wtrw cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following wtrw gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wtrw cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa07964 NM_206459.2
Latest version!
Drosophila melanogaster water witch (wtrw), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa07964 NM_169201.2
Latest version!
Drosophila melanogaster water witch (wtrw), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa07964 NM_169200.2
Latest version!
Drosophila melanogaster water witch (wtrw), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa22060 NM_001300285.1
Latest version!
Drosophila melanogaster water witch (wtrw), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa07964
    Clone ID Related Accession (Same CDS sequence) NM_169201.2 , NM_169200.2 , NM_206459.2
    Accession Version NM_169201.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product water witch, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169201.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGAATC TTGGCTATAA GGAGGGCAGC ACCAAGCCCC GCCGCATGAC ACGTTCGATC 
    AGCGTCGTTA CCAAGACCGA AGTGGAACAG CCGCTCACGG AGAATAATGC AAATAACCGC
    TTCAAATCGA TTCCACCCAA CTTGATGGTG CGATGGCGGA ACAACACTGA CGCCATGATC
    GAAGCCCAGT ACCCGACCGC TGGAGAATTC GAATACATGG AGTGTGGACC GTCGCCGCCG
    GCGGAGAGTG CGCCGAGCAT GTACGACAGC TTCGAGGAGC CGACCAGCGA GCTGAGCGTC
    CAGATATGCA ACGATACGCT GCGGATAAGC CTGATCGACC AGATGAAGAG CGCAGCGGGG
    CGCGTCAAGC TCTTCGAGGA CATCGAGCAG AGCAACGTGG TGGCCGAGGG CATACGGACG
    CACTTTGAGT CCGCCTCAAA GCTGGAGAAG AATCTCTGCT ACCTGTGGGC CGCGTACCTC
    AAGCGCTGGG ATCTGATCGA GAGTCTGCTG GAGGCGGGAG CCGACCTGCA CTTCTGCGAT
    CAGAACGGAA TATCAGCCTT GCACCTTAGC GCCTTCAGTG GCTGCTTGGC CACCTTGGGC
    CTTCTGGTCG CCAAGGGTTT GAATGTCAAT CTGCAGTCCA AGTGCTACAC CCCTCTGCAC
    TGCGCAGCCT TTGGAAATGC TGCGGAGGCA GCAAAGCTAC TGATCAACAA TGGGGCGGAC
    ATATCCAAGG ACACCAGCAA GCCAAACTGC GAGGAGAGCC TGCTGCACTG CGCCGTGCGT
    TCCAATGCCC TGGAGTGTCT GCAGATCTTC ATTGCCGAAG GCGCCGACGT CAACTCGCTC
    AAACCGAATG GCACCAATGC CATTCACCTG GCAGCTGATC TGGGCAATAT TCAGTGTCTG
    GAAGCCTTAC TGAATGCTCC CAATGCAGAT GCCAATGTGC GTATCTGCAT CCGCGAAAAG
    GAGTCCACAG CCTTGCATCT GGCAGCAGAT GAGGGCAACG TGGAGTGTGT GGACTTACTC
    CTGGCCAAAG GTGCTGATGC GAAGTTGAAG AACCATCGCG GTTTCACACC GCTCCACCTG
    GCAGCTAGAA CTTCCAGTCT GGACTGCGTA GAATCGTTGC TGAGGAATGG CAATGCCGAC
    GCCAATGCCG AGGACTTCGA TCACCGTACT CCGCTTCACG CGGCGGTGGG AAAATCGGAG
    AACGCCTATG ACATCATGGA AACGCTCATC CAGTGGGGTG CCAATGTCAA TCATAAGGAC
    ATTTACGGGT TTACAGCTCT TCACCTGGCT GCGTTGGATG GGCTGGTGCA GTGCGTCGAG
    ATGCTGATAT TCCACGGAGC GGATGTGACC ACCAAATCGA AGAAAGGTAC CTCCGCGTTG
    AACGTTATCA CCCGGAAGAC CCCGGCTTCA GTGGCCATGA TCAGACAGAA ACTGGACGCA
    GCTATTACGC TGCACCACTC GCAGGACCCC GTGAACCGTG AGGTGGAATT GGAGCTGGAC
    TTCCGTCAGC TGCTACAGCA CTGTCATCCG CGCGAGATCA GCTACCTAAA CACGTTCGTC
    GACGAGGGTC AGAAGGAGAT ACTGGAGCAC CCGCTCTGCT CCTCGTTTCT GTACATCAAG
    TGGGGCAAGA TCCGCAAGTA CTACATTGGC AGGCTCATCT TCTGCTTCAG CTTCGTGCTT
    TTCCTCACGC TTTACGTGCT GACTGCTTTG GCCCACAACT GCTACAATGG GAGCAAGAAC
    GACAACACGA CCATTCCAGC GCAGGAGTTG TGCCAGAAGC AATCCATCCT GGGAGATATG
    CTCAGAAACA ACCCCTTTGT GATGGAGATG CAATGGTGGG TTCTGGTTGC CATCACCATA
    GTGGAGATTT TTCGAAAGCT ATATGGCATT ACGGGCTACT CCTCATTTCG GCACTACGTA
    ACGCAAGTGG AGAACATAAT GGAGTGGTTC GTGATAACCA GCGTGTTTGT TATATCCTAC
    ATCTACACCA ACAAGACGTA CACGTTTCAA AACCACATAG GCGCCTTCGC CGTGCTGCTC
    GGTTGGACAA ATCTCATGTT GATGATAGGT CAGCTGCCGG TCTTCGATGT CTATGTGGCC
    ATGTACACGA GAGTCCAGGG GGAATTCGCA AAGCTATTTA TGGCCTATTC CTGCATGCTG
    ATTGGTTTTA CCATCAGTTT CTGTGTTATC TTCCCATCGT CGTCGTCGTT TGCCAATCCG
    TTTATGGGCT TCATAACAGT GCTGGTGATG ATGATTGGTG AACAGGATTT ATCGCTGCTC
    ATCAACGATC CCGAAGGCAA GGATCCACCT TTCCTGCTAG AAGTCAGCGC ACAAATCACT
    TTTGTTCTGT TCCTGCTGTT CGTGACCATC ATTCTGATGA ACCTGCTCGT GGGCATTGCA
    GTGCATGATA TCCAAGGATT GAAGAAGACG GCGGGGTTGT CTAAGCTCGT GCGTCAAACG
