Dfd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dfd
Entrez Gene ID	40832
Full Name	Deformed
Synonyms	BG:DS00276.5,CG2189,DFD,DmDfd,Dmel\CG2189,EbR11,dfd,l(3)84Ae
General protein information	Preferred Names Deformed Names CG2189 gene product from transcript CG2189-RA CG2189-PA Dfd-PA deformed
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84A5-84A5\|3-47.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057853.3	NP_477201.1	deformed

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In conjunction with its receptor Neurotactin (Nrt) and the effector kinase Abelson tyrosine kinase (Abl), Ama is necessary in parallel to the cell-autonomous Dfd pathway for the correct specification of the maxillary identity of NB6-4
Title: Cell-Autonomous and Non-cell-autonomous Function of Hox Genes Specify Segmental Neuroblast Identity in the Gnathal Region of the Embryonic CNS in Drosophila.

Dfd controls expression of functional components of synapses, and is critical for the establishment and maintenance of the neuromuscular unit required for feeding behavior.
Title: Hox Function Is Required for the Development and Maintenance of the Drosophila Feeding Motor Unit.

Hox coordination of tissue morphogenesis relies on boundary fold formation through the modulation of DE-cad positioning
Title: The Hox gene Dfd controls organogenesis by shaping territorial border through regulation of basal DE-Cadherin distribution.

Dfd and Ultrabithorax (Ubx), another Hox TF specifying different morphological traits, interact with non-overlapping regions in vivo, despite their similar DNA binding preferences.
Title: The cis-regulatory code of Hox function in Drosophila.

Study shows that subtle differences in the way Dfd and Scr recognize their specific binding sites, in conjunction with non-DNA-binding domains, influence whether the target gene is transcriptionally activated or repressed.
Title: Dissecting the functional specificities of two Hox proteins.

The following Dfd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dfd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04129	NM_057853.3 Latest version!	Drosophila melanogaster deformed (Dfd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dfd ( NM_057853.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477201.1 Drosophila melanogaster deformed (Dfd), mRNA.

