cdi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	cdi
Entrez Gene ID	42289
Full Name	center divider
Synonyms	91F,B9.3,CDI/TSKI,CG6027,Cdi,Cdi/TESK1,Dmel\CG6027,cdi/TESK1,l(3)07013
General protein information	Preferred Names center divider Names CG6027 gene product from transcript CG6027-RB 91F gene CG6027-PA CG6027-PB cdi-PA cdi-PB central divider centre divider centrer divider
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91E4-91F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260262.2	NP_001247191.1	center divider, isoform B
NM_079677.4	NP_524401.2	center divider, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	cdi	NP_524401.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004112	XP_312990.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show interference with the function of the ADF/cofilin phosphatase Slingshot (ssh), which antagonizes Cdi, also results in a suppression of signaling triggered by the Sevenless RTK.
Title: The Cdi/TESK1 kinase is required for Sevenless signaling and epithelial organization in the Drosophila eye.

A screen for modifiers of rotundracgap gain-of-function in the eye revealed cdi as a downstream effector of Rac1 during spermatogenesis.
Title: A screen for modifiers of RacGAP(84C) gain-of-function in the Drosophila eye revealed the LIM kinase Cdi/TESK1 as a downstream effector of Rac1 during spermatogenesis.

The following cdi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cdi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa07803	NM_001260262.2 Latest version!	Drosophila melanogaster center divider (cdi), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa05371	NM_079677.4 Latest version!	Drosophila melanogaster center divider (cdi), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cdi ( NM_001260262.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247191.1 Drosophila melanogaster center divider (cdi), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa07803
Clone ID Related Accession (Same CDS sequence)	NM_001260262.2
Accession Version	NM_001260262.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	center divider, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260262.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCCTGA ATCAGCACAG TGGACCCGGC CCGCCGTCGG AGATCTGCGG ATTATCGGTG 
GACCAGGTGG ACTCATCGGC CGCCTCCCCT TTCATCTGGC CGAGCACAGC GGGTATGTGC 
ACCACCGCCA CCACCGTCAC AACATCGACC AATGGAGACG CCACCGCGGA AGCACCAGTG 
AAACATCAGC CGCTCCACAA CGGCGGCTGG ATCGGAAATG GTCTTCCGCC GGCGCCCGTG 
