CG5235 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG5235
Entrez Gene ID	39758
Full Name	CG5235 gene product from transcript CG5235-RA
Synonyms	Dmel\CG5235
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 72D4-72D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_140576.4	NP_648833.1	uncharacterized protein, isoform A
NM_001274992.2	NP_001261921.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011450	XP_317853.3
Drosophila melanogaster (fruit fly)	CG5235	NP_648833.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG5235 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG5235 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18629	NM_140576.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5235), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa18629	NM_001274992.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5235), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG5235 ( NM_140576.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648833.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG5235), mRNA.

Price & Availability↑

CloneID	OFa18629
Clone ID Related Accession (Same CDS sequence)	NM_140576.4 , NM_001274992.2
Accession Version	NM_140576.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140576.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGTTC AAGACGTACT ATGGATCGTA CTCACGGTGC AGTTGAGTTT TGGTCTTGCG 
TATTTTGAAA ACAATCAAAG GACGCAGGAC TATGATGTCG ATAAAAATGG TTCTGTACAC 
CACAAAAACT GGGTGCGTAA CGAAATGATG GACAGCTTGG GCTTGTACTG GCTCAAATGG 
TGGATAAACG CCAACGAAAA TAGCATTTAC TTTGAAGTAA CTGTTCACAC ACGAGGCTTT 
GCCGGCCTGG GTTTCTCCAA GGATGGCCGG CTGGCCAGAG CGGATATGGT TTTGATGTGG 
GTGGATGACG CCACAGGACA TCCGAACGTC TTGGACTGCC ATGGGGCGCT GCATCCTTCA 
AGCGGACCGC CGCTGCAGGA CGATACTCAA AACTACGATG TCCTAGATGG CAAACAAAAT 
GGGACGCACA CAATCCTGAA GTTTAAACGC AAAATTGAGA CCTGTGACCC ATTCGATATT 
CCACTCTCCG CTGATACGTT TAAGGTACTG TGGTCCATTG GGGAAAATGA TCCTATTCAT 
GGAAACTTGG ATTGGCAAGG TCAGAGCAGA GGCGTGAAAG CTTTGCAATT GTTTTCGCCA 
ATGTTTACGA AAAATAGTCA CTCGATCGAA GGCATCCAAA AATGGGACAT AACTGTGAAC 
AACGTAACAA TTGATCGGTC AATGGATACA CTATACTGGT GTAAAATAGT GCGACTACCA 
GAGTTAACTG GCAAACAGCA TATAATTGGT TATGAGCCGC TGCTGTCTGG TAAATACGAG 
