pHCl-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following pHCl-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pHCl-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa20953 NM_001038937.2
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa18602 NM_001274967.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa18603 NM_001274969.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant N, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa18604 NM_001274966.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa18605 NM_001274965.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa18606 NM_001259853.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa18607 NM_001259852.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa18608 NM_001274970.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant O, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa18609 NM_001274971.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant P, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa18610 NM_001274968.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant M, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa18611 NM_001169979.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa20950 NM_001038934.4
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa20951 NM_001038936.4
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa20952 NM_001038935.3
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa22021 NM_001300146.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant Q, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa22022 NM_001300147.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant R, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa87770 NM_001370024.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant S, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OFa87771 NM_001370025.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant T, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OFa87772 NM_001370026.1
Latest version!
Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant U, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa20953
    Clone ID Related Accession (Same CDS sequence) NM_001038937.2
    Accession Version NM_001038937.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_001038937.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GTAAGTCATT GGGATCGCAA
    TTGCCAGTAA TCGAATGCCA ATCACTTCAT TTGCTTATAC TTTGTATTAG CCAAAGAAAT
    TACAACCGGG AAATGTTTTG CTCTTTAGTA ATCGTAGTGT TTTTGATGGT AAACTGGTAT
    TTACTTTGTA CAACATACAA AAACGGCAAA TTCCATACAA TTATTTTAGG TTTTTTCGTG
    CAAAACGGAC CTTCTCAGTT TAGTACTCCA TTCTTTGGCT TGGCTTTAAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001034026.1
    CDS519..2432
    Translation

    Target ORF information:

    RefSeq Version NM_001038937.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001038937.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GTAAGTCATT GGGATCGCAA
    TTGCCAGTAA TCGAATGCCA ATCACTTCAT TTGCTTATAC TTTGTATTAG CCAAAGAAAT
    TACAACCGGG AAATGTTTTG CTCTTTAGTA ATCGTAGTGT TTTTGATGGT AAACTGGTAT
    TTACTTTGTA CAACATACAA AAACGGCAAA TTCCATACAA TTATTTTAGG TTTTTTCGTG
    CAAAACGGAC CTTCTCAGTT TAGTACTCCA TTCTTTGGCT TGGCTTTAAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18602
    Clone ID Related Accession (Same CDS sequence) NM_001274967.1
    Accession Version NM_001274967.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGGCA CTCTGACGGT GAACGTGAAC
    GTGCTGCTCC TGAGCTTAGC ATCGCCCGAC GAAAGCAGTT TGAAATACGA GGTTGAATTT
    CTGCTGAACC AGCAATGGAA CGATCCGCGA CTGCAATATG GCAATAAGTC TCATTATGAC
    TTCTTAAATG CTCTGCATCA TCATGATAGC ATCTGGACGC CGGATACGTA TTTCATTATG
    CATGGCGATT TCAAGGATCC CATCATACCA ATGCATTTCG CTTTAAGAAT ATTTCGGAAC
    GGGACCATTA CGTACGCAAT GAGACGTCAC CTGATCTTAT CCTGCCAGGG AAGTCTTCAT
    ATATTTCCAT TCGATGACCC CAAGTGTTCC TTCTCCATGG AGAGCATTTC CTACGAGGAG
    GCCCAAATCA AGTACGTCTG GAAAAACGAT GAGGACACTC TGCGGAAGAG TCCTTCGCTG
    ACCACATTGA ACGCGTACTT AATCAAGAAT CAAACGACGG CCTGCGATCA GAACAGCTGG
    AGAGGTAATT ACAGTTGCCT ACAGGTCGAG TTGACATTTA CCCGTGATCG TGCGTATTAT
    TTTACAACCG TTTTCATACC TGGCATTATA TTGGTCACCT CGTCGTTTAT CACATTTTGG
    CTGGAATGGA ATGCAGTCCC AGCCCGGGTT ATGATCGGCG TGACAACAAT GCTGAATTTC
    TTCACTACCT CGAACGGTTT CCGGAGCACT CTGCCGGTTG TTTCTAATCT GACGGCGATG
    AACGTGTGGG ACGGCGTGTG TATGTGCTTC ATCTACGCCT CCTTGCTGGA ATTCGTCTGC
    GTCAATTATG TGGGGCGAAA GCGACCCCTG CACAACGTCG TCTATCGGCC CGGCGAGAAT
    CCCGTAACAC AGCGTCTTCC AGCAGTACTA AGCAGGATCG GAGTAATTCT TGCAAGTCCT
    TTGCCCGGCG AAAAGCGTCA AAACGCCGGG GCACCGAACG AAATTGTGGC ATGCACCACC
    TGCGGCGGCA GCAACAGTCC GTGCACCCAT TCGGCAAATA ATGGATGTGC CACGGAGACC
    TGCTTCGTTC AAGTGCGGAA AAAGGAGCCA CCTCATCCGA TTCGAGTGGC CAAGACGATC
    GATGTCATCG CTAGAATTAC ATTTCCAACT GCTTATGCCA TTTTTCTGAT ATTCTTCTTT
    GTACACTATA AAGGATTTTC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261896.1
    CDS524..2767
    Translation

