Rga cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rga
Entrez Gene ID	40683
Full Name	Regena
Synonyms	CG2138,CG2161,Dmel\CG2161,NOT2,Not2,RGA,l(3)03834,not2
General protein information	Preferred Names Regena Names CG2161 gene product from transcript CG2161-RA CG2161-PA CG2161-PB CG2161-PC Rga-PA Rga-PB Rga-PC regena
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83B5-83B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_079515.3	NP_524239.2	regena, isoform B
NM_001300254.1	NP_001287183.1	regena, isoform C
NM_169080.3	NP_730966.1	regena, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003525	XP_562364.3
Drosophila melanogaster (fruit fly)	Rga	NP_524239.2
Bos taurus (cattle)	CNOT2	NP_001096747.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rga gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rga cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03965	NM_001300254.1 Latest version!	Drosophila melanogaster regena, transcript variant C (Rga), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa03965	NM_079515.3 Latest version!	Drosophila melanogaster regena, transcript variant B (Rga), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa03966	NM_169080.3 Latest version!	Drosophila melanogaster regena, transcript variant A (Rga), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rga ( NM_079515.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524239.2 Drosophila melanogaster regena, transcript variant B (Rga), mRNA.

Price & Availability↑

CloneID	OFa03965
Clone ID Related Accession (Same CDS sequence)	NM_079515.3 , NM_001300254.1
Accession Version	NM_079515.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	regena, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079515.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAATT TAAATTTTCA ACAACCCCCG AGAAGTATAG CGAACGCGGC GTTAGCTGGC 
CGGACAACAG GCGGATTCGG TGGTAGCTCC CTGGCTGGCC ATGTGACGCC CACGTCGGGC 
ATGTTTCAAA CCGACTTCGC CAACTCCTAT CCAGGAACGG CGAACTACGG ACAGGCGCCG 
CAGCAGCAGC AACAACAGCA GCAGCAGCCC CAACTGTCGC CGAACCGGAA TGCCCAGCTG 
TCGGTCGGCG GACCCGCCAT CTCGAGCGGC AACCGCAACG CCAACCTCTT CGGCCAGCGT 
CAGTTTGTGG AGCGGCGAGC CATGCAAGGA TTGGGCAGTG GCCCCATGTC GAACATGGGC 
AACTTCATGC AGACGGGTCG CGGCGGCTAC GGAACGGGCG GAGGCGGTGG CGGTCCTTTG 
AATAACTTCC ACGTGTTCGG CGGCGGCGGT GGTAGCGACA CCTCCACGCC TGCCCTGCTC 
