wtrw cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wtrw
Entrez Gene ID	40931
Full Name	water witch
Synonyms	153412_at,CG10409,CG17817,CG31284,CT29234,Dmel\CG31284,Wtrw,dTRPA3,trpA3
General protein information	Preferred Names water witch Names CG31284 gene product from transcript CG31284-RB CG31284-PA CG31284-PB CG31284-PC CG31284-PD waterwitch wtrw-PA wtrw-PB wtrw-PC wtrw-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84E4-84E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_169201.2	NP_731194.1	water witch, isoform B
NM_169200.2	NP_731193.1	water witch, isoform A
NM_206459.2	NP_996181.1	water witch, isoform C
NM_001300285.1	NP_001287214.1	water witch, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	wtrw	NP_731194.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000361	XP_310750.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identification of two Drosophila melanogaster transient receptor potential channels needed for sensing humidity: water witch (wtrw), which is required to detect moist air, and nanchung (nan), which is involved in detecting dry air
Title: Drosophila hygrosensation requires the TRP channels water witch and nanchung.

The following wtrw gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wtrw cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07964	NM_206459.2 Latest version!	Drosophila melanogaster water witch (wtrw), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa07964	NM_169201.2 Latest version!	Drosophila melanogaster water witch (wtrw), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa07964	NM_169200.2 Latest version!	Drosophila melanogaster water witch (wtrw), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa22060	NM_001300285.1 Latest version!	Drosophila melanogaster water witch (wtrw), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wtrw ( NM_169201.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731194.1 Drosophila melanogaster water witch (wtrw), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa07964
Clone ID Related Accession (Same CDS sequence)	NM_169201.2 , NM_169200.2 , NM_206459.2
Accession Version	NM_169201.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	water witch, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169201.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAATC TTGGCTATAA GGAGGGCAGC ACCAAGCCCC GCCGCATGAC ACGTTCGATC 
AGCGTCGTTA CCAAGACCGA AGTGGAACAG CCGCTCACGG AGAATAATGC AAATAACCGC 
TTCAAATCGA TTCCACCCAA CTTGATGGTG CGATGGCGGA ACAACACTGA CGCCATGATC 
GAAGCCCAGT ACCCGACCGC TGGAGAATTC GAATACATGG AGTGTGGACC GTCGCCGCCG 
GCGGAGAGTG CGCCGAGCAT GTACGACAGC TTCGAGGAGC CGACCAGCGA GCTGAGCGTC 
CAGATATGCA ACGATACGCT GCGGATAAGC CTGATCGACC AGATGAAGAG CGCAGCGGGG 
CGCGTCAAGC TCTTCGAGGA CATCGAGCAG AGCAACGTGG TGGCCGAGGG CATACGGACG 
CACTTTGAGT CCGCCTCAAA GCTGGAGAAG AATCTCTGCT ACCTGTGGGC CGCGTACCTC 
AAGCGCTGGG ATCTGATCGA GAGTCTGCTG GAGGCGGGAG CCGACCTGCA CTTCTGCGAT 
CAGAACGGAA TATCAGCCTT GCACCTTAGC GCCTTCAGTG GCTGCTTGGC CACCTTGGGC 
CTTCTGGTCG CCAAGGGTTT GAATGTCAAT CTGCAGTCCA AGTGCTACAC CCCTCTGCAC 
TGCGCAGCCT TTGGAAATGC TGCGGAGGCA GCAAAGCTAC TGATCAACAA TGGGGCGGAC 
ATATCCAAGG ACACCAGCAA GCCAAACTGC GAGGAGAGCC TGCTGCACTG CGCCGTGCGT 
TCCAATGCCC TGGAGTGTCT GCAGATCTTC ATTGCCGAAG GCGCCGACGT CAACTCGCTC 
AAACCGAATG GCACCAATGC CATTCACCTG GCAGCTGATC TGGGCAATAT TCAGTGTCTG 
GAAGCCTTAC TGAATGCTCC CAATGCAGAT GCCAATGTGC GTATCTGCAT CCGCGAAAAG 
GAGTCCACAG CCTTGCATCT GGCAGCAGAT GAGGGCAACG TGGAGTGTGT GGACTTACTC 
CTGGCCAAAG GTGCTGATGC GAAGTTGAAG AACCATCGCG GTTTCACACC GCTCCACCTG 
GCAGCTAGAA CTTCCAGTCT GGACTGCGTA GAATCGTTGC TGAGGAATGG CAATGCCGAC 
GCCAATGCCG AGGACTTCGA TCACCGTACT CCGCTTCACG CGGCGGTGGG AAAATCGGAG 
AACGCCTATG ACATCATGGA AACGCTCATC CAGTGGGGTG CCAATGTCAA TCATAAGGAC 
ATTTACGGGT TTACAGCTCT TCACCTGGCT GCGTTGGATG GGCTGGTGCA GTGCGTCGAG 
ATGCTGATAT TCCACGGAGC GGATGTGACC ACCAAATCGA AGAAAGGTAC CTCCGCGTTG 
AACGTTATCA CCCGGAAGAC CCCGGCTTCA GTGGCCATGA TCAGACAGAA ACTGGACGCA 
GCTATTACGC TGCACCACTC GCAGGACCCC GTGAACCGTG AGGTGGAATT GGAGCTGGAC 
TTCCGTCAGC TGCTACAGCA CTGTCATCCG CGCGAGATCA GCTACCTAAA CACGTTCGTC 
GACGAGGGTC AGAAGGAGAT ACTGGAGCAC CCGCTCTGCT CCTCGTTTCT GTACATCAAG 
TGGGGCAAGA TCCGCAAGTA CTACATTGGC AGGCTCATCT TCTGCTTCAG CTTCGTGCTT 
TTCCTCACGC TTTACGTGCT GACTGCTTTG GCCCACAACT GCTACAATGG GAGCAAGAAC 
GACAACACGA CCATTCCAGC GCAGGAGTTG TGCCAGAAGC AATCCATCCT GGGAGATATG 
CTCAGAAACA ACCCCTTTGT GATGGAGATG CAATGGTGGG TTCTGGTTGC CATCACCATA 
GTGGAGATTT TTCGAAAGCT ATATGGCATT ACGGGCTACT CCTCATTTCG GCACTACGTA 
ACGCAAGTGG AGAACATAAT GGAGTGGTTC GTGATAACCA GCGTGTTTGT TATATCCTAC 
ATCTACACCA ACAAGACGTA CACGTTTCAA AACCACATAG GCGCCTTCGC CGTGCTGCTC 
GGTTGGACAA ATCTCATGTT GATGATAGGT CAGCTGCCGG TCTTCGATGT CTATGTGGCC 
ATGTACACGA GAGTCCAGGG GGAATTCGCA AAGCTATTTA TGGCCTATTC CTGCATGCTG 
ATTGGTTTTA CCATCAGTTT CTGTGTTATC TTCCCATCGT CGTCGTCGTT TGCCAATCCG 
TTTATGGGCT TCATAACAGT GCTGGTGATG ATGATTGGTG AACAGGATTT ATCGCTGCTC 
ATCAACGATC CCGAAGGCAA GGATCCACCT TTCCTGCTAG AAGTCAGCGC ACAAATCACT 
TTTGTTCTGT TCCTGCTGTT CGTGACCATC ATTCTGATGA ACCTGCTCGT GGGCATTGCA 
GTGCATGATA TCCAAGGATT GAAGAAGACG GCGGGGTTGT CTAAGCTCGT GCGTCAAACG 
AAGCTGATAA GCTACATAGA GTCGGCGCTA TTCAACGGCT ACCTTCCGAC ATGGCTGCGC 
AACTTGCTCC ATTACACCGC GCTGGTCTCT CCCCAAGCTT ATAGAGTGGT TCTGTGCGTC 
AAGCCACTGA ATCCCAGCGA GAAACGCTTG CCCCGGGAAA TCCTAATGAA GGCTTATGAA 
GTAGGAAAAA TGCGCAAGCA TTTCGGTCAT ACTATCTCGT CAAAGAACTC AGCCGAAAAC 
TATTTGTCGT ACAAGAACAA GTATAACAAC AATAATGGCG CCACGACCGG ATATGTACTA 
CCCGATTCGG ACCCGGATGC CGGGCAATTC ACCACACTAA CCACAAAGAT CGATGACAAT 
GCTGATCGGA TCGAGTTCCT CACCCAGGAG ATCCAGGAGC TAAAGCAGGC GCTCATCTCG 
CAGCAACAGC AAGCCAGCAA GGTGATCGAC AAGCTGCTGA TTGTGATCTC CAATCAGCAG 
AAACAGAATC TGCGCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731194.1
CDS	385..3345
Translation	MENLGYKEGSTKPRRMTRSISVVTKTEVEQPLTENNANNRFKSIPPNLMVRWRNNTDAMIEAQYPTAGEFEYMECGPSPPAESAPSMYDSFEEPTSELSVQICNDTLRISLIDQMKSAAGRVKLFEDIEQSNVVAEGIRTHFESASKLEKNLCYLWAAYLKRWDLIESLLEAGADLHFCDQNGISALHLSAFSGCLATLGLLVAKGLNVNLQSKCYTPLHCAAFGNAAEAAKLLINNGADISKDTSKPNCEESLLHCAVRSNALECLQIFIAEGADVNSLKPNGTNAIHLAADLGNIQCLEALLNAPNADANVRICIREKESTALHLAADEGNVECVDLLLAKGADAKLKNHRGFTPLHLAARTSSLDCVESLLRNGNADANAEDFDHRTPLHAAVGKSENAYDIMETLIQWGANVNHKDIYGFTALHLAALDGLVQCVEMLIFHGADVTTKSKKGTSALNVITRKTPASVAMIRQKLDAAITLHHSQDPVNREVELELDFRQLLQHCHPREISYLNTFVDEGQKEILEHPLCSSFLYIKWGKIRKYYIGRLIFCFSFVLFLTLYVLTALAHNCYNGSKNDNTTIPAQELCQKQSILGDMLRNNPFVMEMQWWVLVAITIVEIFRKLYGITGYSSFRHYVTQVENIMEWFVITSVFVISYIYTNKTYTFQNHIGAFAVLLGWTNLMLMIGQLPVFDVYVAMYTRVQGEFAKLFMAYSCMLIGFTISFCVIFPSSSSFANPFMGFITVLVMMIGEQDLSLLINDPEGKDPPFLLEVSAQITFVLFLLFVTIILMNLLVGIAVHDIQGLKKTAGLSKLVRQTKLISYIESALFNGYLPTWLRNLLHYTALVSPQAYRVVLCVKPLNPSEKRLPREILMKAYEVGKMRKHFGHTISSKNSAENYLSYKNKYNNNNGATTGYVLPDSDPDAGQFTTLTTKIDDNADRIEFLTQEIQELKQALISQQQQASKVIDKLLIVISNQQKQNLRK

