hid cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hid
Entrez Gene ID	40009
Full Name	head involution defective
Synonyms	CG5123,Dmel\CG5123,HID,Hid,Hid1,W,hid1,his,l(3)05014
General protein information	Preferred Names head involution defective Names CG5123 gene product from transcript CG5123-RA CG5123-PA CG5123-PB head involuted head involuted defective head involution defect head involution defective 1 head-involution defective head-involution-defective hid-PA hid-PB wrinkled wrinkler
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 75C1-75C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_079412.4	NP_524136.2	head involution defective, isoform A
NM_001275081.1	NP_001262010.1	head involution defective, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

hid is required during pupariation for caspase-dependent pruning of class IV da neurons and Nos and Pum delay pruning.
Title: Nanos-mediated repression of hid protects larval sensory neurons after a global switch in sensitivity to apoptotic signals.

loss of function mutants shows persisting wing epithelial cells and, furthermore, silencing of the hormone bursicon in the CNS produced collective cell death defective phenotypes manifested in the wing epithelium
Title: Hid arbitrates collective cell death in the Drosophila wing.

conclude that dLin52 functions as a co-factor and modulates activity of members of dMyb/dREAM complex at hid promoter, thus regulating apoptosis by repressing this pro-apoptotic gene in the developing Drosophila eye
Title: dLin52 is crucial for dE2F and dRBF mediated transcriptional regulation of pro-apoptotic gene hid.

Shows that in addition to specifying distinct neuronal subtypes over time, temporal patterning of outer proliferation center progenitors also controls the transition in apoptosis by specifying the systematic death of Notch ON neurons in a first phase and of Notch OFF neurons in a second phase through the regulation of Rpr and Hid, respectively.
Title: Temporal patterning of neuroblasts controls Notch-mediated cell survival through regulation of Hid or Reaper.

Study provide evidence that miR-6/11 functions through regulation of the proapoptotic genes, reaper (rpr), head involution defective (hid), grim and sickle (skl).
Title: Overlapping functions of microRNAs in control of apoptosis during Drosophila embryogenesis.