    AAGCTGATAA GCTACATAGA GTCGGCGCTA TTCAACGGCT ACCTTCCGAC ATGGCTGCGC
    AACTTGCTCC ATTACACCGC GCTGGTCTCT CCCCAAGCTT ATAGAGTGGT TCTGTGCGTC
    AAGCCACTGA ATCCCAGCGA GAAACGCTTG CCCCGGGAAA TCCTAATGAA GGCTTATGAA
    GTAGGAAAAA TGCGCAAGCA TTTCGGTCAT ACTATCTCGT CAAAGAACTC AGCCGAAAAC
    TATTTGTCGT ACAAGAACAA GTATAACAAC AATAATGGCG CCACGACCGG ATATGTACTA
    CCCGATTCGG ACCCGGATGC CGGGCAATTC ACCACACTAA CCACAAAGAT CGATGACAAT
    GCTGATCGGA TCGAGTTCCT CACCCAGGAG ATCCAGGAGC TAAAGCAGGC GCTCATCTCG
    CAGCAACAGC AAGCCAGCAA GGTGATCGAC AAGCTGCTGA TTGTGATCTC CAATCAGCAG
    AAACAGAATC TGCGCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_731194.1
    CDS385..3345
    Translation

    Target ORF information:

    RefSeq Version NM_169201.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster water witch (wtrw), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169201.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGAATC TTGGCTATAA GGAGGGCAGC ACCAAGCCCC GCCGCATGAC ACGTTCGATC 
    AGCGTCGTTA CCAAGACCGA AGTGGAACAG CCGCTCACGG AGAATAATGC AAATAACCGC
    TTCAAATCGA TTCCACCCAA CTTGATGGTG CGATGGCGGA ACAACACTGA CGCCATGATC
    GAAGCCCAGT ACCCGACCGC TGGAGAATTC GAATACATGG AGTGTGGACC GTCGCCGCCG
    GCGGAGAGTG CGCCGAGCAT GTACGACAGC TTCGAGGAGC CGACCAGCGA GCTGAGCGTC
    CAGATATGCA ACGATACGCT GCGGATAAGC CTGATCGACC AGATGAAGAG CGCAGCGGGG
    CGCGTCAAGC TCTTCGAGGA CATCGAGCAG AGCAACGTGG TGGCCGAGGG CATACGGACG
    CACTTTGAGT CCGCCTCAAA GCTGGAGAAG AATCTCTGCT ACCTGTGGGC CGCGTACCTC
    AAGCGCTGGG ATCTGATCGA GAGTCTGCTG GAGGCGGGAG CCGACCTGCA CTTCTGCGAT
    CAGAACGGAA TATCAGCCTT GCACCTTAGC GCCTTCAGTG GCTGCTTGGC CACCTTGGGC
    CTTCTGGTCG CCAAGGGTTT GAATGTCAAT CTGCAGTCCA AGTGCTACAC CCCTCTGCAC
    TGCGCAGCCT TTGGAAATGC TGCGGAGGCA GCAAAGCTAC TGATCAACAA TGGGGCGGAC
    ATATCCAAGG ACACCAGCAA GCCAAACTGC GAGGAGAGCC TGCTGCACTG CGCCGTGCGT
    TCCAATGCCC TGGAGTGTCT GCAGATCTTC ATTGCCGAAG GCGCCGACGT CAACTCGCTC
    AAACCGAATG GCACCAATGC CATTCACCTG GCAGCTGATC TGGGCAATAT TCAGTGTCTG
    GAAGCCTTAC TGAATGCTCC CAATGCAGAT GCCAATGTGC GTATCTGCAT CCGCGAAAAG
    GAGTCCACAG CCTTGCATCT GGCAGCAGAT GAGGGCAACG TGGAGTGTGT GGACTTACTC
    CTGGCCAAAG GTGCTGATGC GAAGTTGAAG AACCATCGCG GTTTCACACC GCTCCACCTG
    GCAGCTAGAA CTTCCAGTCT GGACTGCGTA GAATCGTTGC TGAGGAATGG CAATGCCGAC
    GCCAATGCCG AGGACTTCGA TCACCGTACT CCGCTTCACG CGGCGGTGGG AAAATCGGAG
    AACGCCTATG ACATCATGGA AACGCTCATC CAGTGGGGTG CCAATGTCAA TCATAAGGAC
    ATTTACGGGT TTACAGCTCT TCACCTGGCT GCGTTGGATG GGCTGGTGCA GTGCGTCGAG
    ATGCTGATAT TCCACGGAGC GGATGTGACC ACCAAATCGA AGAAAGGTAC CTCCGCGTTG
    AACGTTATCA CCCGGAAGAC CCCGGCTTCA GTGGCCATGA TCAGACAGAA ACTGGACGCA
    GCTATTACGC TGCACCACTC GCAGGACCCC GTGAACCGTG AGGTGGAATT GGAGCTGGAC
    TTCCGTCAGC TGCTACAGCA CTGTCATCCG CGCGAGATCA GCTACCTAAA CACGTTCGTC
    GACGAGGGTC AGAAGGAGAT ACTGGAGCAC CCGCTCTGCT CCTCGTTTCT GTACATCAAG
    TGGGGCAAGA TCCGCAAGTA CTACATTGGC AGGCTCATCT TCTGCTTCAG CTTCGTGCTT
    TTCCTCACGC TTTACGTGCT GACTGCTTTG GCCCACAACT GCTACAATGG GAGCAAGAAC
    GACAACACGA CCATTCCAGC GCAGGAGTTG TGCCAGAAGC AATCCATCCT GGGAGATATG
    CTCAGAAACA ACCCCTTTGT GATGGAGATG CAATGGTGGG TTCTGGTTGC CATCACCATA
    GTGGAGATTT TTCGAAAGCT ATATGGCATT ACGGGCTACT CCTCATTTCG GCACTACGTA
    ACGCAAGTGG AGAACATAAT GGAGTGGTTC GTGATAACCA GCGTGTTTGT TATATCCTAC
    ATCTACACCA ACAAGACGTA CACGTTTCAA AACCACATAG GCGCCTTCGC CGTGCTGCTC
    GGTTGGACAA ATCTCATGTT GATGATAGGT CAGCTGCCGG TCTTCGATGT CTATGTGGCC
    ATGTACACGA GAGTCCAGGG GGAATTCGCA AAGCTATTTA TGGCCTATTC CTGCATGCTG
    ATTGGTTTTA CCATCAGTTT CTGTGTTATC TTCCCATCGT CGTCGTCGTT TGCCAATCCG
    TTTATGGGCT TCATAACAGT GCTGGTGATG ATGATTGGTG AACAGGATTT ATCGCTGCTC
    ATCAACGATC CCGAAGGCAA GGATCCACCT TTCCTGCTAG AAGTCAGCGC ACAAATCACT
    TTTGTTCTGT TCCTGCTGTT CGTGACCATC ATTCTGATGA ACCTGCTCGT GGGCATTGCA
    GTGCATGATA TCCAAGGATT GAAGAAGACG GCGGGGTTGT CTAAGCTCGT GCGTCAAACG
    AAGCTGATAA GCTACATAGA GTCGGCGCTA TTCAACGGCT ACCTTCCGAC ATGGCTGCGC
