RAF2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAF2
Entrez Gene ID	39821
Full Name	RING-associated factor 2
Synonyms	CG4689,CG4877,Dmel\CG4877,dRAF2
General protein information	Preferred Names RING-associated factor 2 Names CG4877 gene product from transcript CG4877-RA CG4877-PA CG4877-PB CG4877-PC RAF2-PA RAF2-PB RAF2-PC dRING-associated factor 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 72E4-72E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275014.1	NP_001261943.1	RING-associated factor 2, isoform C
NM_140632.3	NP_648889.2	RING-associated factor 2, isoform B
NM_168664.2	NP_730163.1	RING-associated factor 2, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RAF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18692	NM_140632.3 Latest version!	Drosophila melanogaster RING-associated factor 2 (RAF2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa18692	NM_168664.2 Latest version!	Drosophila melanogaster RING-associated factor 2 (RAF2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa18692	NM_001275014.1 Latest version!	Drosophila melanogaster RING-associated factor 2 (RAF2), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAF2 ( NM_001275014.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261943.1 Drosophila melanogaster RING-associated factor 2 (RAF2), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa18692
Clone ID Related Accession (Same CDS sequence)	NM_001275014.1 , NM_140632.3 , NM_168664.2
Accession Version	NM_001275014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RING-associated factor 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAACG AGATCTACGA CTCCTCCAAT TCGCAGCTAA GTAGCGACGA GTATAGCCAC 
GAGGAGGAGG AATACGACGC TCTAATGGAC GCGGCGGTGA GCAAGCAACG ACCCCAAAAG 
ACAAAATCCA AGGGATCCGA GTCCGAGAAA CCCGGAAATG GAGCTCCAGA ATCGGCGGCT 
GCCACTGAGA AAAATGAAAA GCCAGATGAC GATGCGGTGC CACCTTCCAC GCGCTCAGAA 
ACAATTCGGC GGATCCGGCA ATTGCAGTTA CAGCGTGGCA GGCTGCCCAA TCTTTACTTC 
AAGCGTCGCA GTATTCGTTG GCTGCGACCC AATAAAATGG ACTACAACAG CTATTTTGAC 
GGCCTGAAGG ACTTGCTGCC CAAGCGTAAA TTGAAGCACC TGGTTCGCTT CCATGACAGG 
CAGCGCAGAA CCTACGACAG CAGGAGCATC AGTCGTAGTC GCAGCCACAG CCGCAGTCTT 
AGCCGAAGTT GCAGTCGCAG TCGTAGTCGT AGCTATTCTC GCAGCCGCAG CGGCAGTGGT 
TCGCCGGAAC TCATTTGCCT TGACGACACG GAGAACGAGG ATTCGCCTGA GAAACCAGAG 
CAAGAGCCGG CAAGGGTTAC GCCCATCAAG GAAAATAAGC CTACGCTTCC GGCTCCACCC 
CAACTAAACT ACGAGCTGCC AGAAAGGAAG AAAGCAAACC CACTTGGAAA CAACGAAAAC 
GGTGCCTCTA TCCTCGACGA GTTCCTGGTA AAGAAGGATC CCCAGGAACC GGTCTTCGAC 
TACGGTAAAC TCTCAGCGTG TCGGGAATTG GAACAGGCTC TAAAGGACCG GTCGCGAGAT 
GCGCCAAATG AGGCAGAGTC GCTTGTGGTT GATGCCACTC TTAAAAGACG CCGTTCGGTA 
ACACCGCCCG CTAGCGAAGA GAAGCGCAAC AATGAAGTCG AGGATGATAA TATAATTGGG 
TTTTCAACGG TGCAGCAACA GAAGCCAATG GAGGCAGCTC CACCATTAAA TCTAAGAATG 
CCCACCACTC CATCGACTAA TCCTCTTCAG CGTCAGCAGG CTTTTAAGCT GAGCACACCG 
TATACCCTGG CTCCGCCTAC TGCCGCCAAG CAAGCTGACA CTTACAGTTT AAATACTCCA 
CCGCCAACGG CCACCCAAAT GCAGATATCC TATCCCAACG AAAAGCTGTC AAATAACATG 
AAAGCTAATG CGACTTTCTC GATTCAACAG ACAGCTTTAT CCCAAGTCGC TGCATCCTAT 
GCTGCTGCAC CACAAGTGGC TGCCCTCGCG CAGAGAGCTC CATCTCAGGT TGCTCCACTA 
CAACAAGCCC CCGTGCAACA GATGCACTTC CCAAGATCAG TTCCACCGCA GCAGCTTCCT 
CCACCACAGC AGCGAGCTCC TCCACAGCAA CTGGCTACAG CTCCATTCGC TCCACCACGG 
CACCTGGCTC AACATAGACC ATCTCCACAG CAGCTAGCTC CATCACAGCA GCTGGCTCCA 
CCACACCAGC TAGCTCCACC ACACCAGCTA GCTCCACCAC AGCAGCGAGC TCCGCCACAG 
CAGCTAGCTC CACCACACCA GCTAGCTCCA CCACACCAGC TAGCTCCACC ACAGCAGCGA 
GCTCCGCCAC AGCAGCTAGC TCCGCCACAG CAGCTAGCTC CACCACAGCA GCTAGCTCCA 
CCACAGCAGC TAGCTCCACC ACAGCAGCTA GCTCCACCAC AGCAGCGAGC TCCGCCACAG 
CAGCGAGCTC CGCCACAGCA GCTGGCTCCG CCACAACAGC GAGCTCCCAC ACAGCATATG 
GCTCCACCAC AACATATGGC TCCGCCACAG CAGCTAGCTC CAACACAGCA GCTAGCTCCT 
