Pbp45 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pbp45
Entrez Gene ID	41920
Full Name	PSEA-binding protein 45kD
Synonyms	CG9597,DmPBP,DmPBP45,DmSNAP43,DmSNAPc,Dmel\CG9597,SNAP43
General protein information	Preferred Names PSEA-binding protein 45kD Names CG9597 gene product from transcript CG9597-RA CG9597-PA PSEA-binding protein Pbp45-PA drosophila melanogaster PSE-binding protein 45-kDa subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89A5-89A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_142242.3	NP_650499.1	PSEA-binding protein 45kD

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results have identified two clusters of residues in a novel DNA-binding domain required for the sequence-specific DNA-binding activity of DmSNAPc.
Title: Localization of residues in a novel DNA-binding domain of DmSNAP43 required for DmSNAPc DNA-binding activity.

Data help to establish the orientation of each SNAPc subunit on DNA and reveal cases in which different domains of the subunits SNAP43, 50, and 190 differentially contact the U1 versus U6 proximal sequence elements A.
Title: Identification of SNAPc subunit domains that interact with specific nucleotide positions in the U1 and U6 gene promoters.

These results indicate that the non-conserved C-terminal domain of DmSNAP43 plays a critical role in DmSNAPc binding to the PSEA, even though this region is not required for DmSNAP43 to assemble into a complex with both DmSNAP190 and DmSNAP50.
Title: A map of Drosophila melanogaster small nuclear RNA-activating protein complex (DmSNAPc) domains involved in subunit assembly and DNA binding.

Results support a model in which conformational differences in proximal sequence element binding protein (DmPBP)-DNA complexes lead to selective RNA polymerase recruitment to U1 and U6 promoters.
Title: Architectural arrangement of cloned proximal sequence element-binding protein subunits on Drosophila U1 and U6 snRNA gene promoters.

The following Pbp45 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pbp45 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05044	NM_142242.3 Latest version!	Drosophila melanogaster PSEA-binding protein 45kD (Pbp45), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pbp45 ( NM_142242.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650499.1 Drosophila melanogaster PSEA-binding protein 45kD (Pbp45), mRNA.

Price & Availability↑

CloneID	OFa05044
Clone ID Related Accession (Same CDS sequence)	NM_142242.3
Accession Version	NM_142242.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PSEA-binding protein 45kD
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142242.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCTGA ATATCTTTGA CGACTGCTGG GAGCTGGTGC AACGATTTCA GCGATTGGTT 
AATGATGGCG AAAACTGCGA GTTCGAGGTG TTCTGCCGGT GCTGGCGAGA ACTGCAGCTG 
CAGCACCTCT TCACTGCCCA GACGAACCAC ACAGAGGTGA TAGCCACCAC ACTGGCGGCC 
CTGCATGTGG CCAAGCGACT GTCGTGCTCC CGACGCACCA CCGGGGACGT TTTCCCGGCA 
TCTCGCGCTC AAAGGATCGG AGGTTTCTTT CTGCTCTACG TAATCTACTA CAAGCAGCCC 
ACGCACAACT TTATTAAGAT CGAGGTCTCA CCGCGCACTT GGCAAGAACT AACAGACTAC 
GCTCTAGATC TGCGCAAGGA TAGTCCGGAG CGGAAGGACA CTCATCAGAT CGCCTACATG 
CTGTGGCGCC TGACCCAGGA GCAGGCCTTC CGCTTCACCG CGCTCGACTA TTGCCAGGGG 
TTGGACAATC TGGTGGACTA CGACCGTGTG GAGACCGTAG CGGGTGCCAA GGAACAGAGG 
CAGAGTGCCT TGATGCAGAA GCAACAGCGT GCGAACGGCG TCAGTCTCAC ATACGAACTG 
GAGGGTCTGC GAGCACTGGA CCAGGCAAGC CAGCCATTGT GTGAACTGGA AGCGGCATAC 
AATGCCCAAA AGAAGCAATT GGCGGCTGGT CATGAGCACG CTTTGCCGCC CTCTCAAATA 
TTCGGCCATT TGCGAGAAGT CTTTGCCGAT ATCCAAAGTG TTCTAGGAGC TAGAAAGAGT 
ACTCCAGATG AGAATTGTAC CACAACATCT ACAGGCAACC AGTTGGAAGT GCGCCAGAGG 
GTGCGGAACA AGGCCATGTA CGGCGTCGAG GAACGGGAGC CGCAAAACCA GGCGGATGAA 
CTAGAAGTGC AGCTGGAGGT CAACGAGACT TATCAACGCC GCATGTCCTC GGCCACCGTT 
TTTCAGAGGG AACTTCCAGC AGACGTGCAG CAAGAGTATG AGATGATTGA GTTTAGTGAC 
GACGAGGAAA TGGAAGTGGG TGAAAGCGAG GAGGTCACGG AAGAAGAACT CAAAGCTATT 
TTGGATACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650499.1
CDS	104..1195
Translation	MELNIFDDCWELVQRFQRLVNDGENCEFEVFCRCWRELQLQHLFTAQTNHTEVIATTLAALHVAKRLSCSRRTTGDVFPASRAQRIGGFFLLYVIYYKQPTHNFIKIEVSPRTWQELTDYALDLRKDSPERKDTHQIAYMLWRLTQEQAFRFTALDYCQGLDNLVDYDRVETVAGAKEQRQSALMQKQQRANGVSLTYELEGLRALDQASQPLCELEAAYNAQKKQLAAGHEHALPPSQIFGHLREVFADIQSVLGARKSTPDENCTTTSTGNQLEVRQRVRNKAMYGVEEREPQNQADELEVQLEVNETYQRRMSSATVFQRELPADVQQEYEMIEFSDDEEMEVGESEEVTEEELKAILDT

