nsl1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following nsl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nsl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa08408 NM_001170153.3
Latest version!
Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa08409 NM_001260195.2
Latest version!
Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa08410 NM_001260194.2
Latest version!
Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa05036 NM_142235.4
Latest version!
Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa05037 NM_001170152.2
Latest version!
Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa05037 NM_001170150.3
Latest version!
Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa05037 NM_169673.3
Latest version!
Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa05038 NM_169672.4
Latest version!
Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant M, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa08406 NM_001275684.1
Latest version!
Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant P, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa08406 NM_001170151.3
Latest version!
Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa08407 NM_001260197.2
Latest version!
Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant O, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa08411 NM_001260196.2
Latest version!
Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant N, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa08408
    Clone ID Related Accession (Same CDS sequence) NM_001170153.3
    Accession Version NM_001170153.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2811bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product non-specific lethal 1, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170153.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG GTACTGCTAC TACTTCTTCT
    TCTACTACGC CTACTACAAC GCCACTTGTG GCCAACAGTA AATTATTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163624.1
    CDS251..3061
    Translation

    Target ORF information:

    RefSeq Version NM_001170153.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170153.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG GTACTGCTAC TACTTCTTCT
    TCTACTACGC CTACTACAAC GCCACTTGTG GCCAACAGTA AATTATTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08409
    Clone ID Related Accession (Same CDS sequence) NM_001260195.2
    Accession Version NM_001260195.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product non-specific lethal 1, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260195.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG GTACTGCTAC TACTTCTTCT
    TCTACTACGC CTACTACAAC GCCACTTGTG GCCAACAGTA AATTATTCGG CCAGGGGCAA
    GCGGCAACCA CAACACCCAA CTGGAGCGAA CAGTTCCAGC TCGCTGTGGC CAGACTCTCG
    CCAACGCCAA CGACAACGCC ACCACAGTCC CTCCTCAACC TCGATCTCGG CACTGAGCGG
    CGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247124.1
    CDS251..3196
    Translation

    Target ORF information:

    RefSeq Version NM_001260195.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001260195.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG GTACTGCTAC TACTTCTTCT
    TCTACTACGC CTACTACAAC GCCACTTGTG GCCAACAGTA AATTATTCGG CCAGGGGCAA
    GCGGCAACCA CAACACCCAA CTGGAGCGAA CAGTTCCAGC TCGCTGTGGC CAGACTCTCG
    CCAACGCCAA CGACAACGCC ACCACAGTCC CTCCTCAACC TCGATCTCGG CACTGAGCGG
    CGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08410
    Clone ID Related Accession (Same CDS sequence) NM_001260194.2
    Accession Version NM_001260194.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product non-specific lethal 1, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260194.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG GTACTGCTAC TACTTCTTCT
    TCTACTACGC CTACTACAAC GCCACTTGTG GCCAACAGTA AATTATTCAG CGGCCAGGGG
    CAAGCGGCAA CCACAACACC CAACTGGAGC GAACAGTTCC AGCTCGCTGT GGCCAGACTC
    TCGCCAACGC CAACGACAAC GCCACCACAG TCCCTCCTCA ACCTCGATCT CGGCACTGAG
    CGGCGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247123.1
    CDS251..3199
    Translation

    Target ORF information:

    RefSeq Version NM_001260194.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001260194.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG GTACTGCTAC TACTTCTTCT
    TCTACTACGC CTACTACAAC GCCACTTGTG GCCAACAGTA AATTATTCAG CGGCCAGGGG
    CAAGCGGCAA CCACAACACC CAACTGGAGC GAACAGTTCC AGCTCGCTGT GGCCAGACTC
    TCGCCAACGC CAACGACAAC GCCACCACAG TCCCTCCTCA ACCTCGATCT CGGCACTGAG
    CGGCGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05036
    Clone ID Related Accession (Same CDS sequence) NM_142235.4
    Accession Version NM_142235.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4647bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product non-specific lethal 1, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142235.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGCGGCCA GGGGCAAGCG GCAACCACAA
    CACCCAACTG GAGCGAACAG TTCCAGCTCG CTGTGGCCAG ACTCTCGCCA ACGCCAACGA
    CAACGCCACC ACAGTCCCTC CTCAACCTCG ATCTCGGCAC TGAGCGGCGC TAAGAAGGCA
    CGCAAATCCG CAACCAACAG CAGCAGCAGC AATTCCACAC AGCAGCAGCA GAATCACCAC
    GGCACCAAAA TCAACAATCA TCATCTCAAT GGCTACGGCG ATCAGTGGGG GGAGCAAAGC
    AGGAGTCGTC GCAACTCTTC GCCCTCCAAC AGTCATAAAA ATGAGAGAAC CTCTGAGCGT
    CGCGTTCGTC CCATCTACGA CATCAATAAC ATCGTTATAC CCTACAGTAT GCTAGCCCAG
    TCGAAAATGG AGATAATTCC CTACAAGGAA ATACCCATAC CCAAGTGGCG CATTGTAGAC
    AGTGATAACG ATAAGGGAAA ACATTCGTCA GATGAGTCAA TGGAGCGCAA ACTGAGCAAT
    GGCTGTGTCG CATCAAAGTC AGAGGAGCTA GCCAAACAAC AGCTACCACC ACCACCAGAT
    CAAGATCAAG AGCAGCCGGC CCTGCAGCCT CCGAAGGTTA ATGGTTTAAA GGAAAATCAA
    GCAGTCAAGC ACAATAACAA TAATAATACA AACAATAATA ATAATAAAAA TGGACTGGTA
    AATGGTAACG CCAAAAAGGA TGAACTTAAG GCTGCAGAGG ATCACGATAC GCAATCAAAA
    GAAGACGACG ATCCAAAGGA AAAGGTGGTC AAACCCAAGC TCAACGGAAA CTTGATAAAG
    AATCCGATTG ACAAAACTGC CGAAACGCAA GACAAAATTG CTCAGCCAGC ACCAGAACCA
    CCACTGCCAA AGCGACCCAA GCTGGAAACT CCAACCTCCA ACGAGGTCAC CAAGGCTAAT
    GGGCAGTTGG TGCAGCAACA GCTGGAATCC CGTGAGAGCG AGGAGGAGCC ACAGCATGTC
    GACGAAGATC TTTCGGATGA AGCGTTTATT ATGCGACATC AGCGAGCTCT TCTCGAGGAG
    CGACGCCGCT TCGAGACTTT CCTTAAGTTC CCCTGGAGCA CCCGATCGCG TGCAAATAGG
    CGCGTCGATA GTCGTGCCGA ATCTAGTGGC GCCAACACAC CGGATCCCGC CTCACCAGCT
    CCTCATCTAG GCGGCCCTGG TCACGACAAC GAAAGCATTC CCTCGCCGCT GGCGTACCCA
    CTGGACGCTT TTAACGAAAG CGGTGAGCTG CTGACTGGCG GACAGGCACG CCAAGCTCGT
    CGTCGGACTA CGTCTAGCAA GCTTAAGGAT CAGCTGGAAC GGCGCAGCAC CACGCCCGAT
    CTGCGGGAGT CTTACACTGT GATGCCATCT CCCTTTGAGC CCTTGAACTT TCCGCTCTCC
    GACGAGGTCT ACCAGCGATT GCTAGCCGAG ACATATGCGC CGCCCAGGTC AATGACCGTC
    TCAAAGCGGG CCAAAAGCAA GTCGATATCC TCGAACTGTG ATGGTGGGAA CACCTTCACA
    GGAGGCAGCG GCAGTCGGCG CAGCAGCAAG TGCAAGATTA AGCCAAATGG TCAGCTAAAT
    GGCCAGCTCA ATGGCCACCC TGCGGCGGCG AAGTTGAACG GAATAGATGC GGCAAATGAA
    GGTGGAATTT CTGAGCCGGA AGAGGAAGAG ATGCTGCTAG AAGACGAGGA TGACGACTAT
    CCCAAGCATC ATCTGGCGCC GCTGGATGAC GAGGAGCCGT CGACACCAGA TGTCGAGCAT
    GAGCTTTACG ACTCGGCGAT CGATGCCTAT CTCGCTGATC CGGAGGCGCT AGAGGAGGAT
    ATGGGCGAGG ATCCCTTCGA GGACGACGAT CCCAACGATC CGGAGTGGAA GACACGGACC
    GAGGGTGTTC GCAGCAGACG CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_650492.2
    CDS327..4973
    Translation

    Target ORF information:

    RefSeq Version NM_142235.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_142235.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGCGGCCA GGGGCAAGCG GCAACCACAA
    CACCCAACTG GAGCGAACAG TTCCAGCTCG CTGTGGCCAG ACTCTCGCCA ACGCCAACGA
    CAACGCCACC ACAGTCCCTC CTCAACCTCG ATCTCGGCAC TGAGCGGCGC TAAGAAGGCA
    CGCAAATCCG CAACCAACAG CAGCAGCAGC AATTCCACAC AGCAGCAGCA GAATCACCAC
    GGCACCAAAA TCAACAATCA TCATCTCAAT GGCTACGGCG ATCAGTGGGG GGAGCAAAGC
    AGGAGTCGTC GCAACTCTTC GCCCTCCAAC AGTCATAAAA ATGAGAGAAC CTCTGAGCGT
    CGCGTTCGTC CCATCTACGA CATCAATAAC ATCGTTATAC CCTACAGTAT GCTAGCCCAG
    TCGAAAATGG AGATAATTCC CTACAAGGAA ATACCCATAC CCAAGTGGCG CATTGTAGAC
    AGTGATAACG ATAAGGGAAA ACATTCGTCA GATGAGTCAA TGGAGCGCAA ACTGAGCAAT
    GGCTGTGTCG CATCAAAGTC AGAGGAGCTA GCCAAACAAC AGCTACCACC ACCACCAGAT
    CAAGATCAAG AGCAGCCGGC CCTGCAGCCT CCGAAGGTTA ATGGTTTAAA GGAAAATCAA
    GCAGTCAAGC ACAATAACAA TAATAATACA AACAATAATA ATAATAAAAA TGGACTGGTA
    AATGGTAACG CCAAAAAGGA TGAACTTAAG GCTGCAGAGG ATCACGATAC GCAATCAAAA
    GAAGACGACG ATCCAAAGGA AAAGGTGGTC AAACCCAAGC TCAACGGAAA CTTGATAAAG
    AATCCGATTG ACAAAACTGC CGAAACGCAA GACAAAATTG CTCAGCCAGC ACCAGAACCA
    CCACTGCCAA AGCGACCCAA GCTGGAAACT CCAACCTCCA ACGAGGTCAC CAAGGCTAAT
    GGGCAGTTGG TGCAGCAACA GCTGGAATCC CGTGAGAGCG AGGAGGAGCC ACAGCATGTC
    GACGAAGATC TTTCGGATGA AGCGTTTATT ATGCGACATC AGCGAGCTCT TCTCGAGGAG
    CGACGCCGCT TCGAGACTTT CCTTAAGTTC CCCTGGAGCA CCCGATCGCG TGCAAATAGG
    CGCGTCGATA GTCGTGCCGA ATCTAGTGGC GCCAACACAC CGGATCCCGC CTCACCAGCT
    CCTCATCTAG GCGGCCCTGG TCACGACAAC GAAAGCATTC CCTCGCCGCT GGCGTACCCA
    CTGGACGCTT TTAACGAAAG CGGTGAGCTG CTGACTGGCG GACAGGCACG CCAAGCTCGT
    CGTCGGACTA CGTCTAGCAA GCTTAAGGAT CAGCTGGAAC GGCGCAGCAC CACGCCCGAT
    CTGCGGGAGT CTTACACTGT GATGCCATCT CCCTTTGAGC CCTTGAACTT TCCGCTCTCC
    GACGAGGTCT ACCAGCGATT GCTAGCCGAG ACATATGCGC CGCCCAGGTC AATGACCGTC
    TCAAAGCGGG CCAAAAGCAA GTCGATATCC TCGAACTGTG ATGGTGGGAA CACCTTCACA
    GGAGGCAGCG GCAGTCGGCG CAGCAGCAAG TGCAAGATTA AGCCAAATGG TCAGCTAAAT
    GGCCAGCTCA ATGGCCACCC TGCGGCGGCG AAGTTGAACG GAATAGATGC GGCAAATGAA
    GGTGGAATTT CTGAGCCGGA AGAGGAAGAG ATGCTGCTAG AAGACGAGGA TGACGACTAT
    CCCAAGCATC ATCTGGCGCC GCTGGATGAC GAGGAGCCGT CGACACCAGA TGTCGAGCAT
    GAGCTTTACG ACTCGGCGAT CGATGCCTAT CTCGCTGATC CGGAGGCGCT AGAGGAGGAT
    ATGGGCGAGG ATCCCTTCGA GGACGACGAT CCCAACGATC CGGAGTGGAA GACACGGACC
    GAGGGTGTTC GCAGCAGACG CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05037
    Clone ID Related Accession (Same CDS sequence) NM_001170152.2 , NM_001170150.3 , NM_169673.3
    Accession Version NM_001170152.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4653bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product non-specific lethal 1, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170152.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG CGGCCAGGGG CAAGCGGCAA
    CCACAACACC CAACTGGAGC GAACAGTTCC AGCTCGCTGT GGCCAGACTC TCGCCAACGC
    CAACGACAAC GCCACCACAG TCCCTCCTCA ACCTCGATCT CGGCACTGAG CGGCGCTAAG
    AAGGCACGCA AATCCGCAAC CAACAGCAGC AGCAGCAATT CCACACAGCA GCAGCAGAAT
    CACCACGGCA CCAAAATCAA CAATCATCAT CTCAATGGCT ACGGCGATCA GTGGGGGGAG
    CAAAGCAGGA GTCGTCGCAA CTCTTCGCCC TCCAACAGTC ATAAAAATGA GAGAACCTCT
    GAGCGTCGCG TTCGTCCCAT CTACGACATC AATAACATCG TTATACCCTA CAGTATGCTA
    GCCCAGTCGA AAATGGAGAT AATTCCCTAC AAGGAAATAC CCATACCCAA GTGGCGCATT
    GTAGACAGTG ATAACGATAA GGGAAAACAT TCGTCAGATG AGTCAATGGA GCGCAAACTG
    AGCAATGGCT GTGTCGCATC AAAGTCAGAG GAGCTAGCCA AACAACAGCT ACCACCACCA
    CCAGATCAAG ATCAAGAGCA GCCGGCCCTG CAGCCTCCGA AGGTTAATGG TTTAAAGGAA
    AATCAAGCAG TCAAGCACAA TAACAATAAT AATACAAACA ATAATAATAA TAAAAATGGA
    CTGGTAAATG GTAACGCCAA AAAGGATGAA CTTAAGGCTG CAGAGGATCA CGATACGCAA
    TCAAAAGAAG ACGACGATCC AAAGGAAAAG GTGGTCAAAC CCAAGCTCAA CGGAAACTTG
    ATAAAGAATC CGATTGACAA AACTGCCGAA ACGCAAGACA AAATTGCTCA GCCAGCACCA
    GAACCACCAC TGCCAAAGCG ACCCAAGCTG GAAACTCCAA CCTCCAACGA GGTCACCAAG
    GCTAATGGGC AGTTGGTGCA GCAACAGCTG GAATCCCGTG AGAGCGAGGA GGAGCCACAG
    CATGTCGACG AAGATCTTTC GGATGAAGCG TTTATTATGC GACATCAGCG AGCTCTTCTC
    GAGGAGCGAC GCCGCTTCGA GACTTTCCTT AAGTTCCCCT GGAGCACCCG ATCGCGTGCA
    AATAGGCGCG TCGATAGTCG TGCCGAATCT AGTGGCGCCA ACACACCGGA TCCCGCCTCA
    CCAGCTCCTC ATCTAGGCGG CCCTGGTCAC GACAACGAAA GCATTCCCTC GCCGCTGGCG
    TACCCACTGG ACGCTTTTAA CGAAAGCGGT GAGCTGCTGA CTGGCGGACA GGCACGCCAA
    GCTCGTCGTC GGACTACGTC TAGCAAGCTT AAGGATCAGC TGGAACGGCG CAGCACCACG
    CCCGATCTGC GGGAGTCTTA CACTGTGATG CCATCTCCCT TTGAGCCCTT GAACTTTCCG
    CTCTCCGACG AGGTCTACCA GCGATTGCTA GCCGAGACAT ATGCGCCGCC CAGGTCAATG
    ACCGTCTCAA AGCGGGCCAA AAGCAAGTCG ATATCCTCGA ACTGTGATGG TGGGAACACC
    TTCACAGGAG GCAGCGGCAG TCGGCGCAGC AGCAAGTGCA AGATTAAGCC AAATGGTCAG
    CTAAATGGCC AGCTCAATGG CCACCCTGCG GCGGCGAAGT TGAACGGAAT AGATGCGGCA
    AATGAAGGTG GAATTTCTGA GCCGGAAGAG GAAGAGATGC TGCTAGAAGA CGAGGATGAC
    GACTATCCCA AGCATCATCT GGCGCCGCTG GATGACGAGG AGCCGTCGAC ACCAGATGTC
    GAGCATGAGC TTTACGACTC GGCGATCGAT GCCTATCTCG CTGATCCGGA GGCGCTAGAG
    GAGGATATGG GCGAGGATCC CTTCGAGGAC GACGATCCCA ACGATCCGGA GTGGAAGACA
    CGGACCGAGG GTGTTCGCAG CAGACGCATC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163623.1
    CDS351..5003
    Translation

    Target ORF information:

    RefSeq Version NM_001170152.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170152.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG CGGCCAGGGG CAAGCGGCAA
    CCACAACACC CAACTGGAGC GAACAGTTCC AGCTCGCTGT GGCCAGACTC TCGCCAACGC
    CAACGACAAC GCCACCACAG TCCCTCCTCA ACCTCGATCT CGGCACTGAG CGGCGCTAAG
    AAGGCACGCA AATCCGCAAC CAACAGCAGC AGCAGCAATT CCACACAGCA GCAGCAGAAT
    CACCACGGCA CCAAAATCAA CAATCATCAT CTCAATGGCT ACGGCGATCA GTGGGGGGAG
    CAAAGCAGGA GTCGTCGCAA CTCTTCGCCC TCCAACAGTC ATAAAAATGA GAGAACCTCT
    GAGCGTCGCG TTCGTCCCAT CTACGACATC AATAACATCG TTATACCCTA CAGTATGCTA
    GCCCAGTCGA AAATGGAGAT AATTCCCTAC AAGGAAATAC CCATACCCAA GTGGCGCATT
    GTAGACAGTG ATAACGATAA GGGAAAACAT TCGTCAGATG AGTCAATGGA GCGCAAACTG
    AGCAATGGCT GTGTCGCATC AAAGTCAGAG GAGCTAGCCA AACAACAGCT ACCACCACCA
    CCAGATCAAG ATCAAGAGCA GCCGGCCCTG CAGCCTCCGA AGGTTAATGG TTTAAAGGAA
    AATCAAGCAG TCAAGCACAA TAACAATAAT AATACAAACA ATAATAATAA TAAAAATGGA
    CTGGTAAATG GTAACGCCAA AAAGGATGAA CTTAAGGCTG CAGAGGATCA CGATACGCAA
    TCAAAAGAAG ACGACGATCC AAAGGAAAAG GTGGTCAAAC CCAAGCTCAA CGGAAACTTG
    ATAAAGAATC CGATTGACAA AACTGCCGAA ACGCAAGACA AAATTGCTCA GCCAGCACCA
    GAACCACCAC TGCCAAAGCG ACCCAAGCTG GAAACTCCAA CCTCCAACGA GGTCACCAAG
    GCTAATGGGC AGTTGGTGCA GCAACAGCTG GAATCCCGTG AGAGCGAGGA GGAGCCACAG
    CATGTCGACG AAGATCTTTC GGATGAAGCG TTTATTATGC GACATCAGCG AGCTCTTCTC
    GAGGAGCGAC GCCGCTTCGA GACTTTCCTT AAGTTCCCCT GGAGCACCCG ATCGCGTGCA
    AATAGGCGCG TCGATAGTCG TGCCGAATCT AGTGGCGCCA ACACACCGGA TCCCGCCTCA
    CCAGCTCCTC ATCTAGGCGG CCCTGGTCAC GACAACGAAA GCATTCCCTC GCCGCTGGCG
    TACCCACTGG ACGCTTTTAA CGAAAGCGGT GAGCTGCTGA CTGGCGGACA GGCACGCCAA
    GCTCGTCGTC GGACTACGTC TAGCAAGCTT AAGGATCAGC TGGAACGGCG CAGCACCACG
    CCCGATCTGC GGGAGTCTTA CACTGTGATG CCATCTCCCT TTGAGCCCTT GAACTTTCCG
    CTCTCCGACG AGGTCTACCA GCGATTGCTA GCCGAGACAT ATGCGCCGCC CAGGTCAATG
    ACCGTCTCAA AGCGGGCCAA AAGCAAGTCG ATATCCTCGA ACTGTGATGG TGGGAACACC
    TTCACAGGAG GCAGCGGCAG TCGGCGCAGC AGCAAGTGCA AGATTAAGCC AAATGGTCAG
    CTAAATGGCC AGCTCAATGG CCACCCTGCG GCGGCGAAGT TGAACGGAAT AGATGCGGCA
    AATGAAGGTG GAATTTCTGA GCCGGAAGAG GAAGAGATGC TGCTAGAAGA CGAGGATGAC
    GACTATCCCA AGCATCATCT GGCGCCGCTG GATGACGAGG AGCCGTCGAC ACCAGATGTC
    GAGCATGAGC TTTACGACTC GGCGATCGAT GCCTATCTCG CTGATCCGGA GGCGCTAGAG
    GAGGATATGG GCGAGGATCC CTTCGAGGAC GACGATCCCA ACGATCCGGA GTGGAAGACA
    CGGACCGAGG GTGTTCGCAG CAGACGCATC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05037
    Clone ID Related Accession (Same CDS sequence) NM_001170152.2 , NM_001170150.3 , NM_169673.3
    Accession Version NM_001170150.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4653bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product non-specific lethal 1, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170150.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG CGGCCAGGGG CAAGCGGCAA
    CCACAACACC CAACTGGAGC GAACAGTTCC AGCTCGCTGT GGCCAGACTC TCGCCAACGC
    CAACGACAAC GCCACCACAG TCCCTCCTCA ACCTCGATCT CGGCACTGAG CGGCGCTAAG
    AAGGCACGCA AATCCGCAAC CAACAGCAGC AGCAGCAATT CCACACAGCA GCAGCAGAAT
    CACCACGGCA CCAAAATCAA CAATCATCAT CTCAATGGCT ACGGCGATCA GTGGGGGGAG
    CAAAGCAGGA GTCGTCGCAA CTCTTCGCCC TCCAACAGTC ATAAAAATGA GAGAACCTCT
    GAGCGTCGCG TTCGTCCCAT CTACGACATC AATAACATCG TTATACCCTA CAGTATGCTA
    GCCCAGTCGA AAATGGAGAT AATTCCCTAC AAGGAAATAC CCATACCCAA GTGGCGCATT
    GTAGACAGTG ATAACGATAA GGGAAAACAT TCGTCAGATG AGTCAATGGA GCGCAAACTG
    AGCAATGGCT GTGTCGCATC AAAGTCAGAG GAGCTAGCCA AACAACAGCT ACCACCACCA
    CCAGATCAAG ATCAAGAGCA GCCGGCCCTG CAGCCTCCGA AGGTTAATGG TTTAAAGGAA
    AATCAAGCAG TCAAGCACAA TAACAATAAT AATACAAACA ATAATAATAA TAAAAATGGA
    CTGGTAAATG GTAACGCCAA AAAGGATGAA CTTAAGGCTG CAGAGGATCA CGATACGCAA
    TCAAAAGAAG ACGACGATCC AAAGGAAAAG GTGGTCAAAC CCAAGCTCAA CGGAAACTTG
    ATAAAGAATC CGATTGACAA AACTGCCGAA ACGCAAGACA AAATTGCTCA GCCAGCACCA
    GAACCACCAC TGCCAAAGCG ACCCAAGCTG GAAACTCCAA CCTCCAACGA GGTCACCAAG
    GCTAATGGGC AGTTGGTGCA GCAACAGCTG GAATCCCGTG AGAGCGAGGA GGAGCCACAG
    CATGTCGACG AAGATCTTTC GGATGAAGCG TTTATTATGC GACATCAGCG AGCTCTTCTC
    GAGGAGCGAC GCCGCTTCGA GACTTTCCTT AAGTTCCCCT GGAGCACCCG ATCGCGTGCA
    AATAGGCGCG TCGATAGTCG TGCCGAATCT AGTGGCGCCA ACACACCGGA TCCCGCCTCA
    CCAGCTCCTC ATCTAGGCGG CCCTGGTCAC GACAACGAAA GCATTCCCTC GCCGCTGGCG
    TACCCACTGG ACGCTTTTAA CGAAAGCGGT GAGCTGCTGA CTGGCGGACA GGCACGCCAA
    GCTCGTCGTC GGACTACGTC TAGCAAGCTT AAGGATCAGC TGGAACGGCG CAGCACCACG
    CCCGATCTGC GGGAGTCTTA CACTGTGATG CCATCTCCCT TTGAGCCCTT GAACTTTCCG
    CTCTCCGACG AGGTCTACCA GCGATTGCTA GCCGAGACAT ATGCGCCGCC CAGGTCAATG
    ACCGTCTCAA AGCGGGCCAA AAGCAAGTCG ATATCCTCGA ACTGTGATGG TGGGAACACC
    TTCACAGGAG GCAGCGGCAG TCGGCGCAGC AGCAAGTGCA AGATTAAGCC AAATGGTCAG
    CTAAATGGCC AGCTCAATGG CCACCCTGCG GCGGCGAAGT TGAACGGAAT AGATGCGGCA
    AATGAAGGTG GAATTTCTGA GCCGGAAGAG GAAGAGATGC TGCTAGAAGA CGAGGATGAC
    GACTATCCCA AGCATCATCT GGCGCCGCTG GATGACGAGG AGCCGTCGAC ACCAGATGTC
    GAGCATGAGC TTTACGACTC GGCGATCGAT GCCTATCTCG CTGATCCGGA GGCGCTAGAG
    GAGGATATGG GCGAGGATCC CTTCGAGGAC GACGATCCCA ACGATCCGGA GTGGAAGACA
    CGGACCGAGG GTGTTCGCAG CAGACGCATC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163621.1
    CDS251..4903
    Translation

