Fas1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fas1
Entrez Gene ID	42025
Full Name	Fasciclin 1
Synonyms	CG6588,DROFAS,DROFAS1,DROFASI,Dmel\CG6588,Fas I,FasI,MRE24,fas I,fas-I,fas1,fasI,gasI
General protein information	Preferred Names Fasciclin 1 Names CG6588 gene product from transcript CG6588-RG CG6588-PA CG6588-PB CG6588-PC CG6588-PD CG6588-PE CG6588-PF CG6588-PG Fas1-PA Fas1-PB Fas1-PC Fas1-PD Fas1-PE Fas1-PF Fas1-PG Fasciclin-1 fasciclin 1 fasciclin I mRNA-like ncRNA in embryogenesis 24
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89D5-89D6

mRNA and Protein(s)

mRNA	Protein	Name
NM_169726.2	NP_732166.1	fasciclin 1, isoform A
NM_169724.2	NP_732164.1	fasciclin 1, isoform C
NM_001144594.2	NP_001138066.1	fasciclin 1, isoform F
NM_001144593.2	NP_001138065.1	fasciclin 1, isoform E
NM_001104344.2	NP_001097814.1	fasciclin 1, isoform D
NM_001275716.1	NP_001262645.1	fasciclin 1, isoform G
NM_169725.2	NP_732165.1	fasciclin 1, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000968	XP_309160.5
Drosophila melanogaster (fruit fly)	Fas1	NP_732165.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fas1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fas1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05150	NM_001104344.2 Latest version!	Drosophila melanogaster fasciclin 1, transcript variant D (Fas1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05151	NM_169724.2 Latest version!	Drosophila melanogaster fasciclin 1, transcript variant C (Fas1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05152	NM_169725.2 Latest version!	Drosophila melanogaster fasciclin 1, transcript variant B (Fas1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05153	NM_169726.2 Latest version!	Drosophila melanogaster fasciclin 1, transcript variant A (Fas1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa07932	NM_001275716.1 Latest version!	Drosophila melanogaster fasciclin 1, transcript variant G (Fas1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa07933	NM_001144594.2 Latest version!	Drosophila melanogaster fasciclin 1, transcript variant F (Fas1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa07934	NM_001144593.2 Latest version!	Drosophila melanogaster fasciclin 1, transcript variant E (Fas1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fas1 ( NM_001104344.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097814.1 Drosophila melanogaster fasciclin 1, transcript variant D (Fas1), mRNA.

Price & Availability↑

CloneID	OFa05150
Clone ID Related Accession (Same CDS sequence)	NM_001104344.2
Accession Version	NM_001104344.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fasciclin 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104344.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAACG CTGCAGCGCT GCTTTTGGCG CTGCTCTGCG CCGCGAACGC AGCCGCCGCC 
GCCGATTTGG CGGACAAATT GCGCGATGAT TCGGAACTCT CTCAGAGCAC TGCCAAATCC 
TTGAGGCGCC TTATGCCAGT TAGGTTCTAC AGCCTGCTGG AGAGCAATCA AATTGCCAAC 
TCAACGCTTT CGCTGCGCAG CTGCACGATC TTTGTGCCCA CCAATGAAGC CTTCCAGCGC 
TACAAGAGCA AAACCGCCCA TGTGCTCTAT CACATTACCA CTGAGGCGTA CACCCAGAAA 
CGACTGCCGA ATACCGTGTC ATCGGACATG GCCGGCAATC CACCGCTGTA CATCACAAAG 
AACTCGAATG GCGACATCTT TGTGAACAAT GCCCGGATCA TACCCTCGCT CAGTGTGGAG 
ACAAACAGCG ATGGCAAGCG GCAGATCATG CACATCATCG ACGAGGTACT GGAGCCGCTC 
ACCGTCAAGG CTGGCCATTC GGATACCCCC AACAATCCGA ATGCTCTCAA GTTCCTGAAG 
AACGCCGAGG AGTTCAACGT GGACAACATC GGTGTGCGCA CGTACCGCAG CCAGGTGACG 
ATGGCCAAGA AAGAGTCGGT CTATGATGCC GCCGGACAGC ACACGTTCCT GGTTCCCGTC 
GATGAAGGCT TCAAGCTCTC GGCTCGCAGC AGCCTCGTGG ACGGCAAGGT CATCGATGGC 
CATGTGATAC CAAACACTGT CATCTTCACT GCCGCTGCCC AGCATGACGA TCCCAAGGCT 
TCCGCCGCTT TTGAGGACTT ACTCAAGGTC ACCGTCAGTT TCTTCAAGCA GAAGAACGGC 
AAAATGTACG TCAAGTCAAA CACCATTGTG GGTGATGCCA AACACCGCGT GGGCGTGGTT 
CTGGCCGAAA TCGTGAAGGC GAACATCCCA GTGAGCAACG GAGTAGTCCA TCTGATCCAC 
CGCCCGCTGA TGATCATCGA TACGACGGTC ACCCAATTCC TGCAGGAGAA TGCTGAGAAC 
GGAGCTCTGC GCAAGTTCTA CGAAGTTATA ATGGACAATG GTGGAGCAGT TCTGGACGAC 
ATCAATAGCC TGACAGAAGT GACCATTTTG GCTCCCAGCA ATGAGGCTTG GAACTCCTCG 
AACATCAACA ATGTTTTGCG AGATCGGAAT AAGATGAGGC AGATCCTGAA CATGCATATC 
ATCAAGGACC GCTTAAATGT GGACAAGATC AGGCAGAAAA ATGCAAATTT GATTGCCCAG 
GTGCCCACTG TCAACAACAA CACTTTCCTG TACTTCAACG TTCGCGGTGA GGGATCGGAT 
ACCGTGATAA CAGTTGAGGG AGGCGGCGTG AATGCCACCG TTATCCAGGC TGATGTGGCC 
CAGACTAATG GTTATGTTCA CATCATCGAC CATGTGCTGG GCGTGCCTTA CACTACAGTT 
CTTGGCAAAC TTGAATCCGA TCCCATGATG AGTGACACCT ATAAGATGGG AAAATTCTCG 
CACTTTAATG ACCAGCTGAA CAACACACAA CGCCGCTTCA CCTACTTTGT GCCCAGGGAC 
AAGGGCTGGC AGAAGACCGA GCTGGATTAC CCATCGGCTC ACAAGAAGCT TTTTATGGCC 
GACTTTTCCT ATCATTCCAA GTCCATTCTG GAGCGTCATT TGGCTATTTC GGATAAGGAG 
TACACCATGA AGGATCTGGT TAAGTTTTCG CAAGAATCGG GCAGCGTAAT CCTACCCACG 
TTCCGCGACT CTTTGAGTAT CCGCGTGGAG GAGGAAGCTG GACGCTATGT GATCATTTGG 
AACTACAAGA AGATCAACGT ATACCGGCCC GATGTTGAGT GCACCAACGG AATTATCCAC 
GTCATCGACT ACCCACTCCT GGAGGAAAAG GATGTGGTCG TGGCCGGAGG TAGCTATTTG 
CCAGAATCAA GCATTTGCAT CATCTTGGCC AACCTCATAA TGATAACAGT AGCAAAGTTC 
TTGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097814.1
CDS	249..2237
Translation	MLNAAALLLALLCAANAAAAADLADKLRDDSELSQSTAKSLRRLMPVRFYSLLESNQIANSTLSLRSCTIFVPTNEAFQRYKSKTAHVLYHITTEAYTQKRLPNTVSSDMAGNPPLYITKNSNGDIFVNNARIIPSLSVETNSDGKRQIMHIIDEVLEPLTVKAGHSDTPNNPNALKFLKNAEEFNVDNIGVRTYRSQVTMAKKESVYDAAGQHTFLVPVDEGFKLSARSSLVDGKVIDGHVIPNTVIFTAAAQHDDPKASAAFEDLLKVTVSFFKQKNGKMYVKSNTIVGDAKHRVGVVLAEIVKANIPVSNGVVHLIHRPLMIIDTTVTQFLQENAENGALRKFYEVIMDNGGAVLDDINSLTEVTILAPSNEAWNSSNINNVLRDRNKMRQILNMHIIKDRLNVDKIRQKNANLIAQVPTVNNNTFLYFNVRGEGSDTVITVEGGGVNATVIQADVAQTNGYVHIIDHVLGVPYTTVLGKLESDPMMSDTYKMGKFSHFNDQLNNTQRRFTYFVPRDKGWQKTELDYPSAHKKLFMADFSYHSKSILERHLAISDKEYTMKDLVKFSQESGSVILPTFRDSLSIRVEEEAGRYVIIWNYKKINVYRPDVECTNGIIHVIDYPLLEEKDVVVAGGSYLPESSICIILANLIMITVAKFLN

Target ORF information:

RefSeq Version	NM_001104344.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fasciclin 1, transcript variant D (Fas1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104344.2

ORF Insert Sequence:

