ko cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ko
Entrez Gene ID	40330
Full Name	knockout
Synonyms	CG10573,Dmel\CG10573
General protein information	Preferred Names knockout Names CG10573 gene product from transcript CG10573-RA CG10573-PA CG10573-PB ko-PA ko-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78B1-78B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_079469.3	NP_524193.2	knockout, isoform A
NM_001275206.1	NP_001262135.1	knockout, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ko gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ko cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19179	NM_079469.3 Latest version!	Drosophila melanogaster knockout (ko), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa19179	NM_001275206.1 Latest version!	Drosophila melanogaster knockout (ko), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ko ( NM_079469.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524193.2 Drosophila melanogaster knockout (ko), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa19179
Clone ID Related Accession (Same CDS sequence)	NM_079469.3 , NM_001275206.1
Accession Version	NM_079469.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	knockout, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079469.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCACCA AGTCACCCGG TGGTCCACAG CGCTGCCGTC TGATCTTGCA GCGCTGCCTG 
GCCATTCAGC TGACCAAGCC CGGCCACACG CCCGAGGACT TTTGGATGTA CGACTCGGGA 
TATATGATAT TTCAGAACTT TCTAGCTGCC AATGCGCAAT GCTGGTGGAA TGGACCTTTG 
ACAGCTGCCA CAAGAGCGCT GAAATACGCA GGACATGTGG CGCCTGGAAT GCTGCTCGTT 
ACGGCTGAGC CCTGTGCCCT GGAGGTCCTG CGAGGAGCCT TTGCCCGAAG TGTCCTGAAG 
CCACCAGCCA CCTATGTGAT CAGCTCAGTG GGTGACATTG ACGATTGCAT TGTGACGCCA 
ACGGTTCAGG GACAGTTCAC GCCGCTTCCA GAGGCACTCT GCGATGTGAT CATGGACCTC 
ACCGCCGAGG GCCAGTCGGC AACCATTGAG CATGTGCGCA GCAAGCTGGG CTCCCGATTC 
CCACACATGA CCACGCCGGC CACCGAGGTC ATTTACGACT CCCTCGCCCA GCTGATGCAG 
GAGCAGAAGA TTTATCAGAC CTCCAAAGGA TACTTCATCT TCACACCAGA ACGCCGACGT 
AGTCGATCGA GGCCGCGCAG CAATCACCAC CAGTTGAATG GTAGTCTAGC CTATGGTCAC 
TTGGCGGAGG AGGAGCAGCA GCACGGTGAT GATGTGGTTC CCCAGGAGGC ACGCACAATG 
CTAATGTCCA ATTCCGAGGC CTTGCACTCT CTCTACGGAG AAATCTCAAC GGAGCGGGAC 
GGCGACCTCA CCCATCAGTG CATCCAAACG AATCTGGCGG ATGTGATCTG TGGCGGCAAT 
CCCAATGACA AGATCATCTA TCCAAGGGCG GCGAAGCGAC GCAGTGCATC GTTTCCGACG 