AGGAATGTTG TTCACCACAT GACCCTGTTT GAGTGTCAGT CCAAGATATA CTCTGGATCC 
GATCCTTCGT CCTGGGACCT TTGGGTTAGG AGTGCCGGCA CAGTGTGCAA CAGCAACCTA 
CTGACGCCAC GCGACTGGGA TTCGTGCTCT ACGCCAGTTG CAGTTTGGTC CTTGGGATCT 
GATGGACAGT TTCTACCTCC CCACGCAGGG ATCCCAATGG GCGGAGCATC TGGAGTTAGC 
TATTATATGC TGGAAATACA CTACGATAAT CCCGATGGAA AGGAATCGGT GGATCACTCC 
GGTTTTCGAA TACACTACAC ACCCAATCTG CGAACCTACG ATTCCGGAAT TCTAATAAGT 
GGTGTTTCCA TTTCGGAAAC GCAACTCATT CCGCCTGGTC AAAAGAAGTA TCGATCCGTC 
GGCATTTGTG GGCCGTCTTG TTCAAGCGTC ATGTTCCCAA AGGATGGTAT TAAAATAATA 
TCCGGAACGT TGCACTCACA TCAAGCTGGT CGCACAATTA GTCTTCGACA TGTTCGATCT 
GGTAAGGAGT TGAATCCGAT CATTGTTGAC GAAAACTACG ATTACAGGCA CCAAAAAGTC 
CATCAGCTTG CCAATGAAAC GGTCGTATTG CCAGGGGATT ACCTAATTAC AGACTGTTCC 
TATGAGACAA AGTACAGAAA ACGACCCACA TTCGGGGGCT ATTCCACGAA GGAGGAAATG 
TGTCTCACCT TTATTACTTA TTACCCAAAG ATTGAGATGT CCGGATGTTA TAGTATGACA 
CCAGTGCGTG AGTTTTTCGA GATGTTCCGT GTGTACCAAT TCTATTCCTT AAATATGACC 
GATGTCGAGA ACATGTTTCT CTACAACAGT GACTACACCG ACTACAGTAA ACAGGCGAAG 
AATGCGACAA ACAAACCAAA TTCCGGAAAA ACGTCCAAAG AAGACGTCAT CTACCAGGAG 
TCTTTGTTAA ACAAGCTTGT TATATCCGAC CCAGCCGAAT TTCACGATCG CACATTTCTA 
TCGCATTTAA ATCAGTTGCC TTGGCATGAT CCTCTCTTTA CGAAACGTGT TGAACAAGCA 
TTTATAACCG GAACACATAT GACGTTTTGT CGCGTATCCA AGGATTCATT ATCAATTCCT 
TCGGAAATAA TTCGCTATCC CGAGTTTACA GCGTACGTGA AGCCACCGGC AGCTTGTTTG 
AATTATTTGT TCACTGATAA TGAAGAACTT CGTTCGGGAA GTTCACAATT TTTTACAGAC 
TTTACGTTAA GTCTTTTATT ACTCATTCAG CTCGGGTTGC AAACGACATT ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648833.1
CDS	141..2237
Translation	MSVQDVLWIVLTVQLSFGLAYFENNQRTQDYDVDKNGSVHHKNWVRNEMMDSLGLYWLKWWINANENSIYFEVTVHTRGFAGLGFSKDGRLARADMVLMWVDDATGHPNVLDCHGALHPSSGPPLQDDTQNYDVLDGKQNGTHTILKFKRKIETCDPFDIPLSADTFKVLWSIGENDPIHGNLDWQGQSRGVKALQLFSPMFTKNSHSIEGIQKWDITVNNVTIDRSMDTLYWCKIVRLPELTGKQHIIGYEPLLSGKYERNVVHHMTLFECQSKIYSGSDPSSWDLWVRSAGTVCNSNLLTPRDWDSCSTPVAVWSLGSDGQFLPPHAGIPMGGASGVSYYMLEIHYDNPDGKESVDHSGFRIHYTPNLRTYDSGILISGVSISETQLIPPGQKKYRSVGICGPSCSSVMFPKDGIKIISGTLHSHQAGRTISLRHVRSGKELNPIIVDENYDYRHQKVHQLANETVVLPGDYLITDCSYETKYRKRPTFGGYSTKEEMCLTFITYYPKIEMSGCYSMTPVREFFEMFRVYQFYSLNMTDVENMFLYNSDYTDYSKQAKNATNKPNSGKTSKEDVIYQESLLNKLVISDPAEFHDRTFLSHLNQLPWHDPLFTKRVEQAFITGTHMTFCRVSKDSLSIPSEIIRYPEFTAYVKPPAACLNYLFTDNEELRSGSSQFFTDFTLSLLLLIQLGLQTTLL

