hb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hb
Entrez Gene ID	41032
Full Name	hunchback
Synonyms	CG9786,Dmel\CG9786,HB,Hb,Hunchback,R-pbx,Rg-bx,Rg-pbx,hbHLH,l(3)85Ah
General protein information	Preferred Names hunchback Names CG9786 gene product from transcript CG9786-RA CG9786-PA CG9786-PB Regulator-of-postbithorax hb-PA hb-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85A5-85A5\|3-48 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_169234.2	NP_731268.1	hunchback, isoform B
NM_169233.2	NP_731267.1	hunchback, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010777	XP_318530.4
Drosophila melanogaster (fruit fly)	hb	NP_731267.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In this study, ChIP/chip datasets are analyzed using the corresponding PWMs for the well-studied TFs; CAUDAL, HUNCHBACK, KNIRPS and KRUPPEL, to determine the distribution of predicted binding sites. All four TFs are critical regulators of gene expression along the anterio-posterior axis in early Drosophila development
Title: Spatial distribution of predicted transcription factor binding sites in Drosophila ChIP peaks.

Authors developed a simple model of hb transcription mid-blastula transition (MBT); model provides a clear dynamical picture of hb transcription regulation as one of the many, actively regulated events concurrently taking place during the MBT.
Title: Time to move on: Modeling transcription dynamics during an embryonic transition away from maternal control.

Hb bifunctionally regulates eve stripe 7, but it executes these two activities on different pieces of regulatory DNA--it activates the eve2+7 enhancer and represses the eve3+7 enhancer
Title: Shadow enhancers enable Hunchback bifunctionality in the Drosophila embryo.

Kr regulation can limit the positional variability of the Hb mid-embryo border.
Title: Mid-embryo patterning and precision in Drosophila segmentation: Kr?ppel dual regulation of hunchback.

The expression of Hunchback in the neuroblast increases over time and the absolute levels of its expression specify two different Crustacean-cardioactive-peptide neuronal fates.
Title: Specification of neuronal subtypes by different levels of Hunchback.

