CG31233 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG31233
Entrez Gene ID	42557
Full Name	CG31233 gene product from transcript CG31233-RB
Synonyms	CG5839,Dmel\CG31233,anon-WO0140519.125,anon-WO0140519.65
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 93F8-93F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_169965.2	NP_732653.2	uncharacterized protein, isoform A
NM_001316609.1	NP_001303538.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG31233	NP_732653.2
Drosophila melanogaster (fruit fly)	CG31343	NP_732652.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG31233 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG31233 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05617	NM_001316609.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG31233), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa05617	NM_169965.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG31233), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG31233 ( NM_169965.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732653.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG31233), mRNA.

Price & Availability↑

CloneID	OFa05617
Clone ID Related Accession (Same CDS sequence)	NM_169965.2 , NM_001316609.1
Accession Version	NM_169965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169965.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTGCG TTCAGGTCGG GGGCATGTCA GTATTCTTGG TCCTTTTGCT GGCCATTTCC 
GCCACCCAGG CGGCTGTGGT TCGCCAATAT CCGATCCTTG ATCACCTGCA ACATCTGGAG 
AATCCCATGC AGCGATTGGT GGTTCCTCTC GCCGACGAGG ATAACTACCG TCTGCCCTAC 
GATACGATCC CGAGTCATTA TGCCGTGTCC TTGAGCACAA ATGTGCACAC GGGAGACACC 
GTGTTCAATG GTACCGTGGC CATCACCTTA AGCGTTTTGA ACACCACCAC AAAAATTGTG 
GTCCACGCCC GCCAGCTGGA GAACTTTACA GCCTCCATTA TTCAGCAGGG AGTCACTGAA 
GCCGTGGCCC AGGAATTGGT ATACGAATAT GAAGCAGAGC GCGAGTTTCT TACCTTCAGC 
AAGACGGGAC TGACTTTCCC CGAAGACACC ACATGGATTC TGACAATCAA CTACCAGGGA 
CATCTGCGCA CAGACAACGG AGGATTCTAC CTATCCACAT ATACGGATGA GGAGGGGAAC 
ACCAAGTATC TGGCCACCAC CCAGTTTGAG TCCACTGACG CACGGCACGC CTTCCCCTGC 
TACGATGAAC CCTCGAAGAG GGCGGAGTTC ACTATCACCA TTAAGCACGA TCCAAGCTAC 
AATGCCATCA GCAATATGCC AGTCGATTCG AGCAGCACCT CTGGAGTCAC CGTCTTCCAA 
AAGACCGTAA ATATGCCATC CTACCTGGTC GCCTTTATTG TGTCCGAGTT TGTCTTCTCG 
GAGGGTGAGC TAAATGGCTT GCCACAGCGC GTCTTCTCCC GCAATGGAAC CGAACATGAG 