The following hid gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hid cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18852	NM_079412.4 Latest version!	Drosophila melanogaster head involution defective (hid), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18852	NM_001275081.1 Latest version!	Drosophila melanogaster head involution defective (hid), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hid ( NM_079412.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524136.2 Drosophila melanogaster head involution defective (hid), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18852
Clone ID Related Accession (Same CDS sequence)	NM_079412.4 , NM_001275081.1
Accession Version	NM_079412.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	head involution defective, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_079412.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGTGC CCTTTTATTT GCCCGAGGGC GGCGCCGATG ACGTAGCGTC GAGTTCATCG 
GGAGCCTCGG GCAACTCCTC CCCCCACAAC CACCCACTTC CCTCGAGCGC ATCCTCGTCC 
GTCTCCTCCT CGGGCGTGTC CTCGGCCTCC GCCTCCTCGG CCTCATCTTC GTCCTCCGCA 
TCGTCGGACG GCGCCAGCAG CGCCGCCTCG CAATCGCCGA ACACCACCAC CTCGTCGGCC 
ACGCAGACGC CGATGCAGTC TCCACTGCCC ACCGACCAAG TGCTATACGC CCTCTACGAG 
TGGGTCAGGA TGTACCAGAG CCAGCAGAGT GCCCCGCAAA TCTTCCAGTA TCCGCCGCCA 
AGCCCCTCTT GCAATTTCAC GGGCGGCGAT GTGTTCTTTC CGCACGGCCA TCCGAATCCG 
AACTCGAATC CCCATCCGCG CACCCCCCGA ACCAGCGTGA GCTTCTCCTC CGGCGAGGAG 
TACAACTTCT TCCGGCAGCA GCAGCCGCAA CCACATCCGT CATATCCGGC GCCATCAACA 
CCGCAGCCAA TGCCACCGCA GTCAGCGCCG CCGATGCACT GCAGCCACAG CTACCCGCAG 
CAGTCGGCGC ACATGATGCC ACACCATTCC GCTCCCTTCG GAATGGGCGG TACCTACTAC 
GCGGGCTACA CGCCGCCACC CACTCCGAAC ACGGCCAGTG CGGGCACCTC CAGCTCATCG 
GCGGCCTTCG GCTGGCACGG CCACCCCCAC AGCCCCTTCA CGTCGACCTC CACGCCGTTA 
TCGGCGCCAG TGGCGCCCAA GATGCGCCTG CAGCGCAGCC AGTCGGATGC GGCCAGACGC 
AAGCGATTGA CCTCGACGGG CGAGGATGAG CGCGAGTACC AGAGCGATCA TGAGGCCACT 
TGGGACGAGT TTGGCGATCG CTACGACAAC TTTACGGCCG GCCGGGAGCG TCTGCAGGAG 
TTCAATGGAC GCATCCCGCC CCGGAAGAAG AAGAGCTCCA ATAGCCACTC GAGCAGCAGC 
AATAATCCAG TCTGCCATAC CGATAGCCAG CCCGGTGGTA CATCCCAAGC GGAGAGCGGT 
GCCATCCATG GCCACATCAG TCAGCAGCGA CAGGTGGAGC GAGAACGACA AAAGGCGAAG 
GCCGAGAAGA AGAAACCACA GAGCTTCACT TGGCCAACTG TTGTGACCGT TTTCGTTTTG 
GCCATGGGCT GTGGCTTCTT TGCGGCGCGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524136.2
CDS	520..1752
Translation	MAVPFYLPEGGADDVASSSSGASGNSSPHNHPLPSSASSSVSSSGVSSASASSASSSSSASSDGASSAASQSPNTTTSSATQTPMQSPLPTDQVLYALYEWVRMYQSQQSAPQIFQYPPPSPSCNFTGGDVFFPHGHPNPNSNPHPRTPRTSVSFSSGEEYNFFRQQQPQPHPSYPAPSTPQPMPPQSAPPMHCSHSYPQQSAHMMPHHSAPFGMGGTYYAGYTPPPTPNTASAGTSSSSAAFGWHGHPHSPFTSTSTPLSAPVAPKMRLQRSQSDAARRKRLTSTGEDEREYQSDHEATWDEFGDRYDNFTAGRERLQEFNGRIPPRKKKSSNSHSSSSNNPVCHTDSQPGGTSQAESGAIHGHISQQRQVERERQKAKAEKKKPQSFTWPTVVTVFVLAMGCGFFAAR

Target ORF information:

RefSeq Version	NM_079412.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster head involution defective (hid), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079412.4

ORF Insert Sequence:

ATGGCCGTGC CCTTTTATTT GCCCGAGGGC GGCGCCGATG ACGTAGCGTC GAGTTCATCG 
GGAGCCTCGG GCAACTCCTC CCCCCACAAC CACCCACTTC CCTCGAGCGC ATCCTCGTCC 
GTCTCCTCCT CGGGCGTGTC CTCGGCCTCC GCCTCCTCGG CCTCATCTTC GTCCTCCGCA 
TCGTCGGACG GCGCCAGCAG CGCCGCCTCG CAATCGCCGA ACACCACCAC CTCGTCGGCC 
ACGCAGACGC CGATGCAGTC TCCACTGCCC ACCGACCAAG TGCTATACGC CCTCTACGAG 
TGGGTCAGGA TGTACCAGAG CCAGCAGAGT GCCCCGCAAA TCTTCCAGTA TCCGCCGCCA 
AGCCCCTCTT GCAATTTCAC GGGCGGCGAT GTGTTCTTTC CGCACGGCCA TCCGAATCCG 
AACTCGAATC CCCATCCGCG CACCCCCCGA ACCAGCGTGA GCTTCTCCTC CGGCGAGGAG 
TACAACTTCT TCCGGCAGCA GCAGCCGCAA CCACATCCGT CATATCCGGC GCCATCAACA 
CCGCAGCCAA TGCCACCGCA GTCAGCGCCG CCGATGCACT GCAGCCACAG CTACCCGCAG 
CAGTCGGCGC ACATGATGCC ACACCATTCC GCTCCCTTCG GAATGGGCGG TACCTACTAC 
GCGGGCTACA CGCCGCCACC CACTCCGAAC ACGGCCAGTG CGGGCACCTC CAGCTCATCG 
GCGGCCTTCG GCTGGCACGG CCACCCCCAC AGCCCCTTCA CGTCGACCTC CACGCCGTTA 
TCGGCGCCAG TGGCGCCCAA GATGCGCCTG CAGCGCAGCC AGTCGGATGC GGCCAGACGC 
AAGCGATTGA CCTCGACGGG CGAGGATGAG CGCGAGTACC AGAGCGATCA TGAGGCCACT 
TGGGACGAGT TTGGCGATCG CTACGACAAC TTTACGGCCG GCCGGGAGCG TCTGCAGGAG 
TTCAATGGAC GCATCCCGCC CCGGAAGAAG AAGAGCTCCA ATAGCCACTC GAGCAGCAGC 
AATAATCCAG TCTGCCATAC CGATAGCCAG CCCGGTGGTA CATCCCAAGC GGAGAGCGGT 
GCCATCCATG GCCACATCAG TCAGCAGCGA CAGGTGGAGC GAGAACGACA AAAGGCGAAG 
GCCGAGAAGA AGAAACCACA GAGCTTCACT TGGCCAACTG TTGTGACCGT TTTCGTTTTG 
GCCATGGGCT GTGGCTTCTT TGCGGCGCGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hid ( NM_001275081.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262010.1 Drosophila melanogaster head involution defective (hid), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18852
Clone ID Related Accession (Same CDS sequence)	NM_079412.4 , NM_001275081.1
Accession Version	NM_001275081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	head involution defective, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGTGC CCTTTTATTT GCCCGAGGGC GGCGCCGATG ACGTAGCGTC GAGTTCATCG 
GGAGCCTCGG GCAACTCCTC CCCCCACAAC CACCCACTTC CCTCGAGCGC ATCCTCGTCC 
GTCTCCTCCT CGGGCGTGTC CTCGGCCTCC GCCTCCTCGG CCTCATCTTC GTCCTCCGCA 
TCGTCGGACG GCGCCAGCAG CGCCGCCTCG CAATCGCCGA ACACCACCAC CTCGTCGGCC 
ACGCAGACGC CGATGCAGTC TCCACTGCCC ACCGACCAAG TGCTATACGC CCTCTACGAG 
TGGGTCAGGA TGTACCAGAG CCAGCAGAGT GCCCCGCAAA TCTTCCAGTA TCCGCCGCCA 
AGCCCCTCTT GCAATTTCAC GGGCGGCGAT GTGTTCTTTC CGCACGGCCA TCCGAATCCG 
AACTCGAATC CCCATCCGCG CACCCCCCGA ACCAGCGTGA GCTTCTCCTC CGGCGAGGAG 
TACAACTTCT TCCGGCAGCA GCAGCCGCAA CCACATCCGT CATATCCGGC GCCATCAACA 
CCGCAGCCAA TGCCACCGCA GTCAGCGCCG CCGATGCACT GCAGCCACAG CTACCCGCAG 
CAGTCGGCGC ACATGATGCC ACACCATTCC GCTCCCTTCG GAATGGGCGG TACCTACTAC 
GCGGGCTACA CGCCGCCACC CACTCCGAAC ACGGCCAGTG CGGGCACCTC CAGCTCATCG 
GCGGCCTTCG GCTGGCACGG CCACCCCCAC AGCCCCTTCA CGTCGACCTC CACGCCGTTA 
TCGGCGCCAG TGGCGCCCAA GATGCGCCTG CAGCGCAGCC AGTCGGATGC GGCCAGACGC 
AAGCGATTGA CCTCGACGGG CGAGGATGAG CGCGAGTACC AGAGCGATCA TGAGGCCACT 
TGGGACGAGT TTGGCGATCG CTACGACAAC TTTACGGCCG GCCGGGAGCG TCTGCAGGAG 
TTCAATGGAC GCATCCCGCC CCGGAAGAAG AAGAGCTCCA ATAGCCACTC GAGCAGCAGC 
AATAATCCAG TCTGCCATAC CGATAGCCAG CCCGGTGGTA CATCCCAAGC GGAGAGCGGT 
GCCATCCATG GCCACATCAG TCAGCAGCGA CAGGTGGAGC GAGAACGACA AAAGGCGAAG 
GCCGAGAAGA AGAAACCACA GAGCTTCACT TGGCCAACTG TTGTGACCGT TTTCGTTTTG 
GCCATGGGCT GTGGCTTCTT TGCGGCGCGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262010.1
CDS	520..1752
Translation	MAVPFYLPEGGADDVASSSSGASGNSSPHNHPLPSSASSSVSSSGVSSASASSASSSSSASSDGASSAASQSPNTTTSSATQTPMQSPLPTDQVLYALYEWVRMYQSQQSAPQIFQYPPPSPSCNFTGGDVFFPHGHPNPNSNPHPRTPRTSVSFSSGEEYNFFRQQQPQPHPSYPAPSTPQPMPPQSAPPMHCSHSYPQQSAHMMPHHSAPFGMGGTYYAGYTPPPTPNTASAGTSSSSAAFGWHGHPHSPFTSTSTPLSAPVAPKMRLQRSQSDAARRKRLTSTGEDEREYQSDHEATWDEFGDRYDNFTAGRERLQEFNGRIPPRKKKSSNSHSSSSNNPVCHTDSQPGGTSQAESGAIHGHISQQRQVERERQKAKAEKKKPQSFTWPTVVTVFVLAMGCGFFAAR

