gpp cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following gpp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gpp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa04082 NM_169143.2
Latest version!
Drosophila melanogaster grappa (gpp), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OFa04082 NM_169142.2
Latest version!
Drosophila melanogaster grappa (gpp), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OFa04082 NM_141398.4
Latest version!
Drosophila melanogaster grappa (gpp), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OFa04083 NM_169144.2
Latest version!
Drosophila melanogaster grappa (gpp), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OFa07560 NM_001275387.1
Latest version!
Drosophila melanogaster grappa (gpp), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa04082
    Clone ID Related Accession (Same CDS sequence) NM_169143.2 , NM_141398.4 , NM_169142.2
    Accession Version NM_169143.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product grappa, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_169143.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
    ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC
    GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC
    GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC
    GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC
    GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT
    TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG
    GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC
    AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC
    GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT
    GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC
    GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA
    TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG
    GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG
    CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC
    GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG
    CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC
    AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA
    AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC
    CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG
    CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG
    CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC
    AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC
    AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT
    TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC
    AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA
    CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC
    AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC
    GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT
    GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA
    CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG
    TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG
    TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC
    CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC
    TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA
    AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA
    CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC
    CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA
    AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT
    CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC
    CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA
    AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG
    CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG
    ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG
    GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG
    CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA
    GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA
    GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA
    CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA
    ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG
    AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC
    TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC
    AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA
    TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC
    AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC
    CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT
    CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG
    GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT
    CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC
    CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA
    CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC
    CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC
    CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG
    CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG
    AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT
    CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA
    AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG
    CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT
    CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA
    ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC
    ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG
    CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA
    TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT
    GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA
    AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC
    TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC
    AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA
    GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT
    CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG
    CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA
    CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA
    AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG
    CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG
    CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT
    CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA
    AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC
    AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG
    CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA
    CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG
    GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG TTAACGATTT GGCAACGCAA
    CGTACTATAA CTAATTACGA TCCCAGACGT CGCCTTCGCA CGACTTTGTC TGGCCCAACG
    AAACTATCAG CTGCCCATAG TAATCAAAAT CTGAACGGAT ATGTTATGGC CGATAGTAGT
    AGTAGTTGCC CCACAATACC CCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_731084.1
    CDS1464..7010
    Translation

    Target ORF information:

    RefSeq Version NM_169143.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster grappa (gpp), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169143.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
    ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC
    GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC
    GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC
    GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC
    GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT
    TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG
    GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC
    AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC
    GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT
    GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC
    GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA
    TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG
    GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG
    CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC
    GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG
    CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC
    AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA
    AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC
    CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG
    CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG
    CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC
    AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC
    AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT
    TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC
    AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA
    CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC
    AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC
    GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT
    GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA
    CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG
    TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG
    TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC
    CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC
    TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA
    AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA
    CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC
    CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA
    AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT
    CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC
    CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA
    AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG
    CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG
    ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG
    GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG
    CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA
    GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA
    GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA
    CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA
    ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG
    AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC
    TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC
    AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA
    TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC
    AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC
    CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT
    CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG
    GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT
    CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC
    CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA
    CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC
    CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC
    CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG
    CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG
    AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT
    CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA
    AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG
    CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT
    CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA
    ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC
    ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG
    CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA
    TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT
    GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA
    AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC
    TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC
    AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA
    GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT
    CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG
    CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA
    CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA
    AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG
    CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG
    CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT
    CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA
    AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC
    AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG
    CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA
    CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG
    GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG TTAACGATTT GGCAACGCAA
    CGTACTATAA CTAATTACGA TCCCAGACGT CGCCTTCGCA CGACTTTGTC TGGCCCAACG
    AAACTATCAG CTGCCCATAG TAATCAAAAT CTGAACGGAT ATGTTATGGC CGATAGTAGT
    AGTAGTTGCC CCACAATACC CCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04082
    Clone ID Related Accession (Same CDS sequence) NM_169143.2 , NM_141398.4 , NM_169142.2
    Accession Version NM_141398.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product grappa, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_141398.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
    ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC
    GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC
    GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC
    GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC
    GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT
    TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG
    GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC
    AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC
    GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT
    GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC
    GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA
    TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG
    GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG
    CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC
    GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG
    CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC
    AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA
    AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC
    CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG
    CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG
    CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC
    AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC
    AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT
    TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC
    AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA
    CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC
    AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC
    GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT
    GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA
    CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG
    TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG
    TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC
    CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC
    TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA
    AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA
    CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC
    CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA
    AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT
    CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC
    CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA
    AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG
    CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG
    ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG
    GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG
    CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA
    GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA
    GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA
    CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA
    ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG
    AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC
    TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC
    AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA
    TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC
    AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC
    CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT
    CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG
    GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT
    CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC
    CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA
    CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC
    CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC
    CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG
    CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG
    AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT
    CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA
    AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG
    CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT
    CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA
    ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC
    ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG
    CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA
    TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT
    GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA
    AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC
    TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC
    AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA
    GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT
    CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG
    CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA
    CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA
    AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG
    CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG
    CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT
    CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA
    AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC
    AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG
    CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA
    CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG
    GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG TTAACGATTT GGCAACGCAA
    CGTACTATAA CTAATTACGA TCCCAGACGT CGCCTTCGCA CGACTTTGTC TGGCCCAACG
    AAACTATCAG CTGCCCATAG TAATCAAAAT CTGAACGGAT ATGTTATGGC CGATAGTAGT
    AGTAGTTGCC CCACAATACC CCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_649655.1
    CDS1464..7010
    Translation

