mAChR-B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mAChR-B
Entrez Gene ID	40955
Full Name	muscarinic Acetylcholine Receptor, B-type
Synonyms	CG7918,Dm-mAChR-B,Dmel\CG7918,anon-WO0170980.91,anon-WO0170980.92,mAChR B,mAcR-CG7918
General protein information	Preferred Names muscarinic Acetylcholine Receptor, B-type Names CG7918 gene product from transcript CG7918-RA B-type mAChR CG7918-PA CG7918-PB mAChR-B-PA mAChR-B-PB muscarinic acetylcholine Receptor, B-type
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84E10-84E10

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300292.1	NP_001287221.1	muscarinic acetylcholine Receptor, B-type, isoform B
NM_141507.2	NP_649764.1	muscarinic acetylcholine Receptor, B-type, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG7918	NP_649764.1
Anopheles gambiae (African malaria mosquito)	GPRMAC2	XP_313548.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mAChR-B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mAChR-B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa22063	NM_001300292.1 Latest version!	Drosophila melanogaster muscarinic acetylcholine Receptor, B-type, transcript variant B (mAChR-B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa08121	NM_141507.2 Latest version!	Drosophila melanogaster muscarinic acetylcholine Receptor, B-type, transcript variant A (mAChR-B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mAChR-B ( NM_001300292.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287221.1 Drosophila melanogaster muscarinic acetylcholine Receptor, B-type, transcript variant B (mAChR-B), mRNA.

Price & Availability↑

CloneID	OFa22063
Clone ID Related Accession (Same CDS sequence)	NM_001300292.1
Accession Version	NM_001300292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	muscarinic acetylcholine Receptor, B-type, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGTCGC TTAACAAGTC GGCGGGCGAG GAGGAGCTGA CTGGCCTGAA TGGCAACTCC 
AGCGACTCCA TCTATACCAT CAAGAACAAG TGGATCACCG ACTGGAGCCT TAGAAGGGAG 
CGGGATAAGC TGTGCAGCTT CATTTTCAAG CGAGTCCTGA CGCACACGGA GTGCAAACGT 
CTGTTGGAGG CGGCCAAGAT CTATGGCGAG AGCAGTGACT TCAATTCCTG GCCGGTTAAT 