    Target ORF information:

    RefSeq Version NM_001170150.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170150.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG CGGCCAGGGG CAAGCGGCAA
    CCACAACACC CAACTGGAGC GAACAGTTCC AGCTCGCTGT GGCCAGACTC TCGCCAACGC
    CAACGACAAC GCCACCACAG TCCCTCCTCA ACCTCGATCT CGGCACTGAG CGGCGCTAAG
    AAGGCACGCA AATCCGCAAC CAACAGCAGC AGCAGCAATT CCACACAGCA GCAGCAGAAT
    CACCACGGCA CCAAAATCAA CAATCATCAT CTCAATGGCT ACGGCGATCA GTGGGGGGAG
    CAAAGCAGGA GTCGTCGCAA CTCTTCGCCC TCCAACAGTC ATAAAAATGA GAGAACCTCT
    GAGCGTCGCG TTCGTCCCAT CTACGACATC AATAACATCG TTATACCCTA CAGTATGCTA
    GCCCAGTCGA AAATGGAGAT AATTCCCTAC AAGGAAATAC CCATACCCAA GTGGCGCATT
    GTAGACAGTG ATAACGATAA GGGAAAACAT TCGTCAGATG AGTCAATGGA GCGCAAACTG
    AGCAATGGCT GTGTCGCATC AAAGTCAGAG GAGCTAGCCA AACAACAGCT ACCACCACCA
    CCAGATCAAG ATCAAGAGCA GCCGGCCCTG CAGCCTCCGA AGGTTAATGG TTTAAAGGAA
    AATCAAGCAG TCAAGCACAA TAACAATAAT AATACAAACA ATAATAATAA TAAAAATGGA
    CTGGTAAATG GTAACGCCAA AAAGGATGAA CTTAAGGCTG CAGAGGATCA CGATACGCAA
    TCAAAAGAAG ACGACGATCC AAAGGAAAAG GTGGTCAAAC CCAAGCTCAA CGGAAACTTG
    ATAAAGAATC CGATTGACAA AACTGCCGAA ACGCAAGACA AAATTGCTCA GCCAGCACCA
    GAACCACCAC TGCCAAAGCG ACCCAAGCTG GAAACTCCAA CCTCCAACGA GGTCACCAAG
    GCTAATGGGC AGTTGGTGCA GCAACAGCTG GAATCCCGTG AGAGCGAGGA GGAGCCACAG
    CATGTCGACG AAGATCTTTC GGATGAAGCG TTTATTATGC GACATCAGCG AGCTCTTCTC
    GAGGAGCGAC GCCGCTTCGA GACTTTCCTT AAGTTCCCCT GGAGCACCCG ATCGCGTGCA
    AATAGGCGCG TCGATAGTCG TGCCGAATCT AGTGGCGCCA ACACACCGGA TCCCGCCTCA
    CCAGCTCCTC ATCTAGGCGG CCCTGGTCAC GACAACGAAA GCATTCCCTC GCCGCTGGCG
    TACCCACTGG ACGCTTTTAA CGAAAGCGGT GAGCTGCTGA CTGGCGGACA GGCACGCCAA
    GCTCGTCGTC GGACTACGTC TAGCAAGCTT AAGGATCAGC TGGAACGGCG CAGCACCACG
    CCCGATCTGC GGGAGTCTTA CACTGTGATG CCATCTCCCT TTGAGCCCTT GAACTTTCCG
    CTCTCCGACG AGGTCTACCA GCGATTGCTA GCCGAGACAT ATGCGCCGCC CAGGTCAATG
    ACCGTCTCAA AGCGGGCCAA AAGCAAGTCG ATATCCTCGA ACTGTGATGG TGGGAACACC
    TTCACAGGAG GCAGCGGCAG TCGGCGCAGC AGCAAGTGCA AGATTAAGCC AAATGGTCAG
    CTAAATGGCC AGCTCAATGG CCACCCTGCG GCGGCGAAGT TGAACGGAAT AGATGCGGCA
    AATGAAGGTG GAATTTCTGA GCCGGAAGAG GAAGAGATGC TGCTAGAAGA CGAGGATGAC
    GACTATCCCA AGCATCATCT GGCGCCGCTG GATGACGAGG AGCCGTCGAC ACCAGATGTC
    GAGCATGAGC TTTACGACTC GGCGATCGAT GCCTATCTCG CTGATCCGGA GGCGCTAGAG
    GAGGATATGG GCGAGGATCC CTTCGAGGAC GACGATCCCA ACGATCCGGA GTGGAAGACA
    CGGACCGAGG GTGTTCGCAG CAGACGCATC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05037
    Clone ID Related Accession (Same CDS sequence) NM_001170152.2 , NM_001170150.3 , NM_169673.3
    Accession Version NM_169673.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4653bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product non-specific lethal 1, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169673.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG CGGCCAGGGG CAAGCGGCAA
    CCACAACACC CAACTGGAGC GAACAGTTCC AGCTCGCTGT GGCCAGACTC TCGCCAACGC
    CAACGACAAC GCCACCACAG TCCCTCCTCA ACCTCGATCT CGGCACTGAG CGGCGCTAAG
    AAGGCACGCA AATCCGCAAC CAACAGCAGC AGCAGCAATT CCACACAGCA GCAGCAGAAT
    CACCACGGCA CCAAAATCAA CAATCATCAT CTCAATGGCT ACGGCGATCA GTGGGGGGAG
    CAAAGCAGGA GTCGTCGCAA CTCTTCGCCC TCCAACAGTC ATAAAAATGA GAGAACCTCT
    GAGCGTCGCG TTCGTCCCAT CTACGACATC AATAACATCG TTATACCCTA CAGTATGCTA
    GCCCAGTCGA AAATGGAGAT AATTCCCTAC AAGGAAATAC CCATACCCAA GTGGCGCATT
    GTAGACAGTG ATAACGATAA GGGAAAACAT TCGTCAGATG AGTCAATGGA GCGCAAACTG
    AGCAATGGCT GTGTCGCATC AAAGTCAGAG GAGCTAGCCA AACAACAGCT ACCACCACCA
    CCAGATCAAG ATCAAGAGCA GCCGGCCCTG CAGCCTCCGA AGGTTAATGG TTTAAAGGAA
    AATCAAGCAG TCAAGCACAA TAACAATAAT AATACAAACA ATAATAATAA TAAAAATGGA
    CTGGTAAATG GTAACGCCAA AAAGGATGAA CTTAAGGCTG CAGAGGATCA CGATACGCAA
    TCAAAAGAAG ACGACGATCC AAAGGAAAAG GTGGTCAAAC CCAAGCTCAA CGGAAACTTG
    ATAAAGAATC CGATTGACAA AACTGCCGAA ACGCAAGACA AAATTGCTCA GCCAGCACCA
    GAACCACCAC TGCCAAAGCG ACCCAAGCTG GAAACTCCAA CCTCCAACGA GGTCACCAAG
    GCTAATGGGC AGTTGGTGCA GCAACAGCTG GAATCCCGTG AGAGCGAGGA GGAGCCACAG
    CATGTCGACG AAGATCTTTC GGATGAAGCG TTTATTATGC GACATCAGCG AGCTCTTCTC
    GAGGAGCGAC GCCGCTTCGA GACTTTCCTT AAGTTCCCCT GGAGCACCCG ATCGCGTGCA
    AATAGGCGCG TCGATAGTCG TGCCGAATCT AGTGGCGCCA ACACACCGGA TCCCGCCTCA
    CCAGCTCCTC ATCTAGGCGG CCCTGGTCAC GACAACGAAA GCATTCCCTC GCCGCTGGCG
    TACCCACTGG ACGCTTTTAA CGAAAGCGGT GAGCTGCTGA CTGGCGGACA GGCACGCCAA
    GCTCGTCGTC GGACTACGTC TAGCAAGCTT AAGGATCAGC TGGAACGGCG CAGCACCACG
    CCCGATCTGC GGGAGTCTTA CACTGTGATG CCATCTCCCT TTGAGCCCTT GAACTTTCCG
    CTCTCCGACG AGGTCTACCA GCGATTGCTA GCCGAGACAT ATGCGCCGCC CAGGTCAATG
    ACCGTCTCAA AGCGGGCCAA AAGCAAGTCG ATATCCTCGA ACTGTGATGG TGGGAACACC
    TTCACAGGAG GCAGCGGCAG TCGGCGCAGC AGCAAGTGCA AGATTAAGCC AAATGGTCAG
    CTAAATGGCC AGCTCAATGG CCACCCTGCG GCGGCGAAGT TGAACGGAAT AGATGCGGCA
    AATGAAGGTG GAATTTCTGA GCCGGAAGAG GAAGAGATGC TGCTAGAAGA CGAGGATGAC
    GACTATCCCA AGCATCATCT GGCGCCGCTG GATGACGAGG AGCCGTCGAC ACCAGATGTC
    GAGCATGAGC TTTACGACTC GGCGATCGAT GCCTATCTCG CTGATCCGGA GGCGCTAGAG
    GAGGATATGG GCGAGGATCC CTTCGAGGAC GACGATCCCA ACGATCCGGA GTGGAAGACA
    CGGACCGAGG GTGTTCGCAG CAGACGCATC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_732070.2
    CDS145..4797
    Translation

