dsx cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dsx
Entrez Gene ID	40940
Full Name	doublesex
Synonyms	CG11094,DSX,Dmdsx,Dmel\CG11094,Dsx,Hr,dsxF,dsxM,ix-62c
General protein information	Preferred Names doublesex Names CG11094 gene product from transcript CG11094-RA CG11094-PA CG11094-PB CG11094-PC CG11094-PD CG11094-PE CG11094-PF double sex dsx-PA dsx-PB dsx-PC dsx-PD dsx-PE dsx-PF hermaphrodite intersex-62c
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84E5-84E6\|3-48.1 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_169203.2	NP_731198.1	doublesex, isoform B
NM_001275423.1	NP_001262352.1	doublesex, isoform D
NM_001275424.1	NP_001262353.1	doublesex, isoform E
NM_169202.1	NP_731197.1	doublesex, isoform A
NM_079548.5	NP_524272.4	doublesex, isoform C
NM_001300291.1	NP_001287220.1	doublesex, isoform F

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004050	XP_309601.4
Drosophila melanogaster (fruit fly)	dsx	NP_524272.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The sex determination genes fruitless and doublesex, and a sex-specific P1-DN1 neuronal feedback govern the interaction between sleep and sexual behavior.
Title: Genetic and neuronal mechanisms governing the sex-specific interaction between sleep and sexual behaviors in Drosophila.

Identification of the Doublesex protein binding sites that activate expression of lozenge in the female genital disc in Drosophila melanogaster has been reported.
Title: Identification of the Doublesex protein binding sites that activate expression of lozenge in the female genital disc in Drosophila melanogaster.

Sex differences in Drosophila somatic gene expression controlled by variation and regulation by doublesex have been scrutinized.
Title: Sex Differences in Drosophila Somatic Gene Expression: Variation and Regulation by doublesex.

doublesex is required in the TN1A neurons during development to increase the density of the TN1A arbors that interact with dendrites of the hg1 motoneuron.
Title: Doublesex Regulates the Connectivity of a Neural Circuit Controlling Drosophila Male Courtship Song.

The Flavin-containing monooxygenase-2 (Fmo-2) gene, a likely direct dsx target, was investigated to elucidate how sexually dimorphic expression and its evolution are brought about.
Title: Constraints on the evolution of a doublesex target gene arising from doublesex's pleiotropic deployment.

The following dsx gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dsx cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04220	NM_001275424.1 Latest version!	Drosophila melanogaster doublesex (dsx), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa04221	NM_169202.1 Latest version!	Drosophila melanogaster doublesex (dsx), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa04221	NM_001275423.1 Latest version!	Drosophila melanogaster doublesex (dsx), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa07972	NM_079548.5 Latest version!	Drosophila melanogaster doublesex (dsx), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07972	NM_001300291.1 Latest version!	Drosophila melanogaster doublesex (dsx), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07972	NM_169203.2 Latest version!	Drosophila melanogaster doublesex (dsx), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dsx ( NM_001275424.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262353.1 Drosophila melanogaster doublesex (dsx), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa04220
Clone ID Related Accession (Same CDS sequence)	NM_001275424.1
Accession Version	NM_001275424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	doublesex, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTCGG AGGAGAACTG GAATAGCGAC ACGATGTCCG ACTCGGACAT GATCGACTCA 
AAGAACGACG TCTGTGGCGG AGCCTCCAGT TCCAGCGGCA GCTCGATTTC GCCGAGGACA 
CCACCGAACT GCGCCCGCTG CCGCAATCAT GGCCTAAAGA TCACCCTAAA GGGACACAAG 
CGGTACTGCA AGTTCCGCTA CTGCACGTGC GAGAAGTGCC GACTGACGGC GGACCGCCAG 
CGGGTGATGG CTCTGCAAAC GGCCTTGAGG CGAGCCCAGG CGCAGGATGA GCAGCGGGCG 
CTGCACATGC ACGAGGTGCC GCCTGCCAAT CCGGCGGCCA CGACCCTCCT GAGTCATCAC 
CATCATGTCG CAGCTCCCGC TCATGTCCAC GCCCACCATG TGCACGCCCA TCACGCACAC 
GGAGGACACC ACTCGCATCA CGGACATGTC CTGCACCACC AGCAGGCAGC GGCGGCCGCA 
GCAGCAGCTC CGTCGGCTCC AGCCTCCCAT CTGGGTGGCT CCAGCACGGC CGCTTCCAGC 
ATCCACGGTC ACGCCCACGC GCACCACGTC CACATGGCAG CTGCTGCAGC CGCCTCGGTG 
GCTCAGCACC AGCACCAAAG CCACCCACAC TCGCACCACC ACCACCACCA GAACCACCAC 
CAGCATCCGC ATCAGCAACC GGCCACGCAG ACCGCCCTGC GATCTCCGCC GCACAGCGAC 
CATGGTGGCA GTGTGGGTCC GGCCACCAGC AGCTCCGGCG GTGGAGCACC CAGTTCCAGC 
AATGCGGCAG CGGCCACCTC GAGCAACGGA TCCAGCGGCG GTGGAGGAGG AGGCGGAGGG 
GGCAGCTCGG GTGGCGGGGC AGGAGGTGGT AGGTCATCGG GAACATCGGT GATCACTAGC 
GCCGATCACC ACATGACCAC GGTGCCTACG CCCGCCCAAT CGCTGGAGGG GTCCTGCGAC 
TCGTCGTCGC CCTCGCCGTC GTCCACTTCC GGTGCAGCCA TTTTGCCGAT CTCAGTTTCC 
GTCAATCGAA AGAACGGCGC CAATGTGCCC TTGGGCCAAG ACGTTTTCCT AGACTATTGC 
CAAAAGCTAT TAGAAAAATT CCGCTATCCT TGGGAGCTGA TGCCACTCAT GTATGTGATA 
TTAAAGGACG CAGACGCCAA CATTGAAGAG GCTTCCCGGC GAATCGAAGA GGCTCGAGTG 
GAAATAAATC GCACTGTAGC CCAGATCTAC TACAACTACT ACACCCCGAT GGCCCTGGTG 
AACGGGGCGC CCATGTACCT GACCTACCCG AGCATCGAAC AGGGTCGCTA TGGGGCGCAC 
TTCACCCATC TGCCGCTCAC ACAGATTTGT CCACCGACTC CAGAGCCGCT GGCCCTCAGC 
CGCTCCCCGA GCAGTCCCAG TGGACCGTCG GCTGTCCACA ACCAAAAGCC CTCCCGACCG 
GGCAGCAGCA ATGGCACCGT CCACTCCGCG GCCTCACCCA CAATGGTCAC CACGATGGCC 
ACGACCTCCT CCACGCCCAC GCTCAGCCGC CGTCAGAGAT CGCGCTCGGC CACGCCCACC 
ACTCCGCCAC CACCGCCACC GGCGCACAGC AGCAGCAACG GAGCCTACCA CCACGGCCAC 
CACCTGGTCA GCTCCACGGC TGCCACGTAG CAGTATCGCA ACGTTGCTGC CGCCGTGGCA 
GCAGCAGCAG CGGCCGCTGT CCTCTTCGTC GATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262353.1
CDS	1264..2982
Translation	MVSEENWNSDTMSDSDMIDSKNDVCGGASSSSGSSISPRTPPNCARCRNHGLKITLKGHKRYCKFRYCTCEKCRLTADRQRVMALQTALRRAQAQDEQRALHMHEVPPANPAATTLLSHHHHVAAPAHVHAHHVHAHHAHGGHHSHHGHVLHHQQAAAAAAAAPSAPASHLGGSSTAASSIHGHAHAHHVHMAAAAAASVAQHQHQSHPHSHHHHHQNHHQHPHQQPATQTALRSPPHSDHGGSVGPATSSSGGGAPSSSNAAAATSSNGSSGGGGGGGGGSSGGGAGGGRSSGTSVITSADHHMTTVPTPAQSLEGSCDSSSPSPSSTSGAAILPISVSVNRKNGANVPLGQDVFLDYCQKLLEKFRYPWELMPLMYVILKDADANIEEASRRIEEARVEINRTVAQIYYNYYTPMALVNGAPMYLTYPSIEQGRYGAHFTHLPLTQICPPTPEPLALSRSPSSPSGPSAVHNQKPSRPGSSNGTVHSAASPTMVTTMATTSSTPTLSRRQRSRSATPTTPPPPPPAHSSSNGAYHHGHHLVSSTAAT*QYRNVAAAVAAAAAAAVLFVDN