    AACTTGCTCC ATTACACCGC GCTGGTCTCT CCCCAAGCTT ATAGAGTGGT TCTGTGCGTC
    AAGCCACTGA ATCCCAGCGA GAAACGCTTG CCCCGGGAAA TCCTAATGAA GGCTTATGAA
    GTAGGAAAAA TGCGCAAGCA TTTCGGTCAT ACTATCTCGT CAAAGAACTC AGCCGAAAAC
    TATTTGTCGT ACAAGAACAA GTATAACAAC AATAATGGCG CCACGACCGG ATATGTACTA
    CCCGATTCGG ACCCGGATGC CGGGCAATTC ACCACACTAA CCACAAAGAT CGATGACAAT
    GCTGATCGGA TCGAGTTCCT CACCCAGGAG ATCCAGGAGC TAAAGCAGGC GCTCATCTCG
    CAGCAACAGC AAGCCAGCAA GGTGATCGAC AAGCTGCTGA TTGTGATCTC CAATCAGCAG
    AAACAGAATC TGCGCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa07964
    Clone ID Related Accession (Same CDS sequence) NM_169201.2 , NM_169200.2 , NM_206459.2
    Accession Version NM_169200.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product water witch, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169200.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGAATC TTGGCTATAA GGAGGGCAGC ACCAAGCCCC GCCGCATGAC ACGTTCGATC 
    AGCGTCGTTA CCAAGACCGA AGTGGAACAG CCGCTCACGG AGAATAATGC AAATAACCGC
    TTCAAATCGA TTCCACCCAA CTTGATGGTG CGATGGCGGA ACAACACTGA CGCCATGATC
    GAAGCCCAGT ACCCGACCGC TGGAGAATTC GAATACATGG AGTGTGGACC GTCGCCGCCG
    GCGGAGAGTG CGCCGAGCAT GTACGACAGC TTCGAGGAGC CGACCAGCGA GCTGAGCGTC
    CAGATATGCA ACGATACGCT GCGGATAAGC CTGATCGACC AGATGAAGAG CGCAGCGGGG
    CGCGTCAAGC TCTTCGAGGA CATCGAGCAG AGCAACGTGG TGGCCGAGGG CATACGGACG
    CACTTTGAGT CCGCCTCAAA GCTGGAGAAG AATCTCTGCT ACCTGTGGGC CGCGTACCTC
    AAGCGCTGGG ATCTGATCGA GAGTCTGCTG GAGGCGGGAG CCGACCTGCA CTTCTGCGAT
    CAGAACGGAA TATCAGCCTT GCACCTTAGC GCCTTCAGTG GCTGCTTGGC CACCTTGGGC
    CTTCTGGTCG CCAAGGGTTT GAATGTCAAT CTGCAGTCCA AGTGCTACAC CCCTCTGCAC
    TGCGCAGCCT TTGGAAATGC TGCGGAGGCA GCAAAGCTAC TGATCAACAA TGGGGCGGAC
    ATATCCAAGG ACACCAGCAA GCCAAACTGC GAGGAGAGCC TGCTGCACTG CGCCGTGCGT
    TCCAATGCCC TGGAGTGTCT GCAGATCTTC ATTGCCGAAG GCGCCGACGT CAACTCGCTC
    AAACCGAATG GCACCAATGC CATTCACCTG GCAGCTGATC TGGGCAATAT TCAGTGTCTG
    GAAGCCTTAC TGAATGCTCC CAATGCAGAT GCCAATGTGC GTATCTGCAT CCGCGAAAAG
    GAGTCCACAG CCTTGCATCT GGCAGCAGAT GAGGGCAACG TGGAGTGTGT GGACTTACTC
    CTGGCCAAAG GTGCTGATGC GAAGTTGAAG AACCATCGCG GTTTCACACC GCTCCACCTG
    GCAGCTAGAA CTTCCAGTCT GGACTGCGTA GAATCGTTGC TGAGGAATGG CAATGCCGAC
    GCCAATGCCG AGGACTTCGA TCACCGTACT CCGCTTCACG CGGCGGTGGG AAAATCGGAG
    AACGCCTATG ACATCATGGA AACGCTCATC CAGTGGGGTG CCAATGTCAA TCATAAGGAC
    ATTTACGGGT TTACAGCTCT TCACCTGGCT GCGTTGGATG GGCTGGTGCA GTGCGTCGAG
    ATGCTGATAT TCCACGGAGC GGATGTGACC ACCAAATCGA AGAAAGGTAC CTCCGCGTTG
    AACGTTATCA CCCGGAAGAC CCCGGCTTCA GTGGCCATGA TCAGACAGAA ACTGGACGCA
    GCTATTACGC TGCACCACTC GCAGGACCCC GTGAACCGTG AGGTGGAATT GGAGCTGGAC
    TTCCGTCAGC TGCTACAGCA CTGTCATCCG CGCGAGATCA GCTACCTAAA CACGTTCGTC
    GACGAGGGTC AGAAGGAGAT ACTGGAGCAC CCGCTCTGCT CCTCGTTTCT GTACATCAAG
    TGGGGCAAGA TCCGCAAGTA CTACATTGGC AGGCTCATCT TCTGCTTCAG CTTCGTGCTT
    TTCCTCACGC TTTACGTGCT GACTGCTTTG GCCCACAACT GCTACAATGG GAGCAAGAAC
    GACAACACGA CCATTCCAGC GCAGGAGTTG TGCCAGAAGC AATCCATCCT GGGAGATATG
    CTCAGAAACA ACCCCTTTGT GATGGAGATG CAATGGTGGG TTCTGGTTGC CATCACCATA
    GTGGAGATTT TTCGAAAGCT ATATGGCATT ACGGGCTACT CCTCATTTCG GCACTACGTA
    ACGCAAGTGG AGAACATAAT GGAGTGGTTC GTGATAACCA GCGTGTTTGT TATATCCTAC
    ATCTACACCA ACAAGACGTA CACGTTTCAA AACCACATAG GCGCCTTCGC CGTGCTGCTC
    GGTTGGACAA ATCTCATGTT GATGATAGGT CAGCTGCCGG TCTTCGATGT CTATGTGGCC
    ATGTACACGA GAGTCCAGGG GGAATTCGCA AAGCTATTTA TGGCCTATTC CTGCATGCTG
    ATTGGTTTTA CCATCAGTTT CTGTGTTATC TTCCCATCGT CGTCGTCGTT TGCCAATCCG
    TTTATGGGCT TCATAACAGT GCTGGTGATG ATGATTGGTG AACAGGATTT ATCGCTGCTC
    ATCAACGATC CCGAAGGCAA GGATCCACCT TTCCTGCTAG AAGTCAGCGC ACAAATCACT
    TTTGTTCTGT TCCTGCTGTT CGTGACCATC ATTCTGATGA ACCTGCTCGT GGGCATTGCA
    GTGCATGATA TCCAAGGATT GAAGAAGACG GCGGGGTTGT CTAAGCTCGT GCGTCAAACG
    AAGCTGATAA GCTACATAGA GTCGGCGCTA TTCAACGGCT ACCTTCCGAC ATGGCTGCGC
    AACTTGCTCC ATTACACCGC GCTGGTCTCT CCCCAAGCTT ATAGAGTGGT TCTGTGCGTC
