC CGAACCCAGT GACATACCCA GTGCCAGCCA GGAGGAGTCA 
GCGGAGCAGA CTCCCCCGGA ACCCATAGAA ACGATGACGC AAGCCACGCA GGCAGATCAA 
ACGTCCCCCG AGGACCTGAC CGGTTTGGAG CTGCTCTCGA ACATAAGCAC CAATAGCAAA 
CCACTGGTGC GAGTAAAACA AGAGCCGGTG GAGCACATAG AGCAGCCAGC GCCGCAGCCG 
CAGCCTGTGA ATATAATGCC CTCAGAGCCT ACGCTGCCTA TGCCGCCAAT GCTGGAAATG 
GAGCCCACTT CAGAACGCGA ACCACTTGGA GGTCTCAAGC TGCTGTGTGC ACTCGCTGAG 
CAACGCATCC AGGAGGAGGT GGTGCAGGGC AGCAGTCTGT TTGCTACGCC GTCGTCGCGA 
ACGCCCACTC CCACGTCGCT GGCCCTGGGA ACGACAGCTG CGACGCCGCC CATTTTCGAG 
GCCAAGTCCT GTTATGCCTT TGGGCAATCG CAGGGTTCCG TCTTCCCCTC CTCAACATCC 
TCCTTTCAGA TGCCATTAAG CTCGCCCGGA TTCCCCTCGA TGCAGGGCAT CGAGCTGCCA 
ACCACGTCGG CTGGTATCGT TGCCGAACTG ACTCCTGTGA AGCGCAAGAA GCACAAACAT 
TCCAAGTCCT CAGGCAGCGA CAGCCGCAAG TCGGCACGTT GCAGCAAGAA AAGCAAGAAG 
AAGCGCCACC ACAGCAGCAG CCGCCAGCAG TTCTCCGCTC CGGCCGAGGA GGATCTGGAC 
CTGCAGGACG ATCAGCTGCA GTCGGAGCTA CGCAATGCCC TCCACGCCGT GGATCCCAGC 
TATGCGCAGC GCTTTGGCCA GGAGGTGTTT AGCATCATGG ACAATAGTAT GCGTATGCGA 
CTGGCGAAAA TAACGCGGCA GTATCGCAAG AAGAAACGGA AGCTGGATGA GATTTCCAAG 
CACAAAAAGA AGAAGAAGTG CTCCAAGCAG CAGCTCGAAC TGCAACAGCA GCAGGCATCG 
CAACAGGCGG TGCAGCCATC TTCAGCTCTG CAACAGCCAC AGATGACGCT GTCCAGTGTC 
CTGGGAACTT CATCCCCGTT GCGCGATTAC AAGTTCCCAA AGTTTTCGAG CAGTAACCTT 
CAGGCGTCTA GTTTTTTGCG ATTCCCGGAC AAGACGCACT CCTTCCCCCC ACCGCCGTCC 
CTACAGCAAT CCCAACCGGA GCCCAATCCC TTGCCAAGCA GCAGCTCACC GAGTACCTCG 
TCGTTTGTCC GCCTGGAACC CAGCGCATTG GACGCGGCCG TTGCCATTGC ACCAACTACC 
TCTTCGACTT CCACCTCCGG CTCACCATCC ACCAAGCAGG TGGCTTCCGC GCGGAAGCAG 
CGCAAGATGA AGGCGAGCAC CGTCGGCGCC GCTGGAGAGC AGACAGCGGC CACGGAGGCA 
AAGCGGCGAT TTAGCGCCAT TGATCGCGAG CTTCAGCTGA CCAGCGAGCA CCTGTATCGC 
GATGAGACAC GTGTCCTCAC GGACATGGGT GGTCTTTTCT ATGCGGGTGT AATGAAGCCA 
TTGCGGCCGC CAGATGTGTA TTCAATCACA TTGGATGGAG AGCGTGGCAA CAAGTCGCAT 
GTAATGTCGC GCGAGGATAT ACTCAAGGAT ACGATCCTCG AGGTAGCTCC GAAGAGCGTG 
GAGAGTGTAC CAGTAGGCAC GCGCCTGTGC GCCTACTGGA GTCAGCAGTA CCGATGCCTT 
TATCCAGGAC GTGCCATCGA CTCCGAGCAG GTCGTCGATG GAACGGCCAG CAGCGCTACG 
AATGCGGCTA CAACAGCGCC GGACTTCGTA AGCGTAGAGT TCGACGATGG CGACAGCGGC 
AGGATCCGCT TGCAGAACAT CCGTATGCTC CTCAGCGATT ATCCCATAGC AGTAGGCAAG 
CAGAAGCGAA GCGCTCTGCG CGGGGGTGAA AGTGGACCTG GCGGAGTCAC TCAGGACCAC 
TTGACCGTGC CCGGCTGCGA GGATTCGCAC AGCCATCATA GCCTGGGGAT GAGTAGCGAT 
AACACCACAT CGCTGGCGGC AACTATGGAG TTGTTCACGC AGCGCAGTGA GAAGAAGCGC 
CTGAAGAAGA GCCTCAAGAA GATGTCCAAA GCTCAAAATG GCGTTAGTCC GGCCACGGCA 
ACCAATGGTG GAGCTGATGC CGCTGCCTTG GGCGATGGTG TCAGTGCCGA AGACGCCGCT 
CGTAAGCACC ATAAGCACAA GAAACGCAAG AAGCACAAGA AGCATCACCG CAAGAATGGC 
AGCGAAGAGC AGGAGCAACA GGTAGTCCAG CAGGACTACT CTGCGGCAGG ACAGGAAGCG 
ACAGAGGCTC CTTCCACAGA GATGTCATCA GCGACCTCAA GTCGCGTTAA GGTGGAGGTC 
AAAGTGAAGA CAGAGCAGCT GGAGATGGAG GAGGAGGAGT CCGCATCCAA TCTCATGTCA 
GAGATATCCG ATGAGGCCAA GGGCGACGAT CTGGTCGAGC ATAACAACTC CAAGGGAAGC 
AGCAAGATTG CGGCCTTCCT GCCGGAACGG CAGCTATGGG GTTGGTATGG TACCGCCTAT 
CGCAAGGCGG GCGTCAAGGG ACGCGCCAGA AAGCAATTTT ACAAAACAAT CAAAAGGGGA 
AAGGAGACTA TCACGGTCGG GGACAGTGCA GTATTTCTAT CGACGGGCAG ACCAGATCGA 
CCCTATATCG GACGCATTGA GTCCATGTGG GAAACAACCA CGGGCAACAA GGTGGTGCGT 
GTGGCGTGGT TCTATCATCC GGAGGAGACG ACCGGCTGTC CAAAATTGAA ATTTCCGGGT 
GCTCTTTTTG AATCGCCGCA CGAGGATGAG AACGATGTGC AGACCATTTC GCATCGGTGT 
GAGGTGCTGC AGTTTGGCAG TTACTTCGAG AAGTTCGGTG CCGATTCGAA GCAATATCAA 
TCCATATACG ATAACAATGA TACATATTAT TTAGCCGGGC ACTATAACCC AAGGTTACAA 
GTGCTAAAAC TACAAGACGA TATTCCAACT CTCGAAGAGC TGCAGGATAC AAATACTACT 
ACTACTACTA CAGAAACTAC TACCGAGGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wge ( NM_001043281.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036746.1 Drosophila melanogaster winged eye (wge), transcript variant C, mRNA.