Target ORF information:

RefSeq Version	NM_140576.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5235), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140576.4

ORF Insert Sequence:

ATGTCCGTTC AAGACGTACT ATGGATCGTA CTCACGGTGC AGTTGAGTTT TGGTCTTGCG 
TATTTTGAAA ACAATCAAAG GACGCAGGAC TATGATGTCG ATAAAAATGG TTCTGTACAC 
CACAAAAACT GGGTGCGTAA CGAAATGATG GACAGCTTGG GCTTGTACTG GCTCAAATGG 
TGGATAAACG CCAACGAAAA TAGCATTTAC TTTGAAGTAA CTGTTCACAC ACGAGGCTTT 
GCCGGCCTGG GTTTCTCCAA GGATGGCCGG CTGGCCAGAG CGGATATGGT TTTGATGTGG 
GTGGATGACG CCACAGGACA TCCGAACGTC TTGGACTGCC ATGGGGCGCT GCATCCTTCA 
AGCGGACCGC CGCTGCAGGA CGATACTCAA AACTACGATG TCCTAGATGG CAAACAAAAT 
GGGACGCACA CAATCCTGAA GTTTAAACGC AAAATTGAGA CCTGTGACCC ATTCGATATT 
CCACTCTCCG CTGATACGTT TAAGGTACTG TGGTCCATTG GGGAAAATGA TCCTATTCAT 
GGAAACTTGG ATTGGCAAGG TCAGAGCAGA GGCGTGAAAG CTTTGCAATT GTTTTCGCCA 
ATGTTTACGA AAAATAGTCA CTCGATCGAA GGCATCCAAA AATGGGACAT AACTGTGAAC 
AACGTAACAA TTGATCGGTC AATGGATACA CTATACTGGT GTAAAATAGT GCGACTACCA 
GAGTTAACTG GCAAACAGCA TATAATTGGT TATGAGCCGC TGCTGTCTGG TAAATACGAG 
AGGAATGTTG TTCACCACAT GACCCTGTTT GAGTGTCAGT CCAAGATATA CTCTGGATCC 
GATCCTTCGT CCTGGGACCT TTGGGTTAGG AGTGCCGGCA CAGTGTGCAA CAGCAACCTA 
CTGACGCCAC GCGACTGGGA TTCGTGCTCT ACGCCAGTTG CAGTTTGGTC CTTGGGATCT 
GATGGACAGT TTCTACCTCC CCACGCAGGG ATCCCAATGG GCGGAGCATC TGGAGTTAGC 
TATTATATGC TGGAAATACA CTACGATAAT CCCGATGGAA AGGAATCGGT GGATCACTCC 
GGTTTTCGAA TACACTACAC ACCCAATCTG CGAACCTACG ATTCCGGAAT TCTAATAAGT 
GGTGTTTCCA TTTCGGAAAC GCAACTCATT CCGCCTGGTC AAAAGAAGTA TCGATCCGTC 
GGCATTTGTG GGCCGTCTTG TTCAAGCGTC ATGTTCCCAA AGGATGGTAT TAAAATAATA 
TCCGGAACGT TGCACTCACA TCAAGCTGGT CGCACAATTA GTCTTCGACA TGTTCGATCT 
GGTAAGGAGT TGAATCCGAT CATTGTTGAC GAAAACTACG ATTACAGGCA CCAAAAAGTC 
CATCAGCTTG CCAATGAAAC GGTCGTATTG CCAGGGGATT ACCTAATTAC AGACTGTTCC 
TATGAGACAA AGTACAGAAA ACGACCCACA TTCGGGGGCT ATTCCACGAA GGAGGAAATG 
TGTCTCACCT TTATTACTTA TTACCCAAAG ATTGAGATGT CCGGATGTTA TAGTATGACA 
CCAGTGCGTG AGTTTTTCGA GATGTTCCGT GTGTACCAAT TCTATTCCTT AAATATGACC 
GATGTCGAGA ACATGTTTCT CTACAACAGT GACTACACCG ACTACAGTAA ACAGGCGAAG 
AATGCGACAA ACAAACCAAA TTCCGGAAAA ACGTCCAAAG AAGACGTCAT CTACCAGGAG 
TCTTTGTTAA ACAAGCTTGT TATATCCGAC CCAGCCGAAT TTCACGATCG CACATTTCTA 
TCGCATTTAA ATCAGTTGCC TTGGCATGAT CCTCTCTTTA CGAAACGTGT TGAACAAGCA 
TTTATAACCG GAACACATAT GACGTTTTGT CGCGTATCCA AGGATTCATT ATCAATTCCT 
TCGGAAATAA TTCGCTATCC CGAGTTTACA GCGTACGTGA AGCCACCGGC AGCTTGTTTG 
AATTATTTGT TCACTGATAA TGAAGAACTT CGTTCGGGAA GTTCACAATT TTTTACAGAC 
TTTACGTTAA GTCTTTTATT ACTCATTCAG CTCGGGTTGC AAACGACATT ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG5235 ( NM_001274992.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261921.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG5235), mRNA.