CCTCGCAGCT TGGAACGCCG GCACAGTTTG CGGCTCTTTG GCTCGTCGAA GCGCCTGCAA 
CGCTGCGCCT CCACGCGAAG TCTGTCCAAG ACGTACGCCC AAACGCTCAA CACCACGGAC 
AGCAGCAGCT CGGAATACCA AAGCACAGAT TCGTCATCAC CCAAAAAAGG ATCTTTGCTC 
TCACGACTGT TCCGACGCAG CGGTCGAGCC AAGCAGCGGC AGATTGAGAC CTATTCGGCC 
CAGTTCCCTC CAGCCGAGTG GTTCAACACC AGAGCCGTTC ACCTGCACAG CGTCGGCACC 
CAAACGGCCG AGCACGACAT GCCCGTTGCC CACACGCTGT CCAACTCGAC GTTCTACGAC 
GGCTCTGAGC TGAGCCAGCG CTCCTCGACG TTGCCCCGTC GCCATCGACG CCACATGTCC 
AGCGAATCGA CGTTTCTGAT TTCCTCCAGG GACTGCTCGC CCATCCGCCG CCGATCGCCG 
GTGTACTGCA GCAGTTCGCT ACCTCGTTCC ACGCAGTCGA TTCCGGCCAC CACGGCCAAG 
ACAAATCACT CGTCGCGTTT GAGTCATGGT CACGGAGGAT CTGTGACGCC AACGCCGCCA 
AAGACGGCAC AGAAGTCGGT GATTGGGAAG CACATCTTCG AGAGCTCGCC ATCGCGATCC 
GCCACGTTAA AAGCGACGGC GGCCAAGCGC CACACGGACG GACTGATCAT CAACAGCTGC 
CTGGACAACA AGGCCACGTA CCGGAGTCTG CAAAGCAGTG GTCCCTCGAG CCTCGAGTCC 
TGCAAGACTA CCTCGATGCT CGCCAGCGGT CCATCGAGTA TTGACAGCGG CAAGGTGTCC 
TTTAGCCAAA AGACCAGCGG CCACTCTAGT CTGGACTCGC AGTGCTCCAC GAATACGGCG 
GTGATAGCAC CACCATCGAA CAGTAACAAT GGGAGTTCTC GTTCGATTCT CCTGCTAAAC 
GGTTCTCCAC GAGGAACACC ACGTCATCAG GCCATGAGGG CACGAGTGGC CGAAGCACAG 
GCGCAAAGAC AAAGAGCTAG TACGCCCAGT CGCATCATGG AGGAAATCTA TCCGGCGGGT 
GGAAGCAATG GTGTTCCCAC CTCGAACAGC AATAACTCCA TTACCCTACA GGTTACCACC 
TCGAATGGGA ATCAAAGCAT ACCGAGCACC AAGATCTTTG TCCAAAACTC ACCGGTAAGG 
AGTGTTATAA CCCTGGAGAA CGGCAAGATG CTGGAGAACT CCAATGTATT CATCATCAAC 
AATGAGACGA TGACTAACGA GAAGGGAGAG ATCATCAAGA AAACCCTTTC CAAGCAGACT 
TCCGCTGCCC CAGCGGCCAC ATCTACGCCT GTTAAGCCTG AAACTGTTTC TGAGCAGCCA 
CTTAGTGAAA CGGAGGCCAA CTCGGAGACC TGCGACTCCA TTTCCTTCAT CAGTGAGTGT 
TCCCCGGAGA GCACGTGTGC TGTTGAACCG GAGACCCCTG TTTATGATGG AGGAAAGTAC 
GCCAAGAACA CCAATAACGA TGCCACCATG AACAACAGTC GGAAACTGTG CCTCCAACTG 
GCCAATGGCA TTGCGTATAA GAACAACGTG CTGAAGAATG AATCCCAACC AAATTCGCCC 
AGTGCGGATG CTCCTCTTAA AATGGCAGCA CTAGCCAAGC AGGATTTTGA GATTGCCGGC 
TCGGTGGGCA ACCTGAATTT TTACGAAAAG AGTTCGAAGG ATGTGGGTGC CTCACAGAGC 
AACAACATGA TGATGAACAG TAGTACCGAC CTCAAGAATC TGTGCTATGA GTCCGTGTGT 
CAGCTGCAGA AGTGCTCTAT GAATGGCGAT GAACTGGCGC TGGCCCATTT GCTACACAAG 
TCAAGCAATC CACTGGGTAG TGAGCCCAAT TTGGCTCTGA AAGATCTGGC GAATGATAAG 
TCCATTGATA AGGAAGCCAT CGACCGACGA CTCAGTTTGA CCAAGGAATC TTTGGAGCAA 
GGAATGGGCA GCGTGCTAGC CAACGGCGGC GAAGAGCTAT ATAACTTTCC CAGTCTGACC 
GACTTGTCCT TTAACTTTAC GTCCTTGGCG GCGAGAAAGA TCCTTCAGGG TGTGAGCCTC 
