Efa6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Efa6
Entrez Gene ID	42665
Full Name	Exchange factor for Arf 6
Synonyms	CG18185,CG31158,CG6941,Dmel\CG31158,EFA6,dEfa6,dPsd
General protein information	Preferred Names Exchange factor for Arf 6 Names CG31158 gene product from transcript CG31158-RG CG31158-PC CG31158-PD CG31158-PG CG31158-PH CG31158-PI Efa6 pleckstrin and Sec7 domain containing Efa6-PC Efa6-PD Efa6-PG Efa6-PH Efa6-PI exchange factor for Arf 6 exchange factor for Arf 6 ortholog
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94B5-94B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001104404.2	NP_001097874.1	exchange factor for Arf 6, isoform D
NM_001104403.2	NP_001097873.1	exchange factor for Arf 6, isoform C
NM_170027.4	NP_732769.3	exchange factor for Arf 6, isoform G
NM_001170220.2	NP_001163691.2	exchange factor for Arf 6, isoform H
NM_176541.3	NP_788718.3	exchange factor for Arf 6, isoform I

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Efa6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Efa6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05715	NM_001170220.2 Latest version!	Drosophila melanogaster exchange factor for Arf 6, transcript variant H (Efa6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa08660	NM_170027.4 Latest version!	Drosophila melanogaster exchange factor for Arf 6, transcript variant G (Efa6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa08661	NM_176541.3 Latest version!	Drosophila melanogaster exchange factor for Arf 6, transcript variant I (Efa6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa08662	NM_001104404.2 Latest version!	Drosophila melanogaster exchange factor for Arf 6, transcript variant D (Efa6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa08663	NM_001104403.2 Latest version!	Drosophila melanogaster exchange factor for Arf 6, transcript variant C (Efa6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Efa6 ( NM_001170220.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163691.2 Drosophila melanogaster exchange factor for Arf 6, transcript variant H (Efa6), mRNA.

Price & Availability↑

CloneID	OFa05715
Clone ID Related Accession (Same CDS sequence)	NM_001170220.2
Accession Version	NM_001170220.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	exchange factor for Arf 6, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170220.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGAGG AACTGAAAGT GGTGCTGCGG CGCAGCGAGC AGCATTCCGG TTTCGGGTTT 
TCGCTACTCG GAACCACCGG ACCGCCACAT GTCATCTACG ACATCGTCGA GAATTCGCCG 
GCAGCCGATT GCGGAGCGGT TGAGGCCGGG GATGTCATCC TCAAAGTCAA CGGAACGGAT 
GTCCATCGCT ATACAACGAA GGAAGTTCTC AAATGCCTGC GCCTGTCGGA ACAGCTGGTG 
ACCTTGGAGC TGAAGCGAGA TCCCAAGCTC AAGGCGCGTA TCAAAGAGCA GCTGGCCAAC 
ACCCAGAGTC CGCACTATGT GGACATTGAG TCGCCGAACA TCTACGATTA CCACAGCAGC 
AGCACCAACT CCTCGCCGAA TCACCGGCCG AACGCTGGTG GCAAGGGGGC GGCGACCACG 
CCCTCGCAGA CCGGACTGCG CTACAAGTCA CCGACGCACT TGCCCTCGCT GCGCCAGAAT 
TCGTCGCCAC TGCTGGCGAG TGGATCCACC ACGACCACAA CCACCGCCAC GCATACGCAC 
AGCCACAGTC GAAACTCCTC GGCCAGCTCC ACCAAGATTA AGGTGGTGGA GACAAGCATC 
ACCACCTCGA CCACGAACGT AGTGGGCCTC ACATCGCCCA CCGGTAGTGT TGGGGGTGGT 
GTTGGTGGGG AGGCCACTTC GCCCACCTTC CGCCCCTCAC GCATTCCACA GGCGCTCACC 
AAATGTGCCG TACCCAAGCC CGTGCCCGTT CTCCATTCGC CGCAGAATAA GCGGCCACGC 
CCATCGCAGA TTCCGACAAA GGCGGCGAAC GGAAACGGGA ACGGACATAC CGCCCATCTG 
CCACCGCAAT CGTTGCAGCA TTCCAACAGC TACAGCGGCA GTCCGGTGAC TCGGCAGCGG 
TTTGCCGATA GGGAGCCGGA GCGGGAACCG GAACCGAATT CGGCGCCACC GCAGCCGGCG 
AAGGCGCCAC GCTTTGAGGC TTACATGATG ACCGGTGACC TTATTCTCAA TCTGTCCCGG 
ACACCGCAGA CCAGCAATCC GCTTCCCGCC CAGGCCAAAA AGATTGACAG TCTTCGCGAT 
TCACCCAGTC GTTTGGTAAA TCCGCGTATC AACGGCGCAC TAGCACCGCG TGCCTCTGGT 
GAATCCTCGC CCACGTCCTC CTCTTCGGTA GACTCGCCCA CCAACACCAG CTCAGATTCC 
GTGAAGCGCG AGGCAAAGCT GCTGCAGAAA CAACAGCAGC AGCAGCAACA AACCTACCAA 
CAACAACAGC AGCGGGACAG CATCAACAAC AGCTACAATC GTAAGGATTC GCTGACCAAC 
GATACGCTGC TCATGTGCGA GGAGTTGGAG CCGGACGAGG AGGGCGAGTA TGTCCTGGAA 
GAGGACAACA AACAGCAGCG GCAGCGCCAA CAGCAACAAC GATACCGCCA GCAGCAAAAT 
CAGCAACGCT ACGAATACTA CCAAAACGAG GACGAGCTGG AGGAGCAGGA GGAGGTTGAG 
GAGGAGCGCG AGGAGGATCA AACACACTAC GACATCACCA ATATTGAAAC CTACCAGAGC 
GGTGTGGGCC GGGGTGACGA CGATGACAGT GATCGGCAGT GTCTGGTAGA CGACGACGAT 
GACGATGATG CCTACGATGA TGAGGAGAAC GATGCGGGCG ACGAGGACTA CTCCACCAAC 
TCGCTGGGCT CCGGTTCAGC CAAGCAACGA TTGCGGGCGT TGAAACAGCG CACTGCCACC 
CGCCAGCAGC AGCGAAATCG GGATGCCGTC GACTGTGCAG GACGCTCCGG ATCGGGATCA 
TCATCTACCA CGGTCAAGAG CGAGGCTGGC GGCCTGGGCC TAGATGAAAC CTCCTTCTCA 
GTGCCAACCT CTCCGATCTC GCTGTCGACG CCGCTGATCG ACAAGGAGAC GGCCAACTCG 
GTGCCCACGA GCCCGGAGCC CAGTTCGCTT GTTCCGGAGT CGAGCAGTGG CGCTGGCGCC 
GGAGCCGTGG TGGTGCGCCG GCATAACGGA CATGTGGTGC GGAAGTGTGA TGCCGCCGGT 
TTCCGCACCA GCAAGTCTGA GGACCATTTG CAGCAGATCC AGCGCGAGGG CATCGCCGCT 
GTGATACCCA TCGACATTGA TGAGGATGTG AATAGTTCGC TGAACACGCT GCTGGACACG 
CGTCAGGACT CCGAGGACTC GCAGTCGATG GCCACTGTAA TTGTAAACAA CTCGTCATTG 
GCCTCGAACA ACAACGAGGG CGAACAGACC GACAACCGCA GCAGCAGCAG CAGCAGCAAC 
TCCAGCGACA ACAATAACTG CAGCAGCAAC ACCGGCGAAC CAGCAACGAG TGAAACTGCA 
ACAGCAACTG CAACAATCAT AACTGCAACA TCAACGAGAA CGATGAACTG CAGCAGCAAA 
TTAAACTATA TTTTATGCAA AAAGGCATCG GATCGCGATC GGATCGTGTG GACTTATAAT 
GCGCCACTCC AGCCGCATCA GTTGGCGGCT CTTCAGCGAC AGCAGCAACA GCAAGAGCAG 
CAATTCCAAC AGCAGCAGCA GCAGCTTCAC CAGCAACATC TGCAGCAACA GCAGCAGCTT 
CAGCAGCAAC ATCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAGCA GCAACTCTAC 
GGTCAGCAAT CGCATTCAAA TTCACATTCA AGTTCCATTA GTTCGTCGCC CCAGCACTCG 
GCTGTGGGCA GTCCGGCCTC GCCCACGTCG GTTTCCTCGT CGGTGATGTC CTCGTCGGGC 
TCCAAGGGCG CCCTGGGCCT GGGCAGCAGC AGCAATGGTC CCATGGCCGC CGTGCAGCAA 
CAACAGCTGA GAGAGAGGGA GCAGGGCGGC CAGGTCGCGC AACCGCCCAG CGGGATTCCT 
GGCTTGCTTA GCTGCCCAGG TGGTGGGTGC GGAAACAATG GTGGTGGTGG AGGCATTGGT 
GGTGGTGGCA ACAACGATCA GAGCGTCTCA GAGGCCATTT CGAATATATC TAGTCCCGAC 
TACCAAGACG ACGATAATTT ATTGAGTTCT CGCGATATTC TAGGCGGCAT GGTACTTAGC 
GATCCCAGTG ATTCGGACTC CACCATCCTC GTCTCGGACG CGGCCGCCCA TCAGCGCCAG 
CAGCTCAAGC AGCAGTTGCG CGCCCAGCAG CAACAGCAGA GAGAAAGGGA ACGGGATAGG 
GATCGAGACA GGGAACAGTC CGAGCACAAA GTGGTCATAC AAGTGCGCGG TCTGGACAGT 
AACAGCAGCG GCGGCAACGG AACTAACGGC CGCTCCGAGG AGGATGTGGT CACGCTGACG 
GACGAGCCGC TGGGCACGAT GACCGTTGGC ATGCGGGACG CCTCGCCGCC AGTCTCTGAC 
GATGGCAGCG ATGTGGAGTC CCTGCACTCG TATCACTACT CGCCCAAGGC CGTGGACATG 
CCCTCGGCCA TACGCCTGGC CAAAAGACTG CACTCCCTCG ACGGTTTCAA GAAGAGCGAT 
GTGTCGCGAC ACCTCAGCAA GAACAATGAC TTTAGTCGAG CGGTGGCCGA TGAGTATCTC 
AAACATTTTA CCTTTGAGAA GAAGTCACTT GACCAAGCGC TGCGTGAGTT CTTGCAGCAA 
TTCTCGCTGT CCGGCGAAAC GCAGGAACGG GAACGAGTGC TGGTGCACTT TTCCAAGCGC 
TTCCTCGACT GCAATCCTGG CACCTTTAAC TCGCAGGACG CCGTGCACAC GCTGACCTGT 
GCCATAATGT TGCTGAATAC GGACTTGCAC GGCCAGAACA TAAATCGTAA GATGAGCTGT 
GCGGAATTCG TCGACAACCT GGCAGATCTC AACGATGGCG AGAACTTTCC CAAGGATGTG 
CTCAAGTCGC TTTACCAGGC CATTAAGACC AAGCCGCTTG AGTGGGCACT AGATGAAGAG 
GCAGGTGATC TGCAGCAGCA AAGAGCCAAC AATAGTGCCC TGGGCAATGT GGGTCTCAAT 
CCCTTCCTCG ATCCCCCAGA ACTGGCCACA GCTGTGGAAT ACAAAAAAGG CTATGTGATG 
CGTAAATGCT GCTATGACAG CAGCTTTAAG AAGACTCCCT TTGGCAAACG CTCCTGGAAG 
ATGTTCTACT GCACGCTGCG TGATCTTGTG CTGTATTTGC ATAAGGATGA GCACGGTTTT 
CGTAAAAGTC AAATGTCCGA CAATCTGCAC AATGCAATAC GCATACATCA CGCACTGGCC 
ACCAAGGCCA ACGACTACAC CAAGAAGCAA CATGTGTTCC GGCTGCAGAC GGCCGACCAG 
GCCGAGTATC TGTTCCAGAC CAGCGACTCC AAGGAGCTGC AGTCGTGGGT GGAGACGATA 
AATTACGTGT GCGCCGCTAT ATCAGCGCCT CCGCTCGAGG GAGGGGTGGG CAGTCAGAAA 
CGATTCCAAC GTCCGCTCCT GCCCAGCAAA CAATCCAAGC TGATGCTGAA AGAGCAGTTG 
GACTCGCACG AGGTGCAGCT GGCGCAGTTG GATCAGGAGC TTAACGAGCA CAAGAAGGGT 
CCTATTCCCA GCAAGGGCTT GGCTCTGCAG AACTACAAGG AAAAGGAGAG CTACTTGCAG 
TATGAACTTC GTCGCTACCG CACTTATGTG AGCATTTTGA GCGCCAAGAT GCTGGCTGAT 
CAGCAGCAGC TGGAGCTGCA GGCGCAGCAG CCCTCTCCAG CGTCGCACGA GGAAGAGGCC 
GACACATTCC CAGTGGGCAC CACCGCCTGC ACGCCACCCA CGCCGCAAAG TATTAACCAG 
AAGGATCAGC AGAAGGAGCA GCAGCAGCAA CAGCCAACGA ACAGAAAAGA GAAGAAAAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163691.2
CDS	527..5332
Translation	MSEELKVVLRRSEQHSGFGFSLLGTTGPPHVIYDIVENSPAADCGAVEAGDVILKVNGTDVHRYTTKEVLKCLRLSEQLVTLELKRDPKLKARIKEQLANTQSPHYVDIESPNIYDYHSSSTNSSPNHRPNAGGKGAATTPSQTGLRYKSPTHLPSLRQNSSPLLASGSTTTTTTATHTHSHSRNSSASSTKIKVVETSITTSTTNVVGLTSPTGSVGGGVGGEATSPTFRPSRIPQALTKCAVPKPVPVLHSPQNKRPRPSQIPTKAANGNGNGHTAHLPPQSLQHSNSYSGSPVTRQRFADREPEREPEPNSAPPQPAKAPRFEAYMMTGDLILNLSRTPQTSNPLPAQAKKIDSLRDSPSRLVNPRINGALAPRASGESSPTSSSSVDSPTNTSSDSVKREAKLLQKQQQQQQQTYQQQQQRDSINNSYNRKDSLTNDTLLMCEELEPDEEGEYVLEEDNKQQRQRQQQQRYRQQQNQQRYEYYQNEDELEEQEEVEEEREEDQTHYDITNIETYQSGVGRGDDDDSDRQCLVDDDDDDDAYDDEENDAGDEDYSTNSLGSGSAKQRLRALKQRTATRQQQRNRDAVDCAGRSGSGSSSTTVKSEAGGLGLDETSFSVPTSPISLSTPLIDKETANSVPTSPEPSSLVPESSSGAGAGAVVVRRHNGHVVRKCDAAGFRTSKSEDHLQQIQREGIAAVIPIDIDEDVNSSLNTLLDTRQDSEDSQSMATVIVNNSSLASNNNEGEQTDNRSSSSSSNSSDNNNCSSNTGEPATSETATATATIITATSTRTMNCSSKLNYILCKKASDRDRIVWTYNAPLQPHQLAALQRQQQQQEQQFQQQQQQLHQQHLQQQQQLQQQHQQQQQQQQQQQQQQLYGQQSHSNSHSSSISSSPQHSAVGSPASPTSVSSSVMSSSGSKGALGLGSSSNGPMAAVQQQQLREREQGGQVAQPPSGIPGLLSCPGGGCGNNGGGGGIGGGGNNDQSVSEAISNISSPDYQDDDNLLSSRDILGGMVLSDPSDSDSTILVSDAAAHQRQQLKQQLRAQQQQQRERERDRDRDREQSEHKVVIQVRGLDSNSSGGNGTNGRSEEDVVTLTDEPLGTMTVGMRDASPPVSDDGSDVESLHSYHYSPKAVDMPSAIRLAKRLHSLDGFKKSDVSRHLSKNNDFSRAVADEYLKHFTFEKKSLDQALREFLQQFSLSGETQERERVLVHFSKRFLDCNPGTFNSQDAVHTLTCAIMLLNTDLHGQNINRKMSCAEFVDNLADLNDGENFPKDVLKSLYQAIKTKPLEWALDEEAGDLQQQRANNSALGNVGLNPFLDPPELATAVEYKKGYVMRKCCYDSSFKKTPFGKRSWKMFYCTLRDLVLYLHKDEHGFRKSQMSDNLHNAIRIHHALATKANDYTKKQHVFRLQTADQAEYLFQTSDSKELQSWVETINYVCAAISAPPLEGGVGSQKRFQRPLLPSKQSKLMLKEQLDSHEVQLAQLDQELNEHKKGPIPSKGLALQNYKEKESYLQYELRRYRTYVSILSAKMLADQQQLELQAQQPSPASHEEEADTFPVGTTACTPPTPQSINQKDQQKEQQQQQPTNRKEKKKK