GATCCCACGG AGTTCCCCTC GCTGACGAAT GCGCGCGGCC AGAACGACCA GACACTGCCC 
CAATCGAATC CGCTCCAGCC GCCGGGCAGC AAACCCTATG TTGGCATGGT GAAGCAACCG 
ACGTCAGAGC AATCGGAGTT CACGATGTCT AACGAGGACT TCCCTGCGTT ACCGGGCACC 
CAAAACTCGG ACGGCACAAC GAACGCGGTG GGGAGCGTCG CCGGAACAGG AGGCTCCGGA 
GGTGCGTCCA CAGAGAACCA CTTGGACGGC ACGGAAAAGC CGATGAATTC GATAGTGGTC 
AGCGGATCAG CGAGCGGCAG CAGCGGTTCC AACGTCGGCG TTGTAGGCGG CAATGGTCTC 
GGTGCCGTTG GCAGTGGTAT AGGGGGCTTG GCAGTAGGCG GCGGCGGAGG AGCTGGTAGC 
TCAGGAGGCG GAGGAGTCGG CGGCAATGCA GCCAGCGGCG TTGTCGGCGG GTCCCATGTA 
GGACTGGTGG GCAGTAACAG CGGCATTGGC GGCGTCAACA GCGTACCCAA CAGCAATGCC 
ATGATGGGCG TAGGCGGCGG CCTGGGGAGT GGAAGCGGCT CGTCTGGGAG CGGAGCGGGC 
GGCGAGCATC TGAACGATAA TTCCAGCAAC GATAAACTTG TAAAGAGCGG CGTGCAAACT 
TCGCCAGATG GGAAGGTCAC AAACATACCA GCGACCATGG TGAACAACCA GTTCGGCATG 
GTTGGCCTGC TGACGTTCAT TCGGGCTGCA GAAACGGATC CCAATCTGGT GACGCTCTCC 
TTGGGTACGG ATCTCACGGG TTTAGGCCTG AACCTGAACT CGCAGGAGAG CTTGCATACA 
ACCTTCGCTG GCCCCTTCGT AGCACAGCCC TGCAGAGCAC AAGACGTTGA GTTCAACGTG 
CCACCCGAAT ATCTGATTAA CTTTGCGATA AGAGACAAAC TAACAGCGCC GGTACTGAAA 
AAGCTGCAAG AAGACCTGCT CTTTTTCCTT TTTTACACGA ACATCGGCGA TATGATGCAA 
TTGATGGCAG CTGCTGAATT GCATAGTCGC GAGTGGCGAT ACCACGTGGA GGAGAAAATA 
TGGATAACTC GAATTCCTGG CATTGATCAA TATGAAAAAA ATGGTACAAA AGAGCGCGGC 
ACCTTCTACT ACTTTGATGC GCAAAGTTGG AAACGTTTGT CCAAGGTTTT CCAAATAGAT 
CCCGAGAAAT TAGATAAATG TCCCAATATA AGTGCGTTTA TGAATGGACA GTCTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524239.2
CDS	1047..2786
Translation	MANLNFQQPPRSIANAALAGRTTGGFGGSSLAGHVTPTSGMFQTDFANSYPGTANYGQAPQQQQQQQQQPQLSPNRNAQLSVGGPAISSGNRNANLFGQRQFVERRAMQGLGSGPMSNMGNFMQTGRGGYGTGGGGGGPLNNFHVFGGGGGSDTSTPALLDPTEFPSLTNARGQNDQTLPQSNPLQPPGSKPYVGMVKQPTSEQSEFTMSNEDFPALPGTQNSDGTTNAVGSVAGTGGSGGASTENHLDGTEKPMNSIVVSGSASGSSGSNVGVVGGNGLGAVGSGIGGLAVGGGGGAGSSGGGGVGGNAASGVVGGSHVGLVGSNSGIGGVNSVPNSNAMMGVGGGLGSGSGSSGSGAGGEHLNDNSSNDKLVKSGVQTSPDGKVTNIPATMVNNQFGMVGLLTFIRAAETDPNLVTLSLGTDLTGLGLNLNSQESLHTTFAGPFVAQPCRAQDVEFNVPPEYLINFAIRDKLTAPVLKKLQEDLLFFLFYTNIGDMMQLMAAAELHSREWRYHVEEKIWITRIPGIDQYEKNGTKERGTFYYFDAQSWKRLSKVFQIDPEKLDKCPNISAFMNGQSV

Target ORF information:

RefSeq Version	NM_079515.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster regena, transcript variant B (Rga), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079515.3

ORF Insert Sequence:

ATGGCGAATT TAAATTTTCA ACAACCCCCG AGAAGTATAG CGAACGCGGC GTTAGCTGGC 
CGGACAACAG GCGGATTCGG TGGTAGCTCC CTGGCTGGCC ATGTGACGCC CACGTCGGGC 
ATGTTTCAAA CCGACTTCGC CAACTCCTAT CCAGGAACGG CGAACTACGG ACAGGCGCCG 
CAGCAGCAGC AACAACAGCA GCAGCAGCCC CAACTGTCGC CGAACCGGAA TGCCCAGCTG 
TCGGTCGGCG GACCCGCCAT CTCGAGCGGC AACCGCAACG CCAACCTCTT CGGCCAGCGT 
CAGTTTGTGG AGCGGCGAGC CATGCAAGGA TTGGGCAGTG GCCCCATGTC GAACATGGGC 
AACTTCATGC AGACGGGTCG CGGCGGCTAC GGAACGGGCG GAGGCGGTGG CGGTCCTTTG 
AATAACTTCC ACGTGTTCGG CGGCGGCGGT GGTAGCGACA CCTCCACGCC TGCCCTGCTC 
GATCCCACGG AGTTCCCCTC GCTGACGAAT GCGCGCGGCC AGAACGACCA GACACTGCCC 
CAATCGAATC CGCTCCAGCC GCCGGGCAGC AAACCCTATG TTGGCATGGT GAAGCAACCG 
ACGTCAGAGC AATCGGAGTT CACGATGTCT AACGAGGACT TCCCTGCGTT ACCGGGCACC 
CAAAACTCGG ACGGCACAAC GAACGCGGTG GGGAGCGTCG CCGGAACAGG AGGCTCCGGA 
GGTGCGTCCA CAGAGAACCA CTTGGACGGC ACGGAAAAGC CGATGAATTC GATAGTGGTC 
AGCGGATCAG CGAGCGGCAG CAGCGGTTCC AACGTCGGCG TTGTAGGCGG CAATGGTCTC 
GGTGCCGTTG GCAGTGGTAT AGGGGGCTTG GCAGTAGGCG GCGGCGGAGG AGCTGGTAGC 
TCAGGAGGCG GAGGAGTCGG CGGCAATGCA GCCAGCGGCG TTGTCGGCGG GTCCCATGTA 
GGACTGGTGG GCAGTAACAG CGGCATTGGC GGCGTCAACA GCGTACCCAA CAGCAATGCC 
ATGATGGGCG TAGGCGGCGG CCTGGGGAGT GGAAGCGGCT CGTCTGGGAG CGGAGCGGGC 
GGCGAGCATC TGAACGATAA TTCCAGCAAC GATAAACTTG TAAAGAGCGG CGTGCAAACT 
TCGCCAGATG GGAAGGTCAC AAACATACCA GCGACCATGG TGAACAACCA GTTCGGCATG 
GTTGGCCTGC TGACGTTCAT TCGGGCTGCA GAAACGGATC CCAATCTGGT GACGCTCTCC 
TTGGGTACGG ATCTCACGGG TTTAGGCCTG AACCTGAACT CGCAGGAGAG CTTGCATACA 
ACCTTCGCTG GCCCCTTCGT AGCACAGCCC TGCAGAGCAC AAGACGTTGA GTTCAACGTG 
CCACCCGAAT ATCTGATTAA CTTTGCGATA AGAGACAAAC TAACAGCGCC GGTACTGAAA 
AAGCTGCAAG AAGACCTGCT CTTTTTCCTT TTTTACACGA ACATCGGCGA TATGATGCAA 
TTGATGGCAG CTGCTGAATT GCATAGTCGC GAGTGGCGAT ACCACGTGGA GGAGAAAATA 
TGGATAACTC GAATTCCTGG CATTGATCAA TATGAAAAAA ATGGTACAAA AGAGCGCGGC 
ACCTTCTACT ACTTTGATGC GCAAAGTTGG AAACGTTTGT CCAAGGTTTT CCAAATAGAT 
CCCGAGAAAT TAGATAAATG TCCCAATATA AGTGCGTTTA TGAATGGACA GTCTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rga ( NM_001300254.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287183.1 Drosophila melanogaster regena, transcript variant C (Rga), mRNA.