Target ORF information:

RefSeq Version	NM_169201.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster water witch (wtrw), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169201.2

ORF Insert Sequence:

ATGGAGAATC TTGGCTATAA GGAGGGCAGC ACCAAGCCCC GCCGCATGAC ACGTTCGATC 
AGCGTCGTTA CCAAGACCGA AGTGGAACAG CCGCTCACGG AGAATAATGC AAATAACCGC 
TTCAAATCGA TTCCACCCAA CTTGATGGTG CGATGGCGGA ACAACACTGA CGCCATGATC 
GAAGCCCAGT ACCCGACCGC TGGAGAATTC GAATACATGG AGTGTGGACC GTCGCCGCCG 
GCGGAGAGTG CGCCGAGCAT GTACGACAGC TTCGAGGAGC CGACCAGCGA GCTGAGCGTC 
CAGATATGCA ACGATACGCT GCGGATAAGC CTGATCGACC AGATGAAGAG CGCAGCGGGG 
CGCGTCAAGC TCTTCGAGGA CATCGAGCAG AGCAACGTGG TGGCCGAGGG CATACGGACG 
CACTTTGAGT CCGCCTCAAA GCTGGAGAAG AATCTCTGCT ACCTGTGGGC CGCGTACCTC 
AAGCGCTGGG ATCTGATCGA GAGTCTGCTG GAGGCGGGAG CCGACCTGCA CTTCTGCGAT 
CAGAACGGAA TATCAGCCTT GCACCTTAGC GCCTTCAGTG GCTGCTTGGC CACCTTGGGC 
CTTCTGGTCG CCAAGGGTTT GAATGTCAAT CTGCAGTCCA AGTGCTACAC CCCTCTGCAC 
TGCGCAGCCT TTGGAAATGC TGCGGAGGCA GCAAAGCTAC TGATCAACAA TGGGGCGGAC 
ATATCCAAGG ACACCAGCAA GCCAAACTGC GAGGAGAGCC TGCTGCACTG CGCCGTGCGT 
TCCAATGCCC TGGAGTGTCT GCAGATCTTC ATTGCCGAAG GCGCCGACGT CAACTCGCTC 
AAACCGAATG GCACCAATGC CATTCACCTG GCAGCTGATC TGGGCAATAT TCAGTGTCTG 
GAAGCCTTAC TGAATGCTCC CAATGCAGAT GCCAATGTGC GTATCTGCAT CCGCGAAAAG 
GAGTCCACAG CCTTGCATCT GGCAGCAGAT GAGGGCAACG TGGAGTGTGT GGACTTACTC 
CTGGCCAAAG GTGCTGATGC GAAGTTGAAG AACCATCGCG GTTTCACACC GCTCCACCTG 
GCAGCTAGAA CTTCCAGTCT GGACTGCGTA GAATCGTTGC TGAGGAATGG CAATGCCGAC 
GCCAATGCCG AGGACTTCGA TCACCGTACT CCGCTTCACG CGGCGGTGGG AAAATCGGAG 
AACGCCTATG ACATCATGGA AACGCTCATC CAGTGGGGTG CCAATGTCAA TCATAAGGAC 
ATTTACGGGT TTACAGCTCT TCACCTGGCT GCGTTGGATG GGCTGGTGCA GTGCGTCGAG 
ATGCTGATAT TCCACGGAGC GGATGTGACC ACCAAATCGA AGAAAGGTAC CTCCGCGTTG 
AACGTTATCA CCCGGAAGAC CCCGGCTTCA GTGGCCATGA TCAGACAGAA ACTGGACGCA 
GCTATTACGC TGCACCACTC GCAGGACCCC GTGAACCGTG AGGTGGAATT GGAGCTGGAC 
TTCCGTCAGC TGCTACAGCA CTGTCATCCG CGCGAGATCA GCTACCTAAA CACGTTCGTC 
GACGAGGGTC AGAAGGAGAT ACTGGAGCAC CCGCTCTGCT CCTCGTTTCT GTACATCAAG 
TGGGGCAAGA TCCGCAAGTA CTACATTGGC AGGCTCATCT TCTGCTTCAG CTTCGTGCTT 
TTCCTCACGC TTTACGTGCT GACTGCTTTG GCCCACAACT GCTACAATGG GAGCAAGAAC 
GACAACACGA CCATTCCAGC GCAGGAGTTG TGCCAGAAGC AATCCATCCT GGGAGATATG 
CTCAGAAACA ACCCCTTTGT GATGGAGATG CAATGGTGGG TTCTGGTTGC CATCACCATA 
GTGGAGATTT TTCGAAAGCT ATATGGCATT ACGGGCTACT CCTCATTTCG GCACTACGTA 
ACGCAAGTGG AGAACATAAT GGAGTGGTTC GTGATAACCA GCGTGTTTGT TATATCCTAC 
ATCTACACCA ACAAGACGTA CACGTTTCAA AACCACATAG GCGCCTTCGC CGTGCTGCTC 
GGTTGGACAA ATCTCATGTT GATGATAGGT CAGCTGCCGG TCTTCGATGT CTATGTGGCC 
ATGTACACGA GAGTCCAGGG GGAATTCGCA AAGCTATTTA TGGCCTATTC CTGCATGCTG 
ATTGGTTTTA CCATCAGTTT CTGTGTTATC TTCCCATCGT CGTCGTCGTT TGCCAATCCG 
TTTATGGGCT TCATAACAGT GCTGGTGATG ATGATTGGTG AACAGGATTT ATCGCTGCTC 
ATCAACGATC CCGAAGGCAA GGATCCACCT TTCCTGCTAG AAGTCAGCGC ACAAATCACT 
TTTGTTCTGT TCCTGCTGTT CGTGACCATC ATTCTGATGA ACCTGCTCGT GGGCATTGCA 
GTGCATGATA TCCAAGGATT GAAGAAGACG GCGGGGTTGT CTAAGCTCGT GCGTCAAACG 
AAGCTGATAA GCTACATAGA GTCGGCGCTA TTCAACGGCT ACCTTCCGAC ATGGCTGCGC 
AACTTGCTCC ATTACACCGC GCTGGTCTCT CCCCAAGCTT ATAGAGTGGT TCTGTGCGTC 
AAGCCACTGA ATCCCAGCGA GAAACGCTTG CCCCGGGAAA TCCTAATGAA GGCTTATGAA 
GTAGGAAAAA TGCGCAAGCA TTTCGGTCAT ACTATCTCGT CAAAGAACTC AGCCGAAAAC 
TATTTGTCGT ACAAGAACAA GTATAACAAC AATAATGGCG CCACGACCGG ATATGTACTA 
CCCGATTCGG ACCCGGATGC CGGGCAATTC ACCACACTAA CCACAAAGAT CGATGACAAT 
GCTGATCGGA TCGAGTTCCT CACCCAGGAG ATCCAGGAGC TAAAGCAGGC GCTCATCTCG 
CAGCAACAGC AAGCCAGCAA GGTGATCGAC AAGCTGCTGA TTGTGATCTC CAATCAGCAG 
AAACAGAATC TGCGCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wtrw ( NM_169200.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731193.1 Drosophila melanogaster water witch (wtrw), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa07964
Clone ID Related Accession (Same CDS sequence)	NM_169201.2 , NM_169200.2 , NM_206459.2
Accession Version	NM_169200.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	water witch, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169200.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAATC TTGGCTATAA GGAGGGCAGC ACCAAGCCCC GCCGCATGAC ACGTTCGATC 
AGCGTCGTTA CCAAGACCGA AGTGGAACAG CCGCTCACGG AGAATAATGC AAATAACCGC 
TTCAAATCGA TTCCACCCAA CTTGATGGTG CGATGGCGGA ACAACACTGA CGCCATGATC 