CAGGAGTGGG CTCTAACCAC CGGAATGCTG GTGGAGAAGC GTCTGTCCGG CTACTTTGAT 
GTTCCCTTCG CCCTGCCCAA GCTAGATCAG GCTGGTATCC CGGACTTTGC CGCCGGTGCC 
ATGGAGAACT GGGGTCTGGC CACGTACCGT GAGGAGTACC TGTTGTACAA CACGGAAAAC 
TCAACCACCA GCACGCAGAC CAACATAGCT ACCATTGAGG CGCACGAGGA TGCCCACATG 
TGGTTTGGTG ATCTGGTGGC CATCGAATGG TGGAGCTTCC TTTGGCTAAA GGAAGGTTTC 
GCCACCCTTT TCGAGAACTT GGCTGTGGAC TTGGCCTATC CCGAGTGGGA CATTTTCCAG 
ACCTTCCACG CTGGATCGTA CCAGAGCGCT CTGGTGAATG ATGCCAGCGC TAATGCCCGC 
CCCATGAGCC ACTTTGTCCA GAAGCCCTCG GAGATTGCCC TGCTCTATGA CTCCGTTTCG 
TATGCTAAGG CCGGATCCGT TCTTGACATG TGGCGCCATG CCCTGACTAA CACCGTTTTC 
CAACGCGGCC TACACAACTA TCTGGTCGAC AATCAATTCA CTGCTGCCAA TCCCAGCAAG 
CTTTTCGAGG CCATTGCCAA GGCTGCCGTG GAGGAGAACT ATGAAGTGAA AGCAACCGTG 
CCCGATATGA TGGGTAGCTG GACAAACCAG GGAGGAGTTC CTTTACTGAC CGTGACTCGT 
AACTACGACA ACGGAAGCTT CACAGTTAAG CAGTCGGTGT ACACCAACGA TAAGGACTAT 
ACGAATGACA AGTTGTGGTA TGTGCCCATT AACTACGCCG AATCGAAAAA TCCCGACTTC 
CGCAACACCG AGGCTACTCA TTATCTGCTT AACCAATCCG AGATTACCAT CTATTCCGAC 
TTGGACCAGA CCAACTGGTT GATTCTGAAC AAAAAATCCA CTGGTTACTA CCGCGTGCTC 
TATGATGCCC AAAACTATCA ACTGATCACC AGCGCATTGA TCACTCGTCC CCACAAGATC 
GATCCTAGGA ACCGAGCCCA GTTGATTAAC GACTTGTACC GCTTTGCAAC CAGTGGCCGT 
GTGCCCCATG CCACTTTGCT GGAGCTGTTG ACCTACTTGC CCCAGGAGGA TCAGTACTCT 
CCCTGGTCCG CTACCAACAC AGTGATTACC CTGCTCAACC GCTACCTGAG CGGAGATGCG 
GATTATGGCA ACTTCCAGTT CTACGTCCGT GAACTGGTGA GCCTGCAGTT CGACAAGTTC 
GGAGTGAATG ATAACAAAGG TGACCACCAT TTGGTACCAT TCACCCGCAA TGTGCTAATT 
AACCTTGCTT GTCTGGCGGG TCTGGAGAAT TGTCTGACGG AGACCACGGC CAAGTTGACG 
GCTCTCGTGG AGGAGGGAAC TAAGATCGAG CCGAATCTAC AGTCGCAGGT GTACTGCAAC 
GGCCTGAAGC AGGCCGATGA CAAAACCTTT GACTTTGTGT TCAACAAGCT GCTGAACTCC 
ACCGATCAGG CCGAGCGTCG TCTTCTGATC TCCGCCCTGG GCTGCTCCCA GAACACTGCC 
CAGCTAGAGA AGTTCGTGTA CAGCAGCATT GAGCCAGAGG GTTTGAGGGA TCAGGAAAGG 
ATCACGGTGC TGAGTCCGGT CTACTCCCGC GGCGAGGTGG GTCTGCTGGT CGCCATTGAG 
TTCCTGCAGA AGAACTGGGA AAAGTATGGT CAGCTAAATT CCGGCTTTGG TGGCGTAAAT 
CCTCTGTACA GCGACATTGT GGGTATTTCC GCCTATACCG TCAACGACAA GCAGAAGGAT 
GCCTTGCAAG AGTTGGTGGA CACTGTCAAG GGTTCTGAGT ATGTGCCCAG CACTCTGCAG 
GATAGCGTGG ATAGCAACGT GAATGCCAAC TACGCGTGGC TGGCTTCCAA CAGGGATCCC 
CTGATGACCT GGGTCGCCGG ATTCCGCAGC GGCAGCTCCG CCCTGAACGC CTCCATTTTG 
GCAATCCTTG CCTGCCTTCT GGCCGCCAAG CTATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732653.2