    Target ORF information:

    RefSeq Version NM_141398.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster grappa (gpp), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_141398.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
    ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC
    GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC
    GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC
    GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC
    GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT
    TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG
    GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC
    AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC
    GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT
    GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC
    GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA
    TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG
    GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG
    CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC
    GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG
    CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC
    AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA
    AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC
    CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG
    CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG
    CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC
    AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC
    AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT
    TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC
    AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA
    CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC
    AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC
    GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT
    GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA
    CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG
    TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG
    TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC
    CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC
    TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA
    AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA
    CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC
    CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA
    AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT
    CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC
    CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA
    AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG
    CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG
    ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG
    GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG
    CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA
    GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA
    GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA
    CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA
    ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG
    AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC
    TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC
    AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA
    TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC
    AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC
    CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT
    CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG
    GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT
    CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC
    CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA
    CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC
    CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC
    CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG
    CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG
    AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT
    CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA
    AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG
    CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT
    CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA
    ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC
    ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG
    CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA
    TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT
    GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA
    AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC
    TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC
    AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA
    GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT
    CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG
    CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA
    CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA
    AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG
    CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG
    CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT
    CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA
    AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC
    AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG
    CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA
    CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG
    GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG TTAACGATTT GGCAACGCAA
    CGTACTATAA CTAATTACGA TCCCAGACGT CGCCTTCGCA CGACTTTGTC TGGCCCAACG
    AAACTATCAG CTGCCCATAG TAATCAAAAT CTGAACGGAT ATGTTATGGC CGATAGTAGT
    AGTAGTTGCC CCACAATACC CCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04082
    Clone ID Related Accession (Same CDS sequence) NM_169143.2 , NM_141398.4 , NM_169142.2
    Accession Version NM_169142.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product grappa, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_169142.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
    ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC
    GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC
    GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC
    GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC
    GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT
    TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG
    GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC
    AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC
    GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT
    GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC
    GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA
    TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG
    GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG
    CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC
    GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG
    CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC
    AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA
    AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC
    CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG
    CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG
    CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC
    AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC
    AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT
    TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC
    AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA
    CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC
    AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC
    GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT
    GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA
    CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG
    TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG
    TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC
    CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC
    TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA
    AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA
    CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC
    CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA
    AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT
    CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC
    CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA
    AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG
    CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG
    ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG
    GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG
    CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA
    GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA
    GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA
    CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA
    ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG
    AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC
    TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC
    AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA
    TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC
    AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC
    CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT
    CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG
    GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT
    CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC
    CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA
    CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC
    CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC
    CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG
    CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG
    AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT
    CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA
    AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG
    CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT
    CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA
    ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC
    ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG
    CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA
    TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT
    GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA
    AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC
    TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC
    AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA
    GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT
    CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG
    CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA
    CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA
    AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG
    CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG
    CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT
    CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA
    AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC
    AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG
    CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA
    CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG
    GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG TTAACGATTT GGCAACGCAA
    CGTACTATAA CTAATTACGA TCCCAGACGT CGCCTTCGCA CGACTTTGTC TGGCCCAACG
    AAACTATCAG CTGCCCATAG TAATCAAAAT CTGAACGGAT ATGTTATGGC CGATAGTAGT
    AGTAGTTGCC CCACAATACC CCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_731083.1
    CDS1850..7396
    Translation