GAAGCCCAGT ACCCGACCGC TGGAGAATTC GAATACATGG AGTGTGGACC GTCGCCGCCG 
GCGGAGAGTG CGCCGAGCAT GTACGACAGC TTCGAGGAGC CGACCAGCGA GCTGAGCGTC 
CAGATATGCA ACGATACGCT GCGGATAAGC CTGATCGACC AGATGAAGAG CGCAGCGGGG 
CGCGTCAAGC TCTTCGAGGA CATCGAGCAG AGCAACGTGG TGGCCGAGGG CATACGGACG 
CACTTTGAGT CCGCCTCAAA GCTGGAGAAG AATCTCTGCT ACCTGTGGGC CGCGTACCTC 
AAGCGCTGGG ATCTGATCGA GAGTCTGCTG GAGGCGGGAG CCGACCTGCA CTTCTGCGAT 
CAGAACGGAA TATCAGCCTT GCACCTTAGC GCCTTCAGTG GCTGCTTGGC CACCTTGGGC 
CTTCTGGTCG CCAAGGGTTT GAATGTCAAT CTGCAGTCCA AGTGCTACAC CCCTCTGCAC 
TGCGCAGCCT TTGGAAATGC TGCGGAGGCA GCAAAGCTAC TGATCAACAA TGGGGCGGAC 
ATATCCAAGG ACACCAGCAA GCCAAACTGC GAGGAGAGCC TGCTGCACTG CGCCGTGCGT 
TCCAATGCCC TGGAGTGTCT GCAGATCTTC ATTGCCGAAG GCGCCGACGT CAACTCGCTC 
AAACCGAATG GCACCAATGC CATTCACCTG GCAGCTGATC TGGGCAATAT TCAGTGTCTG 
GAAGCCTTAC TGAATGCTCC CAATGCAGAT GCCAATGTGC GTATCTGCAT CCGCGAAAAG 
GAGTCCACAG CCTTGCATCT GGCAGCAGAT GAGGGCAACG TGGAGTGTGT GGACTTACTC 
CTGGCCAAAG GTGCTGATGC GAAGTTGAAG AACCATCGCG GTTTCACACC GCTCCACCTG 
GCAGCTAGAA CTTCCAGTCT GGACTGCGTA GAATCGTTGC TGAGGAATGG CAATGCCGAC 
GCCAATGCCG AGGACTTCGA TCACCGTACT CCGCTTCACG CGGCGGTGGG AAAATCGGAG 
AACGCCTATG ACATCATGGA AACGCTCATC CAGTGGGGTG CCAATGTCAA TCATAAGGAC 
ATTTACGGGT TTACAGCTCT TCACCTGGCT GCGTTGGATG GGCTGGTGCA GTGCGTCGAG 
ATGCTGATAT TCCACGGAGC GGATGTGACC ACCAAATCGA AGAAAGGTAC CTCCGCGTTG 
AACGTTATCA CCCGGAAGAC CCCGGCTTCA GTGGCCATGA TCAGACAGAA ACTGGACGCA 
GCTATTACGC TGCACCACTC GCAGGACCCC GTGAACCGTG AGGTGGAATT GGAGCTGGAC 
TTCCGTCAGC TGCTACAGCA CTGTCATCCG CGCGAGATCA GCTACCTAAA CACGTTCGTC 
GACGAGGGTC AGAAGGAGAT ACTGGAGCAC CCGCTCTGCT CCTCGTTTCT GTACATCAAG 
TGGGGCAAGA TCCGCAAGTA CTACATTGGC AGGCTCATCT TCTGCTTCAG CTTCGTGCTT 
TTCCTCACGC TTTACGTGCT GACTGCTTTG GCCCACAACT GCTACAATGG GAGCAAGAAC 
GACAACACGA CCATTCCAGC GCAGGAGTTG TGCCAGAAGC AATCCATCCT GGGAGATATG 
CTCAGAAACA ACCCCTTTGT GATGGAGATG CAATGGTGGG TTCTGGTTGC CATCACCATA 
GTGGAGATTT TTCGAAAGCT ATATGGCATT ACGGGCTACT CCTCATTTCG GCACTACGTA 
ACGCAAGTGG AGAACATAAT GGAGTGGTTC GTGATAACCA GCGTGTTTGT TATATCCTAC 
ATCTACACCA ACAAGACGTA CACGTTTCAA AACCACATAG GCGCCTTCGC CGTGCTGCTC 
GGTTGGACAA ATCTCATGTT GATGATAGGT CAGCTGCCGG TCTTCGATGT CTATGTGGCC 
ATGTACACGA GAGTCCAGGG GGAATTCGCA AAGCTATTTA TGGCCTATTC CTGCATGCTG 
ATTGGTTTTA CCATCAGTTT CTGTGTTATC TTCCCATCGT CGTCGTCGTT TGCCAATCCG 
TTTATGGGCT TCATAACAGT GCTGGTGATG ATGATTGGTG AACAGGATTT ATCGCTGCTC 
ATCAACGATC CCGAAGGCAA GGATCCACCT TTCCTGCTAG AAGTCAGCGC ACAAATCACT 
TTTGTTCTGT TCCTGCTGTT CGTGACCATC ATTCTGATGA ACCTGCTCGT GGGCATTGCA 
GTGCATGATA TCCAAGGATT GAAGAAGACG GCGGGGTTGT CTAAGCTCGT GCGTCAAACG 
AAGCTGATAA GCTACATAGA GTCGGCGCTA TTCAACGGCT ACCTTCCGAC ATGGCTGCGC 
AACTTGCTCC ATTACACCGC GCTGGTCTCT CCCCAAGCTT ATAGAGTGGT TCTGTGCGTC 
AAGCCACTGA ATCCCAGCGA GAAACGCTTG CCCCGGGAAA TCCTAATGAA GGCTTATGAA 
GTAGGAAAAA TGCGCAAGCA TTTCGGTCAT ACTATCTCGT CAAAGAACTC AGCCGAAAAC 
TATTTGTCGT ACAAGAACAA GTATAACAAC AATAATGGCG CCACGACCGG ATATGTACTA 
CCCGATTCGG ACCCGGATGC CGGGCAATTC ACCACACTAA CCACAAAGAT CGATGACAAT 
GCTGATCGGA TCGAGTTCCT CACCCAGGAG ATCCAGGAGC TAAAGCAGGC GCTCATCTCG 
CAGCAACAGC AAGCCAGCAA GGTGATCGAC AAGCTGCTGA TTGTGATCTC CAATCAGCAG 
AAACAGAATC TGCGCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731193.1
CDS	298..3258
Translation	MENLGYKEGSTKPRRMTRSISVVTKTEVEQPLTENNANNRFKSIPPNLMVRWRNNTDAMIEAQYPTAGEFEYMECGPSPPAESAPSMYDSFEEPTSELSVQICNDTLRISLIDQMKSAAGRVKLFEDIEQSNVVAEGIRTHFESASKLEKNLCYLWAAYLKRWDLIESLLEAGADLHFCDQNGISALHLSAFSGCLATLGLLVAKGLNVNLQSKCYTPLHCAAFGNAAEAAKLLINNGADISKDTSKPNCEESLLHCAVRSNALECLQIFIAEGADVNSLKPNGTNAIHLAADLGNIQCLEALLNAPNADANVRICIREKESTALHLAADEGNVECVDLLLAKGADAKLKNHRGFTPLHLAARTSSLDCVESLLRNGNADANAEDFDHRTPLHAAVGKSENAYDIMETLIQWGANVNHKDIYGFTALHLAALDGLVQCVEMLIFHGADVTTKSKKGTSALNVITRKTPASVAMIRQKLDAAITLHHSQDPVNREVELELDFRQLLQHCHPREISYLNTFVDEGQKEILEHPLCSSFLYIKWGKIRKYYIGRLIFCFSFVLFLTLYVLTALAHNCYNGSKNDNTTIPAQELCQKQSILGDMLRNNPFVMEMQWWVLVAITIVEIFRKLYGITGYSSFRHYVTQVENIMEWFVITSVFVISYIYTNKTYTFQNHIGAFAVLLGWTNLMLMIGQLPVFDVYVAMYTRVQGEFAKLFMAYSCMLIGFTISFCVIFPSSSSFANPFMGFITVLVMMIGEQDLSLLINDPEGKDPPFLLEVSAQITFVLFLLFVTIILMNLLVGIAVHDIQGLKKTAGLSKLVRQTKLISYIESALFNGYLPTWLRNLLHYTALVSPQAYRVVLCVKPLNPSEKRLPREILMKAYEVGKMRKHFGHTISSKNSAENYLSYKNKYNNNNGATTGYVLPDSDPDAGQFTTLTTKIDDNADRIEFLTQEIQELKQALISQQQQASKVIDKLLIVISNQQKQNLRK