Price & Availability↑

CloneID	OFa03965
Clone ID Related Accession (Same CDS sequence)	NM_079515.3 , NM_001300254.1
Accession Version	NM_001300254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	regena, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAATT TAAATTTTCA ACAACCCCCG AGAAGTATAG CGAACGCGGC GTTAGCTGGC 
CGGACAACAG GCGGATTCGG TGGTAGCTCC CTGGCTGGCC ATGTGACGCC CACGTCGGGC 
ATGTTTCAAA CCGACTTCGC CAACTCCTAT CCAGGAACGG CGAACTACGG ACAGGCGCCG 
CAGCAGCAGC AACAACAGCA GCAGCAGCCC CAACTGTCGC CGAACCGGAA TGCCCAGCTG 
TCGGTCGGCG GACCCGCCAT CTCGAGCGGC AACCGCAACG CCAACCTCTT CGGCCAGCGT 
CAGTTTGTGG AGCGGCGAGC CATGCAAGGA TTGGGCAGTG GCCCCATGTC GAACATGGGC 
AACTTCATGC AGACGGGTCG CGGCGGCTAC GGAACGGGCG GAGGCGGTGG CGGTCCTTTG 
AATAACTTCC ACGTGTTCGG CGGCGGCGGT GGTAGCGACA CCTCCACGCC TGCCCTGCTC 
GATCCCACGG AGTTCCCCTC GCTGACGAAT GCGCGCGGCC AGAACGACCA GACACTGCCC 
CAATCGAATC CGCTCCAGCC GCCGGGCAGC AAACCCTATG TTGGCATGGT GAAGCAACCG 
ACGTCAGAGC AATCGGAGTT CACGATGTCT AACGAGGACT TCCCTGCGTT ACCGGGCACC 
CAAAACTCGG ACGGCACAAC GAACGCGGTG GGGAGCGTCG CCGGAACAGG AGGCTCCGGA 
GGTGCGTCCA CAGAGAACCA CTTGGACGGC ACGGAAAAGC CGATGAATTC GATAGTGGTC 
AGCGGATCAG CGAGCGGCAG CAGCGGTTCC AACGTCGGCG TTGTAGGCGG CAATGGTCTC 
GGTGCCGTTG GCAGTGGTAT AGGGGGCTTG GCAGTAGGCG GCGGCGGAGG AGCTGGTAGC 
TCAGGAGGCG GAGGAGTCGG CGGCAATGCA GCCAGCGGCG TTGTCGGCGG GTCCCATGTA 
GGACTGGTGG GCAGTAACAG CGGCATTGGC GGCGTCAACA GCGTACCCAA CAGCAATGCC 
ATGATGGGCG TAGGCGGCGG CCTGGGGAGT GGAAGCGGCT CGTCTGGGAG CGGAGCGGGC 
GGCGAGCATC TGAACGATAA TTCCAGCAAC GATAAACTTG TAAAGAGCGG CGTGCAAACT 
TCGCCAGATG GGAAGGTCAC AAACATACCA GCGACCATGG TGAACAACCA GTTCGGCATG 
GTTGGCCTGC TGACGTTCAT TCGGGCTGCA GAAACGGATC CCAATCTGGT GACGCTCTCC 
TTGGGTACGG ATCTCACGGG TTTAGGCCTG AACCTGAACT CGCAGGAGAG CTTGCATACA 
ACCTTCGCTG GCCCCTTCGT AGCACAGCCC TGCAGAGCAC AAGACGTTGA GTTCAACGTG 
CCACCCGAAT ATCTGATTAA CTTTGCGATA AGAGACAAAC TAACAGCGCC GGTACTGAAA 
AAGCTGCAAG AAGACCTGCT CTTTTTCCTT TTTTACACGA ACATCGGCGA TATGATGCAA 
TTGATGGCAG CTGCTGAATT GCATAGTCGC GAGTGGCGAT ACCACGTGGA GGAGAAAATA 
TGGATAACTC GAATTCCTGG CATTGATCAA TATGAAAAAA ATGGTACAAA AGAGCGCGGC 
ACCTTCTACT ACTTTGATGC GCAAAGTTGG AAACGTTTGT CCAAGGTTTT CCAAATAGAT 
CCCGAGAAAT TAGATAAATG TCCCAATATA AGTGCGTTTA TGAATGGACA GTCTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287183.1
CDS	745..2484
Translation	MANLNFQQPPRSIANAALAGRTTGGFGGSSLAGHVTPTSGMFQTDFANSYPGTANYGQAPQQQQQQQQQPQLSPNRNAQLSVGGPAISSGNRNANLFGQRQFVERRAMQGLGSGPMSNMGNFMQTGRGGYGTGGGGGGPLNNFHVFGGGGGSDTSTPALLDPTEFPSLTNARGQNDQTLPQSNPLQPPGSKPYVGMVKQPTSEQSEFTMSNEDFPALPGTQNSDGTTNAVGSVAGTGGSGGASTENHLDGTEKPMNSIVVSGSASGSSGSNVGVVGGNGLGAVGSGIGGLAVGGGGGAGSSGGGGVGGNAASGVVGGSHVGLVGSNSGIGGVNSVPNSNAMMGVGGGLGSGSGSSGSGAGGEHLNDNSSNDKLVKSGVQTSPDGKVTNIPATMVNNQFGMVGLLTFIRAAETDPNLVTLSLGTDLTGLGLNLNSQESLHTTFAGPFVAQPCRAQDVEFNVPPEYLINFAIRDKLTAPVLKKLQEDLLFFLFYTNIGDMMQLMAAAELHSREWRYHVEEKIWITRIPGIDQYEKNGTKERGTFYYFDAQSWKRLSKVFQIDPEKLDKCPNISAFMNGQSV