Target ORF information:

RefSeq Version	NM_001170220.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster exchange factor for Arf 6, transcript variant H (Efa6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170220.2

ORF Insert Sequence:

ATGAGCGAGG AACTGAAAGT GGTGCTGCGG CGCAGCGAGC AGCATTCCGG TTTCGGGTTT 
TCGCTACTCG GAACCACCGG ACCGCCACAT GTCATCTACG ACATCGTCGA GAATTCGCCG 
GCAGCCGATT GCGGAGCGGT TGAGGCCGGG GATGTCATCC TCAAAGTCAA CGGAACGGAT 
GTCCATCGCT ATACAACGAA GGAAGTTCTC AAATGCCTGC GCCTGTCGGA ACAGCTGGTG 
ACCTTGGAGC TGAAGCGAGA TCCCAAGCTC AAGGCGCGTA TCAAAGAGCA GCTGGCCAAC 
ACCCAGAGTC CGCACTATGT GGACATTGAG TCGCCGAACA TCTACGATTA CCACAGCAGC 
AGCACCAACT CCTCGCCGAA TCACCGGCCG AACGCTGGTG GCAAGGGGGC GGCGACCACG 
CCCTCGCAGA CCGGACTGCG CTACAAGTCA CCGACGCACT TGCCCTCGCT GCGCCAGAAT 
TCGTCGCCAC TGCTGGCGAG TGGATCCACC ACGACCACAA CCACCGCCAC GCATACGCAC 
AGCCACAGTC GAAACTCCTC GGCCAGCTCC ACCAAGATTA AGGTGGTGGA GACAAGCATC 
ACCACCTCGA CCACGAACGT AGTGGGCCTC ACATCGCCCA CCGGTAGTGT TGGGGGTGGT 
GTTGGTGGGG AGGCCACTTC GCCCACCTTC CGCCCCTCAC GCATTCCACA GGCGCTCACC 
AAATGTGCCG TACCCAAGCC CGTGCCCGTT CTCCATTCGC CGCAGAATAA GCGGCCACGC 
CCATCGCAGA TTCCGACAAA GGCGGCGAAC GGAAACGGGA ACGGACATAC CGCCCATCTG 
CCACCGCAAT CGTTGCAGCA TTCCAACAGC TACAGCGGCA GTCCGGTGAC TCGGCAGCGG 
TTTGCCGATA GGGAGCCGGA GCGGGAACCG GAACCGAATT CGGCGCCACC GCAGCCGGCG 
AAGGCGCCAC GCTTTGAGGC TTACATGATG ACCGGTGACC TTATTCTCAA TCTGTCCCGG 
ACACCGCAGA CCAGCAATCC GCTTCCCGCC CAGGCCAAAA AGATTGACAG TCTTCGCGAT 
TCACCCAGTC GTTTGGTAAA TCCGCGTATC AACGGCGCAC TAGCACCGCG TGCCTCTGGT 
GAATCCTCGC CCACGTCCTC CTCTTCGGTA GACTCGCCCA CCAACACCAG CTCAGATTCC 
GTGAAGCGCG AGGCAAAGCT GCTGCAGAAA CAACAGCAGC AGCAGCAACA AACCTACCAA 
CAACAACAGC AGCGGGACAG CATCAACAAC AGCTACAATC GTAAGGATTC GCTGACCAAC 
GATACGCTGC TCATGTGCGA GGAGTTGGAG CCGGACGAGG AGGGCGAGTA TGTCCTGGAA 
GAGGACAACA AACAGCAGCG GCAGCGCCAA CAGCAACAAC GATACCGCCA GCAGCAAAAT 
CAGCAACGCT ACGAATACTA CCAAAACGAG GACGAGCTGG AGGAGCAGGA GGAGGTTGAG 
GAGGAGCGCG AGGAGGATCA AACACACTAC GACATCACCA ATATTGAAAC CTACCAGAGC 
GGTGTGGGCC GGGGTGACGA CGATGACAGT GATCGGCAGT GTCTGGTAGA CGACGACGAT 
GACGATGATG CCTACGATGA TGAGGAGAAC GATGCGGGCG ACGAGGACTA CTCCACCAAC 
TCGCTGGGCT CCGGTTCAGC CAAGCAACGA TTGCGGGCGT TGAAACAGCG CACTGCCACC 
CGCCAGCAGC AGCGAAATCG GGATGCCGTC GACTGTGCAG GACGCTCCGG ATCGGGATCA 
TCATCTACCA CGGTCAAGAG CGAGGCTGGC GGCCTGGGCC TAGATGAAAC CTCCTTCTCA 
GTGCCAACCT CTCCGATCTC GCTGTCGACG CCGCTGATCG ACAAGGAGAC GGCCAACTCG 
GTGCCCACGA GCCCGGAGCC CAGTTCGCTT GTTCCGGAGT CGAGCAGTGG CGCTGGCGCC 
GGAGCCGTGG TGGTGCGCCG GCATAACGGA CATGTGGTGC GGAAGTGTGA TGCCGCCGGT 
TTCCGCACCA GCAAGTCTGA GGACCATTTG CAGCAGATCC AGCGCGAGGG CATCGCCGCT 
GTGATACCCA TCGACATTGA TGAGGATGTG AATAGTTCGC TGAACACGCT GCTGGACACG 
CGTCAGGACT CCGAGGACTC GCAGTCGATG GCCACTGTAA TTGTAAACAA CTCGTCATTG 
GCCTCGAACA ACAACGAGGG CGAACAGACC GACAACCGCA GCAGCAGCAG CAGCAGCAAC 
TCCAGCGACA ACAATAACTG CAGCAGCAAC ACCGGCGAAC CAGCAACGAG TGAAACTGCA 
ACAGCAACTG CAACAATCAT AACTGCAACA TCAACGAGAA CGATGAACTG CAGCAGCAAA 
TTAAACTATA TTTTATGCAA AAAGGCATCG GATCGCGATC GGATCGTGTG GACTTATAAT 
GCGCCACTCC AGCCGCATCA GTTGGCGGCT CTTCAGCGAC AGCAGCAACA GCAAGAGCAG 
CAATTCCAAC AGCAGCAGCA GCAGCTTCAC CAGCAACATC TGCAGCAACA GCAGCAGCTT 
CAGCAGCAAC ATCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAGCA GCAACTCTAC 
GGTCAGCAAT CGCATTCAAA TTCACATTCA AGTTCCATTA GTTCGTCGCC CCAGCACTCG 
GCTGTGGGCA GTCCGGCCTC GCCCACGTCG GTTTCCTCGT CGGTGATGTC CTCGTCGGGC 
TCCAAGGGCG CCCTGGGCCT GGGCAGCAGC AGCAATGGTC CCATGGCCGC CGTGCAGCAA 
CAACAGCTGA GAGAGAGGGA GCAGGGCGGC CAGGTCGCGC AACCGCCCAG CGGGATTCCT 
GGCTTGCTTA GCTGCCCAGG TGGTGGGTGC GGAAACAATG GTGGTGGTGG AGGCATTGGT 
GGTGGTGGCA ACAACGATCA GAGCGTCTCA GAGGCCATTT CGAATATATC TAGTCCCGAC 
TACCAAGACG ACGATAATTT ATTGAGTTCT CGCGATATTC TAGGCGGCAT GGTACTTAGC 
GATCCCAGTG ATTCGGACTC CACCATCCTC GTCTCGGACG CGGCCGCCCA TCAGCGCCAG 
CAGCTCAAGC AGCAGTTGCG CGCCCAGCAG CAACAGCAGA GAGAAAGGGA ACGGGATAGG 
GATCGAGACA GGGAACAGTC CGAGCACAAA GTGGTCATAC AAGTGCGCGG TCTGGACAGT 
AACAGCAGCG GCGGCAACGG AACTAACGGC CGCTCCGAGG AGGATGTGGT CACGCTGACG 
GACGAGCCGC TGGGCACGAT GACCGTTGGC ATGCGGGACG CCTCGCCGCC AGTCTCTGAC 
GATGGCAGCG ATGTGGAGTC CCTGCACTCG TATCACTACT CGCCCAAGGC CGTGGACATG 
CCCTCGGCCA TACGCCTGGC CAAAAGACTG CACTCCCTCG ACGGTTTCAA GAAGAGCGAT 
GTGTCGCGAC ACCTCAGCAA GAACAATGAC TTTAGTCGAG CGGTGGCCGA TGAGTATCTC 
AAACATTTTA CCTTTGAGAA GAAGTCACTT GACCAAGCGC TGCGTGAGTT CTTGCAGCAA 
TTCTCGCTGT CCGGCGAAAC GCAGGAACGG GAACGAGTGC TGGTGCACTT TTCCAAGCGC 
TTCCTCGACT GCAATCCTGG CACCTTTAAC TCGCAGGACG CCGTGCACAC GCTGACCTGT 
GCCATAATGT TGCTGAATAC GGACTTGCAC GGCCAGAACA TAAATCGTAA GATGAGCTGT 
GCGGAATTCG TCGACAACCT GGCAGATCTC AACGATGGCG AGAACTTTCC CAAGGATGTG 
CTCAAGTCGC TTTACCAGGC CATTAAGACC AAGCCGCTTG AGTGGGCACT AGATGAAGAG 
GCAGGTGATC TGCAGCAGCA AAGAGCCAAC AATAGTGCCC TGGGCAATGT GGGTCTCAAT 
CCCTTCCTCG ATCCCCCAGA ACTGGCCACA GCTGTGGAAT ACAAAAAAGG CTATGTGATG 
CGTAAATGCT GCTATGACAG CAGCTTTAAG AAGACTCCCT TTGGCAAACG CTCCTGGAAG 
ATGTTCTACT GCACGCTGCG TGATCTTGTG CTGTATTTGC ATAAGGATGA GCACGGTTTT 
CGTAAAAGTC AAATGTCCGA CAATCTGCAC AATGCAATAC GCATACATCA CGCACTGGCC 
ACCAAGGCCA ACGACTACAC CAAGAAGCAA CATGTGTTCC GGCTGCAGAC GGCCGACCAG 
GCCGAGTATC TGTTCCAGAC CAGCGACTCC AAGGAGCTGC AGTCGTGGGT GGAGACGATA 
AATTACGTGT GCGCCGCTAT ATCAGCGCCT CCGCTCGAGG GAGGGGTGGG CAGTCAGAAA 
CGATTCCAAC GTCCGCTCCT GCCCAGCAAA CAATCCAAGC TGATGCTGAA AGAGCAGTTG 
GACTCGCACG AGGTGCAGCT GGCGCAGTTG GATCAGGAGC TTAACGAGCA CAAGAAGGGT 
CCTATTCCCA GCAAGGGCTT GGCTCTGCAG AACTACAAGG AAAAGGAGAG CTACTTGCAG 
TATGAACTTC GTCGCTACCG CACTTATGTG AGCATTTTGA GCGCCAAGAT GCTGGCTGAT 
CAGCAGCAGC TGGAGCTGCA GGCGCAGCAG CCCTCTCCAG CGTCGCACGA GGAAGAGGCC 
GACACATTCC CAGTGGGCAC CACCGCCTGC ACGCCACCCA CGCCGCAAAG TATTAACCAG 
AAGGATCAGC AGAAGGAGCA GCAGCAGCAA CAGCCAACGA ACAGAAAAGA GAAGAAAAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Efa6 ( NM_170027.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732769.3 Drosophila melanogaster exchange factor for Arf 6, transcript variant G (Efa6), mRNA.