ATGCTGAACG CTGCAGCGCT GCTTTTGGCG CTGCTCTGCG CCGCGAACGC AGCCGCCGCC 
GCCGATTTGG CGGACAAATT GCGCGATGAT TCGGAACTCT CTCAGAGCAC TGCCAAATCC 
TTGAGGCGCC TTATGCCAGT TAGGTTCTAC AGCCTGCTGG AGAGCAATCA AATTGCCAAC 
TCAACGCTTT CGCTGCGCAG CTGCACGATC TTTGTGCCCA CCAATGAAGC CTTCCAGCGC 
TACAAGAGCA AAACCGCCCA TGTGCTCTAT CACATTACCA CTGAGGCGTA CACCCAGAAA 
CGACTGCCGA ATACCGTGTC ATCGGACATG GCCGGCAATC CACCGCTGTA CATCACAAAG 
AACTCGAATG GCGACATCTT TGTGAACAAT GCCCGGATCA TACCCTCGCT CAGTGTGGAG 
ACAAACAGCG ATGGCAAGCG GCAGATCATG CACATCATCG ACGAGGTACT GGAGCCGCTC 
ACCGTCAAGG CTGGCCATTC GGATACCCCC AACAATCCGA ATGCTCTCAA GTTCCTGAAG 
AACGCCGAGG AGTTCAACGT GGACAACATC GGTGTGCGCA CGTACCGCAG CCAGGTGACG 
ATGGCCAAGA AAGAGTCGGT CTATGATGCC GCCGGACAGC ACACGTTCCT GGTTCCCGTC 
GATGAAGGCT TCAAGCTCTC GGCTCGCAGC AGCCTCGTGG ACGGCAAGGT CATCGATGGC 
CATGTGATAC CAAACACTGT CATCTTCACT GCCGCTGCCC AGCATGACGA TCCCAAGGCT 
TCCGCCGCTT TTGAGGACTT ACTCAAGGTC ACCGTCAGTT TCTTCAAGCA GAAGAACGGC 
AAAATGTACG TCAAGTCAAA CACCATTGTG GGTGATGCCA AACACCGCGT GGGCGTGGTT 
CTGGCCGAAA TCGTGAAGGC GAACATCCCA GTGAGCAACG GAGTAGTCCA TCTGATCCAC 
CGCCCGCTGA TGATCATCGA TACGACGGTC ACCCAATTCC TGCAGGAGAA TGCTGAGAAC 
GGAGCTCTGC GCAAGTTCTA CGAAGTTATA ATGGACAATG GTGGAGCAGT TCTGGACGAC 
ATCAATAGCC TGACAGAAGT GACCATTTTG GCTCCCAGCA ATGAGGCTTG GAACTCCTCG 
AACATCAACA ATGTTTTGCG AGATCGGAAT AAGATGAGGC AGATCCTGAA CATGCATATC 
ATCAAGGACC GCTTAAATGT GGACAAGATC AGGCAGAAAA ATGCAAATTT GATTGCCCAG 
GTGCCCACTG TCAACAACAA CACTTTCCTG TACTTCAACG TTCGCGGTGA GGGATCGGAT 
ACCGTGATAA CAGTTGAGGG AGGCGGCGTG AATGCCACCG TTATCCAGGC TGATGTGGCC 
CAGACTAATG GTTATGTTCA CATCATCGAC CATGTGCTGG GCGTGCCTTA CACTACAGTT 
CTTGGCAAAC TTGAATCCGA TCCCATGATG AGTGACACCT ATAAGATGGG AAAATTCTCG 
CACTTTAATG ACCAGCTGAA CAACACACAA CGCCGCTTCA CCTACTTTGT GCCCAGGGAC 
AAGGGCTGGC AGAAGACCGA GCTGGATTAC CCATCGGCTC ACAAGAAGCT TTTTATGGCC 
GACTTTTCCT ATCATTCCAA GTCCATTCTG GAGCGTCATT TGGCTATTTC GGATAAGGAG 
TACACCATGA AGGATCTGGT TAAGTTTTCG CAAGAATCGG GCAGCGTAAT CCTACCCACG 
TTCCGCGACT CTTTGAGTAT CCGCGTGGAG GAGGAAGCTG GACGCTATGT GATCATTTGG 
AACTACAAGA AGATCAACGT ATACCGGCCC GATGTTGAGT GCACCAACGG AATTATCCAC 
GTCATCGACT ACCCACTCCT GGAGGAAAAG GATGTGGTCG TGGCCGGAGG TAGCTATTTG 
CCAGAATCAA GCATTTGCAT CATCTTGGCC AACCTCATAA TGATAACAGT AGCAAAGTTC 
TTGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fas1 ( NM_169724.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732164.1 Drosophila melanogaster fasciclin 1, transcript variant C (Fas1), mRNA.

Price & Availability↑

CloneID	OFa05151
Clone ID Related Accession (Same CDS sequence)	NM_169724.2
Accession Version	NM_169724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fasciclin 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169724.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAACG CTGCAGCGCT GCTTTTGGCG CTGCTCTGCG CCGCGAACGC AGCCGCCGCC 
GCCGATTTGG CGGACAAATT GCGCGATGAT TCGGAACTCT CTCAGTTCTA CAGCCTGCTG 
GAGAGCAATC AAATTGCCAA CTCAACGCTT TCGCTGCGCA GCTGCACGAT CTTTGTGCCC 
ACCAATGAAG CCTTCCAGCG CTACAAGAGC AAAACCGCCC ATGTGCTCTA TCACATTACC 
ACTGAGGCGT ACACCCAGAA ACGACTGCCG AATACCGTGT CATCGGACAT GGCCGGCAAT 
CCACCGCTGT ACATCACAAA GAACTCGAAT GGCGACATCT TTGTGAACAA TGCCCGGATC 
ATACCCTCGC TCAGTGTGGA GACAAACAGC GATGGCAAGC GGCAGATCAT GCACATCATC 
GACGAGGTAC TGGAGCCGCT CACCGTCAAG GCTGGCCATT CGGATACCCC CAACAATCCG 
AATGCTCTCA AGTTCCTGAA GAACGCCGAG GAGTTCAACG TGGACAACAT CGGTGTGCGC 
ACGTACCGCA GCCAGGTGAC GATGGCCAAG AAAGAGTCGG TCTATGATGC CGCCGGACAG 
CACACGTTCC TGGTTCCCGT CGATGAAGGC TTCAAGCTCT CGGCTCGCAG CAGCCTCGTG 
GACGGCAAGG TCATCGATGG CCATGTGATA CCAAACACTG TCATCTTCAC TGCCGCTGCC 
CAGCATGACG ATCCCAAGGC TTCCGCCGCT TTTGAGGACT TACTCAAGGT CACCGTCAGT 
TTCTTCAAGC AGAAGAACGG CAAAATGTAC GTCAAGTCAA ACACCATTGT GGGTGATGCC 
AAACACCGCG TGGGCGTGGT TCTGGCCGAA ATCGTGAAGG CGAACATCCC AGTGAGCAAC 
GGAGTAGTCC ATCTGATCCA CCGCCCGCTG ATGATCATCG ATACGACGGT CACCCAATTC 
CTGCAGTCGT TCAAGTTTAT GAACGAGAAT GCTGAGAACG GAGCTCTGCG CAAGTTCTAC 
GAAGTTATAA TGGACAATGG TGGAGCAGTT CTGGACGACA TCAATAGCCT GACAGAAGTG 
ACCATTTTGG CTCCCAGCAA TGAGGCTTGG AACTCCTCGA ACATCAACAA TGTTTTGCGA 
GATCGGAATA AGATGAGGCA GATCCTGAAC ATGCATATCA TCAAGGACCG CTTAAATGTG 
GACAAGATCA GGCAGAAAAA TGCAAATTTG ATTGCCCAGG TGCCCACTGT CAACAACAAC 
ACTTTCCTGT ACTTCAACGT TCGCGGTGAG GGATCGGATA CCGTGATAAC AGTTGAGGGA 
GGCGGCGTGA ATGCCACCGT TATCCAGGCT GATGTGGCCC AGACTAATGG TTATGTTCAC 
ATCATCGACC ATGTGCTGGG CGTGCCTTAC ACTACAGTTC TTGGCAAACT TGAATCCGAT 
CCCATGATGA GTGACACCTA TAAGATGGGA AAATTCTCGC ACTTTAATGA CCAGCTGAAC 
AACACACAAC GCCGCTTCAC CTACTTTGTG CCCAGGGACA AGGGCTGGCA GAAGACCGAG 
CTGGATTACC CATCGGCTCA CAAGAAGCTT TTTATGGCCG ACTTTTCCTA TCATTCCAAG 
TCCATTCTGG AGCGTCATTT GGCTATTTCG GATAAGGAGT ACACCATGAA GGATCTGGTT 
AAGTTTTCGC AAGAATCGGG CAGCGTAATC CTACCCACGT TCCGCGACTC TTTGAGTATC 
CGCGTGGAGG AGGAAGCTGG ACGCTATGTG ATCATTTGGA ACTACAAGAA GATCAACGTA 
TACCGGCCCG ATGTTGAGTG CACCAACGGA ATTATCCACG TCATCGACTA CCCACTCCTG 
GAGGAAAAGG ATGTGGTCGT GGCCGGAGGT AGCTATTTGC CAGAATCAAG CATTTGCATC 
ATCTTGGCCA ACCTCATAAT GATAACAGTA GCAAAGTTCT TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732164.1
CDS	249..2216
Translation	MLNAAALLLALLCAANAAAAADLADKLRDDSELSQFYSLLESNQIANSTLSLRSCTIFVPTNEAFQRYKSKTAHVLYHITTEAYTQKRLPNTVSSDMAGNPPLYITKNSNGDIFVNNARIIPSLSVETNSDGKRQIMHIIDEVLEPLTVKAGHSDTPNNPNALKFLKNAEEFNVDNIGVRTYRSQVTMAKKESVYDAAGQHTFLVPVDEGFKLSARSSLVDGKVIDGHVIPNTVIFTAAAQHDDPKASAAFEDLLKVTVSFFKQKNGKMYVKSNTIVGDAKHRVGVVLAEIVKANIPVSNGVVHLIHRPLMIIDTTVTQFLQSFKFMNENAENGALRKFYEVIMDNGGAVLDDINSLTEVTILAPSNEAWNSSNINNVLRDRNKMRQILNMHIIKDRLNVDKIRQKNANLIAQVPTVNNNTFLYFNVRGEGSDTVITVEGGGVNATVIQADVAQTNGYVHIIDHVLGVPYTTVLGKLESDPMMSDTYKMGKFSHFNDQLNNTQRRFTYFVPRDKGWQKTELDYPSAHKKLFMADFSYHSKSILERHLAISDKEYTMKDLVKFSQESGSVILPTFRDSLSIRVEEEAGRYVIIWNYKKINVYRPDVECTNGIIHVIDYPLLEEKDVVVAGGSYLPESSICIILANLIMITVAKFLN