CCACCACAGC AGCTAGCGCC TCGACCACAG CAGCTAGCTC CTCCACCACA GCAACTAGCT 
CCTCCACCAC AGCAGCTAGC TCCTCCACCA CAGCAGCCAG ATTCATCAAG ACCAGCTTCG 
GTGCAGCCGT TGACCCCTCA AAGGCATGCT AGTCCACATC AGCTTGCCCA ATCACAGCAG 
GTCCATCAAT CGCCATCTCA ACTGCATCCT GTTCCAGTGG CTGCTCCCAA CTTTGCGGTG 
CCCCAACAGC CGCAACCCCG TCGATCGGAA ACGGTGAGCA CCCAGACGCA GGGCTTGGGG 
TCACCAGCGG CTTCTTCTTC GACAACCCGC CAAAAGTCAT TCGTTGATCT CAATAATAGT 
GATGCAAACT TCCATTATCG CGTCAAAGAA CTTTTCGATG AATTGAACTC GACAATGATT 
GATAAAATTA ACTCGGTACC CCCTGATGAA GATTCATTGA AAAAAGAGCG CGAACGCATC 
GTTGCGGATC TTGCAGCCCT AGATAAGCTG ATGGCCCAGA AAGAGGATGA ATATAACCGA 
CTGCTGTATT TGAGCTGCAT TAAGAAGGAG CTGCATGAAC GAATGGAGCG AAAGGAGCGC 
TTGATACGTA TTAAGGACCT ACTGCCCATC CTTATCAACA AGTGCGGCAC CAAGGAGCTC 
AGTGAGGTAA AGGCTATGCT AGAAGAGGAA ATCGATTCTC CAATGGCCTC CAAACATAGT 
TTGTCGGCAA TTGAAAAATT ACTGAATTAC GCCGAGTTAA ACCAGAACAT CATACAGCTT 
CTGCGGGGTT CCTTGGGCTT AAATAAACGC GCACCGTCCG TGTCTCCGGC CAATTTCGAG 
GATGACCAAA TGCTTTTTCG TAGAGATTCC TTGCCCGTAA GTCATCTATC AATGCGGGAT 
CAGCATTACG AATCTCGGAA TGCGGTAAAT GACCATCTGC TAGATAGCAA TCCAGACAGA 
GATCCGCCAG CCAAGCGACC GAAGCTAATC CACTCGCAAG ACAGTAACCA GGACATGGCA 
AGCTCCTCGA CACATTTGTC AGCCAATCTT TCAGAGACGC TTCGCAAGAT TAGGGCTTGG 
AAAAATTATT CGCATTTTAG TAACGTCTCA ATGGAGGACA ATAATTCGGA TAATGAATCA 
CAGATCGAAC CGCTGTTCAA CAGCAGTCCG ATGGCCTCCC GTCGCCAACT GGCCAATAGA 
CAGTCCTACC AAGAACAACA TTCCGCTAAT ATCAACGACC ATGAGAATGT CCAAAAGAGG 
CACAAGTCTC ACAAGCGCAA GTCGCATAAG CACAAGTCTC ACAAGCATAG GAGCTCCCAT 
AAGTCCCAGG CTGAATCTTC CGCCACCATC AACGGTAACA CAGGTAGACA ATGCCACGAG 
TGCAAGCTCT ATGGAGCCAC CTTTATGTGC TCCAACTGTC AGAATCAATG GTACTGCAGT 
CGTGAATGCC AGCTGAGCGA TTGGGACACA CATCACCGAA CTTGTGGCAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261943.1
CDS	250..3603
Translation	MSNEIYDSSNSQLSSDEYSHEEEEYDALMDAAVSKQRPQKTKSKGSESEKPGNGAPESAAATEKNEKPDDDAVPPSTRSETIRRIRQLQLQRGRLPNLYFKRRSIRWLRPNKMDYNSYFDGLKDLLPKRKLKHLVRFHDRQRRTYDSRSISRSRSHSRSLSRSCSRSRSRSYSRSRSGSGSPELICLDDTENEDSPEKPEQEPARVTPIKENKPTLPAPPQLNYELPERKKANPLGNNENGASILDEFLVKKDPQEPVFDYGKLSACRELEQALKDRSRDAPNEAESLVVDATLKRRRSVTPPASEEKRNNEVEDDNIIGFSTVQQQKPMEAAPPLNLRMPTTPSTNPLQRQQAFKLSTPYTLAPPTAAKQADTYSLNTPPPTATQMQISYPNEKLSNNMKANATFSIQQTALSQVAASYAAAPQVAALAQRAPSQVAPLQQAPVQQMHFPRSVPPQQLPPPQQRAPPQQLATAPFAPPRHLAQHRPSPQQLAPSQQLAPPHQLAPPHQLAPPQQRAPPQQLAPPHQLAPPHQLAPPQQRAPPQQLAPPQQLAPPQQLAPPQQLAPPQQLAPPQQRAPPQQRAPPQQLAPPQQRAPTQHMAPPQHMAPPQQLAPTQQLAPPPQQLAPRPQQLAPPPQQLAPPPQQLAPPPQQPDSSRPASVQPLTPQRHASPHQLAQSQQVHQSPSQLHPVPVAAPNFAVPQQPQPRRSETVSTQTQGLGSPAASSSTTRQKSFVDLNNSDANFHYRVKELFDELNSTMIDKINSVPPDEDSLKKERERIVADLAALDKLMAQKEDEYNRLLYLSCIKKELHERMERKERLIRIKDLLPILINKCGTKELSEVKAMLEEEIDSPMASKHSLSAIEKLLNYAELNQNIIQLLRGSLGLNKRAPSVSPANFEDDQMLFRRDSLPVSHLSMRDQHYESRNAVNDHLLDSNPDRDPPAKRPKLIHSQDSNQDMASSSTHLSANLSETLRKIRAWKNYSHFSNVSMEDNNSDNESQIEPLFNSSPMASRRQLANRQSYQEQHSANINDHENVQKRHKSHKRKSHKHKSHKHRSSHKSQAESSATINGNTGRQCHECKLYGATFMCSNCQNQWYCSRECQLSDWDTHHRTCGI

Target ORF information:

RefSeq Version	NM_001275014.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RING-associated factor 2 (RAF2), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275014.1

ORF Insert Sequence:

ATGTCCAACG AGATCTACGA CTCCTCCAAT TCGCAGCTAA GTAGCGACGA GTATAGCCAC 
GAGGAGGAGG AATACGACGC TCTAATGGAC GCGGCGGTGA GCAAGCAACG ACCCCAAAAG 
ACAAAATCCA AGGGATCCGA GTCCGAGAAA CCCGGAAATG GAGCTCCAGA ATCGGCGGCT 
GCCACTGAGA AAAATGAAAA GCCAGATGAC GATGCGGTGC CACCTTCCAC GCGCTCAGAA 
ACAATTCGGC GGATCCGGCA ATTGCAGTTA CAGCGTGGCA GGCTGCCCAA TCTTTACTTC 
AAGCGTCGCA GTATTCGTTG GCTGCGACCC AATAAAATGG ACTACAACAG CTATTTTGAC 
GGCCTGAAGG ACTTGCTGCC CAAGCGTAAA TTGAAGCACC TGGTTCGCTT CCATGACAGG 
CAGCGCAGAA CCTACGACAG CAGGAGCATC AGTCGTAGTC GCAGCCACAG CCGCAGTCTT 
AGCCGAAGTT GCAGTCGCAG TCGTAGTCGT AGCTATTCTC GCAGCCGCAG CGGCAGTGGT 