CDS	24..2882
Translation	MSCVQVGGMSVFLVLLLAISATQAAVVRQYPILDHLQHLENPMQRLVVPLADEDNYRLPYDTIPSHYAVSLSTNVHTGDTVFNGTVAITLSVLNTTTKIVVHARQLENFTASIIQQGVTEAVAQELVYEYEAEREFLTFSKTGLTFPEDTTWILTINYQGHLRTDNGGFYLSTYTDEEGNTKYLATTQFESTDARHAFPCYDEPSKRAEFTITIKHDPSYNAISNMPVDSSSTSGVTVFQKTVNMPSYLVAFIVSEFVFSEGELNGLPQRVFSRNGTEHEQEWALTTGMLVEKRLSGYFDVPFALPKLDQAGIPDFAAGAMENWGLATYREEYLLYNTENSTTSTQTNIATIEAHEDAHMWFGDLVAIEWWSFLWLKEGFATLFENLAVDLAYPEWDIFQTFHAGSYQSALVNDASANARPMSHFVQKPSEIALLYDSVSYAKAGSVLDMWRHALTNTVFQRGLHNYLVDNQFTAANPSKLFEAIAKAAVEENYEVKATVPDMMGSWTNQGGVPLLTVTRNYDNGSFTVKQSVYTNDKDYTNDKLWYVPINYAESKNPDFRNTEATHYLLNQSEITIYSDLDQTNWLILNKKSTGYYRVLYDAQNYQLITSALITRPHKIDPRNRAQLINDLYRFATSGRVPHATLLELLTYLPQEDQYSPWSATNTVITLLNRYLSGDADYGNFQFYVRELVSLQFDKFGVNDNKGDHHLVPFTRNVLINLACLAGLENCLTETTAKLTALVEEGTKIEPNLQSQVYCNGLKQADDKTFDFVFNKLLNSTDQAERRLLISALGCSQNTAQLEKFVYSSIEPEGLRDQERITVLSPVYSRGEVGLLVAIEFLQKNWEKYGQLNSGFGGVNPLYSDIVGISAYTVNDKQKDALQELVDTVKGSEYVPSTLQDSVDSNVNANYAWLASNRDPLMTWVAGFRSGSSALNASILAILACLLAAKLF

Target ORF information:

RefSeq Version	NM_169965.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG31233), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169965.2

ORF Insert Sequence:

ATGAGTTGCG TTCAGGTCGG GGGCATGTCA GTATTCTTGG TCCTTTTGCT GGCCATTTCC 
GCCACCCAGG CGGCTGTGGT TCGCCAATAT CCGATCCTTG ATCACCTGCA ACATCTGGAG 
AATCCCATGC AGCGATTGGT GGTTCCTCTC GCCGACGAGG ATAACTACCG TCTGCCCTAC 
GATACGATCC CGAGTCATTA TGCCGTGTCC TTGAGCACAA ATGTGCACAC GGGAGACACC 
GTGTTCAATG GTACCGTGGC CATCACCTTA AGCGTTTTGA ACACCACCAC AAAAATTGTG 
GTCCACGCCC GCCAGCTGGA GAACTTTACA GCCTCCATTA TTCAGCAGGG AGTCACTGAA 
GCCGTGGCCC AGGAATTGGT ATACGAATAT GAAGCAGAGC GCGAGTTTCT TACCTTCAGC 
AAGACGGGAC TGACTTTCCC CGAAGACACC ACATGGATTC TGACAATCAA CTACCAGGGA 
CATCTGCGCA CAGACAACGG AGGATTCTAC CTATCCACAT ATACGGATGA GGAGGGGAAC 
ACCAAGTATC TGGCCACCAC CCAGTTTGAG TCCACTGACG CACGGCACGC CTTCCCCTGC 
TACGATGAAC CCTCGAAGAG GGCGGAGTTC ACTATCACCA TTAAGCACGA TCCAAGCTAC 
AATGCCATCA GCAATATGCC AGTCGATTCG AGCAGCACCT CTGGAGTCAC CGTCTTCCAA 
AAGACCGTAA ATATGCCATC CTACCTGGTC GCCTTTATTG TGTCCGAGTT TGTCTTCTCG 
GAGGGTGAGC TAAATGGCTT GCCACAGCGC GTCTTCTCCC GCAATGGAAC CGAACATGAG 
CAGGAGTGGG CTCTAACCAC CGGAATGCTG GTGGAGAAGC GTCTGTCCGG CTACTTTGAT 
GTTCCCTTCG CCCTGCCCAA GCTAGATCAG GCTGGTATCC CGGACTTTGC CGCCGGTGCC 