    AAGCCACTGA ATCCCAGCGA GAAACGCTTG CCCCGGGAAA TCCTAATGAA GGCTTATGAA
    GTAGGAAAAA TGCGCAAGCA TTTCGGTCAT ACTATCTCGT CAAAGAACTC AGCCGAAAAC
    TATTTGTCGT ACAAGAACAA GTATAACAAC AATAATGGCG CCACGACCGG ATATGTACTA
    CCCGATTCGG ACCCGGATGC CGGGCAATTC ACCACACTAA CCACAAAGAT CGATGACAAT
    GCTGATCGGA TCGAGTTCCT CACCCAGGAG ATCCAGGAGC TAAAGCAGGC GCTCATCTCG
    CAGCAACAGC AAGCCAGCAA GGTGATCGAC AAGCTGCTGA TTGTGATCTC CAATCAGCAG
    AAACAGAATC TGCGCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_731193.1
    CDS298..3258
    Translation

    Target ORF information:

    RefSeq Version NM_169200.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster water witch (wtrw), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169200.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGAATC TTGGCTATAA GGAGGGCAGC ACCAAGCCCC GCCGCATGAC ACGTTCGATC 
    AGCGTCGTTA CCAAGACCGA AGTGGAACAG CCGCTCACGG AGAATAATGC AAATAACCGC
    TTCAAATCGA TTCCACCCAA CTTGATGGTG CGATGGCGGA ACAACACTGA CGCCATGATC
    GAAGCCCAGT ACCCGACCGC TGGAGAATTC GAATACATGG AGTGTGGACC GTCGCCGCCG
    GCGGAGAGTG CGCCGAGCAT GTACGACAGC TTCGAGGAGC CGACCAGCGA GCTGAGCGTC
    CAGATATGCA ACGATACGCT GCGGATAAGC CTGATCGACC AGATGAAGAG CGCAGCGGGG
    CGCGTCAAGC TCTTCGAGGA CATCGAGCAG AGCAACGTGG TGGCCGAGGG CATACGGACG
    CACTTTGAGT CCGCCTCAAA GCTGGAGAAG AATCTCTGCT ACCTGTGGGC CGCGTACCTC
    AAGCGCTGGG ATCTGATCGA GAGTCTGCTG GAGGCGGGAG CCGACCTGCA CTTCTGCGAT
    CAGAACGGAA TATCAGCCTT GCACCTTAGC GCCTTCAGTG GCTGCTTGGC CACCTTGGGC
    CTTCTGGTCG CCAAGGGTTT GAATGTCAAT CTGCAGTCCA AGTGCTACAC CCCTCTGCAC
    TGCGCAGCCT TTGGAAATGC TGCGGAGGCA GCAAAGCTAC TGATCAACAA TGGGGCGGAC
    ATATCCAAGG ACACCAGCAA GCCAAACTGC GAGGAGAGCC TGCTGCACTG CGCCGTGCGT
    TCCAATGCCC TGGAGTGTCT GCAGATCTTC ATTGCCGAAG GCGCCGACGT CAACTCGCTC
    AAACCGAATG GCACCAATGC CATTCACCTG GCAGCTGATC TGGGCAATAT TCAGTGTCTG
    GAAGCCTTAC TGAATGCTCC CAATGCAGAT GCCAATGTGC GTATCTGCAT CCGCGAAAAG
    GAGTCCACAG CCTTGCATCT GGCAGCAGAT GAGGGCAACG TGGAGTGTGT GGACTTACTC
    CTGGCCAAAG GTGCTGATGC GAAGTTGAAG AACCATCGCG GTTTCACACC GCTCCACCTG
    GCAGCTAGAA CTTCCAGTCT GGACTGCGTA GAATCGTTGC TGAGGAATGG CAATGCCGAC
    GCCAATGCCG AGGACTTCGA TCACCGTACT CCGCTTCACG CGGCGGTGGG AAAATCGGAG
    AACGCCTATG ACATCATGGA AACGCTCATC CAGTGGGGTG CCAATGTCAA TCATAAGGAC
    ATTTACGGGT TTACAGCTCT TCACCTGGCT GCGTTGGATG GGCTGGTGCA GTGCGTCGAG
    ATGCTGATAT TCCACGGAGC GGATGTGACC ACCAAATCGA AGAAAGGTAC CTCCGCGTTG
    AACGTTATCA CCCGGAAGAC CCCGGCTTCA GTGGCCATGA TCAGACAGAA ACTGGACGCA
    GCTATTACGC TGCACCACTC GCAGGACCCC GTGAACCGTG AGGTGGAATT GGAGCTGGAC
    TTCCGTCAGC TGCTACAGCA CTGTCATCCG CGCGAGATCA GCTACCTAAA CACGTTCGTC
    GACGAGGGTC AGAAGGAGAT ACTGGAGCAC CCGCTCTGCT CCTCGTTTCT GTACATCAAG
    TGGGGCAAGA TCCGCAAGTA CTACATTGGC AGGCTCATCT TCTGCTTCAG CTTCGTGCTT
    TTCCTCACGC TTTACGTGCT GACTGCTTTG GCCCACAACT GCTACAATGG GAGCAAGAAC
    GACAACACGA CCATTCCAGC GCAGGAGTTG TGCCAGAAGC AATCCATCCT GGGAGATATG
    CTCAGAAACA ACCCCTTTGT GATGGAGATG CAATGGTGGG TTCTGGTTGC CATCACCATA
    GTGGAGATTT TTCGAAAGCT ATATGGCATT ACGGGCTACT CCTCATTTCG GCACTACGTA
    ACGCAAGTGG AGAACATAAT GGAGTGGTTC GTGATAACCA GCGTGTTTGT TATATCCTAC
    ATCTACACCA ACAAGACGTA CACGTTTCAA AACCACATAG GCGCCTTCGC CGTGCTGCTC
    GGTTGGACAA ATCTCATGTT GATGATAGGT CAGCTGCCGG TCTTCGATGT CTATGTGGCC
    ATGTACACGA GAGTCCAGGG GGAATTCGCA AAGCTATTTA TGGCCTATTC CTGCATGCTG
    ATTGGTTTTA CCATCAGTTT CTGTGTTATC TTCCCATCGT CGTCGTCGTT TGCCAATCCG
    TTTATGGGCT TCATAACAGT GCTGGTGATG ATGATTGGTG AACAGGATTT ATCGCTGCTC
    ATCAACGATC CCGAAGGCAA GGATCCACCT TTCCTGCTAG AAGTCAGCGC ACAAATCACT
    TTTGTTCTGT TCCTGCTGTT CGTGACCATC ATTCTGATGA ACCTGCTCGT GGGCATTGCA
    GTGCATGATA TCCAAGGATT GAAGAAGACG GCGGGGTTGT CTAAGCTCGT GCGTCAAACG
    AAGCTGATAA GCTACATAGA GTCGGCGCTA TTCAACGGCT ACCTTCCGAC ATGGCTGCGC
    AACTTGCTCC ATTACACCGC GCTGGTCTCT CCCCAAGCTT ATAGAGTGGT TCTGTGCGTC
    AAGCCACTGA ATCCCAGCGA GAAACGCTTG CCCCGGGAAA TCCTAATGAA GGCTTATGAA