TCGCCGGAAC TCATTTGCCT TGACGACACG GAGAACGAGG ATTCGCCTGA GAAACCAGAG 
CAAGAGCCGG CAAGGGTTAC GCCCATCAAG GAAAATAAGC CTACGCTTCC GGCTCCACCC 
CAACTAAACT ACGAGCTGCC AGAAAGGAAG AAAGCAAACC CACTTGGAAA CAACGAAAAC 
GGTGCCTCTA TCCTCGACGA GTTCCTGGTA AAGAAGGATC CCCAGGAACC GGTCTTCGAC 
TACGGTAAAC TCTCAGCGTG TCGGGAATTG GAACAGGCTC TAAAGGACCG GTCGCGAGAT 
GCGCCAAATG AGGCAGAGTC GCTTGTGGTT GATGCCACTC TTAAAAGACG CCGTTCGGTA 
ACACCGCCCG CTAGCGAAGA GAAGCGCAAC AATGAAGTCG AGGATGATAA TATAATTGGG 
TTTTCAACGG TGCAGCAACA GAAGCCAATG GAGGCAGCTC CACCATTAAA TCTAAGAATG 
CCCACCACTC CATCGACTAA TCCTCTTCAG CGTCAGCAGG CTTTTAAGCT GAGCACACCG 
TATACCCTGG CTCCGCCTAC TGCCGCCAAG CAAGCTGACA CTTACAGTTT AAATACTCCA 
CCGCCAACGG CCACCCAAAT GCAGATATCC TATCCCAACG AAAAGCTGTC AAATAACATG 
AAAGCTAATG CGACTTTCTC GATTCAACAG ACAGCTTTAT CCCAAGTCGC TGCATCCTAT 
GCTGCTGCAC CACAAGTGGC TGCCCTCGCG CAGAGAGCTC CATCTCAGGT TGCTCCACTA 
CAACAAGCCC CCGTGCAACA GATGCACTTC CCAAGATCAG TTCCACCGCA GCAGCTTCCT 
CCACCACAGC AGCGAGCTCC TCCACAGCAA CTGGCTACAG CTCCATTCGC TCCACCACGG 
CACCTGGCTC AACATAGACC ATCTCCACAG CAGCTAGCTC CATCACAGCA GCTGGCTCCA 
CCACACCAGC TAGCTCCACC ACACCAGCTA GCTCCACCAC AGCAGCGAGC TCCGCCACAG 
CAGCTAGCTC CACCACACCA GCTAGCTCCA CCACACCAGC TAGCTCCACC ACAGCAGCGA 
GCTCCGCCAC AGCAGCTAGC TCCGCCACAG CAGCTAGCTC CACCACAGCA GCTAGCTCCA 
CCACAGCAGC TAGCTCCACC ACAGCAGCTA GCTCCACCAC AGCAGCGAGC TCCGCCACAG 
CAGCGAGCTC CGCCACAGCA GCTGGCTCCG CCACAACAGC GAGCTCCCAC ACAGCATATG 
GCTCCACCAC AACATATGGC TCCGCCACAG CAGCTAGCTC CAACACAGCA GCTAGCTCCT 
CCACCACAGC AGCTAGCGCC TCGACCACAG CAGCTAGCTC CTCCACCACA GCAACTAGCT 
CCTCCACCAC AGCAGCTAGC TCCTCCACCA CAGCAGCCAG ATTCATCAAG ACCAGCTTCG 
GTGCAGCCGT TGACCCCTCA AAGGCATGCT AGTCCACATC AGCTTGCCCA ATCACAGCAG 
GTCCATCAAT CGCCATCTCA ACTGCATCCT GTTCCAGTGG CTGCTCCCAA CTTTGCGGTG 
CCCCAACAGC CGCAACCCCG TCGATCGGAA ACGGTGAGCA CCCAGACGCA GGGCTTGGGG 
TCACCAGCGG CTTCTTCTTC GACAACCCGC CAAAAGTCAT TCGTTGATCT CAATAATAGT 
GATGCAAACT TCCATTATCG CGTCAAAGAA CTTTTCGATG AATTGAACTC GACAATGATT 
GATAAAATTA ACTCGGTACC CCCTGATGAA GATTCATTGA AAAAAGAGCG CGAACGCATC 
GTTGCGGATC TTGCAGCCCT AGATAAGCTG ATGGCCCAGA AAGAGGATGA ATATAACCGA 
CTGCTGTATT TGAGCTGCAT TAAGAAGGAG CTGCATGAAC GAATGGAGCG AAAGGAGCGC 
TTGATACGTA TTAAGGACCT ACTGCCCATC CTTATCAACA AGTGCGGCAC CAAGGAGCTC 
AGTGAGGTAA AGGCTATGCT AGAAGAGGAA ATCGATTCTC CAATGGCCTC CAAACATAGT 
TTGTCGGCAA TTGAAAAATT ACTGAATTAC GCCGAGTTAA ACCAGAACAT CATACAGCTT 
CTGCGGGGTT CCTTGGGCTT AAATAAACGC GCACCGTCCG TGTCTCCGGC CAATTTCGAG 
GATGACCAAA TGCTTTTTCG TAGAGATTCC TTGCCCGTAA GTCATCTATC AATGCGGGAT 
CAGCATTACG AATCTCGGAA TGCGGTAAAT GACCATCTGC TAGATAGCAA TCCAGACAGA 
GATCCGCCAG CCAAGCGACC GAAGCTAATC CACTCGCAAG ACAGTAACCA GGACATGGCA 
AGCTCCTCGA CACATTTGTC AGCCAATCTT TCAGAGACGC TTCGCAAGAT TAGGGCTTGG 
AAAAATTATT CGCATTTTAG TAACGTCTCA ATGGAGGACA ATAATTCGGA TAATGAATCA 
CAGATCGAAC CGCTGTTCAA CAGCAGTCCG ATGGCCTCCC GTCGCCAACT GGCCAATAGA 
CAGTCCTACC AAGAACAACA TTCCGCTAAT ATCAACGACC ATGAGAATGT CCAAAAGAGG 
CACAAGTCTC ACAAGCGCAA GTCGCATAAG CACAAGTCTC ACAAGCATAG GAGCTCCCAT 
AAGTCCCAGG CTGAATCTTC CGCCACCATC AACGGTAACA CAGGTAGACA ATGCCACGAG 
TGCAAGCTCT ATGGAGCCAC CTTTATGTGC TCCAACTGTC AGAATCAATG GTACTGCAGT 
CGTGAATGCC AGCTGAGCGA TTGGGACACA CATCACCGAA CTTGTGGCAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAF2 ( NM_140632.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648889.2 Drosophila melanogaster RING-associated factor 2 (RAF2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18692
Clone ID Related Accession (Same CDS sequence)	NM_001275014.1 , NM_140632.3 , NM_168664.2
Accession Version	NM_140632.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RING-associated factor 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140632.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAACG AGATCTACGA CTCCTCCAAT TCGCAGCTAA GTAGCGACGA GTATAGCCAC 