ATGGAGAACT GGGGTCTGGC CACGTACCGT GAGGAGTACC TGTTGTACAA CACGGAAAAC 
TCAACCACCA GCACGCAGAC CAACATAGCT ACCATTGAGG CGCACGAGGA TGCCCACATG 
TGGTTTGGTG ATCTGGTGGC CATCGAATGG TGGAGCTTCC TTTGGCTAAA GGAAGGTTTC 
GCCACCCTTT TCGAGAACTT GGCTGTGGAC TTGGCCTATC CCGAGTGGGA CATTTTCCAG 
ACCTTCCACG CTGGATCGTA CCAGAGCGCT CTGGTGAATG ATGCCAGCGC TAATGCCCGC 
CCCATGAGCC ACTTTGTCCA GAAGCCCTCG GAGATTGCCC TGCTCTATGA CTCCGTTTCG 
TATGCTAAGG CCGGATCCGT TCTTGACATG TGGCGCCATG CCCTGACTAA CACCGTTTTC 
CAACGCGGCC TACACAACTA TCTGGTCGAC AATCAATTCA CTGCTGCCAA TCCCAGCAAG 
CTTTTCGAGG CCATTGCCAA GGCTGCCGTG GAGGAGAACT ATGAAGTGAA AGCAACCGTG 
CCCGATATGA TGGGTAGCTG GACAAACCAG GGAGGAGTTC CTTTACTGAC CGTGACTCGT 
AACTACGACA ACGGAAGCTT CACAGTTAAG CAGTCGGTGT ACACCAACGA TAAGGACTAT 
ACGAATGACA AGTTGTGGTA TGTGCCCATT AACTACGCCG AATCGAAAAA TCCCGACTTC 
CGCAACACCG AGGCTACTCA TTATCTGCTT AACCAATCCG AGATTACCAT CTATTCCGAC 
TTGGACCAGA CCAACTGGTT GATTCTGAAC AAAAAATCCA CTGGTTACTA CCGCGTGCTC 
TATGATGCCC AAAACTATCA ACTGATCACC AGCGCATTGA TCACTCGTCC CCACAAGATC 
GATCCTAGGA ACCGAGCCCA GTTGATTAAC GACTTGTACC GCTTTGCAAC CAGTGGCCGT 
GTGCCCCATG CCACTTTGCT GGAGCTGTTG ACCTACTTGC CCCAGGAGGA TCAGTACTCT 
CCCTGGTCCG CTACCAACAC AGTGATTACC CTGCTCAACC GCTACCTGAG CGGAGATGCG 
GATTATGGCA ACTTCCAGTT CTACGTCCGT GAACTGGTGA GCCTGCAGTT CGACAAGTTC 
GGAGTGAATG ATAACAAAGG TGACCACCAT TTGGTACCAT TCACCCGCAA TGTGCTAATT 
AACCTTGCTT GTCTGGCGGG TCTGGAGAAT TGTCTGACGG AGACCACGGC CAAGTTGACG 
GCTCTCGTGG AGGAGGGAAC TAAGATCGAG CCGAATCTAC AGTCGCAGGT GTACTGCAAC 
GGCCTGAAGC AGGCCGATGA CAAAACCTTT GACTTTGTGT TCAACAAGCT GCTGAACTCC 
ACCGATCAGG CCGAGCGTCG TCTTCTGATC TCCGCCCTGG GCTGCTCCCA GAACACTGCC 
CAGCTAGAGA AGTTCGTGTA CAGCAGCATT GAGCCAGAGG GTTTGAGGGA TCAGGAAAGG 
ATCACGGTGC TGAGTCCGGT CTACTCCCGC GGCGAGGTGG GTCTGCTGGT CGCCATTGAG 
TTCCTGCAGA AGAACTGGGA AAAGTATGGT CAGCTAAATT CCGGCTTTGG TGGCGTAAAT 
CCTCTGTACA GCGACATTGT GGGTATTTCC GCCTATACCG TCAACGACAA GCAGAAGGAT 
GCCTTGCAAG AGTTGGTGGA CACTGTCAAG GGTTCTGAGT ATGTGCCCAG CACTCTGCAG 
GATAGCGTGG ATAGCAACGT GAATGCCAAC TACGCGTGGC TGGCTTCCAA CAGGGATCCC 
CTGATGACCT GGGTCGCCGG ATTCCGCAGC GGCAGCTCCG CCCTGAACGC CTCCATTTTG 
GCAATCCTTG CCTGCCTTCT GGCCGCCAAG CTATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG31233 ( NM_001316609.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303538.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG31233), mRNA.