    GTAGGAAAAA TGCGCAAGCA TTTCGGTCAT ACTATCTCGT CAAAGAACTC AGCCGAAAAC
    TATTTGTCGT ACAAGAACAA GTATAACAAC AATAATGGCG CCACGACCGG ATATGTACTA
    CCCGATTCGG ACCCGGATGC CGGGCAATTC ACCACACTAA CCACAAAGAT CGATGACAAT
    GCTGATCGGA TCGAGTTCCT CACCCAGGAG ATCCAGGAGC TAAAGCAGGC GCTCATCTCG
    CAGCAACAGC AAGCCAGCAA GGTGATCGAC AAGCTGCTGA TTGTGATCTC CAATCAGCAG
    AAACAGAATC TGCGCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa07964
    Clone ID Related Accession (Same CDS sequence) NM_169201.2 , NM_169200.2 , NM_206459.2
    Accession Version NM_206459.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product water witch, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_206459.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGAATC TTGGCTATAA GGAGGGCAGC ACCAAGCCCC GCCGCATGAC ACGTTCGATC 
    AGCGTCGTTA CCAAGACCGA AGTGGAACAG CCGCTCACGG AGAATAATGC AAATAACCGC
    TTCAAATCGA TTCCACCCAA CTTGATGGTG CGATGGCGGA ACAACACTGA CGCCATGATC
    GAAGCCCAGT ACCCGACCGC TGGAGAATTC GAATACATGG AGTGTGGACC GTCGCCGCCG
    GCGGAGAGTG CGCCGAGCAT GTACGACAGC TTCGAGGAGC CGACCAGCGA GCTGAGCGTC
    CAGATATGCA ACGATACGCT GCGGATAAGC CTGATCGACC AGATGAAGAG CGCAGCGGGG
    CGCGTCAAGC TCTTCGAGGA CATCGAGCAG AGCAACGTGG TGGCCGAGGG CATACGGACG
    CACTTTGAGT CCGCCTCAAA GCTGGAGAAG AATCTCTGCT ACCTGTGGGC CGCGTACCTC
    AAGCGCTGGG ATCTGATCGA GAGTCTGCTG GAGGCGGGAG CCGACCTGCA CTTCTGCGAT
    CAGAACGGAA TATCAGCCTT GCACCTTAGC GCCTTCAGTG GCTGCTTGGC CACCTTGGGC
    CTTCTGGTCG CCAAGGGTTT GAATGTCAAT CTGCAGTCCA AGTGCTACAC CCCTCTGCAC
    TGCGCAGCCT TTGGAAATGC TGCGGAGGCA GCAAAGCTAC TGATCAACAA TGGGGCGGAC
    ATATCCAAGG ACACCAGCAA GCCAAACTGC GAGGAGAGCC TGCTGCACTG CGCCGTGCGT
    TCCAATGCCC TGGAGTGTCT GCAGATCTTC ATTGCCGAAG GCGCCGACGT CAACTCGCTC
    AAACCGAATG GCACCAATGC CATTCACCTG GCAGCTGATC TGGGCAATAT TCAGTGTCTG
    GAAGCCTTAC TGAATGCTCC CAATGCAGAT GCCAATGTGC GTATCTGCAT CCGCGAAAAG
    GAGTCCACAG CCTTGCATCT GGCAGCAGAT GAGGGCAACG TGGAGTGTGT GGACTTACTC
    CTGGCCAAAG GTGCTGATGC GAAGTTGAAG AACCATCGCG GTTTCACACC GCTCCACCTG
    GCAGCTAGAA CTTCCAGTCT GGACTGCGTA GAATCGTTGC TGAGGAATGG CAATGCCGAC
    GCCAATGCCG AGGACTTCGA TCACCGTACT CCGCTTCACG CGGCGGTGGG AAAATCGGAG
    AACGCCTATG ACATCATGGA AACGCTCATC CAGTGGGGTG CCAATGTCAA TCATAAGGAC
    ATTTACGGGT TTACAGCTCT TCACCTGGCT GCGTTGGATG GGCTGGTGCA GTGCGTCGAG
    ATGCTGATAT TCCACGGAGC GGATGTGACC ACCAAATCGA AGAAAGGTAC CTCCGCGTTG
    AACGTTATCA CCCGGAAGAC CCCGGCTTCA GTGGCCATGA TCAGACAGAA ACTGGACGCA
    GCTATTACGC TGCACCACTC GCAGGACCCC GTGAACCGTG AGGTGGAATT GGAGCTGGAC
    TTCCGTCAGC TGCTACAGCA CTGTCATCCG CGCGAGATCA GCTACCTAAA CACGTTCGTC
    GACGAGGGTC AGAAGGAGAT ACTGGAGCAC CCGCTCTGCT CCTCGTTTCT GTACATCAAG
    TGGGGCAAGA TCCGCAAGTA CTACATTGGC AGGCTCATCT TCTGCTTCAG CTTCGTGCTT
    TTCCTCACGC TTTACGTGCT GACTGCTTTG GCCCACAACT GCTACAATGG GAGCAAGAAC
    GACAACACGA CCATTCCAGC GCAGGAGTTG TGCCAGAAGC AATCCATCCT GGGAGATATG
    CTCAGAAACA ACCCCTTTGT GATGGAGATG CAATGGTGGG TTCTGGTTGC CATCACCATA
    GTGGAGATTT TTCGAAAGCT ATATGGCATT ACGGGCTACT CCTCATTTCG GCACTACGTA
    ACGCAAGTGG AGAACATAAT GGAGTGGTTC GTGATAACCA GCGTGTTTGT TATATCCTAC
    ATCTACACCA ACAAGACGTA CACGTTTCAA AACCACATAG GCGCCTTCGC CGTGCTGCTC
    GGTTGGACAA ATCTCATGTT GATGATAGGT CAGCTGCCGG TCTTCGATGT CTATGTGGCC
    ATGTACACGA GAGTCCAGGG GGAATTCGCA AAGCTATTTA TGGCCTATTC CTGCATGCTG
    ATTGGTTTTA CCATCAGTTT CTGTGTTATC TTCCCATCGT CGTCGTCGTT TGCCAATCCG
    TTTATGGGCT TCATAACAGT GCTGGTGATG ATGATTGGTG AACAGGATTT ATCGCTGCTC
    ATCAACGATC CCGAAGGCAA GGATCCACCT TTCCTGCTAG AAGTCAGCGC ACAAATCACT
    TTTGTTCTGT TCCTGCTGTT CGTGACCATC ATTCTGATGA ACCTGCTCGT GGGCATTGCA
    GTGCATGATA TCCAAGGATT GAAGAAGACG GCGGGGTTGT CTAAGCTCGT GCGTCAAACG
    AAGCTGATAA GCTACATAGA GTCGGCGCTA TTCAACGGCT ACCTTCCGAC ATGGCTGCGC
    AACTTGCTCC ATTACACCGC GCTGGTCTCT CCCCAAGCTT ATAGAGTGGT TCTGTGCGTC
    AAGCCACTGA ATCCCAGCGA GAAACGCTTG CCCCGGGAAA TCCTAATGAA GGCTTATGAA
    GTAGGAAAAA TGCGCAAGCA TTTCGGTCAT ACTATCTCGT CAAAGAACTC AGCCGAAAAC