GAGGAGGAGG AATACGACGC TCTAATGGAC GCGGCGGTGA GCAAGCAACG ACCCCAAAAG 
ACAAAATCCA AGGGATCCGA GTCCGAGAAA CCCGGAAATG GAGCTCCAGA ATCGGCGGCT 
GCCACTGAGA AAAATGAAAA GCCAGATGAC GATGCGGTGC CACCTTCCAC GCGCTCAGAA 
ACAATTCGGC GGATCCGGCA ATTGCAGTTA CAGCGTGGCA GGCTGCCCAA TCTTTACTTC 
AAGCGTCGCA GTATTCGTTG GCTGCGACCC AATAAAATGG ACTACAACAG CTATTTTGAC 
GGCCTGAAGG ACTTGCTGCC CAAGCGTAAA TTGAAGCACC TGGTTCGCTT CCATGACAGG 
CAGCGCAGAA CCTACGACAG CAGGAGCATC AGTCGTAGTC GCAGCCACAG CCGCAGTCTT 
AGCCGAAGTT GCAGTCGCAG TCGTAGTCGT AGCTATTCTC GCAGCCGCAG CGGCAGTGGT 
TCGCCGGAAC TCATTTGCCT TGACGACACG GAGAACGAGG ATTCGCCTGA GAAACCAGAG 
CAAGAGCCGG CAAGGGTTAC GCCCATCAAG GAAAATAAGC CTACGCTTCC GGCTCCACCC 
CAACTAAACT ACGAGCTGCC AGAAAGGAAG AAAGCAAACC CACTTGGAAA CAACGAAAAC 
GGTGCCTCTA TCCTCGACGA GTTCCTGGTA AAGAAGGATC CCCAGGAACC GGTCTTCGAC 
TACGGTAAAC TCTCAGCGTG TCGGGAATTG GAACAGGCTC TAAAGGACCG GTCGCGAGAT 
GCGCCAAATG AGGCAGAGTC GCTTGTGGTT GATGCCACTC TTAAAAGACG CCGTTCGGTA 
ACACCGCCCG CTAGCGAAGA GAAGCGCAAC AATGAAGTCG AGGATGATAA TATAATTGGG 
TTTTCAACGG TGCAGCAACA GAAGCCAATG GAGGCAGCTC CACCATTAAA TCTAAGAATG 
CCCACCACTC CATCGACTAA TCCTCTTCAG CGTCAGCAGG CTTTTAAGCT GAGCACACCG 
TATACCCTGG CTCCGCCTAC TGCCGCCAAG CAAGCTGACA CTTACAGTTT AAATACTCCA 
CCGCCAACGG CCACCCAAAT GCAGATATCC TATCCCAACG AAAAGCTGTC AAATAACATG 
AAAGCTAATG CGACTTTCTC GATTCAACAG ACAGCTTTAT CCCAAGTCGC TGCATCCTAT 
GCTGCTGCAC CACAAGTGGC TGCCCTCGCG CAGAGAGCTC CATCTCAGGT TGCTCCACTA 
CAACAAGCCC CCGTGCAACA GATGCACTTC CCAAGATCAG TTCCACCGCA GCAGCTTCCT 
CCACCACAGC AGCGAGCTCC TCCACAGCAA CTGGCTACAG CTCCATTCGC TCCACCACGG 
CACCTGGCTC AACATAGACC ATCTCCACAG CAGCTAGCTC CATCACAGCA GCTGGCTCCA 
CCACACCAGC TAGCTCCACC ACACCAGCTA GCTCCACCAC AGCAGCGAGC TCCGCCACAG 
CAGCTAGCTC CACCACACCA GCTAGCTCCA CCACACCAGC TAGCTCCACC ACAGCAGCGA 
GCTCCGCCAC AGCAGCTAGC TCCGCCACAG CAGCTAGCTC CACCACAGCA GCTAGCTCCA 
CCACAGCAGC TAGCTCCACC ACAGCAGCTA GCTCCACCAC AGCAGCGAGC TCCGCCACAG 
CAGCGAGCTC CGCCACAGCA GCTGGCTCCG CCACAACAGC GAGCTCCCAC ACAGCATATG 
GCTCCACCAC AACATATGGC TCCGCCACAG CAGCTAGCTC CAACACAGCA GCTAGCTCCT 
CCACCACAGC AGCTAGCGCC TCGACCACAG CAGCTAGCTC CTCCACCACA GCAACTAGCT 
CCTCCACCAC AGCAGCTAGC TCCTCCACCA CAGCAGCCAG ATTCATCAAG ACCAGCTTCG 
GTGCAGCCGT TGACCCCTCA AAGGCATGCT AGTCCACATC AGCTTGCCCA ATCACAGCAG 
GTCCATCAAT CGCCATCTCA ACTGCATCCT GTTCCAGTGG CTGCTCCCAA CTTTGCGGTG 
CCCCAACAGC CGCAACCCCG TCGATCGGAA ACGGTGAGCA CCCAGACGCA GGGCTTGGGG 
TCACCAGCGG CTTCTTCTTC GACAACCCGC CAAAAGTCAT TCGTTGATCT CAATAATAGT 
GATGCAAACT TCCATTATCG CGTCAAAGAA CTTTTCGATG AATTGAACTC GACAATGATT 
GATAAAATTA ACTCGGTACC CCCTGATGAA GATTCATTGA AAAAAGAGCG CGAACGCATC 
GTTGCGGATC TTGCAGCCCT AGATAAGCTG ATGGCCCAGA AAGAGGATGA ATATAACCGA 
CTGCTGTATT TGAGCTGCAT TAAGAAGGAG CTGCATGAAC GAATGGAGCG AAAGGAGCGC 
TTGATACGTA TTAAGGACCT ACTGCCCATC CTTATCAACA AGTGCGGCAC CAAGGAGCTC 
AGTGAGGTAA AGGCTATGCT AGAAGAGGAA ATCGATTCTC CAATGGCCTC CAAACATAGT 
TTGTCGGCAA TTGAAAAATT ACTGAATTAC GCCGAGTTAA ACCAGAACAT CATACAGCTT 
CTGCGGGGTT CCTTGGGCTT AAATAAACGC GCACCGTCCG TGTCTCCGGC CAATTTCGAG 
GATGACCAAA TGCTTTTTCG TAGAGATTCC TTGCCCGTAA GTCATCTATC AATGCGGGAT 
CAGCATTACG AATCTCGGAA TGCGGTAAAT GACCATCTGC TAGATAGCAA TCCAGACAGA 
GATCCGCCAG CCAAGCGACC GAAGCTAATC CACTCGCAAG ACAGTAACCA GGACATGGCA 
AGCTCCTCGA CACATTTGTC AGCCAATCTT TCAGAGACGC TTCGCAAGAT TAGGGCTTGG 
AAAAATTATT CGCATTTTAG TAACGTCTCA ATGGAGGACA ATAATTCGGA TAATGAATCA 
CAGATCGAAC CGCTGTTCAA CAGCAGTCCG ATGGCCTCCC GTCGCCAACT GGCCAATAGA 
CAGTCCTACC AAGAACAACA TTCCGCTAAT ATCAACGACC ATGAGAATGT CCAAAAGAGG 
CACAAGTCTC ACAAGCGCAA GTCGCATAAG CACAAGTCTC ACAAGCATAG GAGCTCCCAT 
AAGTCCCAGG CTGAATCTTC CGCCACCATC AACGGTAACA CAGGTAGACA ATGCCACGAG 
TGCAAGCTCT ATGGAGCCAC CTTTATGTGC TCCAACTGTC AGAATCAATG GTACTGCAGT 