Price & Availability↑

CloneID	OFa08660
Clone ID Related Accession (Same CDS sequence)	NM_170027.4
Accession Version	NM_170027.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	exchange factor for Arf 6, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_170027.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGAGG AACTGAAAGT GGTGCTGCGG CGCAGCGAGC AGCATTCCGG TTTCGGGTTT 
TCGCTACTCG GAACCACCGG ACCGCCACAT GTCATCTACG ACATCGTCGA GAATTCGCCG 
GCAGCCGATT GCGGAGCGGT TGAGGCCGGG GATGTCATCC TCAAAGTCAA CGGAACGGAT 
GTCCATCGCT ATACAACGAA GGAAGTTCTC AAATGCCTGC GCCTGTCGGA ACAGCTGGTG 
ACCTTGGAGC TGAAGCGAGA TCCCAAGCTC AAGGCGCGTA TCAAAGAGCA GCTGGCCAAC 
ACCCAGAGTC CGCACTATGT GGACATTGAG TCGCCGAACA TCTACGATTA CCACAGCAGC 
AGCACCAACT CCTCGCCGAA TCACCGGCCG AACGCTGGTG GCAAGGGGGC GGCGACCACG 
CCCTCGCAGA CCGGACTGCG CTACAAGTCA CCGACGCACT TGCCCTCGCT GCGCCAGAAT 
TCGTCGCCAC TGCTGGCGAG TGGATCCACC ACGACCACAA CCACCGCCAC GCATACGCAC 
AGCCACAGTC GAAACTCCTC GGCCAGCTCC ACCAAGATTA AGGTGGTGGA GACAAGCATC 
ACCACCTCGA CCACGAACGT AGTGGGCCTC ACATCGCCCA CCGGTAGTGT TGGGGGTGGT 
GTTGGTGGGG AGGCCACTTC GCCCACCTTC CGCCCCTCAC GCATTCCACA GGCGCTCACC 
AAATGTGCCG TACCCAAGCC CGTGCCCGTT CTCCATTCGC CGCAGAATAA GCGGCCACGC 
CCATCGCAGA TTCCGACAAA GGCGGCGAAC GGAAACGGGA ACGGACATAC CGCCCATCTG 
CCACCGCAAT CGTTGCAGCA TTCCAACAGC TACAGCGGCA GTCCGGTGAC TCGGCAGCGG 
TTTGCCGATA GGGAGCCGGA GCGGGAACCG GAACCGAATT CGGCGCCACC GCAGCCGGCG 
AAGGCGCCAC GCTTTGAGGC TTACATGATG ACCGGTGACC TTATTCTCAA TCTGTCCCGG 
ACACCGCAGA CCAGCAATCC GCTTCCCGCC CAGGCCAAAA AGATTGACAG TCTTCGCGAT 
TCACCCAGTC GTTTGGTAAA TCCGCGTATC AACGGCGCAC TAGCACCGCG TGCCTCTGGT 
GAATCCTCGC CCACGTCCTC CTCTTCGGTA GACTCGCCCA CCAACACCAG CTCAGATTCC 
GTGAAGCGCG AGGCAAAGCT GCTGCAGAAA CAACAGCAGC AGCAGCAACA AACCTACCAA 
CAACAACAGC AGCGGGACAG CATCAACAAC AGCTACAATC GTAAGGATTC GCTGACCAAC 
GATACGCTGC TCATGTGCGA GGAGTTGGAG CCGGACGAGG AGGGCGAGTA TGTCCTGGAA 
GAGGACAACA AACAGCAGCG GCAGCGCCAA CAGCAACAAC GATACCGCCA GCAGCAAAAT 
CAGCAACGCT ACGAATACTA CCAAAACGAG GACGAGCTGG AGGAGCAGGA GGAGGTTGAG 
GAGGAGCGCG AGGAGGATCA AACACACTAC GACATCACCA ATATTGAAAC CTACCAGAGC 
GGTGTGGGCC GGGGTGACGA CGATGACAGT GATCGGCAGT GTCTGGTAGA CGACGACGAT 
GACGATGATG CCTACGATGA TGAGGAGAAC GATGCGGGCG ACGAGGACTA CTCCACCAAC 
TCGCTGGGCT CCGGTTCAGC CAAGCAACGA TTGCGGGCGT TGAAACAGCG CACTGCCACC 
CGCCAGCAGC AGCGAAATCG GGATGCCGTC GACTGTGCAG GACGCTCCGG ATCGGGATCA 
TCATCTACCA CGGTCAAGAG CGAGGCTGGC GGCCTGGGCC TAGATGAAAC CTCCTTCTCA 
GTGCCAACCT CTCCGATCTC GCTGTCGACG CCGCTGATCG ACAAGGAGAC GGCCAACTCG 
GTGCCCACGA GCCCGGAGCC CAGTTCGCTT GTTCCGGAGT CGAGCAGTGG CGCTGGCGCC 
GGAGCCGTGG TGGTGCGCCG GCATAACGGA CATGTGGTGC GGAAGTGTGA TGCCGCCGGT 
TTCCGCACCA GCAAGTCTGA GGACCATTTG CAGCAGATCC AGCGCGAGGG CATCGCCGCT 
GTGATACCCA TCGACATTGA TGAGGATGTG AATAGTTCGC TGAACACGCT GCTGGACACG 
CGTCAGGACT CCGAGGACTC GCAGGCATCG GATCGCGATC GGATCGTGTG GACTTATAAT 
GCGCCACTCC AGCCGCATCA GTTGGCGGCT CTTCAGCGAC AGCAGCAACA GCAAGAGCAG 
CAATTCCAAC AGCAGCAGCA GCAGCTTCAC CAGCAACATC TGCAGCAACA GCAGCAGCTT 
CAGCAGCAAC ATCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAGCA GCAACTCTAC 
GGCGGCATGG TACTTAGCGA TCCCAGTGAT TCGGACTCCA CCATCCTCGT CTCGGACGCG 
GCCGCCCATC AGCGCCAGCA GCTCAAGCAG CAGTTGCGCG CCCAGCAGCA ACAGCAGAGA 
GAAAGGGAAC GGGATAGGGA TCGAGACAGG GAACAGTCCG AGCACAAAGT GGTCATACAA 
GTGCGCGGTC TGGACAGTAA CAGCAGCGGC GGCAACGGAA CTAACGGCCG CTCCGAGGAG 
GATGTGGTCA CGCTGACGGA CGAGCCGCTG GGCACGATGA CCGTTGGCAT GCGGGACGCC 
TCGCCGCCAG TCTCTGACGA TGGCAGCGAT GTGGAGTCCC TGCACTCGTA TCACTACTCG 
CCCAAGGCCG TGGACATGCC CTCGGCCATA CGCCTGGCCA AAAGACTGCA CTCCCTCGAC 
GGTTTCAAGA AGAGCGATGT GTCGCGACAC CTCAGCAAGA ACAATGACTT TAGTCGAGCG 
GTGGCCGATG AGTATCTCAA ACATTTTACC TTTGAGAAGA AGTCACTTGA CCAAGCGCTG 
CGTGAGTTCT TGCAGCAATT CTCGCTGTCC GGCGAAACGC AGGAACGGGA ACGAGTGCTG 
GTGCACTTTT CCAAGCGCTT CCTCGACTGC AATCCTGGCA CCTTTAACTC GCAGGACGCC 
GTGCACACGC TGACCTGTGC CATAATGTTG CTGAATACGG ACTTGCACGG CCAGAACATA 
AATCGTAAGA TGAGCTGTGC GGAATTCGTC GACAACCTGG CAGATCTCAA CGATGGCGAG 
AACTTTCCCA AGGATGTGCT CAAGTCGCTT TACCAGGCCA TTAAGACCAA GCCGCTTGAG 
TGGGCACTAG ATGAAGAGGC AGGTGATCTG CAGCAGCAAA GAGCCAACAA TAGTGCCCTG 
GGCAATGTGG GTCTCAATCC CTTCCTCGAT CCCCCAGAAC TGGCCACAGC TGTGGAATAC 
AAAAAAGGCT ATGTGATGCG TAAATGCTGC TATGACAGCA GCTTTAAGAA GACTCCCTTT 
GGCAAACGCT CCTGGAAGAT GTTCTACTGC ACGCTGCGTG ATCTTGTGCT GTATTTGCAT 
AAGGATGAGC ACGGTTTTCG TAAAAGTCAA ATGTCCGACA ATCTGCACAA TGCAATACGC 
ATACATCACG CACTGGCCAC CAAGGCCAAC GACTACACCA AGAAGCAACA TGTGTTCCGG 
CTGCAGACGG CCGACCAGGC CGAGTATCTG TTCCAGACCA GCGACTCCAA GGAGCTGCAG 
TCGTGGGTGG AGACGATAAA TTACGTGTGC GCCGCTATAT CAGCGCCTCC GCTCGAGGGA 
GGGGTGGGCA GTCAGAAACG ATTCCAACGT CCGCTCCTGC CCAGCAAACA ATCCAAGCTG 
ATGCTGAAAG AGCAGTTGGA CTCGCACGAG GTGCAGCTGG CGCAGTTGGA TCAGGAGCTT 
AACGAGCACA AGAAGGGTCC TATTCCCAGC AAGGGCTTGG CTCTGCAGAA CTACAAGGAA 
AAGGAGAGCT ACTTGCAGTA TGAACTTCGT CGCTACCGCA CTTATGTGAG CATTTTGAGC 
GCCAAGATGC TGGCTGATCA GCAGCAGCTG GAGCTGCAGG CGCAGCAGCC CTCTCCAGCG 
TCGCACGAGG AAGAGGCCGA CACATTCCCA GTGGGCACCA CCGCCTGCAC GCCACCCACG 
CCGCAAAGTA TTAACCAGAA GGATCAGCAG AAGGAGCAGC AGCAGCAACA GCCAACGAAC 
AGATGGTTCG ATGTCTTCTG CTGCTGTTGC CCACTCTGGC GGCACTTGAT CCATTCGAAA 
AGCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732769.3
CDS	527..4735
Translation	MSEELKVVLRRSEQHSGFGFSLLGTTGPPHVIYDIVENSPAADCGAVEAGDVILKVNGTDVHRYTTKEVLKCLRLSEQLVTLELKRDPKLKARIKEQLANTQSPHYVDIESPNIYDYHSSSTNSSPNHRPNAGGKGAATTPSQTGLRYKSPTHLPSLRQNSSPLLASGSTTTTTTATHTHSHSRNSSASSTKIKVVETSITTSTTNVVGLTSPTGSVGGGVGGEATSPTFRPSRIPQALTKCAVPKPVPVLHSPQNKRPRPSQIPTKAANGNGNGHTAHLPPQSLQHSNSYSGSPVTRQRFADREPEREPEPNSAPPQPAKAPRFEAYMMTGDLILNLSRTPQTSNPLPAQAKKIDSLRDSPSRLVNPRINGALAPRASGESSPTSSSSVDSPTNTSSDSVKREAKLLQKQQQQQQQTYQQQQQRDSINNSYNRKDSLTNDTLLMCEELEPDEEGEYVLEEDNKQQRQRQQQQRYRQQQNQQRYEYYQNEDELEEQEEVEEEREEDQTHYDITNIETYQSGVGRGDDDDSDRQCLVDDDDDDDAYDDEENDAGDEDYSTNSLGSGSAKQRLRALKQRTATRQQQRNRDAVDCAGRSGSGSSSTTVKSEAGGLGLDETSFSVPTSPISLSTPLIDKETANSVPTSPEPSSLVPESSSGAGAGAVVVRRHNGHVVRKCDAAGFRTSKSEDHLQQIQREGIAAVIPIDIDEDVNSSLNTLLDTRQDSEDSQASDRDRIVWTYNAPLQPHQLAALQRQQQQQEQQFQQQQQQLHQQHLQQQQQLQQQHQQQQQQQQQQQQQQLYGGMVLSDPSDSDSTILVSDAAAHQRQQLKQQLRAQQQQQRERERDRDRDREQSEHKVVIQVRGLDSNSSGGNGTNGRSEEDVVTLTDEPLGTMTVGMRDASPPVSDDGSDVESLHSYHYSPKAVDMPSAIRLAKRLHSLDGFKKSDVSRHLSKNNDFSRAVADEYLKHFTFEKKSLDQALREFLQQFSLSGETQERERVLVHFSKRFLDCNPGTFNSQDAVHTLTCAIMLLNTDLHGQNINRKMSCAEFVDNLADLNDGENFPKDVLKSLYQAIKTKPLEWALDEEAGDLQQQRANNSALGNVGLNPFLDPPELATAVEYKKGYVMRKCCYDSSFKKTPFGKRSWKMFYCTLRDLVLYLHKDEHGFRKSQMSDNLHNAIRIHHALATKANDYTKKQHVFRLQTADQAEYLFQTSDSKELQSWVETINYVCAAISAPPLEGGVGSQKRFQRPLLPSKQSKLMLKEQLDSHEVQLAQLDQELNEHKKGPIPSKGLALQNYKEKESYLQYELRRYRTYVSILSAKMLADQQQLELQAQQPSPASHEEEADTFPVGTTACTPPTPQSINQKDQQKEQQQQQPTNRWFDVFCCCCPLWRHLIHSKSH

Target ORF information:

RefSeq Version	NM_170027.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster exchange factor for Arf 6, transcript variant G (Efa6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170027.4

ORF Insert Sequence:

ATGAGCGAGG AACTGAAAGT GGTGCTGCGG CGCAGCGAGC AGCATTCCGG TTTCGGGTTT 
TCGCTACTCG GAACCACCGG ACCGCCACAT GTCATCTACG ACATCGTCGA GAATTCGCCG 
GCAGCCGATT GCGGAGCGGT TGAGGCCGGG GATGTCATCC TCAAAGTCAA CGGAACGGAT 
GTCCATCGCT ATACAACGAA GGAAGTTCTC AAATGCCTGC GCCTGTCGGA ACAGCTGGTG 
ACCTTGGAGC TGAAGCGAGA TCCCAAGCTC AAGGCGCGTA TCAAAGAGCA GCTGGCCAAC 
ACCCAGAGTC CGCACTATGT GGACATTGAG TCGCCGAACA TCTACGATTA CCACAGCAGC 
AGCACCAACT CCTCGCCGAA TCACCGGCCG AACGCTGGTG GCAAGGGGGC GGCGACCACG 
CCCTCGCAGA CCGGACTGCG CTACAAGTCA CCGACGCACT TGCCCTCGCT GCGCCAGAAT 
TCGTCGCCAC TGCTGGCGAG TGGATCCACC ACGACCACAA CCACCGCCAC GCATACGCAC 
AGCCACAGTC GAAACTCCTC GGCCAGCTCC ACCAAGATTA AGGTGGTGGA GACAAGCATC 
ACCACCTCGA CCACGAACGT AGTGGGCCTC ACATCGCCCA CCGGTAGTGT TGGGGGTGGT 
GTTGGTGGGG AGGCCACTTC GCCCACCTTC CGCCCCTCAC GCATTCCACA GGCGCTCACC 
AAATGTGCCG TACCCAAGCC CGTGCCCGTT CTCCATTCGC CGCAGAATAA GCGGCCACGC 
CCATCGCAGA TTCCGACAAA GGCGGCGAAC GGAAACGGGA ACGGACATAC CGCCCATCTG 
CCACCGCAAT CGTTGCAGCA TTCCAACAGC TACAGCGGCA GTCCGGTGAC TCGGCAGCGG 
TTTGCCGATA GGGAGCCGGA GCGGGAACCG GAACCGAATT CGGCGCCACC GCAGCCGGCG 
AAGGCGCCAC GCTTTGAGGC TTACATGATG ACCGGTGACC TTATTCTCAA TCTGTCCCGG 
ACACCGCAGA CCAGCAATCC GCTTCCCGCC CAGGCCAAAA AGATTGACAG TCTTCGCGAT 
TCACCCAGTC GTTTGGTAAA TCCGCGTATC AACGGCGCAC TAGCACCGCG TGCCTCTGGT 
GAATCCTCGC CCACGTCCTC CTCTTCGGTA GACTCGCCCA CCAACACCAG CTCAGATTCC 
GTGAAGCGCG AGGCAAAGCT GCTGCAGAAA CAACAGCAGC AGCAGCAACA AACCTACCAA 
CAACAACAGC AGCGGGACAG CATCAACAAC AGCTACAATC GTAAGGATTC GCTGACCAAC 
GATACGCTGC TCATGTGCGA GGAGTTGGAG CCGGACGAGG AGGGCGAGTA TGTCCTGGAA 
GAGGACAACA AACAGCAGCG GCAGCGCCAA CAGCAACAAC GATACCGCCA GCAGCAAAAT 
CAGCAACGCT ACGAATACTA CCAAAACGAG GACGAGCTGG AGGAGCAGGA GGAGGTTGAG 
GAGGAGCGCG AGGAGGATCA AACACACTAC GACATCACCA ATATTGAAAC CTACCAGAGC 
GGTGTGGGCC GGGGTGACGA CGATGACAGT GATCGGCAGT GTCTGGTAGA CGACGACGAT 
GACGATGATG CCTACGATGA TGAGGAGAAC GATGCGGGCG ACGAGGACTA CTCCACCAAC 
TCGCTGGGCT CCGGTTCAGC CAAGCAACGA TTGCGGGCGT TGAAACAGCG CACTGCCACC 
CGCCAGCAGC AGCGAAATCG GGATGCCGTC GACTGTGCAG GACGCTCCGG ATCGGGATCA 
TCATCTACCA CGGTCAAGAG CGAGGCTGGC GGCCTGGGCC TAGATGAAAC CTCCTTCTCA 
GTGCCAACCT CTCCGATCTC GCTGTCGACG CCGCTGATCG ACAAGGAGAC GGCCAACTCG 
GTGCCCACGA GCCCGGAGCC CAGTTCGCTT GTTCCGGAGT CGAGCAGTGG CGCTGGCGCC 
GGAGCCGTGG TGGTGCGCCG GCATAACGGA CATGTGGTGC GGAAGTGTGA TGCCGCCGGT 
TTCCGCACCA GCAAGTCTGA GGACCATTTG CAGCAGATCC AGCGCGAGGG CATCGCCGCT 
GTGATACCCA TCGACATTGA TGAGGATGTG AATAGTTCGC TGAACACGCT GCTGGACACG 
CGTCAGGACT CCGAGGACTC GCAGGCATCG GATCGCGATC GGATCGTGTG GACTTATAAT 
GCGCCACTCC AGCCGCATCA GTTGGCGGCT CTTCAGCGAC AGCAGCAACA GCAAGAGCAG 
CAATTCCAAC AGCAGCAGCA GCAGCTTCAC CAGCAACATC TGCAGCAACA GCAGCAGCTT 
CAGCAGCAAC ATCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAGCA GCAACTCTAC 
GGCGGCATGG TACTTAGCGA TCCCAGTGAT TCGGACTCCA CCATCCTCGT CTCGGACGCG 
GCCGCCCATC AGCGCCAGCA GCTCAAGCAG CAGTTGCGCG CCCAGCAGCA ACAGCAGAGA 
GAAAGGGAAC GGGATAGGGA TCGAGACAGG GAACAGTCCG AGCACAAAGT GGTCATACAA 
GTGCGCGGTC TGGACAGTAA CAGCAGCGGC GGCAACGGAA CTAACGGCCG CTCCGAGGAG 
GATGTGGTCA CGCTGACGGA CGAGCCGCTG GGCACGATGA CCGTTGGCAT GCGGGACGCC 
TCGCCGCCAG TCTCTGACGA TGGCAGCGAT GTGGAGTCCC TGCACTCGTA TCACTACTCG 
CCCAAGGCCG TGGACATGCC CTCGGCCATA CGCCTGGCCA AAAGACTGCA CTCCCTCGAC 
GGTTTCAAGA AGAGCGATGT GTCGCGACAC CTCAGCAAGA ACAATGACTT TAGTCGAGCG 
GTGGCCGATG AGTATCTCAA ACATTTTACC TTTGAGAAGA AGTCACTTGA CCAAGCGCTG 
CGTGAGTTCT TGCAGCAATT CTCGCTGTCC GGCGAAACGC AGGAACGGGA ACGAGTGCTG 
GTGCACTTTT CCAAGCGCTT CCTCGACTGC AATCCTGGCA CCTTTAACTC GCAGGACGCC 
GTGCACACGC TGACCTGTGC CATAATGTTG CTGAATACGG ACTTGCACGG CCAGAACATA 
AATCGTAAGA TGAGCTGTGC GGAATTCGTC GACAACCTGG CAGATCTCAA CGATGGCGAG 
AACTTTCCCA AGGATGTGCT CAAGTCGCTT TACCAGGCCA TTAAGACCAA GCCGCTTGAG 
TGGGCACTAG ATGAAGAGGC AGGTGATCTG CAGCAGCAAA GAGCCAACAA TAGTGCCCTG 
GGCAATGTGG GTCTCAATCC CTTCCTCGAT CCCCCAGAAC TGGCCACAGC TGTGGAATAC 
AAAAAAGGCT ATGTGATGCG TAAATGCTGC TATGACAGCA GCTTTAAGAA GACTCCCTTT 
GGCAAACGCT CCTGGAAGAT GTTCTACTGC ACGCTGCGTG ATCTTGTGCT GTATTTGCAT 
AAGGATGAGC ACGGTTTTCG TAAAAGTCAA ATGTCCGACA ATCTGCACAA TGCAATACGC 
ATACATCACG CACTGGCCAC CAAGGCCAAC GACTACACCA AGAAGCAACA TGTGTTCCGG 
CTGCAGACGG CCGACCAGGC CGAGTATCTG TTCCAGACCA GCGACTCCAA GGAGCTGCAG 
TCGTGGGTGG AGACGATAAA TTACGTGTGC GCCGCTATAT CAGCGCCTCC GCTCGAGGGA 
GGGGTGGGCA GTCAGAAACG ATTCCAACGT CCGCTCCTGC CCAGCAAACA ATCCAAGCTG 
ATGCTGAAAG AGCAGTTGGA CTCGCACGAG GTGCAGCTGG CGCAGTTGGA TCAGGAGCTT 
AACGAGCACA AGAAGGGTCC TATTCCCAGC AAGGGCTTGG CTCTGCAGAA CTACAAGGAA 
AAGGAGAGCT ACTTGCAGTA TGAACTTCGT CGCTACCGCA CTTATGTGAG CATTTTGAGC 
GCCAAGATGC TGGCTGATCA GCAGCAGCTG GAGCTGCAGG CGCAGCAGCC CTCTCCAGCG 
TCGCACGAGG AAGAGGCCGA CACATTCCCA GTGGGCACCA CCGCCTGCAC GCCACCCACG 
CCGCAAAGTA TTAACCAGAA GGATCAGCAG AAGGAGCAGC AGCAGCAACA GCCAACGAAC 
AGATGGTTCG ATGTCTTCTG CTGCTGTTGC CCACTCTGGC GGCACTTGAT CCATTCGAAA 
AGCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Efa6 ( NM_176541.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788718.3 Drosophila melanogaster exchange factor for Arf 6, transcript variant I (Efa6), mRNA.