Price & Availability↑

CloneID	OFa05617
Clone ID Related Accession (Same CDS sequence)	NM_169965.2 , NM_001316609.1
Accession Version	NM_001316609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTGCG TTCAGGTCGG GGGCATGTCA GTATTCTTGG TCCTTTTGCT GGCCATTTCC 
GCCACCCAGG CGGCTGTGGT TCGCCAATAT CCGATCCTTG ATCACCTGCA ACATCTGGAG 
AATCCCATGC AGCGATTGGT GGTTCCTCTC GCCGACGAGG ATAACTACCG TCTGCCCTAC 
GATACGATCC CGAGTCATTA TGCCGTGTCC TTGAGCACAA ATGTGCACAC GGGAGACACC 
GTGTTCAATG GTACCGTGGC CATCACCTTA AGCGTTTTGA ACACCACCAC AAAAATTGTG 
GTCCACGCCC GCCAGCTGGA GAACTTTACA GCCTCCATTA TTCAGCAGGG AGTCACTGAA 
GCCGTGGCCC AGGAATTGGT ATACGAATAT GAAGCAGAGC GCGAGTTTCT TACCTTCAGC 
AAGACGGGAC TGACTTTCCC CGAAGACACC ACATGGATTC TGACAATCAA CTACCAGGGA 
CATCTGCGCA CAGACAACGG AGGATTCTAC CTATCCACAT ATACGGATGA GGAGGGGAAC 
ACCAAGTATC TGGCCACCAC CCAGTTTGAG TCCACTGACG CACGGCACGC CTTCCCCTGC 
TACGATGAAC CCTCGAAGAG GGCGGAGTTC ACTATCACCA TTAAGCACGA TCCAAGCTAC 
AATGCCATCA GCAATATGCC AGTCGATTCG AGCAGCACCT CTGGAGTCAC CGTCTTCCAA 
AAGACCGTAA ATATGCCATC CTACCTGGTC GCCTTTATTG TGTCCGAGTT TGTCTTCTCG 
GAGGGTGAGC TAAATGGCTT GCCACAGCGC GTCTTCTCCC GCAATGGAAC CGAACATGAG 
CAGGAGTGGG CTCTAACCAC CGGAATGCTG GTGGAGAAGC GTCTGTCCGG CTACTTTGAT 
GTTCCCTTCG CCCTGCCCAA GCTAGATCAG GCTGGTATCC CGGACTTTGC CGCCGGTGCC 
ATGGAGAACT GGGGTCTGGC CACGTACCGT GAGGAGTACC TGTTGTACAA CACGGAAAAC 
TCAACCACCA GCACGCAGAC CAACATAGCT ACCATTGAGG CGCACGAGGA TGCCCACATG 
TGGTTTGGTG ATCTGGTGGC CATCGAATGG TGGAGCTTCC TTTGGCTAAA GGAAGGTTTC 
GCCACCCTTT TCGAGAACTT GGCTGTGGAC TTGGCCTATC CCGAGTGGGA CATTTTCCAG 
ACCTTCCACG CTGGATCGTA CCAGAGCGCT CTGGTGAATG ATGCCAGCGC TAATGCCCGC 
CCCATGAGCC ACTTTGTCCA GAAGCCCTCG GAGATTGCCC TGCTCTATGA CTCCGTTTCG 
TATGCTAAGG CCGGATCCGT TCTTGACATG TGGCGCCATG CCCTGACTAA CACCGTTTTC 
CAACGCGGCC TACACAACTA TCTGGTCGAC AATCAATTCA CTGCTGCCAA TCCCAGCAAG 
CTTTTCGAGG CCATTGCCAA GGCTGCCGTG GAGGAGAACT ATGAAGTGAA AGCAACCGTG 
CCCGATATGA TGGGTAGCTG GACAAACCAG GGAGGAGTTC CTTTACTGAC CGTGACTCGT 
AACTACGACA ACGGAAGCTT CACAGTTAAG CAGTCGGTGT ACACCAACGA TAAGGACTAT 