Target ORF information:

RefSeq Version	NM_001300254.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster regena, transcript variant C (Rga), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300254.1

ORF Insert Sequence:

ATGGCGAATT TAAATTTTCA ACAACCCCCG AGAAGTATAG CGAACGCGGC GTTAGCTGGC 
CGGACAACAG GCGGATTCGG TGGTAGCTCC CTGGCTGGCC ATGTGACGCC CACGTCGGGC 
ATGTTTCAAA CCGACTTCGC CAACTCCTAT CCAGGAACGG CGAACTACGG ACAGGCGCCG 
CAGCAGCAGC AACAACAGCA GCAGCAGCCC CAACTGTCGC CGAACCGGAA TGCCCAGCTG 
TCGGTCGGCG GACCCGCCAT CTCGAGCGGC AACCGCAACG CCAACCTCTT CGGCCAGCGT 
CAGTTTGTGG AGCGGCGAGC CATGCAAGGA TTGGGCAGTG GCCCCATGTC GAACATGGGC 
AACTTCATGC AGACGGGTCG CGGCGGCTAC GGAACGGGCG GAGGCGGTGG CGGTCCTTTG 
AATAACTTCC ACGTGTTCGG CGGCGGCGGT GGTAGCGACA CCTCCACGCC TGCCCTGCTC 
GATCCCACGG AGTTCCCCTC GCTGACGAAT GCGCGCGGCC AGAACGACCA GACACTGCCC 
CAATCGAATC CGCTCCAGCC GCCGGGCAGC AAACCCTATG TTGGCATGGT GAAGCAACCG 
ACGTCAGAGC AATCGGAGTT CACGATGTCT AACGAGGACT TCCCTGCGTT ACCGGGCACC 
CAAAACTCGG ACGGCACAAC GAACGCGGTG GGGAGCGTCG CCGGAACAGG AGGCTCCGGA 
GGTGCGTCCA CAGAGAACCA CTTGGACGGC ACGGAAAAGC CGATGAATTC GATAGTGGTC 
AGCGGATCAG CGAGCGGCAG CAGCGGTTCC AACGTCGGCG TTGTAGGCGG CAATGGTCTC 
GGTGCCGTTG GCAGTGGTAT AGGGGGCTTG GCAGTAGGCG GCGGCGGAGG AGCTGGTAGC 
TCAGGAGGCG GAGGAGTCGG CGGCAATGCA GCCAGCGGCG TTGTCGGCGG GTCCCATGTA 
GGACTGGTGG GCAGTAACAG CGGCATTGGC GGCGTCAACA GCGTACCCAA CAGCAATGCC 
ATGATGGGCG TAGGCGGCGG CCTGGGGAGT GGAAGCGGCT CGTCTGGGAG CGGAGCGGGC 
GGCGAGCATC TGAACGATAA TTCCAGCAAC GATAAACTTG TAAAGAGCGG CGTGCAAACT 
TCGCCAGATG GGAAGGTCAC AAACATACCA GCGACCATGG TGAACAACCA GTTCGGCATG 
GTTGGCCTGC TGACGTTCAT TCGGGCTGCA GAAACGGATC CCAATCTGGT GACGCTCTCC 
TTGGGTACGG ATCTCACGGG TTTAGGCCTG AACCTGAACT CGCAGGAGAG CTTGCATACA 
ACCTTCGCTG GCCCCTTCGT AGCACAGCCC TGCAGAGCAC AAGACGTTGA GTTCAACGTG 
CCACCCGAAT ATCTGATTAA CTTTGCGATA AGAGACAAAC TAACAGCGCC GGTACTGAAA 
AAGCTGCAAG AAGACCTGCT CTTTTTCCTT TTTTACACGA ACATCGGCGA TATGATGCAA 
TTGATGGCAG CTGCTGAATT GCATAGTCGC GAGTGGCGAT ACCACGTGGA GGAGAAAATA 
TGGATAACTC GAATTCCTGG CATTGATCAA TATGAAAAAA ATGGTACAAA AGAGCGCGGC 
ACCTTCTACT ACTTTGATGC GCAAAGTTGG AAACGTTTGT CCAAGGTTTT CCAAATAGAT 
CCCGAGAAAT TAGATAAATG TCCCAATATA AGTGCGTTTA TGAATGGACA GTCTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rga ( NM_169080.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730966.1 Drosophila melanogaster regena, transcript variant A (Rga), mRNA.