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CloneID	OFa08684
Clone ID Related Accession (Same CDS sequence)	NM_001043281.2
Accession Version	NM_001043281.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	winged eye, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001043281.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACGT TTAATCCTGG CGCCAGCAGC AGTGCCGCCG ACGTTCTGAG CGCCACCACA 
GCAACGGCTA CTTTTCTGGT GCCCACCACT GCGGCAGTAT CGCATCCTGG TGCCCATACC 
GCCCATCTGC AGCTGGATCA ATTCGGAGGC TTCTCCGCGG CGACGGCCAC TCCCCTGCAG 
CACCATCACC ATCAGCAGAC CAACTCGTCG TACACTTTTG TGCAGATCAA GAGGGAACCC 
TGCCAGGTGT CCGAAATAAG CTCGAACAAT TGCCACCAGC AACAGCAGCA ACATCACCAA 
CACCACCAGC ACCAGGTGCA GCATCAGGGC CTGTCTTCCG CCTCGATGAC CGCCTCCTCC 
AAGACCATGT CCTCCTCCAC ACTGACCACT CTCGTCAAAA TAGAGGCTCC CTCGCCAAAG 
GTATCGGAAT TGGAAAAGTC CACAGGCAAC TCCGTTCCCA TTGGCATCGC CGTGGCCAGG 
AAACGCCCGC AAGAAGCTCT GGTGCCGGCT ATCAACACCC CCGCTACGCT GCCGCTGCAG 
CCACCACTCA ACAAGGACTT GAACTGCTTC GGCATACGCG TCGCTGACCT CGGTGCCACC 
AGTTGCGGCA ATTTGTACTT TACTGGCAAC GGCGACCTGA TGACCACCGG CACAGCCACC 
GCCGAGGAAC TGGCTCTGAG TGCAGCGGGT GTGAACCGGG CACCCTCCAC TTTCTGGCAG 
TATCCAAATG CATTACCCAT TGAATCAGTG ATTTCCATGT CGCCCGCCAC GGTGGGCCTG 
CAGTACTCGC GGGAGGCCAG TCGCGGACAG GTGGTGCTGC TGCCGGCCGG CCCAACAGCG 
CTCGATCCGT TCCAGCAGGC AGCGGCTGCG GCGGCCTTCG TCTGGCCCTC GGCCTACATC 
CCCCAGGCGC CGGCACCTGG TGGTCACTCT GCTGCGGCAG CCGCCGCAGC TGCTTCGCTG 
CAGCCGACGC CCAACTTGTC CAGTTTGCCC AACTTCATCT TTCCCTCGAT GGGCGGCCAC 
CAGGCGCTGA GCACAACGCC TTCGTACGCG TCCACCCTGC AGCTGTATCT GGCCGCTGCG 
GCCACTGCGA CGGGCAGCTC GACGATGTGC CAGCACAGCA ATCAGCAGAC CAGCACCACA 
ACCACCAATT CCACCATTAA TCTGAGTCTG GCCGCTGGTT CGGGGGTCAC GCAAAGCGGC 
AATGCGGCTA GCAGCTTAAG TTCCAGCAGT AGTCTCAGCG CGAGTAGCTC CCGTTTCCTC 
AGCCTGGCCA CTGGTCAGAC TGGGATTCCT TCACTTCTCT CCTTGCCGCC ACCGACAGGT 
TTGAAAGAGG AGTACCCGAT GCCGCTGGCA TTGCCGCCCC TTGTGCCGCT CGAGGCGGCG 
CGGGATAAGG AGCAGGCCTT GAACCTAATG CGTCTGCCCA CGCCGCCCAC CTCGGCCACC 
ATGGAGCCGT CTTCCCTAGG CCATGCAACG CCCCACCATC TCTTCCAGTC GGGTGCCATG 
GCTACGCCCA CGCCAGCATT GCTCAACCTA TCGATGCATG GCAACGGTGG GGAGCTGCCT 
GCTGCAACGC CTCTGCCAGC GGTCAGTGAT GAAGCGGCGC TGAACTACAA GCTGCATGCA 
CCGTTGACAC CGCAGACACC TCCTCGCCTG GCGGACATTC CTGTGTCGGG ATCGACTCAG 
CTCTTGCCGC AAATGCAGGA TGTCAATATA CAGACGGATA CGCCCGTTTG CAGTGAAGAC 
GAGAGCTTTC CGGGCTCAGC AAAGCCGCAG GATCCTGCTG CAGAAGCATT TCCGCCCCCA 
ACCCATGTCC AGCCGCTGGA ACTAACGAAG CCCAGTGAAG CCAGCCATAC CCAGCCTACT 
GAGTGCCATA CCCAGACCGA ACCCAGTGAC ATACCCAGTG CCAGCCAGGA GGAGTCAGCG 
GAGCAGACTC CCCCGGAACC CATAGAAACG ATGACGCAAG CCACGCAGGC AGATCAAACG 
TCCCCCGAGG ACCTGACCGG TTTGGAGCTG CTCTCGAACA TAAGCACCAA TAGCAAACCA 
CTGGTGCGAG TAAAACAAGA GCCGGTGGAG CACATAGAGC AGCCAGCGCC GCAGCCGCAG 
CCTGTGAATA TAATGCCCTC AGAGCCTACG CTGCCTATGC CGCCAATGCT GGAAATGGAG 
CCCACTTCAG AACGCGAACC ACTTGGAGGT CTCAAGCTGC TGTGTGCACT CGCTGAGCAA 
CGCATCCAGG AGGAGGTGGT GCAGGGCAGC AGTCTGTTTG CTACGCCGTC GTCGCGAACG 
CCCACTCCCA CGTCGCTGGC CCTGGGAACG ACAGCTGCGA CGCCGCCCAT TTTCGAGGCC 
AAGTCCTGTT ATGCCTTTGG GCAATCGCAG GGTTCCGTCT TCCCCTCCTC AACATCCTCC 
TTTCAGATGC CATTAAGCTC GCCCGGATTC CCCTCGATGC AGGGCATCGA GCTGCCAACC 
ACGTCGGCTG GTATCGTTGC CGAACTGACT CCTGTGAAGC GCAAGAAGCA CAAACATTCC 
AAGTCCTCAG GCAGCGACAG CCGCAAGTCG GCACGTTGCA GCAAGAAAAG CAAGAAGAAG 
CGCCACCACA GCAGCAGCCG CCAGCAGTTC TCCGCTCCGG CCGAGGAGGA TCTGGACCTG 
CAGGACGATC AGCTGCAGTC GGAGCTACGC AATGCCCTCC ACGCCGTGGA TCCCAGCTAT 
GCGCAGCGCT TTGGCCAGGA GGTGTTTAGC ATCATGGACA ATAGTATGCG TATGCGACTG 
GCGAAAATAA CGCGGCAGTA TCGCAAGAAG AAACGGAAGC TGGATGAGAT TTCCAAGCAC 
AAAAAGAAGA AGAAGTGCTC CAAGCAGCAG CTCGAACTGC AACAGCAGCA GGCATCGCAA 
CAGGCGGTGC AGCCATCTTC AGCTCTGCAA CAGCCACAGA TGACGCTGTC CAGTGTCCTG 
GGAACTTCAT CCCCGTTGCG CGATTACAAG TTCCCAAAGT TTTCGAGCAG TAACCTTCAG 
GCGTCTAGTT TTTTGCGATT CCCGGACAAG ACGCACTCCT TCCCCCCACC GCCGTCCCTA 
CAGCAATCCC AACCGGAGCC CAATCCCTTG CCAAGCAGCA GCTCACCGAG TACCTCGTCG 
TTTGTCCGCC TGGAACCCAG CGCATTGGAC GCGGCCGTTG CCATTGCACC AACTACCTCT 
