mfas cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following mfas gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mfas cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa04617 NM_176466.1
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa04617 NM_176467.1
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant N, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa04618 NM_169444.1
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa04618 NM_176470.1
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant P, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa04618 NM_176468.1
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa04618 NM_176469.2
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa04619 NM_176472.1
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa04619 NM_176471.1
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa04619 NM_079600.3
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa04620 NM_176474.1
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant O, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa04620 NM_176473.1
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa04621 NM_176477.1
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant M, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa04621 NM_176475.1
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa04621 NM_176476.1
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa04622 NM_176478.1
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa08330 NM_001260137.1
Latest version!
Drosophila melanogaster midline fasciclin (mfas), transcript variant Q, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa04617
    Clone ID Related Accession (Same CDS sequence) NM_176467.1 , NM_176466.1
    Accession Version NM_176467.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2448bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform N
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACGGATACGA CACCGAGTTC GTGCCACTGG AGCACCAGCA GCAGCCGCAG
    GGACGCCAGG AGACGGCGGC CACCGTTCCC GCTCAAACCC CCAGCGGTGT GGAGCAGAAG
    CCGTTCAATG TGGACACCAT CACCACGGAT GTGAATTCCC CAAATCCGGC CATCTTCTTC
    CAGCAATCGT TCCCCTTCTT TGGCAATGAG TTCTTCAATT CGTTCGGAGG CTTTGGCTTT
    GGAGCTGCCC AGGAGCCCTG GTGGAAGGGA CCCAATGTGT GCACCGAAAA GGAGGAAGAC
    GAGACTGTGG CCAGTGAAAC GGAGGCAGAA GAAACCGTGG ACACCTTTGG CCAGGAACGT
    GATGGCGTTA CCAACGTGGT GCGATCCCCA CTCTTTGGCC AGTTCCAGTT CAGCGTTAAC
    TCCTGCGCCG AGAAGCCCAA CAAGCACGTC TGCACCAAGA TCGTGAATCA AAATGGCAAG
    AAGAAAACGC TGACGCTGAC CCGCCAGTGC TGCTATGGAT ATGGACGCCC CAGAAACGCT
    GACTTCACAA CGCCCTGCGA GAAGATCGAC ATCAAGGACG TGGAGGCCAC GGCCAGTGAC
    ATGGGTGCCA AGCAGTTCCT GGAGAGTGCA CGCACCGCCG GTGAGCTGGC CGATATGCTC
    GGAGCAGGCA GTGGCAAGAA GGTGACCCTC TTCGTGCCCA ACGACGCCGC CTTCATGGAG
    TACCGTGGTC ATCATCAGCA GGAGAATAAT GTGGAAGATG CCAAGGCCGA GGCTTCCAAG
    CCATCCATCT ACAAACTGCA CGCTGTTCTC GGCGAAGTTC AGCTGGAGGA TGTGCCCAAT
    GAGAAGCTTC TGCAGACGGA GCTGCCTGAC CAGAAGATCC GCATCAACAG CTACCAGCTG
    CCTGCGGCTT TGGGCCTGGA ACCCTACCGC TTTGCCGCCA ACTGTGTGCC CATCGAGAAG
    CACGACAAGC TCTCGGAGCA GGCCCTTGTC CACACTCTGG GCGGAGTCCT GAAGCCGCTG
    ACCAAGAACC TTATGGATAT CATCAGGGAG CGTGCGGATA TGTCCATTAT GCGCACCGTT
    CTGGAGAAGA CCAACCTGAG CGCCATGCTA GAGGATGACA AGCCAGTGAC CATCTTTGTG
    CCCACCGATG CCGCCTTCGA CAAGTTGGAA CCTCATCTGC GTCGAGCCCT CAAGGAAGGT
    CGCGGTTGTG CCTCCAACAT CCTGAAGAAC CATTTGCTGG ACCTCACCTT CTGCTCGCTG
    GCCACTGTTC CGGGTGCCAA GACGACGGCC TACAATCTAC TGGGAGAACC CCTGCTCCTC
    AACCGAACCC ACCGCGCTGC GAATCAGACT GGACCGACTC CCATCTACAT CAATAACTTG
    GCCAAAATCA TTGATGCCGA TATCATGGGC ACCAATGGTG TGCTGCACGT AATCGACACC
    ATCCTGCCCA CCGAATCGGC TCTGCCCATG ACCTCGCTGA TGTCGCAAAA GAACCTGACC
    ATCTTCCAGC GATTGCTGGA GGCCTCCGGC TACGATGATC AGTTCGACGA TCTGGACAAC
    GTGACCATCT TTGCACCAAC TGACAAGGCT CTGCAGAATA CGGAATGGGC TCGCATGCTC
    AAGGAACAGC CGGAGCTGCT GAATCATAAC TTGGATCTGC TGGAGTTCCT TAACTACCAT
    GTGGTCAAGC CCATGATCAA GACCTGTGAC CTGTCGGAGA AGTCCCTGCC CACGGTTGCT
    GGATCTAGTG TGCGTCTGAA CCTGTACTCC ACCCATGCTC TCTTCTCGGA CGTGATGAAC
    CGGGCAACGG TAAACTGTGC CCGTTTGGTG CACTTCGATG ACGAGAGCTG CGGATCCGTT
    CTCCACCAGG TGGATCGTGC CTTGGCGCCT CCCAAGAATA ACATGCTTAA GCTGCTGGAG
    GCTAATCCCA ACTACAGCAA GTTCCTGGAG CTGGTTCGTA AGGCTAATCT TACACAGCTG
    CTGTCCAACG ATTCGAGGAG TCTCACCCTG CTGGTGCCAA AGAACGATAT CTTCGAGGAG
    CTGAACGAGT CCGGTGAGGG TTCAAAGCCC GCCGATCCCA TGGCCTTGGT CAAAACCCAT
    ATCGTTGAGG ATGTTGTCTG CTGTGCCGGC ATTATCCCAA CCAACTGGCC ATTTGTCCGC
    TCCATCGAGT CCATCTCTGG CCAGCATCTG CGTATCACCC GCGATCGTCG TCCCAAGATC
    GAGAACGCCG GCGTCACCAA GTGCGATGTG GTGGCCACCA ATGGAATTCT CCATGAGATC
    AACGACATCA TCGTGCCACG CCCACAGCGC CAGCAACAGC AGCGACCCCA GCTGATCCCG
    CCCGGAGCTG GCTATCAGCC ACAGGGCGAT TTCGATGTCT TCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788644.1
    CDS108..2555
    Translation

    Target ORF information:

    RefSeq Version NM_176467.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant N, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176467.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACGGATACGA CACCGAGTTC GTGCCACTGG AGCACCAGCA GCAGCCGCAG
    GGACGCCAGG AGACGGCGGC CACCGTTCCC GCTCAAACCC CCAGCGGTGT GGAGCAGAAG
    CCGTTCAATG TGGACACCAT CACCACGGAT GTGAATTCCC CAAATCCGGC CATCTTCTTC
    CAGCAATCGT TCCCCTTCTT TGGCAATGAG TTCTTCAATT CGTTCGGAGG CTTTGGCTTT
    GGAGCTGCCC AGGAGCCCTG GTGGAAGGGA CCCAATGTGT GCACCGAAAA GGAGGAAGAC
    GAGACTGTGG CCAGTGAAAC GGAGGCAGAA GAAACCGTGG ACACCTTTGG CCAGGAACGT
    GATGGCGTTA CCAACGTGGT GCGATCCCCA CTCTTTGGCC AGTTCCAGTT CAGCGTTAAC
    TCCTGCGCCG AGAAGCCCAA CAAGCACGTC TGCACCAAGA TCGTGAATCA AAATGGCAAG
    AAGAAAACGC TGACGCTGAC CCGCCAGTGC TGCTATGGAT ATGGACGCCC CAGAAACGCT
    GACTTCACAA CGCCCTGCGA GAAGATCGAC ATCAAGGACG TGGAGGCCAC GGCCAGTGAC
    ATGGGTGCCA AGCAGTTCCT GGAGAGTGCA CGCACCGCCG GTGAGCTGGC CGATATGCTC
    GGAGCAGGCA GTGGCAAGAA GGTGACCCTC TTCGTGCCCA ACGACGCCGC CTTCATGGAG
    TACCGTGGTC ATCATCAGCA GGAGAATAAT GTGGAAGATG CCAAGGCCGA GGCTTCCAAG
    CCATCCATCT ACAAACTGCA CGCTGTTCTC GGCGAAGTTC AGCTGGAGGA TGTGCCCAAT
    GAGAAGCTTC TGCAGACGGA GCTGCCTGAC CAGAAGATCC GCATCAACAG CTACCAGCTG
    CCTGCGGCTT TGGGCCTGGA ACCCTACCGC TTTGCCGCCA ACTGTGTGCC CATCGAGAAG
    CACGACAAGC TCTCGGAGCA GGCCCTTGTC CACACTCTGG GCGGAGTCCT GAAGCCGCTG
    ACCAAGAACC TTATGGATAT CATCAGGGAG CGTGCGGATA TGTCCATTAT GCGCACCGTT
    CTGGAGAAGA CCAACCTGAG CGCCATGCTA GAGGATGACA AGCCAGTGAC CATCTTTGTG
    CCCACCGATG CCGCCTTCGA CAAGTTGGAA CCTCATCTGC GTCGAGCCCT CAAGGAAGGT
    CGCGGTTGTG CCTCCAACAT CCTGAAGAAC CATTTGCTGG ACCTCACCTT CTGCTCGCTG
    GCCACTGTTC CGGGTGCCAA GACGACGGCC TACAATCTAC TGGGAGAACC CCTGCTCCTC
    AACCGAACCC ACCGCGCTGC GAATCAGACT GGACCGACTC CCATCTACAT CAATAACTTG
    GCCAAAATCA TTGATGCCGA TATCATGGGC ACCAATGGTG TGCTGCACGT AATCGACACC
    ATCCTGCCCA CCGAATCGGC TCTGCCCATG ACCTCGCTGA TGTCGCAAAA GAACCTGACC
    ATCTTCCAGC GATTGCTGGA GGCCTCCGGC TACGATGATC AGTTCGACGA TCTGGACAAC
    GTGACCATCT TTGCACCAAC TGACAAGGCT CTGCAGAATA CGGAATGGGC TCGCATGCTC
    AAGGAACAGC CGGAGCTGCT GAATCATAAC TTGGATCTGC TGGAGTTCCT TAACTACCAT
    GTGGTCAAGC CCATGATCAA GACCTGTGAC CTGTCGGAGA AGTCCCTGCC CACGGTTGCT
    GGATCTAGTG TGCGTCTGAA CCTGTACTCC ACCCATGCTC TCTTCTCGGA CGTGATGAAC
    CGGGCAACGG TAAACTGTGC CCGTTTGGTG CACTTCGATG ACGAGAGCTG CGGATCCGTT
    CTCCACCAGG TGGATCGTGC CTTGGCGCCT CCCAAGAATA ACATGCTTAA GCTGCTGGAG
    GCTAATCCCA ACTACAGCAA GTTCCTGGAG CTGGTTCGTA AGGCTAATCT TACACAGCTG
    CTGTCCAACG ATTCGAGGAG TCTCACCCTG CTGGTGCCAA AGAACGATAT CTTCGAGGAG
    CTGAACGAGT CCGGTGAGGG TTCAAAGCCC GCCGATCCCA TGGCCTTGGT CAAAACCCAT
    ATCGTTGAGG ATGTTGTCTG CTGTGCCGGC ATTATCCCAA CCAACTGGCC ATTTGTCCGC
    TCCATCGAGT CCATCTCTGG CCAGCATCTG CGTATCACCC GCGATCGTCG TCCCAAGATC
    GAGAACGCCG GCGTCACCAA GTGCGATGTG GTGGCCACCA ATGGAATTCT CCATGAGATC
    AACGACATCA TCGTGCCACG CCCACAGCGC CAGCAACAGC AGCGACCCCA GCTGATCCCG
    CCCGGAGCTG GCTATCAGCC ACAGGGCGAT TTCGATGTCT TCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04617
    Clone ID Related Accession (Same CDS sequence) NM_176467.1 , NM_176466.1
    Accession Version NM_176466.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2448bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACGGATACGA CACCGAGTTC GTGCCACTGG AGCACCAGCA GCAGCCGCAG
    GGACGCCAGG AGACGGCGGC CACCGTTCCC GCTCAAACCC CCAGCGGTGT GGAGCAGAAG
    CCGTTCAATG TGGACACCAT CACCACGGAT GTGAATTCCC CAAATCCGGC CATCTTCTTC
    CAGCAATCGT TCCCCTTCTT TGGCAATGAG TTCTTCAATT CGTTCGGAGG CTTTGGCTTT
    GGAGCTGCCC AGGAGCCCTG GTGGAAGGGA CCCAATGTGT GCACCGAAAA GGAGGAAGAC
    GAGACTGTGG CCAGTGAAAC GGAGGCAGAA GAAACCGTGG ACACCTTTGG CCAGGAACGT
    GATGGCGTTA CCAACGTGGT GCGATCCCCA CTCTTTGGCC AGTTCCAGTT CAGCGTTAAC
    TCCTGCGCCG AGAAGCCCAA CAAGCACGTC TGCACCAAGA TCGTGAATCA AAATGGCAAG
    AAGAAAACGC TGACGCTGAC CCGCCAGTGC TGCTATGGAT ATGGACGCCC CAGAAACGCT
    GACTTCACAA CGCCCTGCGA GAAGATCGAC ATCAAGGACG TGGAGGCCAC GGCCAGTGAC
    ATGGGTGCCA AGCAGTTCCT GGAGAGTGCA CGCACCGCCG GTGAGCTGGC CGATATGCTC
    GGAGCAGGCA GTGGCAAGAA GGTGACCCTC TTCGTGCCCA ACGACGCCGC CTTCATGGAG
    TACCGTGGTC ATCATCAGCA GGAGAATAAT GTGGAAGATG CCAAGGCCGA GGCTTCCAAG
    CCATCCATCT ACAAACTGCA CGCTGTTCTC GGCGAAGTTC AGCTGGAGGA TGTGCCCAAT
    GAGAAGCTTC TGCAGACGGA GCTGCCTGAC CAGAAGATCC GCATCAACAG CTACCAGCTG
    CCTGCGGCTT TGGGCCTGGA ACCCTACCGC TTTGCCGCCA ACTGTGTGCC CATCGAGAAG
    CACGACAAGC TCTCGGAGCA GGCCCTTGTC CACACTCTGG GCGGAGTCCT GAAGCCGCTG
    ACCAAGAACC TTATGGATAT CATCAGGGAG CGTGCGGATA TGTCCATTAT GCGCACCGTT
    CTGGAGAAGA CCAACCTGAG CGCCATGCTA GAGGATGACA AGCCAGTGAC CATCTTTGTG
    CCCACCGATG CCGCCTTCGA CAAGTTGGAA CCTCATCTGC GTCGAGCCCT CAAGGAAGGT
    CGCGGTTGTG CCTCCAACAT CCTGAAGAAC CATTTGCTGG ACCTCACCTT CTGCTCGCTG
    GCCACTGTTC CGGGTGCCAA GACGACGGCC TACAATCTAC TGGGAGAACC CCTGCTCCTC
    AACCGAACCC ACCGCGCTGC GAATCAGACT GGACCGACTC CCATCTACAT CAATAACTTG
    GCCAAAATCA TTGATGCCGA TATCATGGGC ACCAATGGTG TGCTGCACGT AATCGACACC
    ATCCTGCCCA CCGAATCGGC TCTGCCCATG ACCTCGCTGA TGTCGCAAAA GAACCTGACC
    ATCTTCCAGC GATTGCTGGA GGCCTCCGGC TACGATGATC AGTTCGACGA TCTGGACAAC
    GTGACCATCT TTGCACCAAC TGACAAGGCT CTGCAGAATA CGGAATGGGC TCGCATGCTC
    AAGGAACAGC CGGAGCTGCT GAATCATAAC TTGGATCTGC TGGAGTTCCT TAACTACCAT
    GTGGTCAAGC CCATGATCAA GACCTGTGAC CTGTCGGAGA AGTCCCTGCC CACGGTTGCT
    GGATCTAGTG TGCGTCTGAA CCTGTACTCC ACCCATGCTC TCTTCTCGGA CGTGATGAAC
    CGGGCAACGG TAAACTGTGC CCGTTTGGTG CACTTCGATG ACGAGAGCTG CGGATCCGTT
    CTCCACCAGG TGGATCGTGC CTTGGCGCCT CCCAAGAATA ACATGCTTAA GCTGCTGGAG
    GCTAATCCCA ACTACAGCAA GTTCCTGGAG CTGGTTCGTA AGGCTAATCT TACACAGCTG
    CTGTCCAACG ATTCGAGGAG TCTCACCCTG CTGGTGCCAA AGAACGATAT CTTCGAGGAG
    CTGAACGAGT CCGGTGAGGG TTCAAAGCCC GCCGATCCCA TGGCCTTGGT CAAAACCCAT
    ATCGTTGAGG ATGTTGTCTG CTGTGCCGGC ATTATCCCAA CCAACTGGCC ATTTGTCCGC
    TCCATCGAGT CCATCTCTGG CCAGCATCTG CGTATCACCC GCGATCGTCG TCCCAAGATC
    GAGAACGCCG GCGTCACCAA GTGCGATGTG GTGGCCACCA ATGGAATTCT CCATGAGATC
    AACGACATCA TCGTGCCACG CCCACAGCGC CAGCAACAGC AGCGACCCCA GCTGATCCCG
    CCCGGAGCTG GCTATCAGCC ACAGGGCGAT TTCGATGTCT TCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788643.1
    CDS130..2577
    Translation

    Target ORF information:

    RefSeq Version NM_176466.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176466.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACGGATACGA CACCGAGTTC GTGCCACTGG AGCACCAGCA GCAGCCGCAG
    GGACGCCAGG AGACGGCGGC CACCGTTCCC GCTCAAACCC CCAGCGGTGT GGAGCAGAAG
    CCGTTCAATG TGGACACCAT CACCACGGAT GTGAATTCCC CAAATCCGGC CATCTTCTTC
    CAGCAATCGT TCCCCTTCTT TGGCAATGAG TTCTTCAATT CGTTCGGAGG CTTTGGCTTT
    GGAGCTGCCC AGGAGCCCTG GTGGAAGGGA CCCAATGTGT GCACCGAAAA GGAGGAAGAC
    GAGACTGTGG CCAGTGAAAC GGAGGCAGAA GAAACCGTGG ACACCTTTGG CCAGGAACGT
    GATGGCGTTA CCAACGTGGT GCGATCCCCA CTCTTTGGCC AGTTCCAGTT CAGCGTTAAC
    TCCTGCGCCG AGAAGCCCAA CAAGCACGTC TGCACCAAGA TCGTGAATCA AAATGGCAAG
    AAGAAAACGC TGACGCTGAC CCGCCAGTGC TGCTATGGAT ATGGACGCCC CAGAAACGCT
    GACTTCACAA CGCCCTGCGA GAAGATCGAC ATCAAGGACG TGGAGGCCAC GGCCAGTGAC
    ATGGGTGCCA AGCAGTTCCT GGAGAGTGCA CGCACCGCCG GTGAGCTGGC CGATATGCTC
    GGAGCAGGCA GTGGCAAGAA GGTGACCCTC TTCGTGCCCA ACGACGCCGC CTTCATGGAG
    TACCGTGGTC ATCATCAGCA GGAGAATAAT GTGGAAGATG CCAAGGCCGA GGCTTCCAAG
    CCATCCATCT ACAAACTGCA CGCTGTTCTC GGCGAAGTTC AGCTGGAGGA TGTGCCCAAT
    GAGAAGCTTC TGCAGACGGA GCTGCCTGAC CAGAAGATCC GCATCAACAG CTACCAGCTG
    CCTGCGGCTT TGGGCCTGGA ACCCTACCGC TTTGCCGCCA ACTGTGTGCC CATCGAGAAG
    CACGACAAGC TCTCGGAGCA GGCCCTTGTC CACACTCTGG GCGGAGTCCT GAAGCCGCTG
    ACCAAGAACC TTATGGATAT CATCAGGGAG CGTGCGGATA TGTCCATTAT GCGCACCGTT
    CTGGAGAAGA CCAACCTGAG CGCCATGCTA GAGGATGACA AGCCAGTGAC CATCTTTGTG
    CCCACCGATG CCGCCTTCGA CAAGTTGGAA CCTCATCTGC GTCGAGCCCT CAAGGAAGGT
    CGCGGTTGTG CCTCCAACAT CCTGAAGAAC CATTTGCTGG ACCTCACCTT CTGCTCGCTG
    GCCACTGTTC CGGGTGCCAA GACGACGGCC TACAATCTAC TGGGAGAACC CCTGCTCCTC
    AACCGAACCC ACCGCGCTGC GAATCAGACT GGACCGACTC CCATCTACAT CAATAACTTG
    GCCAAAATCA TTGATGCCGA TATCATGGGC ACCAATGGTG TGCTGCACGT AATCGACACC
    ATCCTGCCCA CCGAATCGGC TCTGCCCATG ACCTCGCTGA TGTCGCAAAA GAACCTGACC
    ATCTTCCAGC GATTGCTGGA GGCCTCCGGC TACGATGATC AGTTCGACGA TCTGGACAAC
    GTGACCATCT TTGCACCAAC TGACAAGGCT CTGCAGAATA CGGAATGGGC TCGCATGCTC
    AAGGAACAGC CGGAGCTGCT GAATCATAAC TTGGATCTGC TGGAGTTCCT TAACTACCAT
    GTGGTCAAGC CCATGATCAA GACCTGTGAC CTGTCGGAGA AGTCCCTGCC CACGGTTGCT
    GGATCTAGTG TGCGTCTGAA CCTGTACTCC ACCCATGCTC TCTTCTCGGA CGTGATGAAC
    CGGGCAACGG TAAACTGTGC CCGTTTGGTG CACTTCGATG ACGAGAGCTG CGGATCCGTT
    CTCCACCAGG TGGATCGTGC CTTGGCGCCT CCCAAGAATA ACATGCTTAA GCTGCTGGAG
    GCTAATCCCA ACTACAGCAA GTTCCTGGAG CTGGTTCGTA AGGCTAATCT TACACAGCTG
    CTGTCCAACG ATTCGAGGAG TCTCACCCTG CTGGTGCCAA AGAACGATAT CTTCGAGGAG
    CTGAACGAGT CCGGTGAGGG TTCAAAGCCC GCCGATCCCA TGGCCTTGGT CAAAACCCAT
    ATCGTTGAGG ATGTTGTCTG CTGTGCCGGC ATTATCCCAA CCAACTGGCC ATTTGTCCGC
    TCCATCGAGT CCATCTCTGG CCAGCATCTG CGTATCACCC GCGATCGTCG TCCCAAGATC
    GAGAACGCCG GCGTCACCAA GTGCGATGTG GTGGCCACCA ATGGAATTCT CCATGAGATC
    AACGACATCA TCGTGCCACG CCCACAGCGC CAGCAACAGC AGCGACCCCA GCTGATCCCG
    CCCGGAGCTG GCTATCAGCC ACAGGGCGAT TTCGATGTCT TCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04618
    Clone ID Related Accession (Same CDS sequence) NM_169444.1 , NM_176470.1 , NM_176469.2 , NM_176468.1
    Accession Version NM_169444.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACAGCGGATA CGACACCGAG TTCGTGCCAC TGGAGCACCA GCAGCAGCCG
    CAGGGACGCC AGGAGACGGC GGCCACCGTT CCCGCTCAAA CCCCCAGCGG TGTGGAGCAG
    AAGCCGTTCA ATGTGGACAC CATCACCACG GATGTGAATT CCCCAAATCC GGCCATCTTC
    TTCCAGCAAT CGTTCCCCTT CTTTGGCAAT GAGTTCTTCA ATTCGTTCGG AGGCTTTGGC
    TTTGGAGCTG CCCAGGAGCC CTGGTGGAAG GGACCCAATG TGTGCACCGA AAAGGAGGAA
    GACGAGACTG TGGCCAGTGA AACGGAGGCA GAAGAAACCG TGGACACCTT TGGCCAGGAA
    CGTGATGGCG TTACCAACGT GGTGCGATCC CCACTCTTTG GCCAGTTCCA GTTCAGCGTT
    AACTCCTGCG CCGAGAAGCC CAACAAGCAC GTCTGCACCA AGATCGTGAA TCAAAATGGC
    AAGAAGAAAA CGCTGACGCT GACCCGCCAG TGCTGCTATG GATATGGACG CCCCAGAAAC
    GCTGACTTCA CAACGCCCTG CGAGAAGATC GACATCAAGG ACGTGGAGGC CACGGCCAGT
    GACATGGGTG CCAAGCAGTT CCTGGAGAGT GCACGCACCG CCGGTGAGCT GGCCGATATG
    CTCGGAGCAG GCAGTGGCAA GAAGGTGACC CTCTTCGTGC CCAACGACGC CGCCTTCATG
    GAGTACCGTG GTCATCATCA GCAGGAGAAT AATGTGGAAG ATGCCAAGGC CGAGGCTTCC
    AAGCCATCCA TCTACAAACT GCACGCTGTT CTCGGCGAAG TTCAGCTGGA GGATGTGCCC
    AATGAGAAGC TTCTGCAGAC GGAGCTGCCT GACCAGAAGA TCCGCATCAA CAGCTACCAG
    CTGCCTGCGG CTTTGGGCCT GGAACCCTAC CGCTTTGCCG CCAACTGTGT GCCCATCGAG
    AAGCACGACA AGCTCTCGGA GCAGGCCCTT GTCCACACTC TGGGCGGAGT CCTGAAGCCG
    CTGACCAAGA ACCTTATGGA TATCATCAGG GAGCGTGCGG ATATGTCCAT TATGCGCACC
    GTTCTGGAGA AGACCAACCT GAGCGCCATG CTAGAGGATG ACAAGCCAGT GACCATCTTT
    GTGCCCACCG ATGCCGCCTT CGACAAGTTG GAACCTCATC TGCGTCGAGC CCTCAAGGAA
    GGTCGCGGTT GTGCCTCCAA CATCCTGAAG AACCATTTGC TGGACCTCAC CTTCTGCTCG
    CTGGCCACTG TTCCGGGTGC CAAGACGACG GCCTACAATC TACTGGGAGA ACCCCTGCTC
    CTCAACCGAA CCCACCGCGC TGCGAATCAG ACTGGACCGA CTCCCATCTA CATCAATAAC
    TTGGCCAAAA TCATTGATGC CGATATCATG GGCACCAATG GTGTGCTGCA CGTAATCGAC
    ACCATCCTGC CCACCGAATC GGCTCTGCCC ATGACCTCGC TGATGTCGCA AAAGAACCTG
    ACCATCTTCC AGCGATTGCT GGAGGCCTCC GGCTACGATG ATCAGTTCGA CGATCTGGAC
    AACGTGACCA TCTTTGCACC AACTGACAAG GCTCTGCAGA ATACGGAATG GGCTCGCATG
    CTCAAGGAAC AGCCGGAGCT GCTGAATCAT AACTTGGATC TGCTGGAGTT CCTTAACTAC
    CATGTGGTCA AGCCCATGAT CAAGACCTGT GACCTGTCGG AGAAGTCCCT GCCCACGGTT
    GCTGGATCTA GTGTGCGTCT GAACCTGTAC TCCACCCATG CTCTCTTCTC GGACGTGATG
    AACCGGGCAA CGGTAAACTG TGCCCGTTTG GTGCACTTCG ATGACGAGAG CTGCGGATCC
    GTTCTCCACC AGGTGGATCG TGCCTTGGCG CCTCCCAAGA ATAACATGCT TAAGCTGCTG
    GAGGCTAATC CCAACTACAG CAAGTTCCTG GAGCTGGTTC GTAAGGCTAA TCTTACACAG
    CTGCTGTCCA ACGATTCGAG GAGTCTCACC CTGCTGGTGC CAAAGAACGA TATCTTCGAG
    GAGCTGAACG AGTCCGGTGA GGGTTCAAAG CCCGCCGATC CCATGGCCTT GGTCAAAACC
    CATATCGTTG AGGATGTTGT CTGCTGTGCC GGCATTATCC CAACCAACTG GCCATTTGTC
    CGCTCCATCG AGTCCATCTC TGGCCAGCAT CTGCGTATCA CCCGCGATCG TCGTCCCAAG
    ATCGAGAACG CCGGCGTCAC CAAGTGCGAT GTGGTGGCCA CCAATGGAAT TCTCCATGAG
    ATCAACGACA TCATCGTGCC ACGCCCACAG CGCCAGCAAC AGCAGCGACC CCAGCTGATC
    CCGCCCGGAG CTGGCTATCA GCCACAGGGC GATTTCGATG TCTTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_731661.1
    CDS108..2558
    Translation