Price & Availability↑

CloneID	OFa18629
Clone ID Related Accession (Same CDS sequence)	NM_140576.4 , NM_001274992.2
Accession Version	NM_001274992.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001274992.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGTTC AAGACGTACT ATGGATCGTA CTCACGGTGC AGTTGAGTTT TGGTCTTGCG 
TATTTTGAAA ACAATCAAAG GACGCAGGAC TATGATGTCG ATAAAAATGG TTCTGTACAC 
CACAAAAACT GGGTGCGTAA CGAAATGATG GACAGCTTGG GCTTGTACTG GCTCAAATGG 
TGGATAAACG CCAACGAAAA TAGCATTTAC TTTGAAGTAA CTGTTCACAC ACGAGGCTTT 
GCCGGCCTGG GTTTCTCCAA GGATGGCCGG CTGGCCAGAG CGGATATGGT TTTGATGTGG 
GTGGATGACG CCACAGGACA TCCGAACGTC TTGGACTGCC ATGGGGCGCT GCATCCTTCA 
AGCGGACCGC CGCTGCAGGA CGATACTCAA AACTACGATG TCCTAGATGG CAAACAAAAT 
GGGACGCACA CAATCCTGAA GTTTAAACGC AAAATTGAGA CCTGTGACCC ATTCGATATT 
CCACTCTCCG CTGATACGTT TAAGGTACTG TGGTCCATTG GGGAAAATGA TCCTATTCAT 
GGAAACTTGG ATTGGCAAGG TCAGAGCAGA GGCGTGAAAG CTTTGCAATT GTTTTCGCCA 
ATGTTTACGA AAAATAGTCA CTCGATCGAA GGCATCCAAA AATGGGACAT AACTGTGAAC 
AACGTAACAA TTGATCGGTC AATGGATACA CTATACTGGT GTAAAATAGT GCGACTACCA 
GAGTTAACTG GCAAACAGCA TATAATTGGT TATGAGCCGC TGCTGTCTGG TAAATACGAG 
AGGAATGTTG TTCACCACAT GACCCTGTTT GAGTGTCAGT CCAAGATATA CTCTGGATCC 
GATCCTTCGT CCTGGGACCT TTGGGTTAGG AGTGCCGGCA CAGTGTGCAA CAGCAACCTA 
CTGACGCCAC GCGACTGGGA TTCGTGCTCT ACGCCAGTTG CAGTTTGGTC CTTGGGATCT 
GATGGACAGT TTCTACCTCC CCACGCAGGG ATCCCAATGG GCGGAGCATC TGGAGTTAGC 
TATTATATGC TGGAAATACA CTACGATAAT CCCGATGGAA AGGAATCGGT GGATCACTCC 
GGTTTTCGAA TACACTACAC ACCCAATCTG CGAACCTACG ATTCCGGAAT TCTAATAAGT 
GGTGTTTCCA TTTCGGAAAC GCAACTCATT CCGCCTGGTC AAAAGAAGTA TCGATCCGTC 
GGCATTTGTG GGCCGTCTTG TTCAAGCGTC ATGTTCCCAA AGGATGGTAT TAAAATAATA 