The following hb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04254	NM_169233.2 Latest version!	Drosophila melanogaster hunchback (hb), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa04254	NM_169234.2 Latest version!	Drosophila melanogaster hunchback (hb), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hb ( NM_169234.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731268.1 Drosophila melanogaster hunchback (hb), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04254
Clone ID Related Accession (Same CDS sequence)	NM_169234.2 , NM_169233.2
Accession Version	NM_169234.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hunchback, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169234.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAACT GGGAGACGAC AGCCACGACC AACTACGAGC AGCACAACGC CTGGTACAAC 
AGCATGTTCG CGGCAAATAT CAAACAGGAG CCAGGTCATC ATCTCGACGG GAATAGCGTG 
GCCAGCAGTC CGCGCCAATC GCCCATTCCC TCGACCAATC ACCTGGAACA GTTCCTCAAG 
CAGCAGCAGC AGCAGCTTCA GCAGCAACCC ATGGATACCC TGTGCGCCAT GACCCCATCA 
CCCAGCCAAA ACGATCAAAA CAGCCTGCAG CATTACGATG CTAACTTGCA GCAACAGTTG 
CTGCAGCAAC AGCAGTACCA GCAGCATTTC CAGGCAGCCC AGCAGCAACA TCATCACCAT 
CACCATCTGA TGGGTGGATT CAATCCGCTG ACGCCACCTG GTCTGCCCAA TCCCATGCAG 
CACTTCTATG GCGGCAATCT GCGACCCAGT CCGCAGCCCA CGCCCACATC TGCCTCCACA 
ATTGCGCCCG TTGCAGTTGC CACTGGCAGC AGCGAGAAGT TGCAGGCACT AACACCACCC 
ATGGATGTCA CACCGCCTAA GTCGCCGGCC AAGTCGAGTC AGTCGAATAT TGAGCCGGAG 
AAGGAGCACG ATCAGATGTC GAACTCCAGC GAGGACATGA AGTACATGGC CGAGTCCGAG 
GACGATGATA CCAACATCCG GATGCCCATC TACAATTCGC ACGGCAAGAT GAAGAACTAC 
AAGTGCAAGA CCTGCGGCGT GGTGGCCATC ACCAAGGTGG ACTTCTGGGC GCACACCCGC 
ACCCACATGA AACCAGACAA GATCCTGCAG TGCCCGAAGT GCCCGTTCGT CACCGAGTTC 
AAGCACCACT TGGAGTACCA TATCCGGAAG CACAAGAACC AAAAGCCCTT CCAGTGCGAC 
AAATGCAGCT ACACGTGTGT CAACAAATCC ATGCTAAACT CGCACCGCAA GTCGCACAGT 
TCTGTGTATC AGTACCGTTG TGCGGATTGT GATTACGCCA CCAAGTATTG CCACAGCTTC 
AAGCTGCATC TGCGCAAGTA TGGTCACAAG CCCGGCATGG TTTTGGACGA GGATGGCACC 
CCGAATCCCT CGTTGGTCAT CGATGTTTAC GGCACGCGTC GTGGTCCGAA GAGCAAGAAT 
GGTGGACCGA TTGCCAGTGG AGGAAGTGGC AGCGGCAGCC GGAAGTCAAA TGTTGCAGCT 