CGTGAATGCC AGCTGAGCGA TTGGGACACA CATCACCGAA CTTGTGGCAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648889.2
CDS	180..3533
Translation	MSNEIYDSSNSQLSSDEYSHEEEEYDALMDAAVSKQRPQKTKSKGSESEKPGNGAPESAAATEKNEKPDDDAVPPSTRSETIRRIRQLQLQRGRLPNLYFKRRSIRWLRPNKMDYNSYFDGLKDLLPKRKLKHLVRFHDRQRRTYDSRSISRSRSHSRSLSRSCSRSRSRSYSRSRSGSGSPELICLDDTENEDSPEKPEQEPARVTPIKENKPTLPAPPQLNYELPERKKANPLGNNENGASILDEFLVKKDPQEPVFDYGKLSACRELEQALKDRSRDAPNEAESLVVDATLKRRRSVTPPASEEKRNNEVEDDNIIGFSTVQQQKPMEAAPPLNLRMPTTPSTNPLQRQQAFKLSTPYTLAPPTAAKQADTYSLNTPPPTATQMQISYPNEKLSNNMKANATFSIQQTALSQVAASYAAAPQVAALAQRAPSQVAPLQQAPVQQMHFPRSVPPQQLPPPQQRAPPQQLATAPFAPPRHLAQHRPSPQQLAPSQQLAPPHQLAPPHQLAPPQQRAPPQQLAPPHQLAPPHQLAPPQQRAPPQQLAPPQQLAPPQQLAPPQQLAPPQQLAPPQQRAPPQQRAPPQQLAPPQQRAPTQHMAPPQHMAPPQQLAPTQQLAPPPQQLAPRPQQLAPPPQQLAPPPQQLAPPPQQPDSSRPASVQPLTPQRHASPHQLAQSQQVHQSPSQLHPVPVAAPNFAVPQQPQPRRSETVSTQTQGLGSPAASSSTTRQKSFVDLNNSDANFHYRVKELFDELNSTMIDKINSVPPDEDSLKKERERIVADLAALDKLMAQKEDEYNRLLYLSCIKKELHERMERKERLIRIKDLLPILINKCGTKELSEVKAMLEEEIDSPMASKHSLSAIEKLLNYAELNQNIIQLLRGSLGLNKRAPSVSPANFEDDQMLFRRDSLPVSHLSMRDQHYESRNAVNDHLLDSNPDRDPPAKRPKLIHSQDSNQDMASSSTHLSANLSETLRKIRAWKNYSHFSNVSMEDNNSDNESQIEPLFNSSPMASRRQLANRQSYQEQHSANINDHENVQKRHKSHKRKSHKHKSHKHRSSHKSQAESSATINGNTGRQCHECKLYGATFMCSNCQNQWYCSRECQLSDWDTHHRTCGI

Target ORF information:

RefSeq Version	NM_140632.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RING-associated factor 2 (RAF2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140632.3

ORF Insert Sequence:

ATGTCCAACG AGATCTACGA CTCCTCCAAT TCGCAGCTAA GTAGCGACGA GTATAGCCAC 
GAGGAGGAGG AATACGACGC TCTAATGGAC GCGGCGGTGA GCAAGCAACG ACCCCAAAAG 
ACAAAATCCA AGGGATCCGA GTCCGAGAAA CCCGGAAATG GAGCTCCAGA ATCGGCGGCT 
GCCACTGAGA AAAATGAAAA GCCAGATGAC GATGCGGTGC CACCTTCCAC GCGCTCAGAA 
ACAATTCGGC GGATCCGGCA ATTGCAGTTA CAGCGTGGCA GGCTGCCCAA TCTTTACTTC 
AAGCGTCGCA GTATTCGTTG GCTGCGACCC AATAAAATGG ACTACAACAG CTATTTTGAC 
GGCCTGAAGG ACTTGCTGCC CAAGCGTAAA TTGAAGCACC TGGTTCGCTT CCATGACAGG 
CAGCGCAGAA CCTACGACAG CAGGAGCATC AGTCGTAGTC GCAGCCACAG CCGCAGTCTT 
AGCCGAAGTT GCAGTCGCAG TCGTAGTCGT AGCTATTCTC GCAGCCGCAG CGGCAGTGGT 
TCGCCGGAAC TCATTTGCCT TGACGACACG GAGAACGAGG ATTCGCCTGA GAAACCAGAG 
CAAGAGCCGG CAAGGGTTAC GCCCATCAAG GAAAATAAGC CTACGCTTCC GGCTCCACCC 
CAACTAAACT ACGAGCTGCC AGAAAGGAAG AAAGCAAACC CACTTGGAAA CAACGAAAAC 
GGTGCCTCTA TCCTCGACGA GTTCCTGGTA AAGAAGGATC CCCAGGAACC GGTCTTCGAC 
TACGGTAAAC TCTCAGCGTG TCGGGAATTG GAACAGGCTC TAAAGGACCG GTCGCGAGAT 
GCGCCAAATG AGGCAGAGTC GCTTGTGGTT GATGCCACTC TTAAAAGACG CCGTTCGGTA 
ACACCGCCCG CTAGCGAAGA GAAGCGCAAC AATGAAGTCG AGGATGATAA TATAATTGGG 
TTTTCAACGG TGCAGCAACA GAAGCCAATG GAGGCAGCTC CACCATTAAA TCTAAGAATG 
CCCACCACTC CATCGACTAA TCCTCTTCAG CGTCAGCAGG CTTTTAAGCT GAGCACACCG 
TATACCCTGG CTCCGCCTAC TGCCGCCAAG CAAGCTGACA CTTACAGTTT AAATACTCCA 
CCGCCAACGG CCACCCAAAT GCAGATATCC TATCCCAACG AAAAGCTGTC AAATAACATG 
AAAGCTAATG CGACTTTCTC GATTCAACAG ACAGCTTTAT CCCAAGTCGC TGCATCCTAT 
GCTGCTGCAC CACAAGTGGC TGCCCTCGCG CAGAGAGCTC CATCTCAGGT TGCTCCACTA 
CAACAAGCCC CCGTGCAACA GATGCACTTC CCAAGATCAG TTCCACCGCA GCAGCTTCCT 
CCACCACAGC AGCGAGCTCC TCCACAGCAA CTGGCTACAG CTCCATTCGC TCCACCACGG 
CACCTGGCTC AACATAGACC ATCTCCACAG CAGCTAGCTC CATCACAGCA GCTGGCTCCA 
CCACACCAGC TAGCTCCACC ACACCAGCTA GCTCCACCAC AGCAGCGAGC TCCGCCACAG 
CAGCTAGCTC CACCACACCA GCTAGCTCCA CCACACCAGC TAGCTCCACC ACAGCAGCGA 
GCTCCGCCAC AGCAGCTAGC TCCGCCACAG CAGCTAGCTC CACCACAGCA GCTAGCTCCA 
CCACAGCAGC TAGCTCCACC ACAGCAGCTA GCTCCACCAC AGCAGCGAGC TCCGCCACAG 
CAGCGAGCTC CGCCACAGCA GCTGGCTCCG CCACAACAGC GAGCTCCCAC ACAGCATATG 
GCTCCACCAC AACATATGGC TCCGCCACAG CAGCTAGCTC CAACACAGCA GCTAGCTCCT 
CCACCACAGC AGCTAGCGCC TCGACCACAG CAGCTAGCTC CTCCACCACA GCAACTAGCT 
CCTCCACCAC AGCAGCTAGC TCCTCCACCA CAGCAGCCAG ATTCATCAAG ACCAGCTTCG 
GTGCAGCCGT TGACCCCTCA AAGGCATGCT AGTCCACATC AGCTTGCCCA ATCACAGCAG 