AACAGCATCG ACACCCTGGT GGAACTCAAC ATGGCAGCAG CGGCGGCGGC GGCAGCGGTG 
GCAAATAACG CGCAGGAGAA GCAGCAGAAC AACCAGCCAG CGACGGCAGG ACCAACTGCA 
TCCGTGTGCA CCGATTTTGG AATGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524193.2
CDS	954..4103
Translation	MGTKSPGGPQRCRLILQRCLAIQLTKPGHTPEDFWMYDSGYMIFQNFLAANAQCWWNGPLTAATRALKYAGHVAPGMLLVTAEPCALEVLRGAFARSVLKPPATYVISSVGDIDDCIVTPTVQGQFTPLPEALCDVIMDLTAEGQSATIEHVRSKLGSRFPHMTTPATEVIYDSLAQLMQEQKIYQTSKGYFIFTPERRRSRSRPRSNHHQLNGSLAYGHLAEEEQQHGDDVVPQEARTMLMSNSEALHSLYGEISTERDGDLTHQCIQTNLADVICGGNPNDKIIYPRAAKRRSASFPTPRSLERRHSLRLFGSSKRLQRCASTRSLSKTYAQTLNTTDSSSSEYQSTDSSSPKKGSLLSRLFRRSGRAKQRQIETYSAQFPPAEWFNTRAVHLHSVGTQTAEHDMPVAHTLSNSTFYDGSELSQRSSTLPRRHRRHMSSESTFLISSRDCSPIRRRSPVYCSSSLPRSTQSIPATTAKTNHSSRLSHGHGGSVTPTPPKTAQKSVIGKHIFESSPSRSATLKATAAKRHTDGLIINSCLDNKATYRSLQSSGPSSLESCKTTSMLASGPSSIDSGKVSFSQKTSGHSSLDSQCSTNTAVIAPPSNSNNGSSRSILLLNGSPRGTPRHQAMRARVAEAQAQRQRASTPSRIMEEIYPAGGSNGVPTSNSNNSITLQVTTSNGNQSIPSTKIFVQNSPVRSVITLENGKMLENSNVFIINNETMTNEKGEIIKKTLSKQTSAAPAATSTPVKPETVSEQPLSETEANSETCDSISFISECSPESTCAVEPETPVYDGGKYAKNTNNDATMNNSRKLCLQLANGIAYKNNVLKNESQPNSPSADAPLKMAALAKQDFEIAGSVGNLNFYEKSSKDVGASQSNNMMMNSSTDLKNLCYESVCQLQKCSMNGDELALAHLLHKSSNPLGSEPNLALKDLANDKSIDKEAIDRRLSLTKESLEQGMGSVLANGGEELYNFPSLTDLSFNFTSLAARKILQGVSLNSIDTLVELNMAAAAAAAAVANNAQEKQQNNQPATAGPTASVCTDFGMV

Target ORF information:

RefSeq Version	NM_079469.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster knockout (ko), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079469.3

ORF Insert Sequence:

ATGGGCACCA AGTCACCCGG TGGTCCACAG CGCTGCCGTC TGATCTTGCA GCGCTGCCTG 
GCCATTCAGC TGACCAAGCC CGGCCACACG CCCGAGGACT TTTGGATGTA CGACTCGGGA 
TATATGATAT TTCAGAACTT TCTAGCTGCC AATGCGCAAT GCTGGTGGAA TGGACCTTTG 
ACAGCTGCCA CAAGAGCGCT GAAATACGCA GGACATGTGG CGCCTGGAAT GCTGCTCGTT 
ACGGCTGAGC CCTGTGCCCT GGAGGTCCTG CGAGGAGCCT TTGCCCGAAG TGTCCTGAAG 
CCACCAGCCA CCTATGTGAT CAGCTCAGTG GGTGACATTG ACGATTGCAT TGTGACGCCA 
ACGGTTCAGG GACAGTTCAC GCCGCTTCCA GAGGCACTCT GCGATGTGAT CATGGACCTC 
ACCGCCGAGG GCCAGTCGGC AACCATTGAG CATGTGCGCA GCAAGCTGGG CTCCCGATTC 
CCACACATGA CCACGCCGGC CACCGAGGTC ATTTACGACT CCCTCGCCCA GCTGATGCAG 
GAGCAGAAGA TTTATCAGAC CTCCAAAGGA TACTTCATCT TCACACCAGA ACGCCGACGT 
AGTCGATCGA GGCCGCGCAG CAATCACCAC CAGTTGAATG GTAGTCTAGC CTATGGTCAC 
TTGGCGGAGG AGGAGCAGCA GCACGGTGAT GATGTGGTTC CCCAGGAGGC ACGCACAATG 