TCGACTTCCA CCTCCGGCTC ACCATCCACC AAGCAGGTGG CTTCCGCGCG GAAGCAGCGC 
AAGATGAAGG CGAGCACCGT CGGCGCCGCT GGAGAGCAGA CAGCGGCCAC GGAGGCAAAG 
CGGCGATTTA GCGCCATTGA TCGCGAGCTT CAGCTGACCA GCGAGCACCT GTATCGCGAT 
GAGACACGTG TCCTCACGGA CATGGGTGGT CTTTTCTATG CGGGTGTAAT GAAGCCATTG 
CGGCCGCCAG ATGTGTATTC AATCACATTG GATGGAGAGC GTGGCAACAA GTCGCATGTA 
ATGTCGCGCG AGGATATACT CAAGGATACG ATCCTCGAGG TAGCTCCGAA GAGCGTGGAG 
AGTGTACCAG TAGGCACGCG CCTGTGCGCC TACTGGAGTC AGCAGTACCG ATGCCTTTAT 
CCAGGACGTG CCATCGACTC CGAGCAGGTC GTCGATGGAA CGGCCAGCAG CGCTACGAAT 
GCGGCTACAA CAGCGCCGGA CTTCGTAAGC GTAGAGTTCG ACGATGGCGA CAGCGGCAGG 
ATCCGCTTGC AGAACATCCG TATGCTCCTC AGCGATTATC CCATAGCAGA GTACAACGAT 
AATCCCCTCT ACTCAGTAGG CAAGCAGAAG CGAAGCGCTC TGCGCGGGGG TGAAAGTGGA 
CCTGGCGGAG TCACTCAGGA CCACTTGACC GTGCCCGGCT GCGAGGATTC GCACAGCCAT 
CATAGCCTGG GGATGAGTAG CGATAACACC ACATCGCTGG CGGCAACTAT GGAGTTGTTC 
ACGCAGCGCA GTGAGAAGAA GCGCCTGAAG AAGAGCCTCA AGAAGATGTC CAAAGCTCAA 
AATGGCGTTA GTCCGGCCAC GGCAACCAAT GGTGGAGCTG ATGCCGCTGC CTTGGGCGAT 
GGTGTCAGTG CCGAAGACGC CGCTCGTAAG CACCATAAGC ACAAGAAACG CAAGAAGCAC 
AAGAAGCATC ACCGCAAGAA TGGCAGCGAA GAGCAGGAGC AACAGGTAGT CCAGCAGGAC 
TACTCTGCGG CAGGACAGGA AGCGACAGAG GCTCCTTCCA CAGAGATGTC ATCAGCGACC 
TCAAGTCGCG TTAAGGTGGA GGTCAAAGTG AAGACAGAGC AGCTGGAGAT GGAGGAGGAG 
GAGTCCGCAT CCAATCTCAT GTCAGAGATA TCCGATGAGG CCAAGGGCGA CGATCTGGTC 
GAGCATAACA ACTCCAAGGG AAGCAGCAAG ATTGCGGCCT TCCTGCCGGA ACGGCAGCTA 
TGGGGTTGGT ATGGTACCGC CTATCGCAAG GCGGGCGTCA AGGGACGCGC CAGAAAGCAA 
TTTTACAAAA CAATCAAAAG GGGAAAGGAG ACTATCACGG TCGGGGACAG TGCAGTATTT 
CTATCGACGG GCAGACCAGA TCGACCCTAT ATCGGACGCA TTGAGTCCAT GTGGGAAACA 
ACCACGGGCA ACAAGGTGGT GCGTGTGGCG TGGTTCTATC ATCCGGAGGA GACGACCGGC 
TGTCCAAAAT TGAAATTTCC GGGTGCTCTT TTTGAATCGC CGCACGAGGA TGAGAACGAT 
GTGCAGACCA TTTCGCATCG GTGTGAGGTG CTGCAGTTTG GCAGTTACTT CGAGAAGTTC 
GGTGCCGATT CGAAGCAATA TCAATCCATA TACGATAACA ATGATACATA TTATTTAGCC 
GGGCACTATA ACCCAAGGTT ACAAGTGCTA AAACTACAAG ACGATATTCC AACTCTCGAA 
GAGCTGCAGG ATACAAATAC TACTACTACT ACTACAGAAA CTACTACCGA GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036746.1
CDS	628..5604
Translation	MATFNPGASSSAADVLSATTATATFLVPTTAAVSHPGAHTAHLQLDQFGGFSAATATPLQHHHHQQTNSSYTFVQIKREPCQVSEISSNNCHQQQQQHHQHHQHQVQHQGLSSASMTASSKTMSSSTLTTLVKIEAPSPKVSELEKSTGNSVPIGIAVARKRPQEALVPAINTPATLPLQPPLNKDLNCFGIRVADLGATSCGNLYFTGNGDLMTTGTATAEELALSAAGVNRAPSTFWQYPNALPIESVISMSPATVGLQYSREASRGQVVLLPAGPTALDPFQQAAAAAAFVWPSAYIPQAPAPGGHSAAAAAAAASLQPTPNLSSLPNFIFPSMGGHQALSTTPSYASTLQLYLAAAATATGSSTMCQHSNQQTSTTTTNSTINLSLAAGSGVTQSGNAASSLSSSSSLSASSSRFLSLATGQTGIPSLLSLPPPTGLKEEYPMPLALPPLVPLEAARDKEQALNLMRLPTPPTSATMEPSSLGHATPHHLFQSGAMATPTPALLNLSMHGNGGELPAATPLPAVSDEAALNYKLHAPLTPQTPPRLADIPVSGSTQLLPQMQDVNIQTDTPVCSEDESFPGSAKPQDPAAEAFPPPTHVQPLELTKPSEASHTQPTECHTQTEPSDIPSASQEESAEQTPPEPIETMTQATQADQTSPEDLTGLELLSNISTNSKPLVRVKQEPVEHIEQPAPQPQPVNIMPSEPTLPMPPMLEMEPTSEREPLGGLKLLCALAEQRIQEEVVQGSSLFATPSSRTPTPTSLALGTTAATPPIFEAKSCYAFGQSQGSVFPSSTSSFQMPLSSPGFPSMQGIELPTTSAGIVAELTPVKRKKHKHSKSSGSDSRKSARCSKKSKKKRHHSSSRQQFSAPAEEDLDLQDDQLQSELRNALHAVDPSYAQRFGQEVFSIMDNSMRMRLAKITRQYRKKKRKLDEISKHKKKKKCSKQQLELQQQQASQQAVQPSSALQQPQMTLSSVLGTSSPLRDYKFPKFSSSNLQASSFLRFPDKTHSFPPPPSLQQSQPEPNPLPSSSSPSTSSFVRLEPSALDAAVAIAPTTSSTSTSGSPSTKQVASARKQRKMKASTVGAAGEQTAATEAKRRFSAIDRELQLTSEHLYRDETRVLTDMGGLFYAGVMKPLRPPDVYSITLDGERGNKSHVMSREDILKDTILEVAPKSVESVPVGTRLCAYWSQQYRCLYPGRAIDSEQVVDGTASSATNAATTAPDFVSVEFDDGDSGRIRLQNIRMLLSDYPIAEYNDNPLYSVGKQKRSALRGGESGPGGVTQDHLTVPGCEDSHSHHSLGMSSDNTTSLAATMELFTQRSEKKRLKKSLKKMSKAQNGVSPATATNGGADAAALGDGVSAEDAARKHHKHKKRKKHKKHHRKNGSEEQEQQVVQQDYSAAGQEATEAPSTEMSSATSSRVKVEVKVKTEQLEMEEEESASNLMSEISDEAKGDDLVEHNNSKGSSKIAAFLPERQLWGWYGTAYRKAGVKGRARKQFYKTIKRGKETITVGDSAVFLSTGRPDRPYIGRIESMWETTTGNKVVRVAWFYHPEETTGCPKLKFPGALFESPHEDENDVQTISHRCEVLQFGSYFEKFGADSKQYQSIYDNNDTYYLAGHYNPRLQVLKLQDDIPTLEELQDTNTTTTTTETTTED

Target ORF information:

RefSeq Version	NM_001043281.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster winged eye (wge), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043281.2