ACGAATGACA AGTTGTGGTA TGTGCCCATT AACTACGCCG AATCGAAAAA TCCCGACTTC 
CGCAACACCG AGGCTACTCA TTATCTGCTT AACCAATCCG AGATTACCAT CTATTCCGAC 
TTGGACCAGA CCAACTGGTT GATTCTGAAC AAAAAATCCA CTGGTTACTA CCGCGTGCTC 
TATGATGCCC AAAACTATCA ACTGATCACC AGCGCATTGA TCACTCGTCC CCACAAGATC 
GATCCTAGGA ACCGAGCCCA GTTGATTAAC GACTTGTACC GCTTTGCAAC CAGTGGCCGT 
GTGCCCCATG CCACTTTGCT GGAGCTGTTG ACCTACTTGC CCCAGGAGGA TCAGTACTCT 
CCCTGGTCCG CTACCAACAC AGTGATTACC CTGCTCAACC GCTACCTGAG CGGAGATGCG 
GATTATGGCA ACTTCCAGTT CTACGTCCGT GAACTGGTGA GCCTGCAGTT CGACAAGTTC 
GGAGTGAATG ATAACAAAGG TGACCACCAT TTGGTACCAT TCACCCGCAA TGTGCTAATT 
AACCTTGCTT GTCTGGCGGG TCTGGAGAAT TGTCTGACGG AGACCACGGC CAAGTTGACG 
GCTCTCGTGG AGGAGGGAAC TAAGATCGAG CCGAATCTAC AGTCGCAGGT GTACTGCAAC 
GGCCTGAAGC AGGCCGATGA CAAAACCTTT GACTTTGTGT TCAACAAGCT GCTGAACTCC 
ACCGATCAGG CCGAGCGTCG TCTTCTGATC TCCGCCCTGG GCTGCTCCCA GAACACTGCC 
CAGCTAGAGA AGTTCGTGTA CAGCAGCATT GAGCCAGAGG GTTTGAGGGA TCAGGAAAGG 
ATCACGGTGC TGAGTCCGGT CTACTCCCGC GGCGAGGTGG GTCTGCTGGT CGCCATTGAG 
TTCCTGCAGA AGAACTGGGA AAAGTATGGT CAGCTAAATT CCGGCTTTGG TGGCGTAAAT 
CCTCTGTACA GCGACATTGT GGGTATTTCC GCCTATACCG TCAACGACAA GCAGAAGGAT 
GCCTTGCAAG AGTTGGTGGA CACTGTCAAG GGTTCTGAGT ATGTGCCCAG CACTCTGCAG 
GATAGCGTGG ATAGCAACGT GAATGCCAAC TACGCGTGGC TGGCTTCCAA CAGGGATCCC 
CTGATGACCT GGGTCGCCGG ATTCCGCAGC GGCAGCTCCG CCCTGAACGC CTCCATTTTG 
GCAATCCTTG CCTGCCTTCT GGCCGCCAAG CTATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303538.1
CDS	138..2996
Translation	MSCVQVGGMSVFLVLLLAISATQAAVVRQYPILDHLQHLENPMQRLVVPLADEDNYRLPYDTIPSHYAVSLSTNVHTGDTVFNGTVAITLSVLNTTTKIVVHARQLENFTASIIQQGVTEAVAQELVYEYEAEREFLTFSKTGLTFPEDTTWILTINYQGHLRTDNGGFYLSTYTDEEGNTKYLATTQFESTDARHAFPCYDEPSKRAEFTITIKHDPSYNAISNMPVDSSSTSGVTVFQKTVNMPSYLVAFIVSEFVFSEGELNGLPQRVFSRNGTEHEQEWALTTGMLVEKRLSGYFDVPFALPKLDQAGIPDFAAGAMENWGLATYREEYLLYNTENSTTSTQTNIATIEAHEDAHMWFGDLVAIEWWSFLWLKEGFATLFENLAVDLAYPEWDIFQTFHAGSYQSALVNDASANARPMSHFVQKPSEIALLYDSVSYAKAGSVLDMWRHALTNTVFQRGLHNYLVDNQFTAANPSKLFEAIAKAAVEENYEVKATVPDMMGSWTNQGGVPLLTVTRNYDNGSFTVKQSVYTNDKDYTNDKLWYVPINYAESKNPDFRNTEATHYLLNQSEITIYSDLDQTNWLILNKKSTGYYRVLYDAQNYQLITSALITRPHKIDPRNRAQLINDLYRFATSGRVPHATLLELLTYLPQEDQYSPWSATNTVITLLNRYLSGDADYGNFQFYVRELVSLQFDKFGVNDNKGDHHLVPFTRNVLINLACLAGLENCLTETTAKLTALVEEGTKIEPNLQSQVYCNGLKQADDKTFDFVFNKLLNSTDQAERRLLISALGCSQNTAQLEKFVYSSIEPEGLRDQERITVLSPVYSRGEVGLLVAIEFLQKNWEKYGQLNSGFGGVNPLYSDIVGISAYTVNDKQKDALQELVDTVKGSEYVPSTLQDSVDSNVNANYAWLASNRDPLMTWVAGFRSGSSALNASILAILACLLAAKLF

Target ORF information:

RefSeq Version	NM_001316609.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG31233), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316609.1

ORF Insert Sequence:

ATGAGTTGCG TTCAGGTCGG GGGCATGTCA GTATTCTTGG TCCTTTTGCT GGCCATTTCC 
GCCACCCAGG CGGCTGTGGT TCGCCAATAT CCGATCCTTG ATCACCTGCA ACATCTGGAG 
AATCCCATGC AGCGATTGGT GGTTCCTCTC GCCGACGAGG ATAACTACCG TCTGCCCTAC 
GATACGATCC CGAGTCATTA TGCCGTGTCC TTGAGCACAA ATGTGCACAC GGGAGACACC 
GTGTTCAATG GTACCGTGGC CATCACCTTA AGCGTTTTGA ACACCACCAC AAAAATTGTG 
GTCCACGCCC GCCAGCTGGA GAACTTTACA GCCTCCATTA TTCAGCAGGG AGTCACTGAA 
GCCGTGGCCC AGGAATTGGT ATACGAATAT GAAGCAGAGC GCGAGTTTCT TACCTTCAGC 
AAGACGGGAC TGACTTTCCC CGAAGACACC ACATGGATTC TGACAATCAA CTACCAGGGA 
CATCTGCGCA CAGACAACGG AGGATTCTAC CTATCCACAT ATACGGATGA GGAGGGGAAC 
ACCAAGTATC TGGCCACCAC CCAGTTTGAG TCCACTGACG CACGGCACGC CTTCCCCTGC 
TACGATGAAC CCTCGAAGAG GGCGGAGTTC ACTATCACCA TTAAGCACGA TCCAAGCTAC 
AATGCCATCA GCAATATGCC AGTCGATTCG AGCAGCACCT CTGGAGTCAC CGTCTTCCAA 
AAGACCGTAA ATATGCCATC CTACCTGGTC GCCTTTATTG TGTCCGAGTT TGTCTTCTCG 
GAGGGTGAGC TAAATGGCTT GCCACAGCGC GTCTTCTCCC GCAATGGAAC CGAACATGAG 
CAGGAGTGGG CTCTAACCAC CGGAATGCTG GTGGAGAAGC GTCTGTCCGG CTACTTTGAT 
GTTCCCTTCG CCCTGCCCAA GCTAGATCAG GCTGGTATCC CGGACTTTGC CGCCGGTGCC 
ATGGAGAACT GGGGTCTGGC CACGTACCGT GAGGAGTACC TGTTGTACAA CACGGAAAAC 
TCAACCACCA GCACGCAGAC CAACATAGCT ACCATTGAGG CGCACGAGGA TGCCCACATG 
TGGTTTGGTG ATCTGGTGGC CATCGAATGG TGGAGCTTCC TTTGGCTAAA GGAAGGTTTC 
GCCACCCTTT TCGAGAACTT GGCTGTGGAC TTGGCCTATC CCGAGTGGGA CATTTTCCAG 
