Tk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tk
Entrez Gene ID	41456
Full Name	Tachykinin
Synonyms	CG14734,DTK,DTK-1,DTK-2,DTK-3,DTK-4,DTK-5,DTK-6,DTK1,DmTK6,Dmel\CG14734,Drm-TK,Drm-TK-1,Drm-TK-2,Drm-TK-3,Drm-TK-4,Drm-TK-5,Drm-TK-6,Dtk,TAP-5,TK,TK-1,Tyk,dTK,dtk
General protein information	Preferred Names Tachykinin Names CG14734 gene product from transcript CG14734-RB CG14734-PA CG14734-PB Tachykinin-related Tk-PA Tk-PB neurokinin preprotachykinin tachykinin tachykinin-1 tachykinin-3 tachykinin-6 tachykinin-like peptide
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87A4-87A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275564.2	NP_001262493.1	tachykinin, isoform B
NM_141884.4	NP_650141.2	tachykinin, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results highlight a conserved role for Tachykinin signaling in regulating nociception.
Title: Tachykinin acts upstream of autocrine Hedgehog signaling during nociceptive sensitization in Drosophila.

Study identified a small group of sexually dimorphic, FruM+ neurons that promote aggressiveness in Drosophila males but have no influence on male-female courtship behavior. These neurons enhance aggression, at least in part, through the release of a neuropeptide, Drosophila tachykinin.
Title: Tachykinin-expressing neurons control male-specific aggressive arousal in Drosophila.

The control of DILP5 signaling in the renal tubules by Drosophila tachykinin peptide (DTK) and its receptor DTKR during desiccative, nutritional and oxidative stress, was investigated.
Title: Insulin production and signaling in renal tubules of Drosophila is under control of tachykinin-related peptide and regulates stress resistance.

The distribution of the Dtk transcript and peptides was analyzed by in situ hybridization and immunocytochemistry during postembryonic development of the central nervous system (CNS) of Drosophila.
Title: Neuronal expression of tachykinin-related peptides and gene transcript during postembryonic development of Drosophila.

The following Tk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04623	NM_141884.4 Latest version!	Drosophila melanogaster tachykinin, transcript variant A (Tk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08331	NM_001275564.2 Latest version!	Drosophila melanogaster tachykinin, transcript variant B (Tk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tk ( NM_141884.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650141.2 Drosophila melanogaster tachykinin, transcript variant A (Tk), mRNA.

Price & Availability↑

CloneID	OFa04623
Clone ID Related Accession (Same CDS sequence)	NM_141884.4
Accession Version	NM_141884.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tachykinin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141884.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCCCTC TGAGCGGTTT GATAGCGCTG GCGCTGCTGC TGCTGCTCCT GCTGACGGCA 
CCATCGAGTG CGGCGGACAC GGAGACGGAG TCCTCCGGCA GCCCGCTGAC GCCCGGCGCC 
GAAGAGCCGC GTCGGGTGGT AAAGCGGGCG CCCACGTCCA GCTTCATTGG GATGCGCGGC 
AAGAAGGACG AGGAGCACGA CACCTCGGAG GGTAACTGGC TGGGATCGGG ACCCGATCCG 
CTGGATTACG CCGACGAGGA GGCGGACAGC AGCTACGCGG AGAACGGGCG GCGACTGAAG 
AAGGCACCGC TGGCCTTTGT TGGCCTGCGT GGCAAGAAAT TCATCCCAAT CAACAACCGC 
CTGTCCGATG TCCTGCAGAG CCTGGAGGAG GAGCGCCTGC GGGATAGCCT GCTGCAGGAC 
TTCTTCGATC GCGTGGCTGG GCGTGATGGC TCCGCGGTGG GCAAGAGAGC GCCCACCGGG 
TTCACGGGCA TGCGAGGTAA GCGACCCGCC CTGCTGGCCG GGGACGATGA CGCGGAGGCG 
GACGAGGCCA CGGAGCTGCA ACAAAAGCGG GCGCCGGTCA ATTCCTTTGT GGGGATGCGC 
GGCAAGAAGG ACGTCTCCCA CCAGCACTAC AAGCGTGCAG CTCTCTCCGA TTCCTATGAC 
TTGAGAGGAA AACAGCAGCG CTTCGCCGAC TTCAACAGCA AATTTGTGGC GGTGCGTGGC 
AAGAAGAGCG ATCTGGAGGG CAACGGAGTC GGGATTGGTG ACGATCATGA GCAAGCTCTG 
GTGCATCCAT GGCTCTACCT GTGGGGAGAA AAGCGAGCGC CCAACGGATT CCTGGGAATG 
CGAGGCAAAC GGCCAGCACT TTTCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650141.2
CDS	121..990
Translation	MRPLSGLIALALLLLLLLTAPSSAADTETESSGSPLTPGAEEPRRVVKRAPTSSFIGMRGKKDEEHDTSEGNWLGSGPDPLDYADEEADSSYAENGRRLKKAPLAFVGLRGKKFIPINNRLSDVLQSLEEERLRDSLLQDFFDRVAGRDGSAVGKRAPTGFTGMRGKRPALLAGDDDAEADEATELQQKRAPVNSFVGMRGKKDVSHQHYKRAALSDSYDLRGKQQRFADFNSKFVAVRGKKSDLEGNGVGIGDDHEQALVHPWLYLWGEKRAPNGFLGMRGKRPALFE