ORF Insert Sequence:

ATGGCCACGT TTAATCCTGG CGCCAGCAGC AGTGCCGCCG ACGTTCTGAG CGCCACCACA 
GCAACGGCTA CTTTTCTGGT GCCCACCACT GCGGCAGTAT CGCATCCTGG TGCCCATACC 
GCCCATCTGC AGCTGGATCA ATTCGGAGGC TTCTCCGCGG CGACGGCCAC TCCCCTGCAG 
CACCATCACC ATCAGCAGAC CAACTCGTCG TACACTTTTG TGCAGATCAA GAGGGAACCC 
TGCCAGGTGT CCGAAATAAG CTCGAACAAT TGCCACCAGC AACAGCAGCA ACATCACCAA 
CACCACCAGC ACCAGGTGCA GCATCAGGGC CTGTCTTCCG CCTCGATGAC CGCCTCCTCC 
AAGACCATGT CCTCCTCCAC ACTGACCACT CTCGTCAAAA TAGAGGCTCC CTCGCCAAAG 
GTATCGGAAT TGGAAAAGTC CACAGGCAAC TCCGTTCCCA TTGGCATCGC CGTGGCCAGG 
AAACGCCCGC AAGAAGCTCT GGTGCCGGCT ATCAACACCC CCGCTACGCT GCCGCTGCAG 
CCACCACTCA ACAAGGACTT GAACTGCTTC GGCATACGCG TCGCTGACCT CGGTGCCACC 
AGTTGCGGCA ATTTGTACTT TACTGGCAAC GGCGACCTGA TGACCACCGG CACAGCCACC 
GCCGAGGAAC TGGCTCTGAG TGCAGCGGGT GTGAACCGGG CACCCTCCAC TTTCTGGCAG 
TATCCAAATG CATTACCCAT TGAATCAGTG ATTTCCATGT CGCCCGCCAC GGTGGGCCTG 
CAGTACTCGC GGGAGGCCAG TCGCGGACAG GTGGTGCTGC TGCCGGCCGG CCCAACAGCG 
CTCGATCCGT TCCAGCAGGC AGCGGCTGCG GCGGCCTTCG TCTGGCCCTC GGCCTACATC 
CCCCAGGCGC CGGCACCTGG TGGTCACTCT GCTGCGGCAG CCGCCGCAGC TGCTTCGCTG 
CAGCCGACGC CCAACTTGTC CAGTTTGCCC AACTTCATCT TTCCCTCGAT GGGCGGCCAC 
CAGGCGCTGA GCACAACGCC TTCGTACGCG TCCACCCTGC AGCTGTATCT GGCCGCTGCG 
GCCACTGCGA CGGGCAGCTC GACGATGTGC CAGCACAGCA ATCAGCAGAC CAGCACCACA 
ACCACCAATT CCACCATTAA TCTGAGTCTG GCCGCTGGTT CGGGGGTCAC GCAAAGCGGC 
AATGCGGCTA GCAGCTTAAG TTCCAGCAGT AGTCTCAGCG CGAGTAGCTC CCGTTTCCTC 
AGCCTGGCCA CTGGTCAGAC TGGGATTCCT TCACTTCTCT CCTTGCCGCC ACCGACAGGT 
TTGAAAGAGG AGTACCCGAT GCCGCTGGCA TTGCCGCCCC TTGTGCCGCT CGAGGCGGCG 
CGGGATAAGG AGCAGGCCTT GAACCTAATG CGTCTGCCCA CGCCGCCCAC CTCGGCCACC 
ATGGAGCCGT CTTCCCTAGG CCATGCAACG CCCCACCATC TCTTCCAGTC GGGTGCCATG 
GCTACGCCCA CGCCAGCATT GCTCAACCTA TCGATGCATG GCAACGGTGG GGAGCTGCCT 
GCTGCAACGC CTCTGCCAGC GGTCAGTGAT GAAGCGGCGC TGAACTACAA GCTGCATGCA 
CCGTTGACAC CGCAGACACC TCCTCGCCTG GCGGACATTC CTGTGTCGGG ATCGACTCAG 
CTCTTGCCGC AAATGCAGGA TGTCAATATA CAGACGGATA CGCCCGTTTG CAGTGAAGAC 
GAGAGCTTTC CGGGCTCAGC AAAGCCGCAG GATCCTGCTG CAGAAGCATT TCCGCCCCCA 
ACCCATGTCC AGCCGCTGGA ACTAACGAAG CCCAGTGAAG CCAGCCATAC CCAGCCTACT 
GAGTGCCATA CCCAGACCGA ACCCAGTGAC ATACCCAGTG CCAGCCAGGA GGAGTCAGCG 
GAGCAGACTC CCCCGGAACC CATAGAAACG ATGACGCAAG CCACGCAGGC AGATCAAACG 
TCCCCCGAGG ACCTGACCGG TTTGGAGCTG CTCTCGAACA TAAGCACCAA TAGCAAACCA 
CTGGTGCGAG TAAAACAAGA GCCGGTGGAG CACATAGAGC AGCCAGCGCC GCAGCCGCAG 
CCTGTGAATA TAATGCCCTC AGAGCCTACG CTGCCTATGC CGCCAATGCT GGAAATGGAG 
CCCACTTCAG AACGCGAACC ACTTGGAGGT CTCAAGCTGC TGTGTGCACT CGCTGAGCAA 
CGCATCCAGG AGGAGGTGGT GCAGGGCAGC AGTCTGTTTG CTACGCCGTC GTCGCGAACG 
CCCACTCCCA CGTCGCTGGC CCTGGGAACG ACAGCTGCGA CGCCGCCCAT TTTCGAGGCC 
AAGTCCTGTT ATGCCTTTGG GCAATCGCAG GGTTCCGTCT TCCCCTCCTC AACATCCTCC 
TTTCAGATGC CATTAAGCTC GCCCGGATTC CCCTCGATGC AGGGCATCGA GCTGCCAACC 
ACGTCGGCTG GTATCGTTGC CGAACTGACT CCTGTGAAGC GCAAGAAGCA CAAACATTCC 
AAGTCCTCAG GCAGCGACAG CCGCAAGTCG GCACGTTGCA GCAAGAAAAG CAAGAAGAAG 
CGCCACCACA GCAGCAGCCG CCAGCAGTTC TCCGCTCCGG CCGAGGAGGA TCTGGACCTG 
CAGGACGATC AGCTGCAGTC GGAGCTACGC AATGCCCTCC ACGCCGTGGA TCCCAGCTAT 
GCGCAGCGCT TTGGCCAGGA GGTGTTTAGC ATCATGGACA ATAGTATGCG TATGCGACTG 
GCGAAAATAA CGCGGCAGTA TCGCAAGAAG AAACGGAAGC TGGATGAGAT TTCCAAGCAC 
AAAAAGAAGA AGAAGTGCTC CAAGCAGCAG CTCGAACTGC AACAGCAGCA GGCATCGCAA 
CAGGCGGTGC AGCCATCTTC AGCTCTGCAA CAGCCACAGA TGACGCTGTC CAGTGTCCTG 
GGAACTTCAT CCCCGTTGCG CGATTACAAG TTCCCAAAGT TTTCGAGCAG TAACCTTCAG 
GCGTCTAGTT TTTTGCGATT CCCGGACAAG ACGCACTCCT TCCCCCCACC GCCGTCCCTA 
CAGCAATCCC AACCGGAGCC CAATCCCTTG CCAAGCAGCA GCTCACCGAG TACCTCGTCG 
TTTGTCCGCC TGGAACCCAG CGCATTGGAC GCGGCCGTTG CCATTGCACC AACTACCTCT 
TCGACTTCCA CCTCCGGCTC ACCATCCACC AAGCAGGTGG CTTCCGCGCG GAAGCAGCGC 
AAGATGAAGG CGAGCACCGT CGGCGCCGCT GGAGAGCAGA CAGCGGCCAC GGAGGCAAAG 
CGGCGATTTA GCGCCATTGA TCGCGAGCTT CAGCTGACCA GCGAGCACCT GTATCGCGAT 
GAGACACGTG TCCTCACGGA CATGGGTGGT CTTTTCTATG CGGGTGTAAT GAAGCCATTG 