GTCCATCAAT CGCCATCTCA ACTGCATCCT GTTCCAGTGG CTGCTCCCAA CTTTGCGGTG 
CCCCAACAGC CGCAACCCCG TCGATCGGAA ACGGTGAGCA CCCAGACGCA GGGCTTGGGG 
TCACCAGCGG CTTCTTCTTC GACAACCCGC CAAAAGTCAT TCGTTGATCT CAATAATAGT 
GATGCAAACT TCCATTATCG CGTCAAAGAA CTTTTCGATG AATTGAACTC GACAATGATT 
GATAAAATTA ACTCGGTACC CCCTGATGAA GATTCATTGA AAAAAGAGCG CGAACGCATC 
GTTGCGGATC TTGCAGCCCT AGATAAGCTG ATGGCCCAGA AAGAGGATGA ATATAACCGA 
CTGCTGTATT TGAGCTGCAT TAAGAAGGAG CTGCATGAAC GAATGGAGCG AAAGGAGCGC 
TTGATACGTA TTAAGGACCT ACTGCCCATC CTTATCAACA AGTGCGGCAC CAAGGAGCTC 
AGTGAGGTAA AGGCTATGCT AGAAGAGGAA ATCGATTCTC CAATGGCCTC CAAACATAGT 
TTGTCGGCAA TTGAAAAATT ACTGAATTAC GCCGAGTTAA ACCAGAACAT CATACAGCTT 
CTGCGGGGTT CCTTGGGCTT AAATAAACGC GCACCGTCCG TGTCTCCGGC CAATTTCGAG 
GATGACCAAA TGCTTTTTCG TAGAGATTCC TTGCCCGTAA GTCATCTATC AATGCGGGAT 
CAGCATTACG AATCTCGGAA TGCGGTAAAT GACCATCTGC TAGATAGCAA TCCAGACAGA 
GATCCGCCAG CCAAGCGACC GAAGCTAATC CACTCGCAAG ACAGTAACCA GGACATGGCA 
AGCTCCTCGA CACATTTGTC AGCCAATCTT TCAGAGACGC TTCGCAAGAT TAGGGCTTGG 
AAAAATTATT CGCATTTTAG TAACGTCTCA ATGGAGGACA ATAATTCGGA TAATGAATCA 
CAGATCGAAC CGCTGTTCAA CAGCAGTCCG ATGGCCTCCC GTCGCCAACT GGCCAATAGA 
CAGTCCTACC AAGAACAACA TTCCGCTAAT ATCAACGACC ATGAGAATGT CCAAAAGAGG 
CACAAGTCTC ACAAGCGCAA GTCGCATAAG CACAAGTCTC ACAAGCATAG GAGCTCCCAT 
AAGTCCCAGG CTGAATCTTC CGCCACCATC AACGGTAACA CAGGTAGACA ATGCCACGAG 
TGCAAGCTCT ATGGAGCCAC CTTTATGTGC TCCAACTGTC AGAATCAATG GTACTGCAGT 
CGTGAATGCC AGCTGAGCGA TTGGGACACA CATCACCGAA CTTGTGGCAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAF2 ( NM_168664.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730163.1 Drosophila melanogaster RING-associated factor 2 (RAF2), transcript variant A, mRNA.

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CloneID	OFa18692
Clone ID Related Accession (Same CDS sequence)	NM_001275014.1 , NM_140632.3 , NM_168664.2
Accession Version	NM_168664.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RING-associated factor 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168664.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAACG AGATCTACGA CTCCTCCAAT TCGCAGCTAA GTAGCGACGA GTATAGCCAC 
GAGGAGGAGG AATACGACGC TCTAATGGAC GCGGCGGTGA GCAAGCAACG ACCCCAAAAG 
ACAAAATCCA AGGGATCCGA GTCCGAGAAA CCCGGAAATG GAGCTCCAGA ATCGGCGGCT 
GCCACTGAGA AAAATGAAAA GCCAGATGAC GATGCGGTGC CACCTTCCAC GCGCTCAGAA 
ACAATTCGGC GGATCCGGCA ATTGCAGTTA CAGCGTGGCA GGCTGCCCAA TCTTTACTTC 
AAGCGTCGCA GTATTCGTTG GCTGCGACCC AATAAAATGG ACTACAACAG CTATTTTGAC 
GGCCTGAAGG ACTTGCTGCC CAAGCGTAAA TTGAAGCACC TGGTTCGCTT CCATGACAGG 
CAGCGCAGAA CCTACGACAG CAGGAGCATC AGTCGTAGTC GCAGCCACAG CCGCAGTCTT 
AGCCGAAGTT GCAGTCGCAG TCGTAGTCGT AGCTATTCTC GCAGCCGCAG CGGCAGTGGT 
TCGCCGGAAC TCATTTGCCT TGACGACACG GAGAACGAGG ATTCGCCTGA GAAACCAGAG 
CAAGAGCCGG CAAGGGTTAC GCCCATCAAG GAAAATAAGC CTACGCTTCC GGCTCCACCC 
CAACTAAACT ACGAGCTGCC AGAAAGGAAG AAAGCAAACC CACTTGGAAA CAACGAAAAC 
GGTGCCTCTA TCCTCGACGA GTTCCTGGTA AAGAAGGATC CCCAGGAACC GGTCTTCGAC 
TACGGTAAAC TCTCAGCGTG TCGGGAATTG GAACAGGCTC TAAAGGACCG GTCGCGAGAT 
GCGCCAAATG AGGCAGAGTC GCTTGTGGTT GATGCCACTC TTAAAAGACG CCGTTCGGTA 
ACACCGCCCG CTAGCGAAGA GAAGCGCAAC AATGAAGTCG AGGATGATAA TATAATTGGG 
TTTTCAACGG TGCAGCAACA GAAGCCAATG GAGGCAGCTC CACCATTAAA TCTAAGAATG 
CCCACCACTC CATCGACTAA TCCTCTTCAG CGTCAGCAGG CTTTTAAGCT GAGCACACCG 
TATACCCTGG CTCCGCCTAC TGCCGCCAAG CAAGCTGACA CTTACAGTTT AAATACTCCA 
CCGCCAACGG CCACCCAAAT GCAGATATCC TATCCCAACG AAAAGCTGTC AAATAACATG 
AAAGCTAATG CGACTTTCTC GATTCAACAG ACAGCTTTAT CCCAAGTCGC TGCATCCTAT 
GCTGCTGCAC CACAAGTGGC TGCCCTCGCG CAGAGAGCTC CATCTCAGGT TGCTCCACTA 
CAACAAGCCC CCGTGCAACA GATGCACTTC CCAAGATCAG TTCCACCGCA GCAGCTTCCT 
CCACCACAGC AGCGAGCTCC TCCACAGCAA CTGGCTACAG CTCCATTCGC TCCACCACGG 
CACCTGGCTC AACATAGACC ATCTCCACAG CAGCTAGCTC CATCACAGCA GCTGGCTCCA 