ACGCGGACCA TATCATCGGA TCGCCTGGTC ACCGGCAGCT CGTGTCGGGC GCTGCGCACT 
GCCGTCTCAG CATTATACTC CGTGGATGAC TTCGTCAAGG AGAAAATTGG ATCGGGATTC 
TTCTCGGAGG TTTACAAGGT CACACATCGT ACAACCGGCC AGGTGATGGT GCTCAAGATG 
AATCAGTTGC GGGCCAATCG ACCGAATATG CTGCGGGAGG TGCAGCTGTT GAATAAACTT 
TCGCATGCGA ATATATTGAG CTTCATGGGC GTTTGCGTGC AGGAGGGCCA ACTGCACGCC 
CTGACCGAGT ACATCAACGG CGGCTCCCTG GAGCAGCTGC TGGCCAACAA GGAGGTGGTG 
CTCAGCGCCA CGCAGAAGAT AAGACTGGCG CTGGGTATAG CGCGCGGCAT GTCCTACGTT 
CACGACGCGG GCATCTTTCA CAGGGATCTG ACGAGCAAGA ATGTACTTAT TCGCAATCTG 
GCCAACGATC AGTACGAAGC TGTAGTGGGT GACTTTGGTC TAGCCGCAAA GATACCAGTC 
AAATCAAAAT CACGCCTTGA GACAGTCGGC TCACCGTATT GGGTCAGTCC GGAGTGCCTG 
AAGGGCCAGT GGTACGATCA GACAAGCGAT GTCTTCTCCT TTGGCATCAT TCAATGCGAG 
ATCATAGCCC GCATCGAAGC GGACCCGGAT ATGATGCCGC GCACGGCGTC GTTTGGACTG 
GACTACCTGG CATTTGTAGA ATTGTGTCCG ATGGACACTC CTCCTGTGTT TTTACGTCTG 
GCCTTCTACT GCTGTCTGTA CGATGCCAAG AGCCGACCCA CGTTCCACGA TGCCACCAAA 
AAGTTGACTT TGCTGCTGGA GAAATACGAG CACGAGTCGT CGGCCGGAGC AAGTCCACTC 
AGTTCCCTGG AGTTGATCAA CTGCTCACCA GGAGGCAGCG ATTCGGAGAA CGTAACGTTG 
ACACCCCGAG CATCAGTACC CACGGTGGCC ACTATTTGTA CTGCTCCTGC TGGCACTTTA 
ACACCGCGCC ATCTACAGGA AGTGGACGAG GACGGCTTCC GATTTCCAAG TAAAAAGTCT 
GCTTCGGCTG CGCTTCCGGG CACGCCAAAT GGATCTGCTG GAAGCAGTGG CTTTGATGAT 
CATATACTGA AGTCTAGAGT GCGACGGGCT ACTCAGAAGT TGGAGGCCGA GATCTTGTTG 
CACCGGCGTT CGCTAAGCGA GAACATCATC CACTTCCCCA TGCACACGTC GCCAAGTGAC 
AAGGCGCGGT GTCATCAGAT GCAGCGACAG CGCTCCTCAA CACACTCACC AACGCCCCCA 
AGGCAGCTTT CCTCGGTTCA GTTGCCCGCC ACGTCTATTC CAGATCCCCC ACCGCTTCCC 
AAGCCCGTTC CAGCGGTACT CACACTGCGC AAAGTGGGCG ATCCACAGTA CAAGCCGACA 
TCTGAAAAGG ATCACAACGG CTCTAAACCC AATCCGTTTG AAGCGCTGGC GCAACTGCGT 
GGTGTCAAGA AAATCCTGGA CGCCAATCCG AAAACCTATG CAGCAGGGGT GGGAGATCTA 
TTCAGCTCAT GCTTCGAGAT GCTAGCGCCG TTCTTCAAAG AACTGGCCGC AATGCAGAGC 
AAAGCGGGGG CTCAGGGCGG AGGAACGAGT ACGGAGGAAA CATCAACGGG TAACAAGGCT 
TCGGCAGCAA CGCCCAGTGA ACCAAAGAGC CTGCCAGTGT CGCCGCATAT GACGCGCAAA 
TACAGCGCCA TCGAGCTTCG ACTTCCTCAG CGAATCAGCA ATGCTGCTCC TCCAGCAGTT 
ACCAGTGCTC CACCACCATC AATAGCATTG CCAACCGATG AGGATGACTG CGATGATTGC 
AATTCGGAAG GTGAGGATCC CGATGGCGGT GGCAACTGTG CAGATGCGGA ATCAAGCAGC 
GATGACAGCG CCGTGCGTCC GTCCACTGTG CCAACCGCTC GAAAATACCG CGCGAATTCC 
TTGTATGCGC ATCCGCTTTT TCGCGGCTCC AATGGCACCA GCATGAAAAG TACCAGTACT 
GGTGAATTCG AGAGTCCAAC TCTACTGAAG GCCAGCGCAT CTACAATCAC GTTGACTGCG 
TCAGTGGGTG CGACCAATTC GTGTGAGGAT CTGACTGAAA GCGCCAGAAA ATCAAAAGAG 
CAAGCGAGTA GCGGTGCAAC AGCGGCGGTG CAGGCTTTGC CCACTCCCAT GTCTGGCTGC 
TCCACCAGGT TGGTGGAAAC CCCGGTGTCC GTTTCATCCT GCACTGATCT TGAGCTCGAC 