    Target ORF information:

    RefSeq Version NM_169444.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169444.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACAGCGGATA CGACACCGAG TTCGTGCCAC TGGAGCACCA GCAGCAGCCG
    CAGGGACGCC AGGAGACGGC GGCCACCGTT CCCGCTCAAA CCCCCAGCGG TGTGGAGCAG
    AAGCCGTTCA ATGTGGACAC CATCACCACG GATGTGAATT CCCCAAATCC GGCCATCTTC
    TTCCAGCAAT CGTTCCCCTT CTTTGGCAAT GAGTTCTTCA ATTCGTTCGG AGGCTTTGGC
    TTTGGAGCTG CCCAGGAGCC CTGGTGGAAG GGACCCAATG TGTGCACCGA AAAGGAGGAA
    GACGAGACTG TGGCCAGTGA AACGGAGGCA GAAGAAACCG TGGACACCTT TGGCCAGGAA
    CGTGATGGCG TTACCAACGT GGTGCGATCC CCACTCTTTG GCCAGTTCCA GTTCAGCGTT
    AACTCCTGCG CCGAGAAGCC CAACAAGCAC GTCTGCACCA AGATCGTGAA TCAAAATGGC
    AAGAAGAAAA CGCTGACGCT GACCCGCCAG TGCTGCTATG GATATGGACG CCCCAGAAAC
    GCTGACTTCA CAACGCCCTG CGAGAAGATC GACATCAAGG ACGTGGAGGC CACGGCCAGT
    GACATGGGTG CCAAGCAGTT CCTGGAGAGT GCACGCACCG CCGGTGAGCT GGCCGATATG
    CTCGGAGCAG GCAGTGGCAA GAAGGTGACC CTCTTCGTGC CCAACGACGC CGCCTTCATG
    GAGTACCGTG GTCATCATCA GCAGGAGAAT AATGTGGAAG ATGCCAAGGC CGAGGCTTCC
    AAGCCATCCA TCTACAAACT GCACGCTGTT CTCGGCGAAG TTCAGCTGGA GGATGTGCCC
    AATGAGAAGC TTCTGCAGAC GGAGCTGCCT GACCAGAAGA TCCGCATCAA CAGCTACCAG
    CTGCCTGCGG CTTTGGGCCT GGAACCCTAC CGCTTTGCCG CCAACTGTGT GCCCATCGAG
    AAGCACGACA AGCTCTCGGA GCAGGCCCTT GTCCACACTC TGGGCGGAGT CCTGAAGCCG
    CTGACCAAGA ACCTTATGGA TATCATCAGG GAGCGTGCGG ATATGTCCAT TATGCGCACC
    GTTCTGGAGA AGACCAACCT GAGCGCCATG CTAGAGGATG ACAAGCCAGT GACCATCTTT
    GTGCCCACCG ATGCCGCCTT CGACAAGTTG GAACCTCATC TGCGTCGAGC CCTCAAGGAA
    GGTCGCGGTT GTGCCTCCAA CATCCTGAAG AACCATTTGC TGGACCTCAC CTTCTGCTCG
    CTGGCCACTG TTCCGGGTGC CAAGACGACG GCCTACAATC TACTGGGAGA ACCCCTGCTC
    CTCAACCGAA CCCACCGCGC TGCGAATCAG ACTGGACCGA CTCCCATCTA CATCAATAAC
    TTGGCCAAAA TCATTGATGC CGATATCATG GGCACCAATG GTGTGCTGCA CGTAATCGAC
    ACCATCCTGC CCACCGAATC GGCTCTGCCC ATGACCTCGC TGATGTCGCA AAAGAACCTG
    ACCATCTTCC AGCGATTGCT GGAGGCCTCC GGCTACGATG ATCAGTTCGA CGATCTGGAC
    AACGTGACCA TCTTTGCACC AACTGACAAG GCTCTGCAGA ATACGGAATG GGCTCGCATG
    CTCAAGGAAC AGCCGGAGCT GCTGAATCAT AACTTGGATC TGCTGGAGTT CCTTAACTAC
    CATGTGGTCA AGCCCATGAT CAAGACCTGT GACCTGTCGG AGAAGTCCCT GCCCACGGTT
    GCTGGATCTA GTGTGCGTCT GAACCTGTAC TCCACCCATG CTCTCTTCTC GGACGTGATG
    AACCGGGCAA CGGTAAACTG TGCCCGTTTG GTGCACTTCG ATGACGAGAG CTGCGGATCC
    GTTCTCCACC AGGTGGATCG TGCCTTGGCG CCTCCCAAGA ATAACATGCT TAAGCTGCTG
    GAGGCTAATC CCAACTACAG CAAGTTCCTG GAGCTGGTTC GTAAGGCTAA TCTTACACAG
    CTGCTGTCCA ACGATTCGAG GAGTCTCACC CTGCTGGTGC CAAAGAACGA TATCTTCGAG
    GAGCTGAACG AGTCCGGTGA GGGTTCAAAG CCCGCCGATC CCATGGCCTT GGTCAAAACC
    CATATCGTTG AGGATGTTGT CTGCTGTGCC GGCATTATCC CAACCAACTG GCCATTTGTC
    CGCTCCATCG AGTCCATCTC TGGCCAGCAT CTGCGTATCA CCCGCGATCG TCGTCCCAAG
    ATCGAGAACG CCGGCGTCAC CAAGTGCGAT GTGGTGGCCA CCAATGGAAT TCTCCATGAG
    ATCAACGACA TCATCGTGCC ACGCCCACAG CGCCAGCAAC AGCAGCGACC CCAGCTGATC
    CCGCCCGGAG CTGGCTATCA GCCACAGGGC GATTTCGATG TCTTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04618
    Clone ID Related Accession (Same CDS sequence) NM_169444.1 , NM_176470.1 , NM_176469.2 , NM_176468.1
    Accession Version NM_176470.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform P
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACAGCGGATA CGACACCGAG TTCGTGCCAC TGGAGCACCA GCAGCAGCCG
    CAGGGACGCC AGGAGACGGC GGCCACCGTT CCCGCTCAAA CCCCCAGCGG TGTGGAGCAG
    AAGCCGTTCA ATGTGGACAC CATCACCACG GATGTGAATT CCCCAAATCC GGCCATCTTC
    TTCCAGCAAT CGTTCCCCTT CTTTGGCAAT GAGTTCTTCA ATTCGTTCGG AGGCTTTGGC
    TTTGGAGCTG CCCAGGAGCC CTGGTGGAAG GGACCCAATG TGTGCACCGA AAAGGAGGAA
    GACGAGACTG TGGCCAGTGA AACGGAGGCA GAAGAAACCG TGGACACCTT TGGCCAGGAA
    CGTGATGGCG TTACCAACGT GGTGCGATCC CCACTCTTTG GCCAGTTCCA GTTCAGCGTT
    AACTCCTGCG CCGAGAAGCC CAACAAGCAC GTCTGCACCA AGATCGTGAA TCAAAATGGC
    AAGAAGAAAA CGCTGACGCT GACCCGCCAG TGCTGCTATG GATATGGACG CCCCAGAAAC
    GCTGACTTCA CAACGCCCTG CGAGAAGATC GACATCAAGG ACGTGGAGGC CACGGCCAGT
    GACATGGGTG CCAAGCAGTT CCTGGAGAGT GCACGCACCG CCGGTGAGCT GGCCGATATG
    CTCGGAGCAG GCAGTGGCAA GAAGGTGACC CTCTTCGTGC CCAACGACGC CGCCTTCATG
    GAGTACCGTG GTCATCATCA GCAGGAGAAT AATGTGGAAG ATGCCAAGGC CGAGGCTTCC
    AAGCCATCCA TCTACAAACT GCACGCTGTT CTCGGCGAAG TTCAGCTGGA GGATGTGCCC
    AATGAGAAGC TTCTGCAGAC GGAGCTGCCT GACCAGAAGA TCCGCATCAA CAGCTACCAG
    CTGCCTGCGG CTTTGGGCCT GGAACCCTAC CGCTTTGCCG CCAACTGTGT GCCCATCGAG
    AAGCACGACA AGCTCTCGGA GCAGGCCCTT GTCCACACTC TGGGCGGAGT CCTGAAGCCG
    CTGACCAAGA ACCTTATGGA TATCATCAGG GAGCGTGCGG ATATGTCCAT TATGCGCACC
    GTTCTGGAGA AGACCAACCT GAGCGCCATG CTAGAGGATG ACAAGCCAGT GACCATCTTT
    GTGCCCACCG ATGCCGCCTT CGACAAGTTG GAACCTCATC TGCGTCGAGC CCTCAAGGAA
    GGTCGCGGTT GTGCCTCCAA CATCCTGAAG AACCATTTGC TGGACCTCAC CTTCTGCTCG
    CTGGCCACTG TTCCGGGTGC CAAGACGACG GCCTACAATC TACTGGGAGA ACCCCTGCTC
    CTCAACCGAA CCCACCGCGC TGCGAATCAG ACTGGACCGA CTCCCATCTA CATCAATAAC
    TTGGCCAAAA TCATTGATGC CGATATCATG GGCACCAATG GTGTGCTGCA CGTAATCGAC
    ACCATCCTGC CCACCGAATC GGCTCTGCCC ATGACCTCGC TGATGTCGCA AAAGAACCTG
    ACCATCTTCC AGCGATTGCT GGAGGCCTCC GGCTACGATG ATCAGTTCGA CGATCTGGAC
    AACGTGACCA TCTTTGCACC AACTGACAAG GCTCTGCAGA ATACGGAATG GGCTCGCATG
    CTCAAGGAAC AGCCGGAGCT GCTGAATCAT AACTTGGATC TGCTGGAGTT CCTTAACTAC
    CATGTGGTCA AGCCCATGAT CAAGACCTGT GACCTGTCGG AGAAGTCCCT GCCCACGGTT
    GCTGGATCTA GTGTGCGTCT GAACCTGTAC TCCACCCATG CTCTCTTCTC GGACGTGATG
    AACCGGGCAA CGGTAAACTG TGCCCGTTTG GTGCACTTCG ATGACGAGAG CTGCGGATCC
    GTTCTCCACC AGGTGGATCG TGCCTTGGCG CCTCCCAAGA ATAACATGCT TAAGCTGCTG
    GAGGCTAATC CCAACTACAG CAAGTTCCTG GAGCTGGTTC GTAAGGCTAA TCTTACACAG
    CTGCTGTCCA ACGATTCGAG GAGTCTCACC CTGCTGGTGC CAAAGAACGA TATCTTCGAG
    GAGCTGAACG AGTCCGGTGA GGGTTCAAAG CCCGCCGATC CCATGGCCTT GGTCAAAACC
    CATATCGTTG AGGATGTTGT CTGCTGTGCC GGCATTATCC CAACCAACTG GCCATTTGTC
    CGCTCCATCG AGTCCATCTC TGGCCAGCAT CTGCGTATCA CCCGCGATCG TCGTCCCAAG
    ATCGAGAACG CCGGCGTCAC CAAGTGCGAT GTGGTGGCCA CCAATGGAAT TCTCCATGAG
    ATCAACGACA TCATCGTGCC ACGCCCACAG CGCCAGCAAC AGCAGCGACC CCAGCTGATC
    CCGCCCGGAG CTGGCTATCA GCCACAGGGC GATTTCGATG TCTTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788647.1
    CDS130..2580
    Translation

    Target ORF information:

    RefSeq Version NM_176470.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant P, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176470.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACAGCGGATA CGACACCGAG TTCGTGCCAC TGGAGCACCA GCAGCAGCCG
    CAGGGACGCC AGGAGACGGC GGCCACCGTT CCCGCTCAAA CCCCCAGCGG TGTGGAGCAG
    AAGCCGTTCA ATGTGGACAC CATCACCACG GATGTGAATT CCCCAAATCC GGCCATCTTC
    TTCCAGCAAT CGTTCCCCTT CTTTGGCAAT GAGTTCTTCA ATTCGTTCGG AGGCTTTGGC
    TTTGGAGCTG CCCAGGAGCC CTGGTGGAAG GGACCCAATG TGTGCACCGA AAAGGAGGAA
    GACGAGACTG TGGCCAGTGA AACGGAGGCA GAAGAAACCG TGGACACCTT TGGCCAGGAA
    CGTGATGGCG TTACCAACGT GGTGCGATCC CCACTCTTTG GCCAGTTCCA GTTCAGCGTT
    AACTCCTGCG CCGAGAAGCC CAACAAGCAC GTCTGCACCA AGATCGTGAA TCAAAATGGC
    AAGAAGAAAA CGCTGACGCT GACCCGCCAG TGCTGCTATG GATATGGACG CCCCAGAAAC
    GCTGACTTCA CAACGCCCTG CGAGAAGATC GACATCAAGG ACGTGGAGGC CACGGCCAGT
    GACATGGGTG CCAAGCAGTT CCTGGAGAGT GCACGCACCG CCGGTGAGCT GGCCGATATG
    CTCGGAGCAG GCAGTGGCAA GAAGGTGACC CTCTTCGTGC CCAACGACGC CGCCTTCATG
    GAGTACCGTG GTCATCATCA GCAGGAGAAT AATGTGGAAG ATGCCAAGGC CGAGGCTTCC
    AAGCCATCCA TCTACAAACT GCACGCTGTT CTCGGCGAAG TTCAGCTGGA GGATGTGCCC
    AATGAGAAGC TTCTGCAGAC GGAGCTGCCT GACCAGAAGA TCCGCATCAA CAGCTACCAG
    CTGCCTGCGG CTTTGGGCCT GGAACCCTAC CGCTTTGCCG CCAACTGTGT GCCCATCGAG
    AAGCACGACA AGCTCTCGGA GCAGGCCCTT GTCCACACTC TGGGCGGAGT CCTGAAGCCG
    CTGACCAAGA ACCTTATGGA TATCATCAGG GAGCGTGCGG ATATGTCCAT TATGCGCACC
    GTTCTGGAGA AGACCAACCT GAGCGCCATG CTAGAGGATG ACAAGCCAGT GACCATCTTT
    GTGCCCACCG ATGCCGCCTT CGACAAGTTG GAACCTCATC TGCGTCGAGC CCTCAAGGAA
    GGTCGCGGTT GTGCCTCCAA CATCCTGAAG AACCATTTGC TGGACCTCAC CTTCTGCTCG
    CTGGCCACTG TTCCGGGTGC CAAGACGACG GCCTACAATC TACTGGGAGA ACCCCTGCTC
    CTCAACCGAA CCCACCGCGC TGCGAATCAG ACTGGACCGA CTCCCATCTA CATCAATAAC
    TTGGCCAAAA TCATTGATGC CGATATCATG GGCACCAATG GTGTGCTGCA CGTAATCGAC
    ACCATCCTGC CCACCGAATC GGCTCTGCCC ATGACCTCGC TGATGTCGCA AAAGAACCTG
    ACCATCTTCC AGCGATTGCT GGAGGCCTCC GGCTACGATG ATCAGTTCGA CGATCTGGAC
    AACGTGACCA TCTTTGCACC AACTGACAAG GCTCTGCAGA ATACGGAATG GGCTCGCATG
    CTCAAGGAAC AGCCGGAGCT GCTGAATCAT AACTTGGATC TGCTGGAGTT CCTTAACTAC
    CATGTGGTCA AGCCCATGAT CAAGACCTGT GACCTGTCGG AGAAGTCCCT GCCCACGGTT
    GCTGGATCTA GTGTGCGTCT GAACCTGTAC TCCACCCATG CTCTCTTCTC GGACGTGATG
    AACCGGGCAA CGGTAAACTG TGCCCGTTTG GTGCACTTCG ATGACGAGAG CTGCGGATCC
    GTTCTCCACC AGGTGGATCG TGCCTTGGCG CCTCCCAAGA ATAACATGCT TAAGCTGCTG
    GAGGCTAATC CCAACTACAG CAAGTTCCTG GAGCTGGTTC GTAAGGCTAA TCTTACACAG
    CTGCTGTCCA ACGATTCGAG GAGTCTCACC CTGCTGGTGC CAAAGAACGA TATCTTCGAG
    GAGCTGAACG AGTCCGGTGA GGGTTCAAAG CCCGCCGATC CCATGGCCTT GGTCAAAACC
    CATATCGTTG AGGATGTTGT CTGCTGTGCC GGCATTATCC CAACCAACTG GCCATTTGTC
    CGCTCCATCG AGTCCATCTC TGGCCAGCAT CTGCGTATCA CCCGCGATCG TCGTCCCAAG
    ATCGAGAACG CCGGCGTCAC CAAGTGCGAT GTGGTGGCCA CCAATGGAAT TCTCCATGAG
    ATCAACGACA TCATCGTGCC ACGCCCACAG CGCCAGCAAC AGCAGCGACC CCAGCTGATC
    CCGCCCGGAG CTGGCTATCA GCCACAGGGC GATTTCGATG TCTTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04618
    Clone ID Related Accession (Same CDS sequence) NM_169444.1 , NM_176470.1 , NM_176469.2 , NM_176468.1
    Accession Version NM_176469.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176469.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACAGCGGATA CGACACCGAG TTCGTGCCAC TGGAGCACCA GCAGCAGCCG
    CAGGGACGCC AGGAGACGGC GGCCACCGTT CCCGCTCAAA CCCCCAGCGG TGTGGAGCAG
    AAGCCGTTCA ATGTGGACAC CATCACCACG GATGTGAATT CCCCAAATCC GGCCATCTTC
    TTCCAGCAAT CGTTCCCCTT CTTTGGCAAT GAGTTCTTCA ATTCGTTCGG AGGCTTTGGC
    TTTGGAGCTG CCCAGGAGCC CTGGTGGAAG GGACCCAATG TGTGCACCGA AAAGGAGGAA
    GACGAGACTG TGGCCAGTGA AACGGAGGCA GAAGAAACCG TGGACACCTT TGGCCAGGAA
    CGTGATGGCG TTACCAACGT GGTGCGATCC CCACTCTTTG GCCAGTTCCA GTTCAGCGTT
    AACTCCTGCG CCGAGAAGCC CAACAAGCAC GTCTGCACCA AGATCGTGAA TCAAAATGGC
    AAGAAGAAAA CGCTGACGCT GACCCGCCAG TGCTGCTATG GATATGGACG CCCCAGAAAC
    GCTGACTTCA CAACGCCCTG CGAGAAGATC GACATCAAGG ACGTGGAGGC CACGGCCAGT
    GACATGGGTG CCAAGCAGTT CCTGGAGAGT GCACGCACCG CCGGTGAGCT GGCCGATATG
    CTCGGAGCAG GCAGTGGCAA GAAGGTGACC CTCTTCGTGC CCAACGACGC CGCCTTCATG
    GAGTACCGTG GTCATCATCA GCAGGAGAAT AATGTGGAAG ATGCCAAGGC CGAGGCTTCC
    AAGCCATCCA TCTACAAACT GCACGCTGTT CTCGGCGAAG TTCAGCTGGA GGATGTGCCC
    AATGAGAAGC TTCTGCAGAC GGAGCTGCCT GACCAGAAGA TCCGCATCAA CAGCTACCAG
    CTGCCTGCGG CTTTGGGCCT GGAACCCTAC CGCTTTGCCG CCAACTGTGT GCCCATCGAG
    AAGCACGACA AGCTCTCGGA GCAGGCCCTT GTCCACACTC TGGGCGGAGT CCTGAAGCCG
    CTGACCAAGA ACCTTATGGA TATCATCAGG GAGCGTGCGG ATATGTCCAT TATGCGCACC
    GTTCTGGAGA AGACCAACCT GAGCGCCATG CTAGAGGATG ACAAGCCAGT GACCATCTTT
    GTGCCCACCG ATGCCGCCTT CGACAAGTTG GAACCTCATC TGCGTCGAGC CCTCAAGGAA
    GGTCGCGGTT GTGCCTCCAA CATCCTGAAG AACCATTTGC TGGACCTCAC CTTCTGCTCG
    CTGGCCACTG TTCCGGGTGC CAAGACGACG GCCTACAATC TACTGGGAGA ACCCCTGCTC
    CTCAACCGAA CCCACCGCGC TGCGAATCAG ACTGGACCGA CTCCCATCTA CATCAATAAC
    TTGGCCAAAA TCATTGATGC CGATATCATG GGCACCAATG GTGTGCTGCA CGTAATCGAC
    ACCATCCTGC CCACCGAATC GGCTCTGCCC ATGACCTCGC TGATGTCGCA AAAGAACCTG
    ACCATCTTCC AGCGATTGCT GGAGGCCTCC GGCTACGATG ATCAGTTCGA CGATCTGGAC
    AACGTGACCA TCTTTGCACC AACTGACAAG GCTCTGCAGA ATACGGAATG GGCTCGCATG
    CTCAAGGAAC AGCCGGAGCT GCTGAATCAT AACTTGGATC TGCTGGAGTT CCTTAACTAC
    CATGTGGTCA AGCCCATGAT CAAGACCTGT GACCTGTCGG AGAAGTCCCT GCCCACGGTT
    GCTGGATCTA GTGTGCGTCT GAACCTGTAC TCCACCCATG CTCTCTTCTC GGACGTGATG
    AACCGGGCAA CGGTAAACTG TGCCCGTTTG GTGCACTTCG ATGACGAGAG CTGCGGATCC
    GTTCTCCACC AGGTGGATCG TGCCTTGGCG CCTCCCAAGA ATAACATGCT TAAGCTGCTG
    GAGGCTAATC CCAACTACAG CAAGTTCCTG GAGCTGGTTC GTAAGGCTAA TCTTACACAG
    CTGCTGTCCA ACGATTCGAG GAGTCTCACC CTGCTGGTGC CAAAGAACGA TATCTTCGAG
    GAGCTGAACG AGTCCGGTGA GGGTTCAAAG CCCGCCGATC CCATGGCCTT GGTCAAAACC
    CATATCGTTG AGGATGTTGT CTGCTGTGCC GGCATTATCC CAACCAACTG GCCATTTGTC
    CGCTCCATCG AGTCCATCTC TGGCCAGCAT CTGCGTATCA CCCGCGATCG TCGTCCCAAG
    ATCGAGAACG CCGGCGTCAC CAAGTGCGAT GTGGTGGCCA CCAATGGAAT TCTCCATGAG
    ATCAACGACA TCATCGTGCC ACGCCCACAG CGCCAGCAAC AGCAGCGACC CCAGCTGATC
    CCGCCCGGAG CTGGCTATCA GCCACAGGGC GATTTCGATG TCTTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788646.1
    CDS225..2675
    Translation