Target ORF information:

RefSeq Version	NM_169200.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster water witch (wtrw), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169200.2

ORF Insert Sequence:

ATGGAGAATC TTGGCTATAA GGAGGGCAGC ACCAAGCCCC GCCGCATGAC ACGTTCGATC 
AGCGTCGTTA CCAAGACCGA AGTGGAACAG CCGCTCACGG AGAATAATGC AAATAACCGC 
TTCAAATCGA TTCCACCCAA CTTGATGGTG CGATGGCGGA ACAACACTGA CGCCATGATC 
GAAGCCCAGT ACCCGACCGC TGGAGAATTC GAATACATGG AGTGTGGACC GTCGCCGCCG 
GCGGAGAGTG CGCCGAGCAT GTACGACAGC TTCGAGGAGC CGACCAGCGA GCTGAGCGTC 
CAGATATGCA ACGATACGCT GCGGATAAGC CTGATCGACC AGATGAAGAG CGCAGCGGGG 
CGCGTCAAGC TCTTCGAGGA CATCGAGCAG AGCAACGTGG TGGCCGAGGG CATACGGACG 
CACTTTGAGT CCGCCTCAAA GCTGGAGAAG AATCTCTGCT ACCTGTGGGC CGCGTACCTC 
AAGCGCTGGG ATCTGATCGA GAGTCTGCTG GAGGCGGGAG CCGACCTGCA CTTCTGCGAT 
CAGAACGGAA TATCAGCCTT GCACCTTAGC GCCTTCAGTG GCTGCTTGGC CACCTTGGGC 
CTTCTGGTCG CCAAGGGTTT GAATGTCAAT CTGCAGTCCA AGTGCTACAC CCCTCTGCAC 
TGCGCAGCCT TTGGAAATGC TGCGGAGGCA GCAAAGCTAC TGATCAACAA TGGGGCGGAC 
ATATCCAAGG ACACCAGCAA GCCAAACTGC GAGGAGAGCC TGCTGCACTG CGCCGTGCGT 
TCCAATGCCC TGGAGTGTCT GCAGATCTTC ATTGCCGAAG GCGCCGACGT CAACTCGCTC 
AAACCGAATG GCACCAATGC CATTCACCTG GCAGCTGATC TGGGCAATAT TCAGTGTCTG 
GAAGCCTTAC TGAATGCTCC CAATGCAGAT GCCAATGTGC GTATCTGCAT CCGCGAAAAG 
GAGTCCACAG CCTTGCATCT GGCAGCAGAT GAGGGCAACG TGGAGTGTGT GGACTTACTC 
CTGGCCAAAG GTGCTGATGC GAAGTTGAAG AACCATCGCG GTTTCACACC GCTCCACCTG 
GCAGCTAGAA CTTCCAGTCT GGACTGCGTA GAATCGTTGC TGAGGAATGG CAATGCCGAC 
GCCAATGCCG AGGACTTCGA TCACCGTACT CCGCTTCACG CGGCGGTGGG AAAATCGGAG 
AACGCCTATG ACATCATGGA AACGCTCATC CAGTGGGGTG CCAATGTCAA TCATAAGGAC 
ATTTACGGGT TTACAGCTCT TCACCTGGCT GCGTTGGATG GGCTGGTGCA GTGCGTCGAG 
ATGCTGATAT TCCACGGAGC GGATGTGACC ACCAAATCGA AGAAAGGTAC CTCCGCGTTG 
AACGTTATCA CCCGGAAGAC CCCGGCTTCA GTGGCCATGA TCAGACAGAA ACTGGACGCA 
GCTATTACGC TGCACCACTC GCAGGACCCC GTGAACCGTG AGGTGGAATT GGAGCTGGAC 
TTCCGTCAGC TGCTACAGCA CTGTCATCCG CGCGAGATCA GCTACCTAAA CACGTTCGTC 
GACGAGGGTC AGAAGGAGAT ACTGGAGCAC CCGCTCTGCT CCTCGTTTCT GTACATCAAG 
TGGGGCAAGA TCCGCAAGTA CTACATTGGC AGGCTCATCT TCTGCTTCAG CTTCGTGCTT 
TTCCTCACGC TTTACGTGCT GACTGCTTTG GCCCACAACT GCTACAATGG GAGCAAGAAC 
GACAACACGA CCATTCCAGC GCAGGAGTTG TGCCAGAAGC AATCCATCCT GGGAGATATG 
CTCAGAAACA ACCCCTTTGT GATGGAGATG CAATGGTGGG TTCTGGTTGC CATCACCATA 
GTGGAGATTT TTCGAAAGCT ATATGGCATT ACGGGCTACT CCTCATTTCG GCACTACGTA 
ACGCAAGTGG AGAACATAAT GGAGTGGTTC GTGATAACCA GCGTGTTTGT TATATCCTAC 
ATCTACACCA ACAAGACGTA CACGTTTCAA AACCACATAG GCGCCTTCGC CGTGCTGCTC 
GGTTGGACAA ATCTCATGTT GATGATAGGT CAGCTGCCGG TCTTCGATGT CTATGTGGCC 
ATGTACACGA GAGTCCAGGG GGAATTCGCA AAGCTATTTA TGGCCTATTC CTGCATGCTG 
ATTGGTTTTA CCATCAGTTT CTGTGTTATC TTCCCATCGT CGTCGTCGTT TGCCAATCCG 
TTTATGGGCT TCATAACAGT GCTGGTGATG ATGATTGGTG AACAGGATTT ATCGCTGCTC 
ATCAACGATC CCGAAGGCAA GGATCCACCT TTCCTGCTAG AAGTCAGCGC ACAAATCACT 
TTTGTTCTGT TCCTGCTGTT CGTGACCATC ATTCTGATGA ACCTGCTCGT GGGCATTGCA 
GTGCATGATA TCCAAGGATT GAAGAAGACG GCGGGGTTGT CTAAGCTCGT GCGTCAAACG 
AAGCTGATAA GCTACATAGA GTCGGCGCTA TTCAACGGCT ACCTTCCGAC ATGGCTGCGC 
AACTTGCTCC ATTACACCGC GCTGGTCTCT CCCCAAGCTT ATAGAGTGGT TCTGTGCGTC 
AAGCCACTGA ATCCCAGCGA GAAACGCTTG CCCCGGGAAA TCCTAATGAA GGCTTATGAA 
GTAGGAAAAA TGCGCAAGCA TTTCGGTCAT ACTATCTCGT CAAAGAACTC AGCCGAAAAC 
TATTTGTCGT ACAAGAACAA GTATAACAAC AATAATGGCG CCACGACCGG ATATGTACTA 
CCCGATTCGG ACCCGGATGC CGGGCAATTC ACCACACTAA CCACAAAGAT CGATGACAAT 
GCTGATCGGA TCGAGTTCCT CACCCAGGAG ATCCAGGAGC TAAAGCAGGC GCTCATCTCG 
CAGCAACAGC AAGCCAGCAA GGTGATCGAC AAGCTGCTGA TTGTGATCTC CAATCAGCAG 
AAACAGAATC TGCGCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wtrw ( NM_206459.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996181.1 Drosophila melanogaster water witch (wtrw), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa07964
Clone ID Related Accession (Same CDS sequence)	NM_169201.2 , NM_169200.2 , NM_206459.2
Accession Version	NM_206459.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	water witch, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_206459.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAATC TTGGCTATAA GGAGGGCAGC ACCAAGCCCC GCCGCATGAC ACGTTCGATC 
AGCGTCGTTA CCAAGACCGA AGTGGAACAG CCGCTCACGG AGAATAATGC AAATAACCGC 
TTCAAATCGA TTCCACCCAA CTTGATGGTG CGATGGCGGA ACAACACTGA CGCCATGATC 