CTAATGTCCA ATTCCGAGGC CTTGCACTCT CTCTACGGAG AAATCTCAAC GGAGCGGGAC 
GGCGACCTCA CCCATCAGTG CATCCAAACG AATCTGGCGG ATGTGATCTG TGGCGGCAAT 
CCCAATGACA AGATCATCTA TCCAAGGGCG GCGAAGCGAC GCAGTGCATC GTTTCCGACG 
CCTCGCAGCT TGGAACGCCG GCACAGTTTG CGGCTCTTTG GCTCGTCGAA GCGCCTGCAA 
CGCTGCGCCT CCACGCGAAG TCTGTCCAAG ACGTACGCCC AAACGCTCAA CACCACGGAC 
AGCAGCAGCT CGGAATACCA AAGCACAGAT TCGTCATCAC CCAAAAAAGG ATCTTTGCTC 
TCACGACTGT TCCGACGCAG CGGTCGAGCC AAGCAGCGGC AGATTGAGAC CTATTCGGCC 
CAGTTCCCTC CAGCCGAGTG GTTCAACACC AGAGCCGTTC ACCTGCACAG CGTCGGCACC 
CAAACGGCCG AGCACGACAT GCCCGTTGCC CACACGCTGT CCAACTCGAC GTTCTACGAC 
GGCTCTGAGC TGAGCCAGCG CTCCTCGACG TTGCCCCGTC GCCATCGACG CCACATGTCC 
AGCGAATCGA CGTTTCTGAT TTCCTCCAGG GACTGCTCGC CCATCCGCCG CCGATCGCCG 
GTGTACTGCA GCAGTTCGCT ACCTCGTTCC ACGCAGTCGA TTCCGGCCAC CACGGCCAAG 
ACAAATCACT CGTCGCGTTT GAGTCATGGT CACGGAGGAT CTGTGACGCC AACGCCGCCA 
AAGACGGCAC AGAAGTCGGT GATTGGGAAG CACATCTTCG AGAGCTCGCC ATCGCGATCC 
GCCACGTTAA AAGCGACGGC GGCCAAGCGC CACACGGACG GACTGATCAT CAACAGCTGC 
CTGGACAACA AGGCCACGTA CCGGAGTCTG CAAAGCAGTG GTCCCTCGAG CCTCGAGTCC 
TGCAAGACTA CCTCGATGCT CGCCAGCGGT CCATCGAGTA TTGACAGCGG CAAGGTGTCC 
TTTAGCCAAA AGACCAGCGG CCACTCTAGT CTGGACTCGC AGTGCTCCAC GAATACGGCG 
GTGATAGCAC CACCATCGAA CAGTAACAAT GGGAGTTCTC GTTCGATTCT CCTGCTAAAC 
GGTTCTCCAC GAGGAACACC ACGTCATCAG GCCATGAGGG CACGAGTGGC CGAAGCACAG 
GCGCAAAGAC AAAGAGCTAG TACGCCCAGT CGCATCATGG AGGAAATCTA TCCGGCGGGT 
GGAAGCAATG GTGTTCCCAC CTCGAACAGC AATAACTCCA TTACCCTACA GGTTACCACC 
TCGAATGGGA ATCAAAGCAT ACCGAGCACC AAGATCTTTG TCCAAAACTC ACCGGTAAGG 
AGTGTTATAA CCCTGGAGAA CGGCAAGATG CTGGAGAACT CCAATGTATT CATCATCAAC 
AATGAGACGA TGACTAACGA GAAGGGAGAG ATCATCAAGA AAACCCTTTC CAAGCAGACT 
TCCGCTGCCC CAGCGGCCAC ATCTACGCCT GTTAAGCCTG AAACTGTTTC TGAGCAGCCA 
CTTAGTGAAA CGGAGGCCAA CTCGGAGACC TGCGACTCCA TTTCCTTCAT CAGTGAGTGT 
TCCCCGGAGA GCACGTGTGC TGTTGAACCG GAGACCCCTG TTTATGATGG AGGAAAGTAC 
GCCAAGAACA CCAATAACGA TGCCACCATG AACAACAGTC GGAAACTGTG CCTCCAACTG 
GCCAATGGCA TTGCGTATAA GAACAACGTG CTGAAGAATG AATCCCAACC AAATTCGCCC 
AGTGCGGATG CTCCTCTTAA AATGGCAGCA CTAGCCAAGC AGGATTTTGA GATTGCCGGC 
TCGGTGGGCA ACCTGAATTT TTACGAAAAG AGTTCGAAGG ATGTGGGTGC CTCACAGAGC 
AACAACATGA TGATGAACAG TAGTACCGAC CTCAAGAATC TGTGCTATGA GTCCGTGTGT 
CAGCTGCAGA AGTGCTCTAT GAATGGCGAT GAACTGGCGC TGGCCCATTT GCTACACAAG 
TCAAGCAATC CACTGGGTAG TGAGCCCAAT TTGGCTCTGA AAGATCTGGC GAATGATAAG 