CGGCCGCCAG ATGTGTATTC AATCACATTG GATGGAGAGC GTGGCAACAA GTCGCATGTA 
ATGTCGCGCG AGGATATACT CAAGGATACG ATCCTCGAGG TAGCTCCGAA GAGCGTGGAG 
AGTGTACCAG TAGGCACGCG CCTGTGCGCC TACTGGAGTC AGCAGTACCG ATGCCTTTAT 
CCAGGACGTG CCATCGACTC CGAGCAGGTC GTCGATGGAA CGGCCAGCAG CGCTACGAAT 
GCGGCTACAA CAGCGCCGGA CTTCGTAAGC GTAGAGTTCG ACGATGGCGA CAGCGGCAGG 
ATCCGCTTGC AGAACATCCG TATGCTCCTC AGCGATTATC CCATAGCAGA GTACAACGAT 
AATCCCCTCT ACTCAGTAGG CAAGCAGAAG CGAAGCGCTC TGCGCGGGGG TGAAAGTGGA 
CCTGGCGGAG TCACTCAGGA CCACTTGACC GTGCCCGGCT GCGAGGATTC GCACAGCCAT 
CATAGCCTGG GGATGAGTAG CGATAACACC ACATCGCTGG CGGCAACTAT GGAGTTGTTC 
ACGCAGCGCA GTGAGAAGAA GCGCCTGAAG AAGAGCCTCA AGAAGATGTC CAAAGCTCAA 
AATGGCGTTA GTCCGGCCAC GGCAACCAAT GGTGGAGCTG ATGCCGCTGC CTTGGGCGAT 
GGTGTCAGTG CCGAAGACGC CGCTCGTAAG CACCATAAGC ACAAGAAACG CAAGAAGCAC 
AAGAAGCATC ACCGCAAGAA TGGCAGCGAA GAGCAGGAGC AACAGGTAGT CCAGCAGGAC 
TACTCTGCGG CAGGACAGGA AGCGACAGAG GCTCCTTCCA CAGAGATGTC ATCAGCGACC 
TCAAGTCGCG TTAAGGTGGA GGTCAAAGTG AAGACAGAGC AGCTGGAGAT GGAGGAGGAG 
GAGTCCGCAT CCAATCTCAT GTCAGAGATA TCCGATGAGG CCAAGGGCGA CGATCTGGTC 
GAGCATAACA ACTCCAAGGG AAGCAGCAAG ATTGCGGCCT TCCTGCCGGA ACGGCAGCTA 
TGGGGTTGGT ATGGTACCGC CTATCGCAAG GCGGGCGTCA AGGGACGCGC CAGAAAGCAA 
TTTTACAAAA CAATCAAAAG GGGAAAGGAG ACTATCACGG TCGGGGACAG TGCAGTATTT 
CTATCGACGG GCAGACCAGA TCGACCCTAT ATCGGACGCA TTGAGTCCAT GTGGGAAACA 
ACCACGGGCA ACAAGGTGGT GCGTGTGGCG TGGTTCTATC ATCCGGAGGA GACGACCGGC 
TGTCCAAAAT TGAAATTTCC GGGTGCTCTT TTTGAATCGC CGCACGAGGA TGAGAACGAT 
GTGCAGACCA TTTCGCATCG GTGTGAGGTG CTGCAGTTTG GCAGTTACTT CGAGAAGTTC 
GGTGCCGATT CGAAGCAATA TCAATCCATA TACGATAACA ATGATACATA TTATTTAGCC 
GGGCACTATA ACCCAAGGTT ACAAGTGCTA AAACTACAAG ACGATATTCC AACTCTCGAA 
GAGCTGCAGG ATACAAATAC TACTACTACT ACTACAGAAA CTACTACCGA GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wge ( NM_170037.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732790.3 Drosophila melanogaster winged eye (wge), transcript variant D, mRNA.

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CloneID	OFa08685
Clone ID Related Accession (Same CDS sequence)	NM_170037.2
Accession Version	NM_170037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	winged eye, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170037.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACGT TTAATCCTGG CGCCAGCAGC AGTGCCGCCG ACGTTCTGAG CGCCACCACA 
GCAACGGCTA CTTTTCTGGT GCCCACCACT GCGGCAGTAT CGCATCCTGG TGCCCATACC 
GCCCATCTGC AGCTGGATCA ATTCGGAGGC TTCTCCGCGG CGACGGCCAC TCCCCTGCAG 
CACCATCACC ATCAGCAGAC CAACTCGTCG TACACTTTTG TGCAGATCAA GAGGGAACCC 
TGCCAGGTGT CCGAAATAAG CTCGAACAAT TGCCACCAGC AACAGCAGCA ACATCACCAA 
CACCACCAGC ACCAGGTGCA GCATCAGGGC CTGTCTTCCG CCTCGATGAC CGCCTCCTCC 
AAGACCATGT CCTCCTCCAC ACTGACCACT CTCGTCAAAA TAGAGGCTCC CTCGCCAAAG 
GTATCGGAAT TGGAAAAGTC CACAGGCAAC TCCGTTCCCA TTGGCATCGC CGTGGCCAGG 
AAACGCCCGC AAGAAGCTCT GGTGCCGGCT ATCAACACCC CCGCTACGCT GCCGCTGCAG 
CCACCACTCA ACAAGGACTT GAACTGCTTC GGCATACGCG TCGCTGACCT CGGTGCCACC 
AGTTGCGGCA ATTTGTACTT TACTGGCAAC GGCGACCTGA TGACCACCGG CACAGCCACC 
GCCGAGGAAC TGGCTCTGAG TGCAGCGGGT GTGAACCGGG CACCCTCCAC TTTCTGGCAG 
TATCCAAATG CATTACCCAT TGAATCAGTG ATTTCCATGT CGCCCGCCAC GGTGGGCCTG 
CAGTACTCGC GGGAGGCCAG TCGCGGACAG GTGGTGCTGC TGCCGGCCGG CCCAACAGCG 
CTCGGCATAT CAAATTTTCC CCTCTTAACG CCCACAGATC CGTTCCAGCA GGCAGCGGCT 
GCGGCGGCCT TCGTCTGGCC CTCGGCCTAC ATCCCCCAGG CGCCGGCACC TGGTGGTCAC 
TCTGCTGCGG CAGCCGCCGC AGCTGCTTCG CTGCAGCCGA CGCCCAACTT GTCCAGTTTG 
CCCAACTTCA TCTTTCCCTC GATGGGCGGC CACCAGGCGC TGAGCACAAC GCCTTCGTAC 
GCGTCCACCC TGCAGCTGTA TCTGGCCGCT GCGGCCACTG CGACGGGCAG CTCGACGATG 
TGCCAGCACA GCAATCAGCA GACCAGCACC ACAACCACCA ATTCCACCAT TAATCTGAGT 
CTGGCCGCTG GTTCGGGGGT CACGCAAAGC GGCAATGCGG CTAGCAGCTT AAGTTCCAGC 
AGTAGTCTCA GCGCGAGTAG CTCCCGTTTC CTCAGCCTGG CCACTGGTCA GACTGGGATT 
CCTTCACTTC TCTCCTTGCC GCCACCGACA GGTTTGAAAG AGGAGTACCC GATGCCGCTG 
GCATTGCCGC CCCTTGTGCC GCTCGAGGCG GCGCGGGATA AGGAGCAGGC CTTGAACCTA 