Target ORF information:

RefSeq Version	NM_142242.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PSEA-binding protein 45kD (Pbp45), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142242.3

ORF Insert Sequence:

ATGGAGCTGA ATATCTTTGA CGACTGCTGG GAGCTGGTGC AACGATTTCA GCGATTGGTT 
AATGATGGCG AAAACTGCGA GTTCGAGGTG TTCTGCCGGT GCTGGCGAGA ACTGCAGCTG 
CAGCACCTCT TCACTGCCCA GACGAACCAC ACAGAGGTGA TAGCCACCAC ACTGGCGGCC 
CTGCATGTGG CCAAGCGACT GTCGTGCTCC CGACGCACCA CCGGGGACGT TTTCCCGGCA 
TCTCGCGCTC AAAGGATCGG AGGTTTCTTT CTGCTCTACG TAATCTACTA CAAGCAGCCC 
ACGCACAACT TTATTAAGAT CGAGGTCTCA CCGCGCACTT GGCAAGAACT AACAGACTAC 
GCTCTAGATC TGCGCAAGGA TAGTCCGGAG CGGAAGGACA CTCATCAGAT CGCCTACATG 
CTGTGGCGCC TGACCCAGGA GCAGGCCTTC CGCTTCACCG CGCTCGACTA TTGCCAGGGG 
TTGGACAATC TGGTGGACTA CGACCGTGTG GAGACCGTAG CGGGTGCCAA GGAACAGAGG 
CAGAGTGCCT TGATGCAGAA GCAACAGCGT GCGAACGGCG TCAGTCTCAC ATACGAACTG 
GAGGGTCTGC GAGCACTGGA CCAGGCAAGC CAGCCATTGT GTGAACTGGA AGCGGCATAC 
AATGCCCAAA AGAAGCAATT GGCGGCTGGT CATGAGCACG CTTTGCCGCC CTCTCAAATA 
TTCGGCCATT TGCGAGAAGT CTTTGCCGAT ATCCAAAGTG TTCTAGGAGC TAGAAAGAGT 
ACTCCAGATG AGAATTGTAC CACAACATCT ACAGGCAACC AGTTGGAAGT GCGCCAGAGG 
GTGCGGAACA AGGCCATGTA CGGCGTCGAG GAACGGGAGC CGCAAAACCA GGCGGATGAA 
CTAGAAGTGC AGCTGGAGGT CAACGAGACT TATCAACGCC GCATGTCCTC GGCCACCGTT 
TTTCAGAGGG AACTTCCAGC AGACGTGCAG CAAGAGTATG AGATGATTGA GTTTAGTGAC 
GACGAGGAAA TGGAAGTGGG TGAAAGCGAG GAGGTCACGG AAGAAGAACT CAAAGCTATT 
TTGGATACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Pbp45 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報