TCCATTGATA AGGAAGCCAT CGACCGACGA CTCAGTTTGA CCAAGGAATC TTTGGAGCAA 
GGAATGGGCA GCGTGCTAGC CAACGGCGGC GAAGAGCTAT ATAACTTTCC CAGTCTGACC 
GACTTGTCCT TTAACTTTAC GTCCTTGGCG GCGAGAAAGA TCCTTCAGGG TGTGAGCCTC 
AACAGCATCG ACACCCTGGT GGAACTCAAC ATGGCAGCAG CGGCGGCGGC GGCAGCGGTG 
GCAAATAACG CGCAGGAGAA GCAGCAGAAC AACCAGCCAG CGACGGCAGG ACCAACTGCA 
TCCGTGTGCA CCGATTTTGG AATGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ko ( NM_001275206.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262135.1 Drosophila melanogaster knockout (ko), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa19179
Clone ID Related Accession (Same CDS sequence)	NM_079469.3 , NM_001275206.1
Accession Version	NM_001275206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	knockout, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCACCA AGTCACCCGG TGGTCCACAG CGCTGCCGTC TGATCTTGCA GCGCTGCCTG 
GCCATTCAGC TGACCAAGCC CGGCCACACG CCCGAGGACT TTTGGATGTA CGACTCGGGA 
TATATGATAT TTCAGAACTT TCTAGCTGCC AATGCGCAAT GCTGGTGGAA TGGACCTTTG 
ACAGCTGCCA CAAGAGCGCT GAAATACGCA GGACATGTGG CGCCTGGAAT GCTGCTCGTT 
ACGGCTGAGC CCTGTGCCCT GGAGGTCCTG CGAGGAGCCT TTGCCCGAAG TGTCCTGAAG 
CCACCAGCCA CCTATGTGAT CAGCTCAGTG GGTGACATTG ACGATTGCAT TGTGACGCCA 
ACGGTTCAGG GACAGTTCAC GCCGCTTCCA GAGGCACTCT GCGATGTGAT CATGGACCTC 
ACCGCCGAGG GCCAGTCGGC AACCATTGAG CATGTGCGCA GCAAGCTGGG CTCCCGATTC 
CCACACATGA CCACGCCGGC CACCGAGGTC ATTTACGACT CCCTCGCCCA GCTGATGCAG 
GAGCAGAAGA TTTATCAGAC CTCCAAAGGA TACTTCATCT TCACACCAGA ACGCCGACGT 
AGTCGATCGA GGCCGCGCAG CAATCACCAC CAGTTGAATG GTAGTCTAGC CTATGGTCAC 
TTGGCGGAGG AGGAGCAGCA GCACGGTGAT GATGTGGTTC CCCAGGAGGC ACGCACAATG 
CTAATGTCCA ATTCCGAGGC CTTGCACTCT CTCTACGGAG AAATCTCAAC GGAGCGGGAC 
GGCGACCTCA CCCATCAGTG CATCCAAACG AATCTGGCGG ATGTGATCTG TGGCGGCAAT 
CCCAATGACA AGATCATCTA TCCAAGGGCG GCGAAGCGAC GCAGTGCATC GTTTCCGACG 
CCTCGCAGCT TGGAACGCCG GCACAGTTTG CGGCTCTTTG GCTCGTCGAA GCGCCTGCAA 
CGCTGCGCCT CCACGCGAAG TCTGTCCAAG ACGTACGCCC AAACGCTCAA CACCACGGAC 
AGCAGCAGCT CGGAATACCA AAGCACAGAT TCGTCATCAC CCAAAAAAGG ATCTTTGCTC 
TCACGACTGT TCCGACGCAG CGGTCGAGCC AAGCAGCGGC AGATTGAGAC CTATTCGGCC 
CAGTTCCCTC CAGCCGAGTG GTTCAACACC AGAGCCGTTC ACCTGCACAG CGTCGGCACC 
CAAACGGCCG AGCACGACAT GCCCGTTGCC CACACGCTGT CCAACTCGAC GTTCTACGAC 
GGCTCTGAGC TGAGCCAGCG CTCCTCGACG TTGCCCCGTC GCCATCGACG CCACATGTCC 
AGCGAATCGA CGTTTCTGAT TTCCTCCAGG GACTGCTCGC CCATCCGCCG CCGATCGCCG 