    Target ORF information:

    RefSeq Version NM_176469.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176469.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACAGCGGATA CGACACCGAG TTCGTGCCAC TGGAGCACCA GCAGCAGCCG
    CAGGGACGCC AGGAGACGGC GGCCACCGTT CCCGCTCAAA CCCCCAGCGG TGTGGAGCAG
    AAGCCGTTCA ATGTGGACAC CATCACCACG GATGTGAATT CCCCAAATCC GGCCATCTTC
    TTCCAGCAAT CGTTCCCCTT CTTTGGCAAT GAGTTCTTCA ATTCGTTCGG AGGCTTTGGC
    TTTGGAGCTG CCCAGGAGCC CTGGTGGAAG GGACCCAATG TGTGCACCGA AAAGGAGGAA
    GACGAGACTG TGGCCAGTGA AACGGAGGCA GAAGAAACCG TGGACACCTT TGGCCAGGAA
    CGTGATGGCG TTACCAACGT GGTGCGATCC CCACTCTTTG GCCAGTTCCA GTTCAGCGTT
    AACTCCTGCG CCGAGAAGCC CAACAAGCAC GTCTGCACCA AGATCGTGAA TCAAAATGGC
    AAGAAGAAAA CGCTGACGCT GACCCGCCAG TGCTGCTATG GATATGGACG CCCCAGAAAC
    GCTGACTTCA CAACGCCCTG CGAGAAGATC GACATCAAGG ACGTGGAGGC CACGGCCAGT
    GACATGGGTG CCAAGCAGTT CCTGGAGAGT GCACGCACCG CCGGTGAGCT GGCCGATATG
    CTCGGAGCAG GCAGTGGCAA GAAGGTGACC CTCTTCGTGC CCAACGACGC CGCCTTCATG
    GAGTACCGTG GTCATCATCA GCAGGAGAAT AATGTGGAAG ATGCCAAGGC CGAGGCTTCC
    AAGCCATCCA TCTACAAACT GCACGCTGTT CTCGGCGAAG TTCAGCTGGA GGATGTGCCC
    AATGAGAAGC TTCTGCAGAC GGAGCTGCCT GACCAGAAGA TCCGCATCAA CAGCTACCAG
    CTGCCTGCGG CTTTGGGCCT GGAACCCTAC CGCTTTGCCG CCAACTGTGT GCCCATCGAG
    AAGCACGACA AGCTCTCGGA GCAGGCCCTT GTCCACACTC TGGGCGGAGT CCTGAAGCCG
    CTGACCAAGA ACCTTATGGA TATCATCAGG GAGCGTGCGG ATATGTCCAT TATGCGCACC
    GTTCTGGAGA AGACCAACCT GAGCGCCATG CTAGAGGATG ACAAGCCAGT GACCATCTTT
    GTGCCCACCG ATGCCGCCTT CGACAAGTTG GAACCTCATC TGCGTCGAGC CCTCAAGGAA
    GGTCGCGGTT GTGCCTCCAA CATCCTGAAG AACCATTTGC TGGACCTCAC CTTCTGCTCG
    CTGGCCACTG TTCCGGGTGC CAAGACGACG GCCTACAATC TACTGGGAGA ACCCCTGCTC
    CTCAACCGAA CCCACCGCGC TGCGAATCAG ACTGGACCGA CTCCCATCTA CATCAATAAC
    TTGGCCAAAA TCATTGATGC CGATATCATG GGCACCAATG GTGTGCTGCA CGTAATCGAC
    ACCATCCTGC CCACCGAATC GGCTCTGCCC ATGACCTCGC TGATGTCGCA AAAGAACCTG
    ACCATCTTCC AGCGATTGCT GGAGGCCTCC GGCTACGATG ATCAGTTCGA CGATCTGGAC
    AACGTGACCA TCTTTGCACC AACTGACAAG GCTCTGCAGA ATACGGAATG GGCTCGCATG
    CTCAAGGAAC AGCCGGAGCT GCTGAATCAT AACTTGGATC TGCTGGAGTT CCTTAACTAC
    CATGTGGTCA AGCCCATGAT CAAGACCTGT GACCTGTCGG AGAAGTCCCT GCCCACGGTT
    GCTGGATCTA GTGTGCGTCT GAACCTGTAC TCCACCCATG CTCTCTTCTC GGACGTGATG
    AACCGGGCAA CGGTAAACTG TGCCCGTTTG GTGCACTTCG ATGACGAGAG CTGCGGATCC
    GTTCTCCACC AGGTGGATCG TGCCTTGGCG CCTCCCAAGA ATAACATGCT TAAGCTGCTG
    GAGGCTAATC CCAACTACAG CAAGTTCCTG GAGCTGGTTC GTAAGGCTAA TCTTACACAG
    CTGCTGTCCA ACGATTCGAG GAGTCTCACC CTGCTGGTGC CAAAGAACGA TATCTTCGAG
    GAGCTGAACG AGTCCGGTGA GGGTTCAAAG CCCGCCGATC CCATGGCCTT GGTCAAAACC
    CATATCGTTG AGGATGTTGT CTGCTGTGCC GGCATTATCC CAACCAACTG GCCATTTGTC
    CGCTCCATCG AGTCCATCTC TGGCCAGCAT CTGCGTATCA CCCGCGATCG TCGTCCCAAG
    ATCGAGAACG CCGGCGTCAC CAAGTGCGAT GTGGTGGCCA CCAATGGAAT TCTCCATGAG
    ATCAACGACA TCATCGTGCC ACGCCCACAG CGCCAGCAAC AGCAGCGACC CCAGCTGATC
    CCGCCCGGAG CTGGCTATCA GCCACAGGGC GATTTCGATG TCTTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04618
    Clone ID Related Accession (Same CDS sequence) NM_169444.1 , NM_176470.1 , NM_176469.2 , NM_176468.1
    Accession Version NM_176468.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACAGCGGATA CGACACCGAG TTCGTGCCAC TGGAGCACCA GCAGCAGCCG
    CAGGGACGCC AGGAGACGGC GGCCACCGTT CCCGCTCAAA CCCCCAGCGG TGTGGAGCAG
    AAGCCGTTCA ATGTGGACAC CATCACCACG GATGTGAATT CCCCAAATCC GGCCATCTTC
    TTCCAGCAAT CGTTCCCCTT CTTTGGCAAT GAGTTCTTCA ATTCGTTCGG AGGCTTTGGC
    TTTGGAGCTG CCCAGGAGCC CTGGTGGAAG GGACCCAATG TGTGCACCGA AAAGGAGGAA
    GACGAGACTG TGGCCAGTGA AACGGAGGCA GAAGAAACCG TGGACACCTT TGGCCAGGAA
    CGTGATGGCG TTACCAACGT GGTGCGATCC CCACTCTTTG GCCAGTTCCA GTTCAGCGTT
    AACTCCTGCG CCGAGAAGCC CAACAAGCAC GTCTGCACCA AGATCGTGAA TCAAAATGGC
    AAGAAGAAAA CGCTGACGCT GACCCGCCAG TGCTGCTATG GATATGGACG CCCCAGAAAC
    GCTGACTTCA CAACGCCCTG CGAGAAGATC GACATCAAGG ACGTGGAGGC CACGGCCAGT
    GACATGGGTG CCAAGCAGTT CCTGGAGAGT GCACGCACCG CCGGTGAGCT GGCCGATATG
    CTCGGAGCAG GCAGTGGCAA GAAGGTGACC CTCTTCGTGC CCAACGACGC CGCCTTCATG
    GAGTACCGTG GTCATCATCA GCAGGAGAAT AATGTGGAAG ATGCCAAGGC CGAGGCTTCC
    AAGCCATCCA TCTACAAACT GCACGCTGTT CTCGGCGAAG TTCAGCTGGA GGATGTGCCC
    AATGAGAAGC TTCTGCAGAC GGAGCTGCCT GACCAGAAGA TCCGCATCAA CAGCTACCAG
    CTGCCTGCGG CTTTGGGCCT GGAACCCTAC CGCTTTGCCG CCAACTGTGT GCCCATCGAG
    AAGCACGACA AGCTCTCGGA GCAGGCCCTT GTCCACACTC TGGGCGGAGT CCTGAAGCCG
    CTGACCAAGA ACCTTATGGA TATCATCAGG GAGCGTGCGG ATATGTCCAT TATGCGCACC
    GTTCTGGAGA AGACCAACCT GAGCGCCATG CTAGAGGATG ACAAGCCAGT GACCATCTTT
    GTGCCCACCG ATGCCGCCTT CGACAAGTTG GAACCTCATC TGCGTCGAGC CCTCAAGGAA
    GGTCGCGGTT GTGCCTCCAA CATCCTGAAG AACCATTTGC TGGACCTCAC CTTCTGCTCG
    CTGGCCACTG TTCCGGGTGC CAAGACGACG GCCTACAATC TACTGGGAGA ACCCCTGCTC
    CTCAACCGAA CCCACCGCGC TGCGAATCAG ACTGGACCGA CTCCCATCTA CATCAATAAC
    TTGGCCAAAA TCATTGATGC CGATATCATG GGCACCAATG GTGTGCTGCA CGTAATCGAC
    ACCATCCTGC CCACCGAATC GGCTCTGCCC ATGACCTCGC TGATGTCGCA AAAGAACCTG
    ACCATCTTCC AGCGATTGCT GGAGGCCTCC GGCTACGATG ATCAGTTCGA CGATCTGGAC
    AACGTGACCA TCTTTGCACC AACTGACAAG GCTCTGCAGA ATACGGAATG GGCTCGCATG
    CTCAAGGAAC AGCCGGAGCT GCTGAATCAT AACTTGGATC TGCTGGAGTT CCTTAACTAC
    CATGTGGTCA AGCCCATGAT CAAGACCTGT GACCTGTCGG AGAAGTCCCT GCCCACGGTT
    GCTGGATCTA GTGTGCGTCT GAACCTGTAC TCCACCCATG CTCTCTTCTC GGACGTGATG
    AACCGGGCAA CGGTAAACTG TGCCCGTTTG GTGCACTTCG ATGACGAGAG CTGCGGATCC
    GTTCTCCACC AGGTGGATCG TGCCTTGGCG CCTCCCAAGA ATAACATGCT TAAGCTGCTG
    GAGGCTAATC CCAACTACAG CAAGTTCCTG GAGCTGGTTC GTAAGGCTAA TCTTACACAG
    CTGCTGTCCA ACGATTCGAG GAGTCTCACC CTGCTGGTGC CAAAGAACGA TATCTTCGAG
    GAGCTGAACG AGTCCGGTGA GGGTTCAAAG CCCGCCGATC CCATGGCCTT GGTCAAAACC
    CATATCGTTG AGGATGTTGT CTGCTGTGCC GGCATTATCC CAACCAACTG GCCATTTGTC
    CGCTCCATCG AGTCCATCTC TGGCCAGCAT CTGCGTATCA CCCGCGATCG TCGTCCCAAG
    ATCGAGAACG CCGGCGTCAC CAAGTGCGAT GTGGTGGCCA CCAATGGAAT TCTCCATGAG
    ATCAACGACA TCATCGTGCC ACGCCCACAG CGCCAGCAAC AGCAGCGACC CCAGCTGATC
    CCGCCCGGAG CTGGCTATCA GCCACAGGGC GATTTCGATG TCTTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788645.1
    CDS504..2954
    Translation

    Target ORF information:

    RefSeq Version NM_176468.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176468.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACAGCGGATA CGACACCGAG TTCGTGCCAC TGGAGCACCA GCAGCAGCCG
    CAGGGACGCC AGGAGACGGC GGCCACCGTT CCCGCTCAAA CCCCCAGCGG TGTGGAGCAG
    AAGCCGTTCA ATGTGGACAC CATCACCACG GATGTGAATT CCCCAAATCC GGCCATCTTC
    TTCCAGCAAT CGTTCCCCTT CTTTGGCAAT GAGTTCTTCA ATTCGTTCGG AGGCTTTGGC
    TTTGGAGCTG CCCAGGAGCC CTGGTGGAAG GGACCCAATG TGTGCACCGA AAAGGAGGAA
    GACGAGACTG TGGCCAGTGA AACGGAGGCA GAAGAAACCG TGGACACCTT TGGCCAGGAA
    CGTGATGGCG TTACCAACGT GGTGCGATCC CCACTCTTTG GCCAGTTCCA GTTCAGCGTT
    AACTCCTGCG CCGAGAAGCC CAACAAGCAC GTCTGCACCA AGATCGTGAA TCAAAATGGC
    AAGAAGAAAA CGCTGACGCT GACCCGCCAG TGCTGCTATG GATATGGACG CCCCAGAAAC
    GCTGACTTCA CAACGCCCTG CGAGAAGATC GACATCAAGG ACGTGGAGGC CACGGCCAGT
    GACATGGGTG CCAAGCAGTT CCTGGAGAGT GCACGCACCG CCGGTGAGCT GGCCGATATG
    CTCGGAGCAG GCAGTGGCAA GAAGGTGACC CTCTTCGTGC CCAACGACGC CGCCTTCATG
    GAGTACCGTG GTCATCATCA GCAGGAGAAT AATGTGGAAG ATGCCAAGGC CGAGGCTTCC
    AAGCCATCCA TCTACAAACT GCACGCTGTT CTCGGCGAAG TTCAGCTGGA GGATGTGCCC
    AATGAGAAGC TTCTGCAGAC GGAGCTGCCT GACCAGAAGA TCCGCATCAA CAGCTACCAG
    CTGCCTGCGG CTTTGGGCCT GGAACCCTAC CGCTTTGCCG CCAACTGTGT GCCCATCGAG
    AAGCACGACA AGCTCTCGGA GCAGGCCCTT GTCCACACTC TGGGCGGAGT CCTGAAGCCG
    CTGACCAAGA ACCTTATGGA TATCATCAGG GAGCGTGCGG ATATGTCCAT TATGCGCACC
    GTTCTGGAGA AGACCAACCT GAGCGCCATG CTAGAGGATG ACAAGCCAGT GACCATCTTT
    GTGCCCACCG ATGCCGCCTT CGACAAGTTG GAACCTCATC TGCGTCGAGC CCTCAAGGAA
    GGTCGCGGTT GTGCCTCCAA CATCCTGAAG AACCATTTGC TGGACCTCAC CTTCTGCTCG
    CTGGCCACTG TTCCGGGTGC CAAGACGACG GCCTACAATC TACTGGGAGA ACCCCTGCTC
    CTCAACCGAA CCCACCGCGC TGCGAATCAG ACTGGACCGA CTCCCATCTA CATCAATAAC
    TTGGCCAAAA TCATTGATGC CGATATCATG GGCACCAATG GTGTGCTGCA CGTAATCGAC
    ACCATCCTGC CCACCGAATC GGCTCTGCCC ATGACCTCGC TGATGTCGCA AAAGAACCTG
    ACCATCTTCC AGCGATTGCT GGAGGCCTCC GGCTACGATG ATCAGTTCGA CGATCTGGAC
    AACGTGACCA TCTTTGCACC AACTGACAAG GCTCTGCAGA ATACGGAATG GGCTCGCATG
    CTCAAGGAAC AGCCGGAGCT GCTGAATCAT AACTTGGATC TGCTGGAGTT CCTTAACTAC
    CATGTGGTCA AGCCCATGAT CAAGACCTGT GACCTGTCGG AGAAGTCCCT GCCCACGGTT
    GCTGGATCTA GTGTGCGTCT GAACCTGTAC TCCACCCATG CTCTCTTCTC GGACGTGATG
    AACCGGGCAA CGGTAAACTG TGCCCGTTTG GTGCACTTCG ATGACGAGAG CTGCGGATCC
    GTTCTCCACC AGGTGGATCG TGCCTTGGCG CCTCCCAAGA ATAACATGCT TAAGCTGCTG
    GAGGCTAATC CCAACTACAG CAAGTTCCTG GAGCTGGTTC GTAAGGCTAA TCTTACACAG
    CTGCTGTCCA ACGATTCGAG GAGTCTCACC CTGCTGGTGC CAAAGAACGA TATCTTCGAG
    GAGCTGAACG AGTCCGGTGA GGGTTCAAAG CCCGCCGATC CCATGGCCTT GGTCAAAACC
    CATATCGTTG AGGATGTTGT CTGCTGTGCC GGCATTATCC CAACCAACTG GCCATTTGTC
    CGCTCCATCG AGTCCATCTC TGGCCAGCAT CTGCGTATCA CCCGCGATCG TCGTCCCAAG
    ATCGAGAACG CCGGCGTCAC CAAGTGCGAT GTGGTGGCCA CCAATGGAAT TCTCCATGAG
    ATCAACGACA TCATCGTGCC ACGCCCACAG CGCCAGCAAC AGCAGCGACC CCAGCTGATC
    CCGCCCGGAG CTGGCTATCA GCCACAGGGC GATTTCGATG TCTTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04619
    Clone ID Related Accession (Same CDS sequence) NM_079600.3 , NM_176471.1 , NM_176472.1
    Accession Version NM_079600.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079600.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACTTTATGCC ACATTTAACC CACCCATTGC CGGCGCATCG CAATCTGTTC
    GCGCCCCTGC CCTTCGCACC ACTCGCACCG CTCGAGCCCC AAGATCCACT GCATGCCCAG
    GCCTCGCAGC GGATGGCGAG CGTCTGGAAC GGACCCGCCC CGTTGGCGCC GCAACTCCAC
    TCATCTCACC TGTTGCCCCT CTTCAGCAGC GGATACGACA CCGAGTTCGT GCCACTGGAG
    CACCAGCAGC AGCCGCAGGG ACGCCAGGAG ACGGCGGCCA CCGTTCCCGC TCAAACCCCC
    AGCGGTGTGG AGCAGAAGCC GTTCAATGTG GACACCATCA CCACGGATGT GAATTCCCCA
    AATCCGGCCA TCTTCTTCCA GCAATCGTTC CCCTTCTTTG GCAATGAGTT CTTCAATTCG
    TTCGGAGGCT TTGGCTTTGG AGCTGCCCAG GAGCCCTGGT GGAAGGGACC CAATGTGTGC
    ACCGAAAAGG AGGAAGACGA GACTGTGGCC AGTGAAACGG AGGCAGAAGA AACCGTGGAC
    ACCTTTGGCC AGGAACGTGA TGGCGTTACC AACGTGGTGC GATCCCCACT CTTTGGCCAG
    TTCCAGTTCA GCGTTAACTC CTGCGCCGAG AAGCCCAACA AGCACGTCTG CACCAAGATC
    GTGAATCAAA ATGGCAAGAA GAAAACGCTG ACGCTGACCC GCCAGTGCTG CTATGGATAT
    GGACGCCCCA GAAACGCTGA CTTCACAACG CCCTGCGAGA AGATCGACAT CAAGGACGTG
    GAGGCCACGG CCAGTGACAT GGGTGCCAAG CAGTTCCTGG AGAGTGCACG CACCGCCGGT
    GAGCTGGCCG ATATGCTCGG AGCAGGCAGT GGCAAGAAGG TGACCCTCTT CGTGCCCAAC
    GACGCCGCCT TCATGGAGTA CCGTGGTCAT CATCAGCAGG AGAATAATGT GGAAGATGCC
    AAGGCCGAGG CTTCCAAGCC ATCCATCTAC AAACTGCACG CTGTTCTCGG CGAAGTTCAG
    CTGGAGGATG TGCCCAATGA GAAGCTTCTG CAGACGGAGC TGCCTGACCA GAAGATCCGC
    ATCAACAGCT ACCAGCTGCC TGCGGCTTTG GGCCTGGAAC CCTACCGCTT TGCCGCCAAC
    TGTGTGCCCA TCGAGAAGCA CGACAAGCTC TCGGAGCAGG CCCTTGTCCA CACTCTGGGC
    GGAGTCCTGA AGCCGCTGAC CAAGAACCTT ATGGATATCA TCAGGGAGCG TGCGGATATG
    TCCATTATGC GCACCGTTCT GGAGAAGACC AACCTGAGCG CCATGCTAGA GGATGACAAG
    CCAGTGACCA TCTTTGTGCC CACCGATGCC GCCTTCGACA AGTTGGAACC TCATCTGCGT
    CGAGCCCTCA AGGAAGGTCG CGGTTGTGCC TCCAACATCC TGAAGAACCA TTTGCTGGAC
    CTCACCTTCT GCTCGCTGGC CACTGTTCCG GGTGCCAAGA CGACGGCCTA CAATCTACTG
    GGAGAACCCC TGCTCCTCAA CCGAACCCAC CGCGCTGCGA ATCAGACTGG ACCGACTCCC
    ATCTACATCA ATAACTTGGC CAAAATCATT GATGCCGATA TCATGGGCAC CAATGGTGTG
    CTGCACGTAA TCGACACCAT CCTGCCCACC GAATCGGCTC TGCCCATGAC CTCGCTGATG
    TCGCAAAAGA ACCTGACCAT CTTCCAGCGA TTGCTGGAGG CCTCCGGCTA CGATGATCAG
    TTCGACGATC TGGACAACGT GACCATCTTT GCACCAACTG ACAAGGCTCT GCAGAATACG
    GAATGGGCTC GCATGCTCAA GGAACAGCCG GAGCTGCTGA ATCATAACTT GGATCTGCTG
    GAGTTCCTTA ACTACCATGT GGTCAAGCCC ATGATCAAGA CCTGTGACCT GTCGGAGAAG
    TCCCTGCCCA CGGTTGCTGG ATCTAGTGTG CGTCTGAACC TGTACTCCAC CCATGCTCTC
    TTCTCGGACG TGATGAACCG GGCAACGGTA AACTGTGCCC GTTTGGTGCA CTTCGATGAC
    GAGAGCTGCG GATCCGTTCT CCACCAGGTG GATCGTGCCT TGGCGCCTCC CAAGAATAAC
    ATGCTTAAGC TGCTGGAGGC TAATCCCAAC TACAGCAAGT TCCTGGAGCT GGTTCGTAAG
    GCTAATCTTA CACAGCTGCT GTCCAACGAT TCGAGGAGTC TCACCCTGCT GGTGCCAAAG
    AACGATATCT TCGAGGAGCT GAACGAGTCC GGTGAGGGTT CAAAGCCCGC CGATCCCATG
    GCCTTGGTCA AAACCCATAT CGTTGAGGAT GTTGTCTGCT GTGCCGGCAT TATCCCAACC
    AACTGGCCAT TTGTCCGCTC CATCGAGTCC ATCTCTGGCC AGCATCTGCG TATCACCCGC
    GATCGTCGTC CCAAGATCGA GAACGCCGGC GTCACCAAGT GCGATGTGGT GGCCACCAAT
    GGAATTCTCC ATGAGATCAA CGACATCATC GTGCCACGCC CACAGCGCCA GCAACAGCAG
    CGACCCCAGC TGATCCCGCC CGGAGCTGGC TATCAGCCAC AGGGCGATTT CGATGTCTTC
    TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_524324.2
    CDS225..2870
    Translation