GAAGCCCAGT ACCCGACCGC TGGAGAATTC GAATACATGG AGTGTGGACC GTCGCCGCCG 
GCGGAGAGTG CGCCGAGCAT GTACGACAGC TTCGAGGAGC CGACCAGCGA GCTGAGCGTC 
CAGATATGCA ACGATACGCT GCGGATAAGC CTGATCGACC AGATGAAGAG CGCAGCGGGG 
CGCGTCAAGC TCTTCGAGGA CATCGAGCAG AGCAACGTGG TGGCCGAGGG CATACGGACG 
CACTTTGAGT CCGCCTCAAA GCTGGAGAAG AATCTCTGCT ACCTGTGGGC CGCGTACCTC 
AAGCGCTGGG ATCTGATCGA GAGTCTGCTG GAGGCGGGAG CCGACCTGCA CTTCTGCGAT 
CAGAACGGAA TATCAGCCTT GCACCTTAGC GCCTTCAGTG GCTGCTTGGC CACCTTGGGC 
CTTCTGGTCG CCAAGGGTTT GAATGTCAAT CTGCAGTCCA AGTGCTACAC CCCTCTGCAC 
TGCGCAGCCT TTGGAAATGC TGCGGAGGCA GCAAAGCTAC TGATCAACAA TGGGGCGGAC 
ATATCCAAGG ACACCAGCAA GCCAAACTGC GAGGAGAGCC TGCTGCACTG CGCCGTGCGT 
TCCAATGCCC TGGAGTGTCT GCAGATCTTC ATTGCCGAAG GCGCCGACGT CAACTCGCTC 
AAACCGAATG GCACCAATGC CATTCACCTG GCAGCTGATC TGGGCAATAT TCAGTGTCTG 
GAAGCCTTAC TGAATGCTCC CAATGCAGAT GCCAATGTGC GTATCTGCAT CCGCGAAAAG 
GAGTCCACAG CCTTGCATCT GGCAGCAGAT GAGGGCAACG TGGAGTGTGT GGACTTACTC 
CTGGCCAAAG GTGCTGATGC GAAGTTGAAG AACCATCGCG GTTTCACACC GCTCCACCTG 
GCAGCTAGAA CTTCCAGTCT GGACTGCGTA GAATCGTTGC TGAGGAATGG CAATGCCGAC 
GCCAATGCCG AGGACTTCGA TCACCGTACT CCGCTTCACG CGGCGGTGGG AAAATCGGAG 
AACGCCTATG ACATCATGGA AACGCTCATC CAGTGGGGTG CCAATGTCAA TCATAAGGAC 
ATTTACGGGT TTACAGCTCT TCACCTGGCT GCGTTGGATG GGCTGGTGCA GTGCGTCGAG 
ATGCTGATAT TCCACGGAGC GGATGTGACC ACCAAATCGA AGAAAGGTAC CTCCGCGTTG 
AACGTTATCA CCCGGAAGAC CCCGGCTTCA GTGGCCATGA TCAGACAGAA ACTGGACGCA 
GCTATTACGC TGCACCACTC GCAGGACCCC GTGAACCGTG AGGTGGAATT GGAGCTGGAC 
TTCCGTCAGC TGCTACAGCA CTGTCATCCG CGCGAGATCA GCTACCTAAA CACGTTCGTC 
GACGAGGGTC AGAAGGAGAT ACTGGAGCAC CCGCTCTGCT CCTCGTTTCT GTACATCAAG 
TGGGGCAAGA TCCGCAAGTA CTACATTGGC AGGCTCATCT TCTGCTTCAG CTTCGTGCTT 
TTCCTCACGC TTTACGTGCT GACTGCTTTG GCCCACAACT GCTACAATGG GAGCAAGAAC 
GACAACACGA CCATTCCAGC GCAGGAGTTG TGCCAGAAGC AATCCATCCT GGGAGATATG 
CTCAGAAACA ACCCCTTTGT GATGGAGATG CAATGGTGGG TTCTGGTTGC CATCACCATA 
GTGGAGATTT TTCGAAAGCT ATATGGCATT ACGGGCTACT CCTCATTTCG GCACTACGTA 
ACGCAAGTGG AGAACATAAT GGAGTGGTTC GTGATAACCA GCGTGTTTGT TATATCCTAC 
ATCTACACCA ACAAGACGTA CACGTTTCAA AACCACATAG GCGCCTTCGC CGTGCTGCTC 
GGTTGGACAA ATCTCATGTT GATGATAGGT CAGCTGCCGG TCTTCGATGT CTATGTGGCC 
ATGTACACGA GAGTCCAGGG GGAATTCGCA AAGCTATTTA TGGCCTATTC CTGCATGCTG 
ATTGGTTTTA CCATCAGTTT CTGTGTTATC TTCCCATCGT CGTCGTCGTT TGCCAATCCG 
TTTATGGGCT TCATAACAGT GCTGGTGATG ATGATTGGTG AACAGGATTT ATCGCTGCTC 
ATCAACGATC CCGAAGGCAA GGATCCACCT TTCCTGCTAG AAGTCAGCGC ACAAATCACT 
TTTGTTCTGT TCCTGCTGTT CGTGACCATC ATTCTGATGA ACCTGCTCGT GGGCATTGCA 
GTGCATGATA TCCAAGGATT GAAGAAGACG GCGGGGTTGT CTAAGCTCGT GCGTCAAACG 
AAGCTGATAA GCTACATAGA GTCGGCGCTA TTCAACGGCT ACCTTCCGAC ATGGCTGCGC 
AACTTGCTCC ATTACACCGC GCTGGTCTCT CCCCAAGCTT ATAGAGTGGT TCTGTGCGTC 
AAGCCACTGA ATCCCAGCGA GAAACGCTTG CCCCGGGAAA TCCTAATGAA GGCTTATGAA 
GTAGGAAAAA TGCGCAAGCA TTTCGGTCAT ACTATCTCGT CAAAGAACTC AGCCGAAAAC 
TATTTGTCGT ACAAGAACAA GTATAACAAC AATAATGGCG CCACGACCGG ATATGTACTA 
CCCGATTCGG ACCCGGATGC CGGGCAATTC ACCACACTAA CCACAAAGAT CGATGACAAT 
GCTGATCGGA TCGAGTTCCT CACCCAGGAG ATCCAGGAGC TAAAGCAGGC GCTCATCTCG 
CAGCAACAGC AAGCCAGCAA GGTGATCGAC AAGCTGCTGA TTGTGATCTC CAATCAGCAG 
AAACAGAATC TGCGCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996181.1
CDS	405..3365
Translation	MENLGYKEGSTKPRRMTRSISVVTKTEVEQPLTENNANNRFKSIPPNLMVRWRNNTDAMIEAQYPTAGEFEYMECGPSPPAESAPSMYDSFEEPTSELSVQICNDTLRISLIDQMKSAAGRVKLFEDIEQSNVVAEGIRTHFESASKLEKNLCYLWAAYLKRWDLIESLLEAGADLHFCDQNGISALHLSAFSGCLATLGLLVAKGLNVNLQSKCYTPLHCAAFGNAAEAAKLLINNGADISKDTSKPNCEESLLHCAVRSNALECLQIFIAEGADVNSLKPNGTNAIHLAADLGNIQCLEALLNAPNADANVRICIREKESTALHLAADEGNVECVDLLLAKGADAKLKNHRGFTPLHLAARTSSLDCVESLLRNGNADANAEDFDHRTPLHAAVGKSENAYDIMETLIQWGANVNHKDIYGFTALHLAALDGLVQCVEMLIFHGADVTTKSKKGTSALNVITRKTPASVAMIRQKLDAAITLHHSQDPVNREVELELDFRQLLQHCHPREISYLNTFVDEGQKEILEHPLCSSFLYIKWGKIRKYYIGRLIFCFSFVLFLTLYVLTALAHNCYNGSKNDNTTIPAQELCQKQSILGDMLRNNPFVMEMQWWVLVAITIVEIFRKLYGITGYSSFRHYVTQVENIMEWFVITSVFVISYIYTNKTYTFQNHIGAFAVLLGWTNLMLMIGQLPVFDVYVAMYTRVQGEFAKLFMAYSCMLIGFTISFCVIFPSSSSFANPFMGFITVLVMMIGEQDLSLLINDPEGKDPPFLLEVSAQITFVLFLLFVTIILMNLLVGIAVHDIQGLKKTAGLSKLVRQTKLISYIESALFNGYLPTWLRNLLHYTALVSPQAYRVVLCVKPLNPSEKRLPREILMKAYEVGKMRKHFGHTISSKNSAENYLSYKNKYNNNNGATTGYVLPDSDPDAGQFTTLTTKIDDNADRIEFLTQEIQELKQALISQQQQASKVIDKLLIVISNQQKQNLRK