GAACAGCTGC AATGGTACAG GAACTTCGAT GTGAACAATA CGAACCTTCT GTTGGCTGTT 
AGAAATCAAT CCTCTGATGG CGGTTCCACC ATGTCGGGAT CCAGTTCCGA CTCGGAGATT 
CTTGGTCCTG TTTTGCCACC ATTTGCACTG TGGCAGACGA TCCTGATAGC CATATGCCTG 
GCGATCTGTA TCATTCTTAC GGTGGGCGGT AATATCCTGG TGCTACTGGC CTTCATCGTG 
GACAGGAATA TTCGGCAGCC CAGCAACTAC TTTATTGCCT CCCTAGCAGC CACGGATATG 
CTAATAGGAA CCGTTTCGAT GCCCTTCTAC ACGATATACG TGCTCAAGGG TTATTGGGAC 
CTGGGTCCGA TGCTCTGCGA TCTCTGGCTT TCCGTCGACT ATACTGTGTG TCTGGTGTCC 
CAGTACACGG TGCTGTTGAT CACGATAGAT CGCTACTGCT CTGTGAAGAT TGCCGCCAAG 
TACCGGAGTT GGAGGACGAG AACCAGAGTG ATATATATGG TCACAATCAC TTGGATCATT 
CCCGCTCTGC TCTTCTTCAT CTCGATTTTT GGATGGGAGC ACTTCACCGG CAAGCGGGAT 
CTACTTCCTG GCCAGTGCGC CGTTCAGTTT CTCAAGGATC CGATCTTTAA TACGGCCCTA 
ATCATTGGCT ACTATTGGAC CACACTAATT GTGCTTTTTG TCTTATATGC CGGGATCTAC 
AAAACAGCCT ATGATATGCA AAAGCGCAGT GAGGCGAAGC AACGGAAAAT GCAATCCATG 
GTGGCACTGA GTGCTGGAGC GATGTCCGGA ATGGCAGGTC ATGCAGCTGG CATTGGTGTG 
ATTGAGGAGA AAATCCTAAA GACCAAAGTG GAACTTGCGG GAGATCAAAC CGATTTGGAT 
AGTGCCTGCA CCACAGTGAT TAAGAGATTA AGTGGTTCCG GTCAGGCAAA TCCTCTGGCC 
GTGGCCACCG AGGAAGTGGA GAAGATGACA CCGGAACAGA GGAGAGCGTC GGCAGCAAAA 
ATCCAGGAGG AGGCGAAAAA ACGTGAGGCA GCCGTGGATG CGGAAAAGAG CGAGAGATCC 
AGCAGTCCTG CCTTCGATTC CGATGAGGAA TCATCCGTCA ACCAGGCCCA GCAGTTGATC 
ACCCAGCAGA AATTGAACAA CATGAGGAAG CGGTCATCGA TTGGTCTGGT CTTTGGGGCA 
CAGGCCGCCC TGCTGGCCAC TCGTGGCAAA GGAATCGAGG ACTTTGCTAA GGTGAGGCGG 
GAGAGCTGCA TTGAGGCCAT CTGCCTGTTG GATGTGCCCG GAAAGCTAGC TGCGGACTGT 
CCCCATCGCC GGGCCTCCAG TCCTATGGAG ACCAGCAGCA AGGATGCCAT ACTCTATTCC 
CCGATGCCAC CAATGCCACT CTCACCCAAG GTAAAGACTT CAACGCCACA AACGGGAACG 
CCCCAGTCGG CGGCGGGTGT AACACCACCG GTGCCACTGG AAAGGATTGA GGAGCGAGAA 
ACCTCGGACA GTAATACCAA CAAGATGCCC TCCACCTCGG GCACCACCAG CAGTACGGGC 
GGTGTGGGTG GAGGCGGGGA ACAAGACGGT GTTGGTGCCA CCAAAAAGAA CGCGGGGGAT 
GACGATGCCA ACAGGGAGGA GAAGAGTTTG GCGGCCAGCC GAAACTCCAG CAAAAGGGCA 
TTCATTCATT CGATAGGAAA GCACTTCAAG AGCAAGAAGG CTCTACCCCT GATCCTTGGA 
GTGGGTGGAC GGCAGAAGTC CAAGTCGGAG AATCGTGCTC GCAAAGCGTT TCGCACGATC 
TCGTTCATTT TGGGCTGCTT CGTGGCCTGC TGGACACCCT ACCATGTGTT GGCCCTCGTC 