Target ORF information:

RefSeq Version	NM_169724.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fasciclin 1, transcript variant C (Fas1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169724.2

ORF Insert Sequence:

ATGCTGAACG CTGCAGCGCT GCTTTTGGCG CTGCTCTGCG CCGCGAACGC AGCCGCCGCC 
GCCGATTTGG CGGACAAATT GCGCGATGAT TCGGAACTCT CTCAGTTCTA CAGCCTGCTG 
GAGAGCAATC AAATTGCCAA CTCAACGCTT TCGCTGCGCA GCTGCACGAT CTTTGTGCCC 
ACCAATGAAG CCTTCCAGCG CTACAAGAGC AAAACCGCCC ATGTGCTCTA TCACATTACC 
ACTGAGGCGT ACACCCAGAA ACGACTGCCG AATACCGTGT CATCGGACAT GGCCGGCAAT 
CCACCGCTGT ACATCACAAA GAACTCGAAT GGCGACATCT TTGTGAACAA TGCCCGGATC 
ATACCCTCGC TCAGTGTGGA GACAAACAGC GATGGCAAGC GGCAGATCAT GCACATCATC 
GACGAGGTAC TGGAGCCGCT CACCGTCAAG GCTGGCCATT CGGATACCCC CAACAATCCG 
AATGCTCTCA AGTTCCTGAA GAACGCCGAG GAGTTCAACG TGGACAACAT CGGTGTGCGC 
ACGTACCGCA GCCAGGTGAC GATGGCCAAG AAAGAGTCGG TCTATGATGC CGCCGGACAG 
CACACGTTCC TGGTTCCCGT CGATGAAGGC TTCAAGCTCT CGGCTCGCAG CAGCCTCGTG 
GACGGCAAGG TCATCGATGG CCATGTGATA CCAAACACTG TCATCTTCAC TGCCGCTGCC 
CAGCATGACG ATCCCAAGGC TTCCGCCGCT TTTGAGGACT TACTCAAGGT CACCGTCAGT 
TTCTTCAAGC AGAAGAACGG CAAAATGTAC GTCAAGTCAA ACACCATTGT GGGTGATGCC 
AAACACCGCG TGGGCGTGGT TCTGGCCGAA ATCGTGAAGG CGAACATCCC AGTGAGCAAC 
GGAGTAGTCC ATCTGATCCA CCGCCCGCTG ATGATCATCG ATACGACGGT CACCCAATTC 
CTGCAGTCGT TCAAGTTTAT GAACGAGAAT GCTGAGAACG GAGCTCTGCG CAAGTTCTAC 
GAAGTTATAA TGGACAATGG TGGAGCAGTT CTGGACGACA TCAATAGCCT GACAGAAGTG 
ACCATTTTGG CTCCCAGCAA TGAGGCTTGG AACTCCTCGA ACATCAACAA TGTTTTGCGA 
GATCGGAATA AGATGAGGCA GATCCTGAAC ATGCATATCA TCAAGGACCG CTTAAATGTG 
GACAAGATCA GGCAGAAAAA TGCAAATTTG ATTGCCCAGG TGCCCACTGT CAACAACAAC 
ACTTTCCTGT ACTTCAACGT TCGCGGTGAG GGATCGGATA CCGTGATAAC AGTTGAGGGA 
GGCGGCGTGA ATGCCACCGT TATCCAGGCT GATGTGGCCC AGACTAATGG TTATGTTCAC 
ATCATCGACC ATGTGCTGGG CGTGCCTTAC ACTACAGTTC TTGGCAAACT TGAATCCGAT 
CCCATGATGA GTGACACCTA TAAGATGGGA AAATTCTCGC ACTTTAATGA CCAGCTGAAC 
AACACACAAC GCCGCTTCAC CTACTTTGTG CCCAGGGACA AGGGCTGGCA GAAGACCGAG 
CTGGATTACC CATCGGCTCA CAAGAAGCTT TTTATGGCCG ACTTTTCCTA TCATTCCAAG 
TCCATTCTGG AGCGTCATTT GGCTATTTCG GATAAGGAGT ACACCATGAA GGATCTGGTT 
AAGTTTTCGC AAGAATCGGG CAGCGTAATC CTACCCACGT TCCGCGACTC TTTGAGTATC 
CGCGTGGAGG AGGAAGCTGG ACGCTATGTG ATCATTTGGA ACTACAAGAA GATCAACGTA 
TACCGGCCCG ATGTTGAGTG CACCAACGGA ATTATCCACG TCATCGACTA CCCACTCCTG 
GAGGAAAAGG ATGTGGTCGT GGCCGGAGGT AGCTATTTGC CAGAATCAAG CATTTGCATC 
ATCTTGGCCA ACCTCATAAT GATAACAGTA GCAAAGTTCT TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fas1 ( NM_169725.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732165.1 Drosophila melanogaster fasciclin 1, transcript variant B (Fas1), mRNA.

Price & Availability↑

CloneID	OFa05152
Clone ID Related Accession (Same CDS sequence)	NM_169725.2
Accession Version	NM_169725.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fasciclin 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169725.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAACG CTGCAGCGCT GCTTTTGGCG CTGCTCTGCG CCGCGAACGC AGCCGCCGCC 
GCCGATTTGG CGGACAAATT GCGCGATGAT TCGGAACTCT CTCAGTTCTA CAGCCTGCTG 
GAGAGCAATC AAATTGCCAA CTCAACGCTT TCGCTGCGCA GCTGCACGAT CTTTGTGCCC 
ACCAATGAAG CCTTCCAGCG CTACAAGAGC AAAACCGCCC ATGTGCTCTA TCACATTACC 
ACTGAGGCGT ACACCCAGAA ACGACTGCCG AATACCGTGT CATCGGACAT GGCCGGCAAT 
CCACCGCTGT ACATCACAAA GAACTCGAAT GGCGACATCT TTGTGAACAA TGCCCGGATC 
ATACCCTCGC TCAGTGTGGA GACAAACAGC GATGGCAAGC GGCAGATCAT GCACATCATC 
GACGAGGTAC TGGAGCCGCT CACCGTCAAG GCTGGCCATT CGGATACCCC CAACAATCCG 
AATGCTCTCA AGTTCCTGAA GAACGCCGAG GAGTTCAACG TGGACAACAT CGGTGTGCGC 
ACGTACCGCA GCCAGGTGAC GATGGCCAAG AAAGAGTCGG TCTATGATGC CGCCGGACAG 
CACACGTTCC TGGTTCCCGT CGATGAAGGC TTCAAGCTCT CGGCTCGCAG CAGCCTCGTG 
GACGGCAAGG TCATCGATGG CCATGTGATA CCAAACACTG TCATCTTCAC TGCCGCTGCC 
CAGCATGACG ATCCCAAGGC TTCCGCCGCT TTTGAGGACT TACTCAAGGT CACCGTCAGT 
TTCTTCAAGC AGAAGAACGG CAAAATGTAC GTCAAGTCAA ACACCATTGT GGGTGATGCC 
AAACACCGCG TGGGCGTGGT TCTGGCCGAA ATCGTGAAGG CGAACATCCC AGTGAGCAAC 
GGAGTAGTCC ATCTGATCCA CCGCCCGCTG ATGATCATCG ATACGACGGT CACCCAATTC 
CTGCAGTCGT TCAAGGAGAA TGCTGAGAAC GGAGCTCTGC GCAAGTTCTA CGAAGTTATA 
ATGGACAATG GTGGAGCAGT TCTGGACGAC ATCAATAGCC TGACAGAAGT GACCATTTTG 
GCTCCCAGCA ATGAGGCTTG GAACTCCTCG AACATCAACA ATGTTTTGCG AGATCGGAAT 
AAGATGAGGC AGATCCTGAA CATGCATATC ATCAAGGACC GCTTAAATGT GGACAAGATC 
AGGCAGAAAA ATGCAAATTT GATTGCCCAG GTGCCCACTG TCAACAACAA CACTTTCCTG 
TACTTCAACG TTCGCGGTGA GGGATCGGAT ACCGTGATAA CAGTTGAGGG AGGCGGCGTG 
AATGCCACCG TTATCCAGGC TGATGTGGCC CAGACTAATG GTTATGTTCA CATCATCGAC 
CATGTGCTGG GCGTGCCTTA CACTACAGTT CTTGGCAAAC TTGAATCCGA TCCCATGATG 
AGTGACACCT ATAAGATGGG AAAATTCTCG CACTTTAATG ACCAGCTGAA CAACACACAA 
CGCCGCTTCA CCTACTTTGT GCCCAGGGAC AAGGGCTGGC AGAAGACCGA GCTGGATTAC 
CCATCGGCTC ACAAGAAGCT TTTTATGGCC GACTTTTCCT ATCATTCCAA GTCCATTCTG 
GAGCGTCATT TGGCTATTTC GGATAAGGAG TACACCATGA AGGATCTGGT TAAGTTTTCG 
CAAGAATCGG GCAGCGTAAT CCTACCCACG TTCCGCGACT CTTTGAGTAT CCGCGTGGAG 
GAGGAAGCTG GACGCTATGT GATCATTTGG AACTACAAGA AGATCAACGT ATACCGGCCC 
GATGTTGAGT GCACCAACGG AATTATCCAC GTCATCGACT ACCCACTCCT GGAGGAAAAG 
GATGTGGTCG TGGCCGGAGG TAGCTATTTG CCAGAATCAA GCATTTGCAT CATCTTGGCC 
AACCTCATAA TGATAACAGT AGCAAAGTTC TTGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732165.1
CDS	196..2154
Translation	MLNAAALLLALLCAANAAAAADLADKLRDDSELSQFYSLLESNQIANSTLSLRSCTIFVPTNEAFQRYKSKTAHVLYHITTEAYTQKRLPNTVSSDMAGNPPLYITKNSNGDIFVNNARIIPSLSVETNSDGKRQIMHIIDEVLEPLTVKAGHSDTPNNPNALKFLKNAEEFNVDNIGVRTYRSQVTMAKKESVYDAAGQHTFLVPVDEGFKLSARSSLVDGKVIDGHVIPNTVIFTAAAQHDDPKASAAFEDLLKVTVSFFKQKNGKMYVKSNTIVGDAKHRVGVVLAEIVKANIPVSNGVVHLIHRPLMIIDTTVTQFLQSFKENAENGALRKFYEVIMDNGGAVLDDINSLTEVTILAPSNEAWNSSNINNVLRDRNKMRQILNMHIIKDRLNVDKIRQKNANLIAQVPTVNNNTFLYFNVRGEGSDTVITVEGGGVNATVIQADVAQTNGYVHIIDHVLGVPYTTVLGKLESDPMMSDTYKMGKFSHFNDQLNNTQRRFTYFVPRDKGWQKTELDYPSAHKKLFMADFSYHSKSILERHLAISDKEYTMKDLVKFSQESGSVILPTFRDSLSIRVEEEAGRYVIIWNYKKINVYRPDVECTNGIIHVIDYPLLEEKDVVVAGGSYLPESSICIILANLIMITVAKFLN