Target ORF information:

RefSeq Version	NM_206459.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster water witch (wtrw), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206459.2

ORF Insert Sequence:

ATGGAGAATC TTGGCTATAA GGAGGGCAGC ACCAAGCCCC GCCGCATGAC ACGTTCGATC 
AGCGTCGTTA CCAAGACCGA AGTGGAACAG CCGCTCACGG AGAATAATGC AAATAACCGC 
TTCAAATCGA TTCCACCCAA CTTGATGGTG CGATGGCGGA ACAACACTGA CGCCATGATC 
GAAGCCCAGT ACCCGACCGC TGGAGAATTC GAATACATGG AGTGTGGACC GTCGCCGCCG 
GCGGAGAGTG CGCCGAGCAT GTACGACAGC TTCGAGGAGC CGACCAGCGA GCTGAGCGTC 
CAGATATGCA ACGATACGCT GCGGATAAGC CTGATCGACC AGATGAAGAG CGCAGCGGGG 
CGCGTCAAGC TCTTCGAGGA CATCGAGCAG AGCAACGTGG TGGCCGAGGG CATACGGACG 
CACTTTGAGT CCGCCTCAAA GCTGGAGAAG AATCTCTGCT ACCTGTGGGC CGCGTACCTC 
AAGCGCTGGG ATCTGATCGA GAGTCTGCTG GAGGCGGGAG CCGACCTGCA CTTCTGCGAT 
CAGAACGGAA TATCAGCCTT GCACCTTAGC GCCTTCAGTG GCTGCTTGGC CACCTTGGGC 
CTTCTGGTCG CCAAGGGTTT GAATGTCAAT CTGCAGTCCA AGTGCTACAC CCCTCTGCAC 
TGCGCAGCCT TTGGAAATGC TGCGGAGGCA GCAAAGCTAC TGATCAACAA TGGGGCGGAC 
ATATCCAAGG ACACCAGCAA GCCAAACTGC GAGGAGAGCC TGCTGCACTG CGCCGTGCGT 
TCCAATGCCC TGGAGTGTCT GCAGATCTTC ATTGCCGAAG GCGCCGACGT CAACTCGCTC 
AAACCGAATG GCACCAATGC CATTCACCTG GCAGCTGATC TGGGCAATAT TCAGTGTCTG 
GAAGCCTTAC TGAATGCTCC CAATGCAGAT GCCAATGTGC GTATCTGCAT CCGCGAAAAG 
GAGTCCACAG CCTTGCATCT GGCAGCAGAT GAGGGCAACG TGGAGTGTGT GGACTTACTC 
CTGGCCAAAG GTGCTGATGC GAAGTTGAAG AACCATCGCG GTTTCACACC GCTCCACCTG 
GCAGCTAGAA CTTCCAGTCT GGACTGCGTA GAATCGTTGC TGAGGAATGG CAATGCCGAC 
GCCAATGCCG AGGACTTCGA TCACCGTACT CCGCTTCACG CGGCGGTGGG AAAATCGGAG 
AACGCCTATG ACATCATGGA AACGCTCATC CAGTGGGGTG CCAATGTCAA TCATAAGGAC 
ATTTACGGGT TTACAGCTCT TCACCTGGCT GCGTTGGATG GGCTGGTGCA GTGCGTCGAG 
ATGCTGATAT TCCACGGAGC GGATGTGACC ACCAAATCGA AGAAAGGTAC CTCCGCGTTG 
AACGTTATCA CCCGGAAGAC CCCGGCTTCA GTGGCCATGA TCAGACAGAA ACTGGACGCA 
GCTATTACGC TGCACCACTC GCAGGACCCC GTGAACCGTG AGGTGGAATT GGAGCTGGAC 
TTCCGTCAGC TGCTACAGCA CTGTCATCCG CGCGAGATCA GCTACCTAAA CACGTTCGTC 
GACGAGGGTC AGAAGGAGAT ACTGGAGCAC CCGCTCTGCT CCTCGTTTCT GTACATCAAG 
TGGGGCAAGA TCCGCAAGTA CTACATTGGC AGGCTCATCT TCTGCTTCAG CTTCGTGCTT 
TTCCTCACGC TTTACGTGCT GACTGCTTTG GCCCACAACT GCTACAATGG GAGCAAGAAC 
GACAACACGA CCATTCCAGC GCAGGAGTTG TGCCAGAAGC AATCCATCCT GGGAGATATG 
CTCAGAAACA ACCCCTTTGT GATGGAGATG CAATGGTGGG TTCTGGTTGC CATCACCATA 
GTGGAGATTT TTCGAAAGCT ATATGGCATT ACGGGCTACT CCTCATTTCG GCACTACGTA 
ACGCAAGTGG AGAACATAAT GGAGTGGTTC GTGATAACCA GCGTGTTTGT TATATCCTAC 
ATCTACACCA ACAAGACGTA CACGTTTCAA AACCACATAG GCGCCTTCGC CGTGCTGCTC 
GGTTGGACAA ATCTCATGTT GATGATAGGT CAGCTGCCGG TCTTCGATGT CTATGTGGCC 
ATGTACACGA GAGTCCAGGG GGAATTCGCA AAGCTATTTA TGGCCTATTC CTGCATGCTG 
ATTGGTTTTA CCATCAGTTT CTGTGTTATC TTCCCATCGT CGTCGTCGTT TGCCAATCCG 
TTTATGGGCT TCATAACAGT GCTGGTGATG ATGATTGGTG AACAGGATTT ATCGCTGCTC 
ATCAACGATC CCGAAGGCAA GGATCCACCT TTCCTGCTAG AAGTCAGCGC ACAAATCACT 
TTTGTTCTGT TCCTGCTGTT CGTGACCATC ATTCTGATGA ACCTGCTCGT GGGCATTGCA 
GTGCATGATA TCCAAGGATT GAAGAAGACG GCGGGGTTGT CTAAGCTCGT GCGTCAAACG 
AAGCTGATAA GCTACATAGA GTCGGCGCTA TTCAACGGCT ACCTTCCGAC ATGGCTGCGC 
AACTTGCTCC ATTACACCGC GCTGGTCTCT CCCCAAGCTT ATAGAGTGGT TCTGTGCGTC 
AAGCCACTGA ATCCCAGCGA GAAACGCTTG CCCCGGGAAA TCCTAATGAA GGCTTATGAA 
GTAGGAAAAA TGCGCAAGCA TTTCGGTCAT ACTATCTCGT CAAAGAACTC AGCCGAAAAC 
TATTTGTCGT ACAAGAACAA GTATAACAAC AATAATGGCG CCACGACCGG ATATGTACTA 
CCCGATTCGG ACCCGGATGC CGGGCAATTC ACCACACTAA CCACAAAGAT CGATGACAAT 
GCTGATCGGA TCGAGTTCCT CACCCAGGAG ATCCAGGAGC TAAAGCAGGC GCTCATCTCG 
CAGCAACAGC AAGCCAGCAA GGTGATCGAC AAGCTGCTGA TTGTGATCTC CAATCAGCAG 
AAACAGAATC TGCGCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wtrw ( NM_001300285.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287214.1 Drosophila melanogaster water witch (wtrw), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa22060
Clone ID Related Accession (Same CDS sequence)	NM_001300285.1
Accession Version	NM_001300285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	water witch, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAATTAG AAATTGAAAA CAGCTCACTG TCGAATTGGC ATCATCCGTG GATATGGCTG 
CAAACGGTCT TCAAATCGAT TCCACCCAAC TTGATGGTGC GATGGCGGAA CAACACTGAC 
GCCATGATCG AAGCCCAGTA CCCGACCGCT GGAGAATTCG AATACATGGA GTGTGGACCG 