GAGGGATTCT GCCGGCATCC GCCCTGCATC AATGAACACC TCTACATGTT CTCGTACTTC 
CTCTGCTACG CCAACAGCCC GATGAATCCT TTTTGCTACG CTCTGGCCAA TCAGCAGTTC 
AAGAAGACCT TTACGCGGAT CCTCAAGGGG GATCTGCACA TGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287221.1
CDS	446..2713
Translation	MRSLNKSAGEEELTGLNGNSSDSIYTIKNKWITDWSLRRERDKLCSFIFKRVLTHTECKRLLEAAKIYGESSDFNSWPVNEQLQWYRNFDVNNTNLLLAVRNQSSDGGSTMSGSSSDSEILGPVLPPFALWQTILIAICLAICIILTVGGNILVLLAFIVDRNIRQPSNYFIASLAATDMLIGTVSMPFYTIYVLKGYWDLGPMLCDLWLSVDYTVCLVSQYTVLLITIDRYCSVKIAAKYRSWRTRTRVIYMVTITWIIPALLFFISIFGWEHFTGKRDLLPGQCAVQFLKDPIFNTALIIGYYWTTLIVLFVLYAGIYKTAYDMQKRSEAKQRKMQSMVALSAGAMSGMAGHAAGIGVIEEKILKTKVELAGDQTDLDSACTTVIKRLSGSGQANPLAVATEEVEKMTPEQRRASAAKIQEEAKKREAAVDAEKSERSSSPAFDSDEESSVNQAQQLITQQKLNNMRKRSSIGLVFGAQAALLATRGKGIEDFAKVRRESCIEAICLLDVPGKLAADCPHRRASSPMETSSKDAILYSPMPPMPLSPKVKTSTPQTGTPQSAAGVTPPVPLERIEERETSDSNTNKMPSTSGTTSSTGGVGGGGEQDGVGATKKNAGDDDANREEKSLAASRNSSKRAFIHSIGKHFKSKKALPLILGVGGRQKSKSENRARKAFRTISFILGCFVACWTPYHVLALVEGFCRHPPCINEHLYMFSYFLCYANSPMNPFCYALANQQFKKTFTRILKGDLHMT

Target ORF information:

RefSeq Version	NM_001300292.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster muscarinic acetylcholine Receptor, B-type, transcript variant B (mAChR-B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300292.1

ORF Insert Sequence:

ATGCGGTCGC TTAACAAGTC GGCGGGCGAG GAGGAGCTGA CTGGCCTGAA TGGCAACTCC 
AGCGACTCCA TCTATACCAT CAAGAACAAG TGGATCACCG ACTGGAGCCT TAGAAGGGAG 
CGGGATAAGC TGTGCAGCTT CATTTTCAAG CGAGTCCTGA CGCACACGGA GTGCAAACGT 
CTGTTGGAGG CGGCCAAGAT CTATGGCGAG AGCAGTGACT TCAATTCCTG GCCGGTTAAT 
GAACAGCTGC AATGGTACAG GAACTTCGAT GTGAACAATA CGAACCTTCT GTTGGCTGTT 
AGAAATCAAT CCTCTGATGG CGGTTCCACC ATGTCGGGAT CCAGTTCCGA CTCGGAGATT 
CTTGGTCCTG TTTTGCCACC ATTTGCACTG TGGCAGACGA TCCTGATAGC CATATGCCTG 
GCGATCTGTA TCATTCTTAC GGTGGGCGGT AATATCCTGG TGCTACTGGC CTTCATCGTG 
GACAGGAATA TTCGGCAGCC CAGCAACTAC TTTATTGCCT CCCTAGCAGC CACGGATATG 
CTAATAGGAA CCGTTTCGAT GCCCTTCTAC ACGATATACG TGCTCAAGGG TTATTGGGAC 
CTGGGTCCGA TGCTCTGCGA TCTCTGGCTT TCCGTCGACT ATACTGTGTG TCTGGTGTCC 
CAGTACACGG TGCTGTTGAT CACGATAGAT CGCTACTGCT CTGTGAAGAT TGCCGCCAAG 
TACCGGAGTT GGAGGACGAG AACCAGAGTG ATATATATGG TCACAATCAC TTGGATCATT 