Target ORF information:

RefSeq Version	NM_169725.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fasciclin 1, transcript variant B (Fas1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169725.2

ORF Insert Sequence:

ATGCTGAACG CTGCAGCGCT GCTTTTGGCG CTGCTCTGCG CCGCGAACGC AGCCGCCGCC 
GCCGATTTGG CGGACAAATT GCGCGATGAT TCGGAACTCT CTCAGTTCTA CAGCCTGCTG 
GAGAGCAATC AAATTGCCAA CTCAACGCTT TCGCTGCGCA GCTGCACGAT CTTTGTGCCC 
ACCAATGAAG CCTTCCAGCG CTACAAGAGC AAAACCGCCC ATGTGCTCTA TCACATTACC 
ACTGAGGCGT ACACCCAGAA ACGACTGCCG AATACCGTGT CATCGGACAT GGCCGGCAAT 
CCACCGCTGT ACATCACAAA GAACTCGAAT GGCGACATCT TTGTGAACAA TGCCCGGATC 
ATACCCTCGC TCAGTGTGGA GACAAACAGC GATGGCAAGC GGCAGATCAT GCACATCATC 
GACGAGGTAC TGGAGCCGCT CACCGTCAAG GCTGGCCATT CGGATACCCC CAACAATCCG 
AATGCTCTCA AGTTCCTGAA GAACGCCGAG GAGTTCAACG TGGACAACAT CGGTGTGCGC 
ACGTACCGCA GCCAGGTGAC GATGGCCAAG AAAGAGTCGG TCTATGATGC CGCCGGACAG 
CACACGTTCC TGGTTCCCGT CGATGAAGGC TTCAAGCTCT CGGCTCGCAG CAGCCTCGTG 
GACGGCAAGG TCATCGATGG CCATGTGATA CCAAACACTG TCATCTTCAC TGCCGCTGCC 
CAGCATGACG ATCCCAAGGC TTCCGCCGCT TTTGAGGACT TACTCAAGGT CACCGTCAGT 
TTCTTCAAGC AGAAGAACGG CAAAATGTAC GTCAAGTCAA ACACCATTGT GGGTGATGCC 
AAACACCGCG TGGGCGTGGT TCTGGCCGAA ATCGTGAAGG CGAACATCCC AGTGAGCAAC 
GGAGTAGTCC ATCTGATCCA CCGCCCGCTG ATGATCATCG ATACGACGGT CACCCAATTC 
CTGCAGTCGT TCAAGGAGAA TGCTGAGAAC GGAGCTCTGC GCAAGTTCTA CGAAGTTATA 
ATGGACAATG GTGGAGCAGT TCTGGACGAC ATCAATAGCC TGACAGAAGT GACCATTTTG 
GCTCCCAGCA ATGAGGCTTG GAACTCCTCG AACATCAACA ATGTTTTGCG AGATCGGAAT 
AAGATGAGGC AGATCCTGAA CATGCATATC ATCAAGGACC GCTTAAATGT GGACAAGATC 
AGGCAGAAAA ATGCAAATTT GATTGCCCAG GTGCCCACTG TCAACAACAA CACTTTCCTG 
TACTTCAACG TTCGCGGTGA GGGATCGGAT ACCGTGATAA CAGTTGAGGG AGGCGGCGTG 
AATGCCACCG TTATCCAGGC TGATGTGGCC CAGACTAATG GTTATGTTCA CATCATCGAC 
CATGTGCTGG GCGTGCCTTA CACTACAGTT CTTGGCAAAC TTGAATCCGA TCCCATGATG 
AGTGACACCT ATAAGATGGG AAAATTCTCG CACTTTAATG ACCAGCTGAA CAACACACAA 
CGCCGCTTCA CCTACTTTGT GCCCAGGGAC AAGGGCTGGC AGAAGACCGA GCTGGATTAC 
CCATCGGCTC ACAAGAAGCT TTTTATGGCC GACTTTTCCT ATCATTCCAA GTCCATTCTG 
GAGCGTCATT TGGCTATTTC GGATAAGGAG TACACCATGA AGGATCTGGT TAAGTTTTCG 
CAAGAATCGG GCAGCGTAAT CCTACCCACG TTCCGCGACT CTTTGAGTAT CCGCGTGGAG 
GAGGAAGCTG GACGCTATGT GATCATTTGG AACTACAAGA AGATCAACGT ATACCGGCCC 
GATGTTGAGT GCACCAACGG AATTATCCAC GTCATCGACT ACCCACTCCT GGAGGAAAAG 
GATGTGGTCG TGGCCGGAGG TAGCTATTTG CCAGAATCAA GCATTTGCAT CATCTTGGCC 
AACCTCATAA TGATAACAGT AGCAAAGTTC TTGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fas1 ( NM_169726.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732166.1 Drosophila melanogaster fasciclin 1, transcript variant A (Fas1), mRNA.

Price & Availability↑

CloneID	OFa05153
Clone ID Related Accession (Same CDS sequence)	NM_169726.2
Accession Version	NM_169726.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fasciclin 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169726.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAACG CTGCAGCGCT GCTTTTGGCG CTGCTCTGCG CCGCGAACGC AGCCGCCGCC 
GCCGATTTGG CGGACAAATT GCGCGATGAT TCGGAACTCT CTCAGTTCTA CAGCCTGCTG 
GAGAGCAATC AAATTGCCAA CTCAACGCTT TCGCTGCGCA GCTGCACGAT CTTTGTGCCC 
ACCAATGAAG CCTTCCAGCG CTACAAGAGC AAAACCGCCC ATGTGCTCTA TCACATTACC 
ACTGAGGCGT ACACCCAGAA ACGACTGCCG AATACCGTGT CATCGGACAT GGCCGGCAAT 
CCACCGCTGT ACATCACAAA GAACTCGAAT GGCGACATCT TTGTGAACAA TGCCCGGATC 
ATACCCTCGC TCAGTGTGGA GACAAACAGC GATGGCAAGC GGCAGATCAT GCACATCATC 
GACGAGGTAC TGGAGCCGCT CACCGTCAAG GCTGGCCATT CGGATACCCC CAACAATCCG 
AATGCTCTCA AGTTCCTGAA GAACGCCGAG GAGTTCAACG TGGACAACAT CGGTGTGCGC 
ACGTACCGCA GCCAGGTGAC GATGGCCAAG AAAGAGTCGG TCTATGATGC CGCCGGACAG 
CACACGTTCC TGGTTCCCGT CGATGAAGGC TTCAAGCTCT CGGCTCGCAG CAGCCTCGTG 
GACGGCAAGG TCATCGATGG CCATGTGATA CCAAACACTG TCATCTTCAC TGCCGCTGCC 
CAGCATGACG ATCCCAAGGC TTCCGCCGCT TTTGAGGACT TACTCAAGGT CACCGTCAGT 
TTCTTCAAGC AGAAGAACGG CAAAATGTAC GTCAAGTCAA ACACCATTGT GGGTGATGCC 
AAACACCGCG TGGGCGTGGT TCTGGCCGAA ATCGTGAAGG CGAACATCCC AGTGAGCAAC 
GGAGTAGTCC ATCTGATCCA CCGCCCGCTG ATGATCATCG ATACGACGGT CACCCAATTC 
CTGCAGGAGA ATGCTGAGAA CGGAGCTCTG CGCAAGTTCT ACGAAGTTAT AATGGACAAT 
GGTGGAGCAG TTCTGGACGA CATCAATAGC CTGACAGAAG TGACCATTTT GGCTCCCAGC 
AATGAGGCTT GGAACTCCTC GAACATCAAC AATGTTTTGC GAGATCGGAA TAAGATGAGG 
CAGATCCTGA ACATGCATAT CATCAAGGAC CGCTTAAATG TGGACAAGAT CAGGCAGAAA 
AATGCAAATT TGATTGCCCA GGTGCCCACT GTCAACAACA ACACTTTCCT GTACTTCAAC 
GTTCGCGGTG AGGGATCGGA TACCGTGATA ACAGTTGAGG GAGGCGGCGT GAATGCCACC 
GTTATCCAGG CTGATGTGGC CCAGACTAAT GGTTATGTTC ACATCATCGA CCATGTGCTG 
GGCGTGCCTT ACACTACAGT TCTTGGCAAA CTTGAATCCG ATCCCATGAT GAGTGACACC 
TATAAGATGG GAAAATTCTC GCACTTTAAT GACCAGCTGA ACAACACACA ACGCCGCTTC 
ACCTACTTTG TGCCCAGGGA CAAGGGCTGG CAGAAGACCG AGCTGGATTA CCCATCGGCT 
CACAAGAAGC TTTTTATGGC CGACTTTTCC TATCATTCCA AGTCCATTCT GGAGCGTCAT 
TTGGCTATTT CGGATAAGGA GTACACCATG AAGGATCTGG TTAAGTTTTC GCAAGAATCG 
GGCAGCGTAA TCCTACCCAC GTTCCGCGAC TCTTTGAGTA TCCGCGTGGA GGAGGAAGCT 
GGACGCTATG TGATCATTTG GAACTACAAG AAGATCAACG TATACCGGCC CGATGTTGAG 
TGCACCAACG GAATTATCCA CGTCATCGAC TACCCACTCC TGGAGGAAAA GGATGTGGTC 
GTGGCCGGAG GTAGCTATTT GCCAGAATCA AGCATTTGCA TCATCTTGGC CAACCTCATA 
ATGATAACAG TAGCAAAGTT CTTGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732166.1
CDS	196..2145
Translation	MLNAAALLLALLCAANAAAAADLADKLRDDSELSQFYSLLESNQIANSTLSLRSCTIFVPTNEAFQRYKSKTAHVLYHITTEAYTQKRLPNTVSSDMAGNPPLYITKNSNGDIFVNNARIIPSLSVETNSDGKRQIMHIIDEVLEPLTVKAGHSDTPNNPNALKFLKNAEEFNVDNIGVRTYRSQVTMAKKESVYDAAGQHTFLVPVDEGFKLSARSSLVDGKVIDGHVIPNTVIFTAAAQHDDPKASAAFEDLLKVTVSFFKQKNGKMYVKSNTIVGDAKHRVGVVLAEIVKANIPVSNGVVHLIHRPLMIIDTTVTQFLQENAENGALRKFYEVIMDNGGAVLDDINSLTEVTILAPSNEAWNSSNINNVLRDRNKMRQILNMHIIKDRLNVDKIRQKNANLIAQVPTVNNNTFLYFNVRGEGSDTVITVEGGGVNATVIQADVAQTNGYVHIIDHVLGVPYTTVLGKLESDPMMSDTYKMGKFSHFNDQLNNTQRRFTYFVPRDKGWQKTELDYPSAHKKLFMADFSYHSKSILERHLAISDKEYTMKDLVKFSQESGSVILPTFRDSLSIRVEEEAGRYVIIWNYKKINVYRPDVECTNGIIHVIDYPLLEEKDVVVAGGSYLPESSICIILANLIMITVAKFLN