GAACTGAAAA ACACCTGCGA TCTGAACGAA TTGAAGAACT CGTGTGAGCA GCTTCCCTTG 
AAGACAAACC TAACGAGGCG GGACTCTATA GAAAGTGGCT TCTTCTCGTG CTTTAACGAA 
GAGTCCGATG CGGCGATAAC CAACAACAGT TTCGGACGGC AGCTGAACGG GATTGGATCG 
TTGGGGTTTG GTTGCGGCGG CGGGGGCAAC AGTAGCAACT GCTGCTATGA GAAATTGAAG 
ACGCAGCTTA TGCTCGATGC ATGCGTCAGC GCAGATAGTG AAATCAAGTT AAACACAGCC 
ACGGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247191.1
CDS	1101..3929
Translation	MSLNQHSGPGPPSEICGLSVDQVDSSAASPFIWPSTAGMCTTATTVTTSTNGDATAEAPVKHQPLHNGGWIGNGLPPAPVTRTISSDRLVTGSSCRALRTAVSALYSVDDFVKEKIGSGFFSEVYKVTHRTTGQVMVLKMNQLRANRPNMLREVQLLNKLSHANILSFMGVCVQEGQLHALTEYINGGSLEQLLANKEVVLSATQKIRLALGIARGMSYVHDAGIFHRDLTSKNVLIRNLANDQYEAVVGDFGLAAKIPVKSKSRLETVGSPYWVSPECLKGQWYDQTSDVFSFGIIQCEIIARIEADPDMMPRTASFGLDYLAFVELCPMDTPPVFLRLAFYCCLYDAKSRPTFHDATKKLTLLLEKYEHESSAGASPLSSLELINCSPGGSDSENVTLTPRASVPTVATICTAPAGTLTPRHLQEVDEDGFRFPSKKSASAALPGTPNGSAGSSGFDDHILKSRVRRATQKLEAEILLHRRSLSENIIHFPMHTSPSDKARCHQMQRQRSSTHSPTPPRQLSSVQLPATSIPDPPPLPKPVPAVLTLRKVGDPQYKPTSEKDHNGSKPNPFEALAQLRGVKKILDANPKTYAAGVGDLFSSCFEMLAPFFKELAAMQSKAGAQGGGTSTEETSTGNKASAATPSEPKSLPVSPHMTRKYSAIELRLPQRISNAAPPAVTSAPPPSIALPTDEDDCDDCNSEGEDPDGGGNCADAESSSDDSAVRPSTVPTARKYRANSLYAHPLFRGSNGTSMKSTSTGEFESPTLLKASASTITLTASVGATNSCEDLTESARKSKEQASSGATAAVQALPTPMSGCSTRLVETPVSVSSCTDLELDELKNTCDLNELKNSCEQLPLKTNLTRRDSIESGFFSCFNEESDAAITNNSFGRQLNGIGSLGFGCGGGGNSSNCCYEKLKTQLMLDACVSADSEIKLNTATV

Target ORF information:

RefSeq Version	NM_001260262.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster center divider (cdi), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260262.2

ORF Insert Sequence:

ATGAGCCTGA ATCAGCACAG TGGACCCGGC CCGCCGTCGG AGATCTGCGG ATTATCGGTG 
GACCAGGTGG ACTCATCGGC CGCCTCCCCT TTCATCTGGC CGAGCACAGC GGGTATGTGC 
ACCACCGCCA CCACCGTCAC AACATCGACC AATGGAGACG CCACCGCGGA AGCACCAGTG 
AAACATCAGC CGCTCCACAA CGGCGGCTGG ATCGGAAATG GTCTTCCGCC GGCGCCCGTG 
ACGCGGACCA TATCATCGGA TCGCCTGGTC ACCGGCAGCT CGTGTCGGGC GCTGCGCACT 
GCCGTCTCAG CATTATACTC CGTGGATGAC TTCGTCAAGG AGAAAATTGG ATCGGGATTC 
TTCTCGGAGG TTTACAAGGT CACACATCGT ACAACCGGCC AGGTGATGGT GCTCAAGATG 
AATCAGTTGC GGGCCAATCG ACCGAATATG CTGCGGGAGG TGCAGCTGTT GAATAAACTT 
TCGCATGCGA ATATATTGAG CTTCATGGGC GTTTGCGTGC AGGAGGGCCA ACTGCACGCC 
CTGACCGAGT ACATCAACGG CGGCTCCCTG GAGCAGCTGC TGGCCAACAA GGAGGTGGTG 
CTCAGCGCCA CGCAGAAGAT AAGACTGGCG CTGGGTATAG CGCGCGGCAT GTCCTACGTT 
CACGACGCGG GCATCTTTCA CAGGGATCTG ACGAGCAAGA ATGTACTTAT TCGCAATCTG 
GCCAACGATC AGTACGAAGC TGTAGTGGGT GACTTTGGTC TAGCCGCAAA GATACCAGTC 
AAATCAAAAT CACGCCTTGA GACAGTCGGC TCACCGTATT GGGTCAGTCC GGAGTGCCTG 