Target ORF information:

RefSeq Version	NM_001275424.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster doublesex (dsx), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275424.1

ORF Insert Sequence:

ATGGTTTCGG AGGAGAACTG GAATAGCGAC ACGATGTCCG ACTCGGACAT GATCGACTCA 
AAGAACGACG TCTGTGGCGG AGCCTCCAGT TCCAGCGGCA GCTCGATTTC GCCGAGGACA 
CCACCGAACT GCGCCCGCTG CCGCAATCAT GGCCTAAAGA TCACCCTAAA GGGACACAAG 
CGGTACTGCA AGTTCCGCTA CTGCACGTGC GAGAAGTGCC GACTGACGGC GGACCGCCAG 
CGGGTGATGG CTCTGCAAAC GGCCTTGAGG CGAGCCCAGG CGCAGGATGA GCAGCGGGCG 
CTGCACATGC ACGAGGTGCC GCCTGCCAAT CCGGCGGCCA CGACCCTCCT GAGTCATCAC 
CATCATGTCG CAGCTCCCGC TCATGTCCAC GCCCACCATG TGCACGCCCA TCACGCACAC 
GGAGGACACC ACTCGCATCA CGGACATGTC CTGCACCACC AGCAGGCAGC GGCGGCCGCA 
GCAGCAGCTC CGTCGGCTCC AGCCTCCCAT CTGGGTGGCT CCAGCACGGC CGCTTCCAGC 
ATCCACGGTC ACGCCCACGC GCACCACGTC CACATGGCAG CTGCTGCAGC CGCCTCGGTG 
GCTCAGCACC AGCACCAAAG CCACCCACAC TCGCACCACC ACCACCACCA GAACCACCAC 
CAGCATCCGC ATCAGCAACC GGCCACGCAG ACCGCCCTGC GATCTCCGCC GCACAGCGAC 
CATGGTGGCA GTGTGGGTCC GGCCACCAGC AGCTCCGGCG GTGGAGCACC CAGTTCCAGC 
AATGCGGCAG CGGCCACCTC GAGCAACGGA TCCAGCGGCG GTGGAGGAGG AGGCGGAGGG 
GGCAGCTCGG GTGGCGGGGC AGGAGGTGGT AGGTCATCGG GAACATCGGT GATCACTAGC 
GCCGATCACC ACATGACCAC GGTGCCTACG CCCGCCCAAT CGCTGGAGGG GTCCTGCGAC 
TCGTCGTCGC CCTCGCCGTC GTCCACTTCC GGTGCAGCCA TTTTGCCGAT CTCAGTTTCC 
GTCAATCGAA AGAACGGCGC CAATGTGCCC TTGGGCCAAG ACGTTTTCCT AGACTATTGC 
CAAAAGCTAT TAGAAAAATT CCGCTATCCT TGGGAGCTGA TGCCACTCAT GTATGTGATA 
TTAAAGGACG CAGACGCCAA CATTGAAGAG GCTTCCCGGC GAATCGAAGA GGCTCGAGTG 
GAAATAAATC GCACTGTAGC CCAGATCTAC TACAACTACT ACACCCCGAT GGCCCTGGTG 
AACGGGGCGC CCATGTACCT GACCTACCCG AGCATCGAAC AGGGTCGCTA TGGGGCGCAC 
TTCACCCATC TGCCGCTCAC ACAGATTTGT CCACCGACTC CAGAGCCGCT GGCCCTCAGC 
CGCTCCCCGA GCAGTCCCAG TGGACCGTCG GCTGTCCACA ACCAAAAGCC CTCCCGACCG 
GGCAGCAGCA ATGGCACCGT CCACTCCGCG GCCTCACCCA CAATGGTCAC CACGATGGCC 
ACGACCTCCT CCACGCCCAC GCTCAGCCGC CGTCAGAGAT CGCGCTCGGC CACGCCCACC 
ACTCCGCCAC CACCGCCACC GGCGCACAGC AGCAGCAACG GAGCCTACCA CCACGGCCAC 
CACCTGGTCA GCTCCACGGC TGCCACGTAG CAGTATCGCA ACGTTGCTGC CGCCGTGGCA 
GCAGCAGCAG CGGCCGCTGT CCTCTTCGTC GATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dsx ( NM_001275423.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262352.1 Drosophila melanogaster doublesex (dsx), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa04221
Clone ID Related Accession (Same CDS sequence)	NM_001275423.1 , NM_169202.1