    Target ORF information:

    RefSeq Version NM_079600.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_079600.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACTTTATGCC ACATTTAACC CACCCATTGC CGGCGCATCG CAATCTGTTC
    GCGCCCCTGC CCTTCGCACC ACTCGCACCG CTCGAGCCCC AAGATCCACT GCATGCCCAG
    GCCTCGCAGC GGATGGCGAG CGTCTGGAAC GGACCCGCCC CGTTGGCGCC GCAACTCCAC
    TCATCTCACC TGTTGCCCCT CTTCAGCAGC GGATACGACA CCGAGTTCGT GCCACTGGAG
    CACCAGCAGC AGCCGCAGGG ACGCCAGGAG ACGGCGGCCA CCGTTCCCGC TCAAACCCCC
    AGCGGTGTGG AGCAGAAGCC GTTCAATGTG GACACCATCA CCACGGATGT GAATTCCCCA
    AATCCGGCCA TCTTCTTCCA GCAATCGTTC CCCTTCTTTG GCAATGAGTT CTTCAATTCG
    TTCGGAGGCT TTGGCTTTGG AGCTGCCCAG GAGCCCTGGT GGAAGGGACC CAATGTGTGC
    ACCGAAAAGG AGGAAGACGA GACTGTGGCC AGTGAAACGG AGGCAGAAGA AACCGTGGAC
    ACCTTTGGCC AGGAACGTGA TGGCGTTACC AACGTGGTGC GATCCCCACT CTTTGGCCAG
    TTCCAGTTCA GCGTTAACTC CTGCGCCGAG AAGCCCAACA AGCACGTCTG CACCAAGATC
    GTGAATCAAA ATGGCAAGAA GAAAACGCTG ACGCTGACCC GCCAGTGCTG CTATGGATAT
    GGACGCCCCA GAAACGCTGA CTTCACAACG CCCTGCGAGA AGATCGACAT CAAGGACGTG
    GAGGCCACGG CCAGTGACAT GGGTGCCAAG CAGTTCCTGG AGAGTGCACG CACCGCCGGT
    GAGCTGGCCG ATATGCTCGG AGCAGGCAGT GGCAAGAAGG TGACCCTCTT CGTGCCCAAC
    GACGCCGCCT TCATGGAGTA CCGTGGTCAT CATCAGCAGG AGAATAATGT GGAAGATGCC
    AAGGCCGAGG CTTCCAAGCC ATCCATCTAC AAACTGCACG CTGTTCTCGG CGAAGTTCAG
    CTGGAGGATG TGCCCAATGA GAAGCTTCTG CAGACGGAGC TGCCTGACCA GAAGATCCGC
    ATCAACAGCT ACCAGCTGCC TGCGGCTTTG GGCCTGGAAC CCTACCGCTT TGCCGCCAAC
    TGTGTGCCCA TCGAGAAGCA CGACAAGCTC TCGGAGCAGG CCCTTGTCCA CACTCTGGGC
    GGAGTCCTGA AGCCGCTGAC CAAGAACCTT ATGGATATCA TCAGGGAGCG TGCGGATATG
    TCCATTATGC GCACCGTTCT GGAGAAGACC AACCTGAGCG CCATGCTAGA GGATGACAAG
    CCAGTGACCA TCTTTGTGCC CACCGATGCC GCCTTCGACA AGTTGGAACC TCATCTGCGT
    CGAGCCCTCA AGGAAGGTCG CGGTTGTGCC TCCAACATCC TGAAGAACCA TTTGCTGGAC
    CTCACCTTCT GCTCGCTGGC CACTGTTCCG GGTGCCAAGA CGACGGCCTA CAATCTACTG
    GGAGAACCCC TGCTCCTCAA CCGAACCCAC CGCGCTGCGA ATCAGACTGG ACCGACTCCC
    ATCTACATCA ATAACTTGGC CAAAATCATT GATGCCGATA TCATGGGCAC CAATGGTGTG
    CTGCACGTAA TCGACACCAT CCTGCCCACC GAATCGGCTC TGCCCATGAC CTCGCTGATG
    TCGCAAAAGA ACCTGACCAT CTTCCAGCGA TTGCTGGAGG CCTCCGGCTA CGATGATCAG
    TTCGACGATC TGGACAACGT GACCATCTTT GCACCAACTG ACAAGGCTCT GCAGAATACG
    GAATGGGCTC GCATGCTCAA GGAACAGCCG GAGCTGCTGA ATCATAACTT GGATCTGCTG
    GAGTTCCTTA ACTACCATGT GGTCAAGCCC ATGATCAAGA CCTGTGACCT GTCGGAGAAG
    TCCCTGCCCA CGGTTGCTGG ATCTAGTGTG CGTCTGAACC TGTACTCCAC CCATGCTCTC
    TTCTCGGACG TGATGAACCG GGCAACGGTA AACTGTGCCC GTTTGGTGCA CTTCGATGAC
    GAGAGCTGCG GATCCGTTCT CCACCAGGTG GATCGTGCCT TGGCGCCTCC CAAGAATAAC
    ATGCTTAAGC TGCTGGAGGC TAATCCCAAC TACAGCAAGT TCCTGGAGCT GGTTCGTAAG
    GCTAATCTTA CACAGCTGCT GTCCAACGAT TCGAGGAGTC TCACCCTGCT GGTGCCAAAG
    AACGATATCT TCGAGGAGCT GAACGAGTCC GGTGAGGGTT CAAAGCCCGC CGATCCCATG
    GCCTTGGTCA AAACCCATAT CGTTGAGGAT GTTGTCTGCT GTGCCGGCAT TATCCCAACC
    AACTGGCCAT TTGTCCGCTC CATCGAGTCC ATCTCTGGCC AGCATCTGCG TATCACCCGC
    GATCGTCGTC CCAAGATCGA GAACGCCGGC GTCACCAAGT GCGATGTGGT GGCCACCAAT
    GGAATTCTCC ATGAGATCAA CGACATCATC GTGCCACGCC CACAGCGCCA GCAACAGCAG
    CGACCCCAGC TGATCCCGCC CGGAGCTGGC TATCAGCCAC AGGGCGATTT CGATGTCTTC
    TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04619
    Clone ID Related Accession (Same CDS sequence) NM_079600.3 , NM_176471.1 , NM_176472.1
    Accession Version NM_176471.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACTTTATGCC ACATTTAACC CACCCATTGC CGGCGCATCG CAATCTGTTC
    GCGCCCCTGC CCTTCGCACC ACTCGCACCG CTCGAGCCCC AAGATCCACT GCATGCCCAG
    GCCTCGCAGC GGATGGCGAG CGTCTGGAAC GGACCCGCCC CGTTGGCGCC GCAACTCCAC
    TCATCTCACC TGTTGCCCCT CTTCAGCAGC GGATACGACA CCGAGTTCGT GCCACTGGAG
    CACCAGCAGC AGCCGCAGGG ACGCCAGGAG ACGGCGGCCA CCGTTCCCGC TCAAACCCCC
    AGCGGTGTGG AGCAGAAGCC GTTCAATGTG GACACCATCA CCACGGATGT GAATTCCCCA
    AATCCGGCCA TCTTCTTCCA GCAATCGTTC CCCTTCTTTG GCAATGAGTT CTTCAATTCG
    TTCGGAGGCT TTGGCTTTGG AGCTGCCCAG GAGCCCTGGT GGAAGGGACC CAATGTGTGC
    ACCGAAAAGG AGGAAGACGA GACTGTGGCC AGTGAAACGG AGGCAGAAGA AACCGTGGAC
    ACCTTTGGCC AGGAACGTGA TGGCGTTACC AACGTGGTGC GATCCCCACT CTTTGGCCAG
    TTCCAGTTCA GCGTTAACTC CTGCGCCGAG AAGCCCAACA AGCACGTCTG CACCAAGATC
    GTGAATCAAA ATGGCAAGAA GAAAACGCTG ACGCTGACCC GCCAGTGCTG CTATGGATAT
    GGACGCCCCA GAAACGCTGA CTTCACAACG CCCTGCGAGA AGATCGACAT CAAGGACGTG
    GAGGCCACGG CCAGTGACAT GGGTGCCAAG CAGTTCCTGG AGAGTGCACG CACCGCCGGT
    GAGCTGGCCG ATATGCTCGG AGCAGGCAGT GGCAAGAAGG TGACCCTCTT CGTGCCCAAC
    GACGCCGCCT TCATGGAGTA CCGTGGTCAT CATCAGCAGG AGAATAATGT GGAAGATGCC
    AAGGCCGAGG CTTCCAAGCC ATCCATCTAC AAACTGCACG CTGTTCTCGG CGAAGTTCAG
    CTGGAGGATG TGCCCAATGA GAAGCTTCTG CAGACGGAGC TGCCTGACCA GAAGATCCGC
    ATCAACAGCT ACCAGCTGCC TGCGGCTTTG GGCCTGGAAC CCTACCGCTT TGCCGCCAAC
    TGTGTGCCCA TCGAGAAGCA CGACAAGCTC TCGGAGCAGG CCCTTGTCCA CACTCTGGGC
    GGAGTCCTGA AGCCGCTGAC CAAGAACCTT ATGGATATCA TCAGGGAGCG TGCGGATATG
    TCCATTATGC GCACCGTTCT GGAGAAGACC AACCTGAGCG CCATGCTAGA GGATGACAAG
    CCAGTGACCA TCTTTGTGCC CACCGATGCC GCCTTCGACA AGTTGGAACC TCATCTGCGT
    CGAGCCCTCA AGGAAGGTCG CGGTTGTGCC TCCAACATCC TGAAGAACCA TTTGCTGGAC
    CTCACCTTCT GCTCGCTGGC CACTGTTCCG GGTGCCAAGA CGACGGCCTA CAATCTACTG
    GGAGAACCCC TGCTCCTCAA CCGAACCCAC CGCGCTGCGA ATCAGACTGG ACCGACTCCC
    ATCTACATCA ATAACTTGGC CAAAATCATT GATGCCGATA TCATGGGCAC CAATGGTGTG
    CTGCACGTAA TCGACACCAT CCTGCCCACC GAATCGGCTC TGCCCATGAC CTCGCTGATG
    TCGCAAAAGA ACCTGACCAT CTTCCAGCGA TTGCTGGAGG CCTCCGGCTA CGATGATCAG
    TTCGACGATC TGGACAACGT GACCATCTTT GCACCAACTG ACAAGGCTCT GCAGAATACG
    GAATGGGCTC GCATGCTCAA GGAACAGCCG GAGCTGCTGA ATCATAACTT GGATCTGCTG
    GAGTTCCTTA ACTACCATGT GGTCAAGCCC ATGATCAAGA CCTGTGACCT GTCGGAGAAG
    TCCCTGCCCA CGGTTGCTGG ATCTAGTGTG CGTCTGAACC TGTACTCCAC CCATGCTCTC
    TTCTCGGACG TGATGAACCG GGCAACGGTA AACTGTGCCC GTTTGGTGCA CTTCGATGAC
    GAGAGCTGCG GATCCGTTCT CCACCAGGTG GATCGTGCCT TGGCGCCTCC CAAGAATAAC
    ATGCTTAAGC TGCTGGAGGC TAATCCCAAC TACAGCAAGT TCCTGGAGCT GGTTCGTAAG
    GCTAATCTTA CACAGCTGCT GTCCAACGAT TCGAGGAGTC TCACCCTGCT GGTGCCAAAG
    AACGATATCT TCGAGGAGCT GAACGAGTCC GGTGAGGGTT CAAAGCCCGC CGATCCCATG
    GCCTTGGTCA AAACCCATAT CGTTGAGGAT GTTGTCTGCT GTGCCGGCAT TATCCCAACC
    AACTGGCCAT TTGTCCGCTC CATCGAGTCC ATCTCTGGCC AGCATCTGCG TATCACCCGC
    GATCGTCGTC CCAAGATCGA GAACGCCGGC GTCACCAAGT GCGATGTGGT GGCCACCAAT
    GGAATTCTCC ATGAGATCAA CGACATCATC GTGCCACGCC CACAGCGCCA GCAACAGCAG
    CGACCCCAGC TGATCCCGCC CGGAGCTGGC TATCAGCCAC AGGGCGATTT CGATGTCTTC
    TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788648.1
    CDS108..2753
    Translation

    Target ORF information:

    RefSeq Version NM_176471.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176471.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACTTTATGCC ACATTTAACC CACCCATTGC CGGCGCATCG CAATCTGTTC
    GCGCCCCTGC CCTTCGCACC ACTCGCACCG CTCGAGCCCC AAGATCCACT GCATGCCCAG
    GCCTCGCAGC GGATGGCGAG CGTCTGGAAC GGACCCGCCC CGTTGGCGCC GCAACTCCAC
    TCATCTCACC TGTTGCCCCT CTTCAGCAGC GGATACGACA CCGAGTTCGT GCCACTGGAG
    CACCAGCAGC AGCCGCAGGG ACGCCAGGAG ACGGCGGCCA CCGTTCCCGC TCAAACCCCC
    AGCGGTGTGG AGCAGAAGCC GTTCAATGTG GACACCATCA CCACGGATGT GAATTCCCCA
    AATCCGGCCA TCTTCTTCCA GCAATCGTTC CCCTTCTTTG GCAATGAGTT CTTCAATTCG
    TTCGGAGGCT TTGGCTTTGG AGCTGCCCAG GAGCCCTGGT GGAAGGGACC CAATGTGTGC
    ACCGAAAAGG AGGAAGACGA GACTGTGGCC AGTGAAACGG AGGCAGAAGA AACCGTGGAC
    ACCTTTGGCC AGGAACGTGA TGGCGTTACC AACGTGGTGC GATCCCCACT CTTTGGCCAG
    TTCCAGTTCA GCGTTAACTC CTGCGCCGAG AAGCCCAACA AGCACGTCTG CACCAAGATC
    GTGAATCAAA ATGGCAAGAA GAAAACGCTG ACGCTGACCC GCCAGTGCTG CTATGGATAT
    GGACGCCCCA GAAACGCTGA CTTCACAACG CCCTGCGAGA AGATCGACAT CAAGGACGTG
    GAGGCCACGG CCAGTGACAT GGGTGCCAAG CAGTTCCTGG AGAGTGCACG CACCGCCGGT
    GAGCTGGCCG ATATGCTCGG AGCAGGCAGT GGCAAGAAGG TGACCCTCTT CGTGCCCAAC
    GACGCCGCCT TCATGGAGTA CCGTGGTCAT CATCAGCAGG AGAATAATGT GGAAGATGCC
    AAGGCCGAGG CTTCCAAGCC ATCCATCTAC AAACTGCACG CTGTTCTCGG CGAAGTTCAG
    CTGGAGGATG TGCCCAATGA GAAGCTTCTG CAGACGGAGC TGCCTGACCA GAAGATCCGC
    ATCAACAGCT ACCAGCTGCC TGCGGCTTTG GGCCTGGAAC CCTACCGCTT TGCCGCCAAC
    TGTGTGCCCA TCGAGAAGCA CGACAAGCTC TCGGAGCAGG CCCTTGTCCA CACTCTGGGC
    GGAGTCCTGA AGCCGCTGAC CAAGAACCTT ATGGATATCA TCAGGGAGCG TGCGGATATG
    TCCATTATGC GCACCGTTCT GGAGAAGACC AACCTGAGCG CCATGCTAGA GGATGACAAG
    CCAGTGACCA TCTTTGTGCC CACCGATGCC GCCTTCGACA AGTTGGAACC TCATCTGCGT
    CGAGCCCTCA AGGAAGGTCG CGGTTGTGCC TCCAACATCC TGAAGAACCA TTTGCTGGAC
    CTCACCTTCT GCTCGCTGGC CACTGTTCCG GGTGCCAAGA CGACGGCCTA CAATCTACTG
    GGAGAACCCC TGCTCCTCAA CCGAACCCAC CGCGCTGCGA ATCAGACTGG ACCGACTCCC
    ATCTACATCA ATAACTTGGC CAAAATCATT GATGCCGATA TCATGGGCAC CAATGGTGTG
    CTGCACGTAA TCGACACCAT CCTGCCCACC GAATCGGCTC TGCCCATGAC CTCGCTGATG
    TCGCAAAAGA ACCTGACCAT CTTCCAGCGA TTGCTGGAGG CCTCCGGCTA CGATGATCAG
    TTCGACGATC TGGACAACGT GACCATCTTT GCACCAACTG ACAAGGCTCT GCAGAATACG
    GAATGGGCTC GCATGCTCAA GGAACAGCCG GAGCTGCTGA ATCATAACTT GGATCTGCTG
    GAGTTCCTTA ACTACCATGT GGTCAAGCCC ATGATCAAGA CCTGTGACCT GTCGGAGAAG
    TCCCTGCCCA CGGTTGCTGG ATCTAGTGTG CGTCTGAACC TGTACTCCAC CCATGCTCTC
    TTCTCGGACG TGATGAACCG GGCAACGGTA AACTGTGCCC GTTTGGTGCA CTTCGATGAC
    GAGAGCTGCG GATCCGTTCT CCACCAGGTG GATCGTGCCT TGGCGCCTCC CAAGAATAAC
    ATGCTTAAGC TGCTGGAGGC TAATCCCAAC TACAGCAAGT TCCTGGAGCT GGTTCGTAAG
    GCTAATCTTA CACAGCTGCT GTCCAACGAT TCGAGGAGTC TCACCCTGCT GGTGCCAAAG
    AACGATATCT TCGAGGAGCT GAACGAGTCC GGTGAGGGTT CAAAGCCCGC CGATCCCATG
    GCCTTGGTCA AAACCCATAT CGTTGAGGAT GTTGTCTGCT GTGCCGGCAT TATCCCAACC
    AACTGGCCAT TTGTCCGCTC CATCGAGTCC ATCTCTGGCC AGCATCTGCG TATCACCCGC
    GATCGTCGTC CCAAGATCGA GAACGCCGGC GTCACCAAGT GCGATGTGGT GGCCACCAAT
    GGAATTCTCC ATGAGATCAA CGACATCATC GTGCCACGCC CACAGCGCCA GCAACAGCAG
    CGACCCCAGC TGATCCCGCC CGGAGCTGGC TATCAGCCAC AGGGCGATTT CGATGTCTTC
    TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04619
    Clone ID Related Accession (Same CDS sequence) NM_079600.3 , NM_176471.1 , NM_176472.1
    Accession Version NM_176472.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACTTTATGCC ACATTTAACC CACCCATTGC CGGCGCATCG CAATCTGTTC
    GCGCCCCTGC CCTTCGCACC ACTCGCACCG CTCGAGCCCC AAGATCCACT GCATGCCCAG
    GCCTCGCAGC GGATGGCGAG CGTCTGGAAC GGACCCGCCC CGTTGGCGCC GCAACTCCAC
    TCATCTCACC TGTTGCCCCT CTTCAGCAGC GGATACGACA CCGAGTTCGT GCCACTGGAG
    CACCAGCAGC AGCCGCAGGG ACGCCAGGAG ACGGCGGCCA CCGTTCCCGC TCAAACCCCC
    AGCGGTGTGG AGCAGAAGCC GTTCAATGTG GACACCATCA CCACGGATGT GAATTCCCCA
    AATCCGGCCA TCTTCTTCCA GCAATCGTTC CCCTTCTTTG GCAATGAGTT CTTCAATTCG
    TTCGGAGGCT TTGGCTTTGG AGCTGCCCAG GAGCCCTGGT GGAAGGGACC CAATGTGTGC
    ACCGAAAAGG AGGAAGACGA GACTGTGGCC AGTGAAACGG AGGCAGAAGA AACCGTGGAC
    ACCTTTGGCC AGGAACGTGA TGGCGTTACC AACGTGGTGC GATCCCCACT CTTTGGCCAG
    TTCCAGTTCA GCGTTAACTC CTGCGCCGAG AAGCCCAACA AGCACGTCTG CACCAAGATC
    GTGAATCAAA ATGGCAAGAA GAAAACGCTG ACGCTGACCC GCCAGTGCTG CTATGGATAT
    GGACGCCCCA GAAACGCTGA CTTCACAACG CCCTGCGAGA AGATCGACAT CAAGGACGTG
    GAGGCCACGG CCAGTGACAT GGGTGCCAAG CAGTTCCTGG AGAGTGCACG CACCGCCGGT
    GAGCTGGCCG ATATGCTCGG AGCAGGCAGT GGCAAGAAGG TGACCCTCTT CGTGCCCAAC
    GACGCCGCCT TCATGGAGTA CCGTGGTCAT CATCAGCAGG AGAATAATGT GGAAGATGCC
    AAGGCCGAGG CTTCCAAGCC ATCCATCTAC AAACTGCACG CTGTTCTCGG CGAAGTTCAG
    CTGGAGGATG TGCCCAATGA GAAGCTTCTG CAGACGGAGC TGCCTGACCA GAAGATCCGC
    ATCAACAGCT ACCAGCTGCC TGCGGCTTTG GGCCTGGAAC CCTACCGCTT TGCCGCCAAC
    TGTGTGCCCA TCGAGAAGCA CGACAAGCTC TCGGAGCAGG CCCTTGTCCA CACTCTGGGC
    GGAGTCCTGA AGCCGCTGAC CAAGAACCTT ATGGATATCA TCAGGGAGCG TGCGGATATG
    TCCATTATGC GCACCGTTCT GGAGAAGACC AACCTGAGCG CCATGCTAGA GGATGACAAG
    CCAGTGACCA TCTTTGTGCC CACCGATGCC GCCTTCGACA AGTTGGAACC TCATCTGCGT
    CGAGCCCTCA AGGAAGGTCG CGGTTGTGCC TCCAACATCC TGAAGAACCA TTTGCTGGAC
    CTCACCTTCT GCTCGCTGGC CACTGTTCCG GGTGCCAAGA CGACGGCCTA CAATCTACTG
    GGAGAACCCC TGCTCCTCAA CCGAACCCAC CGCGCTGCGA ATCAGACTGG ACCGACTCCC
    ATCTACATCA ATAACTTGGC CAAAATCATT GATGCCGATA TCATGGGCAC CAATGGTGTG
    CTGCACGTAA TCGACACCAT CCTGCCCACC GAATCGGCTC TGCCCATGAC CTCGCTGATG
    TCGCAAAAGA ACCTGACCAT CTTCCAGCGA TTGCTGGAGG CCTCCGGCTA CGATGATCAG
    TTCGACGATC TGGACAACGT GACCATCTTT GCACCAACTG ACAAGGCTCT GCAGAATACG
    GAATGGGCTC GCATGCTCAA GGAACAGCCG GAGCTGCTGA ATCATAACTT GGATCTGCTG
    GAGTTCCTTA ACTACCATGT GGTCAAGCCC ATGATCAAGA CCTGTGACCT GTCGGAGAAG
    TCCCTGCCCA CGGTTGCTGG ATCTAGTGTG CGTCTGAACC TGTACTCCAC CCATGCTCTC
    TTCTCGGACG TGATGAACCG GGCAACGGTA AACTGTGCCC GTTTGGTGCA CTTCGATGAC
    GAGAGCTGCG GATCCGTTCT CCACCAGGTG GATCGTGCCT TGGCGCCTCC CAAGAATAAC
    ATGCTTAAGC TGCTGGAGGC TAATCCCAAC TACAGCAAGT TCCTGGAGCT GGTTCGTAAG
    GCTAATCTTA CACAGCTGCT GTCCAACGAT TCGAGGAGTC TCACCCTGCT GGTGCCAAAG
    AACGATATCT TCGAGGAGCT GAACGAGTCC GGTGAGGGTT CAAAGCCCGC CGATCCCATG
    GCCTTGGTCA AAACCCATAT CGTTGAGGAT GTTGTCTGCT GTGCCGGCAT TATCCCAACC
    AACTGGCCAT TTGTCCGCTC CATCGAGTCC ATCTCTGGCC AGCATCTGCG TATCACCCGC
    GATCGTCGTC CCAAGATCGA GAACGCCGGC GTCACCAAGT GCGATGTGGT GGCCACCAAT
    GGAATTCTCC ATGAGATCAA CGACATCATC GTGCCACGCC CACAGCGCCA GCAACAGCAG
    CGACCCCAGC TGATCCCGCC CGGAGCTGGC TATCAGCCAC AGGGCGATTT CGATGTCTTC
    TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788649.1
    CDS130..2775
    Translation