AAGGGCCAGT GGTACGATCA GACAAGCGAT GTCTTCTCCT TTGGCATCAT TCAATGCGAG 
ATCATAGCCC GCATCGAAGC GGACCCGGAT ATGATGCCGC GCACGGCGTC GTTTGGACTG 
GACTACCTGG CATTTGTAGA ATTGTGTCCG ATGGACACTC CTCCTGTGTT TTTACGTCTG 
GCCTTCTACT GCTGTCTGTA CGATGCCAAG AGCCGACCCA CGTTCCACGA TGCCACCAAA 
AAGTTGACTT TGCTGCTGGA GAAATACGAG CACGAGTCGT CGGCCGGAGC AAGTCCACTC 
AGTTCCCTGG AGTTGATCAA CTGCTCACCA GGAGGCAGCG ATTCGGAGAA CGTAACGTTG 
ACACCCCGAG CATCAGTACC CACGGTGGCC ACTATTTGTA CTGCTCCTGC TGGCACTTTA 
ACACCGCGCC ATCTACAGGA AGTGGACGAG GACGGCTTCC GATTTCCAAG TAAAAAGTCT 
GCTTCGGCTG CGCTTCCGGG CACGCCAAAT GGATCTGCTG GAAGCAGTGG CTTTGATGAT 
CATATACTGA AGTCTAGAGT GCGACGGGCT ACTCAGAAGT TGGAGGCCGA GATCTTGTTG 
CACCGGCGTT CGCTAAGCGA GAACATCATC CACTTCCCCA TGCACACGTC GCCAAGTGAC 
AAGGCGCGGT GTCATCAGAT GCAGCGACAG CGCTCCTCAA CACACTCACC AACGCCCCCA 
AGGCAGCTTT CCTCGGTTCA GTTGCCCGCC ACGTCTATTC CAGATCCCCC ACCGCTTCCC 
AAGCCCGTTC CAGCGGTACT CACACTGCGC AAAGTGGGCG ATCCACAGTA CAAGCCGACA 
TCTGAAAAGG ATCACAACGG CTCTAAACCC AATCCGTTTG AAGCGCTGGC GCAACTGCGT 
GGTGTCAAGA AAATCCTGGA CGCCAATCCG AAAACCTATG CAGCAGGGGT GGGAGATCTA 
TTCAGCTCAT GCTTCGAGAT GCTAGCGCCG TTCTTCAAAG AACTGGCCGC AATGCAGAGC 
AAAGCGGGGG CTCAGGGCGG AGGAACGAGT ACGGAGGAAA CATCAACGGG TAACAAGGCT 
TCGGCAGCAA CGCCCAGTGA ACCAAAGAGC CTGCCAGTGT CGCCGCATAT GACGCGCAAA 
TACAGCGCCA TCGAGCTTCG ACTTCCTCAG CGAATCAGCA ATGCTGCTCC TCCAGCAGTT 
ACCAGTGCTC CACCACCATC AATAGCATTG CCAACCGATG AGGATGACTG CGATGATTGC 
AATTCGGAAG GTGAGGATCC CGATGGCGGT GGCAACTGTG CAGATGCGGA ATCAAGCAGC 
GATGACAGCG CCGTGCGTCC GTCCACTGTG CCAACCGCTC GAAAATACCG CGCGAATTCC 
TTGTATGCGC ATCCGCTTTT TCGCGGCTCC AATGGCACCA GCATGAAAAG TACCAGTACT 
GGTGAATTCG AGAGTCCAAC TCTACTGAAG GCCAGCGCAT CTACAATCAC GTTGACTGCG 
TCAGTGGGTG CGACCAATTC GTGTGAGGAT CTGACTGAAA GCGCCAGAAA ATCAAAAGAG 
CAAGCGAGTA GCGGTGCAAC AGCGGCGGTG CAGGCTTTGC CCACTCCCAT GTCTGGCTGC 
TCCACCAGGT TGGTGGAAAC CCCGGTGTCC GTTTCATCCT GCACTGATCT TGAGCTCGAC 
GAACTGAAAA ACACCTGCGA TCTGAACGAA TTGAAGAACT CGTGTGAGCA GCTTCCCTTG 
AAGACAAACC TAACGAGGCG GGACTCTATA GAAAGTGGCT TCTTCTCGTG CTTTAACGAA 
GAGTCCGATG CGGCGATAAC CAACAACAGT TTCGGACGGC AGCTGAACGG GATTGGATCG 
TTGGGGTTTG GTTGCGGCGG CGGGGGCAAC AGTAGCAACT GCTGCTATGA GAAATTGAAG 
ACGCAGCTTA TGCTCGATGC ATGCGTCAGC GCAGATAGTG AAATCAAGTT AAACACAGCC 
ACGGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cdi ( NM_079677.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524401.2 Drosophila melanogaster center divider (cdi), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05371
Clone ID Related Accession (Same CDS sequence)	NM_079677.4