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CloneID	OFa08661
Clone ID Related Accession (Same CDS sequence)	NM_176541.3
Accession Version	NM_176541.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	exchange factor for Arf 6, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176541.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGAGG AACTGAAAGT GGTGCTGCGG CGCAGCGAGC AGCATTCCGG TTTCGGGTTT 
TCGCTACTCG GAACCACCGG ACCGCCACAT GTCATCTACG ACATCGTCGA GAATTCGCCG 
GCAGCCGATT GCGGAGCGGT TGAGGCCGGG GATGTCATCC TCAAAGTCAA CGGAACGGAT 
GTCCATCGCT ATACAACGAA GGAAGTTCTC AAATGCCTGC GCCTGTCGGA ACAGCTGGTG 
ACCTTGGAGC TGAAGCGAGA TCCCAAGCTC AAGGCGCGTA TCAAAGAGCA GCTGGCCAAC 
ACCCAGAGTC CGCACTATGT GGACATTGAG TCGCCGAACA TCTACGATTA CCACAGCAGC 
AGCACCAACT CCTCGCCGAA TCACCGGCCG AACGCTGGTG GCAAGGGGGC GGCGACCACG 
CCCTCGCAGA CCGGACTGCG CTACAAGTCA CCGACGCACT TGCCCTCGCT GCGCCAGAAT 
TCGTCGCCAC TGCTGGCGAG TGGATCCACC ACGACCACAA CCACCGCCAC GCATACGCAC 
AGCCACAGTC GAAACTCCTC GGCCAGCTCC ACCAAGATTA AGGTGGTGGA GACAAGCATC 
ACCACCTCGA CCACGAACGT AGTGGGCCTC ACATCGCCCA CCGGTAGTGT TGGGGGTGGT 
GTTGGTGGGG AGGCCACTTC GCCCACCTTC CGCCCCTCAC GCATTCCACA GGCGCTCACC 
AAATGTGCCG TACCCAAGCC CGTGCCCGTT CTCCATTCGC CGCAGAATAA GCGGCCACGC 
CCATCGCAGA TTCCGACAAA GGCGGCGAAC GGAAACGGGA ACGGACATAC CGCCCATCTG 
CCACCGCAAT CGTTGCAGCA TTCCAACAGC TACAGCGGCA GTCCGGTGAC TCGGCAGCGG 
TTTGCCGATA GGGAGCCGGA GCGGGAACCG GAACCGAATT CGGCGCCACC GCAGCCGGCG 
AAGGCGCCAC GCTTTGAGGC TTACATGATG ACCGGTGACC TTATTCTCAA TCTGTCCCGG 
ACACCGCAGA CCAGCAATCC GCTTCCCGCC CAGGCCAAAA AGATTGACAG TCTTCGCGAT 
TCACCCAGTC GTTTGGTAAA TCCGCGTATC AACGGCGCAC TAGCACCGCG TGCCTCTGGT 
GAATCCTCGC CCACGTCCTC CTCTTCGGTA GACTCGCCCA CCAACACCAG CTCAGATTCC 
GTGAAGCGCG AGGCAAAGCT GCTGCAGAAA CAACAGCAGC AGCAGCAACA AACCTACCAA 
CAACAACAGC AGCGGGACAG CATCAACAAC AGCTACAATC GTAAGGATTC GCTGACCAAC 
GATACGCTGC TCATGTGCGA GGAGTTGGAG CCGGACGAGG AGGGCGAGTA TGTCCTGGAA 
GAGGACAACA AACAGCAGCG GCAGCGCCAA CAGCAACAAC GATACCGCCA GCAGCAAAAT 
CAGCAACGCT ACGAATACTA CCAAAACGAG GACGAGCTGG AGGAGCAGGA GGAGGTTGAG 
GAGGAGCGCG AGGAGGATCA AACACACTAC GACATCACCA ATATTGAAAC CTACCAGAGC 
GGTGTGGGCC GGGGTGACGA CGATGACAGT GATCGGCAGT GTCTGGTAGA CGACGACGAT 
GACGATGATG CCTACGATGA TGAGGAGAAC GATGCGGGCG ACGAGGACTA CTCCACCAAC 
TCGCTGGGCT CCGGTTCAGC CAAGCAACGA TTGCGGGCGT TGAAACAGCG CACTGCCACC 
CGCCAGCAGC AGCGAAATCG GGATGCCGTC GACTGTGCAG GACGCTCCGG ATCGGGATCA 
TCATCTACCA CGGTCAAGAG CGAGGCTGGC GGCCTGGGCC TAGATGAAAC CTCCTTCTCA 
GTGCCAACCT CTCCGATCTC GCTGTCGACG CCGCTGATCG ACAAGGAGAC GGCCAACTCG 
GTGCCCACGA GCCCGGAGCC CAGTTCGCTT GTTCCGGAGT CGAGCAGTGG CGCTGGCGCC 
GGAGCCGTGG TGGTGCGCCG GCATAACGGA CATGTGGTGC GGAAGTGTGA TGCCGCCGGT 
TTCCGCACCA GCAAGTCTGA GGACCATTTG CAGCAGATCC AGCGCGAGGG CATCGCCGCT 
GTGATACCCA TCGACATTGA TGAGGATGTG AATAGTTCGC TGAACACGCT GCTGGACACG 
CGTCAGGACT CCGAGGACTC GCAGGCATCG GATCGCGATC GGATCGTGTG GACTTATAAT 
GCGCCACTCC AGCCGCATCA GTTGGCGGCT CTTCAGCGAC AGCAGCAACA GCAAGAGCAG 
CAATTCCAAC AGCAGCAGCA GCAGCTTCAC CAGCAACATC TGCAGCAACA GCAGCAGCTT 
CAGCAGCAAC ATCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAGCA GCAACTCTAC 
GGTCAGCAAT CGCATTCAAA TTCACATTCA AGTTCCATTA GTTCGTCGCC CCAGCACTCG 
GCTGTGGGCA GTCCGGCCTC GCCCACGTCG GTTTCCTCGT CGGTGATGTC CTCGTCGGGC 
TCCAAGGGCG CCCTGGGCCT GGGCAGCAGC AGCAATGGTC CCATGGCCGC CGTGCAGCAA 
CAACAGCTGA GAGAGAGGGA GCAGGGCGGC CAGGTCGCGC AACCGCCCAG CGGGATTCCT 
GGCTTGCTTA GCTGCCCAGG TGGTGGGTGC GGAAACAATG GTGGTGGTGG AGGCATTGGT 
GGTGGTGGCA ACAACGATCA GAGCGTCTCA GAGGCCATTT CGAATATATC TAGTCCCGAC 
TACCAAGACG ACGATAATTT ATTGAGTTCT CGCGATATTC TAGGCGGCAT GGTACTTAGC 
GATCCCAGTG ATTCGGACTC CACCATCCTC GTCTCGGACG CGGCCGCCCA TCAGCGCCAG 
CAGCTCAAGC AGCAGTTGCG CGCCCAGCAG CAACAGCAGA GAGAAAGGGA ACGGGATAGG 
GATCGAGACA GGGAACAGTC CGAGCACAAA GTGGTCATAC AAGTGCGCGG TCTGGACAGT 
AACAGCAGCG GCGGCAACGG AACTAACGGC CGCTCCGAGG AGGATGTGGT CACGCTGACG 
GACGAGCCGC TGGGCACGAT GACCGTTGGC ATGCGGGACG CCTCGCCGCC AGTCTCTGAC 
GATGGCAGCG ATGTGGAGTC CCTGCACTCG TATCACTACT CGCCCAAGGC CGTGGACATG 
CCCTCGGCCA TACGCCTGGC CAAAAGACTG CACTCCCTCG ACGGTTTCAA GAAGAGCGAT 
GTGTCGCGAC ACCTCAGCAA GAACAATGAC TTTAGTCGAG CGGTGGCCGA TGAGTATCTC 
AAACATTTTA CCTTTGAGAA GAAGTCACTT GACCAAGCGC TGCGTGAGTT CTTGCAGCAA 
TTCTCGCTGT CCGGCGAAAC GCAGGAACGG GAACGAGTGC TGGTGCACTT TTCCAAGCGC 
TTCCTCGACT GCAATCCTGG CACCTTTAAC TCGCAGGACG CCGTGCACAC GCTGACCTGT 
GCCATAATGT TGCTGAATAC GGACTTGCAC GGCCAGAACA TAAATCGTAA GATGAGCTGT 
GCGGAATTCG TCGACAACCT GGCAGATCTC AACGATGGCG AGAACTTTCC CAAGGATGTG 
CTCAAGTCGC TTTACCAGGC CATTAAGACC AAGCCGCTTG AGTGGGCACT AGATGAAGAG 
GCAGGTGATC TGCAGCAGCA AAGAGCCAAC AATAGTGCCC TGGGCAATGT GGGTCTCAAT 
CCCTTCCTCG ATCCCCCAGA ACTGGCCACA GCTGTGGAAT ACAAAAAAGG CTATGTGATG 
CGTAAATGCT GCTATGACAG CAGCTTTAAG AAGACTCCCT TTGGCAAACG CTCCTGGAAG 
ATGTTCTACT GCACGCTGCG TGATCTTGTG CTGTATTTGC ATAAGGATGA GCACGGTTTT 
CGTAAAAGTC AAATGTCCGA CAATCTGCAC AATGCAATAC GCATACATCA CGCACTGGCC 
ACCAAGGCCA ACGACTACAC CAAGAAGCAA CATGTGTTCC GGCTGCAGAC GGCCGACCAG 
GCCGAGTATC TGTTCCAGAC CAGCGACTCC AAGGAGCTGC AGTCGTGGGT GGAGACGATA 
AATTACGTGT GCGCCGCTAT ATCAGCGCCT CCGCTCGAGG GAGGGGTGGG CAGTCAGAAA 
CGATTCCAAC GTCCGCTCCT GCCCAGCAAA CAATCCAAGC TGATGCTGAA AGAGCAGTTG 
GACTCGCACG AGGTGCAGCT GGCGCAGTTG GATCAGGAGC TTAACGAGCA CAAGAAGGGT 
CCTATTCCCA GCAAGGGCTT GGCTCTGCAG AACTACAAGG AAAAGGAGAG CTACTTGCAG 
TATGAACTTC GTCGCTACCG CACTTATGTG AGCATTTTGA GCGCCAAGAT GCTGGCTGAT 
CAGCAGCAGC TGGAGCTGCA GGCGCAGCAG CCCTCTCCAG CGTCGCACGA GGAAGAGGCC 
GACACATTCC CAGTGGGCAC CACCGCCTGC ACGCCACCCA CGCCGCAAAG TATTAACCAG 
AAGGATCAGC AGAAGGAGCA GCAGCAGCAA CAGCCAACGA ACAGTCCGCC CAGAATCTCG 
GTGTCAACCA ATTTGGCCTG GCGCAAGATC TTCTGGGACT CAGTTTGCTG CGGTGTTCTG 
TTGTGTTGCT GGCATTATTG TACTGTGCTC ACTGTTATTC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788718.3
CDS	527..5191
Translation	MSEELKVVLRRSEQHSGFGFSLLGTTGPPHVIYDIVENSPAADCGAVEAGDVILKVNGTDVHRYTTKEVLKCLRLSEQLVTLELKRDPKLKARIKEQLANTQSPHYVDIESPNIYDYHSSSTNSSPNHRPNAGGKGAATTPSQTGLRYKSPTHLPSLRQNSSPLLASGSTTTTTTATHTHSHSRNSSASSTKIKVVETSITTSTTNVVGLTSPTGSVGGGVGGEATSPTFRPSRIPQALTKCAVPKPVPVLHSPQNKRPRPSQIPTKAANGNGNGHTAHLPPQSLQHSNSYSGSPVTRQRFADREPEREPEPNSAPPQPAKAPRFEAYMMTGDLILNLSRTPQTSNPLPAQAKKIDSLRDSPSRLVNPRINGALAPRASGESSPTSSSSVDSPTNTSSDSVKREAKLLQKQQQQQQQTYQQQQQRDSINNSYNRKDSLTNDTLLMCEELEPDEEGEYVLEEDNKQQRQRQQQQRYRQQQNQQRYEYYQNEDELEEQEEVEEEREEDQTHYDITNIETYQSGVGRGDDDDSDRQCLVDDDDDDDAYDDEENDAGDEDYSTNSLGSGSAKQRLRALKQRTATRQQQRNRDAVDCAGRSGSGSSSTTVKSEAGGLGLDETSFSVPTSPISLSTPLIDKETANSVPTSPEPSSLVPESSSGAGAGAVVVRRHNGHVVRKCDAAGFRTSKSEDHLQQIQREGIAAVIPIDIDEDVNSSLNTLLDTRQDSEDSQASDRDRIVWTYNAPLQPHQLAALQRQQQQQEQQFQQQQQQLHQQHLQQQQQLQQQHQQQQQQQQQQQQQQLYGQQSHSNSHSSSISSSPQHSAVGSPASPTSVSSSVMSSSGSKGALGLGSSSNGPMAAVQQQQLREREQGGQVAQPPSGIPGLLSCPGGGCGNNGGGGGIGGGGNNDQSVSEAISNISSPDYQDDDNLLSSRDILGGMVLSDPSDSDSTILVSDAAAHQRQQLKQQLRAQQQQQRERERDRDRDREQSEHKVVIQVRGLDSNSSGGNGTNGRSEEDVVTLTDEPLGTMTVGMRDASPPVSDDGSDVESLHSYHYSPKAVDMPSAIRLAKRLHSLDGFKKSDVSRHLSKNNDFSRAVADEYLKHFTFEKKSLDQALREFLQQFSLSGETQERERVLVHFSKRFLDCNPGTFNSQDAVHTLTCAIMLLNTDLHGQNINRKMSCAEFVDNLADLNDGENFPKDVLKSLYQAIKTKPLEWALDEEAGDLQQQRANNSALGNVGLNPFLDPPELATAVEYKKGYVMRKCCYDSSFKKTPFGKRSWKMFYCTLRDLVLYLHKDEHGFRKSQMSDNLHNAIRIHHALATKANDYTKKQHVFRLQTADQAEYLFQTSDSKELQSWVETINYVCAAISAPPLEGGVGSQKRFQRPLLPSKQSKLMLKEQLDSHEVQLAQLDQELNEHKKGPIPSKGLALQNYKEKESYLQYELRRYRTYVSILSAKMLADQQQLELQAQQPSPASHEEEADTFPVGTTACTPPTPQSINQKDQQKEQQQQQPTNSPPRISVSTNLAWRKIFWDSVCCGVLLCCWHYCTVLTVIP