Target ORF information:

RefSeq Version	NM_141884.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tachykinin, transcript variant A (Tk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141884.4

ORF Insert Sequence:

ATGCGCCCTC TGAGCGGTTT GATAGCGCTG GCGCTGCTGC TGCTGCTCCT GCTGACGGCA 
CCATCGAGTG CGGCGGACAC GGAGACGGAG TCCTCCGGCA GCCCGCTGAC GCCCGGCGCC 
GAAGAGCCGC GTCGGGTGGT AAAGCGGGCG CCCACGTCCA GCTTCATTGG GATGCGCGGC 
AAGAAGGACG AGGAGCACGA CACCTCGGAG GGTAACTGGC TGGGATCGGG ACCCGATCCG 
CTGGATTACG CCGACGAGGA GGCGGACAGC AGCTACGCGG AGAACGGGCG GCGACTGAAG 
AAGGCACCGC TGGCCTTTGT TGGCCTGCGT GGCAAGAAAT TCATCCCAAT CAACAACCGC 
CTGTCCGATG TCCTGCAGAG CCTGGAGGAG GAGCGCCTGC GGGATAGCCT GCTGCAGGAC 
TTCTTCGATC GCGTGGCTGG GCGTGATGGC TCCGCGGTGG GCAAGAGAGC GCCCACCGGG 
TTCACGGGCA TGCGAGGTAA GCGACCCGCC CTGCTGGCCG GGGACGATGA CGCGGAGGCG 
GACGAGGCCA CGGAGCTGCA ACAAAAGCGG GCGCCGGTCA ATTCCTTTGT GGGGATGCGC 
GGCAAGAAGG ACGTCTCCCA CCAGCACTAC AAGCGTGCAG CTCTCTCCGA TTCCTATGAC 
TTGAGAGGAA AACAGCAGCG CTTCGCCGAC TTCAACAGCA AATTTGTGGC GGTGCGTGGC 
AAGAAGAGCG ATCTGGAGGG CAACGGAGTC GGGATTGGTG ACGATCATGA GCAAGCTCTG 
GTGCATCCAT GGCTCTACCT GTGGGGAGAA AAGCGAGCGC CCAACGGATT CCTGGGAATG 
CGAGGCAAAC GGCCAGCACT TTTCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tk ( NM_001275564.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262493.1 Drosophila melanogaster tachykinin, transcript variant B (Tk), mRNA.