Price & Availability↑

CloneID	OFa03966
Clone ID Related Accession (Same CDS sequence)	NM_169080.3
Accession Version	NM_169080.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	regena, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169080.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAATT TAAATTTTCA ACAACCCCCG AGAAGTATAG CGAACGCGGC GTTAGCTGGC 
CGGACAACAG GCGGATTCGG TGGTAGCTCC CTGGCTGGCC ATGTGACGCC CACGTCGGGC 
ATGTTTCAAA CCGACTTCGC CAACTCCTAT CCAGGAACGG CGAACTACGG ACAGGCGCCG 
CAGCAGCAGC AACAACAGCA GCAGCAGCCC CAACTGTCGC CGAACCGGAA TGCCCAGCTG 
TCGGTCGGCG GACCCGCCAT CTCGAGCGGC AACCGCAACG CCAACCTCTT CGGCCAGCGT 
CAGTTTGTGG AGCGGCGAGC CATGCAAGGA TTGGGCAGTG GCCCCATGTC GAACATGGGC 
AACTTCATGC AGACGGGTCG CGGCGGCTAC GGAACGGGCG GAGGCGGTGG CGGTCCTTTG 
AATAACTTCC ACGTGTTCGG CGGCGGCGGT GGTAGCGACA CCTCCACGCC TGCCCTGCTC 
GATCCCACGG AGTTCCCCTC GCTGACGAAT GCGCGCGGCC AGAACGACCA GACACTGCCC 
CAATCGAATC CGCTCCAGCC GCCGGGCAGC AAACCCTATG GTAATTTCTT TACCTCTTTT 
GGCATGGTGA AGCAACCGAC GTCAGAGCAA TCGGAGTTCA CGATGTCTAA CGAGGACTTC 
CCTGCGTTAC CGGGCACCCA AAACTCGGAC GGCACAACGA ACGCGGTGGG GAGCGTCGCC 
GGAACAGGAG GCTCCGGAGG TGCGTCCACA GAGAACCACT TGGACGGCAC GGAAAAGCCG 
ATGAATTCGA TAGTGGTCAG CGGATCAGCG AGCGGCAGCA GCGGTTCCAA CGTCGGCGTT 
GTAGGCGGCA ATGGTCTCGG TGCCGTTGGC AGTGGTATAG GGGGCTTGGC AGTAGGCGGC 
GGCGGAGGAG CTGGTAGCTC AGGAGGCGGA GGAGTCGGCG GCAATGCAGC CAGCGGCGTT 
GTCGGCGGGT CCCATGTAGG ACTGGTGGGC AGTAACAGCG GCATTGGCGG CGTCAACAGC 
GTACCCAACA GCAATGCCAT GATGGGCGTA GGCGGCGGCC TGGGGAGTGG AAGCGGCTCG 
TCTGGGAGCG GAGCGGGCGG CGAGCATCTG AACGATAATT CCAGCAACGA TAAACTTGTA 