Accession Version	NM_001275423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	doublesex, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTCGG AGGAGAACTG GAATAGCGAC ACGATGTCCG ACTCGGACAT GATCGACTCA 
AAGAACGACG TCTGTGGCGG AGCCTCCAGT TCCAGCGGCA GCTCGATTTC GCCGAGGACA 
CCACCGAACT GCGCCCGCTG CCGCAATCAT GGCCTAAAGA TCACCCTAAA GGGACACAAG 
CGGTACTGCA AGTTCCGCTA CTGCACGTGC GAGAAGTGCC GACTGACGGC GGACCGCCAG 
CGGGTGATGG CTCTGCAAAC GGCCTTGAGG CGAGCCCAGG CGCAGGATGA GCAGCGGGCG 
CTGCACATGC ACGAGGTGCC GCCTGCCAAT CCGGCGGCCA CGACCCTCCT GAGTCATCAC 
CATCATGTCG CAGCTCCCGC TCATGTCCAC GCCCACCATG TGCACGCCCA TCACGCACAC 
GGAGGACACC ACTCGCATCA CGGACATGTC CTGCACCACC AGCAGGCAGC GGCGGCCGCA 
GCAGCAGCTC CGTCGGCTCC AGCCTCCCAT CTGGGTGGCT CCAGCACGGC CGCTTCCAGC 
ATCCACGGTC ACGCCCACGC GCACCACGTC CACATGGCAG CTGCTGCAGC CGCCTCGGTG 
GCTCAGCACC AGCACCAAAG CCACCCACAC TCGCACCACC ACCACCACCA GAACCACCAC 
CAGCATCCGC ATCAGCAACC GGCCACGCAG ACCGCCCTGC GATCTCCGCC GCACAGCGAC 
CATGGTGGCA GTGTGGGTCC GGCCACCAGC AGCTCCGGCG GTGGAGCACC CAGTTCCAGC 
AATGCGGCAG CGGCCACCTC GAGCAACGGA TCCAGCGGCG GTGGAGGAGG AGGCGGAGGG 
GGCAGCTCGG GTGGCGGGGC AGGAGGTGGT AGGTCATCGG GAACATCGGT GATCACTAGC 
GCCGATCACC ACATGACCAC GGTGCCTACG CCCGCCCAAT CGCTGGAGGG GTCCTGCGAC 
TCGTCGTCGC CCTCGCCGTC GTCCACTTCC GGTGCAGCCA TTTTGCCGAT CTCAGTTTCC 
GTCAATCGAA AGAACGGCGC CAATGTGCCC TTGGGCCAAG ACGTTTTCCT AGACTATTGC 
CAAAAGCTAT TAGAAAAATT CCGCTATCCT TGGGAGCTGA TGCCACTCAT GTATGTGATA 
TTAAAGGACG CAGACGCCAA CATTGAAGAG GCTTCCCGGC GAATCGAAGA GGCTCGAGTG 
GAAATAAATC GCACTGTAGC CCAGATCTAC TACAACTACT ACACCCCGAT GGCCCTGGTG 
AACGGGGCGC CCATGTACCT GACCTACCCG AGCATCGAAC AGGGTCGCTA TGGGGCGCAC 
TTCACCCATC TGCCGCTCAC ACAGATTTGT CCACCGACTC CAGAGCCGCT GGCCCTCAGC 
CGCTCCCCGA GCAGTCCCAG TGGACCGTCG GCTGTCCACA ACCAAAAGCC CTCCCGACCG 
GGCAGCAGCA ATGGCACCGT CCACTCCGCG GCCTCACCCA CAATGGTCAC CACGATGGCC 
ACGACCTCCT CCACGCCCAC GCTCAGCCGC CGTCAGAGAT CGCGCTCGGC CACGCCCACC 
ACTCCGCCAC CACCGCCACC GGCGCACAGC AGCAGCAACG GAGCCTACCA CCACGGCCAC 
CACCTGGTCA GCTCCACGGC TGCCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262352.1
CDS	875..2524
Translation	MVSEENWNSDTMSDSDMIDSKNDVCGGASSSSGSSISPRTPPNCARCRNHGLKITLKGHKRYCKFRYCTCEKCRLTADRQRVMALQTALRRAQAQDEQRALHMHEVPPANPAATTLLSHHHHVAAPAHVHAHHVHAHHAHGGHHSHHGHVLHHQQAAAAAAAAPSAPASHLGGSSTAASSIHGHAHAHHVHMAAAAAASVAQHQHQSHPHSHHHHHQNHHQHPHQQPATQTALRSPPHSDHGGSVGPATSSSGGGAPSSSNAAAATSSNGSSGGGGGGGGGSSGGGAGGGRSSGTSVITSADHHMTTVPTPAQSLEGSCDSSSPSPSSTSGAAILPISVSVNRKNGANVPLGQDVFLDYCQKLLEKFRYPWELMPLMYVILKDADANIEEASRRIEEARVEINRTVAQIYYNYYTPMALVNGAPMYLTYPSIEQGRYGAHFTHLPLTQICPPTPEPLALSRSPSSPSGPSAVHNQKPSRPGSSNGTVHSAASPTMVTTMATTSSTPTLSRRQRSRSATPTTPPPPPPAHSSSNGAYHHGHHLVSSTAAT