TCCGGAACGT TGCACTCACA TCAAGCTGGT CGCACAATTA GTCTTCGACA TGTTCGATCT 
GGTAAGGAGT TGAATCCGAT CATTGTTGAC GAAAACTACG ATTACAGGCA CCAAAAAGTC 
CATCAGCTTG CCAATGAAAC GGTCGTATTG CCAGGGGATT ACCTAATTAC AGACTGTTCC 
TATGAGACAA AGTACAGAAA ACGACCCACA TTCGGGGGCT ATTCCACGAA GGAGGAAATG 
TGTCTCACCT TTATTACTTA TTACCCAAAG ATTGAGATGT CCGGATGTTA TAGTATGACA 
CCAGTGCGTG AGTTTTTCGA GATGTTCCGT GTGTACCAAT TCTATTCCTT AAATATGACC 
GATGTCGAGA ACATGTTTCT CTACAACAGT GACTACACCG ACTACAGTAA ACAGGCGAAG 
AATGCGACAA ACAAACCAAA TTCCGGAAAA ACGTCCAAAG AAGACGTCAT CTACCAGGAG 
TCTTTGTTAA ACAAGCTTGT TATATCCGAC CCAGCCGAAT TTCACGATCG CACATTTCTA 
TCGCATTTAA ATCAGTTGCC TTGGCATGAT CCTCTCTTTA CGAAACGTGT TGAACAAGCA 
TTTATAACCG GAACACATAT GACGTTTTGT CGCGTATCCA AGGATTCATT ATCAATTCCT 
TCGGAAATAA TTCGCTATCC CGAGTTTACA GCGTACGTGA AGCCACCGGC AGCTTGTTTG 
AATTATTTGT TCACTGATAA TGAAGAACTT CGTTCGGGAA GTTCACAATT TTTTACAGAC 
TTTACGTTAA GTCTTTTATT ACTCATTCAG CTCGGGTTGC AAACGACATT ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261921.1
CDS	141..2237
Translation	MSVQDVLWIVLTVQLSFGLAYFENNQRTQDYDVDKNGSVHHKNWVRNEMMDSLGLYWLKWWINANENSIYFEVTVHTRGFAGLGFSKDGRLARADMVLMWVDDATGHPNVLDCHGALHPSSGPPLQDDTQNYDVLDGKQNGTHTILKFKRKIETCDPFDIPLSADTFKVLWSIGENDPIHGNLDWQGQSRGVKALQLFSPMFTKNSHSIEGIQKWDITVNNVTIDRSMDTLYWCKIVRLPELTGKQHIIGYEPLLSGKYERNVVHHMTLFECQSKIYSGSDPSSWDLWVRSAGTVCNSNLLTPRDWDSCSTPVAVWSLGSDGQFLPPHAGIPMGGASGVSYYMLEIHYDNPDGKESVDHSGFRIHYTPNLRTYDSGILISGVSISETQLIPPGQKKYRSVGICGPSCSSVMFPKDGIKIISGTLHSHQAGRTISLRHVRSGKELNPIIVDENYDYRHQKVHQLANETVVLPGDYLITDCSYETKYRKRPTFGGYSTKEEMCLTFITYYPKIEMSGCYSMTPVREFFEMFRVYQFYSLNMTDVENMFLYNSDYTDYSKQAKNATNKPNSGKTSKEDVIYQESLLNKLVISDPAEFHDRTFLSHLNQLPWHDPLFTKRVEQAFITGTHMTFCRVSKDSLSIPSEIIRYPEFTAYVKPPAACLNYLFTDNEELRSGSSQFFTDFTLSLLLLIQLGLQTTLL

