ato cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ato
Entrez Gene ID	40975
Full Name	atonal
Synonyms	ATO,At,Ato,CG7508,Dmel\CG7508,ato1,ato[Dm],bHLHa10
General protein information	Preferred Names atonal Names CG7508 gene product from transcript CG7508-RA CG7508-PA ato-PA atona
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84F6-84F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_169213.2	NP_731223.1	atonal

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	ato	NP_731223.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001741	XP_321345.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the regulation of ato expression in the Bolwig's organs primordium, was examined.
Title: Distinct regulation of atonal in a visual organ of Drosophila: Organ-specific enhancer and lack of autoregulation in the larval eye.

three binding sites are essential for atonal induction in the posterior ocelli, but show considerable redundancy in the anterior ocellus. this difference parallels the distinct control of ato transcription in the posterior and anterior progenitors of the developing compound eyes.
Title: Shared and distinct mechanisms of atonal regulation in Drosophila ocelli and compound eyes.

genetic ablation of Atonal Olfactory receptor neurons (ORNs) affects antennal lobe structure and axon targeting of Amos ORNs.
Title: Hierarchical axon targeting of Drosophila olfactory receptor neurons specified by the proneural transcription factors Atonal and Amos.

Data show that eyes absent protein (Eya) is required in late progenitors to promote eye formation, and that failure to induce the target gene atonal and consequent lack of neuron formation.
Title: ato-Gal4 fly lines for gene function analysis: Eya is required in late progenitors for eye morphogenesis.

atonal expression in Drosophila retinal progenitors involves redundant and synergistic contributions of Ey/Pax6 and So binding sites within two distant enhancers
Title: Onset of atonal expression in Drosophila retinal progenitors involves redundant and synergistic contributions of Ey/Pax6 and So binding sites within two distant enhancers.

The following ato gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ato cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08117	NM_169213.2 Latest version!	Drosophila melanogaster atonal (ato), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ato ( NM_169213.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731223.1 Drosophila melanogaster atonal (ato), mRNA.

Price & Availability↑

CloneID	OFa08117
Clone ID Related Accession (Same CDS sequence)	NM_169213.2
Accession Version	NM_169213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	atonal
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169213.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCCA GTGAGATCTA TCGCTACTAC TACAAGACCT CCGAGGACTT GCAGGGCTTC 
AAGACAGCCG CCGCCGAGCC GTACTTCAAT CCCATGGCAG CCTACAATCC CGGCGTGACC 
CACTACCAGT TCAATGGCAA CACCCTGGCC AGCAGCAGCA ACTACTTGTC GGCCAATGGC 
TTCATCAGCT TCGAGCAGGC CAGTTCCGAT GGCTGGATCT CCTCCTCGCC GGCTAGCCAC 
CGATCTGAGA GTCCCGAGTA TGTGGATCTC AATACCATGT ACAATGGAGG CTGCAACAAC 
ATGGCCCAGA ACCAACAATA CGGAATGATT ATGGAGCAGT CTGTTGTTTC CACAGCGCCT 
GCAATTCCAG TGGCCTCTCC TCCGGCAGTG GAGGTCATGG GCTCCTCCAA CGTGGGCACT 
TGCAAAACGA TTCCAGCCTC AGCAGGTCCG AAACCGAAGC GTAGCTATAC CAAGAAGAAC 
CAGCCAAGCA CCACCGCCAC CTCCACACCG ACTGCAGCTG CGGAGTCATC TGCCTCAGTG 
AATCTCTACA CGGAGGAGTT CCAGAACTTT GACTTTGACA ACTCCGCCTT GTTCGATGAC 
AGCGTCGAGG ATGACGAGGA CCTCATGCTC TTCAGTGGCG GTGAGGACTT CGATGGCAAT 
GATGGATCCT TTGACTTGGC CGATGGTGAG AACCAAGATG CCGCTGCCGG AGGCTCTGGA 
AAGAAGAGGC GTGGCAAGCA GATCACACCC GTCGTGAAGA GGAAGCGTCG CCTGGCCGCC 
AATGCACGTG AGCGTCGTCG GATGCAGAAC CTCAACCAGG CCTTCGATCG TCTCCGCCAG 
TACCTTCCCT GTCTGGGAAA CGATCGCCAG CTGTCCAAAC ACGAGACCCT CCAAATGGCC 
CAGACCTACA TATCCGCTCT CGGGGATCTG CTGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731223.1
CDS	75..1013
Translation	MSSSEIYRYYYKTSEDLQGFKTAAAEPYFNPMAAYNPGVTHYQFNGNTLASSSNYLSANGFISFEQASSDGWISSSPASHRSESPEYVDLNTMYNGGCNNMAQNQQYGMIMEQSVVSTAPAIPVASPPAVEVMGSSNVGTCKTIPASAGPKPKRSYTKKNQPSTTATSTPTAAAESSASVNLYTEEFQNFDFDNSALFDDSVEDDEDLMLFSGGEDFDGNDGSFDLADGENQDAAAGGSGKKRRGKQITPVVKRKRRLAANARERRRMQNLNQAFDRLRQYLPCLGNDRQLSKHETLQMAQTYISALGDLLR

Target ORF information:

RefSeq Version	NM_169213.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster atonal (ato), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169213.2

ORF Insert Sequence:

ATGTCGTCCA GTGAGATCTA TCGCTACTAC TACAAGACCT CCGAGGACTT GCAGGGCTTC 
AAGACAGCCG CCGCCGAGCC GTACTTCAAT CCCATGGCAG CCTACAATCC CGGCGTGACC 
CACTACCAGT TCAATGGCAA CACCCTGGCC AGCAGCAGCA ACTACTTGTC GGCCAATGGC 
TTCATCAGCT TCGAGCAGGC CAGTTCCGAT GGCTGGATCT CCTCCTCGCC GGCTAGCCAC 
CGATCTGAGA GTCCCGAGTA TGTGGATCTC AATACCATGT ACAATGGAGG CTGCAACAAC 
ATGGCCCAGA ACCAACAATA CGGAATGATT ATGGAGCAGT CTGTTGTTTC CACAGCGCCT 
GCAATTCCAG TGGCCTCTCC TCCGGCAGTG GAGGTCATGG GCTCCTCCAA CGTGGGCACT 
TGCAAAACGA TTCCAGCCTC AGCAGGTCCG AAACCGAAGC GTAGCTATAC CAAGAAGAAC 
CAGCCAAGCA CCACCGCCAC CTCCACACCG ACTGCAGCTG CGGAGTCATC TGCCTCAGTG 
AATCTCTACA CGGAGGAGTT CCAGAACTTT GACTTTGACA ACTCCGCCTT GTTCGATGAC 
AGCGTCGAGG ATGACGAGGA CCTCATGCTC TTCAGTGGCG GTGAGGACTT CGATGGCAAT 
GATGGATCCT TTGACTTGGC CGATGGTGAG AACCAAGATG CCGCTGCCGG AGGCTCTGGA 
AAGAAGAGGC GTGGCAAGCA GATCACACCC GTCGTGAAGA GGAAGCGTCG CCTGGCCGCC 
AATGCACGTG AGCGTCGTCG GATGCAGAAC CTCAACCAGG CCTTCGATCG TCTCCGCCAG 
TACCTTCCCT GTCTGGGAAA CGATCGCCAG CTGTCCAAAC ACGAGACCCT CCAAATGGCC 
CAGACCTACA TATCCGCTCT CGGGGATCTG CTGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ato cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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