    Target ORF information:

    RefSeq Version NM_176472.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176472.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ACTTTATGCC ACATTTAACC CACCCATTGC CGGCGCATCG CAATCTGTTC
    GCGCCCCTGC CCTTCGCACC ACTCGCACCG CTCGAGCCCC AAGATCCACT GCATGCCCAG
    GCCTCGCAGC GGATGGCGAG CGTCTGGAAC GGACCCGCCC CGTTGGCGCC GCAACTCCAC
    TCATCTCACC TGTTGCCCCT CTTCAGCAGC GGATACGACA CCGAGTTCGT GCCACTGGAG
    CACCAGCAGC AGCCGCAGGG ACGCCAGGAG ACGGCGGCCA CCGTTCCCGC TCAAACCCCC
    AGCGGTGTGG AGCAGAAGCC GTTCAATGTG GACACCATCA CCACGGATGT GAATTCCCCA
    AATCCGGCCA TCTTCTTCCA GCAATCGTTC CCCTTCTTTG GCAATGAGTT CTTCAATTCG
    TTCGGAGGCT TTGGCTTTGG AGCTGCCCAG GAGCCCTGGT GGAAGGGACC CAATGTGTGC
    ACCGAAAAGG AGGAAGACGA GACTGTGGCC AGTGAAACGG AGGCAGAAGA AACCGTGGAC
    ACCTTTGGCC AGGAACGTGA TGGCGTTACC AACGTGGTGC GATCCCCACT CTTTGGCCAG
    TTCCAGTTCA GCGTTAACTC CTGCGCCGAG AAGCCCAACA AGCACGTCTG CACCAAGATC
    GTGAATCAAA ATGGCAAGAA GAAAACGCTG ACGCTGACCC GCCAGTGCTG CTATGGATAT
    GGACGCCCCA GAAACGCTGA CTTCACAACG CCCTGCGAGA AGATCGACAT CAAGGACGTG
    GAGGCCACGG CCAGTGACAT GGGTGCCAAG CAGTTCCTGG AGAGTGCACG CACCGCCGGT
    GAGCTGGCCG ATATGCTCGG AGCAGGCAGT GGCAAGAAGG TGACCCTCTT CGTGCCCAAC
    GACGCCGCCT TCATGGAGTA CCGTGGTCAT CATCAGCAGG AGAATAATGT GGAAGATGCC
    AAGGCCGAGG CTTCCAAGCC ATCCATCTAC AAACTGCACG CTGTTCTCGG CGAAGTTCAG
    CTGGAGGATG TGCCCAATGA GAAGCTTCTG CAGACGGAGC TGCCTGACCA GAAGATCCGC
    ATCAACAGCT ACCAGCTGCC TGCGGCTTTG GGCCTGGAAC CCTACCGCTT TGCCGCCAAC
    TGTGTGCCCA TCGAGAAGCA CGACAAGCTC TCGGAGCAGG CCCTTGTCCA CACTCTGGGC
    GGAGTCCTGA AGCCGCTGAC CAAGAACCTT ATGGATATCA TCAGGGAGCG TGCGGATATG
    TCCATTATGC GCACCGTTCT GGAGAAGACC AACCTGAGCG CCATGCTAGA GGATGACAAG
    CCAGTGACCA TCTTTGTGCC CACCGATGCC GCCTTCGACA AGTTGGAACC TCATCTGCGT
    CGAGCCCTCA AGGAAGGTCG CGGTTGTGCC TCCAACATCC TGAAGAACCA TTTGCTGGAC
    CTCACCTTCT GCTCGCTGGC CACTGTTCCG GGTGCCAAGA CGACGGCCTA CAATCTACTG
    GGAGAACCCC TGCTCCTCAA CCGAACCCAC CGCGCTGCGA ATCAGACTGG ACCGACTCCC
    ATCTACATCA ATAACTTGGC CAAAATCATT GATGCCGATA TCATGGGCAC CAATGGTGTG
    CTGCACGTAA TCGACACCAT CCTGCCCACC GAATCGGCTC TGCCCATGAC CTCGCTGATG
    TCGCAAAAGA ACCTGACCAT CTTCCAGCGA TTGCTGGAGG CCTCCGGCTA CGATGATCAG
    TTCGACGATC TGGACAACGT GACCATCTTT GCACCAACTG ACAAGGCTCT GCAGAATACG
    GAATGGGCTC GCATGCTCAA GGAACAGCCG GAGCTGCTGA ATCATAACTT GGATCTGCTG
    GAGTTCCTTA ACTACCATGT GGTCAAGCCC ATGATCAAGA CCTGTGACCT GTCGGAGAAG
    TCCCTGCCCA CGGTTGCTGG ATCTAGTGTG CGTCTGAACC TGTACTCCAC CCATGCTCTC
    TTCTCGGACG TGATGAACCG GGCAACGGTA AACTGTGCCC GTTTGGTGCA CTTCGATGAC
    GAGAGCTGCG GATCCGTTCT CCACCAGGTG GATCGTGCCT TGGCGCCTCC CAAGAATAAC
    ATGCTTAAGC TGCTGGAGGC TAATCCCAAC TACAGCAAGT TCCTGGAGCT GGTTCGTAAG
    GCTAATCTTA CACAGCTGCT GTCCAACGAT TCGAGGAGTC TCACCCTGCT GGTGCCAAAG
    AACGATATCT TCGAGGAGCT GAACGAGTCC GGTGAGGGTT CAAAGCCCGC CGATCCCATG
    GCCTTGGTCA AAACCCATAT CGTTGAGGAT GTTGTCTGCT GTGCCGGCAT TATCCCAACC
    AACTGGCCAT TTGTCCGCTC CATCGAGTCC ATCTCTGGCC AGCATCTGCG TATCACCCGC
    GATCGTCGTC CCAAGATCGA GAACGCCGGC GTCACCAAGT GCGATGTGGT GGCCACCAAT
    GGAATTCTCC ATGAGATCAA CGACATCATC GTGCCACGCC CACAGCGCCA GCAACAGCAG
    CGACCCCAGC TGATCCCGCC CGGAGCTGGC TATCAGCCAC AGGGCGATTT CGATGTCTTC
    TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04620
    Clone ID Related Accession (Same CDS sequence) NM_176473.1 , NM_176474.1
    Accession Version NM_176473.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ATTCAAGTTC TGAAGAGGTT GCCGAATTCG ATGACTCCTC GGATTCCACA
    CACGAGTCCT CAGATAACTC CAGCGGATAC GACACCGAGT TCGTGCCACT GGAGCACCAG
    CAGCAGCCGC AGGGACGCCA GGAGACGGCG GCCACCGTTC CCGCTCAAAC CCCCAGCGGT
    GTGGAGCAGA AGCCGTTCAA TGTGGACACC ATCACCACGG ATGTGAATTC CCCAAATCCG
    GCCATCTTCT TCCAGCAATC GTTCCCCTTC TTTGGCAATG AGTTCTTCAA TTCGTTCGGA
    GGCTTTGGCT TTGGAGCTGC CCAGGAGCCC TGGTGGAAGG GACCCAATGT GTGCACCGAA
    AAGGAGGAAG ACGAGACTGT GGCCAGTGAA ACGGAGGCAG AAGAAACCGT GGACACCTTT
    GGCCAGGAAC GTGATGGCGT TACCAACGTG GTGCGATCCC CACTCTTTGG CCAGTTCCAG
    TTCAGCGTTA ACTCCTGCGC CGAGAAGCCC AACAAGCACG TCTGCACCAA GATCGTGAAT
    CAAAATGGCA AGAAGAAAAC GCTGACGCTG ACCCGCCAGT GCTGCTATGG ATATGGACGC
    CCCAGAAACG CTGACTTCAC AACGCCCTGC GAGAAGATCG ACATCAAGGA CGTGGAGGCC
    ACGGCCAGTG ACATGGGTGC CAAGCAGTTC CTGGAGAGTG CACGCACCGC CGGTGAGCTG
    GCCGATATGC TCGGAGCAGG CAGTGGCAAG AAGGTGACCC TCTTCGTGCC CAACGACGCC
    GCCTTCATGG AGTACCGTGG TCATCATCAG CAGGAGAATA ATGTGGAAGA TGCCAAGGCC
    GAGGCTTCCA AGCCATCCAT CTACAAACTG CACGCTGTTC TCGGCGAAGT TCAGCTGGAG
    GATGTGCCCA ATGAGAAGCT TCTGCAGACG GAGCTGCCTG ACCAGAAGAT CCGCATCAAC
    AGCTACCAGC TGCCTGCGGC TTTGGGCCTG GAACCCTACC GCTTTGCCGC CAACTGTGTG
    CCCATCGAGA AGCACGACAA GCTCTCGGAG CAGGCCCTTG TCCACACTCT GGGCGGAGTC
    CTGAAGCCGC TGACCAAGAA CCTTATGGAT ATCATCAGGG AGCGTGCGGA TATGTCCATT
    ATGCGCACCG TTCTGGAGAA GACCAACCTG AGCGCCATGC TAGAGGATGA CAAGCCAGTG
    ACCATCTTTG TGCCCACCGA TGCCGCCTTC GACAAGTTGG AACCTCATCT GCGTCGAGCC
    CTCAAGGAAG GTCGCGGTTG TGCCTCCAAC ATCCTGAAGA ACCATTTGCT GGACCTCACC
    TTCTGCTCGC TGGCCACTGT TCCGGGTGCC AAGACGACGG CCTACAATCT ACTGGGAGAA
    CCCCTGCTCC TCAACCGAAC CCACCGCGCT GCGAATCAGA CTGGACCGAC TCCCATCTAC
    ATCAATAACT TGGCCAAAAT CATTGATGCC GATATCATGG GCACCAATGG TGTGCTGCAC
    GTAATCGACA CCATCCTGCC CACCGAATCG GCTCTGCCCA TGACCTCGCT GATGTCGCAA
    AAGAACCTGA CCATCTTCCA GCGATTGCTG GAGGCCTCCG GCTACGATGA TCAGTTCGAC
    GATCTGGACA ACGTGACCAT CTTTGCACCA ACTGACAAGG CTCTGCAGAA TACGGAATGG
    GCTCGCATGC TCAAGGAACA GCCGGAGCTG CTGAATCATA ACTTGGATCT GCTGGAGTTC
    CTTAACTACC ATGTGGTCAA GCCCATGATC AAGACCTGTG ACCTGTCGGA GAAGTCCCTG
    CCCACGGTTG CTGGATCTAG TGTGCGTCTG AACCTGTACT CCACCCATGC TCTCTTCTCG
    GACGTGATGA ACCGGGCAAC GGTAAACTGT GCCCGTTTGG TGCACTTCGA TGACGAGAGC
    TGCGGATCCG TTCTCCACCA GGTGGATCGT GCCTTGGCGC CTCCCAAGAA TAACATGCTT
    AAGCTGCTGG AGGCTAATCC CAACTACAGC AAGTTCCTGG AGCTGGTTCG TAAGGCTAAT
    CTTACACAGC TGCTGTCCAA CGATTCGAGG AGTCTCACCC TGCTGGTGCC AAAGAACGAT
    ATCTTCGAGG AGCTGAACGA GTCCGGTGAG GGTTCAAAGC CCGCCGATCC CATGGCCTTG
    GTCAAAACCC ATATCGTTGA GGATGTTGTC TGCTGTGCCG GCATTATCCC AACCAACTGG
    CCATTTGTCC GCTCCATCGA GTCCATCTCT GGCCAGCATC TGCGTATCAC CCGCGATCGT
    CGTCCCAAGA TCGAGAACGC CGGCGTCACC AAGTGCGATG TGGTGGCCAC CAATGGAATT
    CTCCATGAGA TCAACGACAT CATCGTGCCA CGCCCACAGC GCCAGCAACA GCAGCGACCC
    CAGCTGATCC CGCCCGGAGC TGGCTATCAG CCACAGGGCG ATTTCGATGT CTTCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788650.1
    CDS130..2649
    Translation

    Target ORF information:

    RefSeq Version NM_176473.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176473.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ATTCAAGTTC TGAAGAGGTT GCCGAATTCG ATGACTCCTC GGATTCCACA
    CACGAGTCCT CAGATAACTC CAGCGGATAC GACACCGAGT TCGTGCCACT GGAGCACCAG
    CAGCAGCCGC AGGGACGCCA GGAGACGGCG GCCACCGTTC CCGCTCAAAC CCCCAGCGGT
    GTGGAGCAGA AGCCGTTCAA TGTGGACACC ATCACCACGG ATGTGAATTC CCCAAATCCG
    GCCATCTTCT TCCAGCAATC GTTCCCCTTC TTTGGCAATG AGTTCTTCAA TTCGTTCGGA
    GGCTTTGGCT TTGGAGCTGC CCAGGAGCCC TGGTGGAAGG GACCCAATGT GTGCACCGAA
    AAGGAGGAAG ACGAGACTGT GGCCAGTGAA ACGGAGGCAG AAGAAACCGT GGACACCTTT
    GGCCAGGAAC GTGATGGCGT TACCAACGTG GTGCGATCCC CACTCTTTGG CCAGTTCCAG
    TTCAGCGTTA ACTCCTGCGC CGAGAAGCCC AACAAGCACG TCTGCACCAA GATCGTGAAT
    CAAAATGGCA AGAAGAAAAC GCTGACGCTG ACCCGCCAGT GCTGCTATGG ATATGGACGC
    CCCAGAAACG CTGACTTCAC AACGCCCTGC GAGAAGATCG ACATCAAGGA CGTGGAGGCC
    ACGGCCAGTG ACATGGGTGC CAAGCAGTTC CTGGAGAGTG CACGCACCGC CGGTGAGCTG
    GCCGATATGC TCGGAGCAGG CAGTGGCAAG AAGGTGACCC TCTTCGTGCC CAACGACGCC
    GCCTTCATGG AGTACCGTGG TCATCATCAG CAGGAGAATA ATGTGGAAGA TGCCAAGGCC
    GAGGCTTCCA AGCCATCCAT CTACAAACTG CACGCTGTTC TCGGCGAAGT TCAGCTGGAG
    GATGTGCCCA ATGAGAAGCT TCTGCAGACG GAGCTGCCTG ACCAGAAGAT CCGCATCAAC
    AGCTACCAGC TGCCTGCGGC TTTGGGCCTG GAACCCTACC GCTTTGCCGC CAACTGTGTG
    CCCATCGAGA AGCACGACAA GCTCTCGGAG CAGGCCCTTG TCCACACTCT GGGCGGAGTC
    CTGAAGCCGC TGACCAAGAA CCTTATGGAT ATCATCAGGG AGCGTGCGGA TATGTCCATT
    ATGCGCACCG TTCTGGAGAA GACCAACCTG AGCGCCATGC TAGAGGATGA CAAGCCAGTG
    ACCATCTTTG TGCCCACCGA TGCCGCCTTC GACAAGTTGG AACCTCATCT GCGTCGAGCC
    CTCAAGGAAG GTCGCGGTTG TGCCTCCAAC ATCCTGAAGA ACCATTTGCT GGACCTCACC
    TTCTGCTCGC TGGCCACTGT TCCGGGTGCC AAGACGACGG CCTACAATCT ACTGGGAGAA
    CCCCTGCTCC TCAACCGAAC CCACCGCGCT GCGAATCAGA CTGGACCGAC TCCCATCTAC
    ATCAATAACT TGGCCAAAAT CATTGATGCC GATATCATGG GCACCAATGG TGTGCTGCAC
    GTAATCGACA CCATCCTGCC CACCGAATCG GCTCTGCCCA TGACCTCGCT GATGTCGCAA
    AAGAACCTGA CCATCTTCCA GCGATTGCTG GAGGCCTCCG GCTACGATGA TCAGTTCGAC
    GATCTGGACA ACGTGACCAT CTTTGCACCA ACTGACAAGG CTCTGCAGAA TACGGAATGG
    GCTCGCATGC TCAAGGAACA GCCGGAGCTG CTGAATCATA ACTTGGATCT GCTGGAGTTC
    CTTAACTACC ATGTGGTCAA GCCCATGATC AAGACCTGTG ACCTGTCGGA GAAGTCCCTG
    CCCACGGTTG CTGGATCTAG TGTGCGTCTG AACCTGTACT CCACCCATGC TCTCTTCTCG
    GACGTGATGA ACCGGGCAAC GGTAAACTGT GCCCGTTTGG TGCACTTCGA TGACGAGAGC
    TGCGGATCCG TTCTCCACCA GGTGGATCGT GCCTTGGCGC CTCCCAAGAA TAACATGCTT
    AAGCTGCTGG AGGCTAATCC CAACTACAGC AAGTTCCTGG AGCTGGTTCG TAAGGCTAAT
    CTTACACAGC TGCTGTCCAA CGATTCGAGG AGTCTCACCC TGCTGGTGCC AAAGAACGAT
    ATCTTCGAGG AGCTGAACGA GTCCGGTGAG GGTTCAAAGC CCGCCGATCC CATGGCCTTG
    GTCAAAACCC ATATCGTTGA GGATGTTGTC TGCTGTGCCG GCATTATCCC AACCAACTGG
    CCATTTGTCC GCTCCATCGA GTCCATCTCT GGCCAGCATC TGCGTATCAC CCGCGATCGT
    CGTCCCAAGA TCGAGAACGC CGGCGTCACC AAGTGCGATG TGGTGGCCAC CAATGGAATT
    CTCCATGAGA TCAACGACAT CATCGTGCCA CGCCCACAGC GCCAGCAACA GCAGCGACCC
    CAGCTGATCC CGCCCGGAGC TGGCTATCAG CCACAGGGCG ATTTCGATGT CTTCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04620
    Clone ID Related Accession (Same CDS sequence) NM_176473.1 , NM_176474.1
    Accession Version NM_176474.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform O
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ATTCAAGTTC TGAAGAGGTT GCCGAATTCG ATGACTCCTC GGATTCCACA
    CACGAGTCCT CAGATAACTC CAGCGGATAC GACACCGAGT TCGTGCCACT GGAGCACCAG
    CAGCAGCCGC AGGGACGCCA GGAGACGGCG GCCACCGTTC CCGCTCAAAC CCCCAGCGGT
    GTGGAGCAGA AGCCGTTCAA TGTGGACACC ATCACCACGG ATGTGAATTC CCCAAATCCG
    GCCATCTTCT TCCAGCAATC GTTCCCCTTC TTTGGCAATG AGTTCTTCAA TTCGTTCGGA
    GGCTTTGGCT TTGGAGCTGC CCAGGAGCCC TGGTGGAAGG GACCCAATGT GTGCACCGAA
    AAGGAGGAAG ACGAGACTGT GGCCAGTGAA ACGGAGGCAG AAGAAACCGT GGACACCTTT
    GGCCAGGAAC GTGATGGCGT TACCAACGTG GTGCGATCCC CACTCTTTGG CCAGTTCCAG
    TTCAGCGTTA ACTCCTGCGC CGAGAAGCCC AACAAGCACG TCTGCACCAA GATCGTGAAT
    CAAAATGGCA AGAAGAAAAC GCTGACGCTG ACCCGCCAGT GCTGCTATGG ATATGGACGC
    CCCAGAAACG CTGACTTCAC AACGCCCTGC GAGAAGATCG ACATCAAGGA CGTGGAGGCC
    ACGGCCAGTG ACATGGGTGC CAAGCAGTTC CTGGAGAGTG CACGCACCGC CGGTGAGCTG
    GCCGATATGC TCGGAGCAGG CAGTGGCAAG AAGGTGACCC TCTTCGTGCC CAACGACGCC
    GCCTTCATGG AGTACCGTGG TCATCATCAG CAGGAGAATA ATGTGGAAGA TGCCAAGGCC
    GAGGCTTCCA AGCCATCCAT CTACAAACTG CACGCTGTTC TCGGCGAAGT TCAGCTGGAG
    GATGTGCCCA ATGAGAAGCT TCTGCAGACG GAGCTGCCTG ACCAGAAGAT CCGCATCAAC
    AGCTACCAGC TGCCTGCGGC TTTGGGCCTG GAACCCTACC GCTTTGCCGC CAACTGTGTG
    CCCATCGAGA AGCACGACAA GCTCTCGGAG CAGGCCCTTG TCCACACTCT GGGCGGAGTC
    CTGAAGCCGC TGACCAAGAA CCTTATGGAT ATCATCAGGG AGCGTGCGGA TATGTCCATT
    ATGCGCACCG TTCTGGAGAA GACCAACCTG AGCGCCATGC TAGAGGATGA CAAGCCAGTG
    ACCATCTTTG TGCCCACCGA TGCCGCCTTC GACAAGTTGG AACCTCATCT GCGTCGAGCC
    CTCAAGGAAG GTCGCGGTTG TGCCTCCAAC ATCCTGAAGA ACCATTTGCT GGACCTCACC
    TTCTGCTCGC TGGCCACTGT TCCGGGTGCC AAGACGACGG CCTACAATCT ACTGGGAGAA
    CCCCTGCTCC TCAACCGAAC CCACCGCGCT GCGAATCAGA CTGGACCGAC TCCCATCTAC
    ATCAATAACT TGGCCAAAAT CATTGATGCC GATATCATGG GCACCAATGG TGTGCTGCAC
    GTAATCGACA CCATCCTGCC CACCGAATCG GCTCTGCCCA TGACCTCGCT GATGTCGCAA
    AAGAACCTGA CCATCTTCCA GCGATTGCTG GAGGCCTCCG GCTACGATGA TCAGTTCGAC
    GATCTGGACA ACGTGACCAT CTTTGCACCA ACTGACAAGG CTCTGCAGAA TACGGAATGG
    GCTCGCATGC TCAAGGAACA GCCGGAGCTG CTGAATCATA ACTTGGATCT GCTGGAGTTC
    CTTAACTACC ATGTGGTCAA GCCCATGATC AAGACCTGTG ACCTGTCGGA GAAGTCCCTG
    CCCACGGTTG CTGGATCTAG TGTGCGTCTG AACCTGTACT CCACCCATGC TCTCTTCTCG
    GACGTGATGA ACCGGGCAAC GGTAAACTGT GCCCGTTTGG TGCACTTCGA TGACGAGAGC
    TGCGGATCCG TTCTCCACCA GGTGGATCGT GCCTTGGCGC CTCCCAAGAA TAACATGCTT
    AAGCTGCTGG AGGCTAATCC CAACTACAGC AAGTTCCTGG AGCTGGTTCG TAAGGCTAAT
    CTTACACAGC TGCTGTCCAA CGATTCGAGG AGTCTCACCC TGCTGGTGCC AAAGAACGAT
    ATCTTCGAGG AGCTGAACGA GTCCGGTGAG GGTTCAAAGC CCGCCGATCC CATGGCCTTG
    GTCAAAACCC ATATCGTTGA GGATGTTGTC TGCTGTGCCG GCATTATCCC AACCAACTGG
    CCATTTGTCC GCTCCATCGA GTCCATCTCT GGCCAGCATC TGCGTATCAC CCGCGATCGT
    CGTCCCAAGA TCGAGAACGC CGGCGTCACC AAGTGCGATG TGGTGGCCAC CAATGGAATT
    CTCCATGAGA TCAACGACAT CATCGTGCCA CGCCCACAGC GCCAGCAACA GCAGCGACCC
    CAGCTGATCC CGCCCGGAGC TGGCTATCAG CCACAGGGCG ATTTCGATGT CTTCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788651.1
    CDS108..2627
    Translation