Accession Version	NM_079677.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	center divider, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_079677.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCCTGA ATCAGCACAG TGGACCCGGC CCGCCGTCGG AGATCTGCGG ATTATCGGTG 
GACCAGGTGG ACTCATCGGC CGCCTCCCCT TTCATCTGGC CGAGCACAGC GGGTATGTGC 
ACCACCGCCA CCACCGTCAC AACATCGACC AATGGAGACG CCACCGCGGA AGCACCAGTG 
AAACATCAGC CGCTCCACAA CGGCGGCTGG ATCGGAAATG GTCTTCCGCC GGCGCCCGTG 
ACGCGGACCA TATCATCGGA TCGCCTGGTC ACCGGCAGCT CGTGTCGGGC GCTGCGCACT 
GCCGTCTCAG CATTATACTC CGTGGATGAC TTCGTCAAGG AGAAAATTGG ATCGGGATTC 
TTCTCGGAGG TTTACAAGGT CACACATCGT ACAACCGGCC AGGTGATGGT GCTCAAGATG 
AATCAGTTGC GGGCCAATCG ACCGAATATG CTGCGGGAGG TGCAGCTGTT GAATAAACTT 
TCGCATGCGA ATATATTGAG CTTCATGGGC GTTTGCGTGC AGGAGGGCCA ACTGCACGCC 
CTGACCGAGT ACATCAACGG CGGCTCCCTG GAGCAGCTGC TGGCCAACAA GGAGGTGGTG 
CTCAGCGCCA CGCAGAAGAT AAGACTGGCG CTGGGTATAG CGCGCGGCAT GTCCTACGTT 
CACGACGCGG GCATCTTTCA CAGGGATCTG ACGAGCAAGA ATGTACTTAT TCGCAATCTG 
GCCAACGATC AGTACGAAGC TGTAGTGGGT GACTTTGGTC TAGCCGCAAA GATACCAGTC 
AAATCCAGAA AATCACGCCT TGAGACAGTC GGCTCACCGT ATTGGGTCAG TCCGGAGTGC 
CTGAAGGGCC AGTGGTACGA TCAGACAAGC GATGTCTTCT CCTTTGGCAT CATTCAATGC 
GAGATCATAG CCCGCATCGA AGCGGACCCG GATATGATGC CGCGCACGGC GTCGTTTGGA 
CTGGACTACC TGGCATTTGT AGAATTGTGT CCGATGGACA CTCCTCCTGT GTTTTTACGT 
CTGGCCTTCT ACTGCTGTCT GTACGATGCC AAGAGCCGAC CCACGTTCCA CGATGCCACC 
AAAAAGTTGA CTTTGCTGCT GGAGAAATAC GAGCACGAGT CGTCGGCCGG AGCAAGTCCA 
CTCAGTTCCC TGGAGTTGAT CAACTGCTCA CCAGGAGGCA GCGATTCGGA GAACGTAACG 
TTGACACCCC GAGCATCAGT ACCCACGGTG GCCACTATTT GTACTGCTCC TGCTGGCACT 
TTAACACCGC GCCATCTACA GGAAGTGGAC GAGGACGGCT TCCGATTTCC AAGTAAAAAG 
TCTGCTTCGG CTGCGCTTCC GGGCACGCCA AATGGATCTG CTGGAAGCAG TGGCTTTGAT 
GATCATATAC TGAAGTCTAG AGTGCGACGG GCTACTCAGA AGTTGGAGGC CGAGATCTTG 
TTGCACCGGC GTTCGCTAAG CGAGAACATC ATCCACTTCC CCATGCACAC GTCGCCAAGT 
GACAAGGCGC GGTGTCATCA GATGCAGCGA CAGCGCTCCT CAACACACTC ACCAACGCCC 
CCAAGGCAGC TTTCCTCGGT TCAGTTGCCC GCCACGTCTA TTCCAGATCC CCCACCGCTT 
CCCAAGCCCG TTCCAGCGGT ACTCACACTG CGCAAAGTGG GCGATCCACA GTACAAGCCG 
ACATCTGAAA AGGATCACAA CGGCTCTAAA CCCAATCCGT TTGAAGCGCT GGCGCAACTG 
CGTGGTGTCA AGAAAATCCT GGACGCCAAT CCGAAAACCT ATGCAGCAGG GGTGGGAGAT 
CTATTCAGCT CATGCTTCGA GATGCTAGCG CCGTTCTTCA AAGAACTGGC CGCAATGCAG 
AGCAAAGCGG GGGCTCAGGG CGGAGGAACG AGTACGGAGG AAACATCAAC GGGTAACAAG 
GCTTCGGCAG CAACGCCCAG TGAACCAAAG AGCCTGCCAG TGTCGCCGCA TATGACGCGC 
AAATACAGCG CCATCGAGCT TCGACTTCCT CAGCGAATCA GCAATGCTGC TCCTCCAGCA 