GTCGCTCCGC AGCAACAGCA ATCTCAGCCA GCTCAGCCAG TCGCCACATC TCAGCTGAGT 
GCCGCCCTGC AAGGATTCCC TCTGGTTCAA GGCAACTCCG CTCCTCCGGC GGCATCTCCA 
GTGCTCCCGC TGCCCGCCTC TCCTGCCAAG AGTGTGGCCA GTGTGGAACA GACGCCCAGC 
TTGCCCAGTC CAGCCAATCT TCTGCCTCCT CTGGCCAGCC TTCTGCAGCA GAACCGCAAC 
ATGGCCTTCT TCCCCTACTG GAACCTCAAT CTCCAGATGC TGGCCGCCCA ACAACAGGCC 
GCTGTCTTGG CCCAATTGTC GCCAAGAATG CGAGAGCAAC TGCAGCAACA GAACCAGCAG 
CAGAGCGACA ATGAGGAGGA GGAGCAGGAC GATGAGTACG AGCGTAAGTC AGTGGACTCT 
GCCATGGATC TGTCCCAAGG AACGCCAGTG AAGGAGGATG AGCAGCAGCA ACAACCGCAG 
CAGCCGCTGG CCATGAATCT CAAGGTGGAG GAGGAGGCCA CGCCTCTGAT GAGCAGCTCG 
AATGCCTCGA GACGCAAGGG ACGCGTCCTC AAGCTGGACA CCCTGTTACA ACTGCGATCG 
GAGGCCATGA CATCTCCCGA GCAACTGAAA GTACCCAGCA CACCCATGCC AACTGCATCC 
TCGCCCATTG CCGGACGCAA ACCCATGCCC GAGGAGCACT GCTCGGGCAC CAGTTCGGCA 
GATGAGTCGA TGGAGACGGC CCATGTGCCG CAGGCCAATA CCAGTGCCAG TTCGACGGCG 
TCCAGCTCGG GGAACAGCTC CAATGCCAGC AGCAATAGCA ACGGCAACAG CAGCAGCAAT 
TCCAGCAGCA ATGGAACCAC CTCAGCGGTT GCAGCTCCTC CATCCGGAAC TCCGGCGGCG 
GCGGGTGCCA TCTACGAGTG CAAGTACTGT GATATCTTCT TCAAGGACGC CGTGCTCTAC 
ACCATTCACA TGGGCTACCA CAGCTGCGAC GATGTGTTCA AGTGCAACAT GTGCGGCGAG 
AAGTGCGACG GACCCGTCGG CCTCTTCGTT CACATGGCCA GGAATGCTCA CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731268.1
CDS	516..2792
Translation	MQNWETTATTNYEQHNAWYNSMFAANIKQEPGHHLDGNSVASSPRQSPIPSTNHLEQFLKQQQQQLQQQPMDTLCAMTPSPSQNDQNSLQHYDANLQQQLLQQQQYQQHFQAAQQQHHHHHHLMGGFNPLTPPGLPNPMQHFYGGNLRPSPQPTPTSASTIAPVAVATGSSEKLQALTPPMDVTPPKSPAKSSQSNIEPEKEHDQMSNSSEDMKYMAESEDDDTNIRMPIYNSHGKMKNYKCKTCGVVAITKVDFWAHTRTHMKPDKILQCPKCPFVTEFKHHLEYHIRKHKNQKPFQCDKCSYTCVNKSMLNSHRKSHSSVYQYRCADCDYATKYCHSFKLHLRKYGHKPGMVLDEDGTPNPSLVIDVYGTRRGPKSKNGGPIASGGSGSGSRKSNVAAVAPQQQQSQPAQPVATSQLSAALQGFPLVQGNSAPPAASPVLPLPASPAKSVASVEQTPSLPSPANLLPPLASLLQQNRNMAFFPYWNLNLQMLAAQQQAAVLAQLSPRMREQLQQQNQQQSDNEEEEQDDEYERKSVDSAMDLSQGTPVKEDEQQQQPQQPLAMNLKVEEEATPLMSSSNASRRKGRVLKLDTLLQLRSEAMTSPEQLKVPSTPMPTASSPIAGRKPMPEEHCSGTSSADESMETAHVPQANTSASSTASSSGNSSNASSNSNGNSSSNSSSNGTTSAVAAPPSGTPAAAGAIYECKYCDIFFKDAVLYTIHMGYHSCDDVFKCNMCGEKCDGPVGLFVHMARNAHS