    Target ORF information:

    RefSeq Version NM_169673.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169673.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG CGGCCAGGGG CAAGCGGCAA
    CCACAACACC CAACTGGAGC GAACAGTTCC AGCTCGCTGT GGCCAGACTC TCGCCAACGC
    CAACGACAAC GCCACCACAG TCCCTCCTCA ACCTCGATCT CGGCACTGAG CGGCGCTAAG
    AAGGCACGCA AATCCGCAAC CAACAGCAGC AGCAGCAATT CCACACAGCA GCAGCAGAAT
    CACCACGGCA CCAAAATCAA CAATCATCAT CTCAATGGCT ACGGCGATCA GTGGGGGGAG
    CAAAGCAGGA GTCGTCGCAA CTCTTCGCCC TCCAACAGTC ATAAAAATGA GAGAACCTCT
    GAGCGTCGCG TTCGTCCCAT CTACGACATC AATAACATCG TTATACCCTA CAGTATGCTA
    GCCCAGTCGA AAATGGAGAT AATTCCCTAC AAGGAAATAC CCATACCCAA GTGGCGCATT
    GTAGACAGTG ATAACGATAA GGGAAAACAT TCGTCAGATG AGTCAATGGA GCGCAAACTG
    AGCAATGGCT GTGTCGCATC AAAGTCAGAG GAGCTAGCCA AACAACAGCT ACCACCACCA
    CCAGATCAAG ATCAAGAGCA GCCGGCCCTG CAGCCTCCGA AGGTTAATGG TTTAAAGGAA
    AATCAAGCAG TCAAGCACAA TAACAATAAT AATACAAACA ATAATAATAA TAAAAATGGA
    CTGGTAAATG GTAACGCCAA AAAGGATGAA CTTAAGGCTG CAGAGGATCA CGATACGCAA
    TCAAAAGAAG ACGACGATCC AAAGGAAAAG GTGGTCAAAC CCAAGCTCAA CGGAAACTTG
    ATAAAGAATC CGATTGACAA AACTGCCGAA ACGCAAGACA AAATTGCTCA GCCAGCACCA
    GAACCACCAC TGCCAAAGCG ACCCAAGCTG GAAACTCCAA CCTCCAACGA GGTCACCAAG
    GCTAATGGGC AGTTGGTGCA GCAACAGCTG GAATCCCGTG AGAGCGAGGA GGAGCCACAG
    CATGTCGACG AAGATCTTTC GGATGAAGCG TTTATTATGC GACATCAGCG AGCTCTTCTC
    GAGGAGCGAC GCCGCTTCGA GACTTTCCTT AAGTTCCCCT GGAGCACCCG ATCGCGTGCA
    AATAGGCGCG TCGATAGTCG TGCCGAATCT AGTGGCGCCA ACACACCGGA TCCCGCCTCA
    CCAGCTCCTC ATCTAGGCGG CCCTGGTCAC GACAACGAAA GCATTCCCTC GCCGCTGGCG
    TACCCACTGG ACGCTTTTAA CGAAAGCGGT GAGCTGCTGA CTGGCGGACA GGCACGCCAA
    GCTCGTCGTC GGACTACGTC TAGCAAGCTT AAGGATCAGC TGGAACGGCG CAGCACCACG
    CCCGATCTGC GGGAGTCTTA CACTGTGATG CCATCTCCCT TTGAGCCCTT GAACTTTCCG
    CTCTCCGACG AGGTCTACCA GCGATTGCTA GCCGAGACAT ATGCGCCGCC CAGGTCAATG
    ACCGTCTCAA AGCGGGCCAA AAGCAAGTCG ATATCCTCGA ACTGTGATGG TGGGAACACC
    TTCACAGGAG GCAGCGGCAG TCGGCGCAGC AGCAAGTGCA AGATTAAGCC AAATGGTCAG
    CTAAATGGCC AGCTCAATGG CCACCCTGCG GCGGCGAAGT TGAACGGAAT AGATGCGGCA
    AATGAAGGTG GAATTTCTGA GCCGGAAGAG GAAGAGATGC TGCTAGAAGA CGAGGATGAC
    GACTATCCCA AGCATCATCT GGCGCCGCTG GATGACGAGG AGCCGTCGAC ACCAGATGTC
    GAGCATGAGC TTTACGACTC GGCGATCGAT GCCTATCTCG CTGATCCGGA GGCGCTAGAG
    GAGGATATGG GCGAGGATCC CTTCGAGGAC GACGATCCCA ACGATCCGGA GTGGAAGACA
    CGGACCGAGG GTGTTCGCAG CAGACGCATC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05038
    Clone ID Related Accession (Same CDS sequence) NM_169672.4
    Accession Version NM_169672.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4713bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product non-specific lethal 1, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169672.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG GTACTGCTAC TACTTCTTCT
    TCTACTACGC CTACTACAAC GCCACTTGTG GCCAACACAG CGGCCAGGGG CAAGCGGCAA
    CCACAACACC CAACTGGAGC GAACAGTTCC AGCTCGCTGT GGCCAGACTC TCGCCAACGC
    CAACGACAAC GCCACCACAG TCCCTCCTCA ACCTCGATCT CGGCACTGAG CGGCGCTAAG
    AAGGCACGCA AATCCGCAAC CAACAGCAGC AGCAGCAATT CCACACAGCA GCAGCAGAAT
    CACCACGGCA CCAAAATCAA CAATCATCAT CTCAATGGCT ACGGCGATCA GTGGGGGGAG
    CAAAGCAGGA GTCGTCGCAA CTCTTCGCCC TCCAACAGTC ATAAAAATGA GAGAACCTCT
    GAGCGTCGCG TTCGTCCCAT CTACGACATC AATAACATCG TTATACCCTA CAGTATGCTA
    GCCCAGTCGA AAATGGAGAT AATTCCCTAC AAGGAAATAC CCATACCCAA GTGGCGCATT
    GTAGACAGTG ATAACGATAA GGGAAAACAT TCGTCAGATG AGTCAATGGA GCGCAAACTG
    AGCAATGGCT GTGTCGCATC AAAGTCAGAG GAGCTAGCCA AACAACAGCT ACCACCACCA
    CCAGATCAAG ATCAAGAGCA GCCGGCCCTG CAGCCTCCGA AGGTTAATGG TTTAAAGGAA
    AATCAAGCAG TCAAGCACAA TAACAATAAT AATACAAACA ATAATAATAA TAAAAATGGA
    CTGGTAAATG GTAACGCCAA AAAGGATGAA CTTAAGGCTG CAGAGGATCA CGATACGCAA
    TCAAAAGAAG ACGACGATCC AAAGGAAAAG GTGGTCAAAC CCAAGCTCAA CGGAAACTTG
    ATAAAGAATC CGATTGACAA AACTGCCGAA ACGCAAGACA AAATTGCTCA GCCAGCACCA
    GAACCACCAC TGCCAAAGCG ACCCAAGCTG GAAACTCCAA CCTCCAACGA GGTCACCAAG
    GCTAATGGGC AGTTGGTGCA GCAACAGCTG GAATCCCGTG AGAGCGAGGA GGAGCCACAG
    CATGTCGACG AAGATCTTTC GGATGAAGCG TTTATTATGC GACATCAGCG AGCTCTTCTC
    GAGGAGCGAC GCCGCTTCGA GACTTTCCTT AAGTTCCCCT GGAGCACCCG ATCGCGTGCA
    AATAGGCGCG TCGATAGTCG TGCCGAATCT AGTGGCGCCA ACACACCGGA TCCCGCCTCA
    CCAGCTCCTC ATCTAGGCGG CCCTGGTCAC GACAACGAAA GCATTCCCTC GCCGCTGGCG
    TACCCACTGG ACGCTTTTAA CGAAAGCGGT GAGCTGCTGA CTGGCGGACA GGCACGCCAA
    GCTCGTCGTC GGACTACGTC TAGCAAGCTT AAGGATCAGC TGGAACGGCG CAGCACCACG
    CCCGATCTGC GGGAGTCTTA CACTGTGATG CCATCTCCCT TTGAGCCCTT GAACTTTCCG
    CTCTCCGACG AGGTCTACCA GCGATTGCTA GCCGAGACAT ATGCGCCGCC CAGGTCAATG
    ACCGTCTCAA AGCGGGCCAA AAGCAAGTCG ATATCCTCGA ACTGTGATGG TGGGAACACC
    TTCACAGGAG GCAGCGGCAG TCGGCGCAGC AGCAAGTGCA AGATTAAGCC AAATGGTCAG
    CTAAATGGCC AGCTCAATGG CCACCCTGCG GCGGCGAAGT TGAACGGAAT AGATGCGGCA
    AATGAAGGTG GAATTTCTGA GCCGGAAGAG GAAGAGATGC TGCTAGAAGA CGAGGATGAC
    GACTATCCCA AGCATCATCT GGCGCCGCTG GATGACGAGG AGCCGTCGAC ACCAGATGTC
    GAGCATGAGC TTTACGACTC GGCGATCGAT GCCTATCTCG CTGATCCGGA GGCGCTAGAG
    GAGGATATGG GCGAGGATCC CTTCGAGGAC GACGATCCCA ACGATCCGGA GTGGAAGACA
    CGGACCGAGG GTGTTCGCAG CAGACGCATC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_732069.3
    CDS351..5063
    Translation

    Target ORF information:

    RefSeq Version NM_169672.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant M, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169672.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG GTACTGCTAC TACTTCTTCT
    TCTACTACGC CTACTACAAC GCCACTTGTG GCCAACACAG CGGCCAGGGG CAAGCGGCAA
    CCACAACACC CAACTGGAGC GAACAGTTCC AGCTCGCTGT GGCCAGACTC TCGCCAACGC
    CAACGACAAC GCCACCACAG TCCCTCCTCA ACCTCGATCT CGGCACTGAG CGGCGCTAAG
    AAGGCACGCA AATCCGCAAC CAACAGCAGC AGCAGCAATT CCACACAGCA GCAGCAGAAT
    CACCACGGCA CCAAAATCAA CAATCATCAT CTCAATGGCT ACGGCGATCA GTGGGGGGAG
    CAAAGCAGGA GTCGTCGCAA CTCTTCGCCC TCCAACAGTC ATAAAAATGA GAGAACCTCT
    GAGCGTCGCG TTCGTCCCAT CTACGACATC AATAACATCG TTATACCCTA CAGTATGCTA
    GCCCAGTCGA AAATGGAGAT AATTCCCTAC AAGGAAATAC CCATACCCAA GTGGCGCATT
    GTAGACAGTG ATAACGATAA GGGAAAACAT TCGTCAGATG AGTCAATGGA GCGCAAACTG
    AGCAATGGCT GTGTCGCATC AAAGTCAGAG GAGCTAGCCA AACAACAGCT ACCACCACCA
    CCAGATCAAG ATCAAGAGCA GCCGGCCCTG CAGCCTCCGA AGGTTAATGG TTTAAAGGAA
    AATCAAGCAG TCAAGCACAA TAACAATAAT AATACAAACA ATAATAATAA TAAAAATGGA
    CTGGTAAATG GTAACGCCAA AAAGGATGAA CTTAAGGCTG CAGAGGATCA CGATACGCAA
    TCAAAAGAAG ACGACGATCC AAAGGAAAAG GTGGTCAAAC CCAAGCTCAA CGGAAACTTG
    ATAAAGAATC CGATTGACAA AACTGCCGAA ACGCAAGACA AAATTGCTCA GCCAGCACCA
    GAACCACCAC TGCCAAAGCG ACCCAAGCTG GAAACTCCAA CCTCCAACGA GGTCACCAAG
    GCTAATGGGC AGTTGGTGCA GCAACAGCTG GAATCCCGTG AGAGCGAGGA GGAGCCACAG
    CATGTCGACG AAGATCTTTC GGATGAAGCG TTTATTATGC GACATCAGCG AGCTCTTCTC
    GAGGAGCGAC GCCGCTTCGA GACTTTCCTT AAGTTCCCCT GGAGCACCCG ATCGCGTGCA
    AATAGGCGCG TCGATAGTCG TGCCGAATCT AGTGGCGCCA ACACACCGGA TCCCGCCTCA
    CCAGCTCCTC ATCTAGGCGG CCCTGGTCAC GACAACGAAA GCATTCCCTC GCCGCTGGCG
    TACCCACTGG ACGCTTTTAA CGAAAGCGGT GAGCTGCTGA CTGGCGGACA GGCACGCCAA
    GCTCGTCGTC GGACTACGTC TAGCAAGCTT AAGGATCAGC TGGAACGGCG CAGCACCACG
    CCCGATCTGC GGGAGTCTTA CACTGTGATG CCATCTCCCT TTGAGCCCTT GAACTTTCCG
    CTCTCCGACG AGGTCTACCA GCGATTGCTA GCCGAGACAT ATGCGCCGCC CAGGTCAATG
    ACCGTCTCAA AGCGGGCCAA AAGCAAGTCG ATATCCTCGA ACTGTGATGG TGGGAACACC
    TTCACAGGAG GCAGCGGCAG TCGGCGCAGC AGCAAGTGCA AGATTAAGCC AAATGGTCAG
    CTAAATGGCC AGCTCAATGG CCACCCTGCG GCGGCGAAGT TGAACGGAAT AGATGCGGCA
    AATGAAGGTG GAATTTCTGA GCCGGAAGAG GAAGAGATGC TGCTAGAAGA CGAGGATGAC
    GACTATCCCA AGCATCATCT GGCGCCGCTG GATGACGAGG AGCCGTCGAC ACCAGATGTC
    GAGCATGAGC TTTACGACTC GGCGATCGAT GCCTATCTCG CTGATCCGGA GGCGCTAGAG
    GAGGATATGG GCGAGGATCC CTTCGAGGAC GACGATCCCA ACGATCCGGA GTGGAAGACA
    CGGACCGAGG GTGTTCGCAG CAGACGCATC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08406
    Clone ID Related Accession (Same CDS sequence) NM_001275684.1 , NM_001170151.3
    Accession Version NM_001275684.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product non-specific lethal 1, isoform P
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGCAGCGG CCAGGGGCAA GCGGCAACCA
    CAACACCCAA CTGGAGCGAA CAGTTCCAGC TCGCTGTGGC CAGACTCTCG CCAACGCCAA
    CGACAACGCC ACCACAGTCC CTCCTCAACC TCGATCTCGG CACTGAGCGG CGCTAAGAAG
    GCACGCAAAT CCGCAACCAA CAGCAGCAGC AGCAATTCCA CACAGCAGCA GCAGAATCAC
    CACGGCACCA AAATCAACAA TCATCATCTC AATGGCTACG GCGATCAGTG GGGGGAGCAA
    AGCAGGAGTC GTCGCAACTC TTCGCCCTCC AACAGTCATA AAAATGAGAG AACCTCTGAG
    CGTCGCGTTC GTCCCATCTA CGACATCAAT AACATCGTTA TACCCTACAG TATGCTAGCC
    CAGTCGAAAA TGGAGATAAT TCCCTACAAG GAAATACCCA TACCCAAGTG GCGCATTGTA
    GACAGTGATA ACGATAAGGG AAAACATTCG TCAGATGAGT CAATGGAGCG CAAACTGAGC
    AATGGCTGTG TCGCATCAAA GTCAGAGGAG CTAGCCAAAC AACAGCTACC ACCACCACCA
    GATCAAGATC AAGAGCAGCC GGCCCTGCAG CCTCCGAAGG TTAATGGTTT AAAGGAAAAT
    CAAGCAGTCA AGCACAATAA CAATAATAAT ACAAACAATA ATAATAATAA AAATGGACTG
    GTAAATGGTA ACGCCAAAAA GGATGAACTT AAGGCTGCAG AGGATCACGA TACGCAATCA
    AAAGAAGACG ACGATCCAAA GGAAAAGGTG GTCAAACCCA AGCTCAACGG AAACTTGATA
    AAGAATCCGA TTGACAAAAC TGCCGAAACG CAAGACAAAA TTGCTCAGCC AGCACCAGAA
    CCACCACTGC CAAAGCGACC CAAGCTGGAA ACTCCAACCT CCAACGAGGT CACCAAGGCT
    AATGGGCAGT TGGTGCAGCA ACAGCTGGAA TCCCGTGAGA GCGAGGAGGA GCCACAGCAT
    GTCGACGAAG ATCTTTCGGA TGAAGCGTTT ATTATGCGAC ATCAGCGAGC TCTTCTCGAG
    GAGCGACGCC GCTTCGAGAC TTTCCTTAAG TTCCCCTGGA GCACCCGATC GCGTGCAAAT
    AGGCGCGTCG ATAGTCGTGC CGAATCTAGT GGCGCCAACA CACCGGATCC CGCCTCACCA
    GCTCCTCATC TAGGCGGCCC TGGTCACGAC AACGAAAGCA TTCCCTCGCC GCTGGCGTAC
    CCACTGGACG CTTTTAACGA AAGCGGTGAG CTGCTGACTG GCGGACAGGC ACGCCAAGCT
    CGTCGTCGGA CTACGTCTAG CAAGCTTAAG GATCAGCTGG AACGGCGCAG CACCACGCCC
    GATCTGCGGG AGTCTTACAC TGTGATGCCA TCTCCCTTTG AGCCCTTGAA CTTTCCGCTC
    TCCGACGAGG TCTACCAGCG ATTGCTAGCC GAGACATATG CGCCGCCCAG GTCAATGACC
    GTCTCAAAGC GGGCCAAAAG CAAGTCGATA TCCTCGAACT GTGATGGTGG GAACACCTTC
    ACAGGAGGCA GCGGCAGTCG GCGCAGCAGC AAGTGCAAGA TTAAGCCAAA TGGTCAGCTA
    AATGGCCAGC TCAATGGCCA CCCTGCGGCG GCGAAGTTGA ACGGAATAGA TGCGGCAAAT
    GAAGGTGGAA TTTCTGAGCC GGAAGAGGAA GAGATGCTGC TAGAAGACGA GGATGACGAC
    TATCCCAAGC ATCATCTGGC GCCGCTGGAT GACGAGGAGC CGTCGACACC AGATGTCGAG
    CATGAGCTTT ACGACTCGGC GATCGATGCC TATCTCGCTG ATCCGGAGGC GCTAGAGGAG
    GATATGGGCG AGGATCCCTT CGAGGACGAC GATCCCAACG ATCCGGAGTG GAAGACACGG
    ACCGAGGGTG TTCGCAGCAG ACGCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262613.1
    CDS251..4900
    Translation

    Target ORF information:

    RefSeq Version NM_001275684.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant P, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001275684.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGCAGCGG CCAGGGGCAA GCGGCAACCA
    CAACACCCAA CTGGAGCGAA CAGTTCCAGC TCGCTGTGGC CAGACTCTCG CCAACGCCAA
    CGACAACGCC ACCACAGTCC CTCCTCAACC TCGATCTCGG CACTGAGCGG CGCTAAGAAG
    GCACGCAAAT CCGCAACCAA CAGCAGCAGC AGCAATTCCA CACAGCAGCA GCAGAATCAC
    CACGGCACCA AAATCAACAA TCATCATCTC AATGGCTACG GCGATCAGTG GGGGGAGCAA
    AGCAGGAGTC GTCGCAACTC TTCGCCCTCC AACAGTCATA AAAATGAGAG AACCTCTGAG
    CGTCGCGTTC GTCCCATCTA CGACATCAAT AACATCGTTA TACCCTACAG TATGCTAGCC
    CAGTCGAAAA TGGAGATAAT TCCCTACAAG GAAATACCCA TACCCAAGTG GCGCATTGTA
    GACAGTGATA ACGATAAGGG AAAACATTCG TCAGATGAGT CAATGGAGCG CAAACTGAGC
    AATGGCTGTG TCGCATCAAA GTCAGAGGAG CTAGCCAAAC AACAGCTACC ACCACCACCA
    GATCAAGATC AAGAGCAGCC GGCCCTGCAG CCTCCGAAGG TTAATGGTTT AAAGGAAAAT
    CAAGCAGTCA AGCACAATAA CAATAATAAT ACAAACAATA ATAATAATAA AAATGGACTG
    GTAAATGGTA ACGCCAAAAA GGATGAACTT AAGGCTGCAG AGGATCACGA TACGCAATCA
    AAAGAAGACG ACGATCCAAA GGAAAAGGTG GTCAAACCCA AGCTCAACGG AAACTTGATA
    AAGAATCCGA TTGACAAAAC TGCCGAAACG CAAGACAAAA TTGCTCAGCC AGCACCAGAA
    CCACCACTGC CAAAGCGACC CAAGCTGGAA ACTCCAACCT CCAACGAGGT CACCAAGGCT
    AATGGGCAGT TGGTGCAGCA ACAGCTGGAA TCCCGTGAGA GCGAGGAGGA GCCACAGCAT
    GTCGACGAAG ATCTTTCGGA TGAAGCGTTT ATTATGCGAC ATCAGCGAGC TCTTCTCGAG
    GAGCGACGCC GCTTCGAGAC TTTCCTTAAG TTCCCCTGGA GCACCCGATC GCGTGCAAAT
    AGGCGCGTCG ATAGTCGTGC CGAATCTAGT GGCGCCAACA CACCGGATCC CGCCTCACCA
    GCTCCTCATC TAGGCGGCCC TGGTCACGAC AACGAAAGCA TTCCCTCGCC GCTGGCGTAC
    CCACTGGACG CTTTTAACGA AAGCGGTGAG CTGCTGACTG GCGGACAGGC ACGCCAAGCT
    CGTCGTCGGA CTACGTCTAG CAAGCTTAAG GATCAGCTGG AACGGCGCAG CACCACGCCC
    GATCTGCGGG AGTCTTACAC TGTGATGCCA TCTCCCTTTG AGCCCTTGAA CTTTCCGCTC
    TCCGACGAGG TCTACCAGCG ATTGCTAGCC GAGACATATG CGCCGCCCAG GTCAATGACC
    GTCTCAAAGC GGGCCAAAAG CAAGTCGATA TCCTCGAACT GTGATGGTGG GAACACCTTC
    ACAGGAGGCA GCGGCAGTCG GCGCAGCAGC AAGTGCAAGA TTAAGCCAAA TGGTCAGCTA
    AATGGCCAGC TCAATGGCCA CCCTGCGGCG GCGAAGTTGA ACGGAATAGA TGCGGCAAAT
    GAAGGTGGAA TTTCTGAGCC GGAAGAGGAA GAGATGCTGC TAGAAGACGA GGATGACGAC
    TATCCCAAGC ATCATCTGGC GCCGCTGGAT GACGAGGAGC CGTCGACACC AGATGTCGAG
    CATGAGCTTT ACGACTCGGC GATCGATGCC TATCTCGCTG ATCCGGAGGC GCTAGAGGAG
    GATATGGGCG AGGATCCCTT CGAGGACGAC GATCCCAACG ATCCGGAGTG GAAGACACGG
    ACCGAGGGTG TTCGCAGCAG ACGCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08406
    Clone ID Related Accession (Same CDS sequence) NM_001275684.1 , NM_001170151.3
    Accession Version NM_001170151.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product non-specific lethal 1, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170151.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGCAGCGG CCAGGGGCAA GCGGCAACCA
    CAACACCCAA CTGGAGCGAA CAGTTCCAGC TCGCTGTGGC CAGACTCTCG CCAACGCCAA
    CGACAACGCC ACCACAGTCC CTCCTCAACC TCGATCTCGG CACTGAGCGG CGCTAAGAAG
    GCACGCAAAT CCGCAACCAA CAGCAGCAGC AGCAATTCCA CACAGCAGCA GCAGAATCAC
    CACGGCACCA AAATCAACAA TCATCATCTC AATGGCTACG GCGATCAGTG GGGGGAGCAA
    AGCAGGAGTC GTCGCAACTC TTCGCCCTCC AACAGTCATA AAAATGAGAG AACCTCTGAG
    CGTCGCGTTC GTCCCATCTA CGACATCAAT AACATCGTTA TACCCTACAG TATGCTAGCC
    CAGTCGAAAA TGGAGATAAT TCCCTACAAG GAAATACCCA TACCCAAGTG GCGCATTGTA
    GACAGTGATA ACGATAAGGG AAAACATTCG TCAGATGAGT CAATGGAGCG CAAACTGAGC
    AATGGCTGTG TCGCATCAAA GTCAGAGGAG CTAGCCAAAC AACAGCTACC ACCACCACCA
    GATCAAGATC AAGAGCAGCC GGCCCTGCAG CCTCCGAAGG TTAATGGTTT AAAGGAAAAT
    CAAGCAGTCA AGCACAATAA CAATAATAAT ACAAACAATA ATAATAATAA AAATGGACTG
    GTAAATGGTA ACGCCAAAAA GGATGAACTT AAGGCTGCAG AGGATCACGA TACGCAATCA
    AAAGAAGACG ACGATCCAAA GGAAAAGGTG GTCAAACCCA AGCTCAACGG AAACTTGATA
    AAGAATCCGA TTGACAAAAC TGCCGAAACG CAAGACAAAA TTGCTCAGCC AGCACCAGAA
    CCACCACTGC CAAAGCGACC CAAGCTGGAA ACTCCAACCT CCAACGAGGT CACCAAGGCT
    AATGGGCAGT TGGTGCAGCA ACAGCTGGAA TCCCGTGAGA GCGAGGAGGA GCCACAGCAT
    GTCGACGAAG ATCTTTCGGA TGAAGCGTTT ATTATGCGAC ATCAGCGAGC TCTTCTCGAG
    GAGCGACGCC GCTTCGAGAC TTTCCTTAAG TTCCCCTGGA GCACCCGATC GCGTGCAAAT
    AGGCGCGTCG ATAGTCGTGC CGAATCTAGT GGCGCCAACA CACCGGATCC CGCCTCACCA
    GCTCCTCATC TAGGCGGCCC TGGTCACGAC AACGAAAGCA TTCCCTCGCC GCTGGCGTAC
    CCACTGGACG CTTTTAACGA AAGCGGTGAG CTGCTGACTG GCGGACAGGC ACGCCAAGCT
    CGTCGTCGGA CTACGTCTAG CAAGCTTAAG GATCAGCTGG AACGGCGCAG CACCACGCCC
    GATCTGCGGG AGTCTTACAC TGTGATGCCA TCTCCCTTTG AGCCCTTGAA CTTTCCGCTC
    TCCGACGAGG TCTACCAGCG ATTGCTAGCC GAGACATATG CGCCGCCCAG GTCAATGACC
    GTCTCAAAGC GGGCCAAAAG CAAGTCGATA TCCTCGAACT GTGATGGTGG GAACACCTTC
    ACAGGAGGCA GCGGCAGTCG GCGCAGCAGC AAGTGCAAGA TTAAGCCAAA TGGTCAGCTA
    AATGGCCAGC TCAATGGCCA CCCTGCGGCG GCGAAGTTGA ACGGAATAGA TGCGGCAAAT
    GAAGGTGGAA TTTCTGAGCC GGAAGAGGAA GAGATGCTGC TAGAAGACGA GGATGACGAC
    TATCCCAAGC ATCATCTGGC GCCGCTGGAT GACGAGGAGC CGTCGACACC AGATGTCGAG
    CATGAGCTTT ACGACTCGGC GATCGATGCC TATCTCGCTG ATCCGGAGGC GCTAGAGGAG
    GATATGGGCG AGGATCCCTT CGAGGACGAC GATCCCAACG ATCCGGAGTG GAAGACACGG
    ACCGAGGGTG TTCGCAGCAG ACGCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163622.1
    CDS498..5147
    Translation

    Target ORF information:

    RefSeq Version NM_001170151.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170151.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGCAGCGG CCAGGGGCAA GCGGCAACCA
    CAACACCCAA CTGGAGCGAA CAGTTCCAGC TCGCTGTGGC CAGACTCTCG CCAACGCCAA
    CGACAACGCC ACCACAGTCC CTCCTCAACC TCGATCTCGG CACTGAGCGG CGCTAAGAAG
    GCACGCAAAT CCGCAACCAA CAGCAGCAGC AGCAATTCCA CACAGCAGCA GCAGAATCAC
    CACGGCACCA AAATCAACAA TCATCATCTC AATGGCTACG GCGATCAGTG GGGGGAGCAA
    AGCAGGAGTC GTCGCAACTC TTCGCCCTCC AACAGTCATA AAAATGAGAG AACCTCTGAG
    CGTCGCGTTC GTCCCATCTA CGACATCAAT AACATCGTTA TACCCTACAG TATGCTAGCC
    CAGTCGAAAA TGGAGATAAT TCCCTACAAG GAAATACCCA TACCCAAGTG GCGCATTGTA
    GACAGTGATA ACGATAAGGG AAAACATTCG TCAGATGAGT CAATGGAGCG CAAACTGAGC
    AATGGCTGTG TCGCATCAAA GTCAGAGGAG CTAGCCAAAC AACAGCTACC ACCACCACCA
    GATCAAGATC AAGAGCAGCC GGCCCTGCAG CCTCCGAAGG TTAATGGTTT AAAGGAAAAT
    CAAGCAGTCA AGCACAATAA CAATAATAAT ACAAACAATA ATAATAATAA AAATGGACTG
    GTAAATGGTA ACGCCAAAAA GGATGAACTT AAGGCTGCAG AGGATCACGA TACGCAATCA
    AAAGAAGACG ACGATCCAAA GGAAAAGGTG GTCAAACCCA AGCTCAACGG AAACTTGATA
    AAGAATCCGA TTGACAAAAC TGCCGAAACG CAAGACAAAA TTGCTCAGCC AGCACCAGAA
    CCACCACTGC CAAAGCGACC CAAGCTGGAA ACTCCAACCT CCAACGAGGT CACCAAGGCT
    AATGGGCAGT TGGTGCAGCA ACAGCTGGAA TCCCGTGAGA GCGAGGAGGA GCCACAGCAT
    GTCGACGAAG ATCTTTCGGA TGAAGCGTTT ATTATGCGAC ATCAGCGAGC TCTTCTCGAG
    GAGCGACGCC GCTTCGAGAC TTTCCTTAAG TTCCCCTGGA GCACCCGATC GCGTGCAAAT
    AGGCGCGTCG ATAGTCGTGC CGAATCTAGT GGCGCCAACA CACCGGATCC CGCCTCACCA
    GCTCCTCATC TAGGCGGCCC TGGTCACGAC AACGAAAGCA TTCCCTCGCC GCTGGCGTAC
    CCACTGGACG CTTTTAACGA AAGCGGTGAG CTGCTGACTG GCGGACAGGC ACGCCAAGCT
    CGTCGTCGGA CTACGTCTAG CAAGCTTAAG GATCAGCTGG AACGGCGCAG CACCACGCCC
    GATCTGCGGG AGTCTTACAC TGTGATGCCA TCTCCCTTTG AGCCCTTGAA CTTTCCGCTC
    TCCGACGAGG TCTACCAGCG ATTGCTAGCC GAGACATATG CGCCGCCCAG GTCAATGACC
    GTCTCAAAGC GGGCCAAAAG CAAGTCGATA TCCTCGAACT GTGATGGTGG GAACACCTTC
    ACAGGAGGCA GCGGCAGTCG GCGCAGCAGC AAGTGCAAGA TTAAGCCAAA TGGTCAGCTA
    AATGGCCAGC TCAATGGCCA CCCTGCGGCG GCGAAGTTGA ACGGAATAGA TGCGGCAAAT
    GAAGGTGGAA TTTCTGAGCC GGAAGAGGAA GAGATGCTGC TAGAAGACGA GGATGACGAC
    TATCCCAAGC ATCATCTGGC GCCGCTGGAT GACGAGGAGC CGTCGACACC AGATGTCGAG
    CATGAGCTTT ACGACTCGGC GATCGATGCC TATCTCGCTG ATCCGGAGGC GCTAGAGGAG
    GATATGGGCG AGGATCCCTT CGAGGACGAC GATCCCAACG ATCCGGAGTG GAAGACACGG
    ACCGAGGGTG TTCGCAGCAG ACGCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08407
    Clone ID Related Accession (Same CDS sequence) NM_001260197.2
    Accession Version NM_001260197.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product non-specific lethal 1, isoform O
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260197.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG CAGCGGCCAG GGGCAAGCGG
    CAACCACAAC ACCCAACTGG AGCGAACAGT TCCAGCTCGC TGTGGCCAGA CTCTCGCCAA
    CGCCAACGAC AACGCCACCA CAGTCCCTCC TCAACCTCGA TCTCGGCACT GAGCGGCGCT
    AAGAAGGCAC GCAAATCCGC AACCAACAGC AGCAGCAGCA ATTCCACACA GCAGCAGCAG
    AATCACCACG GCACCAAAAT CAACAATCAT CATCTCAATG GCTACGGCGA TCAGTGGGGG
    GAGCAAAGCA GGAGTCGTCG CAACTCTTCG CCCTCCAACA GTCATAAAAA TGAGAGAACC
    TCTGAGCGTC GCGTTCGTCC CATCTACGAC ATCAATAACA TCGTTATACC CTACAGTATG
    CTAGCCCAGT CGAAAATGGA GATAATTCCC TACAAGGAAA TACCCATACC CAAGTGGCGC
    ATTGTAGACA GTGATAACGA TAAGGGAAAA CATTCGTCAG ATGAGTCAAT GGAGCGCAAA
    CTGAGCAATG GCTGTGTCGC ATCAAAGTCA GAGGAGCTAG CCAAACAACA GCTACCACCA
    CCACCAGATC AAGATCAAGA GCAGCCGGCC CTGCAGCCTC CGAAGGTTAA TGGTTTAAAG
    GAAAATCAAG CAGTCAAGCA CAATAACAAT AATAATACAA ACAATAATAA TAATAAAAAT
    GGACTGGTAA ATGGTAACGC CAAAAAGGAT GAACTTAAGG CTGCAGAGGA TCACGATACG
    CAATCAAAAG AAGACGACGA TCCAAAGGAA AAGGTGGTCA AACCCAAGCT CAACGGAAAC
    TTGATAAAGA ATCCGATTGA CAAAACTGCC GAAACGCAAG ACAAAATTGC TCAGCCAGCA
    CCAGAACCAC CACTGCCAAA GCGACCCAAG CTGGAAACTC CAACCTCCAA CGAGGTCACC
    AAGGCTAATG GGCAGTTGGT GCAGCAACAG CTGGAATCCC GTGAGAGCGA GGAGGAGCCA
    CAGCATGTCG ACGAAGATCT TTCGGATGAA GCGTTTATTA TGCGACATCA GCGAGCTCTT
    CTCGAGGAGC GACGCCGCTT CGAGACTTTC CTTAAGTTCC CCTGGAGCAC CCGATCGCGT
    GCAAATAGGC GCGTCGATAG TCGTGCCGAA TCTAGTGGCG CCAACACACC GGATCCCGCC
    TCACCAGCTC CTCATCTAGG CGGCCCTGGT CACGACAACG AAAGCATTCC CTCGCCGCTG
    GCGTACCCAC TGGACGCTTT TAACGAAAGC GGTGAGCTGC TGACTGGCGG ACAGGCACGC
    CAAGCTCGTC GTCGGACTAC GTCTAGCAAG CTTAAGGATC AGCTGGAACG GCGCAGCACC
    ACGCCCGATC TGCGGGAGTC TTACACTGTG ATGCCATCTC CCTTTGAGCC CTTGAACTTT
    CCGCTCTCCG ACGAGGTCTA CCAGCGATTG CTAGCCGAGA CATATGCGCC GCCCAGGTCA
    ATGACCGTCT CAAAGCGGGC CAAAAGCAAG TCGATATCCT CGAACTGTGA TGGTGGGAAC
    ACCTTCACAG GAGGCAGCGG CAGTCGGCGC AGCAGCAAGT GCAAGATTAA GCCAAATGGT
    CAGCTAAATG GCCAGCTCAA TGGCCACCCT GCGGCGGCGA AGTTGAACGG AATAGATGCG
    GCAAATGAAG GTGGAATTTC TGAGCCGGAA GAGGAAGAGA TGCTGCTAGA AGACGAGGAT
    GACGACTATC CCAAGCATCA TCTGGCGCCG CTGGATGACG AGGAGCCGTC GACACCAGAT
    GTCGAGCATG AGCTTTACGA CTCGGCGATC GATGCCTATC TCGCTGATCC GGAGGCGCTA
    GAGGAGGATA TGGGCGAGGA TCCCTTCGAG GACGACGATC CCAACGATCC GGAGTGGAAG
    ACACGGACCG AGGGTGTTCG CAGCAGACGC ATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247126.1
    CDS347..5002
    Translation