Price & Availability↑

CloneID	OFa08331
Clone ID Related Accession (Same CDS sequence)	NM_001275564.2
Accession Version	NM_001275564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tachykinin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275564.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCCCTC TGAGCGGTTT GATAGCGCTG GCGCTGCTGC TGCTGCTCCT GCTGACGGCA 
CCATCGAGTG CGGCGGACAC GGAGACGGAG TCCTCCGGCA GCCCGCTGAC GCCCGGCGCC 
GAAGAGCCGC GTCGGGTGGT AAAGCGGGCG CCCACGTCCA GCTTCATTGG GATGCGCGGC 
AAGAAGGACG AGGAGCACGA CACCTCGGAG GGTAACTGGC TGGGATCGGG ACCCGATCCG 
CTGGATTACG CCGACGAGGA GGCGGACAGC AGCTACGCGG AGAACGGGCG GCGACTGAAG 
AAGGCACCGC TGGCCTTTGT TGGCCTGCGT GGCAAGAAAT TCATCCCAAT CAACAACCGC 
CTGTCCGATG TCCTGCAGAG CCTGGAGGAG GAGCGCCTGC GGGATAGCCT GCTGCAGGAC 
TTCTTCGATC GCGTGGCTGG GCGTGATGGC TCCGCGGTGG GCAAGAGAGC GCCCACCGGG 
TTCACGGGCA TGCGAGGTAA GCGACCCGCC CTGCTGGCCG GGGACGATGA CGCGGAGGCG 
GACGAGGCCA CGGAGCTGCA ACAAAAGCGG GCGCCGGTCA ATTCCTTTGT GGGGATGCGC 
GGCAAGAAGG ACGTCTCCCA CCAGCACTAC AAGCGTGCAG CTCTCTCCGA TTTCTGGCAC 
ACTTTTTTTA AAAAGTCCTA TGACTTGAGA GGAAAACAGC AGCGCTTCGC CGACTTCAAC 
AGCAAATTTG TGGCGGTGCG TGGCAAGAAG AGCGATCTGG AGGGCAACGG AGTCGGGATT 
GGTGACGATC ATGAGCAAGC TCTGGTGCAT CCATGGCTCT ACCTGTGGGG AGAAAAGCGA 
GCGCCCAACG GATTCCTGGG AATGCGAGGC AAACGGCCAG CACTTTTCGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262493.1
CDS	158..1051
Translation	MRPLSGLIALALLLLLLLTAPSSAADTETESSGSPLTPGAEEPRRVVKRAPTSSFIGMRGKKDEEHDTSEGNWLGSGPDPLDYADEEADSSYAENGRRLKKAPLAFVGLRGKKFIPINNRLSDVLQSLEEERLRDSLLQDFFDRVAGRDGSAVGKRAPTGFTGMRGKRPALLAGDDDAEADEATELQQKRAPVNSFVGMRGKKDVSHQHYKRAALSDFWHTFFKKSYDLRGKQQRFADFNSKFVAVRGKKSDLEGNGVGIGDDHEQALVHPWLYLWGEKRAPNGFLGMRGKRPALFE

Target ORF information:

RefSeq Version	NM_001275564.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tachykinin, transcript variant B (Tk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275564.2

ORF Insert Sequence:

ATGCGCCCTC TGAGCGGTTT GATAGCGCTG GCGCTGCTGC TGCTGCTCCT GCTGACGGCA 
CCATCGAGTG CGGCGGACAC GGAGACGGAG TCCTCCGGCA GCCCGCTGAC GCCCGGCGCC 
GAAGAGCCGC GTCGGGTGGT AAAGCGGGCG CCCACGTCCA GCTTCATTGG GATGCGCGGC 
AAGAAGGACG AGGAGCACGA CACCTCGGAG GGTAACTGGC TGGGATCGGG ACCCGATCCG 
CTGGATTACG CCGACGAGGA GGCGGACAGC AGCTACGCGG AGAACGGGCG GCGACTGAAG 
AAGGCACCGC TGGCCTTTGT TGGCCTGCGT GGCAAGAAAT TCATCCCAAT CAACAACCGC 
CTGTCCGATG TCCTGCAGAG CCTGGAGGAG GAGCGCCTGC GGGATAGCCT GCTGCAGGAC 
TTCTTCGATC GCGTGGCTGG GCGTGATGGC TCCGCGGTGG GCAAGAGAGC GCCCACCGGG 
TTCACGGGCA TGCGAGGTAA GCGACCCGCC CTGCTGGCCG GGGACGATGA CGCGGAGGCG 
GACGAGGCCA CGGAGCTGCA ACAAAAGCGG GCGCCGGTCA ATTCCTTTGT GGGGATGCGC 
GGCAAGAAGG ACGTCTCCCA CCAGCACTAC AAGCGTGCAG CTCTCTCCGA TTTCTGGCAC 
ACTTTTTTTA AAAAGTCCTA TGACTTGAGA GGAAAACAGC AGCGCTTCGC CGACTTCAAC 
AGCAAATTTG TGGCGGTGCG TGGCAAGAAG AGCGATCTGG AGGGCAACGG AGTCGGGATT 
GGTGACGATC ATGAGCAAGC TCTGGTGCAT CCATGGCTCT ACCTGTGGGG AGAAAAGCGA 
GCGCCCAACG GATTCCTGGG AATGCGAGGC AAACGGCCAG CACTTTTCGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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