Target ORF information:

RefSeq Version	NM_169234.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hunchback (hb), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169234.2

ORF Insert Sequence:

ATGCAGAACT GGGAGACGAC AGCCACGACC AACTACGAGC AGCACAACGC CTGGTACAAC 
AGCATGTTCG CGGCAAATAT CAAACAGGAG CCAGGTCATC ATCTCGACGG GAATAGCGTG 
GCCAGCAGTC CGCGCCAATC GCCCATTCCC TCGACCAATC ACCTGGAACA GTTCCTCAAG 
CAGCAGCAGC AGCAGCTTCA GCAGCAACCC ATGGATACCC TGTGCGCCAT GACCCCATCA 
CCCAGCCAAA ACGATCAAAA CAGCCTGCAG CATTACGATG CTAACTTGCA GCAACAGTTG 
CTGCAGCAAC AGCAGTACCA GCAGCATTTC CAGGCAGCCC AGCAGCAACA TCATCACCAT 
CACCATCTGA TGGGTGGATT CAATCCGCTG ACGCCACCTG GTCTGCCCAA TCCCATGCAG 
CACTTCTATG GCGGCAATCT GCGACCCAGT CCGCAGCCCA CGCCCACATC TGCCTCCACA 
ATTGCGCCCG TTGCAGTTGC CACTGGCAGC AGCGAGAAGT TGCAGGCACT AACACCACCC 
ATGGATGTCA CACCGCCTAA GTCGCCGGCC AAGTCGAGTC AGTCGAATAT TGAGCCGGAG 
AAGGAGCACG ATCAGATGTC GAACTCCAGC GAGGACATGA AGTACATGGC CGAGTCCGAG 
GACGATGATA CCAACATCCG GATGCCCATC TACAATTCGC ACGGCAAGAT GAAGAACTAC 
AAGTGCAAGA CCTGCGGCGT GGTGGCCATC ACCAAGGTGG ACTTCTGGGC GCACACCCGC 
ACCCACATGA AACCAGACAA GATCCTGCAG TGCCCGAAGT GCCCGTTCGT CACCGAGTTC 
AAGCACCACT TGGAGTACCA TATCCGGAAG CACAAGAACC AAAAGCCCTT CCAGTGCGAC 
AAATGCAGCT ACACGTGTGT CAACAAATCC ATGCTAAACT CGCACCGCAA GTCGCACAGT 
TCTGTGTATC AGTACCGTTG TGCGGATTGT GATTACGCCA CCAAGTATTG CCACAGCTTC 
AAGCTGCATC TGCGCAAGTA TGGTCACAAG CCCGGCATGG TTTTGGACGA GGATGGCACC 
CCGAATCCCT CGTTGGTCAT CGATGTTTAC GGCACGCGTC GTGGTCCGAA GAGCAAGAAT 
GGTGGACCGA TTGCCAGTGG AGGAAGTGGC AGCGGCAGCC GGAAGTCAAA TGTTGCAGCT 
GTCGCTCCGC AGCAACAGCA ATCTCAGCCA GCTCAGCCAG TCGCCACATC TCAGCTGAGT 
GCCGCCCTGC AAGGATTCCC TCTGGTTCAA GGCAACTCCG CTCCTCCGGC GGCATCTCCA 
GTGCTCCCGC TGCCCGCCTC TCCTGCCAAG AGTGTGGCCA GTGTGGAACA GACGCCCAGC 
TTGCCCAGTC CAGCCAATCT TCTGCCTCCT CTGGCCAGCC TTCTGCAGCA GAACCGCAAC 
ATGGCCTTCT TCCCCTACTG GAACCTCAAT CTCCAGATGC TGGCCGCCCA ACAACAGGCC 
GCTGTCTTGG CCCAATTGTC GCCAAGAATG CGAGAGCAAC TGCAGCAACA GAACCAGCAG 
CAGAGCGACA ATGAGGAGGA GGAGCAGGAC GATGAGTACG AGCGTAAGTC AGTGGACTCT 
GCCATGGATC TGTCCCAAGG AACGCCAGTG AAGGAGGATG AGCAGCAGCA ACAACCGCAG 
CAGCCGCTGG CCATGAATCT CAAGGTGGAG GAGGAGGCCA CGCCTCTGAT GAGCAGCTCG 
AATGCCTCGA GACGCAAGGG ACGCGTCCTC AAGCTGGACA CCCTGTTACA ACTGCGATCG 