GTGTACTGCA GCAGTTCGCT ACCTCGTTCC ACGCAGTCGA TTCCGGCCAC CACGGCCAAG 
ACAAATCACT CGTCGCGTTT GAGTCATGGT CACGGAGGAT CTGTGACGCC AACGCCGCCA 
AAGACGGCAC AGAAGTCGGT GATTGGGAAG CACATCTTCG AGAGCTCGCC ATCGCGATCC 
GCCACGTTAA AAGCGACGGC GGCCAAGCGC CACACGGACG GACTGATCAT CAACAGCTGC 
CTGGACAACA AGGCCACGTA CCGGAGTCTG CAAAGCAGTG GTCCCTCGAG CCTCGAGTCC 
TGCAAGACTA CCTCGATGCT CGCCAGCGGT CCATCGAGTA TTGACAGCGG CAAGGTGTCC 
TTTAGCCAAA AGACCAGCGG CCACTCTAGT CTGGACTCGC AGTGCTCCAC GAATACGGCG 
GTGATAGCAC CACCATCGAA CAGTAACAAT GGGAGTTCTC GTTCGATTCT CCTGCTAAAC 
GGTTCTCCAC GAGGAACACC ACGTCATCAG GCCATGAGGG CACGAGTGGC CGAAGCACAG 
GCGCAAAGAC AAAGAGCTAG TACGCCCAGT CGCATCATGG AGGAAATCTA TCCGGCGGGT 
GGAAGCAATG GTGTTCCCAC CTCGAACAGC AATAACTCCA TTACCCTACA GGTTACCACC 
TCGAATGGGA ATCAAAGCAT ACCGAGCACC AAGATCTTTG TCCAAAACTC ACCGGTAAGG 
AGTGTTATAA CCCTGGAGAA CGGCAAGATG CTGGAGAACT CCAATGTATT CATCATCAAC 
AATGAGACGA TGACTAACGA GAAGGGAGAG ATCATCAAGA AAACCCTTTC CAAGCAGACT 
TCCGCTGCCC CAGCGGCCAC ATCTACGCCT GTTAAGCCTG AAACTGTTTC TGAGCAGCCA 
CTTAGTGAAA CGGAGGCCAA CTCGGAGACC TGCGACTCCA TTTCCTTCAT CAGTGAGTGT 
TCCCCGGAGA GCACGTGTGC TGTTGAACCG GAGACCCCTG TTTATGATGG AGGAAAGTAC 
GCCAAGAACA CCAATAACGA TGCCACCATG AACAACAGTC GGAAACTGTG CCTCCAACTG 
GCCAATGGCA TTGCGTATAA GAACAACGTG CTGAAGAATG AATCCCAACC AAATTCGCCC 
AGTGCGGATG CTCCTCTTAA AATGGCAGCA CTAGCCAAGC AGGATTTTGA GATTGCCGGC 
TCGGTGGGCA ACCTGAATTT TTACGAAAAG AGTTCGAAGG ATGTGGGTGC CTCACAGAGC 
AACAACATGA TGATGAACAG TAGTACCGAC CTCAAGAATC TGTGCTATGA GTCCGTGTGT 
CAGCTGCAGA AGTGCTCTAT GAATGGCGAT GAACTGGCGC TGGCCCATTT GCTACACAAG 
TCAAGCAATC CACTGGGTAG TGAGCCCAAT TTGGCTCTGA AAGATCTGGC GAATGATAAG 
TCCATTGATA AGGAAGCCAT CGACCGACGA CTCAGTTTGA CCAAGGAATC TTTGGAGCAA 
GGAATGGGCA GCGTGCTAGC CAACGGCGGC GAAGAGCTAT ATAACTTTCC CAGTCTGACC 
GACTTGTCCT TTAACTTTAC GTCCTTGGCG GCGAGAAAGA TCCTTCAGGG TGTGAGCCTC 
AACAGCATCG ACACCCTGGT GGAACTCAAC ATGGCAGCAG CGGCGGCGGC GGCAGCGGTG 
GCAAATAACG CGCAGGAGAA GCAGCAGAAC AACCAGCCAG CGACGGCAGG ACCAACTGCA 
TCCGTGTGCA CCGATTTTGG AATGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262135.1
CDS	506..3655