CCCGCTCTGC TCTTCTTCAT CTCGATTTTT GGATGGGAGC ACTTCACCGG CAAGCGGGAT 
CTACTTCCTG GCCAGTGCGC CGTTCAGTTT CTCAAGGATC CGATCTTTAA TACGGCCCTA 
ATCATTGGCT ACTATTGGAC CACACTAATT GTGCTTTTTG TCTTATATGC CGGGATCTAC 
AAAACAGCCT ATGATATGCA AAAGCGCAGT GAGGCGAAGC AACGGAAAAT GCAATCCATG 
GTGGCACTGA GTGCTGGAGC GATGTCCGGA ATGGCAGGTC ATGCAGCTGG CATTGGTGTG 
ATTGAGGAGA AAATCCTAAA GACCAAAGTG GAACTTGCGG GAGATCAAAC CGATTTGGAT 
AGTGCCTGCA CCACAGTGAT TAAGAGATTA AGTGGTTCCG GTCAGGCAAA TCCTCTGGCC 
GTGGCCACCG AGGAAGTGGA GAAGATGACA CCGGAACAGA GGAGAGCGTC GGCAGCAAAA 
ATCCAGGAGG AGGCGAAAAA ACGTGAGGCA GCCGTGGATG CGGAAAAGAG CGAGAGATCC 
AGCAGTCCTG CCTTCGATTC CGATGAGGAA TCATCCGTCA ACCAGGCCCA GCAGTTGATC 
ACCCAGCAGA AATTGAACAA CATGAGGAAG CGGTCATCGA TTGGTCTGGT CTTTGGGGCA 
CAGGCCGCCC TGCTGGCCAC TCGTGGCAAA GGAATCGAGG ACTTTGCTAA GGTGAGGCGG 
GAGAGCTGCA TTGAGGCCAT CTGCCTGTTG GATGTGCCCG GAAAGCTAGC TGCGGACTGT 
CCCCATCGCC GGGCCTCCAG TCCTATGGAG ACCAGCAGCA AGGATGCCAT ACTCTATTCC 
CCGATGCCAC CAATGCCACT CTCACCCAAG GTAAAGACTT CAACGCCACA AACGGGAACG 
CCCCAGTCGG CGGCGGGTGT AACACCACCG GTGCCACTGG AAAGGATTGA GGAGCGAGAA 
ACCTCGGACA GTAATACCAA CAAGATGCCC TCCACCTCGG GCACCACCAG CAGTACGGGC 
GGTGTGGGTG GAGGCGGGGA ACAAGACGGT GTTGGTGCCA CCAAAAAGAA CGCGGGGGAT 
GACGATGCCA ACAGGGAGGA GAAGAGTTTG GCGGCCAGCC GAAACTCCAG CAAAAGGGCA 
TTCATTCATT CGATAGGAAA GCACTTCAAG AGCAAGAAGG CTCTACCCCT GATCCTTGGA 
GTGGGTGGAC GGCAGAAGTC CAAGTCGGAG AATCGTGCTC GCAAAGCGTT TCGCACGATC 
TCGTTCATTT TGGGCTGCTT CGTGGCCTGC TGGACACCCT ACCATGTGTT GGCCCTCGTC 
GAGGGATTCT GCCGGCATCC GCCCTGCATC AATGAACACC TCTACATGTT CTCGTACTTC 
CTCTGCTACG CCAACAGCCC GATGAATCCT TTTTGCTACG CTCTGGCCAA TCAGCAGTTC 
AAGAAGACCT TTACGCGGAT CCTCAAGGGG GATCTGCACA TGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mAChR-B ( NM_141507.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649764.1 Drosophila melanogaster muscarinic acetylcholine Receptor, B-type, transcript variant A (mAChR-B), mRNA.

Price & Availability↑

CloneID	OFa08121
Clone ID Related Accession (Same CDS sequence)	NM_141507.2