GAGGCCATGA CATCTCCCGA GCAACTGAAA GTACCCAGCA CACCCATGCC AACTGCATCC 
TCGCCCATTG CCGGACGCAA ACCCATGCCC GAGGAGCACT GCTCGGGCAC CAGTTCGGCA 
GATGAGTCGA TGGAGACGGC CCATGTGCCG CAGGCCAATA CCAGTGCCAG TTCGACGGCG 
TCCAGCTCGG GGAACAGCTC CAATGCCAGC AGCAATAGCA ACGGCAACAG CAGCAGCAAT 
TCCAGCAGCA ATGGAACCAC CTCAGCGGTT GCAGCTCCTC CATCCGGAAC TCCGGCGGCG 
GCGGGTGCCA TCTACGAGTG CAAGTACTGT GATATCTTCT TCAAGGACGC CGTGCTCTAC 
ACCATTCACA TGGGCTACCA CAGCTGCGAC GATGTGTTCA AGTGCAACAT GTGCGGCGAG 
AAGTGCGACG GACCCGTCGG CCTCTTCGTT CACATGGCCA GGAATGCTCA CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hb ( NM_169233.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731267.1 Drosophila melanogaster hunchback (hb), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04254
Clone ID Related Accession (Same CDS sequence)	NM_169234.2 , NM_169233.2
Accession Version	NM_169233.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hunchback, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169233.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAACT GGGAGACGAC AGCCACGACC AACTACGAGC AGCACAACGC CTGGTACAAC 
AGCATGTTCG CGGCAAATAT CAAACAGGAG CCAGGTCATC ATCTCGACGG GAATAGCGTG 
GCCAGCAGTC CGCGCCAATC GCCCATTCCC TCGACCAATC ACCTGGAACA GTTCCTCAAG 
CAGCAGCAGC AGCAGCTTCA GCAGCAACCC ATGGATACCC TGTGCGCCAT GACCCCATCA 
CCCAGCCAAA ACGATCAAAA CAGCCTGCAG CATTACGATG CTAACTTGCA GCAACAGTTG 
CTGCAGCAAC AGCAGTACCA GCAGCATTTC CAGGCAGCCC AGCAGCAACA TCATCACCAT 
CACCATCTGA TGGGTGGATT CAATCCGCTG ACGCCACCTG GTCTGCCCAA TCCCATGCAG 
CACTTCTATG GCGGCAATCT GCGACCCAGT CCGCAGCCCA CGCCCACATC TGCCTCCACA 
ATTGCGCCCG TTGCAGTTGC CACTGGCAGC AGCGAGAAGT TGCAGGCACT AACACCACCC 
ATGGATGTCA CACCGCCTAA GTCGCCGGCC AAGTCGAGTC AGTCGAATAT TGAGCCGGAG 
AAGGAGCACG ATCAGATGTC GAACTCCAGC GAGGACATGA AGTACATGGC CGAGTCCGAG 
GACGATGATA CCAACATCCG GATGCCCATC TACAATTCGC ACGGCAAGAT GAAGAACTAC 
AAGTGCAAGA CCTGCGGCGT GGTGGCCATC ACCAAGGTGG ACTTCTGGGC GCACACCCGC 
ACCCACATGA AACCAGACAA GATCCTGCAG TGCCCGAAGT GCCCGTTCGT CACCGAGTTC 
AAGCACCACT TGGAGTACCA TATCCGGAAG CACAAGAACC AAAAGCCCTT CCAGTGCGAC 
AAATGCAGCT ACACGTGTGT CAACAAATCC ATGCTAAACT CGCACCGCAA GTCGCACAGT 
TCTGTGTATC AGTACCGTTG TGCGGATTGT GATTACGCCA CCAAGTATTG CCACAGCTTC 
AAGCTGCATC TGCGCAAGTA TGGTCACAAG CCCGGCATGG TTTTGGACGA GGATGGCACC 