Translation	MGTKSPGGPQRCRLILQRCLAIQLTKPGHTPEDFWMYDSGYMIFQNFLAANAQCWWNGPLTAATRALKYAGHVAPGMLLVTAEPCALEVLRGAFARSVLKPPATYVISSVGDIDDCIVTPTVQGQFTPLPEALCDVIMDLTAEGQSATIEHVRSKLGSRFPHMTTPATEVIYDSLAQLMQEQKIYQTSKGYFIFTPERRRSRSRPRSNHHQLNGSLAYGHLAEEEQQHGDDVVPQEARTMLMSNSEALHSLYGEISTERDGDLTHQCIQTNLADVICGGNPNDKIIYPRAAKRRSASFPTPRSLERRHSLRLFGSSKRLQRCASTRSLSKTYAQTLNTTDSSSSEYQSTDSSSPKKGSLLSRLFRRSGRAKQRQIETYSAQFPPAEWFNTRAVHLHSVGTQTAEHDMPVAHTLSNSTFYDGSELSQRSSTLPRRHRRHMSSESTFLISSRDCSPIRRRSPVYCSSSLPRSTQSIPATTAKTNHSSRLSHGHGGSVTPTPPKTAQKSVIGKHIFESSPSRSATLKATAAKRHTDGLIINSCLDNKATYRSLQSSGPSSLESCKTTSMLASGPSSIDSGKVSFSQKTSGHSSLDSQCSTNTAVIAPPSNSNNGSSRSILLLNGSPRGTPRHQAMRARVAEAQAQRQRASTPSRIMEEIYPAGGSNGVPTSNSNNSITLQVTTSNGNQSIPSTKIFVQNSPVRSVITLENGKMLENSNVFIINNETMTNEKGEIIKKTLSKQTSAAPAATSTPVKPETVSEQPLSETEANSETCDSISFISECSPESTCAVEPETPVYDGGKYAKNTNNDATMNNSRKLCLQLANGIAYKNNVLKNESQPNSPSADAPLKMAALAKQDFEIAGSVGNLNFYEKSSKDVGASQSNNMMMNSSTDLKNLCYESVCQLQKCSMNGDELALAHLLHKSSNPLGSEPNLALKDLANDKSIDKEAIDRRLSLTKESLEQGMGSVLANGGEELYNFPSLTDLSFNFTSLAARKILQGVSLNSIDTLVELNMAAAAAAAAVANNAQEKQQNNQPATAGPTASVCTDFGMV

Target ORF information:

RefSeq Version	NM_001275206.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster knockout (ko), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275206.1

ORF Insert Sequence:

ATGGGCACCA AGTCACCCGG TGGTCCACAG CGCTGCCGTC TGATCTTGCA GCGCTGCCTG 
GCCATTCAGC TGACCAAGCC CGGCCACACG CCCGAGGACT TTTGGATGTA CGACTCGGGA 
TATATGATAT TTCAGAACTT TCTAGCTGCC AATGCGCAAT GCTGGTGGAA TGGACCTTTG 
ACAGCTGCCA CAAGAGCGCT GAAATACGCA GGACATGTGG CGCCTGGAAT GCTGCTCGTT 
ACGGCTGAGC CCTGTGCCCT GGAGGTCCTG CGAGGAGCCT TTGCCCGAAG TGTCCTGAAG 
CCACCAGCCA CCTATGTGAT CAGCTCAGTG GGTGACATTG ACGATTGCAT TGTGACGCCA 
ACGGTTCAGG GACAGTTCAC GCCGCTTCCA GAGGCACTCT GCGATGTGAT CATGGACCTC 
ACCGCCGAGG GCCAGTCGGC AACCATTGAG CATGTGCGCA GCAAGCTGGG CTCCCGATTC 
CCACACATGA CCACGCCGGC CACCGAGGTC ATTTACGACT CCCTCGCCCA GCTGATGCAG 
GAGCAGAAGA TTTATCAGAC CTCCAAAGGA TACTTCATCT TCACACCAGA ACGCCGACGT 
AGTCGATCGA GGCCGCGCAG CAATCACCAC CAGTTGAATG GTAGTCTAGC CTATGGTCAC 
TTGGCGGAGG AGGAGCAGCA GCACGGTGAT GATGTGGTTC CCCAGGAGGC ACGCACAATG 
CTAATGTCCA ATTCCGAGGC CTTGCACTCT CTCTACGGAG AAATCTCAAC GGAGCGGGAC 
GGCGACCTCA CCCATCAGTG CATCCAAACG AATCTGGCGG ATGTGATCTG TGGCGGCAAT 
CCCAATGACA AGATCATCTA TCCAAGGGCG GCGAAGCGAC GCAGTGCATC GTTTCCGACG 
CCTCGCAGCT TGGAACGCCG GCACAGTTTG CGGCTCTTTG GCTCGTCGAA GCGCCTGCAA 
CGCTGCGCCT CCACGCGAAG TCTGTCCAAG ACGTACGCCC AAACGCTCAA CACCACGGAC 
AGCAGCAGCT CGGAATACCA AAGCACAGAT TCGTCATCAC CCAAAAAAGG ATCTTTGCTC 
TCACGACTGT TCCGACGCAG CGGTCGAGCC AAGCAGCGGC AGATTGAGAC CTATTCGGCC 
CAGTTCCCTC CAGCCGAGTG GTTCAACACC AGAGCCGTTC ACCTGCACAG CGTCGGCACC 