Target ORF information:

RefSeq Version	NM_176541.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster exchange factor for Arf 6, transcript variant I (Efa6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176541.3

ORF Insert Sequence:

ATGAGCGAGG AACTGAAAGT GGTGCTGCGG CGCAGCGAGC AGCATTCCGG TTTCGGGTTT 
TCGCTACTCG GAACCACCGG ACCGCCACAT GTCATCTACG ACATCGTCGA GAATTCGCCG 
GCAGCCGATT GCGGAGCGGT TGAGGCCGGG GATGTCATCC TCAAAGTCAA CGGAACGGAT 
GTCCATCGCT ATACAACGAA GGAAGTTCTC AAATGCCTGC GCCTGTCGGA ACAGCTGGTG 
ACCTTGGAGC TGAAGCGAGA TCCCAAGCTC AAGGCGCGTA TCAAAGAGCA GCTGGCCAAC 
ACCCAGAGTC CGCACTATGT GGACATTGAG TCGCCGAACA TCTACGATTA CCACAGCAGC 
AGCACCAACT CCTCGCCGAA TCACCGGCCG AACGCTGGTG GCAAGGGGGC GGCGACCACG 
CCCTCGCAGA CCGGACTGCG CTACAAGTCA CCGACGCACT TGCCCTCGCT GCGCCAGAAT 
TCGTCGCCAC TGCTGGCGAG TGGATCCACC ACGACCACAA CCACCGCCAC GCATACGCAC 
AGCCACAGTC GAAACTCCTC GGCCAGCTCC ACCAAGATTA AGGTGGTGGA GACAAGCATC 
ACCACCTCGA CCACGAACGT AGTGGGCCTC ACATCGCCCA CCGGTAGTGT TGGGGGTGGT 
GTTGGTGGGG AGGCCACTTC GCCCACCTTC CGCCCCTCAC GCATTCCACA GGCGCTCACC 
AAATGTGCCG TACCCAAGCC CGTGCCCGTT CTCCATTCGC CGCAGAATAA GCGGCCACGC 
CCATCGCAGA TTCCGACAAA GGCGGCGAAC GGAAACGGGA ACGGACATAC CGCCCATCTG 
CCACCGCAAT CGTTGCAGCA TTCCAACAGC TACAGCGGCA GTCCGGTGAC TCGGCAGCGG 
TTTGCCGATA GGGAGCCGGA GCGGGAACCG GAACCGAATT CGGCGCCACC GCAGCCGGCG 
AAGGCGCCAC GCTTTGAGGC TTACATGATG ACCGGTGACC TTATTCTCAA TCTGTCCCGG 
ACACCGCAGA CCAGCAATCC GCTTCCCGCC CAGGCCAAAA AGATTGACAG TCTTCGCGAT 
TCACCCAGTC GTTTGGTAAA TCCGCGTATC AACGGCGCAC TAGCACCGCG TGCCTCTGGT 
GAATCCTCGC CCACGTCCTC CTCTTCGGTA GACTCGCCCA CCAACACCAG CTCAGATTCC 
GTGAAGCGCG AGGCAAAGCT GCTGCAGAAA CAACAGCAGC AGCAGCAACA AACCTACCAA 
CAACAACAGC AGCGGGACAG CATCAACAAC AGCTACAATC GTAAGGATTC GCTGACCAAC 
GATACGCTGC TCATGTGCGA GGAGTTGGAG CCGGACGAGG AGGGCGAGTA TGTCCTGGAA 
GAGGACAACA AACAGCAGCG GCAGCGCCAA CAGCAACAAC GATACCGCCA GCAGCAAAAT 
CAGCAACGCT ACGAATACTA CCAAAACGAG GACGAGCTGG AGGAGCAGGA GGAGGTTGAG 
GAGGAGCGCG AGGAGGATCA AACACACTAC GACATCACCA ATATTGAAAC CTACCAGAGC 
GGTGTGGGCC GGGGTGACGA CGATGACAGT GATCGGCAGT GTCTGGTAGA CGACGACGAT 
GACGATGATG CCTACGATGA TGAGGAGAAC GATGCGGGCG ACGAGGACTA CTCCACCAAC 
TCGCTGGGCT CCGGTTCAGC CAAGCAACGA TTGCGGGCGT TGAAACAGCG CACTGCCACC 
CGCCAGCAGC AGCGAAATCG GGATGCCGTC GACTGTGCAG GACGCTCCGG ATCGGGATCA 
TCATCTACCA CGGTCAAGAG CGAGGCTGGC GGCCTGGGCC TAGATGAAAC CTCCTTCTCA 
GTGCCAACCT CTCCGATCTC GCTGTCGACG CCGCTGATCG ACAAGGAGAC GGCCAACTCG 
GTGCCCACGA GCCCGGAGCC CAGTTCGCTT GTTCCGGAGT CGAGCAGTGG CGCTGGCGCC 
GGAGCCGTGG TGGTGCGCCG GCATAACGGA CATGTGGTGC GGAAGTGTGA TGCCGCCGGT 
TTCCGCACCA GCAAGTCTGA GGACCATTTG CAGCAGATCC AGCGCGAGGG CATCGCCGCT 
GTGATACCCA TCGACATTGA TGAGGATGTG AATAGTTCGC TGAACACGCT GCTGGACACG 
CGTCAGGACT CCGAGGACTC GCAGGCATCG GATCGCGATC GGATCGTGTG GACTTATAAT 
GCGCCACTCC AGCCGCATCA GTTGGCGGCT CTTCAGCGAC AGCAGCAACA GCAAGAGCAG 
CAATTCCAAC AGCAGCAGCA GCAGCTTCAC CAGCAACATC TGCAGCAACA GCAGCAGCTT 
CAGCAGCAAC ATCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAGCA GCAACTCTAC 
GGTCAGCAAT CGCATTCAAA TTCACATTCA AGTTCCATTA GTTCGTCGCC CCAGCACTCG 
GCTGTGGGCA GTCCGGCCTC GCCCACGTCG GTTTCCTCGT CGGTGATGTC CTCGTCGGGC 
TCCAAGGGCG CCCTGGGCCT GGGCAGCAGC AGCAATGGTC CCATGGCCGC CGTGCAGCAA 
CAACAGCTGA GAGAGAGGGA GCAGGGCGGC CAGGTCGCGC AACCGCCCAG CGGGATTCCT 
GGCTTGCTTA GCTGCCCAGG TGGTGGGTGC GGAAACAATG GTGGTGGTGG AGGCATTGGT 
GGTGGTGGCA ACAACGATCA GAGCGTCTCA GAGGCCATTT CGAATATATC TAGTCCCGAC 
TACCAAGACG ACGATAATTT ATTGAGTTCT CGCGATATTC TAGGCGGCAT GGTACTTAGC 
GATCCCAGTG ATTCGGACTC CACCATCCTC GTCTCGGACG CGGCCGCCCA TCAGCGCCAG 
CAGCTCAAGC AGCAGTTGCG CGCCCAGCAG CAACAGCAGA GAGAAAGGGA ACGGGATAGG 
GATCGAGACA GGGAACAGTC CGAGCACAAA GTGGTCATAC AAGTGCGCGG TCTGGACAGT 
AACAGCAGCG GCGGCAACGG AACTAACGGC CGCTCCGAGG AGGATGTGGT CACGCTGACG 
GACGAGCCGC TGGGCACGAT GACCGTTGGC ATGCGGGACG CCTCGCCGCC AGTCTCTGAC 
GATGGCAGCG ATGTGGAGTC CCTGCACTCG TATCACTACT CGCCCAAGGC CGTGGACATG 
CCCTCGGCCA TACGCCTGGC CAAAAGACTG CACTCCCTCG ACGGTTTCAA GAAGAGCGAT 
GTGTCGCGAC ACCTCAGCAA GAACAATGAC TTTAGTCGAG CGGTGGCCGA TGAGTATCTC 
AAACATTTTA CCTTTGAGAA GAAGTCACTT GACCAAGCGC TGCGTGAGTT CTTGCAGCAA 
TTCTCGCTGT CCGGCGAAAC GCAGGAACGG GAACGAGTGC TGGTGCACTT TTCCAAGCGC 
TTCCTCGACT GCAATCCTGG CACCTTTAAC TCGCAGGACG CCGTGCACAC GCTGACCTGT 
GCCATAATGT TGCTGAATAC GGACTTGCAC GGCCAGAACA TAAATCGTAA GATGAGCTGT 
GCGGAATTCG TCGACAACCT GGCAGATCTC AACGATGGCG AGAACTTTCC CAAGGATGTG 
CTCAAGTCGC TTTACCAGGC CATTAAGACC AAGCCGCTTG AGTGGGCACT AGATGAAGAG 
GCAGGTGATC TGCAGCAGCA AAGAGCCAAC AATAGTGCCC TGGGCAATGT GGGTCTCAAT 
CCCTTCCTCG ATCCCCCAGA ACTGGCCACA GCTGTGGAAT ACAAAAAAGG CTATGTGATG 
CGTAAATGCT GCTATGACAG CAGCTTTAAG AAGACTCCCT TTGGCAAACG CTCCTGGAAG 
ATGTTCTACT GCACGCTGCG TGATCTTGTG CTGTATTTGC ATAAGGATGA GCACGGTTTT 
CGTAAAAGTC AAATGTCCGA CAATCTGCAC AATGCAATAC GCATACATCA CGCACTGGCC 
ACCAAGGCCA ACGACTACAC CAAGAAGCAA CATGTGTTCC GGCTGCAGAC GGCCGACCAG 
GCCGAGTATC TGTTCCAGAC CAGCGACTCC AAGGAGCTGC AGTCGTGGGT GGAGACGATA 
AATTACGTGT GCGCCGCTAT ATCAGCGCCT CCGCTCGAGG GAGGGGTGGG CAGTCAGAAA 
CGATTCCAAC GTCCGCTCCT GCCCAGCAAA CAATCCAAGC TGATGCTGAA AGAGCAGTTG 
GACTCGCACG AGGTGCAGCT GGCGCAGTTG GATCAGGAGC TTAACGAGCA CAAGAAGGGT 
CCTATTCCCA GCAAGGGCTT GGCTCTGCAG AACTACAAGG AAAAGGAGAG CTACTTGCAG 
TATGAACTTC GTCGCTACCG CACTTATGTG AGCATTTTGA GCGCCAAGAT GCTGGCTGAT 
CAGCAGCAGC TGGAGCTGCA GGCGCAGCAG CCCTCTCCAG CGTCGCACGA GGAAGAGGCC 
GACACATTCC CAGTGGGCAC CACCGCCTGC ACGCCACCCA CGCCGCAAAG TATTAACCAG 
AAGGATCAGC AGAAGGAGCA GCAGCAGCAA CAGCCAACGA ACAGTCCGCC CAGAATCTCG 
GTGTCAACCA ATTTGGCCTG GCGCAAGATC TTCTGGGACT CAGTTTGCTG CGGTGTTCTG 
TTGTGTTGCT GGCATTATTG TACTGTGCTC ACTGTTATTC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Efa6 ( NM_001104404.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097874.1 Drosophila melanogaster exchange factor for Arf 6, transcript variant D (Efa6), mRNA.