    Target ORF information:

    RefSeq Version NM_176474.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant O, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176474.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ATTCAAGTTC TGAAGAGGTT GCCGAATTCG ATGACTCCTC GGATTCCACA
    CACGAGTCCT CAGATAACTC CAGCGGATAC GACACCGAGT TCGTGCCACT GGAGCACCAG
    CAGCAGCCGC AGGGACGCCA GGAGACGGCG GCCACCGTTC CCGCTCAAAC CCCCAGCGGT
    GTGGAGCAGA AGCCGTTCAA TGTGGACACC ATCACCACGG ATGTGAATTC CCCAAATCCG
    GCCATCTTCT TCCAGCAATC GTTCCCCTTC TTTGGCAATG AGTTCTTCAA TTCGTTCGGA
    GGCTTTGGCT TTGGAGCTGC CCAGGAGCCC TGGTGGAAGG GACCCAATGT GTGCACCGAA
    AAGGAGGAAG ACGAGACTGT GGCCAGTGAA ACGGAGGCAG AAGAAACCGT GGACACCTTT
    GGCCAGGAAC GTGATGGCGT TACCAACGTG GTGCGATCCC CACTCTTTGG CCAGTTCCAG
    TTCAGCGTTA ACTCCTGCGC CGAGAAGCCC AACAAGCACG TCTGCACCAA GATCGTGAAT
    CAAAATGGCA AGAAGAAAAC GCTGACGCTG ACCCGCCAGT GCTGCTATGG ATATGGACGC
    CCCAGAAACG CTGACTTCAC AACGCCCTGC GAGAAGATCG ACATCAAGGA CGTGGAGGCC
    ACGGCCAGTG ACATGGGTGC CAAGCAGTTC CTGGAGAGTG CACGCACCGC CGGTGAGCTG
    GCCGATATGC TCGGAGCAGG CAGTGGCAAG AAGGTGACCC TCTTCGTGCC CAACGACGCC
    GCCTTCATGG AGTACCGTGG TCATCATCAG CAGGAGAATA ATGTGGAAGA TGCCAAGGCC
    GAGGCTTCCA AGCCATCCAT CTACAAACTG CACGCTGTTC TCGGCGAAGT TCAGCTGGAG
    GATGTGCCCA ATGAGAAGCT TCTGCAGACG GAGCTGCCTG ACCAGAAGAT CCGCATCAAC
    AGCTACCAGC TGCCTGCGGC TTTGGGCCTG GAACCCTACC GCTTTGCCGC CAACTGTGTG
    CCCATCGAGA AGCACGACAA GCTCTCGGAG CAGGCCCTTG TCCACACTCT GGGCGGAGTC
    CTGAAGCCGC TGACCAAGAA CCTTATGGAT ATCATCAGGG AGCGTGCGGA TATGTCCATT
    ATGCGCACCG TTCTGGAGAA GACCAACCTG AGCGCCATGC TAGAGGATGA CAAGCCAGTG
    ACCATCTTTG TGCCCACCGA TGCCGCCTTC GACAAGTTGG AACCTCATCT GCGTCGAGCC
    CTCAAGGAAG GTCGCGGTTG TGCCTCCAAC ATCCTGAAGA ACCATTTGCT GGACCTCACC
    TTCTGCTCGC TGGCCACTGT TCCGGGTGCC AAGACGACGG CCTACAATCT ACTGGGAGAA
    CCCCTGCTCC TCAACCGAAC CCACCGCGCT GCGAATCAGA CTGGACCGAC TCCCATCTAC
    ATCAATAACT TGGCCAAAAT CATTGATGCC GATATCATGG GCACCAATGG TGTGCTGCAC
    GTAATCGACA CCATCCTGCC CACCGAATCG GCTCTGCCCA TGACCTCGCT GATGTCGCAA
    AAGAACCTGA CCATCTTCCA GCGATTGCTG GAGGCCTCCG GCTACGATGA TCAGTTCGAC
    GATCTGGACA ACGTGACCAT CTTTGCACCA ACTGACAAGG CTCTGCAGAA TACGGAATGG
    GCTCGCATGC TCAAGGAACA GCCGGAGCTG CTGAATCATA ACTTGGATCT GCTGGAGTTC
    CTTAACTACC ATGTGGTCAA GCCCATGATC AAGACCTGTG ACCTGTCGGA GAAGTCCCTG
    CCCACGGTTG CTGGATCTAG TGTGCGTCTG AACCTGTACT CCACCCATGC TCTCTTCTCG
    GACGTGATGA ACCGGGCAAC GGTAAACTGT GCCCGTTTGG TGCACTTCGA TGACGAGAGC
    TGCGGATCCG TTCTCCACCA GGTGGATCGT GCCTTGGCGC CTCCCAAGAA TAACATGCTT
    AAGCTGCTGG AGGCTAATCC CAACTACAGC AAGTTCCTGG AGCTGGTTCG TAAGGCTAAT
    CTTACACAGC TGCTGTCCAA CGATTCGAGG AGTCTCACCC TGCTGGTGCC AAAGAACGAT
    ATCTTCGAGG AGCTGAACGA GTCCGGTGAG GGTTCAAAGC CCGCCGATCC CATGGCCTTG
    GTCAAAACCC ATATCGTTGA GGATGTTGTC TGCTGTGCCG GCATTATCCC AACCAACTGG
    CCATTTGTCC GCTCCATCGA GTCCATCTCT GGCCAGCATC TGCGTATCAC CCGCGATCGT
    CGTCCCAAGA TCGAGAACGC CGGCGTCACC AAGTGCGATG TGGTGGCCAC CAATGGAATT
    CTCCATGAGA TCAACGACAT CATCGTGCCA CGCCCACAGC GCCAGCAACA GCAGCGACCC
    CAGCTGATCC CGCCCGGAGC TGGCTATCAG CCACAGGGCG ATTTCGATGT CTTCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04621
    Clone ID Related Accession (Same CDS sequence) NM_176475.1 , NM_176476.1 , NM_176477.1
    Accession Version NM_176475.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ATTCAAGTTC TGAAGAGGTT GCCGAATTCG ATGACTCCTC GGATTCCACA
    CACGAGTCCT CAGATAACTC CAGCAGCGGA TACGACACCG AGTTCGTGCC ACTGGAGCAC
    CAGCAGCAGC CGCAGGGACG CCAGGAGACG GCGGCCACCG TTCCCGCTCA AACCCCCAGC
    GGTGTGGAGC AGAAGCCGTT CAATGTGGAC ACCATCACCA CGGATGTGAA TTCCCCAAAT
    CCGGCCATCT TCTTCCAGCA ATCGTTCCCC TTCTTTGGCA ATGAGTTCTT CAATTCGTTC
    GGAGGCTTTG GCTTTGGAGC TGCCCAGGAG CCCTGGTGGA AGGGACCCAA TGTGTGCACC
    GAAAAGGAGG AAGACGAGAC TGTGGCCAGT GAAACGGAGG CAGAAGAAAC CGTGGACACC
    TTTGGCCAGG AACGTGATGG CGTTACCAAC GTGGTGCGAT CCCCACTCTT TGGCCAGTTC
    CAGTTCAGCG TTAACTCCTG CGCCGAGAAG CCCAACAAGC ACGTCTGCAC CAAGATCGTG
    AATCAAAATG GCAAGAAGAA AACGCTGACG CTGACCCGCC AGTGCTGCTA TGGATATGGA
    CGCCCCAGAA ACGCTGACTT CACAACGCCC TGCGAGAAGA TCGACATCAA GGACGTGGAG
    GCCACGGCCA GTGACATGGG TGCCAAGCAG TTCCTGGAGA GTGCACGCAC CGCCGGTGAG
    CTGGCCGATA TGCTCGGAGC AGGCAGTGGC AAGAAGGTGA CCCTCTTCGT GCCCAACGAC
    GCCGCCTTCA TGGAGTACCG TGGTCATCAT CAGCAGGAGA ATAATGTGGA AGATGCCAAG
    GCCGAGGCTT CCAAGCCATC CATCTACAAA CTGCACGCTG TTCTCGGCGA AGTTCAGCTG
    GAGGATGTGC CCAATGAGAA GCTTCTGCAG ACGGAGCTGC CTGACCAGAA GATCCGCATC
    AACAGCTACC AGCTGCCTGC GGCTTTGGGC CTGGAACCCT ACCGCTTTGC CGCCAACTGT
    GTGCCCATCG AGAAGCACGA CAAGCTCTCG GAGCAGGCCC TTGTCCACAC TCTGGGCGGA
    GTCCTGAAGC CGCTGACCAA GAACCTTATG GATATCATCA GGGAGCGTGC GGATATGTCC
    ATTATGCGCA CCGTTCTGGA GAAGACCAAC CTGAGCGCCA TGCTAGAGGA TGACAAGCCA
    GTGACCATCT TTGTGCCCAC CGATGCCGCC TTCGACAAGT TGGAACCTCA TCTGCGTCGA
    GCCCTCAAGG AAGGTCGCGG TTGTGCCTCC AACATCCTGA AGAACCATTT GCTGGACCTC
    ACCTTCTGCT CGCTGGCCAC TGTTCCGGGT GCCAAGACGA CGGCCTACAA TCTACTGGGA
    GAACCCCTGC TCCTCAACCG AACCCACCGC GCTGCGAATC AGACTGGACC GACTCCCATC
    TACATCAATA ACTTGGCCAA AATCATTGAT GCCGATATCA TGGGCACCAA TGGTGTGCTG
    CACGTAATCG ACACCATCCT GCCCACCGAA TCGGCTCTGC CCATGACCTC GCTGATGTCG
    CAAAAGAACC TGACCATCTT CCAGCGATTG CTGGAGGCCT CCGGCTACGA TGATCAGTTC
    GACGATCTGG ACAACGTGAC CATCTTTGCA CCAACTGACA AGGCTCTGCA GAATACGGAA
    TGGGCTCGCA TGCTCAAGGA ACAGCCGGAG CTGCTGAATC ATAACTTGGA TCTGCTGGAG
    TTCCTTAACT ACCATGTGGT CAAGCCCATG ATCAAGACCT GTGACCTGTC GGAGAAGTCC
    CTGCCCACGG TTGCTGGATC TAGTGTGCGT CTGAACCTGT ACTCCACCCA TGCTCTCTTC
    TCGGACGTGA TGAACCGGGC AACGGTAAAC TGTGCCCGTT TGGTGCACTT CGATGACGAG
    AGCTGCGGAT CCGTTCTCCA CCAGGTGGAT CGTGCCTTGG CGCCTCCCAA GAATAACATG
    CTTAAGCTGC TGGAGGCTAA TCCCAACTAC AGCAAGTTCC TGGAGCTGGT TCGTAAGGCT
    AATCTTACAC AGCTGCTGTC CAACGATTCG AGGAGTCTCA CCCTGCTGGT GCCAAAGAAC
    GATATCTTCG AGGAGCTGAA CGAGTCCGGT GAGGGTTCAA AGCCCGCCGA TCCCATGGCC
    TTGGTCAAAA CCCATATCGT TGAGGATGTT GTCTGCTGTG CCGGCATTAT CCCAACCAAC
    TGGCCATTTG TCCGCTCCAT CGAGTCCATC TCTGGCCAGC ATCTGCGTAT CACCCGCGAT
    CGTCGTCCCA AGATCGAGAA CGCCGGCGTC ACCAAGTGCG ATGTGGTGGC CACCAATGGA
    ATTCTCCATG AGATCAACGA CATCATCGTG CCACGCCCAC AGCGCCAGCA ACAGCAGCGA
    CCCCAGCTGA TCCCGCCCGG AGCTGGCTAT CAGCCACAGG GCGATTTCGA TGTCTTCTTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788652.1
    CDS504..3026
    Translation

    Target ORF information:

    RefSeq Version NM_176475.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176475.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ATTCAAGTTC TGAAGAGGTT GCCGAATTCG ATGACTCCTC GGATTCCACA
    CACGAGTCCT CAGATAACTC CAGCAGCGGA TACGACACCG AGTTCGTGCC ACTGGAGCAC
    CAGCAGCAGC CGCAGGGACG CCAGGAGACG GCGGCCACCG TTCCCGCTCA AACCCCCAGC
    GGTGTGGAGC AGAAGCCGTT CAATGTGGAC ACCATCACCA CGGATGTGAA TTCCCCAAAT
    CCGGCCATCT TCTTCCAGCA ATCGTTCCCC TTCTTTGGCA ATGAGTTCTT CAATTCGTTC
    GGAGGCTTTG GCTTTGGAGC TGCCCAGGAG CCCTGGTGGA AGGGACCCAA TGTGTGCACC
    GAAAAGGAGG AAGACGAGAC TGTGGCCAGT GAAACGGAGG CAGAAGAAAC CGTGGACACC
    TTTGGCCAGG AACGTGATGG CGTTACCAAC GTGGTGCGAT CCCCACTCTT TGGCCAGTTC
    CAGTTCAGCG TTAACTCCTG CGCCGAGAAG CCCAACAAGC ACGTCTGCAC CAAGATCGTG
    AATCAAAATG GCAAGAAGAA AACGCTGACG CTGACCCGCC AGTGCTGCTA TGGATATGGA
    CGCCCCAGAA ACGCTGACTT CACAACGCCC TGCGAGAAGA TCGACATCAA GGACGTGGAG
    GCCACGGCCA GTGACATGGG TGCCAAGCAG TTCCTGGAGA GTGCACGCAC CGCCGGTGAG
    CTGGCCGATA TGCTCGGAGC AGGCAGTGGC AAGAAGGTGA CCCTCTTCGT GCCCAACGAC
    GCCGCCTTCA TGGAGTACCG TGGTCATCAT CAGCAGGAGA ATAATGTGGA AGATGCCAAG
    GCCGAGGCTT CCAAGCCATC CATCTACAAA CTGCACGCTG TTCTCGGCGA AGTTCAGCTG
    GAGGATGTGC CCAATGAGAA GCTTCTGCAG ACGGAGCTGC CTGACCAGAA GATCCGCATC
    AACAGCTACC AGCTGCCTGC GGCTTTGGGC CTGGAACCCT ACCGCTTTGC CGCCAACTGT
    GTGCCCATCG AGAAGCACGA CAAGCTCTCG GAGCAGGCCC TTGTCCACAC TCTGGGCGGA
    GTCCTGAAGC CGCTGACCAA GAACCTTATG GATATCATCA GGGAGCGTGC GGATATGTCC
    ATTATGCGCA CCGTTCTGGA GAAGACCAAC CTGAGCGCCA TGCTAGAGGA TGACAAGCCA
    GTGACCATCT TTGTGCCCAC CGATGCCGCC TTCGACAAGT TGGAACCTCA TCTGCGTCGA
    GCCCTCAAGG AAGGTCGCGG TTGTGCCTCC AACATCCTGA AGAACCATTT GCTGGACCTC
    ACCTTCTGCT CGCTGGCCAC TGTTCCGGGT GCCAAGACGA CGGCCTACAA TCTACTGGGA
    GAACCCCTGC TCCTCAACCG AACCCACCGC GCTGCGAATC AGACTGGACC GACTCCCATC
    TACATCAATA ACTTGGCCAA AATCATTGAT GCCGATATCA TGGGCACCAA TGGTGTGCTG
    CACGTAATCG ACACCATCCT GCCCACCGAA TCGGCTCTGC CCATGACCTC GCTGATGTCG
    CAAAAGAACC TGACCATCTT CCAGCGATTG CTGGAGGCCT CCGGCTACGA TGATCAGTTC
    GACGATCTGG ACAACGTGAC CATCTTTGCA CCAACTGACA AGGCTCTGCA GAATACGGAA
    TGGGCTCGCA TGCTCAAGGA ACAGCCGGAG CTGCTGAATC ATAACTTGGA TCTGCTGGAG
    TTCCTTAACT ACCATGTGGT CAAGCCCATG ATCAAGACCT GTGACCTGTC GGAGAAGTCC
    CTGCCCACGG TTGCTGGATC TAGTGTGCGT CTGAACCTGT ACTCCACCCA TGCTCTCTTC
    TCGGACGTGA TGAACCGGGC AACGGTAAAC TGTGCCCGTT TGGTGCACTT CGATGACGAG
    AGCTGCGGAT CCGTTCTCCA CCAGGTGGAT CGTGCCTTGG CGCCTCCCAA GAATAACATG
    CTTAAGCTGC TGGAGGCTAA TCCCAACTAC AGCAAGTTCC TGGAGCTGGT TCGTAAGGCT
    AATCTTACAC AGCTGCTGTC CAACGATTCG AGGAGTCTCA CCCTGCTGGT GCCAAAGAAC
    GATATCTTCG AGGAGCTGAA CGAGTCCGGT GAGGGTTCAA AGCCCGCCGA TCCCATGGCC
    TTGGTCAAAA CCCATATCGT TGAGGATGTT GTCTGCTGTG CCGGCATTAT CCCAACCAAC
    TGGCCATTTG TCCGCTCCAT CGAGTCCATC TCTGGCCAGC ATCTGCGTAT CACCCGCGAT
    CGTCGTCCCA AGATCGAGAA CGCCGGCGTC ACCAAGTGCG ATGTGGTGGC CACCAATGGA
    ATTCTCCATG AGATCAACGA CATCATCGTG CCACGCCCAC AGCGCCAGCA ACAGCAGCGA
    CCCCAGCTGA TCCCGCCCGG AGCTGGCTAT CAGCCACAGG GCGATTTCGA TGTCTTCTTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04621
    Clone ID Related Accession (Same CDS sequence) NM_176475.1 , NM_176476.1 , NM_176477.1
    Accession Version NM_176476.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ATTCAAGTTC TGAAGAGGTT GCCGAATTCG ATGACTCCTC GGATTCCACA
    CACGAGTCCT CAGATAACTC CAGCAGCGGA TACGACACCG AGTTCGTGCC ACTGGAGCAC
    CAGCAGCAGC CGCAGGGACG CCAGGAGACG GCGGCCACCG TTCCCGCTCA AACCCCCAGC
    GGTGTGGAGC AGAAGCCGTT CAATGTGGAC ACCATCACCA CGGATGTGAA TTCCCCAAAT
    CCGGCCATCT TCTTCCAGCA ATCGTTCCCC TTCTTTGGCA ATGAGTTCTT CAATTCGTTC
    GGAGGCTTTG GCTTTGGAGC TGCCCAGGAG CCCTGGTGGA AGGGACCCAA TGTGTGCACC
    GAAAAGGAGG AAGACGAGAC TGTGGCCAGT GAAACGGAGG CAGAAGAAAC CGTGGACACC
    TTTGGCCAGG AACGTGATGG CGTTACCAAC GTGGTGCGAT CCCCACTCTT TGGCCAGTTC
    CAGTTCAGCG TTAACTCCTG CGCCGAGAAG CCCAACAAGC ACGTCTGCAC CAAGATCGTG
    AATCAAAATG GCAAGAAGAA AACGCTGACG CTGACCCGCC AGTGCTGCTA TGGATATGGA
    CGCCCCAGAA ACGCTGACTT CACAACGCCC TGCGAGAAGA TCGACATCAA GGACGTGGAG
    GCCACGGCCA GTGACATGGG TGCCAAGCAG TTCCTGGAGA GTGCACGCAC CGCCGGTGAG
    CTGGCCGATA TGCTCGGAGC AGGCAGTGGC AAGAAGGTGA CCCTCTTCGT GCCCAACGAC
    GCCGCCTTCA TGGAGTACCG TGGTCATCAT CAGCAGGAGA ATAATGTGGA AGATGCCAAG
    GCCGAGGCTT CCAAGCCATC CATCTACAAA CTGCACGCTG TTCTCGGCGA AGTTCAGCTG
    GAGGATGTGC CCAATGAGAA GCTTCTGCAG ACGGAGCTGC CTGACCAGAA GATCCGCATC
    AACAGCTACC AGCTGCCTGC GGCTTTGGGC CTGGAACCCT ACCGCTTTGC CGCCAACTGT
    GTGCCCATCG AGAAGCACGA CAAGCTCTCG GAGCAGGCCC TTGTCCACAC TCTGGGCGGA
    GTCCTGAAGC CGCTGACCAA GAACCTTATG GATATCATCA GGGAGCGTGC GGATATGTCC
    ATTATGCGCA CCGTTCTGGA GAAGACCAAC CTGAGCGCCA TGCTAGAGGA TGACAAGCCA
    GTGACCATCT TTGTGCCCAC CGATGCCGCC TTCGACAAGT TGGAACCTCA TCTGCGTCGA
    GCCCTCAAGG AAGGTCGCGG TTGTGCCTCC AACATCCTGA AGAACCATTT GCTGGACCTC
    ACCTTCTGCT CGCTGGCCAC TGTTCCGGGT GCCAAGACGA CGGCCTACAA TCTACTGGGA
    GAACCCCTGC TCCTCAACCG AACCCACCGC GCTGCGAATC AGACTGGACC GACTCCCATC
    TACATCAATA ACTTGGCCAA AATCATTGAT GCCGATATCA TGGGCACCAA TGGTGTGCTG
    CACGTAATCG ACACCATCCT GCCCACCGAA TCGGCTCTGC CCATGACCTC GCTGATGTCG
    CAAAAGAACC TGACCATCTT CCAGCGATTG CTGGAGGCCT CCGGCTACGA TGATCAGTTC
    GACGATCTGG ACAACGTGAC CATCTTTGCA CCAACTGACA AGGCTCTGCA GAATACGGAA
    TGGGCTCGCA TGCTCAAGGA ACAGCCGGAG CTGCTGAATC ATAACTTGGA TCTGCTGGAG
    TTCCTTAACT ACCATGTGGT CAAGCCCATG ATCAAGACCT GTGACCTGTC GGAGAAGTCC
    CTGCCCACGG TTGCTGGATC TAGTGTGCGT CTGAACCTGT ACTCCACCCA TGCTCTCTTC
    TCGGACGTGA TGAACCGGGC AACGGTAAAC TGTGCCCGTT TGGTGCACTT CGATGACGAG
    AGCTGCGGAT CCGTTCTCCA CCAGGTGGAT CGTGCCTTGG CGCCTCCCAA GAATAACATG
    CTTAAGCTGC TGGAGGCTAA TCCCAACTAC AGCAAGTTCC TGGAGCTGGT TCGTAAGGCT
    AATCTTACAC AGCTGCTGTC CAACGATTCG AGGAGTCTCA CCCTGCTGGT GCCAAAGAAC
    GATATCTTCG AGGAGCTGAA CGAGTCCGGT GAGGGTTCAA AGCCCGCCGA TCCCATGGCC
    TTGGTCAAAA CCCATATCGT TGAGGATGTT GTCTGCTGTG CCGGCATTAT CCCAACCAAC
    TGGCCATTTG TCCGCTCCAT CGAGTCCATC TCTGGCCAGC ATCTGCGTAT CACCCGCGAT
    CGTCGTCCCA AGATCGAGAA CGCCGGCGTC ACCAAGTGCG ATGTGGTGGC CACCAATGGA
    ATTCTCCATG AGATCAACGA CATCATCGTG CCACGCCCAC AGCGCCAGCA ACAGCAGCGA
    CCCCAGCTGA TCCCGCCCGG AGCTGGCTAT CAGCCACAGG GCGATTTCGA TGTCTTCTTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788653.1
    CDS108..2630
    Translation