    TATTTGTCGT ACAAGAACAA GTATAACAAC AATAATGGCG CCACGACCGG ATATGTACTA
    CCCGATTCGG ACCCGGATGC CGGGCAATTC ACCACACTAA CCACAAAGAT CGATGACAAT
    GCTGATCGGA TCGAGTTCCT CACCCAGGAG ATCCAGGAGC TAAAGCAGGC GCTCATCTCG
    CAGCAACAGC AAGCCAGCAA GGTGATCGAC AAGCTGCTGA TTGTGATCTC CAATCAGCAG
    AAACAGAATC TGCGCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996181.1
    CDS405..3365
    Translation

    Target ORF information:

    RefSeq Version NM_206459.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster water witch (wtrw), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206459.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGAATC TTGGCTATAA GGAGGGCAGC ACCAAGCCCC GCCGCATGAC ACGTTCGATC 
    AGCGTCGTTA CCAAGACCGA AGTGGAACAG CCGCTCACGG AGAATAATGC AAATAACCGC
    TTCAAATCGA TTCCACCCAA CTTGATGGTG CGATGGCGGA ACAACACTGA CGCCATGATC
    GAAGCCCAGT ACCCGACCGC TGGAGAATTC GAATACATGG AGTGTGGACC GTCGCCGCCG
    GCGGAGAGTG CGCCGAGCAT GTACGACAGC TTCGAGGAGC CGACCAGCGA GCTGAGCGTC
    CAGATATGCA ACGATACGCT GCGGATAAGC CTGATCGACC AGATGAAGAG CGCAGCGGGG
    CGCGTCAAGC TCTTCGAGGA CATCGAGCAG AGCAACGTGG TGGCCGAGGG CATACGGACG
    CACTTTGAGT CCGCCTCAAA GCTGGAGAAG AATCTCTGCT ACCTGTGGGC CGCGTACCTC
    AAGCGCTGGG ATCTGATCGA GAGTCTGCTG GAGGCGGGAG CCGACCTGCA CTTCTGCGAT
    CAGAACGGAA TATCAGCCTT GCACCTTAGC GCCTTCAGTG GCTGCTTGGC CACCTTGGGC
    CTTCTGGTCG CCAAGGGTTT GAATGTCAAT CTGCAGTCCA AGTGCTACAC CCCTCTGCAC
    TGCGCAGCCT TTGGAAATGC TGCGGAGGCA GCAAAGCTAC TGATCAACAA TGGGGCGGAC
    ATATCCAAGG ACACCAGCAA GCCAAACTGC GAGGAGAGCC TGCTGCACTG CGCCGTGCGT
    TCCAATGCCC TGGAGTGTCT GCAGATCTTC ATTGCCGAAG GCGCCGACGT CAACTCGCTC
    AAACCGAATG GCACCAATGC CATTCACCTG GCAGCTGATC TGGGCAATAT TCAGTGTCTG
    GAAGCCTTAC TGAATGCTCC CAATGCAGAT GCCAATGTGC GTATCTGCAT CCGCGAAAAG
    GAGTCCACAG CCTTGCATCT GGCAGCAGAT GAGGGCAACG TGGAGTGTGT GGACTTACTC
    CTGGCCAAAG GTGCTGATGC GAAGTTGAAG AACCATCGCG GTTTCACACC GCTCCACCTG
    GCAGCTAGAA CTTCCAGTCT GGACTGCGTA GAATCGTTGC TGAGGAATGG CAATGCCGAC
    GCCAATGCCG AGGACTTCGA TCACCGTACT CCGCTTCACG CGGCGGTGGG AAAATCGGAG
    AACGCCTATG ACATCATGGA AACGCTCATC CAGTGGGGTG CCAATGTCAA TCATAAGGAC
    ATTTACGGGT TTACAGCTCT TCACCTGGCT GCGTTGGATG GGCTGGTGCA GTGCGTCGAG
    ATGCTGATAT TCCACGGAGC GGATGTGACC ACCAAATCGA AGAAAGGTAC CTCCGCGTTG
    AACGTTATCA CCCGGAAGAC CCCGGCTTCA GTGGCCATGA TCAGACAGAA ACTGGACGCA
    GCTATTACGC TGCACCACTC GCAGGACCCC GTGAACCGTG AGGTGGAATT GGAGCTGGAC
    TTCCGTCAGC TGCTACAGCA CTGTCATCCG CGCGAGATCA GCTACCTAAA CACGTTCGTC
    GACGAGGGTC AGAAGGAGAT ACTGGAGCAC CCGCTCTGCT CCTCGTTTCT GTACATCAAG
    TGGGGCAAGA TCCGCAAGTA CTACATTGGC AGGCTCATCT TCTGCTTCAG CTTCGTGCTT
    TTCCTCACGC TTTACGTGCT GACTGCTTTG GCCCACAACT GCTACAATGG GAGCAAGAAC
    GACAACACGA CCATTCCAGC GCAGGAGTTG TGCCAGAAGC AATCCATCCT GGGAGATATG
    CTCAGAAACA ACCCCTTTGT GATGGAGATG CAATGGTGGG TTCTGGTTGC CATCACCATA
    GTGGAGATTT TTCGAAAGCT ATATGGCATT ACGGGCTACT CCTCATTTCG GCACTACGTA
    ACGCAAGTGG AGAACATAAT GGAGTGGTTC GTGATAACCA GCGTGTTTGT TATATCCTAC
    ATCTACACCA ACAAGACGTA CACGTTTCAA AACCACATAG GCGCCTTCGC CGTGCTGCTC
    GGTTGGACAA ATCTCATGTT GATGATAGGT CAGCTGCCGG TCTTCGATGT CTATGTGGCC
    ATGTACACGA GAGTCCAGGG GGAATTCGCA AAGCTATTTA TGGCCTATTC CTGCATGCTG
    ATTGGTTTTA CCATCAGTTT CTGTGTTATC TTCCCATCGT CGTCGTCGTT TGCCAATCCG
    TTTATGGGCT TCATAACAGT GCTGGTGATG ATGATTGGTG AACAGGATTT ATCGCTGCTC
    ATCAACGATC CCGAAGGCAA GGATCCACCT TTCCTGCTAG AAGTCAGCGC ACAAATCACT
    TTTGTTCTGT TCCTGCTGTT CGTGACCATC ATTCTGATGA ACCTGCTCGT GGGCATTGCA
    GTGCATGATA TCCAAGGATT GAAGAAGACG GCGGGGTTGT CTAAGCTCGT GCGTCAAACG
    AAGCTGATAA GCTACATAGA GTCGGCGCTA TTCAACGGCT ACCTTCCGAC ATGGCTGCGC
    AACTTGCTCC ATTACACCGC GCTGGTCTCT CCCCAAGCTT ATAGAGTGGT TCTGTGCGTC
    AAGCCACTGA ATCCCAGCGA GAAACGCTTG CCCCGGGAAA TCCTAATGAA GGCTTATGAA
    GTAGGAAAAA TGCGCAAGCA TTTCGGTCAT ACTATCTCGT CAAAGAACTC AGCCGAAAAC
    TATTTGTCGT ACAAGAACAA GTATAACAAC AATAATGGCG CCACGACCGG ATATGTACTA