Target ORF information:

RefSeq Version	NM_001275423.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster doublesex (dsx), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275423.1

ORF Insert Sequence:

ATGGTTTCGG AGGAGAACTG GAATAGCGAC ACGATGTCCG ACTCGGACAT GATCGACTCA 
AAGAACGACG TCTGTGGCGG AGCCTCCAGT TCCAGCGGCA GCTCGATTTC GCCGAGGACA 
CCACCGAACT GCGCCCGCTG CCGCAATCAT GGCCTAAAGA TCACCCTAAA GGGACACAAG 
CGGTACTGCA AGTTCCGCTA CTGCACGTGC GAGAAGTGCC GACTGACGGC GGACCGCCAG 
CGGGTGATGG CTCTGCAAAC GGCCTTGAGG CGAGCCCAGG CGCAGGATGA GCAGCGGGCG 
CTGCACATGC ACGAGGTGCC GCCTGCCAAT CCGGCGGCCA CGACCCTCCT GAGTCATCAC 
CATCATGTCG CAGCTCCCGC TCATGTCCAC GCCCACCATG TGCACGCCCA TCACGCACAC 
GGAGGACACC ACTCGCATCA CGGACATGTC CTGCACCACC AGCAGGCAGC GGCGGCCGCA 
GCAGCAGCTC CGTCGGCTCC AGCCTCCCAT CTGGGTGGCT CCAGCACGGC CGCTTCCAGC 
ATCCACGGTC ACGCCCACGC GCACCACGTC CACATGGCAG CTGCTGCAGC CGCCTCGGTG 
GCTCAGCACC AGCACCAAAG CCACCCACAC TCGCACCACC ACCACCACCA GAACCACCAC 
CAGCATCCGC ATCAGCAACC GGCCACGCAG ACCGCCCTGC GATCTCCGCC GCACAGCGAC 
CATGGTGGCA GTGTGGGTCC GGCCACCAGC AGCTCCGGCG GTGGAGCACC CAGTTCCAGC 
AATGCGGCAG CGGCCACCTC GAGCAACGGA TCCAGCGGCG GTGGAGGAGG AGGCGGAGGG 
GGCAGCTCGG GTGGCGGGGC AGGAGGTGGT AGGTCATCGG GAACATCGGT GATCACTAGC 
GCCGATCACC ACATGACCAC GGTGCCTACG CCCGCCCAAT CGCTGGAGGG GTCCTGCGAC 
TCGTCGTCGC CCTCGCCGTC GTCCACTTCC GGTGCAGCCA TTTTGCCGAT CTCAGTTTCC 
GTCAATCGAA AGAACGGCGC CAATGTGCCC TTGGGCCAAG ACGTTTTCCT AGACTATTGC 
CAAAAGCTAT TAGAAAAATT CCGCTATCCT TGGGAGCTGA TGCCACTCAT GTATGTGATA 
TTAAAGGACG CAGACGCCAA CATTGAAGAG GCTTCCCGGC GAATCGAAGA GGCTCGAGTG 
GAAATAAATC GCACTGTAGC CCAGATCTAC TACAACTACT ACACCCCGAT GGCCCTGGTG 
AACGGGGCGC CCATGTACCT GACCTACCCG AGCATCGAAC AGGGTCGCTA TGGGGCGCAC 
TTCACCCATC TGCCGCTCAC ACAGATTTGT CCACCGACTC CAGAGCCGCT GGCCCTCAGC 
CGCTCCCCGA GCAGTCCCAG TGGACCGTCG GCTGTCCACA ACCAAAAGCC CTCCCGACCG 
GGCAGCAGCA ATGGCACCGT CCACTCCGCG GCCTCACCCA CAATGGTCAC CACGATGGCC 
ACGACCTCCT CCACGCCCAC GCTCAGCCGC CGTCAGAGAT CGCGCTCGGC CACGCCCACC 
ACTCCGCCAC CACCGCCACC GGCGCACAGC AGCAGCAACG GAGCCTACCA CCACGGCCAC 
CACCTGGTCA GCTCCACGGC TGCCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dsx ( NM_169202.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731197.1 Drosophila melanogaster doublesex (dsx), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04221
Clone ID Related Accession (Same CDS sequence)	NM_001275423.1 , NM_169202.1
Accession Version	NM_169202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	doublesex, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTCGG AGGAGAACTG GAATAGCGAC ACGATGTCCG ACTCGGACAT GATCGACTCA 
AAGAACGACG TCTGTGGCGG AGCCTCCAGT TCCAGCGGCA GCTCGATTTC GCCGAGGACA 
CCACCGAACT GCGCCCGCTG CCGCAATCAT GGCCTAAAGA TCACCCTAAA GGGACACAAG 
CGGTACTGCA AGTTCCGCTA CTGCACGTGC GAGAAGTGCC GACTGACGGC GGACCGCCAG 
CGGGTGATGG CTCTGCAAAC GGCCTTGAGG CGAGCCCAGG CGCAGGATGA GCAGCGGGCG 
CTGCACATGC ACGAGGTGCC GCCTGCCAAT CCGGCGGCCA CGACCCTCCT GAGTCATCAC 
CATCATGTCG CAGCTCCCGC TCATGTCCAC GCCCACCATG TGCACGCCCA TCACGCACAC 
GGAGGACACC ACTCGCATCA CGGACATGTC CTGCACCACC AGCAGGCAGC GGCGGCCGCA 
GCAGCAGCTC CGTCGGCTCC AGCCTCCCAT CTGGGTGGCT CCAGCACGGC CGCTTCCAGC 
ATCCACGGTC ACGCCCACGC GCACCACGTC CACATGGCAG CTGCTGCAGC CGCCTCGGTG 
GCTCAGCACC AGCACCAAAG CCACCCACAC TCGCACCACC ACCACCACCA GAACCACCAC 
CAGCATCCGC ATCAGCAACC GGCCACGCAG ACCGCCCTGC GATCTCCGCC GCACAGCGAC 
CATGGTGGCA GTGTGGGTCC GGCCACCAGC AGCTCCGGCG GTGGAGCACC CAGTTCCAGC 
AATGCGGCAG CGGCCACCTC GAGCAACGGA TCCAGCGGCG GTGGAGGAGG AGGCGGAGGG 
GGCAGCTCGG GTGGCGGGGC AGGAGGTGGT AGGTCATCGG GAACATCGGT GATCACTAGC 
GCCGATCACC ACATGACCAC GGTGCCTACG CCCGCCCAAT CGCTGGAGGG GTCCTGCGAC 
TCGTCGTCGC CCTCGCCGTC GTCCACTTCC GGTGCAGCCA TTTTGCCGAT CTCAGTTTCC 
GTCAATCGAA AGAACGGCGC CAATGTGCCC TTGGGCCAAG ACGTTTTCCT AGACTATTGC 
CAAAAGCTAT TAGAAAAATT CCGCTATCCT TGGGAGCTGA TGCCACTCAT GTATGTGATA 
TTAAAGGACG CAGACGCCAA CATTGAAGAG GCTTCCCGGC GAATCGAAGA GGCTCGAGTG 
GAAATAAATC GCACTGTAGC CCAGATCTAC TACAACTACT ACACCCCGAT GGCCCTGGTG 
AACGGGGCGC CCATGTACCT GACCTACCCG AGCATCGAAC AGGGTCGCTA TGGGGCGCAC 
TTCACCCATC TGCCGCTCAC ACAGATTTGT CCACCGACTC CAGAGCCGCT GGCCCTCAGC 
CGCTCCCCGA GCAGTCCCAG TGGACCGTCG GCTGTCCACA ACCAAAAGCC CTCCCGACCG 
GGCAGCAGCA ATGGCACCGT CCACTCCGCG GCCTCACCCA CAATGGTCAC CACGATGGCC 
ACGACCTCCT CCACGCCCAC GCTCAGCCGC CGTCAGAGAT CGCGCTCGGC CACGCCCACC 
ACTCCGCCAC CACCGCCACC GGCGCACAGC AGCAGCAACG GAGCCTACCA CCACGGCCAC 
CACCTGGTCA GCTCCACGGC TGCCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731197.1
CDS	1264..2913
Translation	MVSEENWNSDTMSDSDMIDSKNDVCGGASSSSGSSISPRTPPNCARCRNHGLKITLKGHKRYCKFRYCTCEKCRLTADRQRVMALQTALRRAQAQDEQRALHMHEVPPANPAATTLLSHHHHVAAPAHVHAHHVHAHHAHGGHHSHHGHVLHHQQAAAAAAAAPSAPASHLGGSSTAASSIHGHAHAHHVHMAAAAAASVAQHQHQSHPHSHHHHHQNHHQHPHQQPATQTALRSPPHSDHGGSVGPATSSSGGGAPSSSNAAAATSSNGSSGGGGGGGGGSSGGGAGGGRSSGTSVITSADHHMTTVPTPAQSLEGSCDSSSPSPSSTSGAAILPISVSVNRKNGANVPLGQDVFLDYCQKLLEKFRYPWELMPLMYVILKDADANIEEASRRIEEARVEINRTVAQIYYNYYTPMALVNGAPMYLTYPSIEQGRYGAHFTHLPLTQICPPTPEPLALSRSPSSPSGPSAVHNQKPSRPGSSNGTVHSAASPTMVTTMATTSSTPTLSRRQRSRSATPTTPPPPPPAHSSSNGAYHHGHHLVSSTAAT