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CloneID	OFa08662
Clone ID Related Accession (Same CDS sequence)	NM_001104404.2
Accession Version	NM_001104404.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	exchange factor for Arf 6, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_001104404.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGAGG AACTGAAAGT GGTGCTGCGG CGCAGCGAGC AGCATTCCGG TTTCGGGTTT 
TCGCTACTCG GAACCACCGG ACCGCCACAT GTCATCTACG ACATCGTCGA GAATTCGCCG 
GCAGCCGATT GCGGAGCGGT TGAGGCCGGG GATGTCATCC TCAAAGTCAA CGGAACGGAT 
GTCCATCGCT ATACAACGAA GGAAGTTCTC AAATGCCTGC GCCTGTCGGA ACAGCTGGTG 
ACCTTGGAGC TGAAGCGAGA TCCCAAGCTC AAGGCGCGTA TCAAAGAGCA GCTGGCCAAC 
ACCCAGAGTC CGCACTATGT GGACATTGAG TCGCCGAACA TCTACGATTA CCACAGCAGC 
AGCACCAACT CCTCGCCGAA TCACCGGCCG AACGCTGGTG GCAAGGGGGC GGCGACCACG 
CCCTCGCAGA CCGGACTGCG CTACAAGTCA CCGACGCACT TGCCCTCGCT GCGCCAGAAT 
TCGTCGCCAC TGCTGGCGAG TGGATCCACC ACGACCACAA CCACCGCCAC GCATACGCAC 
AGCCACAGTC GAAACTCCTC GGCCAGCTCC ACCAAGATTA AGGTGGTGGA GACAAGCATC 
ACCACCTCGA CCACGAACGT AGTGGGCCTC ACATCGCCCA CCGGTAGTGT TGGGGGTGGT 
GTTGGTGGGG AGGCCACTTC GCCCACCTTC CGCCCCTCAC GCATTCCACA GGCGCTCACC 
AAATGTGCCG TACCCAAGCC CGTGCCCGTT CTCCATTCGC CGCAGAATAA GCGGCCACGC 
CCATCGCAGA TTCCGACAAA GGCGGCGAAC GGAAACGGGA ACGGACATAC CGCCCATCTG 
CCACCGCAAT CGTTGCAGCA TTCCAACAGC TACAGCGGCA GTCCGGTGAC TCGGCAGCGG 
TTTGCCGATA GGGAGCCGGA GCGGGAACCG GAACCGAATT CGGCGCCACC GCAGCCGGCG 
AAGGCGCCAC GCTTTGAGGC TTACATGATG ACCGGTGACC TTATTCTCAA TCTGTCCCGG 
ACACCGCAGA CCAGCAATCC GCTTCCCGCC CAGGCCAAAA AGATTGACAG TCTTCGCGAT 
TCACCCAGTC GTTTGGTAAA TCCGCGTATC AACGGCGCAC TAGCACCGCG TGCCTCTGGT 
GAATCCTCGC CCACGTCCTC CTCTTCGGTA GACTCGCCCA CCAACACCAG CTCAGATTCC 
GTGAAGCGCG AGGCAAAGCT GCTGCAGAAA CAACAGCAGC AGCAGCAACA AACCTACCAA 
CAACAACAGC AGCGGGACAG CATCAACAAC AGCTACAATC GTAAGGATTC GCTGACCAAC 
GATACGCTGC TCATGTGCGA GGAGTTGGAG CCGGACGAGG AGGGCGAGTA TGTCCTGGAA 
GAGGACAACA AACAGCAGCG GCAGCGCCAA CAGCAACAAC GATACCGCCA GCAGCAAAAT 
CAGCAACGCT ACGAATACTA CCAAAACGAG GACGAGCTGG AGGAGCAGGA GGAGGTTGAG 
GAGGAGCGCG AGGAGGATCA AACACACTAC GACATCACCA ATATTGAAAC CTACCAGAGC 
GGTGTGGGCC GGGGTGACGA CGATGACAGT GATCGGCAGT GTCTGGTAGA CGACGACGAT 
GACGATGATG CCTACGATGA TGAGGAGAAC GATGCGGGCG ACGAGGACTA CTCCACCAAC 
TCGCTGGGCT CCGGTTCAGC CAAGCAACGA TTGCGGGCGT TGAAACAGCG CACTGCCACC 
CGCCAGCAGC AGCGAAATCG GGATGCCGTC GACTGTGCAG GACGCTCCGG ATCGGGATCA 
TCATCTACCA CGGTCAAGAG CGAGGCTGGC GGCCTGGGCC TAGATGAAAC CTCCTTCTCA 
GTGCCAACCT CTCCGATCTC GCTGTCGACG CCGCTGATCG ACAAGGAGAC GGCCAACTCG 
GTGCCCACGA GCCCGGAGCC CAGTTCGCTT GTTCCGGAGT CGAGCAGTGG CGCTGGCGCC 
GGAGCCGTGG TGGTGCGCCG GCATAACGGA CATGTGGTGC GGAAGTGTGA TGCCGCCGGT 
TTCCGCACCA GCAAGTCTGA GGACCATTTG CAGCAGATCC AGCGCGAGGG CATCGCCGCT 
GTGATACCCA TCGACATTGA TGAGGATGTG AATAGTTCGC TGAACACGCT GCTGGACACG 
CGTCAGGACT CCGAGGACTC GCAGGCATCG GATCGCGATC GGATCGTGTG GACTTATAAT 
GCGCCACTCC AGCCGCATCA GTTGGCGGCT CTTCAGCGAC AGCAGCAACA GCAAGAGCAG 
CAATTCCAAC AGCAGCAGCA GCAGCTTCAC CAGCAACATC TGCAGCAACA GCAGCAGCTT 
CAGCAGCAAC ATCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAGCA GCAACTCTAC 
GGTCAGCAAT CGCATTCAAA TTCACATTCA AGTTCCATTA GTTCGTCGCC CCAGCACTCG 
GCTGTGGGCA GTCCGGCCTC GCCCACGTCG GTTTCCTCGT CGGTGATGTC CTCGTCGGGC 
TCCAAGGGCG CCCTGGGCCT GGGCAGCAGC AGCAATGGTC CCATGGCCGC CGTGCAGCAA 
CAACAGCTGA GAGAGAGGGA GCAGGGCGGC CAGGTCGCGC AACCGCCCAG CGGGATTCCT 
GGCTTGCTTA GCTGCCCAGG TGGTGGGTGC GGAAACAATG GTGGTGGTGG AGGCATTGGT 
GGTGGTGGCA ACAACGATCA GAGCGTCTCA GAGGCCATTT CGAATATATC TAGTCCCGAC 
TACCAAGACG ACGATAATTT ATTGAGTTCT CGCGATATTC TAGGCGGCAT GGTACTTAGC 
GATCCCAGTG ATTCGGACTC CACCATCCTC GTCTCGGACG CGGCCGCCCA TCAGCGCCAG 
CAGCTCAAGC AGCAGTTGCG CGCCCAGCAG CAACAGCAGA GAGAAAGGGA ACGGGATAGG 
GATCGAGACA GGGAACAGTC CGAGCACAAA GTGGTCATAC AAGTGCGCGG TCTGGACAGT 
AACAGCAGCG GCGGCAACGG AACTAACGGC CGCTCCGAGG AGGATGTGGT CACGCTGACG 
GACGAGCCGC TGGGCACGAT GACCGTTGGC ATGCGGGACG CCTCGCCGCC AGTCTCTGAC 
GATGGCAGCG ATGTGGAGTC CCTGCACTCG TATCACTACT CGCCCAAGGC CGTGGACATG 
CCCTCGGCCA TACGCCTGGC CAAAAGACTG CACTCCCTCG ACGGTTTCAA GAAGAGCGAT 
GTGTCGCGAC ACCTCAGCAA GAACAATGAC TTTAGTCGAG CGGTGGCCGA TGAGTATCTC 
AAACATTTTA CCTTTGAGAA GAAGTCACTT GACCAAGCGC TGCGTGAGTT CTTGCAGCAA 
TTCTCGCTGT CCGGCGAAAC GCAGGAACGG GAACGAGTGC TGGTGCACTT TTCCAAGCGC 
TTCCTCGACT GCAATCCTGG CACCTTTAAC TCGCAGGACG CCGTGCACAC GCTGACCTGT 
GCCATAATGT TGCTGAATAC GGACTTGCAC GGCCAGAACA TAAATCGTAA GATGAGCTGT 
GCGGAATTCG TCGACAACCT GGCAGATCTC AACGATGGCG AGAACTTTCC CAAGGATGTG 
CTCAAGTCGC TTTACCAGGC CATTAAGACC AAGCCGCTTG AGTGGGCACT AGATGAAGAG 
GCAGGTGATC TGCAGCAGCA AAGAGCCAAC AATAGTGCCC TGGGCAATGT GGGTCTCAAT 
CCCTTCCTCG ATCCCCCAGA ACTGGCCACA GCTGTGGAAT ACAAAAAAGG CTATGTGATG 
CGTAAATGCT GCTATGACAG CAGCTTTAAG AAGACTCCCT TTGGCAAACG CTCCTGGAAG 
ATGTTCTACT GCACGCTGCG TGATCTTGTG CTGTATTTGC ATAAGGATGA GCACGGTTTT 
CGTAAAAGTC AAATGTCCGA CAATCTGCAC AATGCAATAC GCATACATCA CGCACTGGCC 
ACCAAGGCCA ACGACTACAC CAAGAAGCAA CATGTGTTCC GGCTGCAGAC GGCCGACCAG 
GCCGAGTATC TGTTCCAGAC CAGCGACTCC AAGGAGCTGC AGTCGTGGGT GGAGACGATA 
AATTACGTGT GCGCCGCTAT ATCAGCGCCT CCGCTCGAGG GAGGGGTGGG CAGTCAGAAA 
CGATTCCAAC GTCCGCTCCT GCCCAGCAAA CAATCCAAGC TGATGCTGAA AGAGCAGTTG 
GACTCGCACG AGGTGCAGCT GGCGCAGTTG GATCAGGAGC TTAACGAGCA CAAGAAGGGT 
CCTATTCCCA GCAAGGGCTT GGCTCTGCAG AACTACAAGG AAAAGGAGAG CTACTTGCAG 
TATGAACTTC GTCGCTACCG CACTTATGTG AGCATTTTGA GCGCCAAGAT GCTGGCTGAT 
CAGCAGCAGC TGGAGCTGCA GGCGCAGCAG CCCTCTCCAG CGTCGCACGA GGAAGAGGCC 
GACACATTCC CAGTGGGCAC CACCGCCTGC ACGCCACCCA CGCCGCAAAG TATTAACCAG 
AAGGATCAGC AGAAGGAGCA GCAGCAGCAA CAGCCAACGA ACAGAAAAGA GAAGAAAAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097874.1
CDS	527..5092
Translation	MSEELKVVLRRSEQHSGFGFSLLGTTGPPHVIYDIVENSPAADCGAVEAGDVILKVNGTDVHRYTTKEVLKCLRLSEQLVTLELKRDPKLKARIKEQLANTQSPHYVDIESPNIYDYHSSSTNSSPNHRPNAGGKGAATTPSQTGLRYKSPTHLPSLRQNSSPLLASGSTTTTTTATHTHSHSRNSSASSTKIKVVETSITTSTTNVVGLTSPTGSVGGGVGGEATSPTFRPSRIPQALTKCAVPKPVPVLHSPQNKRPRPSQIPTKAANGNGNGHTAHLPPQSLQHSNSYSGSPVTRQRFADREPEREPEPNSAPPQPAKAPRFEAYMMTGDLILNLSRTPQTSNPLPAQAKKIDSLRDSPSRLVNPRINGALAPRASGESSPTSSSSVDSPTNTSSDSVKREAKLLQKQQQQQQQTYQQQQQRDSINNSYNRKDSLTNDTLLMCEELEPDEEGEYVLEEDNKQQRQRQQQQRYRQQQNQQRYEYYQNEDELEEQEEVEEEREEDQTHYDITNIETYQSGVGRGDDDDSDRQCLVDDDDDDDAYDDEENDAGDEDYSTNSLGSGSAKQRLRALKQRTATRQQQRNRDAVDCAGRSGSGSSSTTVKSEAGGLGLDETSFSVPTSPISLSTPLIDKETANSVPTSPEPSSLVPESSSGAGAGAVVVRRHNGHVVRKCDAAGFRTSKSEDHLQQIQREGIAAVIPIDIDEDVNSSLNTLLDTRQDSEDSQASDRDRIVWTYNAPLQPHQLAALQRQQQQQEQQFQQQQQQLHQQHLQQQQQLQQQHQQQQQQQQQQQQQQLYGQQSHSNSHSSSISSSPQHSAVGSPASPTSVSSSVMSSSGSKGALGLGSSSNGPMAAVQQQQLREREQGGQVAQPPSGIPGLLSCPGGGCGNNGGGGGIGGGGNNDQSVSEAISNISSPDYQDDDNLLSSRDILGGMVLSDPSDSDSTILVSDAAAHQRQQLKQQLRAQQQQQRERERDRDRDREQSEHKVVIQVRGLDSNSSGGNGTNGRSEEDVVTLTDEPLGTMTVGMRDASPPVSDDGSDVESLHSYHYSPKAVDMPSAIRLAKRLHSLDGFKKSDVSRHLSKNNDFSRAVADEYLKHFTFEKKSLDQALREFLQQFSLSGETQERERVLVHFSKRFLDCNPGTFNSQDAVHTLTCAIMLLNTDLHGQNINRKMSCAEFVDNLADLNDGENFPKDVLKSLYQAIKTKPLEWALDEEAGDLQQQRANNSALGNVGLNPFLDPPELATAVEYKKGYVMRKCCYDSSFKKTPFGKRSWKMFYCTLRDLVLYLHKDEHGFRKSQMSDNLHNAIRIHHALATKANDYTKKQHVFRLQTADQAEYLFQTSDSKELQSWVETINYVCAAISAPPLEGGVGSQKRFQRPLLPSKQSKLMLKEQLDSHEVQLAQLDQELNEHKKGPIPSKGLALQNYKEKESYLQYELRRYRTYVSILSAKMLADQQQLELQAQQPSPASHEEEADTFPVGTTACTPPTPQSINQKDQQKEQQQQQPTNRKEKKKK

Target ORF information:

RefSeq Version	NM_001104404.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster exchange factor for Arf 6, transcript variant D (Efa6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104404.2

ORF Insert Sequence:

ATGAGCGAGG AACTGAAAGT GGTGCTGCGG CGCAGCGAGC AGCATTCCGG TTTCGGGTTT 
TCGCTACTCG GAACCACCGG ACCGCCACAT GTCATCTACG ACATCGTCGA GAATTCGCCG 
GCAGCCGATT GCGGAGCGGT TGAGGCCGGG GATGTCATCC TCAAAGTCAA CGGAACGGAT 
GTCCATCGCT ATACAACGAA GGAAGTTCTC AAATGCCTGC GCCTGTCGGA ACAGCTGGTG 
ACCTTGGAGC TGAAGCGAGA TCCCAAGCTC AAGGCGCGTA TCAAAGAGCA GCTGGCCAAC 
ACCCAGAGTC CGCACTATGT GGACATTGAG TCGCCGAACA TCTACGATTA CCACAGCAGC 
AGCACCAACT CCTCGCCGAA TCACCGGCCG AACGCTGGTG GCAAGGGGGC GGCGACCACG 
CCCTCGCAGA CCGGACTGCG CTACAAGTCA CCGACGCACT TGCCCTCGCT GCGCCAGAAT 
TCGTCGCCAC TGCTGGCGAG TGGATCCACC ACGACCACAA CCACCGCCAC GCATACGCAC 
AGCCACAGTC GAAACTCCTC GGCCAGCTCC ACCAAGATTA AGGTGGTGGA GACAAGCATC 
ACCACCTCGA CCACGAACGT AGTGGGCCTC ACATCGCCCA CCGGTAGTGT TGGGGGTGGT 
GTTGGTGGGG AGGCCACTTC GCCCACCTTC CGCCCCTCAC GCATTCCACA GGCGCTCACC 
AAATGTGCCG TACCCAAGCC CGTGCCCGTT CTCCATTCGC CGCAGAATAA GCGGCCACGC 
CCATCGCAGA TTCCGACAAA GGCGGCGAAC GGAAACGGGA ACGGACATAC CGCCCATCTG 
CCACCGCAAT CGTTGCAGCA TTCCAACAGC TACAGCGGCA GTCCGGTGAC TCGGCAGCGG 
TTTGCCGATA GGGAGCCGGA GCGGGAACCG GAACCGAATT CGGCGCCACC GCAGCCGGCG 
AAGGCGCCAC GCTTTGAGGC TTACATGATG ACCGGTGACC TTATTCTCAA TCTGTCCCGG 
ACACCGCAGA CCAGCAATCC GCTTCCCGCC CAGGCCAAAA AGATTGACAG TCTTCGCGAT 
TCACCCAGTC GTTTGGTAAA TCCGCGTATC AACGGCGCAC TAGCACCGCG TGCCTCTGGT 
GAATCCTCGC CCACGTCCTC CTCTTCGGTA GACTCGCCCA CCAACACCAG CTCAGATTCC 
GTGAAGCGCG AGGCAAAGCT GCTGCAGAAA CAACAGCAGC AGCAGCAACA AACCTACCAA 
CAACAACAGC AGCGGGACAG CATCAACAAC AGCTACAATC GTAAGGATTC GCTGACCAAC 
GATACGCTGC TCATGTGCGA GGAGTTGGAG CCGGACGAGG AGGGCGAGTA TGTCCTGGAA 
GAGGACAACA AACAGCAGCG GCAGCGCCAA CAGCAACAAC GATACCGCCA GCAGCAAAAT 
CAGCAACGCT ACGAATACTA CCAAAACGAG GACGAGCTGG AGGAGCAGGA GGAGGTTGAG 
GAGGAGCGCG AGGAGGATCA AACACACTAC GACATCACCA ATATTGAAAC CTACCAGAGC 
GGTGTGGGCC GGGGTGACGA CGATGACAGT GATCGGCAGT GTCTGGTAGA CGACGACGAT 
GACGATGATG CCTACGATGA TGAGGAGAAC GATGCGGGCG ACGAGGACTA CTCCACCAAC 
TCGCTGGGCT CCGGTTCAGC CAAGCAACGA TTGCGGGCGT TGAAACAGCG CACTGCCACC 
CGCCAGCAGC AGCGAAATCG GGATGCCGTC GACTGTGCAG GACGCTCCGG ATCGGGATCA 
TCATCTACCA CGGTCAAGAG CGAGGCTGGC GGCCTGGGCC TAGATGAAAC CTCCTTCTCA 
GTGCCAACCT CTCCGATCTC GCTGTCGACG CCGCTGATCG ACAAGGAGAC GGCCAACTCG 
GTGCCCACGA GCCCGGAGCC CAGTTCGCTT GTTCCGGAGT CGAGCAGTGG CGCTGGCGCC 
GGAGCCGTGG TGGTGCGCCG GCATAACGGA CATGTGGTGC GGAAGTGTGA TGCCGCCGGT 
TTCCGCACCA GCAAGTCTGA GGACCATTTG CAGCAGATCC AGCGCGAGGG CATCGCCGCT 
GTGATACCCA TCGACATTGA TGAGGATGTG AATAGTTCGC TGAACACGCT GCTGGACACG 
CGTCAGGACT CCGAGGACTC GCAGGCATCG GATCGCGATC GGATCGTGTG GACTTATAAT 
GCGCCACTCC AGCCGCATCA GTTGGCGGCT CTTCAGCGAC AGCAGCAACA GCAAGAGCAG 
CAATTCCAAC AGCAGCAGCA GCAGCTTCAC CAGCAACATC TGCAGCAACA GCAGCAGCTT 
CAGCAGCAAC ATCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAGCA GCAACTCTAC 
GGTCAGCAAT CGCATTCAAA TTCACATTCA AGTTCCATTA GTTCGTCGCC CCAGCACTCG 
GCTGTGGGCA GTCCGGCCTC GCCCACGTCG GTTTCCTCGT CGGTGATGTC CTCGTCGGGC 
TCCAAGGGCG CCCTGGGCCT GGGCAGCAGC AGCAATGGTC CCATGGCCGC CGTGCAGCAA 
CAACAGCTGA GAGAGAGGGA GCAGGGCGGC CAGGTCGCGC AACCGCCCAG CGGGATTCCT 
GGCTTGCTTA GCTGCCCAGG TGGTGGGTGC GGAAACAATG GTGGTGGTGG AGGCATTGGT 
GGTGGTGGCA ACAACGATCA GAGCGTCTCA GAGGCCATTT CGAATATATC TAGTCCCGAC 
TACCAAGACG ACGATAATTT ATTGAGTTCT CGCGATATTC TAGGCGGCAT GGTACTTAGC 
GATCCCAGTG ATTCGGACTC CACCATCCTC GTCTCGGACG CGGCCGCCCA TCAGCGCCAG 
CAGCTCAAGC AGCAGTTGCG CGCCCAGCAG CAACAGCAGA GAGAAAGGGA ACGGGATAGG 
GATCGAGACA GGGAACAGTC CGAGCACAAA GTGGTCATAC AAGTGCGCGG TCTGGACAGT 
AACAGCAGCG GCGGCAACGG AACTAACGGC CGCTCCGAGG AGGATGTGGT CACGCTGACG 
GACGAGCCGC TGGGCACGAT GACCGTTGGC ATGCGGGACG CCTCGCCGCC AGTCTCTGAC 
GATGGCAGCG ATGTGGAGTC CCTGCACTCG TATCACTACT CGCCCAAGGC CGTGGACATG 
CCCTCGGCCA TACGCCTGGC CAAAAGACTG CACTCCCTCG ACGGTTTCAA GAAGAGCGAT 
GTGTCGCGAC ACCTCAGCAA GAACAATGAC TTTAGTCGAG CGGTGGCCGA TGAGTATCTC 
AAACATTTTA CCTTTGAGAA GAAGTCACTT GACCAAGCGC TGCGTGAGTT CTTGCAGCAA 
TTCTCGCTGT CCGGCGAAAC GCAGGAACGG GAACGAGTGC TGGTGCACTT TTCCAAGCGC 
TTCCTCGACT GCAATCCTGG CACCTTTAAC TCGCAGGACG CCGTGCACAC GCTGACCTGT 
GCCATAATGT TGCTGAATAC GGACTTGCAC GGCCAGAACA TAAATCGTAA GATGAGCTGT 
GCGGAATTCG TCGACAACCT GGCAGATCTC AACGATGGCG AGAACTTTCC CAAGGATGTG 
CTCAAGTCGC TTTACCAGGC CATTAAGACC AAGCCGCTTG AGTGGGCACT AGATGAAGAG 
GCAGGTGATC TGCAGCAGCA AAGAGCCAAC AATAGTGCCC TGGGCAATGT GGGTCTCAAT 
CCCTTCCTCG ATCCCCCAGA ACTGGCCACA GCTGTGGAAT ACAAAAAAGG CTATGTGATG 
CGTAAATGCT GCTATGACAG CAGCTTTAAG AAGACTCCCT TTGGCAAACG CTCCTGGAAG 
ATGTTCTACT GCACGCTGCG TGATCTTGTG CTGTATTTGC ATAAGGATGA GCACGGTTTT 
CGTAAAAGTC AAATGTCCGA CAATCTGCAC AATGCAATAC GCATACATCA CGCACTGGCC 
ACCAAGGCCA ACGACTACAC CAAGAAGCAA CATGTGTTCC GGCTGCAGAC GGCCGACCAG 
GCCGAGTATC TGTTCCAGAC CAGCGACTCC AAGGAGCTGC AGTCGTGGGT GGAGACGATA 
AATTACGTGT GCGCCGCTAT ATCAGCGCCT CCGCTCGAGG GAGGGGTGGG CAGTCAGAAA 
CGATTCCAAC GTCCGCTCCT GCCCAGCAAA CAATCCAAGC TGATGCTGAA AGAGCAGTTG 
GACTCGCACG AGGTGCAGCT GGCGCAGTTG GATCAGGAGC TTAACGAGCA CAAGAAGGGT 
CCTATTCCCA GCAAGGGCTT GGCTCTGCAG AACTACAAGG AAAAGGAGAG CTACTTGCAG 
TATGAACTTC GTCGCTACCG CACTTATGTG AGCATTTTGA GCGCCAAGAT GCTGGCTGAT 
CAGCAGCAGC TGGAGCTGCA GGCGCAGCAG CCCTCTCCAG CGTCGCACGA GGAAGAGGCC 
GACACATTCC CAGTGGGCAC CACCGCCTGC ACGCCACCCA CGCCGCAAAG TATTAACCAG 
AAGGATCAGC AGAAGGAGCA GCAGCAGCAA CAGCCAACGA ACAGAAAAGA GAAGAAAAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Efa6 ( NM_001104403.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097873.1 Drosophila melanogaster exchange factor for Arf 6, transcript variant C (Efa6), mRNA.

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CloneID	OFa08663
Clone ID Related Accession (Same CDS sequence)	NM_001104403.2
Accession Version	NM_001104403.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	exchange factor for Arf 6, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_001104403.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGAGG AACTGAAAGT GGTGCTGCGG CGCAGCGAGC AGCATTCCGG TTTCGGGTTT 
TCGCTACTCG GAACCACCGG ACCGCCACAT GTCATCTACG ACATCGTCGA GAATTCGCCG 
GCAGCCGATT GCGGAGCGGT TGAGGCCGGG GATGTCATCC TCAAAGTCAA CGGAACGGAT 
GTCCATCGCT ATACAACGAA GGAAGTTCTC AAATGCCTGC GCCTGTCGGA ACAGCTGGTG 
ACCTTGGAGC TGAAGCGAGA TCCCAAGCTC AAGGCGCGTA TCAAAGAGCA GCTGGCCAAC 
ACCCAGAGTC CGCACTATGT GGACATTGAG TCGCCGAACA TCTACGATTA CCACAGCAGC 
AGCACCAACT CCTCGCCGAA TCACCGGCCG AACGCTGGTG GCAAGGGGGC GGCGACCACG 
CCCTCGCAGA CCGGACTGCG CTACAAGTCA CCGACGCACT TGCCCTCGCT GCGCCAGAAT 
TCGTCGCCAC TGCTGGCGAG TGGATCCACC ACGACCACAA CCACCGCCAC GCATACGCAC 
AGCCACAGTC GAAACTCCTC GGCCAGCTCC ACCAAGATTA AGGTGGTGGA GACAAGCATC 
ACCACCTCGA CCACGAACGT AGTGGGCCTC ACATCGCCCA CCGGTAGTGT TGGGGGTGGT 
GTTGGTGGGG AGGCCACTTC GCCCACCTTC CGCCCCTCAC GCATTCCACA GGCGCTCACC 
AAATGTGCCG TACCCAAGCC CGTGCCCGTT CTCCATTCGC CGCAGAATAA GCGGCCACGC 
CCATCGCAGA TTCCGACAAA GGCGGCGAAC GGAAACGGGA ACGGACATAC CGCCCATCTG 
CCACCGCAAT CGTTGCAGCA TTCCAACAGC TACAGCGGCA GTCCGGTGAC TCGGCAGCGG 
TTTGCCGATA GGGAGCCGGA GCGGGAACCG GAACCGAATT CGGCGCCACC GCAGCCGGCG 
AAGGCGCCAC GCTTTGAGGC TTACATGATG ACCGGTGACC TTATTCTCAA TCTGTCCCGG 
ACACCGCAGA CCAGCAATCC GCTTCCCGCC CAGGCCAAAA AGATTGACAG TCTTCGCGAT 
TCACCCAGTC GTTTGGTAAA TCCGCGTATC AACGGCGCAC TAGCACCGCG TGCCTCTGGT 
GAATCCTCGC CCACGTCCTC CTCTTCGGTA GACTCGCCCA CCAACACCAG CTCAGATTCC 
GTGAAGCGCG AGGCAAAGCT GCTGCAGAAA CAACAGCAGC AGCAGCAACA AACCTACCAA 
CAACAACAGC AGCGGGACAG CATCAACAAC AGCTACAATC GTAAGGATTC GCTGACCAAC 
GATACGCTGC TCATGTGCGA GGAGTTGGAG CCGGACGAGG AGGGCGAGTA TGTCCTGGAA 
GAGGACAACA AACAGCAGCG GCAGCGCCAA CAGCAACAAC GATACCGCCA GCAGCAAAAT 
CAGCAACGCT ACGAATACTA CCAAAACGAG GACGAGCTGG AGGAGCAGGA GGAGGTTGAG 
GAGGAGCGCG AGGAGGATCA AACACACTAC GACATCACCA ATATTGAAAC CTACCAGAGC 
GGTGTGGGCC GGGGTGACGA CGATGACAGT GATCGGCAGT GTCTGGTAGA CGACGACGAT 
GACGATGATG CCTACGATGA TGAGGAGAAC GATGCGGGCG ACGAGGACTA CTCCACCAAC 
TCGCTGGGCT CCGGTTCAGC CAAGCAACGA TTGCGGGCGT TGAAACAGCG CACTGCCACC 
CGCCAGCAGC AGCGAAATCG GGATGCCGTC GACTGTGCAG GACGCTCCGG ATCGGGATCA 
TCATCTACCA CGGTCAAGAG CGAGGCTGGC GGCCTGGGCC TAGATGAAAC CTCCTTCTCA 
GTGCCAACCT CTCCGATCTC GCTGTCGACG CCGCTGATCG ACAAGGAGAC GGCCAACTCG 
GTGCCCACGA GCCCGGAGCC CAGTTCGCTT GTTCCGGAGT CGAGCAGTGG CGCTGGCGCC 
GGAGCCGTGG TGGTGCGCCG GCATAACGGA CATGTGGTGC GGAAGTGTGA TGCCGCCGGT 
TTCCGCACCA GCAAGTCTGA GGACCATTTG CAGCAGATCC AGCGCGAGGG CATCGCCGCT 
GTGATACCCA TCGACATTGA TGAGGATGTG AATAGTTCGC TGAACACGCT GCTGGACACG 
CGTCAGGACT CCGAGGACTC GCAGGCATCG GATCGCGATC GGATCGTGTG GACTTATAAT 
GCGCCACTCC AGCCGCATCA GTTGGCGGCT CTTCAGCGAC AGCAGCAACA GCAAGAGCAG 
CAATTCCAAC AGCAGCAGCA GCAGCTTCAC CAGCAACATC TGCAGCAACA GCAGCAGCTT 
CAGCAGCAAC ATCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAGCA GCAACTCTAC 
GGCGGCATGG TACTTAGCGA TCCCAGTGAT TCGGACTCCA CCATCCTCGT CTCGGACGCG 
GCCGCCCATC AGCGCCAGCA GCTCAAGCAG CAGTTGCGCG CCCAGCAGCA ACAGCAGAGA 
GAAAGGGAAC GGGATAGGGA TCGAGACAGG GAACAGTCCG AGCACAAAGT GGTCATACAA 
GTGCGCGGTC TGGACAGTAA CAGCAGCGGC GGCAACGGAA CTAACGGCCG CTCCGAGGAG 
GATGTGGTCA CGCTGACGGA CGAGCCGCTG GGCACGATGA CCGTTGGCAT GCGGGACGCC 
TCGCCGCCAG TCTCTGACGA TGGCAGCGAT GTGGAGTCCC TGCACTCGTA TCACTACTCG 
CCCAAGGCCG TGGACATGCC CTCGGCCATA CGCCTGGCCA AAAGACTGCA CTCCCTCGAC 
GGTTTCAAGA AGAGCGATGT GTCGCGACAC CTCAGCAAGA ACAATGACTT TAGTCGAGCG 
GTGGCCGATG AGTATCTCAA ACATTTTACC TTTGAGAAGA AGTCACTTGA CCAAGCGCTG 
CGTGAGTTCT TGCAGCAATT CTCGCTGTCC GGCGAAACGC AGGAACGGGA ACGAGTGCTG 
GTGCACTTTT CCAAGCGCTT CCTCGACTGC AATCCTGGCA CCTTTAACTC GCAGGACGCC 
GTGCACACGC TGACCTGTGC CATAATGTTG CTGAATACGG ACTTGCACGG CCAGAACATA 
AATCGTAAGA TGAGCTGTGC GGAATTCGTC GACAACCTGG CAGATCTCAA CGATGGCGAG 
AACTTTCCCA AGGATGTGCT CAAGTCGCTT TACCAGGCCA TTAAGACCAA GCCGCTTGAG 
TGGGCACTAG ATGAAGAGGC AGGTGATCTG CAGCAGCAAA GAGCCAACAA TAGTGCCCTG 
GGCAATGTGG GTCTCAATCC CTTCCTCGAT CCCCCAGAAC TGGCCACAGC TGTGGAATAC 
AAAAAAGGCT ATGTGATGCG TAAATGCTGC TATGACAGCA GCTTTAAGAA GACTCCCTTT 
GGCAAACGCT CCTGGAAGAT GTTCTACTGC ACGCTGCGTG ATCTTGTGCT GTATTTGCAT 
AAGGATGAGC ACGGTTTTCG TAAAAGTCAA ATGTCCGACA ATCTGCACAA TGCAATACGC 
ATACATCACG CACTGGCCAC CAAGGCCAAC GACTACACCA AGAAGCAACA TGTGTTCCGG 
CTGCAGACGG CCGACCAGGC CGAGTATCTG TTCCAGACCA GCGACTCCAA GGAGCTGCAG 
TCGTGGGTGG AGACGATAAA TTACGTGTGC GCCGCTATAT CAGCGCCTCC GCTCGAGGGA 
GGGGTGGGCA GTCAGAAACG ATTCCAACGT CCGCTCCTGC CCAGCAAACA ATCCAAGCTG 
ATGCTGAAAG AGCAGTTGGA CTCGCACGAG GTGCAGCTGG CGCAGTTGGA TCAGGAGCTT 
AACGAGCACA AGAAGGGTCC TATTCCCAGC AAGGGCTTGG CTCTGCAGAA CTACAAGGAA 
AAGGAGAGCT ACTTGCAGTA TGAACTTCGT CGCTACCGCA CTTATGTGAG CATTTTGAGC 
GCCAAGATGC TGGCTGATCA GCAGCAGCTG GAGCTGCAGG CGCAGCAGCC CTCTCCAGCG 
TCGCACGAGG AAGAGGCCGA CACATTCCCA GTGGGCACCA CCGCCTGCAC GCCACCCACG 
CCGCAAAGTA TTAACCAGAA GGATCAGCAG AAGGAGCAGC AGCAGCAACA GCCAACGAAC 
AGAAAAGAGA AGAAAAAGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097873.1
CDS	527..4690
Translation	MSEELKVVLRRSEQHSGFGFSLLGTTGPPHVIYDIVENSPAADCGAVEAGDVILKVNGTDVHRYTTKEVLKCLRLSEQLVTLELKRDPKLKARIKEQLANTQSPHYVDIESPNIYDYHSSSTNSSPNHRPNAGGKGAATTPSQTGLRYKSPTHLPSLRQNSSPLLASGSTTTTTTATHTHSHSRNSSASSTKIKVVETSITTSTTNVVGLTSPTGSVGGGVGGEATSPTFRPSRIPQALTKCAVPKPVPVLHSPQNKRPRPSQIPTKAANGNGNGHTAHLPPQSLQHSNSYSGSPVTRQRFADREPEREPEPNSAPPQPAKAPRFEAYMMTGDLILNLSRTPQTSNPLPAQAKKIDSLRDSPSRLVNPRINGALAPRASGESSPTSSSSVDSPTNTSSDSVKREAKLLQKQQQQQQQTYQQQQQRDSINNSYNRKDSLTNDTLLMCEELEPDEEGEYVLEEDNKQQRQRQQQQRYRQQQNQQRYEYYQNEDELEEQEEVEEEREEDQTHYDITNIETYQSGVGRGDDDDSDRQCLVDDDDDDDAYDDEENDAGDEDYSTNSLGSGSAKQRLRALKQRTATRQQQRNRDAVDCAGRSGSGSSSTTVKSEAGGLGLDETSFSVPTSPISLSTPLIDKETANSVPTSPEPSSLVPESSSGAGAGAVVVRRHNGHVVRKCDAAGFRTSKSEDHLQQIQREGIAAVIPIDIDEDVNSSLNTLLDTRQDSEDSQASDRDRIVWTYNAPLQPHQLAALQRQQQQQEQQFQQQQQQLHQQHLQQQQQLQQQHQQQQQQQQQQQQQQLYGGMVLSDPSDSDSTILVSDAAAHQRQQLKQQLRAQQQQQRERERDRDRDREQSEHKVVIQVRGLDSNSSGGNGTNGRSEEDVVTLTDEPLGTMTVGMRDASPPVSDDGSDVESLHSYHYSPKAVDMPSAIRLAKRLHSLDGFKKSDVSRHLSKNNDFSRAVADEYLKHFTFEKKSLDQALREFLQQFSLSGETQERERVLVHFSKRFLDCNPGTFNSQDAVHTLTCAIMLLNTDLHGQNINRKMSCAEFVDNLADLNDGENFPKDVLKSLYQAIKTKPLEWALDEEAGDLQQQRANNSALGNVGLNPFLDPPELATAVEYKKGYVMRKCCYDSSFKKTPFGKRSWKMFYCTLRDLVLYLHKDEHGFRKSQMSDNLHNAIRIHHALATKANDYTKKQHVFRLQTADQAEYLFQTSDSKELQSWVETINYVCAAISAPPLEGGVGSQKRFQRPLLPSKQSKLMLKEQLDSHEVQLAQLDQELNEHKKGPIPSKGLALQNYKEKESYLQYELRRYRTYVSILSAKMLADQQQLELQAQQPSPASHEEEADTFPVGTTACTPPTPQSINQKDQQKEQQQQQPTNRKEKKKK