Target ORF information:

RefSeq Version	NM_001275081.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster head involution defective (hid), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275081.1

ORF Insert Sequence:

ATGGCCGTGC CCTTTTATTT GCCCGAGGGC GGCGCCGATG ACGTAGCGTC GAGTTCATCG 
GGAGCCTCGG GCAACTCCTC CCCCCACAAC CACCCACTTC CCTCGAGCGC ATCCTCGTCC 
GTCTCCTCCT CGGGCGTGTC CTCGGCCTCC GCCTCCTCGG CCTCATCTTC GTCCTCCGCA 
TCGTCGGACG GCGCCAGCAG CGCCGCCTCG CAATCGCCGA ACACCACCAC CTCGTCGGCC 
ACGCAGACGC CGATGCAGTC TCCACTGCCC ACCGACCAAG TGCTATACGC CCTCTACGAG 
TGGGTCAGGA TGTACCAGAG CCAGCAGAGT GCCCCGCAAA TCTTCCAGTA TCCGCCGCCA 
AGCCCCTCTT GCAATTTCAC GGGCGGCGAT GTGTTCTTTC CGCACGGCCA TCCGAATCCG 
AACTCGAATC CCCATCCGCG CACCCCCCGA ACCAGCGTGA GCTTCTCCTC CGGCGAGGAG 
TACAACTTCT TCCGGCAGCA GCAGCCGCAA CCACATCCGT CATATCCGGC GCCATCAACA 
CCGCAGCCAA TGCCACCGCA GTCAGCGCCG CCGATGCACT GCAGCCACAG CTACCCGCAG 
CAGTCGGCGC ACATGATGCC ACACCATTCC GCTCCCTTCG GAATGGGCGG TACCTACTAC 
GCGGGCTACA CGCCGCCACC CACTCCGAAC ACGGCCAGTG CGGGCACCTC CAGCTCATCG 
GCGGCCTTCG GCTGGCACGG CCACCCCCAC AGCCCCTTCA CGTCGACCTC CACGCCGTTA 
TCGGCGCCAG TGGCGCCCAA GATGCGCCTG CAGCGCAGCC AGTCGGATGC GGCCAGACGC 
AAGCGATTGA CCTCGACGGG CGAGGATGAG CGCGAGTACC AGAGCGATCA TGAGGCCACT 
TGGGACGAGT TTGGCGATCG CTACGACAAC TTTACGGCCG GCCGGGAGCG TCTGCAGGAG 
TTCAATGGAC GCATCCCGCC CCGGAAGAAG AAGAGCTCCA ATAGCCACTC GAGCAGCAGC 
AATAATCCAG TCTGCCATAC CGATAGCCAG CCCGGTGGTA CATCCCAAGC GGAGAGCGGT 
GCCATCCATG GCCACATCAG TCAGCAGCGA CAGGTGGAGC GAGAACGACA AAAGGCGAAG 
GCCGAGAAGA AGAAACCACA GAGCTTCACT TGGCCAACTG TTGTGACCGT TTTCGTTTTG 
GCCATGGGCT GTGGCTTCTT TGCGGCGCGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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hid cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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