Target ORF information:

RefSeq Version	NM_169202.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster doublesex (dsx), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169202.1

ORF Insert Sequence:

ATGGTTTCGG AGGAGAACTG GAATAGCGAC ACGATGTCCG ACTCGGACAT GATCGACTCA 
AAGAACGACG TCTGTGGCGG AGCCTCCAGT TCCAGCGGCA GCTCGATTTC GCCGAGGACA 
CCACCGAACT GCGCCCGCTG CCGCAATCAT GGCCTAAAGA TCACCCTAAA GGGACACAAG 
CGGTACTGCA AGTTCCGCTA CTGCACGTGC GAGAAGTGCC GACTGACGGC GGACCGCCAG 
CGGGTGATGG CTCTGCAAAC GGCCTTGAGG CGAGCCCAGG CGCAGGATGA GCAGCGGGCG 
CTGCACATGC ACGAGGTGCC GCCTGCCAAT CCGGCGGCCA CGACCCTCCT GAGTCATCAC 
CATCATGTCG CAGCTCCCGC TCATGTCCAC GCCCACCATG TGCACGCCCA TCACGCACAC 
GGAGGACACC ACTCGCATCA CGGACATGTC CTGCACCACC AGCAGGCAGC GGCGGCCGCA 
GCAGCAGCTC CGTCGGCTCC AGCCTCCCAT CTGGGTGGCT CCAGCACGGC CGCTTCCAGC 
ATCCACGGTC ACGCCCACGC GCACCACGTC CACATGGCAG CTGCTGCAGC CGCCTCGGTG 
GCTCAGCACC AGCACCAAAG CCACCCACAC TCGCACCACC ACCACCACCA GAACCACCAC 
CAGCATCCGC ATCAGCAACC GGCCACGCAG ACCGCCCTGC GATCTCCGCC GCACAGCGAC 
CATGGTGGCA GTGTGGGTCC GGCCACCAGC AGCTCCGGCG GTGGAGCACC CAGTTCCAGC 
AATGCGGCAG CGGCCACCTC GAGCAACGGA TCCAGCGGCG GTGGAGGAGG AGGCGGAGGG 
GGCAGCTCGG GTGGCGGGGC AGGAGGTGGT AGGTCATCGG GAACATCGGT GATCACTAGC 
GCCGATCACC ACATGACCAC GGTGCCTACG CCCGCCCAAT CGCTGGAGGG GTCCTGCGAC 
TCGTCGTCGC CCTCGCCGTC GTCCACTTCC GGTGCAGCCA TTTTGCCGAT CTCAGTTTCC 
GTCAATCGAA AGAACGGCGC CAATGTGCCC TTGGGCCAAG ACGTTTTCCT AGACTATTGC 
CAAAAGCTAT TAGAAAAATT CCGCTATCCT TGGGAGCTGA TGCCACTCAT GTATGTGATA 
TTAAAGGACG CAGACGCCAA CATTGAAGAG GCTTCCCGGC GAATCGAAGA GGCTCGAGTG 
GAAATAAATC GCACTGTAGC CCAGATCTAC TACAACTACT ACACCCCGAT GGCCCTGGTG 
AACGGGGCGC CCATGTACCT GACCTACCCG AGCATCGAAC AGGGTCGCTA TGGGGCGCAC 
TTCACCCATC TGCCGCTCAC ACAGATTTGT CCACCGACTC CAGAGCCGCT GGCCCTCAGC 
CGCTCCCCGA GCAGTCCCAG TGGACCGTCG GCTGTCCACA ACCAAAAGCC CTCCCGACCG 
GGCAGCAGCA ATGGCACCGT CCACTCCGCG GCCTCACCCA CAATGGTCAC CACGATGGCC 
ACGACCTCCT CCACGCCCAC GCTCAGCCGC CGTCAGAGAT CGCGCTCGGC CACGCCCACC 
ACTCCGCCAC CACCGCCACC GGCGCACAGC AGCAGCAACG GAGCCTACCA CCACGGCCAC 
CACCTGGTCA GCTCCACGGC TGCCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dsx ( NM_169203.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731198.1 Drosophila melanogaster doublesex (dsx), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa07972
Clone ID Related Accession (Same CDS sequence)	NM_169203.2 , NM_079548.5 , NM_001300291.1
Accession Version	NM_169203.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	doublesex, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169203.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTCGG AGGAGAACTG GAATAGCGAC ACGATGTCCG ACTCGGACAT GATCGACTCA 
AAGAACGACG TCTGTGGCGG AGCCTCCAGT TCCAGCGGCA GCTCGATTTC GCCGAGGACA 
CCACCGAACT GCGCCCGCTG CCGCAATCAT GGCCTAAAGA TCACCCTAAA GGGACACAAG 
CGGTACTGCA AGTTCCGCTA CTGCACGTGC GAGAAGTGCC GACTGACGGC GGACCGCCAG 
CGGGTGATGG CTCTGCAAAC GGCCTTGAGG CGAGCCCAGG CGCAGGATGA GCAGCGGGCG 
CTGCACATGC ACGAGGTGCC GCCTGCCAAT CCGGCGGCCA CGACCCTCCT GAGTCATCAC 
CATCATGTCG CAGCTCCCGC TCATGTCCAC GCCCACCATG TGCACGCCCA TCACGCACAC 
GGAGGACACC ACTCGCATCA CGGACATGTC CTGCACCACC AGCAGGCAGC GGCGGCCGCA 
GCAGCAGCTC CGTCGGCTCC AGCCTCCCAT CTGGGTGGCT CCAGCACGGC CGCTTCCAGC 
ATCCACGGTC ACGCCCACGC GCACCACGTC CACATGGCAG CTGCTGCAGC CGCCTCGGTG 
GCTCAGCACC AGCACCAAAG CCACCCACAC TCGCACCACC ACCACCACCA GAACCACCAC 
CAGCATCCGC ATCAGCAACC GGCCACGCAG ACCGCCCTGC GATCTCCGCC GCACAGCGAC 
CATGGTGGCA GTGTGGGTCC GGCCACCAGC AGCTCCGGCG GTGGAGCACC CAGTTCCAGC 
AATGCGGCAG CGGCCACCTC GAGCAACGGA TCCAGCGGCG GTGGAGGAGG AGGCGGAGGG 
GGCAGCTCGG GTGGCGGGGC AGGAGGTGGT AGGTCATCGG GAACATCGGT GATCACTAGC 
GCCGATCACC ACATGACCAC GGTGCCTACG CCCGCCCAAT CGCTGGAGGG GTCCTGCGAC 
TCGTCGTCGC CCTCGCCGTC GTCCACTTCC GGTGCAGCCA TTTTGCCGAT CTCAGTTTCC 
GTCAATCGAA AGAACGGCGC CAATGTGCCC TTGGGCCAAG ACGTTTTCCT AGACTATTGC 
CAAAAGCTAT TAGAAAAATT CCGCTATCCT TGGGAGCTGA TGCCACTCAT GTATGTGATA 
TTAAAGGACG CAGACGCCAA CATTGAAGAG GCTTCCCGGC GAATCGAAGA GGGCCAATAC 
GTTGTGAATG AGTACTCCCG TCAACATAAT TTGAATATCT ATGACGGGGG TGAGCTGCGC 
AACACAACGC GGCAATGTGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731198.1
CDS	1264..2547
Translation	MVSEENWNSDTMSDSDMIDSKNDVCGGASSSSGSSISPRTPPNCARCRNHGLKITLKGHKRYCKFRYCTCEKCRLTADRQRVMALQTALRRAQAQDEQRALHMHEVPPANPAATTLLSHHHHVAAPAHVHAHHVHAHHAHGGHHSHHGHVLHHQQAAAAAAAAPSAPASHLGGSSTAASSIHGHAHAHHVHMAAAAAASVAQHQHQSHPHSHHHHHQNHHQHPHQQPATQTALRSPPHSDHGGSVGPATSSSGGGAPSSSNAAAATSSNGSSGGGGGGGGGSSGGGAGGGRSSGTSVITSADHHMTTVPTPAQSLEGSCDSSSPSPSSTSGAAILPISVSVNRKNGANVPLGQDVFLDYCQKLLEKFRYPWELMPLMYVILKDADANIEEASRRIEEGQYVVNEYSRQHNLNIYDGGELRNTTRQCG