Target ORF information:

RefSeq Version	NM_001104403.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster exchange factor for Arf 6, transcript variant C (Efa6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104403.2

ORF Insert Sequence:

ATGAGCGAGG AACTGAAAGT GGTGCTGCGG CGCAGCGAGC AGCATTCCGG TTTCGGGTTT 
TCGCTACTCG GAACCACCGG ACCGCCACAT GTCATCTACG ACATCGTCGA GAATTCGCCG 
GCAGCCGATT GCGGAGCGGT TGAGGCCGGG GATGTCATCC TCAAAGTCAA CGGAACGGAT 
GTCCATCGCT ATACAACGAA GGAAGTTCTC AAATGCCTGC GCCTGTCGGA ACAGCTGGTG 
ACCTTGGAGC TGAAGCGAGA TCCCAAGCTC AAGGCGCGTA TCAAAGAGCA GCTGGCCAAC 
ACCCAGAGTC CGCACTATGT GGACATTGAG TCGCCGAACA TCTACGATTA CCACAGCAGC 
AGCACCAACT CCTCGCCGAA TCACCGGCCG AACGCTGGTG GCAAGGGGGC GGCGACCACG 
CCCTCGCAGA CCGGACTGCG CTACAAGTCA CCGACGCACT TGCCCTCGCT GCGCCAGAAT 
TCGTCGCCAC TGCTGGCGAG TGGATCCACC ACGACCACAA CCACCGCCAC GCATACGCAC 
AGCCACAGTC GAAACTCCTC GGCCAGCTCC ACCAAGATTA AGGTGGTGGA GACAAGCATC 
ACCACCTCGA CCACGAACGT AGTGGGCCTC ACATCGCCCA CCGGTAGTGT TGGGGGTGGT 
GTTGGTGGGG AGGCCACTTC GCCCACCTTC CGCCCCTCAC GCATTCCACA GGCGCTCACC 
AAATGTGCCG TACCCAAGCC CGTGCCCGTT CTCCATTCGC CGCAGAATAA GCGGCCACGC 
CCATCGCAGA TTCCGACAAA GGCGGCGAAC GGAAACGGGA ACGGACATAC CGCCCATCTG 
CCACCGCAAT CGTTGCAGCA TTCCAACAGC TACAGCGGCA GTCCGGTGAC TCGGCAGCGG 
TTTGCCGATA GGGAGCCGGA GCGGGAACCG GAACCGAATT CGGCGCCACC GCAGCCGGCG 
AAGGCGCCAC GCTTTGAGGC TTACATGATG ACCGGTGACC TTATTCTCAA TCTGTCCCGG 
ACACCGCAGA CCAGCAATCC GCTTCCCGCC CAGGCCAAAA AGATTGACAG TCTTCGCGAT 
TCACCCAGTC GTTTGGTAAA TCCGCGTATC AACGGCGCAC TAGCACCGCG TGCCTCTGGT 
GAATCCTCGC CCACGTCCTC CTCTTCGGTA GACTCGCCCA CCAACACCAG CTCAGATTCC 
GTGAAGCGCG AGGCAAAGCT GCTGCAGAAA CAACAGCAGC AGCAGCAACA AACCTACCAA 
CAACAACAGC AGCGGGACAG CATCAACAAC AGCTACAATC GTAAGGATTC GCTGACCAAC 
GATACGCTGC TCATGTGCGA GGAGTTGGAG CCGGACGAGG AGGGCGAGTA TGTCCTGGAA 
GAGGACAACA AACAGCAGCG GCAGCGCCAA CAGCAACAAC GATACCGCCA GCAGCAAAAT 
CAGCAACGCT ACGAATACTA CCAAAACGAG GACGAGCTGG AGGAGCAGGA GGAGGTTGAG 
GAGGAGCGCG AGGAGGATCA AACACACTAC GACATCACCA ATATTGAAAC CTACCAGAGC 
GGTGTGGGCC GGGGTGACGA CGATGACAGT GATCGGCAGT GTCTGGTAGA CGACGACGAT 
GACGATGATG CCTACGATGA TGAGGAGAAC GATGCGGGCG ACGAGGACTA CTCCACCAAC 
TCGCTGGGCT CCGGTTCAGC CAAGCAACGA TTGCGGGCGT TGAAACAGCG CACTGCCACC 
CGCCAGCAGC AGCGAAATCG GGATGCCGTC GACTGTGCAG GACGCTCCGG ATCGGGATCA 
TCATCTACCA CGGTCAAGAG CGAGGCTGGC GGCCTGGGCC TAGATGAAAC CTCCTTCTCA 
GTGCCAACCT CTCCGATCTC GCTGTCGACG CCGCTGATCG ACAAGGAGAC GGCCAACTCG 
GTGCCCACGA GCCCGGAGCC CAGTTCGCTT GTTCCGGAGT CGAGCAGTGG CGCTGGCGCC 
GGAGCCGTGG TGGTGCGCCG GCATAACGGA CATGTGGTGC GGAAGTGTGA TGCCGCCGGT 
TTCCGCACCA GCAAGTCTGA GGACCATTTG CAGCAGATCC AGCGCGAGGG CATCGCCGCT 
GTGATACCCA TCGACATTGA TGAGGATGTG AATAGTTCGC TGAACACGCT GCTGGACACG 
CGTCAGGACT CCGAGGACTC GCAGGCATCG GATCGCGATC GGATCGTGTG GACTTATAAT 
GCGCCACTCC AGCCGCATCA GTTGGCGGCT CTTCAGCGAC AGCAGCAACA GCAAGAGCAG 
CAATTCCAAC AGCAGCAGCA GCAGCTTCAC CAGCAACATC TGCAGCAACA GCAGCAGCTT 
CAGCAGCAAC ATCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAGCA GCAACTCTAC 
GGCGGCATGG TACTTAGCGA TCCCAGTGAT TCGGACTCCA CCATCCTCGT CTCGGACGCG 
GCCGCCCATC AGCGCCAGCA GCTCAAGCAG CAGTTGCGCG CCCAGCAGCA ACAGCAGAGA 
GAAAGGGAAC GGGATAGGGA TCGAGACAGG GAACAGTCCG AGCACAAAGT GGTCATACAA 
GTGCGCGGTC TGGACAGTAA CAGCAGCGGC GGCAACGGAA CTAACGGCCG CTCCGAGGAG 
GATGTGGTCA CGCTGACGGA CGAGCCGCTG GGCACGATGA CCGTTGGCAT GCGGGACGCC 
TCGCCGCCAG TCTCTGACGA TGGCAGCGAT GTGGAGTCCC TGCACTCGTA TCACTACTCG 
CCCAAGGCCG TGGACATGCC CTCGGCCATA CGCCTGGCCA AAAGACTGCA CTCCCTCGAC 
GGTTTCAAGA AGAGCGATGT GTCGCGACAC CTCAGCAAGA ACAATGACTT TAGTCGAGCG 
GTGGCCGATG AGTATCTCAA ACATTTTACC TTTGAGAAGA AGTCACTTGA CCAAGCGCTG 
CGTGAGTTCT TGCAGCAATT CTCGCTGTCC GGCGAAACGC AGGAACGGGA ACGAGTGCTG 
GTGCACTTTT CCAAGCGCTT CCTCGACTGC AATCCTGGCA CCTTTAACTC GCAGGACGCC 
GTGCACACGC TGACCTGTGC CATAATGTTG CTGAATACGG ACTTGCACGG CCAGAACATA 
AATCGTAAGA TGAGCTGTGC GGAATTCGTC GACAACCTGG CAGATCTCAA CGATGGCGAG 
AACTTTCCCA AGGATGTGCT CAAGTCGCTT TACCAGGCCA TTAAGACCAA GCCGCTTGAG 
TGGGCACTAG ATGAAGAGGC AGGTGATCTG CAGCAGCAAA GAGCCAACAA TAGTGCCCTG 
GGCAATGTGG GTCTCAATCC CTTCCTCGAT CCCCCAGAAC TGGCCACAGC TGTGGAATAC 
AAAAAAGGCT ATGTGATGCG TAAATGCTGC TATGACAGCA GCTTTAAGAA GACTCCCTTT 
GGCAAACGCT CCTGGAAGAT GTTCTACTGC ACGCTGCGTG ATCTTGTGCT GTATTTGCAT 
AAGGATGAGC ACGGTTTTCG TAAAAGTCAA ATGTCCGACA ATCTGCACAA TGCAATACGC 
ATACATCACG CACTGGCCAC CAAGGCCAAC GACTACACCA AGAAGCAACA TGTGTTCCGG 
CTGCAGACGG CCGACCAGGC CGAGTATCTG TTCCAGACCA GCGACTCCAA GGAGCTGCAG 
TCGTGGGTGG AGACGATAAA TTACGTGTGC GCCGCTATAT CAGCGCCTCC GCTCGAGGGA 
GGGGTGGGCA GTCAGAAACG ATTCCAACGT CCGCTCCTGC CCAGCAAACA ATCCAAGCTG 
ATGCTGAAAG AGCAGTTGGA CTCGCACGAG GTGCAGCTGG CGCAGTTGGA TCAGGAGCTT 
AACGAGCACA AGAAGGGTCC TATTCCCAGC AAGGGCTTGG CTCTGCAGAA CTACAAGGAA 
AAGGAGAGCT ACTTGCAGTA TGAACTTCGT CGCTACCGCA CTTATGTGAG CATTTTGAGC 
GCCAAGATGC TGGCTGATCA GCAGCAGCTG GAGCTGCAGG CGCAGCAGCC CTCTCCAGCG 
TCGCACGAGG AAGAGGCCGA CACATTCCCA GTGGGCACCA CCGCCTGCAC GCCACCCACG 
CCGCAAAGTA TTAACCAGAA GGATCAGCAG AAGGAGCAGC AGCAGCAACA GCCAACGAAC 
AGAAAAGAGA AGAAAAAGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Efa6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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