Accession Version	NM_141507.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	muscarinic acetylcholine Receptor, B-type, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_141507.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGTCGC TTAACAAGTC GGCGGGCGAG GAGGAGCTGA CTGGCCTGAA TGGCAACTCC 
AGCGACTCCA TCTATACCAT CAAGAACAAG TGGATCACCG ACTGGAGCCT TAGAAGGGAG 
CGGGATAAGC TGTGCAGCTT CATTTTCAAG CGAGTCCTGA CGCACACGGA GTGCAAACGT 
CTGTTGGAGG CGGCCAAGAT CTATGGCGAG AGCAGTGACT TCAATTCCTG GCCGGTTAAT 
GAACAGCTGC AATGGTACAG GAACTTCGAT GTGAACAATA CGAACCTTCT GTTGGCTGTT 
AGAAATCAAT CCTCTGATGG CGGTTCCACC ATGTCGGGAT CCAGTTCCGA CTCGGAGATT 
CTTGGTCCTG TTTTGCCACC ATTTGCACTG TGGCAGACGA TCCTGATAGC CATATGCCTG 
GCGATCTGTA TCATTCTTAC GGTGGGCGGT AATATCCTGG TGCTACTGGC CTTCATCGTG 
GACAGGAATA TTCGGCAGCC CAGCAACTAC TTTATTGCCT CCCTAGCAGC CACGGATATG 
CTAATAGGAA CCGTTTCGAT GCCCTTCTAC ACGATATACG TGCTCAAGGG TTATTGGGAC 
CTGGGTCCGA TGCTCTGCGA TCTCTGGCTT TCCGTCGACT ATACTGTGTG TCTGGTGTCC 
CAGTACACGG TGCTGTTGAT CACGATAGAT CGCTACTGCT CTGTGAAGAT TGCCGCCAAG 
TACCGGAGTT GGAGGACGAG AACCAGAGTG ATATATATGG TCACAATCAC TTGGATCATT 
CCCGCTCTGC TCTTCTTCAT CTCGATTTTT GGATGGGAGC ACTTCACCGG CAAGCGGGAT 
CTACTTCCTG GCCAGTGCGC CGTTCAGTTT CTCAAGGATC CGATCTTTAA TACGGCCCTA 
ATCATTGGCT ACTATTGGAC CACACTAATT GTGCTTTTTG TCTTATATGC CGGGATCTAC 
AAAACAGCCT ATGATATGCA AAAGCGCAGT GAGGCGAAGC AACGGAAAAT GCAATCCATG 
GTGGCACTGA GTGCTGGAGC GATGTCCGGA ATGGCAGGTC ATGCAGCTGG CATTGGTGTG 
ATTGAGGAGA AAATCCTAAA GACCAAAGTG GAACTTGCGG GAGATCAAAC CGATTTGGAT 
AGTGCCTGCA CCACAGTGAT TAAGAGATTA AGTGGTTCCG GTCAGGCAAA TCCTCTGGCC 
GTGGCCACCG AGGAAGTGGA GAAGATGACA CCGGAACAGA GGAGAGCGTC GGCAGCAAAA 
ATCCAGGAGG AGGCGAAAAA ACGTGAGGCA GCCGTGGATG CGGAAAAGAG CGAGAGATCC 
AGCAGTCCTG CCTTCGATTC CGATGAGGAA TCATCCGTCA ACCAGGCCCA GCAGTTGATC 
ACCCAGCAGA AATTGAACAA CATGAGGAAG CGGTCATCGA TTGGTCTGGT CTTTGGGGCA 
CAGGCCGCCC TGCTGGCCAC TCGTGGCAAA GGTAACTTGC AGAAGAGCAC CACTAACTCC 
AAGTCGATCG AGGCCATGCA CCAGTACCAC CACCACCAAC AGCACCATCA CAACCAGAGT 
CCGTTACAGC GGGCGCAGAG CAAGGAAGAG ATGCGCTCCC TGCACCACCA CCAGAACCAG 
CAACAACCGC ACCACCAGCA CCACCAAAAC CAGCCGAACC CACCGCTGGA CAGGCCCAAG 
CGCACATCCT GCTCCACACT GTCCCAGATA GCCGAGCATG ATCGTTTGGT CGACCTGTCC 
GCTCCTCCAA CATTACTCAA CACCAGTGAT CCCCTGTCCC CCGTGGACTT GGCACCCATC 