Target ORF information:

RefSeq Version	NM_169203.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster doublesex (dsx), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169203.2

ORF Insert Sequence:

ATGGTTTCGG AGGAGAACTG GAATAGCGAC ACGATGTCCG ACTCGGACAT GATCGACTCA 
AAGAACGACG TCTGTGGCGG AGCCTCCAGT TCCAGCGGCA GCTCGATTTC GCCGAGGACA 
CCACCGAACT GCGCCCGCTG CCGCAATCAT GGCCTAAAGA TCACCCTAAA GGGACACAAG 
CGGTACTGCA AGTTCCGCTA CTGCACGTGC GAGAAGTGCC GACTGACGGC GGACCGCCAG 
CGGGTGATGG CTCTGCAAAC GGCCTTGAGG CGAGCCCAGG CGCAGGATGA GCAGCGGGCG 
CTGCACATGC ACGAGGTGCC GCCTGCCAAT CCGGCGGCCA CGACCCTCCT GAGTCATCAC 
CATCATGTCG CAGCTCCCGC TCATGTCCAC GCCCACCATG TGCACGCCCA TCACGCACAC 
GGAGGACACC ACTCGCATCA CGGACATGTC CTGCACCACC AGCAGGCAGC GGCGGCCGCA 
GCAGCAGCTC CGTCGGCTCC AGCCTCCCAT CTGGGTGGCT CCAGCACGGC CGCTTCCAGC 
ATCCACGGTC ACGCCCACGC GCACCACGTC CACATGGCAG CTGCTGCAGC CGCCTCGGTG 
GCTCAGCACC AGCACCAAAG CCACCCACAC TCGCACCACC ACCACCACCA GAACCACCAC 
CAGCATCCGC ATCAGCAACC GGCCACGCAG ACCGCCCTGC GATCTCCGCC GCACAGCGAC 
CATGGTGGCA GTGTGGGTCC GGCCACCAGC AGCTCCGGCG GTGGAGCACC CAGTTCCAGC 
AATGCGGCAG CGGCCACCTC GAGCAACGGA TCCAGCGGCG GTGGAGGAGG AGGCGGAGGG 
GGCAGCTCGG GTGGCGGGGC AGGAGGTGGT AGGTCATCGG GAACATCGGT GATCACTAGC 
GCCGATCACC ACATGACCAC GGTGCCTACG CCCGCCCAAT CGCTGGAGGG GTCCTGCGAC 
TCGTCGTCGC CCTCGCCGTC GTCCACTTCC GGTGCAGCCA TTTTGCCGAT CTCAGTTTCC 
GTCAATCGAA AGAACGGCGC CAATGTGCCC TTGGGCCAAG ACGTTTTCCT AGACTATTGC 
CAAAAGCTAT TAGAAAAATT CCGCTATCCT TGGGAGCTGA TGCCACTCAT GTATGTGATA 
TTAAAGGACG CAGACGCCAA CATTGAAGAG GCTTCCCGGC GAATCGAAGA GGGCCAATAC 
GTTGTGAATG AGTACTCCCG TCAACATAAT TTGAATATCT ATGACGGGGG TGAGCTGCGC 
AACACAACGC GGCAATGTGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dsx ( NM_079548.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524272.4 Drosophila melanogaster doublesex (dsx), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa07972
Clone ID Related Accession (Same CDS sequence)	NM_169203.2 , NM_079548.5 , NM_001300291.1
Accession Version	NM_079548.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	doublesex, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079548.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTCGG AGGAGAACTG GAATAGCGAC ACGATGTCCG ACTCGGACAT GATCGACTCA 
AAGAACGACG TCTGTGGCGG AGCCTCCAGT TCCAGCGGCA GCTCGATTTC GCCGAGGACA 
CCACCGAACT GCGCCCGCTG CCGCAATCAT GGCCTAAAGA TCACCCTAAA GGGACACAAG 
CGGTACTGCA AGTTCCGCTA CTGCACGTGC GAGAAGTGCC GACTGACGGC GGACCGCCAG 
CGGGTGATGG CTCTGCAAAC GGCCTTGAGG CGAGCCCAGG CGCAGGATGA GCAGCGGGCG 
CTGCACATGC ACGAGGTGCC GCCTGCCAAT CCGGCGGCCA CGACCCTCCT GAGTCATCAC 
CATCATGTCG CAGCTCCCGC TCATGTCCAC GCCCACCATG TGCACGCCCA TCACGCACAC 
GGAGGACACC ACTCGCATCA CGGACATGTC CTGCACCACC AGCAGGCAGC GGCGGCCGCA 
GCAGCAGCTC CGTCGGCTCC AGCCTCCCAT CTGGGTGGCT CCAGCACGGC CGCTTCCAGC 
ATCCACGGTC ACGCCCACGC GCACCACGTC CACATGGCAG CTGCTGCAGC CGCCTCGGTG 
GCTCAGCACC AGCACCAAAG CCACCCACAC TCGCACCACC ACCACCACCA GAACCACCAC 
CAGCATCCGC ATCAGCAACC GGCCACGCAG ACCGCCCTGC GATCTCCGCC GCACAGCGAC 
CATGGTGGCA GTGTGGGTCC GGCCACCAGC AGCTCCGGCG GTGGAGCACC CAGTTCCAGC 
AATGCGGCAG CGGCCACCTC GAGCAACGGA TCCAGCGGCG GTGGAGGAGG AGGCGGAGGG 
GGCAGCTCGG GTGGCGGGGC AGGAGGTGGT AGGTCATCGG GAACATCGGT GATCACTAGC 
GCCGATCACC ACATGACCAC GGTGCCTACG CCCGCCCAAT CGCTGGAGGG GTCCTGCGAC 
TCGTCGTCGC CCTCGCCGTC GTCCACTTCC GGTGCAGCCA TTTTGCCGAT CTCAGTTTCC 
GTCAATCGAA AGAACGGCGC CAATGTGCCC TTGGGCCAAG ACGTTTTCCT AGACTATTGC 
CAAAAGCTAT TAGAAAAATT CCGCTATCCT TGGGAGCTGA TGCCACTCAT GTATGTGATA 
TTAAAGGACG CAGACGCCAA CATTGAAGAG GCTTCCCGGC GAATCGAAGA GGGCCAATAC 
GTTGTGAATG AGTACTCCCG TCAACATAAT TTGAATATCT ATGACGGGGG TGAGCTGCGC 
AACACAACGC GGCAATGTGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524272.4
CDS	875..2158
Translation	MVSEENWNSDTMSDSDMIDSKNDVCGGASSSSGSSISPRTPPNCARCRNHGLKITLKGHKRYCKFRYCTCEKCRLTADRQRVMALQTALRRAQAQDEQRALHMHEVPPANPAATTLLSHHHHVAAPAHVHAHHVHAHHAHGGHHSHHGHVLHHQQAAAAAAAAPSAPASHLGGSSTAASSIHGHAHAHHVHMAAAAAASVAQHQHQSHPHSHHHHHQNHHQHPHQQPATQTALRSPPHSDHGGSVGPATSSSGGGAPSSSNAAAATSSNGSSGGGGGGGGGSSGGGAGGGRSSGTSVITSADHHMTTVPTPAQSLEGSCDSSSPSPSSTSGAAILPISVSVNRKNGANVPLGQDVFLDYCQKLLEKFRYPWELMPLMYVILKDADANIEEASRRIEEGQYVVNEYSRQHNLNIYDGGELRNTTRQCG