    Target ORF information:

    RefSeq Version NM_001260197.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant O, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001260197.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG CAGCGGCCAG GGGCAAGCGG
    CAACCACAAC ACCCAACTGG AGCGAACAGT TCCAGCTCGC TGTGGCCAGA CTCTCGCCAA
    CGCCAACGAC AACGCCACCA CAGTCCCTCC TCAACCTCGA TCTCGGCACT GAGCGGCGCT
    AAGAAGGCAC GCAAATCCGC AACCAACAGC AGCAGCAGCA ATTCCACACA GCAGCAGCAG
    AATCACCACG GCACCAAAAT CAACAATCAT CATCTCAATG GCTACGGCGA TCAGTGGGGG
    GAGCAAAGCA GGAGTCGTCG CAACTCTTCG CCCTCCAACA GTCATAAAAA TGAGAGAACC
    TCTGAGCGTC GCGTTCGTCC CATCTACGAC ATCAATAACA TCGTTATACC CTACAGTATG
    CTAGCCCAGT CGAAAATGGA GATAATTCCC TACAAGGAAA TACCCATACC CAAGTGGCGC
    ATTGTAGACA GTGATAACGA TAAGGGAAAA CATTCGTCAG ATGAGTCAAT GGAGCGCAAA
    CTGAGCAATG GCTGTGTCGC ATCAAAGTCA GAGGAGCTAG CCAAACAACA GCTACCACCA
    CCACCAGATC AAGATCAAGA GCAGCCGGCC CTGCAGCCTC CGAAGGTTAA TGGTTTAAAG
    GAAAATCAAG CAGTCAAGCA CAATAACAAT AATAATACAA ACAATAATAA TAATAAAAAT
    GGACTGGTAA ATGGTAACGC CAAAAAGGAT GAACTTAAGG CTGCAGAGGA TCACGATACG
    CAATCAAAAG AAGACGACGA TCCAAAGGAA AAGGTGGTCA AACCCAAGCT CAACGGAAAC
    TTGATAAAGA ATCCGATTGA CAAAACTGCC GAAACGCAAG ACAAAATTGC TCAGCCAGCA
    CCAGAACCAC CACTGCCAAA GCGACCCAAG CTGGAAACTC CAACCTCCAA CGAGGTCACC
    AAGGCTAATG GGCAGTTGGT GCAGCAACAG CTGGAATCCC GTGAGAGCGA GGAGGAGCCA
    CAGCATGTCG ACGAAGATCT TTCGGATGAA GCGTTTATTA TGCGACATCA GCGAGCTCTT
    CTCGAGGAGC GACGCCGCTT CGAGACTTTC CTTAAGTTCC CCTGGAGCAC CCGATCGCGT
    GCAAATAGGC GCGTCGATAG TCGTGCCGAA TCTAGTGGCG CCAACACACC GGATCCCGCC
    TCACCAGCTC CTCATCTAGG CGGCCCTGGT CACGACAACG AAAGCATTCC CTCGCCGCTG
    GCGTACCCAC TGGACGCTTT TAACGAAAGC GGTGAGCTGC TGACTGGCGG ACAGGCACGC
    CAAGCTCGTC GTCGGACTAC GTCTAGCAAG CTTAAGGATC AGCTGGAACG GCGCAGCACC
    ACGCCCGATC TGCGGGAGTC TTACACTGTG ATGCCATCTC CCTTTGAGCC CTTGAACTTT
    CCGCTCTCCG ACGAGGTCTA CCAGCGATTG CTAGCCGAGA CATATGCGCC GCCCAGGTCA
    ATGACCGTCT CAAAGCGGGC CAAAAGCAAG TCGATATCCT CGAACTGTGA TGGTGGGAAC
    ACCTTCACAG GAGGCAGCGG CAGTCGGCGC AGCAGCAAGT GCAAGATTAA GCCAAATGGT
    CAGCTAAATG GCCAGCTCAA TGGCCACCCT GCGGCGGCGA AGTTGAACGG AATAGATGCG
    GCAAATGAAG GTGGAATTTC TGAGCCGGAA GAGGAAGAGA TGCTGCTAGA AGACGAGGAT
    GACGACTATC CCAAGCATCA TCTGGCGCCG CTGGATGACG AGGAGCCGTC GACACCAGAT
    GTCGAGCATG AGCTTTACGA CTCGGCGATC GATGCCTATC TCGCTGATCC GGAGGCGCTA
    GAGGAGGATA TGGGCGAGGA TCCCTTCGAG GACGACGATC CCAACGATCC GGAGTGGAAG
    ACACGGACCG AGGGTGTTCG CAGCAGACGC ATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08411
    Clone ID Related Accession (Same CDS sequence) NM_001260196.2
    Accession Version NM_001260196.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product non-specific lethal 1, isoform N
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260196.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG GTACTGCTAC TACTTCTTCT
    TCTACTACGC CTACTACAAC GCCACTTGTG GCCAACACGG CCAGGGGCAA GCGGCAACCA
    CAACACCCAA CTGGAGCGAA CAGTTCCAGC TCGCTGTGGC CAGACTCTCG CCAACGCCAA
    CGACAACGCC ACCACAGTCC CTCCTCAACC TCGATCTCGG CACTGAGCGG CGCTAAGAAG
    GCACGCAAAT CCGCAACCAA CAGCAGCAGC AGCAATTCCA CACAGCAGCA GCAGAATCAC
    CACGGCACCA AAATCAACAA TCATCATCTC AATGGCTACG GCGATCAGTG GGGGGAGCAA
    AGCAGGAGTC GTCGCAACTC TTCGCCCTCC AACAGTCATA AAAATGAGAG AACCTCTGAG
    CGTCGCGTTC GTCCCATCTA CGACATCAAT AACATCGTTA TACCCTACAG TATGCTAGCC
    CAGTCGAAAA TGGAGATAAT TCCCTACAAG GAAATACCCA TACCCAAGTG GCGCATTGTA
    GACAGTGATA ACGATAAGGG AAAACATTCG TCAGATGAGT CAATGGAGCG CAAACTGAGC
    AATGGCTGTG TCGCATCAAA GTCAGAGGAG CTAGCCAAAC AACAGCTACC ACCACCACCA
    GATCAAGATC AAGAGCAGCC GGCCCTGCAG CCTCCGAAGG TTAATGGTTT AAAGGAAAAT
    CAAGCAGTCA AGCACAATAA CAATAATAAT ACAAACAATA ATAATAATAA AAATGGACTG
    GTAAATGGTA ACGCCAAAAA GGATGAACTT AAGGCTGCAG AGGATCACGA TACGCAATCA
    AAAGAAGACG ACGATCCAAA GGAAAAGGTG GTCAAACCCA AGCTCAACGG AAACTTGATA
    AAGAATCCGA TTGACAAAAC TGCCGAAACG CAAGACAAAA TTGCTCAGCC AGCACCAGAA
    CCACCACTGC CAAAGCGACC CAAGCTGGAA ACTCCAACCT CCAACGAGGT CACCAAGGCT
    AATGGGCAGT TGGTGCAGCA ACAGCTGGAA TCCCGTGAGA GCGAGGAGGA GCCACAGCAT
    GTCGACGAAG ATCTTTCGGA TGAAGCGTTT ATTATGCGAC ATCAGCGAGC TCTTCTCGAG
    GAGCGACGCC GCTTCGAGAC TTTCCTTAAG TTCCCCTGGA GCACCCGATC GCGTGCAAAT
    AGGCGCGTCG ATAGTCGTGC CGAATCTAGT GGCGCCAACA CACCGGATCC CGCCTCACCA
    GCTCCTCATC TAGGCGGCCC TGGTCACGAC AACGAAAGCA TTCCCTCGCC GCTGGCGTAC
    CCACTGGACG CTTTTAACGA AAGCGGTGAG CTGCTGACTG GCGGACAGGC ACGCCAAGCT
    CGTCGTCGGA CTACGTCTAG CAAGCTTAAG GATCAGCTGG AACGGCGCAG CACCACGCCC
    GATCTGCGGG AGTCTTACAC TGTGATGCCA TCTCCCTTTG AGCCCTTGAA CTTTCCGCTC
    TCCGACGAGG TCTACCAGCG ATTGCTAGCC GAGACATATG CGCCGCCCAG GTCAATGACC
    GTCTCAAAGC GGGCCAAAAG CAAGTCGATA TCCTCGAACT GTGATGGTGG GAACACCTTC
    ACAGGAGGCA GCGGCAGTCG GCGCAGCAGC AAGTGCAAGA TTAAGCCAAA TGGTCAGCTA
    AATGGCCAGC TCAATGGCCA CCCTGCGGCG GCGAAGTTGA ACGGAATAGA TGCGGCAAAT
    GAAGGTGGAA TTTCTGAGCC GGAAGAGGAA GAGATGCTGC TAGAAGACGA GGATGACGAC
    TATCCCAAGC ATCATCTGGC GCCGCTGGAT GACGAGGAGC CGTCGACACC AGATGTCGAG
    CATGAGCTTT ACGACTCGGC GATCGATGCC TATCTCGCTG ATCCGGAGGC GCTAGAGGAG
    GATATGGGCG AGGATCCCTT CGAGGACGAC GATCCCAACG ATCCGGAGTG GAAGACACGG
    ACCGAGGGTG TTCGCAGCAG ACGCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247125.1
    CDS251..4960
    Translation