CCGAATCCCT CGTTGGTCAT CGATGTTTAC GGCACGCGTC GTGGTCCGAA GAGCAAGAAT 
GGTGGACCGA TTGCCAGTGG AGGAAGTGGC AGCGGCAGCC GGAAGTCAAA TGTTGCAGCT 
GTCGCTCCGC AGCAACAGCA ATCTCAGCCA GCTCAGCCAG TCGCCACATC TCAGCTGAGT 
GCCGCCCTGC AAGGATTCCC TCTGGTTCAA GGCAACTCCG CTCCTCCGGC GGCATCTCCA 
GTGCTCCCGC TGCCCGCCTC TCCTGCCAAG AGTGTGGCCA GTGTGGAACA GACGCCCAGC 
TTGCCCAGTC CAGCCAATCT TCTGCCTCCT CTGGCCAGCC TTCTGCAGCA GAACCGCAAC 
ATGGCCTTCT TCCCCTACTG GAACCTCAAT CTCCAGATGC TGGCCGCCCA ACAACAGGCC 
GCTGTCTTGG CCCAATTGTC GCCAAGAATG CGAGAGCAAC TGCAGCAACA GAACCAGCAG 
CAGAGCGACA ATGAGGAGGA GGAGCAGGAC GATGAGTACG AGCGTAAGTC AGTGGACTCT 
GCCATGGATC TGTCCCAAGG AACGCCAGTG AAGGAGGATG AGCAGCAGCA ACAACCGCAG 
CAGCCGCTGG CCATGAATCT CAAGGTGGAG GAGGAGGCCA CGCCTCTGAT GAGCAGCTCG 
AATGCCTCGA GACGCAAGGG ACGCGTCCTC AAGCTGGACA CCCTGTTACA ACTGCGATCG 
GAGGCCATGA CATCTCCCGA GCAACTGAAA GTACCCAGCA CACCCATGCC AACTGCATCC 
TCGCCCATTG CCGGACGCAA ACCCATGCCC GAGGAGCACT GCTCGGGCAC CAGTTCGGCA 
GATGAGTCGA TGGAGACGGC CCATGTGCCG CAGGCCAATA CCAGTGCCAG TTCGACGGCG 
TCCAGCTCGG GGAACAGCTC CAATGCCAGC AGCAATAGCA ACGGCAACAG CAGCAGCAAT 
TCCAGCAGCA ATGGAACCAC CTCAGCGGTT GCAGCTCCTC CATCCGGAAC TCCGGCGGCG 
GCGGGTGCCA TCTACGAGTG CAAGTACTGT GATATCTTCT TCAAGGACGC CGTGCTCTAC 
ACCATTCACA TGGGCTACCA CAGCTGCGAC GATGTGTTCA AGTGCAACAT GTGCGGCGAG 
AAGTGCGACG GACCCGTCGG CCTCTTCGTT CACATGGCCA GGAATGCTCA CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731267.1
CDS	164..2440
Translation	MQNWETTATTNYEQHNAWYNSMFAANIKQEPGHHLDGNSVASSPRQSPIPSTNHLEQFLKQQQQQLQQQPMDTLCAMTPSPSQNDQNSLQHYDANLQQQLLQQQQYQQHFQAAQQQHHHHHHLMGGFNPLTPPGLPNPMQHFYGGNLRPSPQPTPTSASTIAPVAVATGSSEKLQALTPPMDVTPPKSPAKSSQSNIEPEKEHDQMSNSSEDMKYMAESEDDDTNIRMPIYNSHGKMKNYKCKTCGVVAITKVDFWAHTRTHMKPDKILQCPKCPFVTEFKHHLEYHIRKHKNQKPFQCDKCSYTCVNKSMLNSHRKSHSSVYQYRCADCDYATKYCHSFKLHLRKYGHKPGMVLDEDGTPNPSLVIDVYGTRRGPKSKNGGPIASGGSGSGSRKSNVAAVAPQQQQSQPAQPVATSQLSAALQGFPLVQGNSAPPAASPVLPLPASPAKSVASVEQTPSLPSPANLLPPLASLLQQNRNMAFFPYWNLNLQMLAAQQQAAVLAQLSPRMREQLQQQNQQQSDNEEEEQDDEYERKSVDSAMDLSQGTPVKEDEQQQQPQQPLAMNLKVEEEATPLMSSSNASRRKGRVLKLDTLLQLRSEAMTSPEQLKVPSTPMPTASSPIAGRKPMPEEHCSGTSSADESMETAHVPQANTSASSTASSSGNSSNASSNSNGNSSSNSSSNGTTSAVAAPPSGTPAAAGAIYECKYCDIFFKDAVLYTIHMGYHSCDDVFKCNMCGEKCDGPVGLFVHMARNAHS