GAGGTCCCTA GCGATCAAGT GCATCAGGGT CTGGTGCAGA CCATTTTGCC ACCTCCCGAC 
GCCTTTCAGT GCCCCACCCC GCTCTCCGAT GACTACTCGG ATCGTCCCTT TGGGAATAGT 
TCCGGTAACA GTGAACTGGC CCTGACCTAT GACCTGATGA CGAACTCGGA GCTACGCTAT 
ATGGACGAGA GCTCCGCCAT GCTGGCCAGT GTGACGGCCA ACTCGACGAC CTCCCCGAAT 
GACAGCGTCC AGGGGAAACC ACCAGTCCTG CCTCCACCAC CGCCCGCACG CCGAAATCCA 
CCAAAATCGA ACCTGAACCA GAGCCTCAGT CAAACTCCCA GCCAAAACCA GAGTCCCAGC 
CAAAGCCTGA GCCTCAACCT GAATCCGAAT CACAACCAGA GTCAGAGTCA GAGTATCGAG 
GAGGCGGTGG CCATTGAAAA ACGTCTTCTC GTCTCGTACA CAGGAATCGA GGACTTTGCT 
AAGGTGAGGC GGGAGAGCTG CATTGAGGCC ATCTGCCTGT TGGATGTGCC CGGAAAGCTA 
GCTGCGGACT GTCCCCATCG CCGGGCCTCC AGTCCTATGG AGACCAGCAG CAAGGATGCC 
ATACTCTATT CCCCGATGCC ACCAATGCCA CTCTCACCCA AGGTAAAGAC TTCAACGCCA 
CAAACGGGAA CGCCCCAGTC GGCGGCGGGT GTAACACCAC CGGTGCCACT GGAAAGGATT 
GAGGAGCGAG AAACCTCGGA CAGTAATACC AACAAGATGC CCTCCACCTC GGGCACCACC 
AGCAGTACGG GCGGTGTGGG TGGAGGCGGG GAACAAGACG GTGTTGGTGC CACCAAAAAG 
AACGCGGGGG ATGACGATGC CAACAGGGAG GAGAAGAGTT TGGCGGCCAG CCGAAACTCC 
AGCAAAAGGG CATTCATTCA TTCGATAGGA AAGCACTTCA AGAGCAAGAA GGCTCTACCC 
CTGATCCTTG GAGTGGGTGG ACGGCAGAAG TCCAAGTCGG AGAATCGTGC TCGCAAAGCG 
TTTCGCACGA TCTCGTTCAT TTTGGGCTGC TTCGTGGCCT GCTGGACACC CTACCATGTG 
TTGGCCCTCG TCGAGGGATT CTGCCGGCAT CCGCCCTGCA TCAATGAACA CCTCTACATG 
TTCTCGTACT TCCTCTGCTA CGCCAACAGC CCGATGAATC CTTTTTGCTA CGCTCTGGCC 
AATCAGCAGT TCAAGAAGAC CTTTACGCGG ATCCTCAAGG GGGATCTGCA CATGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649764.1
CDS	447..3506
Translation	MRSLNKSAGEEELTGLNGNSSDSIYTIKNKWITDWSLRRERDKLCSFIFKRVLTHTECKRLLEAAKIYGESSDFNSWPVNEQLQWYRNFDVNNTNLLLAVRNQSSDGGSTMSGSSSDSEILGPVLPPFALWQTILIAICLAICIILTVGGNILVLLAFIVDRNIRQPSNYFIASLAATDMLIGTVSMPFYTIYVLKGYWDLGPMLCDLWLSVDYTVCLVSQYTVLLITIDRYCSVKIAAKYRSWRTRTRVIYMVTITWIIPALLFFISIFGWEHFTGKRDLLPGQCAVQFLKDPIFNTALIIGYYWTTLIVLFVLYAGIYKTAYDMQKRSEAKQRKMQSMVALSAGAMSGMAGHAAGIGVIEEKILKTKVELAGDQTDLDSACTTVIKRLSGSGQANPLAVATEEVEKMTPEQRRASAAKIQEEAKKREAAVDAEKSERSSSPAFDSDEESSVNQAQQLITQQKLNNMRKRSSIGLVFGAQAALLATRGKGNLQKSTTNSKSIEAMHQYHHHQQHHHNQSPLQRAQSKEEMRSLHHHQNQQQPHHQHHQNQPNPPLDRPKRTSCSTLSQIAEHDRLVDLSAPPTLLNTSDPLSPVDLAPIEVPSDQVHQGLVQTILPPPDAFQCPTPLSDDYSDRPFGNSSGNSELALTYDLMTNSELRYMDESSAMLASVTANSTTSPNDSVQGKPPVLPPPPPARRNPPKSNLNQSLSQTPSQNQSPSQSLSLNLNPNHNQSQSQSIEEAVAIEKRLLVSYTGIEDFAKVRRESCIEAICLLDVPGKLAADCPHRRASSPMETSSKDAILYSPMPPMPLSPKVKTSTPQTGTPQSAAGVTPPVPLERIEERETSDSNTNKMPSTSGTTSSTGGVGGGGEQDGVGATKKNAGDDDANREEKSLAASRNSSKRAFIHSIGKHFKSKKALPLILGVGGRQKSKSENRARKAFRTISFILGCFVACWTPYHVLALVEGFCRHPPCINEHLYMFSYFLCYANSPMNPFCYALANQQFKKTFTRILKGDLHMT

Target ORF information:

RefSeq Version	NM_141507.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster muscarinic acetylcholine Receptor, B-type, transcript variant A (mAChR-B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141507.2

ORF Insert Sequence:

ATGCGGTCGC TTAACAAGTC GGCGGGCGAG GAGGAGCTGA CTGGCCTGAA TGGCAACTCC 
AGCGACTCCA TCTATACCAT CAAGAACAAG TGGATCACCG ACTGGAGCCT TAGAAGGGAG 
CGGGATAAGC TGTGCAGCTT CATTTTCAAG CGAGTCCTGA CGCACACGGA GTGCAAACGT 
CTGTTGGAGG CGGCCAAGAT CTATGGCGAG AGCAGTGACT TCAATTCCTG GCCGGTTAAT 
GAACAGCTGC AATGGTACAG GAACTTCGAT GTGAACAATA CGAACCTTCT GTTGGCTGTT 
AGAAATCAAT CCTCTGATGG CGGTTCCACC ATGTCGGGAT CCAGTTCCGA CTCGGAGATT 
CTTGGTCCTG TTTTGCCACC ATTTGCACTG TGGCAGACGA TCCTGATAGC CATATGCCTG 
GCGATCTGTA TCATTCTTAC GGTGGGCGGT AATATCCTGG TGCTACTGGC CTTCATCGTG 
GACAGGAATA TTCGGCAGCC CAGCAACTAC TTTATTGCCT CCCTAGCAGC CACGGATATG 
CTAATAGGAA CCGTTTCGAT GCCCTTCTAC ACGATATACG TGCTCAAGGG TTATTGGGAC 
CTGGGTCCGA TGCTCTGCGA TCTCTGGCTT TCCGTCGACT ATACTGTGTG TCTGGTGTCC 
CAGTACACGG TGCTGTTGAT CACGATAGAT CGCTACTGCT CTGTGAAGAT TGCCGCCAAG 
TACCGGAGTT GGAGGACGAG AACCAGAGTG ATATATATGG TCACAATCAC TTGGATCATT 
CCCGCTCTGC TCTTCTTCAT CTCGATTTTT GGATGGGAGC ACTTCACCGG CAAGCGGGAT 
CTACTTCCTG GCCAGTGCGC CGTTCAGTTT CTCAAGGATC CGATCTTTAA TACGGCCCTA 
ATCATTGGCT ACTATTGGAC CACACTAATT GTGCTTTTTG TCTTATATGC CGGGATCTAC 
AAAACAGCCT ATGATATGCA AAAGCGCAGT GAGGCGAAGC AACGGAAAAT GCAATCCATG 
GTGGCACTGA GTGCTGGAGC GATGTCCGGA ATGGCAGGTC ATGCAGCTGG CATTGGTGTG 
ATTGAGGAGA AAATCCTAAA GACCAAAGTG GAACTTGCGG GAGATCAAAC CGATTTGGAT 
AGTGCCTGCA CCACAGTGAT TAAGAGATTA AGTGGTTCCG GTCAGGCAAA TCCTCTGGCC 
GTGGCCACCG AGGAAGTGGA GAAGATGACA CCGGAACAGA GGAGAGCGTC GGCAGCAAAA 
ATCCAGGAGG AGGCGAAAAA ACGTGAGGCA GCCGTGGATG CGGAAAAGAG CGAGAGATCC 