CCACACCAGC TAGCTCCACC ACACCAGCTA GCTCCACCAC AGCAGCGAGC TCCGCCACAG 
CAGCTAGCTC CACCACACCA GCTAGCTCCA CCACACCAGC TAGCTCCACC ACAGCAGCGA 
GCTCCGCCAC AGCAGCTAGC TCCGCCACAG CAGCTAGCTC CACCACAGCA GCTAGCTCCA 
CCACAGCAGC TAGCTCCACC ACAGCAGCTA GCTCCACCAC AGCAGCGAGC TCCGCCACAG 
CAGCGAGCTC CGCCACAGCA GCTGGCTCCG CCACAACAGC GAGCTCCCAC ACAGCATATG 
GCTCCACCAC AACATATGGC TCCGCCACAG CAGCTAGCTC CAACACAGCA GCTAGCTCCT 
CCACCACAGC AGCTAGCGCC TCGACCACAG CAGCTAGCTC CTCCACCACA GCAACTAGCT 
CCTCCACCAC AGCAGCTAGC TCCTCCACCA CAGCAGCCAG ATTCATCAAG ACCAGCTTCG 
GTGCAGCCGT TGACCCCTCA AAGGCATGCT AGTCCACATC AGCTTGCCCA ATCACAGCAG 
GTCCATCAAT CGCCATCTCA ACTGCATCCT GTTCCAGTGG CTGCTCCCAA CTTTGCGGTG 
CCCCAACAGC CGCAACCCCG TCGATCGGAA ACGGTGAGCA CCCAGACGCA GGGCTTGGGG 
TCACCAGCGG CTTCTTCTTC GACAACCCGC CAAAAGTCAT TCGTTGATCT CAATAATAGT 
GATGCAAACT TCCATTATCG CGTCAAAGAA CTTTTCGATG AATTGAACTC GACAATGATT 
GATAAAATTA ACTCGGTACC CCCTGATGAA GATTCATTGA AAAAAGAGCG CGAACGCATC 
GTTGCGGATC TTGCAGCCCT AGATAAGCTG ATGGCCCAGA AAGAGGATGA ATATAACCGA 
CTGCTGTATT TGAGCTGCAT TAAGAAGGAG CTGCATGAAC GAATGGAGCG AAAGGAGCGC 
TTGATACGTA TTAAGGACCT ACTGCCCATC CTTATCAACA AGTGCGGCAC CAAGGAGCTC 
AGTGAGGTAA AGGCTATGCT AGAAGAGGAA ATCGATTCTC CAATGGCCTC CAAACATAGT 
TTGTCGGCAA TTGAAAAATT ACTGAATTAC GCCGAGTTAA ACCAGAACAT CATACAGCTT 
CTGCGGGGTT CCTTGGGCTT AAATAAACGC GCACCGTCCG TGTCTCCGGC CAATTTCGAG 
GATGACCAAA TGCTTTTTCG TAGAGATTCC TTGCCCGTAA GTCATCTATC AATGCGGGAT 
CAGCATTACG AATCTCGGAA TGCGGTAAAT GACCATCTGC TAGATAGCAA TCCAGACAGA 
GATCCGCCAG CCAAGCGACC GAAGCTAATC CACTCGCAAG ACAGTAACCA GGACATGGCA 
AGCTCCTCGA CACATTTGTC AGCCAATCTT TCAGAGACGC TTCGCAAGAT TAGGGCTTGG 
AAAAATTATT CGCATTTTAG TAACGTCTCA ATGGAGGACA ATAATTCGGA TAATGAATCA 
CAGATCGAAC CGCTGTTCAA CAGCAGTCCG ATGGCCTCCC GTCGCCAACT GGCCAATAGA 
CAGTCCTACC AAGAACAACA TTCCGCTAAT ATCAACGACC ATGAGAATGT CCAAAAGAGG 
CACAAGTCTC ACAAGCGCAA GTCGCATAAG CACAAGTCTC ACAAGCATAG GAGCTCCCAT 
AAGTCCCAGG CTGAATCTTC CGCCACCATC AACGGTAACA CAGGTAGACA ATGCCACGAG 
TGCAAGCTCT ATGGAGCCAC CTTTATGTGC TCCAACTGTC AGAATCAATG GTACTGCAGT 
CGTGAATGCC AGCTGAGCGA TTGGGACACA CATCACCGAA CTTGTGGCAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730163.1
CDS	256..3609
Translation	MSNEIYDSSNSQLSSDEYSHEEEEYDALMDAAVSKQRPQKTKSKGSESEKPGNGAPESAAATEKNEKPDDDAVPPSTRSETIRRIRQLQLQRGRLPNLYFKRRSIRWLRPNKMDYNSYFDGLKDLLPKRKLKHLVRFHDRQRRTYDSRSISRSRSHSRSLSRSCSRSRSRSYSRSRSGSGSPELICLDDTENEDSPEKPEQEPARVTPIKENKPTLPAPPQLNYELPERKKANPLGNNENGASILDEFLVKKDPQEPVFDYGKLSACRELEQALKDRSRDAPNEAESLVVDATLKRRRSVTPPASEEKRNNEVEDDNIIGFSTVQQQKPMEAAPPLNLRMPTTPSTNPLQRQQAFKLSTPYTLAPPTAAKQADTYSLNTPPPTATQMQISYPNEKLSNNMKANATFSIQQTALSQVAASYAAAPQVAALAQRAPSQVAPLQQAPVQQMHFPRSVPPQQLPPPQQRAPPQQLATAPFAPPRHLAQHRPSPQQLAPSQQLAPPHQLAPPHQLAPPQQRAPPQQLAPPHQLAPPHQLAPPQQRAPPQQLAPPQQLAPPQQLAPPQQLAPPQQLAPPQQRAPPQQRAPPQQLAPPQQRAPTQHMAPPQHMAPPQQLAPTQQLAPPPQQLAPRPQQLAPPPQQLAPPPQQLAPPPQQPDSSRPASVQPLTPQRHASPHQLAQSQQVHQSPSQLHPVPVAAPNFAVPQQPQPRRSETVSTQTQGLGSPAASSSTTRQKSFVDLNNSDANFHYRVKELFDELNSTMIDKINSVPPDEDSLKKERERIVADLAALDKLMAQKEDEYNRLLYLSCIKKELHERMERKERLIRIKDLLPILINKCGTKELSEVKAMLEEEIDSPMASKHSLSAIEKLLNYAELNQNIIQLLRGSLGLNKRAPSVSPANFEDDQMLFRRDSLPVSHLSMRDQHYESRNAVNDHLLDSNPDRDPPAKRPKLIHSQDSNQDMASSSTHLSANLSETLRKIRAWKNYSHFSNVSMEDNNSDNESQIEPLFNSSPMASRRQLANRQSYQEQHSANINDHENVQKRHKSHKRKSHKHKSHKHRSSHKSQAESSATINGNTGRQCHECKLYGATFMCSNCQNQWYCSRECQLSDWDTHHRTCGI

Target ORF information:

RefSeq Version	NM_168664.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RING-associated factor 2 (RAF2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168664.2

ORF Insert Sequence:

ATGTCCAACG AGATCTACGA CTCCTCCAAT TCGCAGCTAA GTAGCGACGA GTATAGCCAC 
GAGGAGGAGG AATACGACGC TCTAATGGAC GCGGCGGTGA GCAAGCAACG ACCCCAAAAG 
ACAAAATCCA AGGGATCCGA GTCCGAGAAA CCCGGAAATG GAGCTCCAGA ATCGGCGGCT 