Target ORF information:

RefSeq Version	NM_001274992.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5235), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274992.2

ORF Insert Sequence:

ATGTCCGTTC AAGACGTACT ATGGATCGTA CTCACGGTGC AGTTGAGTTT TGGTCTTGCG 
TATTTTGAAA ACAATCAAAG GACGCAGGAC TATGATGTCG ATAAAAATGG TTCTGTACAC 
CACAAAAACT GGGTGCGTAA CGAAATGATG GACAGCTTGG GCTTGTACTG GCTCAAATGG 
TGGATAAACG CCAACGAAAA TAGCATTTAC TTTGAAGTAA CTGTTCACAC ACGAGGCTTT 
GCCGGCCTGG GTTTCTCCAA GGATGGCCGG CTGGCCAGAG CGGATATGGT TTTGATGTGG 
GTGGATGACG CCACAGGACA TCCGAACGTC TTGGACTGCC ATGGGGCGCT GCATCCTTCA 
AGCGGACCGC CGCTGCAGGA CGATACTCAA AACTACGATG TCCTAGATGG CAAACAAAAT 
GGGACGCACA CAATCCTGAA GTTTAAACGC AAAATTGAGA CCTGTGACCC ATTCGATATT 
CCACTCTCCG CTGATACGTT TAAGGTACTG TGGTCCATTG GGGAAAATGA TCCTATTCAT 
GGAAACTTGG ATTGGCAAGG TCAGAGCAGA GGCGTGAAAG CTTTGCAATT GTTTTCGCCA 
ATGTTTACGA AAAATAGTCA CTCGATCGAA GGCATCCAAA AATGGGACAT AACTGTGAAC 
AACGTAACAA TTGATCGGTC AATGGATACA CTATACTGGT GTAAAATAGT GCGACTACCA 
GAGTTAACTG GCAAACAGCA TATAATTGGT TATGAGCCGC TGCTGTCTGG TAAATACGAG 
AGGAATGTTG TTCACCACAT GACCCTGTTT GAGTGTCAGT CCAAGATATA CTCTGGATCC 
GATCCTTCGT CCTGGGACCT TTGGGTTAGG AGTGCCGGCA CAGTGTGCAA CAGCAACCTA 
CTGACGCCAC GCGACTGGGA TTCGTGCTCT ACGCCAGTTG CAGTTTGGTC CTTGGGATCT 
GATGGACAGT TTCTACCTCC CCACGCAGGG ATCCCAATGG GCGGAGCATC TGGAGTTAGC 
TATTATATGC TGGAAATACA CTACGATAAT CCCGATGGAA AGGAATCGGT GGATCACTCC 
GGTTTTCGAA TACACTACAC ACCCAATCTG CGAACCTACG ATTCCGGAAT TCTAATAAGT 
GGTGTTTCCA TTTCGGAAAC GCAACTCATT CCGCCTGGTC AAAAGAAGTA TCGATCCGTC 
GGCATTTGTG GGCCGTCTTG TTCAAGCGTC ATGTTCCCAA AGGATGGTAT TAAAATAATA 
TCCGGAACGT TGCACTCACA TCAAGCTGGT CGCACAATTA GTCTTCGACA TGTTCGATCT 
GGTAAGGAGT TGAATCCGAT CATTGTTGAC GAAAACTACG ATTACAGGCA CCAAAAAGTC 
CATCAGCTTG CCAATGAAAC GGTCGTATTG CCAGGGGATT ACCTAATTAC AGACTGTTCC 
TATGAGACAA AGTACAGAAA ACGACCCACA TTCGGGGGCT ATTCCACGAA GGAGGAAATG 
TGTCTCACCT TTATTACTTA TTACCCAAAG ATTGAGATGT CCGGATGTTA TAGTATGACA 
CCAGTGCGTG AGTTTTTCGA GATGTTCCGT GTGTACCAAT TCTATTCCTT AAATATGACC 
GATGTCGAGA ACATGTTTCT CTACAACAGT GACTACACCG ACTACAGTAA ACAGGCGAAG 
AATGCGACAA ACAAACCAAA TTCCGGAAAA ACGTCCAAAG AAGACGTCAT CTACCAGGAG 
TCTTTGTTAA ACAAGCTTGT TATATCCGAC CCAGCCGAAT TTCACGATCG CACATTTCTA 
TCGCATTTAA ATCAGTTGCC TTGGCATGAT CCTCTCTTTA CGAAACGTGT TGAACAAGCA 
TTTATAACCG GAACACATAT GACGTTTTGT CGCGTATCCA AGGATTCATT ATCAATTCCT 
TCGGAAATAA TTCGCTATCC CGAGTTTACA GCGTACGTGA AGCCACCGGC AGCTTGTTTG 
AATTATTTGT TCACTGATAA TGAAGAACTT CGTTCGGGAA GTTCACAATT TTTTACAGAC 
TTTACGTTAA GTCTTTTATT ACTCATTCAG CTCGGGTTGC AAACGACATT ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG5235 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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