ATGCGTCTGC CCACGCCGCC CACCTCGGCC ACCATGGAGC CGTCTTCCCT AGGCCATGCA 
ACGCCCCACC ATCTCTTCCA GTCGGGTGCC ATGGCTACGC CCACGCCAGC ATTGCTCAAC 
CTATCGATGC ATGGCAACGG TGGGGAGCTG CCTGCTGCAA CGCCTCTGCC AGCGGTCAGT 
GATGAAGCGG CGCTGAACTA CAAGCTGCAT GCACCGTTGA CACCGCAGAC ACCTCCTCGC 
CTGGCGGACA TTCCTGTGTC GGGATCGACT CAGCTCTTGC CGCAAATGCA GGATGTCAAT 
ATACAGACGG ATACGCCCGT TTGCAGTGAA GACGAGAGCT TTCCGGGCTC AGCAAAGCCG 
CAGGATCCTG CTGCAGAAGC ATTTCCGCCC CCAACCCATG TCCAGCCGCT GGAACTAACG 
AAGCCCAGTG AAGCCAGCCA TACCCAGCCT ACTGAGTGCC ATACCCAGAC CGAACCCAGT 
GACATACCCA GTGCCAGCCA GGAGGAGTCA GCGGAGCAGA CTCCCCCGGA ACCCATAGAA 
ACGATGACGC AAGCCACGCA GGCAGATCAA ACGTCCCCCG AGGACCTGAC CGGTTTGGAG 
CTGCTCTCGA ACATAAGCAC CAATAGCAAA CCACTGGTGC GAGTAAAACA AGAGCCGGTG 
GAGCACATAG AGCAGCCAGC GCCGCAGCCG CAGCCTGTGA ATATAATGCC CTCAGAGCCT 
ACGCTGCCTA TGCCGCCAAT GCTGGAAATG GAGCCCACTT CAGAACGCGA ACCACTTGGA 
GGTCTCAAGC TGCTGTGTGC ACTCGCTGAG CAACGCATCC AGGAGGAGGT GGTGCAGGGC 
AGCAGTCTGT TTGCTACGCC GTCGTCGCGA ACGCCCACTC CCACGTCGCT GGCCCTGGGA 
ACGACAGCTG CGACGCCGCC CATTTTCGAG GCCAAGTCCT GTTATGCCTT TGGGCAATCG 
CAGGGTTCCG TCTTCCCCTC CTCAACATCC TCCTTTCAGA TGCCATTAAG CTCGCCCGGA 
TTCCCCTCGA TGCAGGGCAT CGAGCTGCCA ACCACGTCGG CTGGTATCGT TGCCGAACTG 
ACTCCTGTGA AGCGCAAGAA GCACAAACAT TCCAAGTCCT CAGGCAGCGA CAGCCGCAAG 
TCGGCACGTT GCAGCAAGAA AAGCAAGAAG AAGCGCCACC ACAGCAGCAG CCGCCAGCAG 
TTCTCCGCTC CGGCCGAGGA GGATCTGGAC CTGCAGGACG ATCAGCTGCA GTCGGAGCTA 
CGCAATGCCC TCCACGCCGT GGATCCCAGC TATGCGCAGC GCTTTGGCCA GGAGGTGTTT 
AGCATCATGG ACAATAGTAT GCGTATGCGA CTGGCGAAAA TAACGCGGCA GTATCGCAAG 
AAGAAACGGA AGCTGGATGA GATTTCCAAG CACAAAAAGA AGAAGAAGTG CTCCAAGCAG 
CAGCTCGAAC TGCAACAGCA GCAGGCATCG CAACAGGCGG TGCAGCCATC TTCAGCTCTG 
CAACAGCCAC AGATGACGCT GTCCAGTGTC CTGGGAACTT CATCCCCGTT GCGCGATTAC 
AAGTTCCCAA AGTTTTCGAG CAGTAACCTT CAGGCGTCTA GTTTTTTGCG ATTCCCGGAC 
AAGACGCACT CCTTCCCCCC ACCGCCGTCC CTACAGCAAT CCCAACCGGA GCCCAATCCC 
TTGCCAAGCA GCAGCTCACC GAGTACCTCG TCGTTTGTCC GCCTGGAACC CAGCGCATTG 
GACGCGGCCG TTGCCATTGC ACCAACTACC TCTTCGACTT CCACCTCCGG CTCACCATCC 
ACCAAGCAGG TGGCTTCCGC GCGGAAGCAG CGCAAGATGA AGGCGAGCAC CGTCGGCGCC 
GCTGGAGAGC AGACAGCGGC CACGGAGGCA AAGCGGCGAT TTAGCGCCAT TGATCGCGAG 
CTTCAGCTGA CCAGCGAGCA CCTGTATCGC GATGAGACAC GTGTCCTCAC GGACATGGGT 
GGTCTTTTCT ATGCGGGTGT AATGAAGCCA TTGCGGCCGC CAGATGTGTA TTCAATCACA 
TTGGATGGAG AGCGTGGCAA CAAGTCGCAT GTAATGTCGC GCGAGGATAT ACTCAAGGAT 
ACGATCCTCG AGGTAGCTCC GAAGAGCGTG GAGAGTGTAC CAGTAGGCAC GCGCCTGTGC 
GCCTACTGGA GTCAGCAGTA CCGATGCCTT TATCCAGGAC GTGCCATCGA CTCCGAGCAG 
GTCGTCGATG GAACGGCCAG CAGCGCTACG AATGCGGCTA CAACAGCGCC GGACTTCGTA 
AGCGTAGAGT TCGACGATGG CGACAGCGGC AGGATCCGCT TGCAGAACAT CCGTATGCTC 
CTCAGCGATT ATCCCATAGC AGAGTACAAC GATAATCCCC TCTACTCAGT AGGCAAGCAG 
AAGCGAAGCG CTCTGCGCGG GGGTGAAAGT GGACCTGGCG GAGTCACTCA GGACCACTTG 
ACCGTGCCCG GCTGCGAGGA TTCGCACAGC CATCATAGCC TGGGGATGAG TAGCGATAAC 
ACCACATCGC TGGCGGCAAC TATGGAGTTG TTCACGCAGC GCAGTGAGAA GAAGCGCCTG 
AAGAAGAGCC TCAAGAAGAT GTCCAAAGCT CAAAATGGCG TTAGTCCGGC CACGGCAACC 
AATGGTGGAG CTGATGCCGC TGCCTTGGGC GATGGTGTCA GTGCCGAAGA CGCCGCTCGT 
AAGCACCATA AGCACAAGAA ACGCAAGAAG CACAAGAAGC ATCACCGCAA GAATGGCAGC 
GAAGAGCAGG AGCAACAGGT AGTCCAGCAG GACTACTCTG CGGCAGGACA GGAAGCGACA 
GAGGCTCCTT CCACAGAGAT GTCATCAGCG ACCTCAAGTC GCGTTAAGGT GGAGGTCAAA 
GTGAAGACAG AGCAGCTGGA GATGGAGGAG GAGGAGTCCG CATCCAATCT CATGTCAGAG 
ATATCCGATG AGGCCAAGGG CGACGATCTG GTCGAGCATA ACAACTCCAA GGGAAGCAGC 
AAGATTGCGG CCTTCCTGCC GGAACGGCAG CTATGGGGTT GGTATGGTAC CGCCTATCGC 
AAGGCGGGCG TCAAGGGACG CGCCAGAAAG CAATTTTACA AAACAATCAA AAGGGGAAAG 
GAGACTATCA CGGTCGGGGA CAGTGCAGTA TTTCTATCGA CGGGCAGACC AGATCGACCC 
TATATCGGAC GCATTGAGTC CATGTGGGAA ACAACCACGG GCAACAAGGT GGTGCGTGTG 
GCGTGGTTCT ATCATCCGGA GGAGACGACC GGCTGTCCAA AATTGAAATT TCCGGGTGCT 
CTTTTTGAAT CGCCGCACGA GGATGAGAAC GATGTGCAGA CCATTTCGCA TCGGTGTGAG 
GTGCTGCAGT TTGGCAGTTA CTTCGAGAAG TTCGGTGCCG ATTCGAAGCA ATATCAATCC 
ATATACGATA ACAATGATAC ATATTATTTA GCCGGGCACT ATAACCCAAG GTTACAAGTG 