    CCCGATTCGG ACCCGGATGC CGGGCAATTC ACCACACTAA CCACAAAGAT CGATGACAAT
    GCTGATCGGA TCGAGTTCCT CACCCAGGAG ATCCAGGAGC TAAAGCAGGC GCTCATCTCG
    CAGCAACAGC AAGCCAGCAA GGTGATCGAC AAGCTGCTGA TTGTGATCTC CAATCAGCAG
    AAACAGAATC TGCGCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa22060
    Clone ID Related Accession (Same CDS sequence) NM_001300285.1
    Accession Version NM_001300285.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2910bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product water witch, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAATTAG AAATTGAAAA CAGCTCACTG TCGAATTGGC ATCATCCGTG GATATGGCTG 
    CAAACGGTCT TCAAATCGAT TCCACCCAAC TTGATGGTGC GATGGCGGAA CAACACTGAC
    GCCATGATCG AAGCCCAGTA CCCGACCGCT GGAGAATTCG AATACATGGA GTGTGGACCG
    TCGCCGCCGG CGGAGAGTGC GCCGAGCATG TACGACAGCT TCGAGGAGCC GACCAGCGAG
    CTGAGCGTCC AGATATGCAA CGATACGCTG CGGATAAGCC TGATCGACCA GATGAAGAGC
    GCAGCGGGGC GCGTCAAGCT CTTCGAGGAC ATCGAGCAGA GCAACGTGGT GGCCGAGGGC
    ATACGGACGC ACTTTGAGTC CGCCTCAAAG CTGGAGAAGA ATCTCTGCTA CCTGTGGGCC
    GCGTACCTCA AGCGCTGGGA TCTGATCGAG AGTCTGCTGG AGGCGGGAGC CGACCTGCAC
    TTCTGCGATC AGAACGGAAT ATCAGCCTTG CACCTTAGCG CCTTCAGTGG CTGCTTGGCC
    ACCTTGGGCC TTCTGGTCGC CAAGGGTTTG AATGTCAATC TGCAGTCCAA GTGCTACACC
    CCTCTGCACT GCGCAGCCTT TGGAAATGCT GCGGAGGCAG CAAAGCTACT GATCAACAAT
    GGGGCGGACA TATCCAAGGA CACCAGCAAG CCAAACTGCG AGGAGAGCCT GCTGCACTGC
    GCCGTGCGTT CCAATGCCCT GGAGTGTCTG CAGATCTTCA TTGCCGAAGG CGCCGACGTC
    AACTCGCTCA AACCGAATGG CACCAATGCC ATTCACCTGG CAGCTGATCT GGGCAATATT
    CAGTGTCTGG AAGCCTTACT GAATGCTCCC AATGCAGATG CCAATGTGCG TATCTGCATC
    CGCGAAAAGG AGTCCACAGC CTTGCATCTG GCAGCAGATG AGGGCAACGT GGAGTGTGTG
    GACTTACTCC TGGCCAAAGG TGCTGATGCG AAGTTGAAGA ACCATCGCGG TTTCACACCG
    CTCCACCTGG CAGCTAGAAC TTCCAGTCTG GACTGCGTAG AATCGTTGCT GAGGAATGGC
    AATGCCGACG CCAATGCCGA GGACTTCGAT CACCGTACTC CGCTTCACGC GGCGGTGGGA
    AAATCGGAGA ACGCCTATGA CATCATGGAA ACGCTCATCC AGTGGGGTGC CAATGTCAAT
    CATAAGGACA TTTACGGGTT TACAGCTCTT CACCTGGCTG CGTTGGATGG GCTGGTGCAG
    TGCGTCGAGA TGCTGATATT CCACGGAGCG GATGTGACCA CCAAATCGAA GAAAGGTACC
    TCCGCGTTGA ACGTTATCAC CCGGAAGACC CCGGCTTCAG TGGCCATGAT CAGACAGAAA
    CTGGACGCAG CTATTACGCT GCACCACTCG CAGGACCCCG TGAACCGTGA GGTGGAATTG
    GAGCTGGACT TCCGTCAGCT GCTACAGCAC TGTCATCCGC GCGAGATCAG CTACCTAAAC
    ACGTTCGTCG ACGAGGGTCA GAAGGAGATA CTGGAGCACC CGCTCTGCTC CTCGTTTCTG
    TACATCAAGT GGGGCAAGAT CCGCAAGTAC TACATTGGCA GGCTCATCTT CTGCTTCAGC
    TTCGTGCTTT TCCTCACGCT TTACGTGCTG ACTGCTTTGG CCCACAACTG CTACAATGGG
    AGCAAGAACG ACAACACGAC CATTCCAGCG CAGGAGTTGT GCCAGAAGCA ATCCATCCTG
    GGAGATATGC TCAGAAACAA CCCCTTTGTG ATGGAGATGC AATGGTGGGT TCTGGTTGCC
    ATCACCATAG TGGAGATTTT TCGAAAGCTA TATGGCATTA CGGGCTACTC CTCATTTCGG
    CACTACGTAA CGCAAGTGGA GAACATAATG GAGTGGTTCG TGATAACCAG CGTGTTTGTT
    ATATCCTACA TCTACACCAA CAAGACGTAC ACGTTTCAAA ACCACATAGG CGCCTTCGCC
    GTGCTGCTCG GTTGGACAAA TCTCATGTTG ATGATAGGTC AGCTGCCGGT CTTCGATGTC
    TATGTGGCCA TGTACACGAG AGTCCAGGGG GAATTCGCAA AGCTATTTAT GGCCTATTCC
    TGCATGCTGA TTGGTTTTAC CATCAGTTTC TGTGTTATCT TCCCATCGTC GTCGTCGTTT
    GCCAATCCGT TTATGGGCTT CATAACAGTG CTGGTGATGA TGATTGGTGA ACAGGATTTA
    TCGCTGCTCA TCAACGATCC CGAAGGCAAG GATCCACCTT TCCTGCTAGA AGTCAGCGCA
    CAAATCACTT TTGTTCTGTT CCTGCTGTTC GTGACCATCA TTCTGATGAA CCTGCTCGTG
    GGCATTGCAG TGCATGATAT CCAAGGATTG AAGAAGACGG CGGGGTTGTC TAAGCTCGTG
    CGTCAAACGA AGCTGATAAG CTACATAGAG TCGGCGCTAT TCAACGGCTA CCTTCCGACA
    TGGCTGCGCA ACTTGCTCCA TTACACCGCG CTGGTCTCTC CCCAAGCTTA TAGAGTGGTT
    CTGTGCGTCA AGCCACTGAA TCCCAGCGAG AAACGCTTGC CCCGGGAAAT CCTAATGAAG
    GCTTATGAAG TAGGAAAAAT GCGCAAGCAT TTCGGTCATA CTATCTCGTC AAAGAACTCA
    GCCGAAAACT ATTTGTCGTA CAAGAACAAG TATAACAACA ATAATGGCGC CACGACCGGA
    TATGTACTAC CCGATTCGGA CCCGGATGCC GGGCAATTCA CCACACTAAC CACAAAGATC
    GATGACAATG CTGATCGGAT CGAGTTCCTC ACCCAGGAGA TCCAGGAGCT AAAGCAGGCG