Target ORF information:

RefSeq Version	NM_169726.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fasciclin 1, transcript variant A (Fas1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169726.2

ORF Insert Sequence:

ATGCTGAACG CTGCAGCGCT GCTTTTGGCG CTGCTCTGCG CCGCGAACGC AGCCGCCGCC 
GCCGATTTGG CGGACAAATT GCGCGATGAT TCGGAACTCT CTCAGTTCTA CAGCCTGCTG 
GAGAGCAATC AAATTGCCAA CTCAACGCTT TCGCTGCGCA GCTGCACGAT CTTTGTGCCC 
ACCAATGAAG CCTTCCAGCG CTACAAGAGC AAAACCGCCC ATGTGCTCTA TCACATTACC 
ACTGAGGCGT ACACCCAGAA ACGACTGCCG AATACCGTGT CATCGGACAT GGCCGGCAAT 
CCACCGCTGT ACATCACAAA GAACTCGAAT GGCGACATCT TTGTGAACAA TGCCCGGATC 
ATACCCTCGC TCAGTGTGGA GACAAACAGC GATGGCAAGC GGCAGATCAT GCACATCATC 
GACGAGGTAC TGGAGCCGCT CACCGTCAAG GCTGGCCATT CGGATACCCC CAACAATCCG 
AATGCTCTCA AGTTCCTGAA GAACGCCGAG GAGTTCAACG TGGACAACAT CGGTGTGCGC 
ACGTACCGCA GCCAGGTGAC GATGGCCAAG AAAGAGTCGG TCTATGATGC CGCCGGACAG 
CACACGTTCC TGGTTCCCGT CGATGAAGGC TTCAAGCTCT CGGCTCGCAG CAGCCTCGTG 
GACGGCAAGG TCATCGATGG CCATGTGATA CCAAACACTG TCATCTTCAC TGCCGCTGCC 
CAGCATGACG ATCCCAAGGC TTCCGCCGCT TTTGAGGACT TACTCAAGGT CACCGTCAGT 
TTCTTCAAGC AGAAGAACGG CAAAATGTAC GTCAAGTCAA ACACCATTGT GGGTGATGCC 
AAACACCGCG TGGGCGTGGT TCTGGCCGAA ATCGTGAAGG CGAACATCCC AGTGAGCAAC 
GGAGTAGTCC ATCTGATCCA CCGCCCGCTG ATGATCATCG ATACGACGGT CACCCAATTC 
CTGCAGGAGA ATGCTGAGAA CGGAGCTCTG CGCAAGTTCT ACGAAGTTAT AATGGACAAT 
GGTGGAGCAG TTCTGGACGA CATCAATAGC CTGACAGAAG TGACCATTTT GGCTCCCAGC 
AATGAGGCTT GGAACTCCTC GAACATCAAC AATGTTTTGC GAGATCGGAA TAAGATGAGG 
CAGATCCTGA ACATGCATAT CATCAAGGAC CGCTTAAATG TGGACAAGAT CAGGCAGAAA 
AATGCAAATT TGATTGCCCA GGTGCCCACT GTCAACAACA ACACTTTCCT GTACTTCAAC 
GTTCGCGGTG AGGGATCGGA TACCGTGATA ACAGTTGAGG GAGGCGGCGT GAATGCCACC 
GTTATCCAGG CTGATGTGGC CCAGACTAAT GGTTATGTTC ACATCATCGA CCATGTGCTG 
GGCGTGCCTT ACACTACAGT TCTTGGCAAA CTTGAATCCG ATCCCATGAT GAGTGACACC 
TATAAGATGG GAAAATTCTC GCACTTTAAT GACCAGCTGA ACAACACACA ACGCCGCTTC 
ACCTACTTTG TGCCCAGGGA CAAGGGCTGG CAGAAGACCG AGCTGGATTA CCCATCGGCT 
CACAAGAAGC TTTTTATGGC CGACTTTTCC TATCATTCCA AGTCCATTCT GGAGCGTCAT 
TTGGCTATTT CGGATAAGGA GTACACCATG AAGGATCTGG TTAAGTTTTC GCAAGAATCG 
GGCAGCGTAA TCCTACCCAC GTTCCGCGAC TCTTTGAGTA TCCGCGTGGA GGAGGAAGCT 
GGACGCTATG TGATCATTTG GAACTACAAG AAGATCAACG TATACCGGCC CGATGTTGAG 
TGCACCAACG GAATTATCCA CGTCATCGAC TACCCACTCC TGGAGGAAAA GGATGTGGTC 
GTGGCCGGAG GTAGCTATTT GCCAGAATCA AGCATTTGCA TCATCTTGGC CAACCTCATA 
ATGATAACAG TAGCAAAGTT CTTGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fas1 ( NM_001275716.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262645.1 Drosophila melanogaster fasciclin 1, transcript variant G (Fas1), mRNA.