ACCTTCCACG CTGGATCGTA CCAGAGCGCT CTGGTGAATG ATGCCAGCGC TAATGCCCGC 
CCCATGAGCC ACTTTGTCCA GAAGCCCTCG GAGATTGCCC TGCTCTATGA CTCCGTTTCG 
TATGCTAAGG CCGGATCCGT TCTTGACATG TGGCGCCATG CCCTGACTAA CACCGTTTTC 
CAACGCGGCC TACACAACTA TCTGGTCGAC AATCAATTCA CTGCTGCCAA TCCCAGCAAG 
CTTTTCGAGG CCATTGCCAA GGCTGCCGTG GAGGAGAACT ATGAAGTGAA AGCAACCGTG 
CCCGATATGA TGGGTAGCTG GACAAACCAG GGAGGAGTTC CTTTACTGAC CGTGACTCGT 
AACTACGACA ACGGAAGCTT CACAGTTAAG CAGTCGGTGT ACACCAACGA TAAGGACTAT 
ACGAATGACA AGTTGTGGTA TGTGCCCATT AACTACGCCG AATCGAAAAA TCCCGACTTC 
CGCAACACCG AGGCTACTCA TTATCTGCTT AACCAATCCG AGATTACCAT CTATTCCGAC 
TTGGACCAGA CCAACTGGTT GATTCTGAAC AAAAAATCCA CTGGTTACTA CCGCGTGCTC 
TATGATGCCC AAAACTATCA ACTGATCACC AGCGCATTGA TCACTCGTCC CCACAAGATC 
GATCCTAGGA ACCGAGCCCA GTTGATTAAC GACTTGTACC GCTTTGCAAC CAGTGGCCGT 
GTGCCCCATG CCACTTTGCT GGAGCTGTTG ACCTACTTGC CCCAGGAGGA TCAGTACTCT 
CCCTGGTCCG CTACCAACAC AGTGATTACC CTGCTCAACC GCTACCTGAG CGGAGATGCG 
GATTATGGCA ACTTCCAGTT CTACGTCCGT GAACTGGTGA GCCTGCAGTT CGACAAGTTC 
GGAGTGAATG ATAACAAAGG TGACCACCAT TTGGTACCAT TCACCCGCAA TGTGCTAATT 
AACCTTGCTT GTCTGGCGGG TCTGGAGAAT TGTCTGACGG AGACCACGGC CAAGTTGACG 
GCTCTCGTGG AGGAGGGAAC TAAGATCGAG CCGAATCTAC AGTCGCAGGT GTACTGCAAC 
GGCCTGAAGC AGGCCGATGA CAAAACCTTT GACTTTGTGT TCAACAAGCT GCTGAACTCC 
ACCGATCAGG CCGAGCGTCG TCTTCTGATC TCCGCCCTGG GCTGCTCCCA GAACACTGCC 
CAGCTAGAGA AGTTCGTGTA CAGCAGCATT GAGCCAGAGG GTTTGAGGGA TCAGGAAAGG 
ATCACGGTGC TGAGTCCGGT CTACTCCCGC GGCGAGGTGG GTCTGCTGGT CGCCATTGAG 
TTCCTGCAGA AGAACTGGGA AAAGTATGGT CAGCTAAATT CCGGCTTTGG TGGCGTAAAT 
CCTCTGTACA GCGACATTGT GGGTATTTCC GCCTATACCG TCAACGACAA GCAGAAGGAT 
GCCTTGCAAG AGTTGGTGGA CACTGTCAAG GGTTCTGAGT ATGTGCCCAG CACTCTGCAG 
GATAGCGTGG ATAGCAACGT GAATGCCAAC TACGCGTGGC TGGCTTCCAA CAGGGATCCC 
CTGATGACCT GGGTCGCCGG ATTCCGCAGC GGCAGCTCCG CCCTGAACGC CTCCATTTTG 
GCAATCCTTG CCTGCCTTCT GGCCGCCAAG CTATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG31233 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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