Target ORF information:

RefSeq Version	NM_079548.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster doublesex (dsx), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079548.5

ORF Insert Sequence:

ATGGTTTCGG AGGAGAACTG GAATAGCGAC ACGATGTCCG ACTCGGACAT GATCGACTCA 
AAGAACGACG TCTGTGGCGG AGCCTCCAGT TCCAGCGGCA GCTCGATTTC GCCGAGGACA 
CCACCGAACT GCGCCCGCTG CCGCAATCAT GGCCTAAAGA TCACCCTAAA GGGACACAAG 
CGGTACTGCA AGTTCCGCTA CTGCACGTGC GAGAAGTGCC GACTGACGGC GGACCGCCAG 
CGGGTGATGG CTCTGCAAAC GGCCTTGAGG CGAGCCCAGG CGCAGGATGA GCAGCGGGCG 
CTGCACATGC ACGAGGTGCC GCCTGCCAAT CCGGCGGCCA CGACCCTCCT GAGTCATCAC 
CATCATGTCG CAGCTCCCGC TCATGTCCAC GCCCACCATG TGCACGCCCA TCACGCACAC 
GGAGGACACC ACTCGCATCA CGGACATGTC CTGCACCACC AGCAGGCAGC GGCGGCCGCA 
GCAGCAGCTC CGTCGGCTCC AGCCTCCCAT CTGGGTGGCT CCAGCACGGC CGCTTCCAGC 
ATCCACGGTC ACGCCCACGC GCACCACGTC CACATGGCAG CTGCTGCAGC CGCCTCGGTG 
GCTCAGCACC AGCACCAAAG CCACCCACAC TCGCACCACC ACCACCACCA GAACCACCAC 
CAGCATCCGC ATCAGCAACC GGCCACGCAG ACCGCCCTGC GATCTCCGCC GCACAGCGAC 
CATGGTGGCA GTGTGGGTCC GGCCACCAGC AGCTCCGGCG GTGGAGCACC CAGTTCCAGC 
AATGCGGCAG CGGCCACCTC GAGCAACGGA TCCAGCGGCG GTGGAGGAGG AGGCGGAGGG 
GGCAGCTCGG GTGGCGGGGC AGGAGGTGGT AGGTCATCGG GAACATCGGT GATCACTAGC 
GCCGATCACC ACATGACCAC GGTGCCTACG CCCGCCCAAT CGCTGGAGGG GTCCTGCGAC 
TCGTCGTCGC CCTCGCCGTC GTCCACTTCC GGTGCAGCCA TTTTGCCGAT CTCAGTTTCC 
GTCAATCGAA AGAACGGCGC CAATGTGCCC TTGGGCCAAG ACGTTTTCCT AGACTATTGC 
CAAAAGCTAT TAGAAAAATT CCGCTATCCT TGGGAGCTGA TGCCACTCAT GTATGTGATA 
TTAAAGGACG CAGACGCCAA CATTGAAGAG GCTTCCCGGC GAATCGAAGA GGGCCAATAC 
GTTGTGAATG AGTACTCCCG TCAACATAAT TTGAATATCT ATGACGGGGG TGAGCTGCGC 
AACACAACGC GGCAATGTGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dsx ( NM_001300291.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287220.1 Drosophila melanogaster doublesex (dsx), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa07972
Clone ID Related Accession (Same CDS sequence)	NM_169203.2 , NM_079548.5 , NM_001300291.1
Accession Version	NM_001300291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	doublesex, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTCGG AGGAGAACTG GAATAGCGAC ACGATGTCCG ACTCGGACAT GATCGACTCA 
AAGAACGACG TCTGTGGCGG AGCCTCCAGT TCCAGCGGCA GCTCGATTTC GCCGAGGACA 
CCACCGAACT GCGCCCGCTG CCGCAATCAT GGCCTAAAGA TCACCCTAAA GGGACACAAG 
CGGTACTGCA AGTTCCGCTA CTGCACGTGC GAGAAGTGCC GACTGACGGC GGACCGCCAG 
CGGGTGATGG CTCTGCAAAC GGCCTTGAGG CGAGCCCAGG CGCAGGATGA GCAGCGGGCG 
CTGCACATGC ACGAGGTGCC GCCTGCCAAT CCGGCGGCCA CGACCCTCCT GAGTCATCAC 
CATCATGTCG CAGCTCCCGC TCATGTCCAC GCCCACCATG TGCACGCCCA TCACGCACAC 
GGAGGACACC ACTCGCATCA CGGACATGTC CTGCACCACC AGCAGGCAGC GGCGGCCGCA 
GCAGCAGCTC CGTCGGCTCC AGCCTCCCAT CTGGGTGGCT CCAGCACGGC CGCTTCCAGC 
ATCCACGGTC ACGCCCACGC GCACCACGTC CACATGGCAG CTGCTGCAGC CGCCTCGGTG 
GCTCAGCACC AGCACCAAAG CCACCCACAC TCGCACCACC ACCACCACCA GAACCACCAC 
CAGCATCCGC ATCAGCAACC GGCCACGCAG ACCGCCCTGC GATCTCCGCC GCACAGCGAC 
CATGGTGGCA GTGTGGGTCC GGCCACCAGC AGCTCCGGCG GTGGAGCACC CAGTTCCAGC 
AATGCGGCAG CGGCCACCTC GAGCAACGGA TCCAGCGGCG GTGGAGGAGG AGGCGGAGGG 
GGCAGCTCGG GTGGCGGGGC AGGAGGTGGT AGGTCATCGG GAACATCGGT GATCACTAGC 
GCCGATCACC ACATGACCAC GGTGCCTACG CCCGCCCAAT CGCTGGAGGG GTCCTGCGAC 
TCGTCGTCGC CCTCGCCGTC GTCCACTTCC GGTGCAGCCA TTTTGCCGAT CTCAGTTTCC 
GTCAATCGAA AGAACGGCGC CAATGTGCCC TTGGGCCAAG ACGTTTTCCT AGACTATTGC 
CAAAAGCTAT TAGAAAAATT CCGCTATCCT TGGGAGCTGA TGCCACTCAT GTATGTGATA 
TTAAAGGACG CAGACGCCAA CATTGAAGAG GCTTCCCGGC GAATCGAAGA GGGCCAATAC 
GTTGTGAATG AGTACTCCCG TCAACATAAT TTGAATATCT ATGACGGGGG TGAGCTGCGC 
AACACAACGC GGCAATGTGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287220.1
CDS	1264..2547
Translation	MVSEENWNSDTMSDSDMIDSKNDVCGGASSSSGSSISPRTPPNCARCRNHGLKITLKGHKRYCKFRYCTCEKCRLTADRQRVMALQTALRRAQAQDEQRALHMHEVPPANPAATTLLSHHHHVAAPAHVHAHHVHAHHAHGGHHSHHGHVLHHQQAAAAAAAAPSAPASHLGGSSTAASSIHGHAHAHHVHMAAAAAASVAQHQHQSHPHSHHHHHQNHHQHPHQQPATQTALRSPPHSDHGGSVGPATSSSGGGAPSSSNAAAATSSNGSSGGGGGGGGGSSGGGAGGGRSSGTSVITSADHHMTTVPTPAQSLEGSCDSSSPSPSSTSGAAILPISVSVNRKNGANVPLGQDVFLDYCQKLLEKFRYPWELMPLMYVILKDADANIEEASRRIEEGQYVVNEYSRQHNLNIYDGGELRNTTRQCG