TCGCCGCCGG CGGAGAGTGC GCCGAGCATG TACGACAGCT TCGAGGAGCC GACCAGCGAG 
CTGAGCGTCC AGATATGCAA CGATACGCTG CGGATAAGCC TGATCGACCA GATGAAGAGC 
GCAGCGGGGC GCGTCAAGCT CTTCGAGGAC ATCGAGCAGA GCAACGTGGT GGCCGAGGGC 
ATACGGACGC ACTTTGAGTC CGCCTCAAAG CTGGAGAAGA ATCTCTGCTA CCTGTGGGCC 
GCGTACCTCA AGCGCTGGGA TCTGATCGAG AGTCTGCTGG AGGCGGGAGC CGACCTGCAC 
TTCTGCGATC AGAACGGAAT ATCAGCCTTG CACCTTAGCG CCTTCAGTGG CTGCTTGGCC 
ACCTTGGGCC TTCTGGTCGC CAAGGGTTTG AATGTCAATC TGCAGTCCAA GTGCTACACC 
CCTCTGCACT GCGCAGCCTT TGGAAATGCT GCGGAGGCAG CAAAGCTACT GATCAACAAT 
GGGGCGGACA TATCCAAGGA CACCAGCAAG CCAAACTGCG AGGAGAGCCT GCTGCACTGC 
GCCGTGCGTT CCAATGCCCT GGAGTGTCTG CAGATCTTCA TTGCCGAAGG CGCCGACGTC 
AACTCGCTCA AACCGAATGG CACCAATGCC ATTCACCTGG CAGCTGATCT GGGCAATATT 
CAGTGTCTGG AAGCCTTACT GAATGCTCCC AATGCAGATG CCAATGTGCG TATCTGCATC 
CGCGAAAAGG AGTCCACAGC CTTGCATCTG GCAGCAGATG AGGGCAACGT GGAGTGTGTG 
GACTTACTCC TGGCCAAAGG TGCTGATGCG AAGTTGAAGA ACCATCGCGG TTTCACACCG 
CTCCACCTGG CAGCTAGAAC TTCCAGTCTG GACTGCGTAG AATCGTTGCT GAGGAATGGC 
AATGCCGACG CCAATGCCGA GGACTTCGAT CACCGTACTC CGCTTCACGC GGCGGTGGGA 
AAATCGGAGA ACGCCTATGA CATCATGGAA ACGCTCATCC AGTGGGGTGC CAATGTCAAT 
CATAAGGACA TTTACGGGTT TACAGCTCTT CACCTGGCTG CGTTGGATGG GCTGGTGCAG 
TGCGTCGAGA TGCTGATATT CCACGGAGCG GATGTGACCA CCAAATCGAA GAAAGGTACC 
TCCGCGTTGA ACGTTATCAC CCGGAAGACC CCGGCTTCAG TGGCCATGAT CAGACAGAAA 
CTGGACGCAG CTATTACGCT GCACCACTCG CAGGACCCCG TGAACCGTGA GGTGGAATTG 
GAGCTGGACT TCCGTCAGCT GCTACAGCAC TGTCATCCGC GCGAGATCAG CTACCTAAAC 
ACGTTCGTCG ACGAGGGTCA GAAGGAGATA CTGGAGCACC CGCTCTGCTC CTCGTTTCTG 
TACATCAAGT GGGGCAAGAT CCGCAAGTAC TACATTGGCA GGCTCATCTT CTGCTTCAGC 
TTCGTGCTTT TCCTCACGCT TTACGTGCTG ACTGCTTTGG CCCACAACTG CTACAATGGG 
AGCAAGAACG ACAACACGAC CATTCCAGCG CAGGAGTTGT GCCAGAAGCA ATCCATCCTG 
GGAGATATGC TCAGAAACAA CCCCTTTGTG ATGGAGATGC AATGGTGGGT TCTGGTTGCC 
ATCACCATAG TGGAGATTTT TCGAAAGCTA TATGGCATTA CGGGCTACTC CTCATTTCGG 
CACTACGTAA CGCAAGTGGA GAACATAATG GAGTGGTTCG TGATAACCAG CGTGTTTGTT 
ATATCCTACA TCTACACCAA CAAGACGTAC ACGTTTCAAA ACCACATAGG CGCCTTCGCC 
GTGCTGCTCG GTTGGACAAA TCTCATGTTG ATGATAGGTC AGCTGCCGGT CTTCGATGTC 
TATGTGGCCA TGTACACGAG AGTCCAGGGG GAATTCGCAA AGCTATTTAT GGCCTATTCC 
TGCATGCTGA TTGGTTTTAC CATCAGTTTC TGTGTTATCT TCCCATCGTC GTCGTCGTTT 
GCCAATCCGT TTATGGGCTT CATAACAGTG CTGGTGATGA TGATTGGTGA ACAGGATTTA 
TCGCTGCTCA TCAACGATCC CGAAGGCAAG GATCCACCTT TCCTGCTAGA AGTCAGCGCA 
CAAATCACTT TTGTTCTGTT CCTGCTGTTC GTGACCATCA TTCTGATGAA CCTGCTCGTG 
GGCATTGCAG TGCATGATAT CCAAGGATTG AAGAAGACGG CGGGGTTGTC TAAGCTCGTG 
CGTCAAACGA AGCTGATAAG CTACATAGAG TCGGCGCTAT TCAACGGCTA CCTTCCGACA 
TGGCTGCGCA ACTTGCTCCA TTACACCGCG CTGGTCTCTC CCCAAGCTTA TAGAGTGGTT 
CTGTGCGTCA AGCCACTGAA TCCCAGCGAG AAACGCTTGC CCCGGGAAAT CCTAATGAAG 
GCTTATGAAG TAGGAAAAAT GCGCAAGCAT TTCGGTCATA CTATCTCGTC AAAGAACTCA 
GCCGAAAACT ATTTGTCGTA CAAGAACAAG TATAACAACA ATAATGGCGC CACGACCGGA 
TATGTACTAC CCGATTCGGA CCCGGATGCC GGGCAATTCA CCACACTAAC CACAAAGATC 
GATGACAATG CTGATCGGAT CGAGTTCCTC ACCCAGGAGA TCCAGGAGCT AAAGCAGGCG 
CTCATCTCGC AGCAACAGCA AGCCAGCAAG GTGATCGACA AGCTGCTGAT TGTGATCTCC 
AATCAGCAGA AACAGAATCT GCGCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287214.1
CDS	455..3364
Translation	MKLEIENSSLSNWHHPWIWLQTVFKSIPPNLMVRWRNNTDAMIEAQYPTAGEFEYMECGPSPPAESAPSMYDSFEEPTSELSVQICNDTLRISLIDQMKSAAGRVKLFEDIEQSNVVAEGIRTHFESASKLEKNLCYLWAAYLKRWDLIESLLEAGADLHFCDQNGISALHLSAFSGCLATLGLLVAKGLNVNLQSKCYTPLHCAAFGNAAEAAKLLINNGADISKDTSKPNCEESLLHCAVRSNALECLQIFIAEGADVNSLKPNGTNAIHLAADLGNIQCLEALLNAPNADANVRICIREKESTALHLAADEGNVECVDLLLAKGADAKLKNHRGFTPLHLAARTSSLDCVESLLRNGNADANAEDFDHRTPLHAAVGKSENAYDIMETLIQWGANVNHKDIYGFTALHLAALDGLVQCVEMLIFHGADVTTKSKKGTSALNVITRKTPASVAMIRQKLDAAITLHHSQDPVNREVELELDFRQLLQHCHPREISYLNTFVDEGQKEILEHPLCSSFLYIKWGKIRKYYIGRLIFCFSFVLFLTLYVLTALAHNCYNGSKNDNTTIPAQELCQKQSILGDMLRNNPFVMEMQWWVLVAITIVEIFRKLYGITGYSSFRHYVTQVENIMEWFVITSVFVISYIYTNKTYTFQNHIGAFAVLLGWTNLMLMIGQLPVFDVYVAMYTRVQGEFAKLFMAYSCMLIGFTISFCVIFPSSSSFANPFMGFITVLVMMIGEQDLSLLINDPEGKDPPFLLEVSAQITFVLFLLFVTIILMNLLVGIAVHDIQGLKKTAGLSKLVRQTKLISYIESALFNGYLPTWLRNLLHYTALVSPQAYRVVLCVKPLNPSEKRLPREILMKAYEVGKMRKHFGHTISSKNSAENYLSYKNKYNNNNGATTGYVLPDSDPDAGQFTTLTTKIDDNADRIEFLTQEIQELKQALISQQQQASKVIDKLLIVISNQQKQNLRK