AAGAGCGGCG TGCAAACTTC GCCAGATGGG AAGGTCACAA ACATACCAGC GACCATGGTG 
AACAACCAGT TCGGCATGGT TGGCCTGCTG ACGTTCATTC GGGCTGCAGA AACGGATCCC 
AATCTGGTGA CGCTCTCCTT GGGTACGGAT CTCACGGGTT TAGGCCTGAA CCTGAACTCG 
CAGGAGAGCT TGCATACAAC CTTCGCTGGC CCCTTCGTAG CACAGCCCTG CAGAGCACAA 
GACGTTGAGT TCAACGTGCC ACCCGAATAT CTGATTAACT TTGCGATAAG AGACAAACTA 
ACAGCGCCGG TACTGAAAAA GCTGCAAGAA GACCTGCTCT TTTTCCTTTT TTACACGAAC 
ATCGGCGATA TGATGCAATT GATGGCAGCT GCTGAATTGC ATAGTCGCGA GTGGCGATAC 
CACGTGGAGG AGAAAATATG GATAACTCGA ATTCCTGGCA TTGATCAATA TGAAAAAAAT 
GGTACAAAAG AGCGCGGCAC CTTCTACTAC TTTGATGCGC AAAGTTGGAA ACGTTTGTCC 
AAGGTTTTCC AAATAGATCC CGAGAAATTA GATAAATGTC CCAATATAAG TGCGTTTATG 
AATGGACAGT CTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730966.1
CDS	1047..2804
Translation	MANLNFQQPPRSIANAALAGRTTGGFGGSSLAGHVTPTSGMFQTDFANSYPGTANYGQAPQQQQQQQQQPQLSPNRNAQLSVGGPAISSGNRNANLFGQRQFVERRAMQGLGSGPMSNMGNFMQTGRGGYGTGGGGGGPLNNFHVFGGGGGSDTSTPALLDPTEFPSLTNARGQNDQTLPQSNPLQPPGSKPYGNFFTSFGMVKQPTSEQSEFTMSNEDFPALPGTQNSDGTTNAVGSVAGTGGSGGASTENHLDGTEKPMNSIVVSGSASGSSGSNVGVVGGNGLGAVGSGIGGLAVGGGGGAGSSGGGGVGGNAASGVVGGSHVGLVGSNSGIGGVNSVPNSNAMMGVGGGLGSGSGSSGSGAGGEHLNDNSSNDKLVKSGVQTSPDGKVTNIPATMVNNQFGMVGLLTFIRAAETDPNLVTLSLGTDLTGLGLNLNSQESLHTTFAGPFVAQPCRAQDVEFNVPPEYLINFAIRDKLTAPVLKKLQEDLLFFLFYTNIGDMMQLMAAAELHSREWRYHVEEKIWITRIPGIDQYEKNGTKERGTFYYFDAQSWKRLSKVFQIDPEKLDKCPNISAFMNGQSV

Target ORF information:

RefSeq Version	NM_169080.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster regena, transcript variant A (Rga), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169080.3

ORF Insert Sequence:

ATGGCGAATT TAAATTTTCA ACAACCCCCG AGAAGTATAG CGAACGCGGC GTTAGCTGGC 
CGGACAACAG GCGGATTCGG TGGTAGCTCC CTGGCTGGCC ATGTGACGCC CACGTCGGGC 
ATGTTTCAAA CCGACTTCGC CAACTCCTAT CCAGGAACGG CGAACTACGG ACAGGCGCCG 
CAGCAGCAGC AACAACAGCA GCAGCAGCCC CAACTGTCGC CGAACCGGAA TGCCCAGCTG 
TCGGTCGGCG GACCCGCCAT CTCGAGCGGC AACCGCAACG CCAACCTCTT CGGCCAGCGT 
CAGTTTGTGG AGCGGCGAGC CATGCAAGGA TTGGGCAGTG GCCCCATGTC GAACATGGGC 
AACTTCATGC AGACGGGTCG CGGCGGCTAC GGAACGGGCG GAGGCGGTGG CGGTCCTTTG 
AATAACTTCC ACGTGTTCGG CGGCGGCGGT GGTAGCGACA CCTCCACGCC TGCCCTGCTC 
GATCCCACGG AGTTCCCCTC GCTGACGAAT GCGCGCGGCC AGAACGACCA GACACTGCCC 
CAATCGAATC CGCTCCAGCC GCCGGGCAGC AAACCCTATG GTAATTTCTT TACCTCTTTT 
GGCATGGTGA AGCAACCGAC GTCAGAGCAA TCGGAGTTCA CGATGTCTAA CGAGGACTTC 
CCTGCGTTAC CGGGCACCCA AAACTCGGAC GGCACAACGA ACGCGGTGGG GAGCGTCGCC 
GGAACAGGAG GCTCCGGAGG TGCGTCCACA GAGAACCACT TGGACGGCAC GGAAAAGCCG 
ATGAATTCGA TAGTGGTCAG CGGATCAGCG AGCGGCAGCA GCGGTTCCAA CGTCGGCGTT 
GTAGGCGGCA ATGGTCTCGG TGCCGTTGGC AGTGGTATAG GGGGCTTGGC AGTAGGCGGC 
GGCGGAGGAG CTGGTAGCTC AGGAGGCGGA GGAGTCGGCG GCAATGCAGC CAGCGGCGTT 
GTCGGCGGGT CCCATGTAGG ACTGGTGGGC AGTAACAGCG GCATTGGCGG CGTCAACAGC 
GTACCCAACA GCAATGCCAT GATGGGCGTA GGCGGCGGCC TGGGGAGTGG AAGCGGCTCG 
TCTGGGAGCG GAGCGGGCGG CGAGCATCTG AACGATAATT CCAGCAACGA TAAACTTGTA 
AAGAGCGGCG TGCAAACTTC GCCAGATGGG AAGGTCACAA ACATACCAGC GACCATGGTG 
AACAACCAGT TCGGCATGGT TGGCCTGCTG ACGTTCATTC GGGCTGCAGA AACGGATCCC 
AATCTGGTGA CGCTCTCCTT GGGTACGGAT CTCACGGGTT TAGGCCTGAA CCTGAACTCG 
CAGGAGAGCT TGCATACAAC CTTCGCTGGC CCCTTCGTAG CACAGCCCTG CAGAGCACAA 
GACGTTGAGT TCAACGTGCC ACCCGAATAT CTGATTAACT TTGCGATAAG AGACAAACTA 
ACAGCGCCGG TACTGAAAAA GCTGCAAGAA GACCTGCTCT TTTTCCTTTT TTACACGAAC 
ATCGGCGATA TGATGCAATT GATGGCAGCT GCTGAATTGC ATAGTCGCGA GTGGCGATAC 
CACGTGGAGG AGAAAATATG GATAACTCGA ATTCCTGGCA TTGATCAATA TGAAAAAAAT 
GGTACAAAAG AGCGCGGCAC CTTCTACTAC TTTGATGCGC AAAGTTGGAA ACGTTTGTCC 
AAGGTTTTCC AAATAGATCC CGAGAAATTA GATAAATGTC CCAATATAAG TGCGTTTATG 
AATGGACAGT CTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rga cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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