    Target ORF information:

    RefSeq Version NM_176476.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176476.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ATTCAAGTTC TGAAGAGGTT GCCGAATTCG ATGACTCCTC GGATTCCACA
    CACGAGTCCT CAGATAACTC CAGCAGCGGA TACGACACCG AGTTCGTGCC ACTGGAGCAC
    CAGCAGCAGC CGCAGGGACG CCAGGAGACG GCGGCCACCG TTCCCGCTCA AACCCCCAGC
    GGTGTGGAGC AGAAGCCGTT CAATGTGGAC ACCATCACCA CGGATGTGAA TTCCCCAAAT
    CCGGCCATCT TCTTCCAGCA ATCGTTCCCC TTCTTTGGCA ATGAGTTCTT CAATTCGTTC
    GGAGGCTTTG GCTTTGGAGC TGCCCAGGAG CCCTGGTGGA AGGGACCCAA TGTGTGCACC
    GAAAAGGAGG AAGACGAGAC TGTGGCCAGT GAAACGGAGG CAGAAGAAAC CGTGGACACC
    TTTGGCCAGG AACGTGATGG CGTTACCAAC GTGGTGCGAT CCCCACTCTT TGGCCAGTTC
    CAGTTCAGCG TTAACTCCTG CGCCGAGAAG CCCAACAAGC ACGTCTGCAC CAAGATCGTG
    AATCAAAATG GCAAGAAGAA AACGCTGACG CTGACCCGCC AGTGCTGCTA TGGATATGGA
    CGCCCCAGAA ACGCTGACTT CACAACGCCC TGCGAGAAGA TCGACATCAA GGACGTGGAG
    GCCACGGCCA GTGACATGGG TGCCAAGCAG TTCCTGGAGA GTGCACGCAC CGCCGGTGAG
    CTGGCCGATA TGCTCGGAGC AGGCAGTGGC AAGAAGGTGA CCCTCTTCGT GCCCAACGAC
    GCCGCCTTCA TGGAGTACCG TGGTCATCAT CAGCAGGAGA ATAATGTGGA AGATGCCAAG
    GCCGAGGCTT CCAAGCCATC CATCTACAAA CTGCACGCTG TTCTCGGCGA AGTTCAGCTG
    GAGGATGTGC CCAATGAGAA GCTTCTGCAG ACGGAGCTGC CTGACCAGAA GATCCGCATC
    AACAGCTACC AGCTGCCTGC GGCTTTGGGC CTGGAACCCT ACCGCTTTGC CGCCAACTGT
    GTGCCCATCG AGAAGCACGA CAAGCTCTCG GAGCAGGCCC TTGTCCACAC TCTGGGCGGA
    GTCCTGAAGC CGCTGACCAA GAACCTTATG GATATCATCA GGGAGCGTGC GGATATGTCC
    ATTATGCGCA CCGTTCTGGA GAAGACCAAC CTGAGCGCCA TGCTAGAGGA TGACAAGCCA
    GTGACCATCT TTGTGCCCAC CGATGCCGCC TTCGACAAGT TGGAACCTCA TCTGCGTCGA
    GCCCTCAAGG AAGGTCGCGG TTGTGCCTCC AACATCCTGA AGAACCATTT GCTGGACCTC
    ACCTTCTGCT CGCTGGCCAC TGTTCCGGGT GCCAAGACGA CGGCCTACAA TCTACTGGGA
    GAACCCCTGC TCCTCAACCG AACCCACCGC GCTGCGAATC AGACTGGACC GACTCCCATC
    TACATCAATA ACTTGGCCAA AATCATTGAT GCCGATATCA TGGGCACCAA TGGTGTGCTG
    CACGTAATCG ACACCATCCT GCCCACCGAA TCGGCTCTGC CCATGACCTC GCTGATGTCG
    CAAAAGAACC TGACCATCTT CCAGCGATTG CTGGAGGCCT CCGGCTACGA TGATCAGTTC
    GACGATCTGG ACAACGTGAC CATCTTTGCA CCAACTGACA AGGCTCTGCA GAATACGGAA
    TGGGCTCGCA TGCTCAAGGA ACAGCCGGAG CTGCTGAATC ATAACTTGGA TCTGCTGGAG
    TTCCTTAACT ACCATGTGGT CAAGCCCATG ATCAAGACCT GTGACCTGTC GGAGAAGTCC
    CTGCCCACGG TTGCTGGATC TAGTGTGCGT CTGAACCTGT ACTCCACCCA TGCTCTCTTC
    TCGGACGTGA TGAACCGGGC AACGGTAAAC TGTGCCCGTT TGGTGCACTT CGATGACGAG
    AGCTGCGGAT CCGTTCTCCA CCAGGTGGAT CGTGCCTTGG CGCCTCCCAA GAATAACATG
    CTTAAGCTGC TGGAGGCTAA TCCCAACTAC AGCAAGTTCC TGGAGCTGGT TCGTAAGGCT
    AATCTTACAC AGCTGCTGTC CAACGATTCG AGGAGTCTCA CCCTGCTGGT GCCAAAGAAC
    GATATCTTCG AGGAGCTGAA CGAGTCCGGT GAGGGTTCAA AGCCCGCCGA TCCCATGGCC
    TTGGTCAAAA CCCATATCGT TGAGGATGTT GTCTGCTGTG CCGGCATTAT CCCAACCAAC
    TGGCCATTTG TCCGCTCCAT CGAGTCCATC TCTGGCCAGC ATCTGCGTAT CACCCGCGAT
    CGTCGTCCCA AGATCGAGAA CGCCGGCGTC ACCAAGTGCG ATGTGGTGGC CACCAATGGA
    ATTCTCCATG AGATCAACGA CATCATCGTG CCACGCCCAC AGCGCCAGCA ACAGCAGCGA
    CCCCAGCTGA TCCCGCCCGG AGCTGGCTAT CAGCCACAGG GCGATTTCGA TGTCTTCTTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04621
    Clone ID Related Accession (Same CDS sequence) NM_176475.1 , NM_176476.1 , NM_176477.1
    Accession Version NM_176477.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ATTCAAGTTC TGAAGAGGTT GCCGAATTCG ATGACTCCTC GGATTCCACA
    CACGAGTCCT CAGATAACTC CAGCAGCGGA TACGACACCG AGTTCGTGCC ACTGGAGCAC
    CAGCAGCAGC CGCAGGGACG CCAGGAGACG GCGGCCACCG TTCCCGCTCA AACCCCCAGC
    GGTGTGGAGC AGAAGCCGTT CAATGTGGAC ACCATCACCA CGGATGTGAA TTCCCCAAAT
    CCGGCCATCT TCTTCCAGCA ATCGTTCCCC TTCTTTGGCA ATGAGTTCTT CAATTCGTTC
    GGAGGCTTTG GCTTTGGAGC TGCCCAGGAG CCCTGGTGGA AGGGACCCAA TGTGTGCACC
    GAAAAGGAGG AAGACGAGAC TGTGGCCAGT GAAACGGAGG CAGAAGAAAC CGTGGACACC
    TTTGGCCAGG AACGTGATGG CGTTACCAAC GTGGTGCGAT CCCCACTCTT TGGCCAGTTC
    CAGTTCAGCG TTAACTCCTG CGCCGAGAAG CCCAACAAGC ACGTCTGCAC CAAGATCGTG
    AATCAAAATG GCAAGAAGAA AACGCTGACG CTGACCCGCC AGTGCTGCTA TGGATATGGA
    CGCCCCAGAA ACGCTGACTT CACAACGCCC TGCGAGAAGA TCGACATCAA GGACGTGGAG
    GCCACGGCCA GTGACATGGG TGCCAAGCAG TTCCTGGAGA GTGCACGCAC CGCCGGTGAG
    CTGGCCGATA TGCTCGGAGC AGGCAGTGGC AAGAAGGTGA CCCTCTTCGT GCCCAACGAC
    GCCGCCTTCA TGGAGTACCG TGGTCATCAT CAGCAGGAGA ATAATGTGGA AGATGCCAAG
    GCCGAGGCTT CCAAGCCATC CATCTACAAA CTGCACGCTG TTCTCGGCGA AGTTCAGCTG
    GAGGATGTGC CCAATGAGAA GCTTCTGCAG ACGGAGCTGC CTGACCAGAA GATCCGCATC
    AACAGCTACC AGCTGCCTGC GGCTTTGGGC CTGGAACCCT ACCGCTTTGC CGCCAACTGT
    GTGCCCATCG AGAAGCACGA CAAGCTCTCG GAGCAGGCCC TTGTCCACAC TCTGGGCGGA
    GTCCTGAAGC CGCTGACCAA GAACCTTATG GATATCATCA GGGAGCGTGC GGATATGTCC
    ATTATGCGCA CCGTTCTGGA GAAGACCAAC CTGAGCGCCA TGCTAGAGGA TGACAAGCCA
    GTGACCATCT TTGTGCCCAC CGATGCCGCC TTCGACAAGT TGGAACCTCA TCTGCGTCGA
    GCCCTCAAGG AAGGTCGCGG TTGTGCCTCC AACATCCTGA AGAACCATTT GCTGGACCTC
    ACCTTCTGCT CGCTGGCCAC TGTTCCGGGT GCCAAGACGA CGGCCTACAA TCTACTGGGA
    GAACCCCTGC TCCTCAACCG AACCCACCGC GCTGCGAATC AGACTGGACC GACTCCCATC
    TACATCAATA ACTTGGCCAA AATCATTGAT GCCGATATCA TGGGCACCAA TGGTGTGCTG
    CACGTAATCG ACACCATCCT GCCCACCGAA TCGGCTCTGC CCATGACCTC GCTGATGTCG
    CAAAAGAACC TGACCATCTT CCAGCGATTG CTGGAGGCCT CCGGCTACGA TGATCAGTTC
    GACGATCTGG ACAACGTGAC CATCTTTGCA CCAACTGACA AGGCTCTGCA GAATACGGAA
    TGGGCTCGCA TGCTCAAGGA ACAGCCGGAG CTGCTGAATC ATAACTTGGA TCTGCTGGAG
    TTCCTTAACT ACCATGTGGT CAAGCCCATG ATCAAGACCT GTGACCTGTC GGAGAAGTCC
    CTGCCCACGG TTGCTGGATC TAGTGTGCGT CTGAACCTGT ACTCCACCCA TGCTCTCTTC
    TCGGACGTGA TGAACCGGGC AACGGTAAAC TGTGCCCGTT TGGTGCACTT CGATGACGAG
    AGCTGCGGAT CCGTTCTCCA CCAGGTGGAT CGTGCCTTGG CGCCTCCCAA GAATAACATG
    CTTAAGCTGC TGGAGGCTAA TCCCAACTAC AGCAAGTTCC TGGAGCTGGT TCGTAAGGCT
    AATCTTACAC AGCTGCTGTC CAACGATTCG AGGAGTCTCA CCCTGCTGGT GCCAAAGAAC
    GATATCTTCG AGGAGCTGAA CGAGTCCGGT GAGGGTTCAA AGCCCGCCGA TCCCATGGCC
    TTGGTCAAAA CCCATATCGT TGAGGATGTT GTCTGCTGTG CCGGCATTAT CCCAACCAAC
    TGGCCATTTG TCCGCTCCAT CGAGTCCATC TCTGGCCAGC ATCTGCGTAT CACCCGCGAT
    CGTCGTCCCA AGATCGAGAA CGCCGGCGTC ACCAAGTGCG ATGTGGTGGC CACCAATGGA
    ATTCTCCATG AGATCAACGA CATCATCGTG CCACGCCCAC AGCGCCAGCA ACAGCAGCGA
    CCCCAGCTGA TCCCGCCCGG AGCTGGCTAT CAGCCACAGG GCGATTTCGA TGTCTTCTTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788654.1
    CDS130..2652
    Translation

    Target ORF information:

    RefSeq Version NM_176477.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant M, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176477.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ATTCAAGTTC TGAAGAGGTT GCCGAATTCG ATGACTCCTC GGATTCCACA
    CACGAGTCCT CAGATAACTC CAGCAGCGGA TACGACACCG AGTTCGTGCC ACTGGAGCAC
    CAGCAGCAGC CGCAGGGACG CCAGGAGACG GCGGCCACCG TTCCCGCTCA AACCCCCAGC
    GGTGTGGAGC AGAAGCCGTT CAATGTGGAC ACCATCACCA CGGATGTGAA TTCCCCAAAT
    CCGGCCATCT TCTTCCAGCA ATCGTTCCCC TTCTTTGGCA ATGAGTTCTT CAATTCGTTC
    GGAGGCTTTG GCTTTGGAGC TGCCCAGGAG CCCTGGTGGA AGGGACCCAA TGTGTGCACC
    GAAAAGGAGG AAGACGAGAC TGTGGCCAGT GAAACGGAGG CAGAAGAAAC CGTGGACACC
    TTTGGCCAGG AACGTGATGG CGTTACCAAC GTGGTGCGAT CCCCACTCTT TGGCCAGTTC
    CAGTTCAGCG TTAACTCCTG CGCCGAGAAG CCCAACAAGC ACGTCTGCAC CAAGATCGTG
    AATCAAAATG GCAAGAAGAA AACGCTGACG CTGACCCGCC AGTGCTGCTA TGGATATGGA
    CGCCCCAGAA ACGCTGACTT CACAACGCCC TGCGAGAAGA TCGACATCAA GGACGTGGAG
    GCCACGGCCA GTGACATGGG TGCCAAGCAG TTCCTGGAGA GTGCACGCAC CGCCGGTGAG
    CTGGCCGATA TGCTCGGAGC AGGCAGTGGC AAGAAGGTGA CCCTCTTCGT GCCCAACGAC
    GCCGCCTTCA TGGAGTACCG TGGTCATCAT CAGCAGGAGA ATAATGTGGA AGATGCCAAG
    GCCGAGGCTT CCAAGCCATC CATCTACAAA CTGCACGCTG TTCTCGGCGA AGTTCAGCTG
    GAGGATGTGC CCAATGAGAA GCTTCTGCAG ACGGAGCTGC CTGACCAGAA GATCCGCATC
    AACAGCTACC AGCTGCCTGC GGCTTTGGGC CTGGAACCCT ACCGCTTTGC CGCCAACTGT
    GTGCCCATCG AGAAGCACGA CAAGCTCTCG GAGCAGGCCC TTGTCCACAC TCTGGGCGGA
    GTCCTGAAGC CGCTGACCAA GAACCTTATG GATATCATCA GGGAGCGTGC GGATATGTCC
    ATTATGCGCA CCGTTCTGGA GAAGACCAAC CTGAGCGCCA TGCTAGAGGA TGACAAGCCA
    GTGACCATCT TTGTGCCCAC CGATGCCGCC TTCGACAAGT TGGAACCTCA TCTGCGTCGA
    GCCCTCAAGG AAGGTCGCGG TTGTGCCTCC AACATCCTGA AGAACCATTT GCTGGACCTC
    ACCTTCTGCT CGCTGGCCAC TGTTCCGGGT GCCAAGACGA CGGCCTACAA TCTACTGGGA
    GAACCCCTGC TCCTCAACCG AACCCACCGC GCTGCGAATC AGACTGGACC GACTCCCATC
    TACATCAATA ACTTGGCCAA AATCATTGAT GCCGATATCA TGGGCACCAA TGGTGTGCTG
    CACGTAATCG ACACCATCCT GCCCACCGAA TCGGCTCTGC CCATGACCTC GCTGATGTCG
    CAAAAGAACC TGACCATCTT CCAGCGATTG CTGGAGGCCT CCGGCTACGA TGATCAGTTC
    GACGATCTGG ACAACGTGAC CATCTTTGCA CCAACTGACA AGGCTCTGCA GAATACGGAA
    TGGGCTCGCA TGCTCAAGGA ACAGCCGGAG CTGCTGAATC ATAACTTGGA TCTGCTGGAG
    TTCCTTAACT ACCATGTGGT CAAGCCCATG ATCAAGACCT GTGACCTGTC GGAGAAGTCC
    CTGCCCACGG TTGCTGGATC TAGTGTGCGT CTGAACCTGT ACTCCACCCA TGCTCTCTTC
    TCGGACGTGA TGAACCGGGC AACGGTAAAC TGTGCCCGTT TGGTGCACTT CGATGACGAG
    AGCTGCGGAT CCGTTCTCCA CCAGGTGGAT CGTGCCTTGG CGCCTCCCAA GAATAACATG
    CTTAAGCTGC TGGAGGCTAA TCCCAACTAC AGCAAGTTCC TGGAGCTGGT TCGTAAGGCT
    AATCTTACAC AGCTGCTGTC CAACGATTCG AGGAGTCTCA CCCTGCTGGT GCCAAAGAAC
    GATATCTTCG AGGAGCTGAA CGAGTCCGGT GAGGGTTCAA AGCCCGCCGA TCCCATGGCC
    TTGGTCAAAA CCCATATCGT TGAGGATGTT GTCTGCTGTG CCGGCATTAT CCCAACCAAC
    TGGCCATTTG TCCGCTCCAT CGAGTCCATC TCTGGCCAGC ATCTGCGTAT CACCCGCGAT
    CGTCGTCCCA AGATCGAGAA CGCCGGCGTC ACCAAGTGCG ATGTGGTGGC CACCAATGGA
    ATTCTCCATG AGATCAACGA CATCATCGTG CCACGCCCAC AGCGCCAGCA ACAGCAGCGA
    CCCCAGCTGA TCCCGCCCGG AGCTGGCTAT CAGCCACAGG GCGATTTCGA TGTCTTCTTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04622
    Clone ID Related Accession (Same CDS sequence) NM_176478.1
    Accession Version NM_176478.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ATTCAAGTTC TGAAGAGGTT GCCGAATTCG ATGACTCCTC GGATTCCACA
    CACGAGTCCT CAGATAACTC CAGCTTTATG CCACATTTAA CCCACCCATT GCCGGCGCAT
    CGCAATCTGT TCGCGCCCCT GCCCTTCGCA CCACTCGCAC CGCTCGAGCC CCAAGATCCA
    CTGCATGCCC AGGCCTCGCA GCGGATGGCG AGCGTCTGGA ACGGACCCGC CCCGTTGGCG
    CCGCAACTCC ACTCATCTCA CCTGTTGCCC CTCTTCAGCA GCGGATACGA CACCGAGTTC
    GTGCCACTGG AGCACCAGCA GCAGCCGCAG GGACGCCAGG AGACGGCGGC CACCGTTCCC
    GCTCAAACCC CCAGCGGTGT GGAGCAGAAG CCGTTCAATG TGGACACCAT CACCACGGAT
    GTGAATTCCC CAAATCCGGC CATCTTCTTC CAGCAATCGT TCCCCTTCTT TGGCAATGAG
    TTCTTCAATT CGTTCGGAGG CTTTGGCTTT GGAGCTGCCC AGGAGCCCTG GTGGAAGGGA
    CCCAATGTGT GCACCGAAAA GGAGGAAGAC GAGACTGTGG CCAGTGAAAC GGAGGCAGAA
    GAAACCGTGG ACACCTTTGG CCAGGAACGT GATGGCGTTA CCAACGTGGT GCGATCCCCA
    CTCTTTGGCC AGTTCCAGTT CAGCGTTAAC TCCTGCGCCG AGAAGCCCAA CAAGCACGTC
    TGCACCAAGA TCGTGAATCA AAATGGCAAG AAGAAAACGC TGACGCTGAC CCGCCAGTGC
    TGCTATGGAT ATGGACGCCC CAGAAACGCT GACTTCACAA CGCCCTGCGA GAAGATCGAC
    ATCAAGGACG TGGAGGCCAC GGCCAGTGAC ATGGGTGCCA AGCAGTTCCT GGAGAGTGCA
    CGCACCGCCG GTGAGCTGGC CGATATGCTC GGAGCAGGCA GTGGCAAGAA GGTGACCCTC
    TTCGTGCCCA ACGACGCCGC CTTCATGGAG TACCGTGGTC ATCATCAGCA GGAGAATAAT
    GTGGAAGATG CCAAGGCCGA GGCTTCCAAG CCATCCATCT ACAAACTGCA CGCTGTTCTC
    GGCGAAGTTC AGCTGGAGGA TGTGCCCAAT GAGAAGCTTC TGCAGACGGA GCTGCCTGAC
    CAGAAGATCC GCATCAACAG CTACCAGCTG CCTGCGGCTT TGGGCCTGGA ACCCTACCGC
    TTTGCCGCCA ACTGTGTGCC CATCGAGAAG CACGACAAGC TCTCGGAGCA GGCCCTTGTC
    CACACTCTGG GCGGAGTCCT GAAGCCGCTG ACCAAGAACC TTATGGATAT CATCAGGGAG
    CGTGCGGATA TGTCCATTAT GCGCACCGTT CTGGAGAAGA CCAACCTGAG CGCCATGCTA
    GAGGATGACA AGCCAGTGAC CATCTTTGTG CCCACCGATG CCGCCTTCGA CAAGTTGGAA
    CCTCATCTGC GTCGAGCCCT CAAGGAAGGT CGCGGTTGTG CCTCCAACAT CCTGAAGAAC
    CATTTGCTGG ACCTCACCTT CTGCTCGCTG GCCACTGTTC CGGGTGCCAA GACGACGGCC
    TACAATCTAC TGGGAGAACC CCTGCTCCTC AACCGAACCC ACCGCGCTGC GAATCAGACT
    GGACCGACTC CCATCTACAT CAATAACTTG GCCAAAATCA TTGATGCCGA TATCATGGGC
    ACCAATGGTG TGCTGCACGT AATCGACACC ATCCTGCCCA CCGAATCGGC TCTGCCCATG
    ACCTCGCTGA TGTCGCAAAA GAACCTGACC ATCTTCCAGC GATTGCTGGA GGCCTCCGGC
    TACGATGATC AGTTCGACGA TCTGGACAAC GTGACCATCT TTGCACCAAC TGACAAGGCT
    CTGCAGAATA CGGAATGGGC TCGCATGCTC AAGGAACAGC CGGAGCTGCT GAATCATAAC
    TTGGATCTGC TGGAGTTCCT TAACTACCAT GTGGTCAAGC CCATGATCAA GACCTGTGAC
    CTGTCGGAGA AGTCCCTGCC CACGGTTGCT GGATCTAGTG TGCGTCTGAA CCTGTACTCC
    ACCCATGCTC TCTTCTCGGA CGTGATGAAC CGGGCAACGG TAAACTGTGC CCGTTTGGTG
    CACTTCGATG ACGAGAGCTG CGGATCCGTT CTCCACCAGG TGGATCGTGC CTTGGCGCCT
    CCCAAGAATA ACATGCTTAA GCTGCTGGAG GCTAATCCCA ACTACAGCAA GTTCCTGGAG
    CTGGTTCGTA AGGCTAATCT TACACAGCTG CTGTCCAACG ATTCGAGGAG TCTCACCCTG
    CTGGTGCCAA AGAACGATAT CTTCGAGGAG CTGAACGAGT CCGGTGAGGG TTCAAAGCCC
    GCCGATCCCA TGGCCTTGGT CAAAACCCAT ATCGTTGAGG ATGTTGTCTG CTGTGCCGGC
    ATTATCCCAA CCAACTGGCC ATTTGTCCGC TCCATCGAGT CCATCTCTGG CCAGCATCTG
    CGTATCACCC GCGATCGTCG TCCCAAGATC GAGAACGCCG GCGTCACCAA GTGCGATGTG
    GTGGCCACCA ATGGAATTCT CCATGAGATC AACGACATCA TCGTGCCACG CCCACAGCGC
    CAGCAACAGC AGCGACCCCA GCTGATCCCG CCCGGAGCTG GCTATCAGCC ACAGGGCGAT
    TTCGATGTCT TCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788655.1
    CDS108..2825
    Translation