Price & Availability↑

CloneID	OFa04129
Clone ID Related Accession (Same CDS sequence)	NM_057853.3
Accession Version	NM_057853.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deformed
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057853.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTCTT TTCTCATGGG TTATCCGCAT GCACCACATC ATGTCCAGAG TCCCATGTCC 
ATGGGCAATG GCTTGGACCC AAAGTTTCCG CCGTTGGCCG ATGACTATCA TCACTACAAT 
GGACACTATT CGATGACCGC ATCTACGGGT CATATGAGTG GTGCCGTCGG CGGTGGTGCA 
GGTGTTGGGT CTGTCGGAGG TGGTGGAGCA GGTGGGATGA CTGGTCATCC GCATTCCATG 
CACCCGGCGG ACATGGTCAG TGACTATATG GCGCATCACC ACAATCCGCA CAGCCACTCC 
CATTCGCACA CCCACTCACT ACCGCACCAC CATAGCAACA GTGCAATATC TGGACACCAC 
CAGGCATCCG CCGGCGGTTA CAGTTCCAAT TATGCCAATG CCACTCCGCC CTCACATCCG 
CACTCGCATC CACACGCCCA TCCGCACCAG AGCCTCGGCT ATTACGTCCA CCACGCCCCC 
GAATTCATAT CCGCAGGAGC GGTGCACTCC GATCCCACGA ACGGATACGG ACCGGCGGCA 
AACGTTCCAA ATACGAGCAA TGGCGGTGGT GGCGGAGGAA GTGGCGCAGT GCTCGGAGGT 
GGCGCAGTGG GTGGATCGGC AAATGGATAT TACGGCGGGT ACGGTGGGGG TTATGGGACG 
GCGAACGGAA GTGTGGGCAG CACCCACTCC CAAGGACACT CGCCGCACTC CCAGATGATG 
GATCTGCCCC TTCAGTGCAG CTCCACGGAA CCACCGACGA ACACGGCGCT GGGACTGCAG 
GAATTAGGCC TAAAACTAGA AAAACGCATA GAAGAAGCTG TACCTGCCGG ACAACAACTG 
CAAGAGCTAG GGATGCGATT GCGGTGTGAT GATATGGGAT CCGAAAACGA CGATATGTCA 
GAGGAGGATC GGCTCATGCT GGATCGATCT CCAGACGAGC TGGGCTCCAA CGACAACGAC 
GACGACCTCG GGGACTCAGA CAGCGATGAG GATCTGATGG CGGAGACGAC CGATGGCGAA 
CGGATCATCT ACCCCTGGAT GAAGAAGATC CATGTGGCGG GAGTTGCGAA CGGGTCTTAC 
CAGCCGGGAA TGGAGCCAAA ACGCCAACGC ACCGCCTACA CACGCCATCA GATCCTGGAA 
CTGGAAAAGG AGTTCCACTA CAACCGCTAC CTGACGCGTC GGCGGCGCAT CGAGATTGCC 
CATACGTTAG TTCTCTCGGA GCGGCAGATC AAGATCTGGT TCCAGAACAG GCGCATGAAG 
TGGAAGAAGG ACAACAAGCT GCCCAACACC AAGAACGTGC GCAAGAAGAC GGTGGACGCC 
AACGGCAACC CAACACCGGT AGCGAAGAAA CCCACCAAGC GGGCCGCCTC CAAAAAGCAG 
CAGCAAGCGC AGCAGCAGCA GCAGTCGCAG CAGCAGCAGA CGCAGCAGAC TCCGGTGATG 
AATGAGTGCA TTCGTTCCGA CAGTTTGGAG AGTATCGGTG ACGTCAGCTC GTCCCTGGGC 
AATCCGCCCT ATATACCGGC GGCACCTGAG ACGACCAGCT CTTATCCGGG ATCTCAGCAG 
CACCTCAGCA ATAACAACAA CAATGGCAGC GGCAATAACA ATAATAACAA CAACAACAAC 
AACAGCAACC TCAATAACAA TAACAATAAC AATCAAATGG GTCACACGAA TCTGCATGGG 
CACCTTCAAC AGCAACAATC CGATCTCATG ACCAATCTTC AGCTACACAT CAAGCAGGAC 
TACGATCTGA CGGCCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477201.1
CDS	490..2250
Translation	MSSFLMGYPHAPHHVQSPMSMGNGLDPKFPPLADDYHHYNGHYSMTASTGHMSGAVGGGAGVGSVGGGGAGGMTGHPHSMHPADMVSDYMAHHHNPHSHSHSHTHSLPHHHSNSAISGHHQASAGGYSSNYANATPPSHPHSHPHAHPHQSLGYYVHHAPEFISAGAVHSDPTNGYGPAANVPNTSNGGGGGGSGAVLGGGAVGGSANGYYGGYGGGYGTANGSVGSTHSQGHSPHSQMMDLPLQCSSTEPPTNTALGLQELGLKLEKRIEEAVPAGQQLQELGMRLRCDDMGSENDDMSEEDRLMLDRSPDELGSNDNDDDLGDSDSDEDLMAETTDGERIIYPWMKKIHVAGVANGSYQPGMEPKRQRTAYTRHQILELEKEFHYNRYLTRRRRIEIAHTLVLSERQIKIWFQNRRMKWKKDNKLPNTKNVRKKTVDANGNPTPVAKKPTKRAASKKQQQAQQQQQSQQQQTQQTPVMNECIRSDSLESIGDVSSSLGNPPYIPAAPETTSSYPGSQQHLSNNNNNGSGNNNNNNNNNNSNLNNNNNNNQMGHTNLHGHLQQQQSDLMTNLQLHIKQDYDLTAL

Target ORF information:

RefSeq Version	NM_057853.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deformed (Dfd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057853.3

ORF Insert Sequence:

ATGAGCTCTT TTCTCATGGG TTATCCGCAT GCACCACATC ATGTCCAGAG TCCCATGTCC 
ATGGGCAATG GCTTGGACCC AAAGTTTCCG CCGTTGGCCG ATGACTATCA TCACTACAAT 
GGACACTATT CGATGACCGC ATCTACGGGT CATATGAGTG GTGCCGTCGG CGGTGGTGCA 
GGTGTTGGGT CTGTCGGAGG TGGTGGAGCA GGTGGGATGA CTGGTCATCC GCATTCCATG 
CACCCGGCGG ACATGGTCAG TGACTATATG GCGCATCACC ACAATCCGCA CAGCCACTCC 
CATTCGCACA CCCACTCACT ACCGCACCAC CATAGCAACA GTGCAATATC TGGACACCAC 
CAGGCATCCG CCGGCGGTTA CAGTTCCAAT TATGCCAATG CCACTCCGCC CTCACATCCG 
CACTCGCATC CACACGCCCA TCCGCACCAG AGCCTCGGCT ATTACGTCCA CCACGCCCCC 
GAATTCATAT CCGCAGGAGC GGTGCACTCC GATCCCACGA ACGGATACGG ACCGGCGGCA 
AACGTTCCAA ATACGAGCAA TGGCGGTGGT GGCGGAGGAA GTGGCGCAGT GCTCGGAGGT 
GGCGCAGTGG GTGGATCGGC AAATGGATAT TACGGCGGGT ACGGTGGGGG TTATGGGACG 
GCGAACGGAA GTGTGGGCAG CACCCACTCC CAAGGACACT CGCCGCACTC CCAGATGATG 
GATCTGCCCC TTCAGTGCAG CTCCACGGAA CCACCGACGA ACACGGCGCT GGGACTGCAG 
GAATTAGGCC TAAAACTAGA AAAACGCATA GAAGAAGCTG TACCTGCCGG ACAACAACTG 
CAAGAGCTAG GGATGCGATT GCGGTGTGAT GATATGGGAT CCGAAAACGA CGATATGTCA 
GAGGAGGATC GGCTCATGCT GGATCGATCT CCAGACGAGC TGGGCTCCAA CGACAACGAC 
GACGACCTCG GGGACTCAGA CAGCGATGAG GATCTGATGG CGGAGACGAC CGATGGCGAA 
CGGATCATCT ACCCCTGGAT GAAGAAGATC CATGTGGCGG GAGTTGCGAA CGGGTCTTAC 
CAGCCGGGAA TGGAGCCAAA ACGCCAACGC ACCGCCTACA CACGCCATCA GATCCTGGAA 
CTGGAAAAGG AGTTCCACTA CAACCGCTAC CTGACGCGTC GGCGGCGCAT CGAGATTGCC 
CATACGTTAG TTCTCTCGGA GCGGCAGATC AAGATCTGGT TCCAGAACAG GCGCATGAAG 
TGGAAGAAGG ACAACAAGCT GCCCAACACC AAGAACGTGC GCAAGAAGAC GGTGGACGCC 
AACGGCAACC CAACACCGGT AGCGAAGAAA CCCACCAAGC GGGCCGCCTC CAAAAAGCAG 
CAGCAAGCGC AGCAGCAGCA GCAGTCGCAG CAGCAGCAGA CGCAGCAGAC TCCGGTGATG 
AATGAGTGCA TTCGTTCCGA CAGTTTGGAG AGTATCGGTG ACGTCAGCTC GTCCCTGGGC 
AATCCGCCCT ATATACCGGC GGCACCTGAG ACGACCAGCT CTTATCCGGG ATCTCAGCAG 
CACCTCAGCA ATAACAACAA CAATGGCAGC GGCAATAACA ATAATAACAA CAACAACAAC 
AACAGCAACC TCAATAACAA TAACAATAAC AATCAAATGG GTCACACGAA TCTGCATGGG 
CACCTTCAAC AGCAACAATC CGATCTCATG ACCAATCTTC AGCTACACAT CAAGCAGGAC 
TACGATCTGA CGGCCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dfd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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