Target ORF information:

RefSeq Version	NM_169233.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hunchback (hb), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169233.2

ORF Insert Sequence:

ATGCAGAACT GGGAGACGAC AGCCACGACC AACTACGAGC AGCACAACGC CTGGTACAAC 
AGCATGTTCG CGGCAAATAT CAAACAGGAG CCAGGTCATC ATCTCGACGG GAATAGCGTG 
GCCAGCAGTC CGCGCCAATC GCCCATTCCC TCGACCAATC ACCTGGAACA GTTCCTCAAG 
CAGCAGCAGC AGCAGCTTCA GCAGCAACCC ATGGATACCC TGTGCGCCAT GACCCCATCA 
CCCAGCCAAA ACGATCAAAA CAGCCTGCAG CATTACGATG CTAACTTGCA GCAACAGTTG 
CTGCAGCAAC AGCAGTACCA GCAGCATTTC CAGGCAGCCC AGCAGCAACA TCATCACCAT 
CACCATCTGA TGGGTGGATT CAATCCGCTG ACGCCACCTG GTCTGCCCAA TCCCATGCAG 
CACTTCTATG GCGGCAATCT GCGACCCAGT CCGCAGCCCA CGCCCACATC TGCCTCCACA 
ATTGCGCCCG TTGCAGTTGC CACTGGCAGC AGCGAGAAGT TGCAGGCACT AACACCACCC 
ATGGATGTCA CACCGCCTAA GTCGCCGGCC AAGTCGAGTC AGTCGAATAT TGAGCCGGAG 
AAGGAGCACG ATCAGATGTC GAACTCCAGC GAGGACATGA AGTACATGGC CGAGTCCGAG 
GACGATGATA CCAACATCCG GATGCCCATC TACAATTCGC ACGGCAAGAT GAAGAACTAC 
AAGTGCAAGA CCTGCGGCGT GGTGGCCATC ACCAAGGTGG ACTTCTGGGC GCACACCCGC 
ACCCACATGA AACCAGACAA GATCCTGCAG TGCCCGAAGT GCCCGTTCGT CACCGAGTTC 
AAGCACCACT TGGAGTACCA TATCCGGAAG CACAAGAACC AAAAGCCCTT CCAGTGCGAC 
AAATGCAGCT ACACGTGTGT CAACAAATCC ATGCTAAACT CGCACCGCAA GTCGCACAGT 
TCTGTGTATC AGTACCGTTG TGCGGATTGT GATTACGCCA CCAAGTATTG CCACAGCTTC 
AAGCTGCATC TGCGCAAGTA TGGTCACAAG CCCGGCATGG TTTTGGACGA GGATGGCACC 
CCGAATCCCT CGTTGGTCAT CGATGTTTAC GGCACGCGTC GTGGTCCGAA GAGCAAGAAT 
GGTGGACCGA TTGCCAGTGG AGGAAGTGGC AGCGGCAGCC GGAAGTCAAA TGTTGCAGCT 
GTCGCTCCGC AGCAACAGCA ATCTCAGCCA GCTCAGCCAG TCGCCACATC TCAGCTGAGT 
GCCGCCCTGC AAGGATTCCC TCTGGTTCAA GGCAACTCCG CTCCTCCGGC GGCATCTCCA 
GTGCTCCCGC TGCCCGCCTC TCCTGCCAAG AGTGTGGCCA GTGTGGAACA GACGCCCAGC 
TTGCCCAGTC CAGCCAATCT TCTGCCTCCT CTGGCCAGCC TTCTGCAGCA GAACCGCAAC 
ATGGCCTTCT TCCCCTACTG GAACCTCAAT CTCCAGATGC TGGCCGCCCA ACAACAGGCC 
GCTGTCTTGG CCCAATTGTC GCCAAGAATG CGAGAGCAAC TGCAGCAACA GAACCAGCAG 
CAGAGCGACA ATGAGGAGGA GGAGCAGGAC GATGAGTACG AGCGTAAGTC AGTGGACTCT 
GCCATGGATC TGTCCCAAGG AACGCCAGTG AAGGAGGATG AGCAGCAGCA ACAACCGCAG 
CAGCCGCTGG CCATGAATCT CAAGGTGGAG GAGGAGGCCA CGCCTCTGAT GAGCAGCTCG 
AATGCCTCGA GACGCAAGGG ACGCGTCCTC AAGCTGGACA CCCTGTTACA ACTGCGATCG 
GAGGCCATGA CATCTCCCGA GCAACTGAAA GTACCCAGCA CACCCATGCC AACTGCATCC 
TCGCCCATTG CCGGACGCAA ACCCATGCCC GAGGAGCACT GCTCGGGCAC CAGTTCGGCA 
GATGAGTCGA TGGAGACGGC CCATGTGCCG CAGGCCAATA CCAGTGCCAG TTCGACGGCG 
TCCAGCTCGG GGAACAGCTC CAATGCCAGC AGCAATAGCA ACGGCAACAG CAGCAGCAAT 
TCCAGCAGCA ATGGAACCAC CTCAGCGGTT GCAGCTCCTC CATCCGGAAC TCCGGCGGCG 
GCGGGTGCCA TCTACGAGTG CAAGTACTGT GATATCTTCT TCAAGGACGC CGTGCTCTAC 
ACCATTCACA TGGGCTACCA CAGCTGCGAC GATGTGTTCA AGTGCAACAT GTGCGGCGAG 
AAGTGCGACG GACCCGTCGG CCTCTTCGTT CACATGGCCA GGAATGCTCA CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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hb cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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