GTTACCAGTG CTCCACCACC ATCAATAGCA TTGCCAACCG ATGAGGATGA CTGCGATGAT 
TGCAATTCGG AAGGTGAGGA TCCCGATGGC GGTGGCAACT GTGCAGATGC GGAATCAAGC 
AGCGATGACA GCGCCGTGCG TCCGTCCACT GTGCCAACCG CTCGAAAATA CCGCGCGAAT 
TCCTTGTATG CGCATCCGCT TTTTCGCGGC TCCAATGGCA CCAGCATGAA AAGTACCAGT 
ACTGGTGAAT TCGAGAGTCC AACTCTACTG AAGGCCAGCG CATCTACAAT CACGTTGACT 
GCGTCAGTGG GTGCGACCAA TTCGTGTGAG GATCTGACTG AAAGCGCCAG AAAATCAAAA 
GAGCAAGCGA GTAGCGGTGC AACAGCGGCG GTGCAGGCTT TGCCCACTCC CATGTCTGGC 
TGCTCCACCA GGTTGGTGGA AACCCCGGTG TCCGTTTCAT CCTGCACTGA TCTTGAGCTC 
GACGAACTGA AAAACACCTG CGATCTGAAC GAATTGAAGA ACTCGTGTGA GCAGCTTCCC 
TTGAAGACAA ACCTAACGAG GCGGGACTCT ATAGAAAGTG GCTTCTTCTC GTGCTTTAAC 
GAAGAGTCCG ATGCGGCGAT AACCAACAAC AGTTTCGGAC GGCAGCTGAA CGGGATTGGA 
TCGTTGGGGT TTGGTTGCGG CGGCGGGGGC AACAGTAGCA ACTGCTGCTA TGAGAAATTG 
AAGACGCAGC TTATGCTCGA TGCATGCGTC AGCGCAGATA GTGAAATCAA CGATAAACTG 
GCGAGTTTGT GTCGCTGCTG CTACTACGCC ACCTCGACAT CGAATGCCGC CGTAACTGCA 
GCCGCTGCTG CGGCCCAGCA ACAACAGCAG AGCCACTTGC TCAACGATAC GTCCTCGACC 
TCATCGTCGG TGCTGCTGAT CGACAATGAT CCCGCTGTGA TCGGTGGAAA TACCTCATCA 
TCCACAGCGT TGGACTCGAT GGATGACTTG GACGCGGACA TTGTGGCATC GCGGCGCACA 
CACCAGCGAC GATACACTTG CCACCATGCG TTGCTCAGCG GGAATGGCAG CGAATCCCAA 
ATGGCGGCTA CGGCGAGCCT GATCAATCGT CTGGCTTTAG ACTCGGAGAT ACATACTCTG 
CTTCAGCGTA GTCCTTTTGC AACGAATCAA CTATTGTACT GTAAGAACCG CACCTCGTCC 
ATATACACGG ATAGCTCCGA TGATATAAGT AGCTTGGCCG GCTCTGACTC CCTGCTCTGG 
GACGACCGCT CCTTCACGGC ACTGCCCAGT ACAAGATCCG CACAGATTGC CAAGATAGTG 
GAGTATTTTG AGCGAAAGCG ACAGGACTCC TCGCCGCCTT CGGTGCACCA TCGCAATGGG 
AACGGGACGG TAGCAGCTGC TGTGGCTGCC TCTGCCCTGG GTTCCAACTA CCTTTCGTAC 
GAGTCGCGTC GCTATTCGGA CTTCAAGCGG CACCTGAACG CGGATGGCCT GTGCTTCGAT 
CTGGACAAGA AGCCGGCGCA GCAAAGGATG ATGGTGTGCG AGGGTGCGGT CAAGTCGAAG 
CTTCCCATAT TCGATAAAAA GAAGCAACAG CAAGGCGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524401.2
CDS	1101..4742
Translation	MSLNQHSGPGPPSEICGLSVDQVDSSAASPFIWPSTAGMCTTATTVTTSTNGDATAEAPVKHQPLHNGGWIGNGLPPAPVTRTISSDRLVTGSSCRALRTAVSALYSVDDFVKEKIGSGFFSEVYKVTHRTTGQVMVLKMNQLRANRPNMLREVQLLNKLSHANILSFMGVCVQEGQLHALTEYINGGSLEQLLANKEVVLSATQKIRLALGIARGMSYVHDAGIFHRDLTSKNVLIRNLANDQYEAVVGDFGLAAKIPVKSRKSRLETVGSPYWVSPECLKGQWYDQTSDVFSFGIIQCEIIARIEADPDMMPRTASFGLDYLAFVELCPMDTPPVFLRLAFYCCLYDAKSRPTFHDATKKLTLLLEKYEHESSAGASPLSSLELINCSPGGSDSENVTLTPRASVPTVATICTAPAGTLTPRHLQEVDEDGFRFPSKKSASAALPGTPNGSAGSSGFDDHILKSRVRRATQKLEAEILLHRRSLSENIIHFPMHTSPSDKARCHQMQRQRSSTHSPTPPRQLSSVQLPATSIPDPPPLPKPVPAVLTLRKVGDPQYKPTSEKDHNGSKPNPFEALAQLRGVKKILDANPKTYAAGVGDLFSSCFEMLAPFFKELAAMQSKAGAQGGGTSTEETSTGNKASAATPSEPKSLPVSPHMTRKYSAIELRLPQRISNAAPPAVTSAPPPSIALPTDEDDCDDCNSEGEDPDGGGNCADAESSSDDSAVRPSTVPTARKYRANSLYAHPLFRGSNGTSMKSTSTGEFESPTLLKASASTITLTASVGATNSCEDLTESARKSKEQASSGATAAVQALPTPMSGCSTRLVETPVSVSSCTDLELDELKNTCDLNELKNSCEQLPLKTNLTRRDSIESGFFSCFNEESDAAITNNSFGRQLNGIGSLGFGCGGGGNSSNCCYEKLKTQLMLDACVSADSEINDKLASLCRCCYYATSTSNAAVTAAAAAAQQQQQSHLLNDTSSTSSSVLLIDNDPAVIGGNTSSSTALDSMDDLDADIVASRRTHQRRYTCHHALLSGNGSESQMAATASLINRLALDSEIHTLLQRSPFATNQLLYCKNRTSSIYTDSSDDISSLAGSDSLLWDDRSFTALPSTRSAQIAKIVEYFERKRQDSSPPSVHHRNGNGTVAAAVAASALGSNYLSYESRRYSDFKRHLNADGLCFDLDKKPAQQRMMVCEGAVKSKLPIFDKKKQQQGG

Target ORF information:

RefSeq Version	NM_079677.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster center divider (cdi), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079677.4

ORF Insert Sequence:

ATGAGCCTGA ATCAGCACAG TGGACCCGGC CCGCCGTCGG AGATCTGCGG ATTATCGGTG 
GACCAGGTGG ACTCATCGGC CGCCTCCCCT TTCATCTGGC CGAGCACAGC GGGTATGTGC 
ACCACCGCCA CCACCGTCAC AACATCGACC AATGGAGACG CCACCGCGGA AGCACCAGTG 
AAACATCAGC CGCTCCACAA CGGCGGCTGG ATCGGAAATG GTCTTCCGCC GGCGCCCGTG 
ACGCGGACCA TATCATCGGA TCGCCTGGTC ACCGGCAGCT CGTGTCGGGC GCTGCGCACT 
GCCGTCTCAG CATTATACTC CGTGGATGAC TTCGTCAAGG AGAAAATTGG ATCGGGATTC 
TTCTCGGAGG TTTACAAGGT CACACATCGT ACAACCGGCC AGGTGATGGT GCTCAAGATG 
AATCAGTTGC GGGCCAATCG ACCGAATATG CTGCGGGAGG TGCAGCTGTT GAATAAACTT 
TCGCATGCGA ATATATTGAG CTTCATGGGC GTTTGCGTGC AGGAGGGCCA ACTGCACGCC 
CTGACCGAGT ACATCAACGG CGGCTCCCTG GAGCAGCTGC TGGCCAACAA GGAGGTGGTG 
CTCAGCGCCA CGCAGAAGAT AAGACTGGCG CTGGGTATAG CGCGCGGCAT GTCCTACGTT 
CACGACGCGG GCATCTTTCA CAGGGATCTG ACGAGCAAGA ATGTACTTAT TCGCAATCTG 
GCCAACGATC AGTACGAAGC TGTAGTGGGT GACTTTGGTC TAGCCGCAAA GATACCAGTC 
AAATCCAGAA AATCACGCCT TGAGACAGTC GGCTCACCGT ATTGGGTCAG TCCGGAGTGC 
CTGAAGGGCC AGTGGTACGA TCAGACAAGC GATGTCTTCT CCTTTGGCAT CATTCAATGC 
GAGATCATAG CCCGCATCGA AGCGGACCCG GATATGATGC CGCGCACGGC GTCGTTTGGA 
CTGGACTACC TGGCATTTGT AGAATTGTGT CCGATGGACA CTCCTCCTGT GTTTTTACGT 
CTGGCCTTCT ACTGCTGTCT GTACGATGCC AAGAGCCGAC CCACGTTCCA CGATGCCACC 
AAAAAGTTGA CTTTGCTGCT GGAGAAATAC GAGCACGAGT CGTCGGCCGG AGCAAGTCCA 
CTCAGTTCCC TGGAGTTGAT CAACTGCTCA CCAGGAGGCA GCGATTCGGA GAACGTAACG 
TTGACACCCC GAGCATCAGT ACCCACGGTG GCCACTATTT GTACTGCTCC TGCTGGCACT 
TTAACACCGC GCCATCTACA GGAAGTGGAC GAGGACGGCT TCCGATTTCC AAGTAAAAAG 
TCTGCTTCGG CTGCGCTTCC GGGCACGCCA AATGGATCTG CTGGAAGCAG TGGCTTTGAT 
GATCATATAC TGAAGTCTAG AGTGCGACGG GCTACTCAGA AGTTGGAGGC CGAGATCTTG 
TTGCACCGGC GTTCGCTAAG CGAGAACATC ATCCACTTCC CCATGCACAC GTCGCCAAGT 
GACAAGGCGC GGTGTCATCA GATGCAGCGA CAGCGCTCCT CAACACACTC ACCAACGCCC 