CTAAAACTAC AAGACGATAT TCCAACTCTC GAAGAGCTGC AGGATACAAA TACTACTACT 
ACTACTACAG AAACTACTAC CGAGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732790.3
CDS	628..5637
Translation	MATFNPGASSSAADVLSATTATATFLVPTTAAVSHPGAHTAHLQLDQFGGFSAATATPLQHHHHQQTNSSYTFVQIKREPCQVSEISSNNCHQQQQQHHQHHQHQVQHQGLSSASMTASSKTMSSSTLTTLVKIEAPSPKVSELEKSTGNSVPIGIAVARKRPQEALVPAINTPATLPLQPPLNKDLNCFGIRVADLGATSCGNLYFTGNGDLMTTGTATAEELALSAAGVNRAPSTFWQYPNALPIESVISMSPATVGLQYSREASRGQVVLLPAGPTALGISNFPLLTPTDPFQQAAAAAAFVWPSAYIPQAPAPGGHSAAAAAAAASLQPTPNLSSLPNFIFPSMGGHQALSTTPSYASTLQLYLAAAATATGSSTMCQHSNQQTSTTTTNSTINLSLAAGSGVTQSGNAASSLSSSSSLSASSSRFLSLATGQTGIPSLLSLPPPTGLKEEYPMPLALPPLVPLEAARDKEQALNLMRLPTPPTSATMEPSSLGHATPHHLFQSGAMATPTPALLNLSMHGNGGELPAATPLPAVSDEAALNYKLHAPLTPQTPPRLADIPVSGSTQLLPQMQDVNIQTDTPVCSEDESFPGSAKPQDPAAEAFPPPTHVQPLELTKPSEASHTQPTECHTQTEPSDIPSASQEESAEQTPPEPIETMTQATQADQTSPEDLTGLELLSNISTNSKPLVRVKQEPVEHIEQPAPQPQPVNIMPSEPTLPMPPMLEMEPTSEREPLGGLKLLCALAEQRIQEEVVQGSSLFATPSSRTPTPTSLALGTTAATPPIFEAKSCYAFGQSQGSVFPSSTSSFQMPLSSPGFPSMQGIELPTTSAGIVAELTPVKRKKHKHSKSSGSDSRKSARCSKKSKKKRHHSSSRQQFSAPAEEDLDLQDDQLQSELRNALHAVDPSYAQRFGQEVFSIMDNSMRMRLAKITRQYRKKKRKLDEISKHKKKKKCSKQQLELQQQQASQQAVQPSSALQQPQMTLSSVLGTSSPLRDYKFPKFSSSNLQASSFLRFPDKTHSFPPPPSLQQSQPEPNPLPSSSSPSTSSFVRLEPSALDAAVAIAPTTSSTSTSGSPSTKQVASARKQRKMKASTVGAAGEQTAATEAKRRFSAIDRELQLTSEHLYRDETRVLTDMGGLFYAGVMKPLRPPDVYSITLDGERGNKSHVMSREDILKDTILEVAPKSVESVPVGTRLCAYWSQQYRCLYPGRAIDSEQVVDGTASSATNAATTAPDFVSVEFDDGDSGRIRLQNIRMLLSDYPIAEYNDNPLYSVGKQKRSALRGGESGPGGVTQDHLTVPGCEDSHSHHSLGMSSDNTTSLAATMELFTQRSEKKRLKKSLKKMSKAQNGVSPATATNGGADAAALGDGVSAEDAARKHHKHKKRKKHKKHHRKNGSEEQEQQVVQQDYSAAGQEATEAPSTEMSSATSSRVKVEVKVKTEQLEMEEEESASNLMSEISDEAKGDDLVEHNNSKGSSKIAAFLPERQLWGWYGTAYRKAGVKGRARKQFYKTIKRGKETITVGDSAVFLSTGRPDRPYIGRIESMWETTTGNKVVRVAWFYHPEETTGCPKLKFPGALFESPHEDENDVQTISHRCEVLQFGSYFEKFGADSKQYQSIYDNNDTYYLAGHYNPRLQVLKLQDDIPTLEELQDTNTTTTTTETTTED

Target ORF information:

RefSeq Version	NM_170037.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster winged eye (wge), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170037.2

ORF Insert Sequence:

ATGGCCACGT TTAATCCTGG CGCCAGCAGC AGTGCCGCCG ACGTTCTGAG CGCCACCACA 
GCAACGGCTA CTTTTCTGGT GCCCACCACT GCGGCAGTAT CGCATCCTGG TGCCCATACC 
GCCCATCTGC AGCTGGATCA ATTCGGAGGC TTCTCCGCGG CGACGGCCAC TCCCCTGCAG 
CACCATCACC ATCAGCAGAC CAACTCGTCG TACACTTTTG TGCAGATCAA GAGGGAACCC 
TGCCAGGTGT CCGAAATAAG CTCGAACAAT TGCCACCAGC AACAGCAGCA ACATCACCAA 
CACCACCAGC ACCAGGTGCA GCATCAGGGC CTGTCTTCCG CCTCGATGAC CGCCTCCTCC 
AAGACCATGT CCTCCTCCAC ACTGACCACT CTCGTCAAAA TAGAGGCTCC CTCGCCAAAG 
GTATCGGAAT TGGAAAAGTC CACAGGCAAC TCCGTTCCCA TTGGCATCGC CGTGGCCAGG 
AAACGCCCGC AAGAAGCTCT GGTGCCGGCT ATCAACACCC CCGCTACGCT GCCGCTGCAG 
CCACCACTCA ACAAGGACTT GAACTGCTTC GGCATACGCG TCGCTGACCT CGGTGCCACC 
AGTTGCGGCA ATTTGTACTT TACTGGCAAC GGCGACCTGA TGACCACCGG CACAGCCACC 
GCCGAGGAAC TGGCTCTGAG TGCAGCGGGT GTGAACCGGG CACCCTCCAC TTTCTGGCAG 
TATCCAAATG CATTACCCAT TGAATCAGTG ATTTCCATGT CGCCCGCCAC GGTGGGCCTG 
CAGTACTCGC GGGAGGCCAG TCGCGGACAG GTGGTGCTGC TGCCGGCCGG CCCAACAGCG 
CTCGGCATAT CAAATTTTCC CCTCTTAACG CCCACAGATC CGTTCCAGCA GGCAGCGGCT 
GCGGCGGCCT TCGTCTGGCC CTCGGCCTAC ATCCCCCAGG CGCCGGCACC TGGTGGTCAC 
TCTGCTGCGG CAGCCGCCGC AGCTGCTTCG CTGCAGCCGA CGCCCAACTT GTCCAGTTTG 
CCCAACTTCA TCTTTCCCTC GATGGGCGGC CACCAGGCGC TGAGCACAAC GCCTTCGTAC 
GCGTCCACCC TGCAGCTGTA TCTGGCCGCT GCGGCCACTG CGACGGGCAG CTCGACGATG 
TGCCAGCACA GCAATCAGCA GACCAGCACC ACAACCACCA ATTCCACCAT TAATCTGAGT 
CTGGCCGCTG GTTCGGGGGT CACGCAAAGC GGCAATGCGG CTAGCAGCTT AAGTTCCAGC 
AGTAGTCTCA GCGCGAGTAG CTCCCGTTTC CTCAGCCTGG CCACTGGTCA GACTGGGATT 
CCTTCACTTC TCTCCTTGCC GCCACCGACA GGTTTGAAAG AGGAGTACCC GATGCCGCTG 
GCATTGCCGC CCCTTGTGCC GCTCGAGGCG GCGCGGGATA AGGAGCAGGC CTTGAACCTA 
ATGCGTCTGC CCACGCCGCC CACCTCGGCC ACCATGGAGC CGTCTTCCCT AGGCCATGCA 
ACGCCCCACC ATCTCTTCCA GTCGGGTGCC ATGGCTACGC CCACGCCAGC ATTGCTCAAC 
CTATCGATGC ATGGCAACGG TGGGGAGCTG CCTGCTGCAA CGCCTCTGCC AGCGGTCAGT 
GATGAAGCGG CGCTGAACTA CAAGCTGCAT GCACCGTTGA CACCGCAGAC ACCTCCTCGC 
