Smn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smn
Entrez Gene ID	39844
Full Name	survival motor neuron
Synonyms	CG16725,DmSMN,Dmel\CG16725,SMN,dSMN,l(3)73Ao,smn
General protein information	Preferred Names survival motor neuron Names CG16725 gene product from transcript CG16725-RB CG16725-PA CG16725-PB Smn-PA Smn-PB survival of motor neurons
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 73A9-73A9

mRNA and Protein(s)

mRNA	Protein	Name
NM_079388.4	NP_524112.1	survival motor neuron, isoform A
NM_001275025.1	NP_001261954.1	survival motor neuron, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Smn	NP_524112.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004011	XP_318468.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Intragenic complementation analyses reveal the potential for dominant negative interactions between wild-type and mutant SMN subunits, highlighting the essential nature of the YG box in formation of higher-order SMN multimers.
Title: SMA-causing missense mutations in survival motor neuron (Smn) display a wide range of phenotypes when modeled in Drosophila.

Smn mutants displayed a prominent increase in levels of stress-responsive transcripts, indicating a systemic response to the developmental arrest induced by loss of SMN protein
Title: Developmental arrest of Drosophila survival motor neuron (Smn) mutants accounts for differences in expression of minor intron-containing genes.

A unique interactome for SMN provides a knowledge base for a better understanding of spinal muscular atrophy.
Title: Genetic circuitry of Survival motor neuron, the gene underlying spinal muscular atrophy.

Our results uncover a hitherto under-appreciated role for SMN in homeostatic regulatory mechanisms at motor synapses.
Title: Behavioral and electrophysiological outcomes of tissue-specific Smn knockdown in Drosophila melanogaster.

Study shows that SMN deficiency perturbs splicing and decreases the expression of a subset of U12 intron-containing genes in mammalian cells and Drosophila larvae.
Title: An SMN-dependent U12 splicing event essential for motor circuit function.

The following Smn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18714	NM_001275025.1 Latest version!	Drosophila melanogaster survival motor neuron (Smn), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa18714	NM_079388.4 Latest version!	Drosophila melanogaster survival motor neuron (Smn), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smn ( NM_079388.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524112.1 Drosophila melanogaster survival motor neuron (Smn), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18714
Clone ID Related Accession (Same CDS sequence)	NM_079388.4 , NM_001275025.1
Accession Version	NM_079388.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	survival motor neuron, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079388.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGACG AGACGAACGC CGCGGTGTGG GATGACTCCT TGCTGGTGAA GACGTACGAC 
GAGTCGGTGG GACTGGCCCG CGAAGCTTTG GCCCGCCGCC TCGCCGATTC CACCAACAAG 
CGCGAGGAGG AGAACGCTGC CGCAGCTGAA GAAGAAGCTG GCGAGATCTC GGCCACTGGT 
GGGGCTACCA GCCCGGAACC AGTATCCTTC AAAGTAGGCG ACTACGCCAG GGCCACCTAC 
GTGGATGGTG TGGACTACGA GGGCGCTGTG GTGTCTATTA ACGAGGAGAA GGGCACCTGC 
GTACTCCGCT ATTTGGGCTA TGAGAACGAG CAGGAGGTGT TGCTCGTGGA CCTCCTGCCG 
TCCTGGGGCA AGCGTGTGCG TCGCGAGCAG TTCCTTATCG CCAAGAAGGA TGAGGACGAG 
CAGTTGAGCC GCCCTAAGGC ATCCGCTGGT AGTCATTCAA AGACTCCCAA GTCATCCAGA 
AGGAGTAGGA TCTCTGGCGG ACTGGTTATG CCGCCCATGC CACCAGTACC GCCCATGATT 
GTCGGACAAG GGGACGGCGC CGAACAGGAC TTCGTGGCCA TGCTCACCGC CTGGTACATG 
TCCGGCTATT ATACGGGCCT CTACCAGGGA AAGAAAGAAG CCAGCACCAC TAGCGGAAAG 
AAAAAGACAC CCAAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524112.1
CDS	95..775
Translation	MSDETNAAVWDDSLLVKTYDESVGLAREALARRLADSTNKREEENAAAAEEEAGEISATGGATSPEPVSFKVGDYARATYVDGVDYEGAVVSINEEKGTCVLRYLGYENEQEVLLVDLLPSWGKRVRREQFLIAKKDEDEQLSRPKASAGSHSKTPKSSRRSRISGGLVMPPMPPVPPMIVGQGDGAEQDFVAMLTAWYMSGYYTGLYQGKKEASTTSGKKKTPKK

Target ORF information:

RefSeq Version	NM_079388.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster survival motor neuron (Smn), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079388.4

ORF Insert Sequence:

ATGTCCGACG AGACGAACGC CGCGGTGTGG GATGACTCCT TGCTGGTGAA GACGTACGAC 
GAGTCGGTGG GACTGGCCCG CGAAGCTTTG GCCCGCCGCC TCGCCGATTC CACCAACAAG 
CGCGAGGAGG AGAACGCTGC CGCAGCTGAA GAAGAAGCTG GCGAGATCTC GGCCACTGGT 
GGGGCTACCA GCCCGGAACC AGTATCCTTC AAAGTAGGCG ACTACGCCAG GGCCACCTAC 
GTGGATGGTG TGGACTACGA GGGCGCTGTG GTGTCTATTA ACGAGGAGAA GGGCACCTGC 
GTACTCCGCT ATTTGGGCTA TGAGAACGAG CAGGAGGTGT TGCTCGTGGA CCTCCTGCCG 
TCCTGGGGCA AGCGTGTGCG TCGCGAGCAG TTCCTTATCG CCAAGAAGGA TGAGGACGAG 
CAGTTGAGCC GCCCTAAGGC ATCCGCTGGT AGTCATTCAA AGACTCCCAA GTCATCCAGA 
AGGAGTAGGA TCTCTGGCGG ACTGGTTATG CCGCCCATGC CACCAGTACC GCCCATGATT 
GTCGGACAAG GGGACGGCGC CGAACAGGAC TTCGTGGCCA TGCTCACCGC CTGGTACATG 
TCCGGCTATT ATACGGGCCT CTACCAGGGA AAGAAAGAAG CCAGCACCAC TAGCGGAAAG 
AAAAAGACAC CCAAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smn ( NM_001275025.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261954.1 Drosophila melanogaster survival motor neuron (Smn), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18714
Clone ID Related Accession (Same CDS sequence)	NM_079388.4 , NM_001275025.1
Accession Version	NM_001275025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	survival motor neuron, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGACG AGACGAACGC CGCGGTGTGG GATGACTCCT TGCTGGTGAA GACGTACGAC 
GAGTCGGTGG GACTGGCCCG CGAAGCTTTG GCCCGCCGCC TCGCCGATTC CACCAACAAG 
CGCGAGGAGG AGAACGCTGC CGCAGCTGAA GAAGAAGCTG GCGAGATCTC GGCCACTGGT 
GGGGCTACCA GCCCGGAACC AGTATCCTTC AAAGTAGGCG ACTACGCCAG GGCCACCTAC 
GTGGATGGTG TGGACTACGA GGGCGCTGTG GTGTCTATTA ACGAGGAGAA GGGCACCTGC 
GTACTCCGCT ATTTGGGCTA TGAGAACGAG CAGGAGGTGT TGCTCGTGGA CCTCCTGCCG 
TCCTGGGGCA AGCGTGTGCG TCGCGAGCAG TTCCTTATCG CCAAGAAGGA TGAGGACGAG 
CAGTTGAGCC GCCCTAAGGC ATCCGCTGGT AGTCATTCAA AGACTCCCAA GTCATCCAGA 
AGGAGTAGGA TCTCTGGCGG ACTGGTTATG CCGCCCATGC CACCAGTACC GCCCATGATT 
GTCGGACAAG GGGACGGCGC CGAACAGGAC TTCGTGGCCA TGCTCACCGC CTGGTACATG 
TCCGGCTATT ATACGGGCCT CTACCAGGGA AAGAAAGAAG CCAGCACCAC TAGCGGAAAG 
AAAAAGACAC CCAAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261954.1
CDS	302..982
Translation	MSDETNAAVWDDSLLVKTYDESVGLAREALARRLADSTNKREEENAAAAEEEAGEISATGGATSPEPVSFKVGDYARATYVDGVDYEGAVVSINEEKGTCVLRYLGYENEQEVLLVDLLPSWGKRVRREQFLIAKKDEDEQLSRPKASAGSHSKTPKSSRRSRISGGLVMPPMPPVPPMIVGQGDGAEQDFVAMLTAWYMSGYYTGLYQGKKEASTTSGKKKTPKK

Target ORF information:

RefSeq Version	NM_001275025.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster survival motor neuron (Smn), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275025.1

ORF Insert Sequence:

ATGTCCGACG AGACGAACGC CGCGGTGTGG GATGACTCCT TGCTGGTGAA GACGTACGAC 
GAGTCGGTGG GACTGGCCCG CGAAGCTTTG GCCCGCCGCC TCGCCGATTC CACCAACAAG 
CGCGAGGAGG AGAACGCTGC CGCAGCTGAA GAAGAAGCTG GCGAGATCTC GGCCACTGGT 
GGGGCTACCA GCCCGGAACC AGTATCCTTC AAAGTAGGCG ACTACGCCAG GGCCACCTAC 
GTGGATGGTG TGGACTACGA GGGCGCTGTG GTGTCTATTA ACGAGGAGAA GGGCACCTGC 
GTACTCCGCT ATTTGGGCTA TGAGAACGAG CAGGAGGTGT TGCTCGTGGA CCTCCTGCCG 
TCCTGGGGCA AGCGTGTGCG TCGCGAGCAG TTCCTTATCG CCAAGAAGGA TGAGGACGAG 
CAGTTGAGCC GCCCTAAGGC ATCCGCTGGT AGTCATTCAA AGACTCCCAA GTCATCCAGA 
AGGAGTAGGA TCTCTGGCGG ACTGGTTATG CCGCCCATGC CACCAGTACC GCCCATGATT 
GTCGGACAAG GGGACGGCGC CGAACAGGAC TTCGTGGCCA TGCTCACCGC CTGGTACATG 
TCCGGCTATT ATACGGGCCT CTACCAGGGA AAGAAAGAAG CCAGCACCAC TAGCGGAAAG 
AAAAAGACAC CCAAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Smn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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