    Target ORF information:

    RefSeq Version NM_169142.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster grappa (gpp), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169142.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
    ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC
    GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC
    GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC
    GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC
    GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT
    TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG
    GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC
    AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC
    GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT
    GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC
    GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA
    TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG
    GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG
    CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC
    GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG
    CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC
    AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA
    AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC
    CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG
    CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG
    CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC
    AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC
    AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT
    TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC
    AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA
    CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC
    AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC
    GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT
    GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA
    CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG
    TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG
    TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC
    CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC
    TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA
    AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA
    CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC
    CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA
    AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT
    CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC
    CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA
    AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG
    CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG
    ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG
    GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG
    CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA
    GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA
    GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA
    CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA
    ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG
    AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC
    TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC
    AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA
    TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC
    AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC
    CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT
    CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG
    GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT
    CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC
    CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA
    CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC
    CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC
    CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG
    CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG
    AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT
    CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA
    AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG
    CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT
    CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA
    ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC
    ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG
    CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA
    TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT
    GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA
    AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC
    TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC
    AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA
    GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT
    CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG
    CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA
    CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA
    AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG
    CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG
    CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT
    CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA
    AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC
    AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG
    CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA
    CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG
    GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG TTAACGATTT GGCAACGCAA
    CGTACTATAA CTAATTACGA TCCCAGACGT CGCCTTCGCA CGACTTTGTC TGGCCCAACG
    AAACTATCAG CTGCCCATAG TAATCAAAAT CTGAACGGAT ATGTTATGGC CGATAGTAGT
    AGTAGTTGCC CCACAATACC CCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04083
    Clone ID Related Accession (Same CDS sequence) NM_169144.2
    Accession Version NM_169144.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product grappa, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_169144.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
    ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC
    GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC
    GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC
    GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC
    GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT
    TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG
    GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC
    AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC
    GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT
    GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC
    GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA
    TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG
    GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG
    CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC
    GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG
    CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC
    AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA
    AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC
    CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG
    CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG
    CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC
    AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC
    AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT
    TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC
    AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA
    CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC
    AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC
    GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT
    GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA
    CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG
    TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG
    TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC
    CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC
    TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA
    AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA
    CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC
    CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA
    AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT
    CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC
    CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA
    AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG
    CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG
    ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG
    GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG
    CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA
    GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA
    GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA
    CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA
    ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG
    AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC
    TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC
    AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA
    TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC
    AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC
    CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT
    CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG
    GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT
    CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC
    CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA
    CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC
    CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC
    CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG
    CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG
    AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT
    CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA
    AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG
    CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT
    CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA
    ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC
    ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG
    CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA
    TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT
    GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA
    AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC
    TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC
    AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA
    GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT
    CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG
    CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA
    CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA
    AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG
    CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG
    CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT
    CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA
    AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC
    AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG
    CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA
    CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG
    GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG ACGATGTTAT TGCCGCTGAT
    GATGAGACCC ACTGGCAGCA TCGAGTCAGC TCTGGTTTCG ACCGCCTCGT GGCCTTCGCC
    AGCACTGAAT TGGATAAGAC CAGGCGAAGC ATCGATGGCG ATACCGTGCC GGCCTCGATT
    AGTTGCAACA CGTCTCCGGA CTCGGGCATT ACGCACAGCA GCAGCAACTC AGATGCAGTG
    CGCACATTTT TGTCCACCTC GTCCAGCTCC TCGCAGCTGG AGCTGCCCAT AGGAAGCGGT
    GGTAGTAGCT GCAACAGCCT CTACAACCAT CATGCCCAGC ACAACAACAT TGGCATTGGC
    GGCAGCGGAG GCAGCCACCC ACATCAGCAA CAGCAACAGT TGCAGGCGCA GCACCAACAG
    CAGCAGCACC ATCTTTCCGA TCACATGAGC GACAGTAGCA TGAAATCCTC GGCATCCTCC
    TCGCTGCATG CTGGTAGCTC CCTTAAGACC GTGTCGCCAG TGGATCCCAG TGCCATCGAG
    TCGCCGCCAC TCTCGGACGT TGGATTGCCC AGAACTCCCA GTCCCAGTGC AGCCAGTGTG
    GCCACTCCAC CACTGGCCAG TGGACCACCC GTGTCCGGGG ACTTAGGTGG TATACCTCTG
    AAGTACCAGC GCAAGTCCAA GACCAGTTCG GAAAAGCACT ACAAAAAGAA GTTCTGCGAA
    AGAAACTGGG AGTACGACTG TGATGAACTG CTGGCCTACT CCAACTCCAG TGCACCGCCG
    ACGGCAGCAG ATGCAGCGGG CAATGGGCCA CTTAACTTGG ATGCTCCCAA TACTGTGGGC
    GGAGCACCTG CACCGCTCTT GGGAAAATCG GGAAAGTCAC CCAAGGAAAA GTCTTCACCG
    CACCACACGG GCTTAAACCA TCAGCAGCAG CACCATCACA AGTCATCCAA ATTTCGGCCA
    AAGGGCAAGG ACTGGGACTG GTCAATGGAC AGTCGGAGCC AAACGGGCGA GCTGCTGGCC
    GCCAGTAACA ACATGAACAT TAACAACAAC TCAGCCACCA CAACGAGTAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_731085.2
    CDS1464..7877
    Translation