Target ORF information:

RefSeq Version	NM_001300291.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster doublesex (dsx), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300291.1

ORF Insert Sequence:

ATGGTTTCGG AGGAGAACTG GAATAGCGAC ACGATGTCCG ACTCGGACAT GATCGACTCA 
AAGAACGACG TCTGTGGCGG AGCCTCCAGT TCCAGCGGCA GCTCGATTTC GCCGAGGACA 
CCACCGAACT GCGCCCGCTG CCGCAATCAT GGCCTAAAGA TCACCCTAAA GGGACACAAG 
CGGTACTGCA AGTTCCGCTA CTGCACGTGC GAGAAGTGCC GACTGACGGC GGACCGCCAG 
CGGGTGATGG CTCTGCAAAC GGCCTTGAGG CGAGCCCAGG CGCAGGATGA GCAGCGGGCG 
CTGCACATGC ACGAGGTGCC GCCTGCCAAT CCGGCGGCCA CGACCCTCCT GAGTCATCAC 
CATCATGTCG CAGCTCCCGC TCATGTCCAC GCCCACCATG TGCACGCCCA TCACGCACAC 
GGAGGACACC ACTCGCATCA CGGACATGTC CTGCACCACC AGCAGGCAGC GGCGGCCGCA 
GCAGCAGCTC CGTCGGCTCC AGCCTCCCAT CTGGGTGGCT CCAGCACGGC CGCTTCCAGC 
ATCCACGGTC ACGCCCACGC GCACCACGTC CACATGGCAG CTGCTGCAGC CGCCTCGGTG 
GCTCAGCACC AGCACCAAAG CCACCCACAC TCGCACCACC ACCACCACCA GAACCACCAC 
CAGCATCCGC ATCAGCAACC GGCCACGCAG ACCGCCCTGC GATCTCCGCC GCACAGCGAC 
CATGGTGGCA GTGTGGGTCC GGCCACCAGC AGCTCCGGCG GTGGAGCACC CAGTTCCAGC 
AATGCGGCAG CGGCCACCTC GAGCAACGGA TCCAGCGGCG GTGGAGGAGG AGGCGGAGGG 
GGCAGCTCGG GTGGCGGGGC AGGAGGTGGT AGGTCATCGG GAACATCGGT GATCACTAGC 
GCCGATCACC ACATGACCAC GGTGCCTACG CCCGCCCAAT CGCTGGAGGG GTCCTGCGAC 
TCGTCGTCGC CCTCGCCGTC GTCCACTTCC GGTGCAGCCA TTTTGCCGAT CTCAGTTTCC 
GTCAATCGAA AGAACGGCGC CAATGTGCCC TTGGGCCAAG ACGTTTTCCT AGACTATTGC 
CAAAAGCTAT TAGAAAAATT CCGCTATCCT TGGGAGCTGA TGCCACTCAT GTATGTGATA 
TTAAAGGACG CAGACGCCAA CATTGAAGAG GCTTCCCGGC GAATCGAAGA GGGCCAATAC 
GTTGTGAATG AGTACTCCCG TCAACATAAT TTGAATATCT ATGACGGGGG TGAGCTGCGC 
AACACAACGC GGCAATGTGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
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Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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Combined evidence annotation of transposable elements in genome sequences.
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dsx cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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