agt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	agt
Entrez Gene ID	40816
Full Name	O-6-alkylguanine-DNA alkyltransferase
Synonyms	Agt,BG:DS00004.10,CG1303,DmAGT,Dmel\CG1303
General protein information	Preferred Names O-6-alkylguanine-DNA alkyltransferase Names CG1303 gene product from transcript CG1303-RB CG1303-PA CG1303-PB agt-PA agt-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84A1-84A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_058018.3	NP_477366.1	O-6-alkylguanine-DNA alkyltransferase, isoform A
NM_001316493.1	NP_001303422.1	O-6-alkylguanine-DNA alkyltransferase, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following agt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the agt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04106	NM_058018.3 Latest version!	Drosophila melanogaster O-6-alkylguanine-DNA alkyltransferase (agt), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa04106	NM_001316493.1 Latest version!	Drosophila melanogaster O-6-alkylguanine-DNA alkyltransferase (agt), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

agt ( NM_058018.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477366.1 Drosophila melanogaster O-6-alkylguanine-DNA alkyltransferase (agt), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04106
Clone ID Related Accession (Same CDS sequence)	NM_058018.3 , NM_001316493.1
Accession Version	NM_058018.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	O-6-alkylguanine-DNA alkyltransferase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_058018.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGATGT GGATTGGCCA AAACACCCAG ATTCGTTTGG TGCCACTAAA GGATCCGCCA 
AAGACCATCC GATACAGTTT CCTGGCGACC AAGTTCGGGC AGATGATGAT GGGAGTGATA 
AACCCAATAT CCGGCGATTC GAAAGACCAA GTCGCAATTG GTGTGCTTCA CTTCGTGATT 
AAGGACAATG TGAGTACCTA CAATGAAGTC CAGGAGCGGT GGCCAAAATC CGAACTCTGG 
AAGGACGATG ATGCGGTCAA GTTGGTGGCG GATAAACTGT TCGAAAGCGA CAAGGAAAAC 
TCGCAGGCGA TTCCCATTGC CATCTATGGA ACCGACTTCC AGCTGTCCGT ATGGCGAGCT 
CTGGTGCATA TGAAACGAGG GGAGACCTGC ACTTACTCCC AACTGGCCGA GCGGATGGGT 
CGTCCAACGG CGGTCAGAGC AGTGGCCAGT GCTGTGGCCA AAAATGAGCT TGCTATCCTC 
ATTCCGTGCC ACCGAGTTGT GTCCCAAAAC GGAGCTTCGA AGTACCACTG GGGCGCAGCC 
CTCAAGCAAC TGCTTCTGGC CGATGAAAAG TCAAAGAACT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477366.1
CDS	79..663
Translation	MTMWIGQNTQIRLVPLKDPPKTIRYSFLATKFGQMMMGVINPISGDSKDQVAIGVLHFVIKDNVSTYNEVQERWPKSELWKDDDAVKLVADKLFESDKENSQAIPIAIYGTDFQLSVWRALVHMKRGETCTYSQLAERMGRPTAVRAVASAVAKNELAILIPCHRVVSQNGASKYHWGAALKQLLLADEKSKNY

Target ORF information:

RefSeq Version	NM_058018.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster O-6-alkylguanine-DNA alkyltransferase (agt), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058018.3

ORF Insert Sequence:

ATGACGATGT GGATTGGCCA AAACACCCAG ATTCGTTTGG TGCCACTAAA GGATCCGCCA 
AAGACCATCC GATACAGTTT CCTGGCGACC AAGTTCGGGC AGATGATGAT GGGAGTGATA 
AACCCAATAT CCGGCGATTC GAAAGACCAA GTCGCAATTG GTGTGCTTCA CTTCGTGATT 
AAGGACAATG TGAGTACCTA CAATGAAGTC CAGGAGCGGT GGCCAAAATC CGAACTCTGG 
AAGGACGATG ATGCGGTCAA GTTGGTGGCG GATAAACTGT TCGAAAGCGA CAAGGAAAAC 
TCGCAGGCGA TTCCCATTGC CATCTATGGA ACCGACTTCC AGCTGTCCGT ATGGCGAGCT 
CTGGTGCATA TGAAACGAGG GGAGACCTGC ACTTACTCCC AACTGGCCGA GCGGATGGGT 
CGTCCAACGG CGGTCAGAGC AGTGGCCAGT GCTGTGGCCA AAAATGAGCT TGCTATCCTC 
ATTCCGTGCC ACCGAGTTGT GTCCCAAAAC GGAGCTTCGA AGTACCACTG GGGCGCAGCC 
CTCAAGCAAC TGCTTCTGGC CGATGAAAAG TCAAAGAACT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agt ( NM_001316493.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303422.1 Drosophila melanogaster O-6-alkylguanine-DNA alkyltransferase (agt), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04106
Clone ID Related Accession (Same CDS sequence)	NM_058018.3 , NM_001316493.1
Accession Version	NM_001316493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	O-6-alkylguanine-DNA alkyltransferase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGATGT GGATTGGCCA AAACACCCAG ATTCGTTTGG TGCCACTAAA GGATCCGCCA 
AAGACCATCC GATACAGTTT CCTGGCGACC AAGTTCGGGC AGATGATGAT GGGAGTGATA 
AACCCAATAT CCGGCGATTC GAAAGACCAA GTCGCAATTG GTGTGCTTCA CTTCGTGATT 
AAGGACAATG TGAGTACCTA CAATGAAGTC CAGGAGCGGT GGCCAAAATC CGAACTCTGG 
AAGGACGATG ATGCGGTCAA GTTGGTGGCG GATAAACTGT TCGAAAGCGA CAAGGAAAAC 
TCGCAGGCGA TTCCCATTGC CATCTATGGA ACCGACTTCC AGCTGTCCGT ATGGCGAGCT 
CTGGTGCATA TGAAACGAGG GGAGACCTGC ACTTACTCCC AACTGGCCGA GCGGATGGGT 
CGTCCAACGG CGGTCAGAGC AGTGGCCAGT GCTGTGGCCA AAAATGAGCT TGCTATCCTC 
ATTCCGTGCC ACCGAGTTGT GTCCCAAAAC GGAGCTTCGA AGTACCACTG GGGCGCAGCC 
CTCAAGCAAC TGCTTCTGGC CGATGAAAAG TCAAAGAACT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303422.1
CDS	79..663
Translation	MTMWIGQNTQIRLVPLKDPPKTIRYSFLATKFGQMMMGVINPISGDSKDQVAIGVLHFVIKDNVSTYNEVQERWPKSELWKDDDAVKLVADKLFESDKENSQAIPIAIYGTDFQLSVWRALVHMKRGETCTYSQLAERMGRPTAVRAVASAVAKNELAILIPCHRVVSQNGASKYHWGAALKQLLLADEKSKNY

Target ORF information:

RefSeq Version	NM_001316493.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster O-6-alkylguanine-DNA alkyltransferase (agt), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316493.1

ORF Insert Sequence:

ATGACGATGT GGATTGGCCA AAACACCCAG ATTCGTTTGG TGCCACTAAA GGATCCGCCA 
AAGACCATCC GATACAGTTT CCTGGCGACC AAGTTCGGGC AGATGATGAT GGGAGTGATA 
AACCCAATAT CCGGCGATTC GAAAGACCAA GTCGCAATTG GTGTGCTTCA CTTCGTGATT 
AAGGACAATG TGAGTACCTA CAATGAAGTC CAGGAGCGGT GGCCAAAATC CGAACTCTGG 
AAGGACGATG ATGCGGTCAA GTTGGTGGCG GATAAACTGT TCGAAAGCGA CAAGGAAAAC 
TCGCAGGCGA TTCCCATTGC CATCTATGGA ACCGACTTCC AGCTGTCCGT ATGGCGAGCT 
CTGGTGCATA TGAAACGAGG GGAGACCTGC ACTTACTCCC AACTGGCCGA GCGGATGGGT 
CGTCCAACGG CGGTCAGAGC AGTGGCCAGT GCTGTGGCCA AAAATGAGCT TGCTATCCTC 
ATTCCGTGCC ACCGAGTTGT GTCCCAAAAC GGAGCTTCGA AGTACCACTG GGGCGCAGCC 
CTCAAGCAAC TGCTTCTGGC CGATGAAAAG TCAAAGAACT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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agt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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