    Target ORF information:

    RefSeq Version NM_169144.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster grappa (gpp), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169144.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
    ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC
    GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC
    GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC
    GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC
    GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT
    TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG
    GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC
    AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC
    GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT
    GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC
    GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA
    TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG
    GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG
    CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC
    GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG
    CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC
    AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA
    AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC
    CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG
    CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG
    CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC
    AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC
    AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT
    TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC
    AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA
    CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC
    AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC
    GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT
    GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA
    CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG
    TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG
    TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC
    CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC
    TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA
    AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA
    CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC
    CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA
    AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT
    CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC
    CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA
    AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG
    CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG
    ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG
    GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG
    CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA
    GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA
    GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA
    CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA
    ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG
    AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC
    TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC
    AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA
    TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC
    AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC
    CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT
    CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG
    GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT
    CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC
    CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA
    CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC
    CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC
    CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG
    CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG
    AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT
    CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA
    AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG
    CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT
    CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA
    ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC
    ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG
    CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA
    TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT
    GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA
    AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC
    TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC
    AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA
    GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT
    CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG
    CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA
    CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA
    AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG
    CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG
    CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT
    CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA
    AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC
    AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG
    CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA
    CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG
    GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG ACGATGTTAT TGCCGCTGAT
    GATGAGACCC ACTGGCAGCA TCGAGTCAGC TCTGGTTTCG ACCGCCTCGT GGCCTTCGCC
    AGCACTGAAT TGGATAAGAC CAGGCGAAGC ATCGATGGCG ATACCGTGCC GGCCTCGATT
    AGTTGCAACA CGTCTCCGGA CTCGGGCATT ACGCACAGCA GCAGCAACTC AGATGCAGTG
    CGCACATTTT TGTCCACCTC GTCCAGCTCC TCGCAGCTGG AGCTGCCCAT AGGAAGCGGT
    GGTAGTAGCT GCAACAGCCT CTACAACCAT CATGCCCAGC ACAACAACAT TGGCATTGGC
    GGCAGCGGAG GCAGCCACCC ACATCAGCAA CAGCAACAGT TGCAGGCGCA GCACCAACAG
    CAGCAGCACC ATCTTTCCGA TCACATGAGC GACAGTAGCA TGAAATCCTC GGCATCCTCC
    TCGCTGCATG CTGGTAGCTC CCTTAAGACC GTGTCGCCAG TGGATCCCAG TGCCATCGAG
    TCGCCGCCAC TCTCGGACGT TGGATTGCCC AGAACTCCCA GTCCCAGTGC AGCCAGTGTG
    GCCACTCCAC CACTGGCCAG TGGACCACCC GTGTCCGGGG ACTTAGGTGG TATACCTCTG
    AAGTACCAGC GCAAGTCCAA GACCAGTTCG GAAAAGCACT ACAAAAAGAA GTTCTGCGAA
    AGAAACTGGG AGTACGACTG TGATGAACTG CTGGCCTACT CCAACTCCAG TGCACCGCCG
    ACGGCAGCAG ATGCAGCGGG CAATGGGCCA CTTAACTTGG ATGCTCCCAA TACTGTGGGC
    GGAGCACCTG CACCGCTCTT GGGAAAATCG GGAAAGTCAC CCAAGGAAAA GTCTTCACCG
    CACCACACGG GCTTAAACCA TCAGCAGCAG CACCATCACA AGTCATCCAA ATTTCGGCCA
    AAGGGCAAGG ACTGGGACTG GTCAATGGAC AGTCGGAGCC AAACGGGCGA GCTGCTGGCC
    GCCAGTAACA ACATGAACAT TAACAACAAC TCAGCCACCA CAACGAGTAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa07560
    Clone ID Related Accession (Same CDS sequence) NM_001275387.1
    Accession Version NM_001275387.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5697bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product grappa, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
    ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC
    GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC
    GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC
    GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC
    GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT
    TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG
    GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC
    AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC
    GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT
    GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC
    GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA
    TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG
    GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG
    CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC
    GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG
    CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC
    AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA
    AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC
    CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG
    CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG
    CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC
    AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC
    AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT
    TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC
    AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA
    CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC
    AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC
    GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT
    GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA
    CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG
    TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG
    TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC
    CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC
    TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA
    AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA
    CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC
    CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA
    AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT
    CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC
    CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA
    AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG
    CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG
    ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG
    GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG
    CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA
    GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA
    GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA
    CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA
    ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG
    AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC
    TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC
    AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA
    TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC
    AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC
    CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT
    CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG
    GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT
    CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC
    CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA
    CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC
    CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC
    CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG
    CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG
    AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT
    CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA
    AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG
    CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT
    CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA
    ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC
    ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG
    CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA
    TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT
    GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA
    AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC
    TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC
    AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA
    GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT
    CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG
    CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA
    CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA
    AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG
    CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG
    CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT
    CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA
    AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC
    AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG
    CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA
    CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG
    GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG GTAAGCTATA CGTAGACCAA
    GCAAAAAACT CGACCAGGGT ATCTGTACCC AAATATGCAC CACAACAAGC ACCACCACCT
    GCACACGTAG CATTCCCGCT AGAGCCACAT ATTCTTCACC AGCACAAATA CCAACGACTA
    GCCACCCAAA CGGTGGCAGA AGATCTTACC ATGAAGCGAA AAGAGCAGGA ATCGGATTCA
    GAACTCGAGC CGGAAGTGGT TGCTGCGTTT GTCGAAGATG GAGGTTCCCA TAGCGGATCC
    TCATCGTCGA GTTCCAGCTC GCACTCAGGA TCTGATTCCA GTTCTGACGT GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262316.1
    CDS1852..7548
    Translation