    Target ORF information:

    RefSeq Version NM_176478.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176478.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTACGGC TGTGGGCCTG CCTGCTCCTC CTGGGATCAA TCCAGATCCA GGCGGTTCCA 
    TTCTACGGCG ATTCAAGTTC TGAAGAGGTT GCCGAATTCG ATGACTCCTC GGATTCCACA
    CACGAGTCCT CAGATAACTC CAGCTTTATG CCACATTTAA CCCACCCATT GCCGGCGCAT
    CGCAATCTGT TCGCGCCCCT GCCCTTCGCA CCACTCGCAC CGCTCGAGCC CCAAGATCCA
    CTGCATGCCC AGGCCTCGCA GCGGATGGCG AGCGTCTGGA ACGGACCCGC CCCGTTGGCG
    CCGCAACTCC ACTCATCTCA CCTGTTGCCC CTCTTCAGCA GCGGATACGA CACCGAGTTC
    GTGCCACTGG AGCACCAGCA GCAGCCGCAG GGACGCCAGG AGACGGCGGC CACCGTTCCC
    GCTCAAACCC CCAGCGGTGT GGAGCAGAAG CCGTTCAATG TGGACACCAT CACCACGGAT
    GTGAATTCCC CAAATCCGGC CATCTTCTTC CAGCAATCGT TCCCCTTCTT TGGCAATGAG
    TTCTTCAATT CGTTCGGAGG CTTTGGCTTT GGAGCTGCCC AGGAGCCCTG GTGGAAGGGA
    CCCAATGTGT GCACCGAAAA GGAGGAAGAC GAGACTGTGG CCAGTGAAAC GGAGGCAGAA
    GAAACCGTGG ACACCTTTGG CCAGGAACGT GATGGCGTTA CCAACGTGGT GCGATCCCCA
    CTCTTTGGCC AGTTCCAGTT CAGCGTTAAC TCCTGCGCCG AGAAGCCCAA CAAGCACGTC
    TGCACCAAGA TCGTGAATCA AAATGGCAAG AAGAAAACGC TGACGCTGAC CCGCCAGTGC
    TGCTATGGAT ATGGACGCCC CAGAAACGCT GACTTCACAA CGCCCTGCGA GAAGATCGAC
    ATCAAGGACG TGGAGGCCAC GGCCAGTGAC ATGGGTGCCA AGCAGTTCCT GGAGAGTGCA
    CGCACCGCCG GTGAGCTGGC CGATATGCTC GGAGCAGGCA GTGGCAAGAA GGTGACCCTC
    TTCGTGCCCA ACGACGCCGC CTTCATGGAG TACCGTGGTC ATCATCAGCA GGAGAATAAT
    GTGGAAGATG CCAAGGCCGA GGCTTCCAAG CCATCCATCT ACAAACTGCA CGCTGTTCTC
    GGCGAAGTTC AGCTGGAGGA TGTGCCCAAT GAGAAGCTTC TGCAGACGGA GCTGCCTGAC
    CAGAAGATCC GCATCAACAG CTACCAGCTG CCTGCGGCTT TGGGCCTGGA ACCCTACCGC
    TTTGCCGCCA ACTGTGTGCC CATCGAGAAG CACGACAAGC TCTCGGAGCA GGCCCTTGTC
    CACACTCTGG GCGGAGTCCT GAAGCCGCTG ACCAAGAACC TTATGGATAT CATCAGGGAG
    CGTGCGGATA TGTCCATTAT GCGCACCGTT CTGGAGAAGA CCAACCTGAG CGCCATGCTA
    GAGGATGACA AGCCAGTGAC CATCTTTGTG CCCACCGATG CCGCCTTCGA CAAGTTGGAA
    CCTCATCTGC GTCGAGCCCT CAAGGAAGGT CGCGGTTGTG CCTCCAACAT CCTGAAGAAC
    CATTTGCTGG ACCTCACCTT CTGCTCGCTG GCCACTGTTC CGGGTGCCAA GACGACGGCC
    TACAATCTAC TGGGAGAACC CCTGCTCCTC AACCGAACCC ACCGCGCTGC GAATCAGACT
    GGACCGACTC CCATCTACAT CAATAACTTG GCCAAAATCA TTGATGCCGA TATCATGGGC
    ACCAATGGTG TGCTGCACGT AATCGACACC ATCCTGCCCA CCGAATCGGC TCTGCCCATG
    ACCTCGCTGA TGTCGCAAAA GAACCTGACC ATCTTCCAGC GATTGCTGGA GGCCTCCGGC
    TACGATGATC AGTTCGACGA TCTGGACAAC GTGACCATCT TTGCACCAAC TGACAAGGCT
    CTGCAGAATA CGGAATGGGC TCGCATGCTC AAGGAACAGC CGGAGCTGCT GAATCATAAC
    TTGGATCTGC TGGAGTTCCT TAACTACCAT GTGGTCAAGC CCATGATCAA GACCTGTGAC
    CTGTCGGAGA AGTCCCTGCC CACGGTTGCT GGATCTAGTG TGCGTCTGAA CCTGTACTCC
    ACCCATGCTC TCTTCTCGGA CGTGATGAAC CGGGCAACGG TAAACTGTGC CCGTTTGGTG
    CACTTCGATG ACGAGAGCTG CGGATCCGTT CTCCACCAGG TGGATCGTGC CTTGGCGCCT
    CCCAAGAATA ACATGCTTAA GCTGCTGGAG GCTAATCCCA ACTACAGCAA GTTCCTGGAG
    CTGGTTCGTA AGGCTAATCT TACACAGCTG CTGTCCAACG ATTCGAGGAG TCTCACCCTG
    CTGGTGCCAA AGAACGATAT CTTCGAGGAG CTGAACGAGT CCGGTGAGGG TTCAAAGCCC
    GCCGATCCCA TGGCCTTGGT CAAAACCCAT ATCGTTGAGG ATGTTGTCTG CTGTGCCGGC
    ATTATCCCAA CCAACTGGCC ATTTGTCCGC TCCATCGAGT CCATCTCTGG CCAGCATCTG
    CGTATCACCC GCGATCGTCG TCCCAAGATC GAGAACGCCG GCGTCACCAA GTGCGATGTG
    GTGGCCACCA ATGGAATTCT CCATGAGATC AACGACATCA TCGTGCCACG CCCACAGCGC
    CAGCAACAGC AGCGACCCCA GCTGATCCCG CCCGGAGCTG GCTATCAGCC ACAGGGCGAT
    TTCGATGTCT TCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08330
    Clone ID Related Accession (Same CDS sequence) NM_001260137.1
    Accession Version NM_001260137.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product midline fasciclin, isoform Q
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCGGCTCA GCAGCGGATA CGACACCGAG TTCGTGCCAC TGGAGCACCA GCAGCAGCCG 
    CAGGGACGCC AGGAGACGGC GGCCACCGTT CCCGCTCAAA CCCCCAGCGG TGTGGAGCAG
    AAGCCGTTCA ATGTGGACAC CATCACCACG GATGTGAATT CCCCAAATCC GGCCATCTTC
    TTCCAGCAAT CGTTCCCCTT CTTTGGCAAT GAGTTCTTCA ATTCGTTCGG AGGCTTTGGC
    TTTGGAGCTG CCCAGGAGCC CTGGTGGAAG GGACCCAATG TGTGCACCGA AAAGGAGGAA
    GACGAGACTG TGGCCAGTGA AACGGAGGCA GAAGAAACCG TGGACACCTT TGGCCAGGAA
    CGTGATGGCG TTACCAACGT GGTGCGATCC CCACTCTTTG GCCAGTTCCA GTTCAGCGTT
    AACTCCTGCG CCGAGAAGCC CAACAAGCAC GTCTGCACCA AGATCGTGAA TCAAAATGGC
    AAGAAGAAAA CGCTGACGCT GACCCGCCAG TGCTGCTATG GATATGGACG CCCCAGAAAC
    GCTGACTTCA CAACGCCCTG CGAGAAGATC GACATCAAGG ACGTGGAGGC CACGGCCAGT
    GACATGGGTG CCAAGCAGTT CCTGGAGAGT GCACGCACCG CCGGTGAGCT GGCCGATATG
    CTCGGAGCAG GCAGTGGCAA GAAGGTGACC CTCTTCGTGC CCAACGACGC CGCCTTCATG
    GAGTACCGTG GTCATCATCA GCAGGAGAAT AATGTGGAAG ATGCCAAGGC CGAGGCTTCC
    AAGCCATCCA TCTACAAACT GCACGCTGTT CTCGGCGAAG TTCAGCTGGA GGATGTGCCC
    AATGAGAAGC TTCTGCAGAC GGAGCTGCCT GACCAGAAGA TCCGCATCAA CAGCTACCAG
    CTGCCTGCGG CTTTGGGCCT GGAACCCTAC CGCTTTGCCG CCAACTGTGT GCCCATCGAG
    AAGCACGACA AGCTCTCGGA GCAGGCCCTT GTCCACACTC TGGGCGGAGT CCTGAAGCCG
    CTGACCAAGA ACCTTATGGA TATCATCAGG GAGCGTGCGG ATATGTCCAT TATGCGCACC
    GTTCTGGAGA AGACCAACCT GAGCGCCATG CTAGAGGATG ACAAGCCAGT GACCATCTTT
    GTGCCCACCG ATGCCGCCTT CGACAAGTTG GAACCTCATC TGCGTCGAGC CCTCAAGGAA
    GGTCGCGGTT GTGCCTCCAA CATCCTGAAG AACCATTTGC TGGACCTCAC CTTCTGCTCG
    CTGGCCACTG TTCCGGGTGC CAAGACGACG GCCTACAATC TACTGGGAGA ACCCCTGCTC
    CTCAACCGAA CCCACCGCGC TGCGAATCAG ACTGGACCGA CTCCCATCTA CATCAATAAC
    TTGGCCAAAA TCATTGATGC CGATATCATG GGCACCAATG GTGTGCTGCA CGTAATCGAC
    ACCATCCTGC CCACCGAATC GGCTCTGCCC ATGACCTCGC TGATGTCGCA AAAGAACCTG
    ACCATCTTCC AGCGATTGCT GGAGGCCTCC GGCTACGATG ATCAGTTCGA CGATCTGGAC
    AACGTGACCA TCTTTGCACC AACTGACAAG GCTCTGCAGA ATACGGAATG GGCTCGCATG
    CTCAAGGAAC AGCCGGAGCT GCTGAATCAT AACTTGGATC TGCTGGAGTT CCTTAACTAC
    CATGTGGTCA AGCCCATGAT CAAGACCTGT GACCTGTCGG AGAAGTCCCT GCCCACGGTT
    GCTGGATCTA GTGTGCGTCT GAACCTGTAC TCCACCCATG CTCTCTTCTC GGACGTGATG
    AACCGGGCAA CGGTAAACTG TGCCCGTTTG GTGCACTTCG ATGACGAGAG CTGCGGATCC
    GTTCTCCACC AGGTGGATCG TGCCTTGGCG CCTCCCAAGA ATAACATGCT TAAGCTGCTG
    GAGGCTAATC CCAACTACAG CAAGTTCCTG GAGCTGGTTC GTAAGGCTAA TCTTACACAG
    CTGCTGTCCA ACGATTCGAG GAGTCTCACC CTGCTGGTGC CAAAGAACGA TATCTTCGAG
    GAGCTGAACG AGTCCGGTGA GGGTTCAAAG CCCGCCGATC CCATGGCCTT GGTCAAAACC
    CATATCGTTG AGGATGTTGT CTGCTGTGCC GGCATTATCC CAACCAACTG GCCATTTGTC
    CGCTCCATCG AGTCCATCTC TGGCCAGCAT CTGCGTATCA CCCGCGATCG TCGTCCCAAG
    ATCGAGAACG CCGGCGTCAC CAAGTGCGAT GTGGTGGCCA CCAATGGAAT TCTCCATGAG
    ATCAACGACA TCATCGTGCC ACGCCCACAG CGCCAGCAAC AGCAGCGACC CCAGCTGATC
    CCGCCCGGAG CTGGCTATCA GCCACAGGGC GATTTCGATG TCTTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247066.1
    CDS150..2540
    Translation

    Target ORF information:

    RefSeq Version NM_001260137.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster midline fasciclin (mfas), transcript variant Q, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001260137.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCGGCTCA GCAGCGGATA CGACACCGAG TTCGTGCCAC TGGAGCACCA GCAGCAGCCG 
    CAGGGACGCC AGGAGACGGC GGCCACCGTT CCCGCTCAAA CCCCCAGCGG TGTGGAGCAG
    AAGCCGTTCA ATGTGGACAC CATCACCACG GATGTGAATT CCCCAAATCC GGCCATCTTC
    TTCCAGCAAT CGTTCCCCTT CTTTGGCAAT GAGTTCTTCA ATTCGTTCGG AGGCTTTGGC
    TTTGGAGCTG CCCAGGAGCC CTGGTGGAAG GGACCCAATG TGTGCACCGA AAAGGAGGAA
    GACGAGACTG TGGCCAGTGA AACGGAGGCA GAAGAAACCG TGGACACCTT TGGCCAGGAA
    CGTGATGGCG TTACCAACGT GGTGCGATCC CCACTCTTTG GCCAGTTCCA GTTCAGCGTT
    AACTCCTGCG CCGAGAAGCC CAACAAGCAC GTCTGCACCA AGATCGTGAA TCAAAATGGC
    AAGAAGAAAA CGCTGACGCT GACCCGCCAG TGCTGCTATG GATATGGACG CCCCAGAAAC
    GCTGACTTCA CAACGCCCTG CGAGAAGATC GACATCAAGG ACGTGGAGGC CACGGCCAGT
    GACATGGGTG CCAAGCAGTT CCTGGAGAGT GCACGCACCG CCGGTGAGCT GGCCGATATG
    CTCGGAGCAG GCAGTGGCAA GAAGGTGACC CTCTTCGTGC CCAACGACGC CGCCTTCATG
    GAGTACCGTG GTCATCATCA GCAGGAGAAT AATGTGGAAG ATGCCAAGGC CGAGGCTTCC
    AAGCCATCCA TCTACAAACT GCACGCTGTT CTCGGCGAAG TTCAGCTGGA GGATGTGCCC
    AATGAGAAGC TTCTGCAGAC GGAGCTGCCT GACCAGAAGA TCCGCATCAA CAGCTACCAG
    CTGCCTGCGG CTTTGGGCCT GGAACCCTAC CGCTTTGCCG CCAACTGTGT GCCCATCGAG
    AAGCACGACA AGCTCTCGGA GCAGGCCCTT GTCCACACTC TGGGCGGAGT CCTGAAGCCG
    CTGACCAAGA ACCTTATGGA TATCATCAGG GAGCGTGCGG ATATGTCCAT TATGCGCACC
    GTTCTGGAGA AGACCAACCT GAGCGCCATG CTAGAGGATG ACAAGCCAGT GACCATCTTT
    GTGCCCACCG ATGCCGCCTT CGACAAGTTG GAACCTCATC TGCGTCGAGC CCTCAAGGAA
    GGTCGCGGTT GTGCCTCCAA CATCCTGAAG AACCATTTGC TGGACCTCAC CTTCTGCTCG
    CTGGCCACTG TTCCGGGTGC CAAGACGACG GCCTACAATC TACTGGGAGA ACCCCTGCTC
    CTCAACCGAA CCCACCGCGC TGCGAATCAG ACTGGACCGA CTCCCATCTA CATCAATAAC
    TTGGCCAAAA TCATTGATGC CGATATCATG GGCACCAATG GTGTGCTGCA CGTAATCGAC
    ACCATCCTGC CCACCGAATC GGCTCTGCCC ATGACCTCGC TGATGTCGCA AAAGAACCTG
    ACCATCTTCC AGCGATTGCT GGAGGCCTCC GGCTACGATG ATCAGTTCGA CGATCTGGAC
    AACGTGACCA TCTTTGCACC AACTGACAAG GCTCTGCAGA ATACGGAATG GGCTCGCATG
    CTCAAGGAAC AGCCGGAGCT GCTGAATCAT AACTTGGATC TGCTGGAGTT CCTTAACTAC
    CATGTGGTCA AGCCCATGAT CAAGACCTGT GACCTGTCGG AGAAGTCCCT GCCCACGGTT
    GCTGGATCTA GTGTGCGTCT GAACCTGTAC TCCACCCATG CTCTCTTCTC GGACGTGATG
    AACCGGGCAA CGGTAAACTG TGCCCGTTTG GTGCACTTCG ATGACGAGAG CTGCGGATCC
    GTTCTCCACC AGGTGGATCG TGCCTTGGCG CCTCCCAAGA ATAACATGCT TAAGCTGCTG
    GAGGCTAATC CCAACTACAG CAAGTTCCTG GAGCTGGTTC GTAAGGCTAA TCTTACACAG
    CTGCTGTCCA ACGATTCGAG GAGTCTCACC CTGCTGGTGC CAAAGAACGA TATCTTCGAG
    GAGCTGAACG AGTCCGGTGA GGGTTCAAAG CCCGCCGATC CCATGGCCTT GGTCAAAACC
    CATATCGTTG AGGATGTTGT CTGCTGTGCC GGCATTATCC CAACCAACTG GCCATTTGTC
    CGCTCCATCG AGTCCATCTC TGGCCAGCAT CTGCGTATCA CCCGCGATCG TCGTCCCAAG
    ATCGAGAACG CCGGCGTCAC CAAGTGCGAT GTGGTGGCCA CCAATGGAAT TCTCCATGAG
    ATCAACGACA TCATCGTGCC ACGCCCACAG CGCCAGCAAC AGCAGCGACC CCAGCTGATC
    CCGCCCGGAG CTGGCTATCA GCCACAGGGC GATTTCGATG TCTTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.