CTGGCGGACA TTCCTGTGTC GGGATCGACT CAGCTCTTGC CGCAAATGCA GGATGTCAAT 
ATACAGACGG ATACGCCCGT TTGCAGTGAA GACGAGAGCT TTCCGGGCTC AGCAAAGCCG 
CAGGATCCTG CTGCAGAAGC ATTTCCGCCC CCAACCCATG TCCAGCCGCT GGAACTAACG 
AAGCCCAGTG AAGCCAGCCA TACCCAGCCT ACTGAGTGCC ATACCCAGAC CGAACCCAGT 
GACATACCCA GTGCCAGCCA GGAGGAGTCA GCGGAGCAGA CTCCCCCGGA ACCCATAGAA 
ACGATGACGC AAGCCACGCA GGCAGATCAA ACGTCCCCCG AGGACCTGAC CGGTTTGGAG 
CTGCTCTCGA ACATAAGCAC CAATAGCAAA CCACTGGTGC GAGTAAAACA AGAGCCGGTG 
GAGCACATAG AGCAGCCAGC GCCGCAGCCG CAGCCTGTGA ATATAATGCC CTCAGAGCCT 
ACGCTGCCTA TGCCGCCAAT GCTGGAAATG GAGCCCACTT CAGAACGCGA ACCACTTGGA 
GGTCTCAAGC TGCTGTGTGC ACTCGCTGAG CAACGCATCC AGGAGGAGGT GGTGCAGGGC 
AGCAGTCTGT TTGCTACGCC GTCGTCGCGA ACGCCCACTC CCACGTCGCT GGCCCTGGGA 
ACGACAGCTG CGACGCCGCC CATTTTCGAG GCCAAGTCCT GTTATGCCTT TGGGCAATCG 
CAGGGTTCCG TCTTCCCCTC CTCAACATCC TCCTTTCAGA TGCCATTAAG CTCGCCCGGA 
TTCCCCTCGA TGCAGGGCAT CGAGCTGCCA ACCACGTCGG CTGGTATCGT TGCCGAACTG 
ACTCCTGTGA AGCGCAAGAA GCACAAACAT TCCAAGTCCT CAGGCAGCGA CAGCCGCAAG 
TCGGCACGTT GCAGCAAGAA AAGCAAGAAG AAGCGCCACC ACAGCAGCAG CCGCCAGCAG 
TTCTCCGCTC CGGCCGAGGA GGATCTGGAC CTGCAGGACG ATCAGCTGCA GTCGGAGCTA 
CGCAATGCCC TCCACGCCGT GGATCCCAGC TATGCGCAGC GCTTTGGCCA GGAGGTGTTT 
AGCATCATGG ACAATAGTAT GCGTATGCGA CTGGCGAAAA TAACGCGGCA GTATCGCAAG 
AAGAAACGGA AGCTGGATGA GATTTCCAAG CACAAAAAGA AGAAGAAGTG CTCCAAGCAG 
CAGCTCGAAC TGCAACAGCA GCAGGCATCG CAACAGGCGG TGCAGCCATC TTCAGCTCTG 
CAACAGCCAC AGATGACGCT GTCCAGTGTC CTGGGAACTT CATCCCCGTT GCGCGATTAC 
AAGTTCCCAA AGTTTTCGAG CAGTAACCTT CAGGCGTCTA GTTTTTTGCG ATTCCCGGAC 
AAGACGCACT CCTTCCCCCC ACCGCCGTCC CTACAGCAAT CCCAACCGGA GCCCAATCCC 
TTGCCAAGCA GCAGCTCACC GAGTACCTCG TCGTTTGTCC GCCTGGAACC CAGCGCATTG 
GACGCGGCCG TTGCCATTGC ACCAACTACC TCTTCGACTT CCACCTCCGG CTCACCATCC 
ACCAAGCAGG TGGCTTCCGC GCGGAAGCAG CGCAAGATGA AGGCGAGCAC CGTCGGCGCC 
GCTGGAGAGC AGACAGCGGC CACGGAGGCA AAGCGGCGAT TTAGCGCCAT TGATCGCGAG 
CTTCAGCTGA CCAGCGAGCA CCTGTATCGC GATGAGACAC GTGTCCTCAC GGACATGGGT 
GGTCTTTTCT ATGCGGGTGT AATGAAGCCA TTGCGGCCGC CAGATGTGTA TTCAATCACA 
TTGGATGGAG AGCGTGGCAA CAAGTCGCAT GTAATGTCGC GCGAGGATAT ACTCAAGGAT 
ACGATCCTCG AGGTAGCTCC GAAGAGCGTG GAGAGTGTAC CAGTAGGCAC GCGCCTGTGC 
GCCTACTGGA GTCAGCAGTA CCGATGCCTT TATCCAGGAC GTGCCATCGA CTCCGAGCAG 
GTCGTCGATG GAACGGCCAG CAGCGCTACG AATGCGGCTA CAACAGCGCC GGACTTCGTA 
AGCGTAGAGT TCGACGATGG CGACAGCGGC AGGATCCGCT TGCAGAACAT CCGTATGCTC 
CTCAGCGATT ATCCCATAGC AGAGTACAAC GATAATCCCC TCTACTCAGT AGGCAAGCAG 
AAGCGAAGCG CTCTGCGCGG GGGTGAAAGT GGACCTGGCG GAGTCACTCA GGACCACTTG 
ACCGTGCCCG GCTGCGAGGA TTCGCACAGC CATCATAGCC TGGGGATGAG TAGCGATAAC 
ACCACATCGC TGGCGGCAAC TATGGAGTTG TTCACGCAGC GCAGTGAGAA GAAGCGCCTG 
AAGAAGAGCC TCAAGAAGAT GTCCAAAGCT CAAAATGGCG TTAGTCCGGC CACGGCAACC 
AATGGTGGAG CTGATGCCGC TGCCTTGGGC GATGGTGTCA GTGCCGAAGA CGCCGCTCGT 
AAGCACCATA AGCACAAGAA ACGCAAGAAG CACAAGAAGC ATCACCGCAA GAATGGCAGC 
GAAGAGCAGG AGCAACAGGT AGTCCAGCAG GACTACTCTG CGGCAGGACA GGAAGCGACA 
GAGGCTCCTT CCACAGAGAT GTCATCAGCG ACCTCAAGTC GCGTTAAGGT GGAGGTCAAA 
GTGAAGACAG AGCAGCTGGA GATGGAGGAG GAGGAGTCCG CATCCAATCT CATGTCAGAG 
ATATCCGATG AGGCCAAGGG CGACGATCTG GTCGAGCATA ACAACTCCAA GGGAAGCAGC 
AAGATTGCGG CCTTCCTGCC GGAACGGCAG CTATGGGGTT GGTATGGTAC CGCCTATCGC 
AAGGCGGGCG TCAAGGGACG CGCCAGAAAG CAATTTTACA AAACAATCAA AAGGGGAAAG 
GAGACTATCA CGGTCGGGGA CAGTGCAGTA TTTCTATCGA CGGGCAGACC AGATCGACCC 
TATATCGGAC GCATTGAGTC CATGTGGGAA ACAACCACGG GCAACAAGGT GGTGCGTGTG 
GCGTGGTTCT ATCATCCGGA GGAGACGACC GGCTGTCCAA AATTGAAATT TCCGGGTGCT 
CTTTTTGAAT CGCCGCACGA GGATGAGAAC GATGTGCAGA CCATTTCGCA TCGGTGTGAG 
GTGCTGCAGT TTGGCAGTTA CTTCGAGAAG TTCGGTGCCG ATTCGAAGCA ATATCAATCC 
ATATACGATA ACAATGATAC ATATTATTTA GCCGGGCACT ATAACCCAAG GTTACAAGTG 
CTAAAACTAC AAGACGATAT TCCAACTCTC GAAGAGCTGC AGGATACAAA TACTACTACT 
ACTACTACAG AAACTACTAC CGAGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wge cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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