    Target ORF information:

    RefSeq Version NM_001260196.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster non-specific lethal 1 (nsl1), transcript variant N, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001260196.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCCCCAG CGCTCACAGC CGAGCCACTA AGTCCAAAGG AGAAGCTAAC CAGCACCGCC 
    TCGCCCTCGG CCCGCAGCTC CTCGGAAAGC GGAGGAGTTG GATGTGCAGT TGGCTTGGTC
    AAGAAGCCGG CAACACCCAC GCCGGCCACG GCCACCACGC GGATTACCCG CTCATCAGCG
    GCAGCGGCCT CAGTTACCGC TTCCCCGCAT CTACAACAGC AGCGATCCTC GCTAGCAATC
    AATAACAAGC GAAAGTCGGG CAACGCTAAA GCCAGCAATG AACCAATGGG GCTGAGCACA
    GCCCCGACTC CACACAGCAC CACTTCACCA AATCCCTCGA CGGATACTGC GGCAGCCTCC
    ACCTCTGCCG TGGAGCACAA CAATAACGAC AGCAACAGTA CCTCCGCCGG CAACAGCACC
    TCCGCCGACA ACAGCTCCAC GCCCAAGGAC AACTCCACCG CTCAGCTACT GGCGGATCTA
    ACCATCAACT TTGAGGAGAC CATATCCGCG GAGATTTGCC TGAGGAAAAC CCTACCCGAT
    GTGAGTCTGG GGAAAGAGCC GGTTCCGCCT GCCTCGGTGC TGGCAGTGGC CACAAGTTCA
    ACGAGTGCTG GCAGCGCTCT ATCCTTATCT GCAGTGGATG AGGTGACCAA GATAGAAATA
    GATAATATAG AAGAGCGTCT CAGCCAGCTA GATGGGAATG CCACTGGAAG GCCCGAGGAG
    CATGTGGCAC CACCTTCTGT TCCGGTACCC GTGCAGGAGG CGCCAAACAC GCCCACCAGG
    CCACAGGCGC CACCAAGTGC CCATCTTGAG TCTCCGCAGC CGGCGCGAAC ACAGCAGCAG
    TCCATTCAAC AGATGACGCC GCAAAGCACC ACCAGTTCCA ACAATTTTCT CAAAGACGGA
    GCAGCTGGTG CTGTTCTAGA GGATCAGGAT ATCGAGGAGG TGCTTAAAGC GCTCAAAACA
    TTTGACGGTG GCCATGTTAA TCCAGATACC ATTTGCGAGT TTTTCGACGA AGTTTGGGAA
    GAGGCAGCAC CGGCAGCGCC ATTGCCGCCC GCGCCACCCG AAAATGTGGT GGATGTGCCG
    GGCGCGAAGC CCAGCTGCAG TGGCATCCTC GTTAGCGGCA GCAACATTTC CCAGCCGAAT
    GTGATTATTA AGCAGGAGCA GCGGCCCTGG CAGGAGAGTC ATGCTGAGTT GGAGCAGCAG
    CAGCACGTTA TCTCGCGCAG GATCGAGTTT TTGCTACGCA GGATGCGCAA GCTCCAGGCG
    CGAGCGATGT GCCGTCACGC GAGCGAGGAG GTGGTTGGTA TCATGGAGTG GTCTGCCCGC
    AGTTCCCATA AGGCTCCAGT TCCAGCGAGA AGTTCCACGC TGACTGAGCA GGAGGCCACT
    GTCCTATCGA TTGTCTCAGG GCGTCCGAGC TCAACCTTCT GGGAGGAGCA GAACAAGCAT
    CCGCTGCCTG CTAGCCAGAT GAGTAATGTG ATCCGGCATA TTGCGACGGC GGCAAAGCAT
    CAGCAAATCT GCCACTCAGC CAGCGGAAGC TCTGCTACTC TGGCGCCATC CTCCAGCTGG
    TACAACAGCT GCAACAGCTC TGCGCTGCCG ATGAAGCGTC CACGAAAGAA TCAACTTGAT
    GCTTCGGTTG CAACGGGTGC CACACCCACT GCACCTGCGC CAGGAGCGGT AACATCTAGA
    CAGGCAGCTG GCACGACGGG ATCCGCAACG GGTTCGAATA CACCGCGGGC GGATGATATC
    GTGCCCGGCT ACGACACCTA TGTGACTAAT GAACTCACCC ATGTGTCTGG CCTGCTGCAC
    ACGGAGCTTC GAGAAGTACA GAATGCCATC GACTCGGACG CCACGGAGTC AAGTTCTGGT
    GGAGAATCCG CTGACGAGAT GGTAACCTAC AACAACAACC AGCAGCTCTC GCTACCAATC
    ACTCGACGCG CTGTCTGGCG ATATTCGAGA GATCGTGCAG CCATTGCCCT GCGCTGGTCC
    TGGCTCTGCT CCCAGCTGAC CGATTTGGAG ATGAAGATCC GTCAGCACAG CGACCTTTAC
    ATGGATCTAA CTCAGTCCAA GGGCGAGATC CAGCTGGAGT CCACAGCTAA GACCTCACCG
    TCGCTAGCAC AGCCGGCAAA TGGCATTAAG GAGGAGCCCG GATCCGACTA CCTCTGCAGT
    CGGGCACGTC CATTGGTGCT GTCCGAGTTC CGCAAACGCA AGCTCTTCCA GACCACGAAC
    ATGCACACGA TTTCCAAGAA AGCCGCACGC CCCAGCAACA TTAAGTGTGG CTGCCAGTGG
    CCGCAGGTGC CATGCACACT GTGCACGGGT CGGACAGACC CTACGGCGCC CAGGGATCTG
    GTCGAGACGA TGATGCCGCA AAACCGGGTG GCATTGCTCG ATGCTGGCTA CCATCCAGTG
    CTCAGCTTCG CCAGCGATGT CAGTCAGTCG GTTCACTTGG AGGCCGTTGC CCGCCAGCCG
    GACTGGCAGT ACCGCGTCAT GCGCAGCCAG CCCAAGGCCA TTGTCAAGGC CATGTGGAAG
    GCGGAGCGCG AGACGATAGC CTCAGGCGGT GTCGGCGGAC AAGGTGGCAG CCGGCGGTCG
    GGCGATGCGG CGAAGCGGCG TTATATACGA CGCAAGGAGC GCAACAACAA CTCAAATAAG
    GAGGCTGGGA GCGGAGCTAA AGCTGCTGCT GTCGCCGCAG GAGGAGCAGG CGGAAACGGA
    AGCAGTAGCG TCGGGGGAGG GGATAGCGGA AACGGTACTG GTACTGCTAC TACTTCTTCT
    TCTACTACGC CTACTACAAC GCCACTTGTG GCCAACACGG CCAGGGGCAA GCGGCAACCA
    CAACACCCAA CTGGAGCGAA CAGTTCCAGC TCGCTGTGGC CAGACTCTCG CCAACGCCAA
    CGACAACGCC ACCACAGTCC CTCCTCAACC TCGATCTCGG CACTGAGCGG CGCTAAGAAG
    GCACGCAAAT CCGCAACCAA CAGCAGCAGC AGCAATTCCA CACAGCAGCA GCAGAATCAC
    CACGGCACCA AAATCAACAA TCATCATCTC AATGGCTACG GCGATCAGTG GGGGGAGCAA
    AGCAGGAGTC GTCGCAACTC TTCGCCCTCC AACAGTCATA AAAATGAGAG AACCTCTGAG
    CGTCGCGTTC GTCCCATCTA CGACATCAAT AACATCGTTA TACCCTACAG TATGCTAGCC
    CAGTCGAAAA TGGAGATAAT TCCCTACAAG GAAATACCCA TACCCAAGTG GCGCATTGTA
    GACAGTGATA ACGATAAGGG AAAACATTCG TCAGATGAGT CAATGGAGCG CAAACTGAGC
    AATGGCTGTG TCGCATCAAA GTCAGAGGAG CTAGCCAAAC AACAGCTACC ACCACCACCA
    GATCAAGATC AAGAGCAGCC GGCCCTGCAG CCTCCGAAGG TTAATGGTTT AAAGGAAAAT
    CAAGCAGTCA AGCACAATAA CAATAATAAT ACAAACAATA ATAATAATAA AAATGGACTG
    GTAAATGGTA ACGCCAAAAA GGATGAACTT AAGGCTGCAG AGGATCACGA TACGCAATCA
    AAAGAAGACG ACGATCCAAA GGAAAAGGTG GTCAAACCCA AGCTCAACGG AAACTTGATA
    AAGAATCCGA TTGACAAAAC TGCCGAAACG CAAGACAAAA TTGCTCAGCC AGCACCAGAA
    CCACCACTGC CAAAGCGACC CAAGCTGGAA ACTCCAACCT CCAACGAGGT CACCAAGGCT
    AATGGGCAGT TGGTGCAGCA ACAGCTGGAA TCCCGTGAGA GCGAGGAGGA GCCACAGCAT
    GTCGACGAAG ATCTTTCGGA TGAAGCGTTT ATTATGCGAC ATCAGCGAGC TCTTCTCGAG
    GAGCGACGCC GCTTCGAGAC TTTCCTTAAG TTCCCCTGGA GCACCCGATC GCGTGCAAAT
    AGGCGCGTCG ATAGTCGTGC CGAATCTAGT GGCGCCAACA CACCGGATCC CGCCTCACCA
    GCTCCTCATC TAGGCGGCCC TGGTCACGAC AACGAAAGCA TTCCCTCGCC GCTGGCGTAC
    CCACTGGACG CTTTTAACGA AAGCGGTGAG CTGCTGACTG GCGGACAGGC ACGCCAAGCT
    CGTCGTCGGA CTACGTCTAG CAAGCTTAAG GATCAGCTGG AACGGCGCAG CACCACGCCC
    GATCTGCGGG AGTCTTACAC TGTGATGCCA TCTCCCTTTG AGCCCTTGAA CTTTCCGCTC
    TCCGACGAGG TCTACCAGCG ATTGCTAGCC GAGACATATG CGCCGCCCAG GTCAATGACC
    GTCTCAAAGC GGGCCAAAAG CAAGTCGATA TCCTCGAACT GTGATGGTGG GAACACCTTC
    ACAGGAGGCA GCGGCAGTCG GCGCAGCAGC AAGTGCAAGA TTAAGCCAAA TGGTCAGCTA
    AATGGCCAGC TCAATGGCCA CCCTGCGGCG GCGAAGTTGA ACGGAATAGA TGCGGCAAAT
    GAAGGTGGAA TTTCTGAGCC GGAAGAGGAA GAGATGCTGC TAGAAGACGA GGATGACGAC
    TATCCCAAGC ATCATCTGGC GCCGCTGGAT GACGAGGAGC CGTCGACACC AGATGTCGAG
    CATGAGCTTT ACGACTCGGC GATCGATGCC TATCTCGCTG ATCCGGAGGC GCTAGAGGAG
    GATATGGGCG AGGATCCCTT CGAGGACGAC GATCCCAACG ATCCGGAGTG GAAGACACGG
    ACCGAGGGTG TTCGCAGCAG ACGCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.