    Target ORF information:

    RefSeq Version NM_001275387.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster grappa (gpp), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001275387.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
    ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC
    GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC
    GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC
    GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC
    GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT
    TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG
    GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC
    AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC
    GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT
    GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC
    GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA
    TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG
    GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG
    CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC
    GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG
    CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC
    AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA
    AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC
    CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG
    CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG
    CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC
    AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC
    AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT
    TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC
    AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA
    CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC
    AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC
    GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT
    GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA
    CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG
    TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG
    TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC
    CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC
    TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA
    AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA
    CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC
    CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA
    AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT
    CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC
    CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA
    AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG
    CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG
    ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG
    GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG
    CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA
    GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA
    GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA
    CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA
    ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG
    AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC
    TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC
    AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA
    TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC
    AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC
    CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT
    CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG
    GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT
    CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC
    CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA
    CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC
    CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC
    CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG
    CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG
    AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT
    CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA
    AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG
    CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT
    CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA
    ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC
    ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG
    CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA
    TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT
    GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA
    AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC
    TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC
    AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA
    GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT
    CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG
    CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA
    CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA
    AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG
    CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG
    CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT
    CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA
    AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC
    AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG
    CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA
    CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG
    GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG GTAAGCTATA CGTAGACCAA
    GCAAAAAACT CGACCAGGGT ATCTGTACCC AAATATGCAC CACAACAAGC ACCACCACCT
    GCACACGTAG CATTCCCGCT AGAGCCACAT ATTCTTCACC AGCACAAATA CCAACGACTA
    GCCACCCAAA CGGTGGCAGA AGATCTTACC ATGAAGCGAA AAGAGCAGGA ATCGGATTCA
    GAACTCGAGC CGGAAGTGGT TGCTGCGTTT GTCGAAGATG GAGGTTCCCA TAGCGGATCC
    TCATCGTCGA GTTCCAGCTC GCACTCAGGA TCTGATTCCA GTTCTGACGT GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.