AGCAGTCCTG CCTTCGATTC CGATGAGGAA TCATCCGTCA ACCAGGCCCA GCAGTTGATC 
ACCCAGCAGA AATTGAACAA CATGAGGAAG CGGTCATCGA TTGGTCTGGT CTTTGGGGCA 
CAGGCCGCCC TGCTGGCCAC TCGTGGCAAA GGTAACTTGC AGAAGAGCAC CACTAACTCC 
AAGTCGATCG AGGCCATGCA CCAGTACCAC CACCACCAAC AGCACCATCA CAACCAGAGT 
CCGTTACAGC GGGCGCAGAG CAAGGAAGAG ATGCGCTCCC TGCACCACCA CCAGAACCAG 
CAACAACCGC ACCACCAGCA CCACCAAAAC CAGCCGAACC CACCGCTGGA CAGGCCCAAG 
CGCACATCCT GCTCCACACT GTCCCAGATA GCCGAGCATG ATCGTTTGGT CGACCTGTCC 
GCTCCTCCAA CATTACTCAA CACCAGTGAT CCCCTGTCCC CCGTGGACTT GGCACCCATC 
GAGGTCCCTA GCGATCAAGT GCATCAGGGT CTGGTGCAGA CCATTTTGCC ACCTCCCGAC 
GCCTTTCAGT GCCCCACCCC GCTCTCCGAT GACTACTCGG ATCGTCCCTT TGGGAATAGT 
TCCGGTAACA GTGAACTGGC CCTGACCTAT GACCTGATGA CGAACTCGGA GCTACGCTAT 
ATGGACGAGA GCTCCGCCAT GCTGGCCAGT GTGACGGCCA ACTCGACGAC CTCCCCGAAT 
GACAGCGTCC AGGGGAAACC ACCAGTCCTG CCTCCACCAC CGCCCGCACG CCGAAATCCA 
CCAAAATCGA ACCTGAACCA GAGCCTCAGT CAAACTCCCA GCCAAAACCA GAGTCCCAGC 
CAAAGCCTGA GCCTCAACCT GAATCCGAAT CACAACCAGA GTCAGAGTCA GAGTATCGAG 
GAGGCGGTGG CCATTGAAAA ACGTCTTCTC GTCTCGTACA CAGGAATCGA GGACTTTGCT 
AAGGTGAGGC GGGAGAGCTG CATTGAGGCC ATCTGCCTGT TGGATGTGCC CGGAAAGCTA 
GCTGCGGACT GTCCCCATCG CCGGGCCTCC AGTCCTATGG AGACCAGCAG CAAGGATGCC 
ATACTCTATT CCCCGATGCC ACCAATGCCA CTCTCACCCA AGGTAAAGAC TTCAACGCCA 
CAAACGGGAA CGCCCCAGTC GGCGGCGGGT GTAACACCAC CGGTGCCACT GGAAAGGATT 
GAGGAGCGAG AAACCTCGGA CAGTAATACC AACAAGATGC CCTCCACCTC GGGCACCACC 
AGCAGTACGG GCGGTGTGGG TGGAGGCGGG GAACAAGACG GTGTTGGTGC CACCAAAAAG 
AACGCGGGGG ATGACGATGC CAACAGGGAG GAGAAGAGTT TGGCGGCCAG CCGAAACTCC 
AGCAAAAGGG CATTCATTCA TTCGATAGGA AAGCACTTCA AGAGCAAGAA GGCTCTACCC 
CTGATCCTTG GAGTGGGTGG ACGGCAGAAG TCCAAGTCGG AGAATCGTGC TCGCAAAGCG 
TTTCGCACGA TCTCGTTCAT TTTGGGCTGC TTCGTGGCCT GCTGGACACC CTACCATGTG 
TTGGCCCTCG TCGAGGGATT CTGCCGGCAT CCGCCCTGCA TCAATGAACA CCTCTACATG 
TTCTCGTACT TCCTCTGCTA CGCCAACAGC CCGATGAATC CTTTTTGCTA CGCTCTGGCC 
AATCAGCAGT TCAAGAAGAC CTTTACGCGG ATCCTCAAGG GGGATCTGCA CATGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mAChR-B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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