    CTCATCTCGC AGCAACAGCA AGCCAGCAAG GTGATCGACA AGCTGCTGAT TGTGATCTCC
    AATCAGCAGA AACAGAATCT GCGCAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287214.1
    CDS455..3364
    Translation

    Target ORF information:

    RefSeq Version NM_001300285.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster water witch (wtrw), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300285.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAATTAG AAATTGAAAA CAGCTCACTG TCGAATTGGC ATCATCCGTG GATATGGCTG 
    CAAACGGTCT TCAAATCGAT TCCACCCAAC TTGATGGTGC GATGGCGGAA CAACACTGAC
    GCCATGATCG AAGCCCAGTA CCCGACCGCT GGAGAATTCG AATACATGGA GTGTGGACCG
    TCGCCGCCGG CGGAGAGTGC GCCGAGCATG TACGACAGCT TCGAGGAGCC GACCAGCGAG
    CTGAGCGTCC AGATATGCAA CGATACGCTG CGGATAAGCC TGATCGACCA GATGAAGAGC
    GCAGCGGGGC GCGTCAAGCT CTTCGAGGAC ATCGAGCAGA GCAACGTGGT GGCCGAGGGC
    ATACGGACGC ACTTTGAGTC CGCCTCAAAG CTGGAGAAGA ATCTCTGCTA CCTGTGGGCC
    GCGTACCTCA AGCGCTGGGA TCTGATCGAG AGTCTGCTGG AGGCGGGAGC CGACCTGCAC
    TTCTGCGATC AGAACGGAAT ATCAGCCTTG CACCTTAGCG CCTTCAGTGG CTGCTTGGCC
    ACCTTGGGCC TTCTGGTCGC CAAGGGTTTG AATGTCAATC TGCAGTCCAA GTGCTACACC
    CCTCTGCACT GCGCAGCCTT TGGAAATGCT GCGGAGGCAG CAAAGCTACT GATCAACAAT
    GGGGCGGACA TATCCAAGGA CACCAGCAAG CCAAACTGCG AGGAGAGCCT GCTGCACTGC
    GCCGTGCGTT CCAATGCCCT GGAGTGTCTG CAGATCTTCA TTGCCGAAGG CGCCGACGTC
    AACTCGCTCA AACCGAATGG CACCAATGCC ATTCACCTGG CAGCTGATCT GGGCAATATT
    CAGTGTCTGG AAGCCTTACT GAATGCTCCC AATGCAGATG CCAATGTGCG TATCTGCATC
    CGCGAAAAGG AGTCCACAGC CTTGCATCTG GCAGCAGATG AGGGCAACGT GGAGTGTGTG
    GACTTACTCC TGGCCAAAGG TGCTGATGCG AAGTTGAAGA ACCATCGCGG TTTCACACCG
    CTCCACCTGG CAGCTAGAAC TTCCAGTCTG GACTGCGTAG AATCGTTGCT GAGGAATGGC
    AATGCCGACG CCAATGCCGA GGACTTCGAT CACCGTACTC CGCTTCACGC GGCGGTGGGA
    AAATCGGAGA ACGCCTATGA CATCATGGAA ACGCTCATCC AGTGGGGTGC CAATGTCAAT
    CATAAGGACA TTTACGGGTT TACAGCTCTT CACCTGGCTG CGTTGGATGG GCTGGTGCAG
    TGCGTCGAGA TGCTGATATT CCACGGAGCG GATGTGACCA CCAAATCGAA GAAAGGTACC
    TCCGCGTTGA ACGTTATCAC CCGGAAGACC CCGGCTTCAG TGGCCATGAT CAGACAGAAA
    CTGGACGCAG CTATTACGCT GCACCACTCG CAGGACCCCG TGAACCGTGA GGTGGAATTG
    GAGCTGGACT TCCGTCAGCT GCTACAGCAC TGTCATCCGC GCGAGATCAG CTACCTAAAC
    ACGTTCGTCG ACGAGGGTCA GAAGGAGATA CTGGAGCACC CGCTCTGCTC CTCGTTTCTG
    TACATCAAGT GGGGCAAGAT CCGCAAGTAC TACATTGGCA GGCTCATCTT CTGCTTCAGC
    TTCGTGCTTT TCCTCACGCT TTACGTGCTG ACTGCTTTGG CCCACAACTG CTACAATGGG
    AGCAAGAACG ACAACACGAC CATTCCAGCG CAGGAGTTGT GCCAGAAGCA ATCCATCCTG
    GGAGATATGC TCAGAAACAA CCCCTTTGTG ATGGAGATGC AATGGTGGGT TCTGGTTGCC
    ATCACCATAG TGGAGATTTT TCGAAAGCTA TATGGCATTA CGGGCTACTC CTCATTTCGG
    CACTACGTAA CGCAAGTGGA GAACATAATG GAGTGGTTCG TGATAACCAG CGTGTTTGTT
    ATATCCTACA TCTACACCAA CAAGACGTAC ACGTTTCAAA ACCACATAGG CGCCTTCGCC
    GTGCTGCTCG GTTGGACAAA TCTCATGTTG ATGATAGGTC AGCTGCCGGT CTTCGATGTC
    TATGTGGCCA TGTACACGAG AGTCCAGGGG GAATTCGCAA AGCTATTTAT GGCCTATTCC
    TGCATGCTGA TTGGTTTTAC CATCAGTTTC TGTGTTATCT TCCCATCGTC GTCGTCGTTT
    GCCAATCCGT TTATGGGCTT CATAACAGTG CTGGTGATGA TGATTGGTGA ACAGGATTTA
    TCGCTGCTCA TCAACGATCC CGAAGGCAAG GATCCACCTT TCCTGCTAGA AGTCAGCGCA
    CAAATCACTT TTGTTCTGTT CCTGCTGTTC GTGACCATCA TTCTGATGAA CCTGCTCGTG
    GGCATTGCAG TGCATGATAT CCAAGGATTG AAGAAGACGG CGGGGTTGTC TAAGCTCGTG
    CGTCAAACGA AGCTGATAAG CTACATAGAG TCGGCGCTAT TCAACGGCTA CCTTCCGACA
    TGGCTGCGCA ACTTGCTCCA TTACACCGCG CTGGTCTCTC CCCAAGCTTA TAGAGTGGTT
    CTGTGCGTCA AGCCACTGAA TCCCAGCGAG AAACGCTTGC CCCGGGAAAT CCTAATGAAG
    GCTTATGAAG TAGGAAAAAT GCGCAAGCAT TTCGGTCATA CTATCTCGTC AAAGAACTCA
    GCCGAAAACT ATTTGTCGTA CAAGAACAAG TATAACAACA ATAATGGCGC CACGACCGGA
    TATGTACTAC CCGATTCGGA CCCGGATGCC GGGCAATTCA CCACACTAAC CACAAAGATC
    GATGACAATG CTGATCGGAT CGAGTTCCTC ACCCAGGAGA TCCAGGAGCT AAAGCAGGCG
    CTCATCTCGC AGCAACAGCA AGCCAGCAAG GTGATCGACA AGCTGCTGAT TGTGATCTCC
    AATCAGCAGA AACAGAATCT GCGCAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.