GCCACTGAGA AAAATGAAAA GCCAGATGAC GATGCGGTGC CACCTTCCAC GCGCTCAGAA 
ACAATTCGGC GGATCCGGCA ATTGCAGTTA CAGCGTGGCA GGCTGCCCAA TCTTTACTTC 
AAGCGTCGCA GTATTCGTTG GCTGCGACCC AATAAAATGG ACTACAACAG CTATTTTGAC 
GGCCTGAAGG ACTTGCTGCC CAAGCGTAAA TTGAAGCACC TGGTTCGCTT CCATGACAGG 
CAGCGCAGAA CCTACGACAG CAGGAGCATC AGTCGTAGTC GCAGCCACAG CCGCAGTCTT 
AGCCGAAGTT GCAGTCGCAG TCGTAGTCGT AGCTATTCTC GCAGCCGCAG CGGCAGTGGT 
TCGCCGGAAC TCATTTGCCT TGACGACACG GAGAACGAGG ATTCGCCTGA GAAACCAGAG 
CAAGAGCCGG CAAGGGTTAC GCCCATCAAG GAAAATAAGC CTACGCTTCC GGCTCCACCC 
CAACTAAACT ACGAGCTGCC AGAAAGGAAG AAAGCAAACC CACTTGGAAA CAACGAAAAC 
GGTGCCTCTA TCCTCGACGA GTTCCTGGTA AAGAAGGATC CCCAGGAACC GGTCTTCGAC 
TACGGTAAAC TCTCAGCGTG TCGGGAATTG GAACAGGCTC TAAAGGACCG GTCGCGAGAT 
GCGCCAAATG AGGCAGAGTC GCTTGTGGTT GATGCCACTC TTAAAAGACG CCGTTCGGTA 
ACACCGCCCG CTAGCGAAGA GAAGCGCAAC AATGAAGTCG AGGATGATAA TATAATTGGG 
TTTTCAACGG TGCAGCAACA GAAGCCAATG GAGGCAGCTC CACCATTAAA TCTAAGAATG 
CCCACCACTC CATCGACTAA TCCTCTTCAG CGTCAGCAGG CTTTTAAGCT GAGCACACCG 
TATACCCTGG CTCCGCCTAC TGCCGCCAAG CAAGCTGACA CTTACAGTTT AAATACTCCA 
CCGCCAACGG CCACCCAAAT GCAGATATCC TATCCCAACG AAAAGCTGTC AAATAACATG 
AAAGCTAATG CGACTTTCTC GATTCAACAG ACAGCTTTAT CCCAAGTCGC TGCATCCTAT 
GCTGCTGCAC CACAAGTGGC TGCCCTCGCG CAGAGAGCTC CATCTCAGGT TGCTCCACTA 
CAACAAGCCC CCGTGCAACA GATGCACTTC CCAAGATCAG TTCCACCGCA GCAGCTTCCT 
CCACCACAGC AGCGAGCTCC TCCACAGCAA CTGGCTACAG CTCCATTCGC TCCACCACGG 
CACCTGGCTC AACATAGACC ATCTCCACAG CAGCTAGCTC CATCACAGCA GCTGGCTCCA 
CCACACCAGC TAGCTCCACC ACACCAGCTA GCTCCACCAC AGCAGCGAGC TCCGCCACAG 
CAGCTAGCTC CACCACACCA GCTAGCTCCA CCACACCAGC TAGCTCCACC ACAGCAGCGA 
GCTCCGCCAC AGCAGCTAGC TCCGCCACAG CAGCTAGCTC CACCACAGCA GCTAGCTCCA 
CCACAGCAGC TAGCTCCACC ACAGCAGCTA GCTCCACCAC AGCAGCGAGC TCCGCCACAG 
CAGCGAGCTC CGCCACAGCA GCTGGCTCCG CCACAACAGC GAGCTCCCAC ACAGCATATG 
GCTCCACCAC AACATATGGC TCCGCCACAG CAGCTAGCTC CAACACAGCA GCTAGCTCCT 
CCACCACAGC AGCTAGCGCC TCGACCACAG CAGCTAGCTC CTCCACCACA GCAACTAGCT 
CCTCCACCAC AGCAGCTAGC TCCTCCACCA CAGCAGCCAG ATTCATCAAG ACCAGCTTCG 
GTGCAGCCGT TGACCCCTCA AAGGCATGCT AGTCCACATC AGCTTGCCCA ATCACAGCAG 
GTCCATCAAT CGCCATCTCA ACTGCATCCT GTTCCAGTGG CTGCTCCCAA CTTTGCGGTG 
CCCCAACAGC CGCAACCCCG TCGATCGGAA ACGGTGAGCA CCCAGACGCA GGGCTTGGGG 
TCACCAGCGG CTTCTTCTTC GACAACCCGC CAAAAGTCAT TCGTTGATCT CAATAATAGT 
GATGCAAACT TCCATTATCG CGTCAAAGAA CTTTTCGATG AATTGAACTC GACAATGATT 
GATAAAATTA ACTCGGTACC CCCTGATGAA GATTCATTGA AAAAAGAGCG CGAACGCATC 
GTTGCGGATC TTGCAGCCCT AGATAAGCTG ATGGCCCAGA AAGAGGATGA ATATAACCGA 
CTGCTGTATT TGAGCTGCAT TAAGAAGGAG CTGCATGAAC GAATGGAGCG AAAGGAGCGC 
TTGATACGTA TTAAGGACCT ACTGCCCATC CTTATCAACA AGTGCGGCAC CAAGGAGCTC 
AGTGAGGTAA AGGCTATGCT AGAAGAGGAA ATCGATTCTC CAATGGCCTC CAAACATAGT 
TTGTCGGCAA TTGAAAAATT ACTGAATTAC GCCGAGTTAA ACCAGAACAT CATACAGCTT 
CTGCGGGGTT CCTTGGGCTT AAATAAACGC GCACCGTCCG TGTCTCCGGC CAATTTCGAG 
GATGACCAAA TGCTTTTTCG TAGAGATTCC TTGCCCGTAA GTCATCTATC AATGCGGGAT 
CAGCATTACG AATCTCGGAA TGCGGTAAAT GACCATCTGC TAGATAGCAA TCCAGACAGA 
GATCCGCCAG CCAAGCGACC GAAGCTAATC CACTCGCAAG ACAGTAACCA GGACATGGCA 
AGCTCCTCGA CACATTTGTC AGCCAATCTT TCAGAGACGC TTCGCAAGAT TAGGGCTTGG 
AAAAATTATT CGCATTTTAG TAACGTCTCA ATGGAGGACA ATAATTCGGA TAATGAATCA 
CAGATCGAAC CGCTGTTCAA CAGCAGTCCG ATGGCCTCCC GTCGCCAACT GGCCAATAGA 
CAGTCCTACC AAGAACAACA TTCCGCTAAT ATCAACGACC ATGAGAATGT CCAAAAGAGG 
CACAAGTCTC ACAAGCGCAA GTCGCATAAG CACAAGTCTC ACAAGCATAG GAGCTCCCAT 
AAGTCCCAGG CTGAATCTTC CGCCACCATC AACGGTAACA CAGGTAGACA ATGCCACGAG 
TGCAAGCTCT ATGGAGCCAC CTTTATGTGC TCCAACTGTC AGAATCAATG GTACTGCAGT 
CGTGAATGCC AGCTGAGCGA TTGGGACACA CATCACCGAA CTTGTGGCAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RAF2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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