Price & Availability↑

CloneID	OFa07932
Clone ID Related Accession (Same CDS sequence)	NM_001275716.1
Accession Version	NM_001275716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fasciclin 1, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAACG CTGCAGCGCT GCTTTTGGCG CTGCTCTGCG CCGCGAACGC AGCCGCCGCC 
GCCGATTTGG CGGACAAATT GCGCGATGAT TCGGAACTCT CTCAGTTCTA CAGCCTGCTG 
GAGAGCAATC AAATTGCCAA CTCAACGCTT TCGCTGCGCA GCTGCACGAT CTTTGTGCCC 
ACCAATGAAG CCTTCCAGCG CTACAAGAGC AAAACCGCCC ATGTGCTCTA TCACATTACC 
ACTGAGGCGT ACACCCAGAA ACGACTGCCG AATACCGTGT CATCGGACAT GGCCGGCAAT 
CCACCGCTGT ACATCACAAA GAACTCGAAT GGCGACATCT TTGTGAACAA TGCCCGGATC 
ATACCCTCGC TCAGTGTGGA GACAAACAGC GATGGCAAGC GGCAGATCAT GCACATCATC 
GACGAGGTAC TGGAGCCGCT CACCGTCAAG GCTGGCCATT CGGATACCCC CAACAATCCG 
AATGCTCTCA AGTTCCTGAA GAACGCCGAG GAGTTCAACG TGGACAACAT CGGTGTGCGC 
ACGTACCGCA GCCAGGTGAC GATGGCCAAG AAAGAGTCGG TCTATGATGC CGCCGGACAG 
CACACGTTCC TGGTTCCCGT CGATGAAGGC TTCAAGCTCT CGGCTCGCAG CAGCCTCGTG 
GACGGCAAGG TCATCGATGG CCATGTGATA CCAAACACTG TCATCTTCAC TGCCGCTGCC 
CAGCATGACG ATCCCAAGGC TTCCGCCGCT TTTGAGGACT TACTCAAGGT CACCGTCAGT 
TTCTTCAAGC AGAAGAACGG CAAAATGTAC GTCAAGTCAA ACACCATTGT GGGTGATGCC 
AAACACCGCG TGGGCGTGGT TCTGGCCGAA ATCGTGAAGG CGAACATCCC AGTGAGCAAC 
GGAGTAGTCC ATCTGATCCA CCGCCCGCTG ATGATCATCG ATACGACGGT CACCCAATTC 
CTGCAGGAGA ATGCTGAGAA CGGAGCTCTG CGCAAGTTCT ACGAAGTTAT AATGGACAAT 
GGTGGAGCAG TTCTGGACGA CATCAATAGC CTGACAGAAG TGACCATTTT GGCTCCCAGC 
AATGAGGCTT GGAACTCCTC GAACATCAAC AATGTTTTGC GAGATCGGAA TAAGATGAGG 
CAGATCCTGA ACATGCATAT CATCAAGGAC CGCTTAAATG TGGACAAGAT CAGGCAGAAA 
AATGCAAATT TGATTGCCCA GGTGCCCACT GTCAACAACA ACACTTTCCT GTACTTCAAC 
GTTCGCGGTG AGGGATCGGA TACCGTGATA ACAGTTGAGG GAGGCGGCGT GAATGCCACC 
GTTATCCAGG CTGATGTGGC CCAGACTAAT GGTTATGTTC ACATCATCGA CCATGTGCTG 
GGCGTGCCTT ACACTACAGT TCTTGGCAAA CTTGAATCCG ATCCCATGAT GAGTGACACC 
TATAAGATGG GAAAATTCTC GCACTTTAAT GACCAGCTGA ACAACACACA ACGCCGCTTC 
ACCTACTTTG TGCCCAGGGA CAAGGGCTGG CAGAAGACCG AGCTGGATTA CCCATCGGCT 
CACAAGAAGC TTTTTATGGC CGACTTTTCC TATCATTCCA AGTCCATTCT GGAGCGTCAT 
TTGGCTATTT CGGATAAGGA GTACACCATG AAGGATCTGG TTAAGTTTTC GCAAGAATCG 
GGCAGCGTAA TCCTACCCAC GTTCCGCGAC TCTTTGAGTA TCCGCGTGGA GGAGGAAGCT 
GGACATCTCC ATGATGAGTA TGCTAGTCAC GAATGGACTG GCTATGTGAT CATTTGGAAC 
TACAAGAAGA TCAACGTATA CCGGCCCGAT GTTGAGTGCA CCAACGGAAT TATCCACGTC 
ATCGACTACC CACTCCTGGA GGAAAAGGAT GTGGTCGTGG CCGGAGGTAG CTATTTGCCA 
GAATCAAGCA TTTGCATCAT CTTGGCCAAC CTCATAATGA TAACAGTAGC AAAGTTCTTG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262645.1
CDS	249..2234
Translation	MLNAAALLLALLCAANAAAAADLADKLRDDSELSQFYSLLESNQIANSTLSLRSCTIFVPTNEAFQRYKSKTAHVLYHITTEAYTQKRLPNTVSSDMAGNPPLYITKNSNGDIFVNNARIIPSLSVETNSDGKRQIMHIIDEVLEPLTVKAGHSDTPNNPNALKFLKNAEEFNVDNIGVRTYRSQVTMAKKESVYDAAGQHTFLVPVDEGFKLSARSSLVDGKVIDGHVIPNTVIFTAAAQHDDPKASAAFEDLLKVTVSFFKQKNGKMYVKSNTIVGDAKHRVGVVLAEIVKANIPVSNGVVHLIHRPLMIIDTTVTQFLQENAENGALRKFYEVIMDNGGAVLDDINSLTEVTILAPSNEAWNSSNINNVLRDRNKMRQILNMHIIKDRLNVDKIRQKNANLIAQVPTVNNNTFLYFNVRGEGSDTVITVEGGGVNATVIQADVAQTNGYVHIIDHVLGVPYTTVLGKLESDPMMSDTYKMGKFSHFNDQLNNTQRRFTYFVPRDKGWQKTELDYPSAHKKLFMADFSYHSKSILERHLAISDKEYTMKDLVKFSQESGSVILPTFRDSLSIRVEEEAGHLHDEYASHEWTGYVIIWNYKKINVYRPDVECTNGIIHVIDYPLLEEKDVVVAGGSYLPESSICIILANLIMITVAKFLN

Target ORF information:

RefSeq Version	NM_001275716.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fasciclin 1, transcript variant G (Fas1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275716.1

ORF Insert Sequence:

ATGCTGAACG CTGCAGCGCT GCTTTTGGCG CTGCTCTGCG CCGCGAACGC AGCCGCCGCC 
GCCGATTTGG CGGACAAATT GCGCGATGAT TCGGAACTCT CTCAGTTCTA CAGCCTGCTG 
GAGAGCAATC AAATTGCCAA CTCAACGCTT TCGCTGCGCA GCTGCACGAT CTTTGTGCCC 
ACCAATGAAG CCTTCCAGCG CTACAAGAGC AAAACCGCCC ATGTGCTCTA TCACATTACC 
ACTGAGGCGT ACACCCAGAA ACGACTGCCG AATACCGTGT CATCGGACAT GGCCGGCAAT 
CCACCGCTGT ACATCACAAA GAACTCGAAT GGCGACATCT TTGTGAACAA TGCCCGGATC 
ATACCCTCGC TCAGTGTGGA GACAAACAGC GATGGCAAGC GGCAGATCAT GCACATCATC 
GACGAGGTAC TGGAGCCGCT CACCGTCAAG GCTGGCCATT CGGATACCCC CAACAATCCG 
AATGCTCTCA AGTTCCTGAA GAACGCCGAG GAGTTCAACG TGGACAACAT CGGTGTGCGC 
ACGTACCGCA GCCAGGTGAC GATGGCCAAG AAAGAGTCGG TCTATGATGC CGCCGGACAG 
CACACGTTCC TGGTTCCCGT CGATGAAGGC TTCAAGCTCT CGGCTCGCAG CAGCCTCGTG 
GACGGCAAGG TCATCGATGG CCATGTGATA CCAAACACTG TCATCTTCAC TGCCGCTGCC 
CAGCATGACG ATCCCAAGGC TTCCGCCGCT TTTGAGGACT TACTCAAGGT CACCGTCAGT 
TTCTTCAAGC AGAAGAACGG CAAAATGTAC GTCAAGTCAA ACACCATTGT GGGTGATGCC 
AAACACCGCG TGGGCGTGGT TCTGGCCGAA ATCGTGAAGG CGAACATCCC AGTGAGCAAC 
GGAGTAGTCC ATCTGATCCA CCGCCCGCTG ATGATCATCG ATACGACGGT CACCCAATTC 
CTGCAGGAGA ATGCTGAGAA CGGAGCTCTG CGCAAGTTCT ACGAAGTTAT AATGGACAAT 
GGTGGAGCAG TTCTGGACGA CATCAATAGC CTGACAGAAG TGACCATTTT GGCTCCCAGC 
AATGAGGCTT GGAACTCCTC GAACATCAAC AATGTTTTGC GAGATCGGAA TAAGATGAGG 
CAGATCCTGA ACATGCATAT CATCAAGGAC CGCTTAAATG TGGACAAGAT CAGGCAGAAA 
AATGCAAATT TGATTGCCCA GGTGCCCACT GTCAACAACA ACACTTTCCT GTACTTCAAC 
GTTCGCGGTG AGGGATCGGA TACCGTGATA ACAGTTGAGG GAGGCGGCGT GAATGCCACC 
GTTATCCAGG CTGATGTGGC CCAGACTAAT GGTTATGTTC ACATCATCGA CCATGTGCTG 
GGCGTGCCTT ACACTACAGT TCTTGGCAAA CTTGAATCCG ATCCCATGAT GAGTGACACC 
TATAAGATGG GAAAATTCTC GCACTTTAAT GACCAGCTGA ACAACACACA ACGCCGCTTC 
ACCTACTTTG TGCCCAGGGA CAAGGGCTGG CAGAAGACCG AGCTGGATTA CCCATCGGCT 
CACAAGAAGC TTTTTATGGC CGACTTTTCC TATCATTCCA AGTCCATTCT GGAGCGTCAT 
TTGGCTATTT CGGATAAGGA GTACACCATG AAGGATCTGG TTAAGTTTTC GCAAGAATCG 
GGCAGCGTAA TCCTACCCAC GTTCCGCGAC TCTTTGAGTA TCCGCGTGGA GGAGGAAGCT 
GGACATCTCC ATGATGAGTA TGCTAGTCAC GAATGGACTG GCTATGTGAT CATTTGGAAC 
TACAAGAAGA TCAACGTATA CCGGCCCGAT GTTGAGTGCA CCAACGGAAT TATCCACGTC 
ATCGACTACC CACTCCTGGA GGAAAAGGAT GTGGTCGTGG CCGGAGGTAG CTATTTGCCA 
GAATCAAGCA TTTGCATCAT CTTGGCCAAC CTCATAATGA TAACAGTAGC AAAGTTCTTG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fas1 ( NM_001144594.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138066.1 Drosophila melanogaster fasciclin 1, transcript variant F (Fas1), mRNA.