Target ORF information:

RefSeq Version	NM_001300285.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster water witch (wtrw), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300285.1

ORF Insert Sequence:

ATGAAATTAG AAATTGAAAA CAGCTCACTG TCGAATTGGC ATCATCCGTG GATATGGCTG 
CAAACGGTCT TCAAATCGAT TCCACCCAAC TTGATGGTGC GATGGCGGAA CAACACTGAC 
GCCATGATCG AAGCCCAGTA CCCGACCGCT GGAGAATTCG AATACATGGA GTGTGGACCG 
TCGCCGCCGG CGGAGAGTGC GCCGAGCATG TACGACAGCT TCGAGGAGCC GACCAGCGAG 
CTGAGCGTCC AGATATGCAA CGATACGCTG CGGATAAGCC TGATCGACCA GATGAAGAGC 
GCAGCGGGGC GCGTCAAGCT CTTCGAGGAC ATCGAGCAGA GCAACGTGGT GGCCGAGGGC 
ATACGGACGC ACTTTGAGTC CGCCTCAAAG CTGGAGAAGA ATCTCTGCTA CCTGTGGGCC 
GCGTACCTCA AGCGCTGGGA TCTGATCGAG AGTCTGCTGG AGGCGGGAGC CGACCTGCAC 
TTCTGCGATC AGAACGGAAT ATCAGCCTTG CACCTTAGCG CCTTCAGTGG CTGCTTGGCC 
ACCTTGGGCC TTCTGGTCGC CAAGGGTTTG AATGTCAATC TGCAGTCCAA GTGCTACACC 
CCTCTGCACT GCGCAGCCTT TGGAAATGCT GCGGAGGCAG CAAAGCTACT GATCAACAAT 
GGGGCGGACA TATCCAAGGA CACCAGCAAG CCAAACTGCG AGGAGAGCCT GCTGCACTGC 
GCCGTGCGTT CCAATGCCCT GGAGTGTCTG CAGATCTTCA TTGCCGAAGG CGCCGACGTC 
AACTCGCTCA AACCGAATGG CACCAATGCC ATTCACCTGG CAGCTGATCT GGGCAATATT 
CAGTGTCTGG AAGCCTTACT GAATGCTCCC AATGCAGATG CCAATGTGCG TATCTGCATC 
CGCGAAAAGG AGTCCACAGC CTTGCATCTG GCAGCAGATG AGGGCAACGT GGAGTGTGTG 
GACTTACTCC TGGCCAAAGG TGCTGATGCG AAGTTGAAGA ACCATCGCGG TTTCACACCG 
CTCCACCTGG CAGCTAGAAC TTCCAGTCTG GACTGCGTAG AATCGTTGCT GAGGAATGGC 
AATGCCGACG CCAATGCCGA GGACTTCGAT CACCGTACTC CGCTTCACGC GGCGGTGGGA 
AAATCGGAGA ACGCCTATGA CATCATGGAA ACGCTCATCC AGTGGGGTGC CAATGTCAAT 
CATAAGGACA TTTACGGGTT TACAGCTCTT CACCTGGCTG CGTTGGATGG GCTGGTGCAG 
TGCGTCGAGA TGCTGATATT CCACGGAGCG GATGTGACCA CCAAATCGAA GAAAGGTACC 
TCCGCGTTGA ACGTTATCAC CCGGAAGACC CCGGCTTCAG TGGCCATGAT CAGACAGAAA 
CTGGACGCAG CTATTACGCT GCACCACTCG CAGGACCCCG TGAACCGTGA GGTGGAATTG 
GAGCTGGACT TCCGTCAGCT GCTACAGCAC TGTCATCCGC GCGAGATCAG CTACCTAAAC 
ACGTTCGTCG ACGAGGGTCA GAAGGAGATA CTGGAGCACC CGCTCTGCTC CTCGTTTCTG 
TACATCAAGT GGGGCAAGAT CCGCAAGTAC TACATTGGCA GGCTCATCTT CTGCTTCAGC 
TTCGTGCTTT TCCTCACGCT TTACGTGCTG ACTGCTTTGG CCCACAACTG CTACAATGGG 
AGCAAGAACG ACAACACGAC CATTCCAGCG CAGGAGTTGT GCCAGAAGCA ATCCATCCTG 
GGAGATATGC TCAGAAACAA CCCCTTTGTG ATGGAGATGC AATGGTGGGT TCTGGTTGCC 
ATCACCATAG TGGAGATTTT TCGAAAGCTA TATGGCATTA CGGGCTACTC CTCATTTCGG 
CACTACGTAA CGCAAGTGGA GAACATAATG GAGTGGTTCG TGATAACCAG CGTGTTTGTT 
ATATCCTACA TCTACACCAA CAAGACGTAC ACGTTTCAAA ACCACATAGG CGCCTTCGCC 
GTGCTGCTCG GTTGGACAAA TCTCATGTTG ATGATAGGTC AGCTGCCGGT CTTCGATGTC 
TATGTGGCCA TGTACACGAG AGTCCAGGGG GAATTCGCAA AGCTATTTAT GGCCTATTCC 
TGCATGCTGA TTGGTTTTAC CATCAGTTTC TGTGTTATCT TCCCATCGTC GTCGTCGTTT 
GCCAATCCGT TTATGGGCTT CATAACAGTG CTGGTGATGA TGATTGGTGA ACAGGATTTA 
TCGCTGCTCA TCAACGATCC CGAAGGCAAG GATCCACCTT TCCTGCTAGA AGTCAGCGCA 
CAAATCACTT TTGTTCTGTT CCTGCTGTTC GTGACCATCA TTCTGATGAA CCTGCTCGTG 
GGCATTGCAG TGCATGATAT CCAAGGATTG AAGAAGACGG CGGGGTTGTC TAAGCTCGTG 
CGTCAAACGA AGCTGATAAG CTACATAGAG TCGGCGCTAT TCAACGGCTA CCTTCCGACA 
TGGCTGCGCA ACTTGCTCCA TTACACCGCG CTGGTCTCTC CCCAAGCTTA TAGAGTGGTT 
CTGTGCGTCA AGCCACTGAA TCCCAGCGAG AAACGCTTGC CCCGGGAAAT CCTAATGAAG 
GCTTATGAAG TAGGAAAAAT GCGCAAGCAT TTCGGTCATA CTATCTCGTC AAAGAACTCA 
GCCGAAAACT ATTTGTCGTA CAAGAACAAG TATAACAACA ATAATGGCGC CACGACCGGA 
TATGTACTAC CCGATTCGGA CCCGGATGCC GGGCAATTCA CCACACTAAC CACAAAGATC 
GATGACAATG CTGATCGGAT CGAGTTCCTC ACCCAGGAGA TCCAGGAGCT AAAGCAGGCG 
CTCATCTCGC AGCAACAGCA AGCCAGCAAG GTGATCGACA AGCTGCTGAT TGTGATCTCC 
AATCAGCAGA AACAGAATCT GCGCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wtrw cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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