CAAACGGCCG AGCACGACAT GCCCGTTGCC CACACGCTGT CCAACTCGAC GTTCTACGAC 
GGCTCTGAGC TGAGCCAGCG CTCCTCGACG TTGCCCCGTC GCCATCGACG CCACATGTCC 
AGCGAATCGA CGTTTCTGAT TTCCTCCAGG GACTGCTCGC CCATCCGCCG CCGATCGCCG 
GTGTACTGCA GCAGTTCGCT ACCTCGTTCC ACGCAGTCGA TTCCGGCCAC CACGGCCAAG 
ACAAATCACT CGTCGCGTTT GAGTCATGGT CACGGAGGAT CTGTGACGCC AACGCCGCCA 
AAGACGGCAC AGAAGTCGGT GATTGGGAAG CACATCTTCG AGAGCTCGCC ATCGCGATCC 
GCCACGTTAA AAGCGACGGC GGCCAAGCGC CACACGGACG GACTGATCAT CAACAGCTGC 
CTGGACAACA AGGCCACGTA CCGGAGTCTG CAAAGCAGTG GTCCCTCGAG CCTCGAGTCC 
TGCAAGACTA CCTCGATGCT CGCCAGCGGT CCATCGAGTA TTGACAGCGG CAAGGTGTCC 
TTTAGCCAAA AGACCAGCGG CCACTCTAGT CTGGACTCGC AGTGCTCCAC GAATACGGCG 
GTGATAGCAC CACCATCGAA CAGTAACAAT GGGAGTTCTC GTTCGATTCT CCTGCTAAAC 
GGTTCTCCAC GAGGAACACC ACGTCATCAG GCCATGAGGG CACGAGTGGC CGAAGCACAG 
GCGCAAAGAC AAAGAGCTAG TACGCCCAGT CGCATCATGG AGGAAATCTA TCCGGCGGGT 
GGAAGCAATG GTGTTCCCAC CTCGAACAGC AATAACTCCA TTACCCTACA GGTTACCACC 
TCGAATGGGA ATCAAAGCAT ACCGAGCACC AAGATCTTTG TCCAAAACTC ACCGGTAAGG 
AGTGTTATAA CCCTGGAGAA CGGCAAGATG CTGGAGAACT CCAATGTATT CATCATCAAC 
AATGAGACGA TGACTAACGA GAAGGGAGAG ATCATCAAGA AAACCCTTTC CAAGCAGACT 
TCCGCTGCCC CAGCGGCCAC ATCTACGCCT GTTAAGCCTG AAACTGTTTC TGAGCAGCCA 
CTTAGTGAAA CGGAGGCCAA CTCGGAGACC TGCGACTCCA TTTCCTTCAT CAGTGAGTGT 
TCCCCGGAGA GCACGTGTGC TGTTGAACCG GAGACCCCTG TTTATGATGG AGGAAAGTAC 
GCCAAGAACA CCAATAACGA TGCCACCATG AACAACAGTC GGAAACTGTG CCTCCAACTG 
GCCAATGGCA TTGCGTATAA GAACAACGTG CTGAAGAATG AATCCCAACC AAATTCGCCC 
AGTGCGGATG CTCCTCTTAA AATGGCAGCA CTAGCCAAGC AGGATTTTGA GATTGCCGGC 
TCGGTGGGCA ACCTGAATTT TTACGAAAAG AGTTCGAAGG ATGTGGGTGC CTCACAGAGC 
AACAACATGA TGATGAACAG TAGTACCGAC CTCAAGAATC TGTGCTATGA GTCCGTGTGT 
CAGCTGCAGA AGTGCTCTAT GAATGGCGAT GAACTGGCGC TGGCCCATTT GCTACACAAG 
TCAAGCAATC CACTGGGTAG TGAGCCCAAT TTGGCTCTGA AAGATCTGGC GAATGATAAG 
TCCATTGATA AGGAAGCCAT CGACCGACGA CTCAGTTTGA CCAAGGAATC TTTGGAGCAA 
GGAATGGGCA GCGTGCTAGC CAACGGCGGC GAAGAGCTAT ATAACTTTCC CAGTCTGACC 
GACTTGTCCT TTAACTTTAC GTCCTTGGCG GCGAGAAAGA TCCTTCAGGG TGTGAGCCTC 
AACAGCATCG ACACCCTGGT GGAACTCAAC ATGGCAGCAG CGGCGGCGGC GGCAGCGGTG 
GCAAATAACG CGCAGGAGAA GCAGCAGAAC AACCAGCCAG CGACGGCAGG ACCAACTGCA 
TCCGTGTGCA CCGATTTTGG AATGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ko cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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