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CloneID	OFa07933
Clone ID Related Accession (Same CDS sequence)	NM_001144594.2
Accession Version	NM_001144594.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fasciclin 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001144594.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAACG CTGCAGCGCT GCTTTTGGCG CTGCTCTGCG CCGCGAACGC AGCCGCCGCC 
GCCGATTTGG CGGACAAATT GCGCGATGAT TCGGAACTCT CTCAGTTCTA CAGCCTGCTG 
GAGAGCAATC AAATTGCCAA CTCAACGCTT TCGCTGCGCA GCTGCACGAT CTTTGTGCCC 
ACCAATGAAG CCTTCCAGCG CTACAAGAGC AAAACCGCCC ATGTGCTCTA TCACATTACC 
ACTGAGGCGT ACACCCAGAA ACGACTGCCG AATACCGTGT CATCGGACAT GGCCGGCAAT 
CCACCGCTGT ACATCACAAA GAACTCGAAT GGCGACATCT TTGTGAACAA TGCCCGGATC 
ATACCCTCGC TCAGTGTGGA GACAAACAGC GATGGCAAGC GGCAGATCAT GCACATCATC 
GACGAGGTAC TGGAGCCGCT CACCGTCAAG GCTGGCCATT CGGATACCCC CAACAATCCG 
AATGCTCTCA AGTTCCTGAA GAACGCCGAG GAGTTCAACG TGGACAACAT CGGTGTGCGC 
ACGTACCGCA GCCAGGTGAC GATGGCCAAG AAAGAGTCGG TCTATGATGC CGCCGGACAG 
CACACGTTCC TGGTTCCCGT CGATGAAGGC TTCAAGCTCT CGGCTCGCAG CAGCCTCGTG 
GACGGCAAGG TCATCGATGG CCATGTGATA CCAAACACTG TCATCTTCAC TGCCGCTGCC 
CAGCATGACG ATCCCAAGGC TTCCGCCGCT TTTGAGGACT TACTCAAGGT CACCGTCAGT 
TTCTTCAAGC AGAAGAACGG CAAAATGTAC GTCAAGTCAA ACACCATTGT GGGTGATGCC 
AAACACCGCG TGGGCGTGGT TCTGGCCGAA ATCGTGAAGG CGAACATCCC AGTGAGCAAC 
GGAGTAGTCC ATCTGATCCA CCGCCCGCTG ATGATCATCG ATACGACGGT CACCCAATTC 
CTGCAGGAGA ATGCTGAGAA CGGAGCTCTG CGCAAGTTCT ACGAAGTTAT AATGGACAAT 
GGTGGAGCAG TTCTGGACGA CATCAATAGC CTGACAGAAG TGACCATTTT GGCTCCCAGC 
AATGAGGCTT GGAACTCCTC GAACATCAAC AATGTTTTGC GAGATCGGAA TAAGATGAGG 
CAGATCCTGA ACATGCATAT CATCAAGGAC CGCTTAAATG TGGACAAGAT CAGGCAGAAA 
AATGCAAATT TGATTGCCCA GGTGCCCACT GTCAACAACA ACACTTTCCT GTACTTCAAC 
GTTCGCGGTG AGGGATCGGA TACCGTGATA ACAGTTGAGG GAGGCGGCGT GAATGCCACC 
GTTATCCAGG CTGATGTGGC CCAGACTAAT GGTTATGTTC ACATCATCGA CCATGTGCTG 
GGCGTGCCTT ACACTACAGT TCTTGGCAAA CTTGAATCCG ATCCCATGAT GAGTGACACC 
TATAAGATGG GAAAATTCTC GCACTTTAAT GACCAGCTGA ACAACACACA ACGCCGCTTC 
ACCTACTTTG TGCCCAGGGA CAAGGGCTGG CAGAAGACCG AGCTGGATTA CCCATCGGCT 
CACAAGAAGC TTTTTATGGC CGACTTTTCC TATCATTCCA AGTCCATTCT GGAGCGTCAT 
TTGGCTATTT CGGATAAGGA GTACACCATG AAGGATCTGG TTAAGTTTTC GCAAGAATCG 
GGCAGCGTAA TCCTACCCAC GTTCCGCGAC TCTTTGAGTA TCCGCGTGGA GGAGGAAGCT 
GGACATCTCC ATGATGAGTA TGCTAGTCAC GAATGGACTG GCTATGTGAT CATTTGGAAC 
TACAAGAAGA TCAACGTATA CCGGCCCGAT GTTGAGTGCA CCAACGGAAT TATCCACGTC 
ATCGACTACC CACTCCTGGA GGAAAAGGAT GTGGTCGTGG CCGGAGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138066.1
CDS	249..2159
Translation	MLNAAALLLALLCAANAAAAADLADKLRDDSELSQFYSLLESNQIANSTLSLRSCTIFVPTNEAFQRYKSKTAHVLYHITTEAYTQKRLPNTVSSDMAGNPPLYITKNSNGDIFVNNARIIPSLSVETNSDGKRQIMHIIDEVLEPLTVKAGHSDTPNNPNALKFLKNAEEFNVDNIGVRTYRSQVTMAKKESVYDAAGQHTFLVPVDEGFKLSARSSLVDGKVIDGHVIPNTVIFTAAAQHDDPKASAAFEDLLKVTVSFFKQKNGKMYVKSNTIVGDAKHRVGVVLAEIVKANIPVSNGVVHLIHRPLMIIDTTVTQFLQENAENGALRKFYEVIMDNGGAVLDDINSLTEVTILAPSNEAWNSSNINNVLRDRNKMRQILNMHIIKDRLNVDKIRQKNANLIAQVPTVNNNTFLYFNVRGEGSDTVITVEGGGVNATVIQADVAQTNGYVHIIDHVLGVPYTTVLGKLESDPMMSDTYKMGKFSHFNDQLNNTQRRFTYFVPRDKGWQKTELDYPSAHKKLFMADFSYHSKSILERHLAISDKEYTMKDLVKFSQESGSVILPTFRDSLSIRVEEEAGHLHDEYASHEWTGYVIIWNYKKINVYRPDVECTNGIIHVIDYPLLEEKDVVVAGG

Target ORF information:

RefSeq Version	NM_001144594.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fasciclin 1, transcript variant F (Fas1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144594.2

ORF Insert Sequence:

ATGCTGAACG CTGCAGCGCT GCTTTTGGCG CTGCTCTGCG CCGCGAACGC AGCCGCCGCC 
GCCGATTTGG CGGACAAATT GCGCGATGAT TCGGAACTCT CTCAGTTCTA CAGCCTGCTG 
GAGAGCAATC AAATTGCCAA CTCAACGCTT TCGCTGCGCA GCTGCACGAT CTTTGTGCCC 
ACCAATGAAG CCTTCCAGCG CTACAAGAGC AAAACCGCCC ATGTGCTCTA TCACATTACC 
ACTGAGGCGT ACACCCAGAA ACGACTGCCG AATACCGTGT CATCGGACAT GGCCGGCAAT 
CCACCGCTGT ACATCACAAA GAACTCGAAT GGCGACATCT TTGTGAACAA TGCCCGGATC 
ATACCCTCGC TCAGTGTGGA GACAAACAGC GATGGCAAGC GGCAGATCAT GCACATCATC 
GACGAGGTAC TGGAGCCGCT CACCGTCAAG GCTGGCCATT CGGATACCCC CAACAATCCG 
AATGCTCTCA AGTTCCTGAA GAACGCCGAG GAGTTCAACG TGGACAACAT CGGTGTGCGC 
ACGTACCGCA GCCAGGTGAC GATGGCCAAG AAAGAGTCGG TCTATGATGC CGCCGGACAG 
CACACGTTCC TGGTTCCCGT CGATGAAGGC TTCAAGCTCT CGGCTCGCAG CAGCCTCGTG 
GACGGCAAGG TCATCGATGG CCATGTGATA CCAAACACTG TCATCTTCAC TGCCGCTGCC 
CAGCATGACG ATCCCAAGGC TTCCGCCGCT TTTGAGGACT TACTCAAGGT CACCGTCAGT 
TTCTTCAAGC AGAAGAACGG CAAAATGTAC GTCAAGTCAA ACACCATTGT GGGTGATGCC 
AAACACCGCG TGGGCGTGGT TCTGGCCGAA ATCGTGAAGG CGAACATCCC AGTGAGCAAC 
GGAGTAGTCC ATCTGATCCA CCGCCCGCTG ATGATCATCG ATACGACGGT CACCCAATTC 
CTGCAGGAGA ATGCTGAGAA CGGAGCTCTG CGCAAGTTCT ACGAAGTTAT AATGGACAAT 
GGTGGAGCAG TTCTGGACGA CATCAATAGC CTGACAGAAG TGACCATTTT GGCTCCCAGC 
AATGAGGCTT GGAACTCCTC GAACATCAAC AATGTTTTGC GAGATCGGAA TAAGATGAGG 
CAGATCCTGA ACATGCATAT CATCAAGGAC CGCTTAAATG TGGACAAGAT CAGGCAGAAA 
AATGCAAATT TGATTGCCCA GGTGCCCACT GTCAACAACA ACACTTTCCT GTACTTCAAC 
GTTCGCGGTG AGGGATCGGA TACCGTGATA ACAGTTGAGG GAGGCGGCGT GAATGCCACC 
GTTATCCAGG CTGATGTGGC CCAGACTAAT GGTTATGTTC ACATCATCGA CCATGTGCTG 
GGCGTGCCTT ACACTACAGT TCTTGGCAAA CTTGAATCCG ATCCCATGAT GAGTGACACC 
TATAAGATGG GAAAATTCTC GCACTTTAAT GACCAGCTGA ACAACACACA ACGCCGCTTC 
ACCTACTTTG TGCCCAGGGA CAAGGGCTGG CAGAAGACCG AGCTGGATTA CCCATCGGCT 
CACAAGAAGC TTTTTATGGC CGACTTTTCC TATCATTCCA AGTCCATTCT GGAGCGTCAT 
TTGGCTATTT CGGATAAGGA GTACACCATG AAGGATCTGG TTAAGTTTTC GCAAGAATCG 
GGCAGCGTAA TCCTACCCAC GTTCCGCGAC TCTTTGAGTA TCCGCGTGGA GGAGGAAGCT 
GGACATCTCC ATGATGAGTA TGCTAGTCAC GAATGGACTG GCTATGTGAT CATTTGGAAC 
TACAAGAAGA TCAACGTATA CCGGCCCGAT GTTGAGTGCA CCAACGGAAT TATCCACGTC 
ATCGACTACC CACTCCTGGA GGAAAAGGAT GTGGTCGTGG CCGGAGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fas1 ( NM_001144593.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138065.1 Drosophila melanogaster fasciclin 1, transcript variant E (Fas1), mRNA.