CCAAGGCAGC TTTCCTCGGT TCAGTTGCCC GCCACGTCTA TTCCAGATCC CCCACCGCTT 
CCCAAGCCCG TTCCAGCGGT ACTCACACTG CGCAAAGTGG GCGATCCACA GTACAAGCCG 
ACATCTGAAA AGGATCACAA CGGCTCTAAA CCCAATCCGT TTGAAGCGCT GGCGCAACTG 
CGTGGTGTCA AGAAAATCCT GGACGCCAAT CCGAAAACCT ATGCAGCAGG GGTGGGAGAT 
CTATTCAGCT CATGCTTCGA GATGCTAGCG CCGTTCTTCA AAGAACTGGC CGCAATGCAG 
AGCAAAGCGG GGGCTCAGGG CGGAGGAACG AGTACGGAGG AAACATCAAC GGGTAACAAG 
GCTTCGGCAG CAACGCCCAG TGAACCAAAG AGCCTGCCAG TGTCGCCGCA TATGACGCGC 
AAATACAGCG CCATCGAGCT TCGACTTCCT CAGCGAATCA GCAATGCTGC TCCTCCAGCA 
GTTACCAGTG CTCCACCACC ATCAATAGCA TTGCCAACCG ATGAGGATGA CTGCGATGAT 
TGCAATTCGG AAGGTGAGGA TCCCGATGGC GGTGGCAACT GTGCAGATGC GGAATCAAGC 
AGCGATGACA GCGCCGTGCG TCCGTCCACT GTGCCAACCG CTCGAAAATA CCGCGCGAAT 
TCCTTGTATG CGCATCCGCT TTTTCGCGGC TCCAATGGCA CCAGCATGAA AAGTACCAGT 
ACTGGTGAAT TCGAGAGTCC AACTCTACTG AAGGCCAGCG CATCTACAAT CACGTTGACT 
GCGTCAGTGG GTGCGACCAA TTCGTGTGAG GATCTGACTG AAAGCGCCAG AAAATCAAAA 
GAGCAAGCGA GTAGCGGTGC AACAGCGGCG GTGCAGGCTT TGCCCACTCC CATGTCTGGC 
TGCTCCACCA GGTTGGTGGA AACCCCGGTG TCCGTTTCAT CCTGCACTGA TCTTGAGCTC 
GACGAACTGA AAAACACCTG CGATCTGAAC GAATTGAAGA ACTCGTGTGA GCAGCTTCCC 
TTGAAGACAA ACCTAACGAG GCGGGACTCT ATAGAAAGTG GCTTCTTCTC GTGCTTTAAC 
GAAGAGTCCG ATGCGGCGAT AACCAACAAC AGTTTCGGAC GGCAGCTGAA CGGGATTGGA 
TCGTTGGGGT TTGGTTGCGG CGGCGGGGGC AACAGTAGCA ACTGCTGCTA TGAGAAATTG 
AAGACGCAGC TTATGCTCGA TGCATGCGTC AGCGCAGATA GTGAAATCAA CGATAAACTG 
GCGAGTTTGT GTCGCTGCTG CTACTACGCC ACCTCGACAT CGAATGCCGC CGTAACTGCA 
GCCGCTGCTG CGGCCCAGCA ACAACAGCAG AGCCACTTGC TCAACGATAC GTCCTCGACC 
TCATCGTCGG TGCTGCTGAT CGACAATGAT CCCGCTGTGA TCGGTGGAAA TACCTCATCA 
TCCACAGCGT TGGACTCGAT GGATGACTTG GACGCGGACA TTGTGGCATC GCGGCGCACA 
CACCAGCGAC GATACACTTG CCACCATGCG TTGCTCAGCG GGAATGGCAG CGAATCCCAA 
ATGGCGGCTA CGGCGAGCCT GATCAATCGT CTGGCTTTAG ACTCGGAGAT ACATACTCTG 
CTTCAGCGTA GTCCTTTTGC AACGAATCAA CTATTGTACT GTAAGAACCG CACCTCGTCC 
ATATACACGG ATAGCTCCGA TGATATAAGT AGCTTGGCCG GCTCTGACTC CCTGCTCTGG 
GACGACCGCT CCTTCACGGC ACTGCCCAGT ACAAGATCCG CACAGATTGC CAAGATAGTG 
GAGTATTTTG AGCGAAAGCG ACAGGACTCC TCGCCGCCTT CGGTGCACCA TCGCAATGGG 
AACGGGACGG TAGCAGCTGC TGTGGCTGCC TCTGCCCTGG GTTCCAACTA CCTTTCGTAC 
GAGTCGCGTC GCTATTCGGA CTTCAAGCGG CACCTGAACG CGGATGGCCT GTGCTTCGAT 
CTGGACAAGA AGCCGGCGCA GCAAAGGATG ATGGTGTGCG AGGGTGCGGT CAAGTCGAAG 
CTTCCCATAT TCGATAAAAA GAAGCAACAG CAAGGCGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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cdi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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