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CloneID	OFa07934
Clone ID Related Accession (Same CDS sequence)	NM_001144593.2
Accession Version	NM_001144593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fasciclin 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001144593.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAACG CTGCAGCGCT GCTTTTGGCG CTGCTCTGCG CCGCGAACGC AGCCGCCGCC 
GCCGATTTGG CGGACAAATT GCGCGATGAT TCGGAACTCT CTCAGTTCTA CAGCCTGCTG 
GAGAGCAATC AAATTGCCAA CTCAACGCTT TCGCTGCGCA GCTGCACGAT CTTTGTGCCC 
ACCAATGAAG CCTTCCAGCG CTACAAGAGC AAAACCGCCC ATGTGCTCTA TCACATTACC 
ACTGAGGCGT ACACCCAGAA ACGACTGCCG AATACCGTGT CATCGGACAT GGCCGGCAAT 
CCACCGCTGT ACATCACAAA GAACTCGAAT GGCGACATCT TTGTGAACAA TGCCCGGATC 
ATACCCTCGC TCAGTGTGGA GACAAACAGC GATGGCAAGC GGCAGATCAT GCACATCATC 
GACGAGGTAC TGGAGCCGCT CACCGTCAAG GCTGGCCATT CGGATACCCC CAACAATCCG 
AATGCTCTCA AGTTCCTGAA GAACGCCGAG GAGTTCAACG TGGACAACAT CGGTGTGCGC 
ACGTACCGCA GCCAGGTGAC GATGGCCAAG AAAGAGTCGG TCTATGATGC CGCCGGACAG 
CACACGTTCC TGGTTCCCGT CGATGAAGGC TTCAAGCTCT CGGCTCGCAG CAGCCTCGTG 
GACGGCAAGG TCATCGATGG CCATGTGATA CCAAACACTG TCATCTTCAC TGCCGCTGCC 
CAGCATGACG ATCCCAAGGC TTCCGCCGCT TTTGAGGACT TACTCAAGGT CACCGTCAGT 
TTCTTCAAGC AGAAGAACGG CAAAATGTAC GTCAAGTCAA ACACCATTGT GGGTGATGCC 
AAACACCGCG TGGGCGTGGT TCTGGCCGAA ATCGTGAAGG CGAACATCCC AGTGAGCAAC 
GGAGTAGTCC ATCTGATCCA CCGCCCGCTG ATGATCATCG ATACGACGGT CACCCAATTC 
CTGCAGGAGA ATGCTGAGAA CGGAGCTCTG CGCAAGTTCT ACGAAGTTAT AATGGACAAT 
GGTGGAGCAG TTCTGGACGA CATCAATAGC CTGACAGAAG TGACCATTTT GGCTCCCAGC 
AATGAGGCTT GGAACTCCTC GAACATCAAC AATGTTTTGC GAGATCGGAA TAAGATGAGG 
CAGATCCTGA ACATGCATAT CATCAAGGAC CGCTTAAATG TGGACAAGAT CAGGCAGAAA 
AATGCAAATT TGATTGCCCA GGTGCCCACT GTCAACAACA ACACTTTCCT GTACTTCAAC 
GTTCGCGGTG AGGGATCGGA TACCGTGATA ACAGTTGAGG GAGGCGGCGT GAATGCCACC 
GTTATCCAGG CTGATGTGGC CCAGACTAAT GGTTATGTTC ACATCATCGA CCATGTGCTG 
GGCGTGCCTT ACACTACAGT TCTTGGCAAA CTTGAATCCG ATCCCATGAT GAGTGACACC 
TATAAGATGG GAAAATTCTC GCACTTTAAT GACCAGCTGA ACAACACACA ACGCCGCTTC 
ACCTACTTTG TGCCCAGGGA CAAGGGCTGG CAGAAGACCG AGCTGGATTA CCCATCGGCT 
CACAAGAAGC TTTTTATGGC CGACTTTTCC TATCATTCCA AGTCCATTCT GGAGCGTCAT 
TTGGCTATTT CGGATAAGGA GTACACCATG AAGGATCTGG TTAAGTTTTC GCAAGAATCG 
GGCAGCGTAA TCCTACCCAC GTTCCGCGAC TCTTTGAGTA TCCGCGTGGA GGAGGAAGCT 
GGACGCTATG TGATCATTTG GAACTACAAG AAGATCAACG TATACCGGCC CGATGTTGAG 
TGCACCAACG GAATTATCCA CGTCATCGAC TACCCACTCC TGGAGGAAAA GGATGTGGTC 
GTGGCCGGAG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138065.1
CDS	196..2070
Translation	MLNAAALLLALLCAANAAAAADLADKLRDDSELSQFYSLLESNQIANSTLSLRSCTIFVPTNEAFQRYKSKTAHVLYHITTEAYTQKRLPNTVSSDMAGNPPLYITKNSNGDIFVNNARIIPSLSVETNSDGKRQIMHIIDEVLEPLTVKAGHSDTPNNPNALKFLKNAEEFNVDNIGVRTYRSQVTMAKKESVYDAAGQHTFLVPVDEGFKLSARSSLVDGKVIDGHVIPNTVIFTAAAQHDDPKASAAFEDLLKVTVSFFKQKNGKMYVKSNTIVGDAKHRVGVVLAEIVKANIPVSNGVVHLIHRPLMIIDTTVTQFLQENAENGALRKFYEVIMDNGGAVLDDINSLTEVTILAPSNEAWNSSNINNVLRDRNKMRQILNMHIIKDRLNVDKIRQKNANLIAQVPTVNNNTFLYFNVRGEGSDTVITVEGGGVNATVIQADVAQTNGYVHIIDHVLGVPYTTVLGKLESDPMMSDTYKMGKFSHFNDQLNNTQRRFTYFVPRDKGWQKTELDYPSAHKKLFMADFSYHSKSILERHLAISDKEYTMKDLVKFSQESGSVILPTFRDSLSIRVEEEAGRYVIIWNYKKINVYRPDVECTNGIIHVIDYPLLEEKDVVVAGG

Target ORF information:

RefSeq Version	NM_001144593.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fasciclin 1, transcript variant E (Fas1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144593.2

ORF Insert Sequence:

ATGCTGAACG CTGCAGCGCT GCTTTTGGCG CTGCTCTGCG CCGCGAACGC AGCCGCCGCC 
GCCGATTTGG CGGACAAATT GCGCGATGAT TCGGAACTCT CTCAGTTCTA CAGCCTGCTG 
GAGAGCAATC AAATTGCCAA CTCAACGCTT TCGCTGCGCA GCTGCACGAT CTTTGTGCCC 
ACCAATGAAG CCTTCCAGCG CTACAAGAGC AAAACCGCCC ATGTGCTCTA TCACATTACC 
ACTGAGGCGT ACACCCAGAA ACGACTGCCG AATACCGTGT CATCGGACAT GGCCGGCAAT 
CCACCGCTGT ACATCACAAA GAACTCGAAT GGCGACATCT TTGTGAACAA TGCCCGGATC 
ATACCCTCGC TCAGTGTGGA GACAAACAGC GATGGCAAGC GGCAGATCAT GCACATCATC 
GACGAGGTAC TGGAGCCGCT CACCGTCAAG GCTGGCCATT CGGATACCCC CAACAATCCG 
AATGCTCTCA AGTTCCTGAA GAACGCCGAG GAGTTCAACG TGGACAACAT CGGTGTGCGC 
ACGTACCGCA GCCAGGTGAC GATGGCCAAG AAAGAGTCGG TCTATGATGC CGCCGGACAG 
CACACGTTCC TGGTTCCCGT CGATGAAGGC TTCAAGCTCT CGGCTCGCAG CAGCCTCGTG 
GACGGCAAGG TCATCGATGG CCATGTGATA CCAAACACTG TCATCTTCAC TGCCGCTGCC 
CAGCATGACG ATCCCAAGGC TTCCGCCGCT TTTGAGGACT TACTCAAGGT CACCGTCAGT 
TTCTTCAAGC AGAAGAACGG CAAAATGTAC GTCAAGTCAA ACACCATTGT GGGTGATGCC 
AAACACCGCG TGGGCGTGGT TCTGGCCGAA ATCGTGAAGG CGAACATCCC AGTGAGCAAC 
GGAGTAGTCC ATCTGATCCA CCGCCCGCTG ATGATCATCG ATACGACGGT CACCCAATTC 
CTGCAGGAGA ATGCTGAGAA CGGAGCTCTG CGCAAGTTCT ACGAAGTTAT AATGGACAAT 
GGTGGAGCAG TTCTGGACGA CATCAATAGC CTGACAGAAG TGACCATTTT GGCTCCCAGC 
AATGAGGCTT GGAACTCCTC GAACATCAAC AATGTTTTGC GAGATCGGAA TAAGATGAGG 
CAGATCCTGA ACATGCATAT CATCAAGGAC CGCTTAAATG TGGACAAGAT CAGGCAGAAA 
AATGCAAATT TGATTGCCCA GGTGCCCACT GTCAACAACA ACACTTTCCT GTACTTCAAC 
GTTCGCGGTG AGGGATCGGA TACCGTGATA ACAGTTGAGG GAGGCGGCGT GAATGCCACC 
GTTATCCAGG CTGATGTGGC CCAGACTAAT GGTTATGTTC ACATCATCGA CCATGTGCTG 
GGCGTGCCTT ACACTACAGT TCTTGGCAAA CTTGAATCCG ATCCCATGAT GAGTGACACC 
TATAAGATGG GAAAATTCTC GCACTTTAAT GACCAGCTGA ACAACACACA ACGCCGCTTC 
ACCTACTTTG TGCCCAGGGA CAAGGGCTGG CAGAAGACCG AGCTGGATTA CCCATCGGCT 
CACAAGAAGC TTTTTATGGC CGACTTTTCC TATCATTCCA AGTCCATTCT GGAGCGTCAT 
TTGGCTATTT CGGATAAGGA GTACACCATG AAGGATCTGG TTAAGTTTTC GCAAGAATCG 
GGCAGCGTAA TCCTACCCAC GTTCCGCGAC TCTTTGAGTA TCCGCGTGGA GGAGGAAGCT 
GGACGCTATG TGATCATTTG GAACTACAAG AAGATCAACG TATACCGGCC CGATGTTGAG 
TGCACCAACG GAATTATCCA CGTCATCGAC TACCCACTCC TGGAGGAAAA GGATGTGGTC 
GTGGCCGGAG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Fas1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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