    Target ORF information:

    RefSeq Version NM_001274967.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274967.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGGCA CTCTGACGGT GAACGTGAAC
    GTGCTGCTCC TGAGCTTAGC ATCGCCCGAC GAAAGCAGTT TGAAATACGA GGTTGAATTT
    CTGCTGAACC AGCAATGGAA CGATCCGCGA CTGCAATATG GCAATAAGTC TCATTATGAC
    TTCTTAAATG CTCTGCATCA TCATGATAGC ATCTGGACGC CGGATACGTA TTTCATTATG
    CATGGCGATT TCAAGGATCC CATCATACCA ATGCATTTCG CTTTAAGAAT ATTTCGGAAC
    GGGACCATTA CGTACGCAAT GAGACGTCAC CTGATCTTAT CCTGCCAGGG AAGTCTTCAT
    ATATTTCCAT TCGATGACCC CAAGTGTTCC TTCTCCATGG AGAGCATTTC CTACGAGGAG
    GCCCAAATCA AGTACGTCTG GAAAAACGAT GAGGACACTC TGCGGAAGAG TCCTTCGCTG
    ACCACATTGA ACGCGTACTT AATCAAGAAT CAAACGACGG CCTGCGATCA GAACAGCTGG
    AGAGGTAATT ACAGTTGCCT ACAGGTCGAG TTGACATTTA CCCGTGATCG TGCGTATTAT
    TTTACAACCG TTTTCATACC TGGCATTATA TTGGTCACCT CGTCGTTTAT CACATTTTGG
    CTGGAATGGA ATGCAGTCCC AGCCCGGGTT ATGATCGGCG TGACAACAAT GCTGAATTTC
    TTCACTACCT CGAACGGTTT CCGGAGCACT CTGCCGGTTG TTTCTAATCT GACGGCGATG
    AACGTGTGGG ACGGCGTGTG TATGTGCTTC ATCTACGCCT CCTTGCTGGA ATTCGTCTGC
    GTCAATTATG TGGGGCGAAA GCGACCCCTG CACAACGTCG TCTATCGGCC CGGCGAGAAT
    CCCGTAACAC AGCGTCTTCC AGCAGTACTA AGCAGGATCG GAGTAATTCT TGCAAGTCCT
    TTGCCCGGCG AAAAGCGTCA AAACGCCGGG GCACCGAACG AAATTGTGGC ATGCACCACC
    TGCGGCGGCA GCAACAGTCC GTGCACCCAT TCGGCAAATA ATGGATGTGC CACGGAGACC
    TGCTTCGTTC AAGTGCGGAA AAAGGAGCCA CCTCATCCGA TTCGAGTGGC CAAGACGATC
    GATGTCATCG CTAGAATTAC ATTTCCAACT GCTTATGCCA TTTTTCTGAT ATTCTTCTTT
    GTACACTATA AAGGATTTTC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18603
    Clone ID Related Accession (Same CDS sequence) NM_001274969.1
    Accession Version NM_001274969.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2937bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform N
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GTAATTACAG TTGCCTACAG
    GTCGAGTTGA CATTTACCCG TGATCGTGCG TATTATTTTA CAACCGTTTT CATACCTGGC
    ATTATATTGG TCACCTCGTC GTTTATCACA TTTTGGCTGG AATGGAATGC AGTCCCAGCC
    CGGGTTATGA TCGGCGTGAC AACAATGCTG AATTTCTTCA CTACCTCGAA CGGTTTCCGG
    AGCACTCTGC CGGTTGTTTC TAATCTGACG GCGATGAACG TGTGGGACGG CGTGTGTATG
    TGCTTCATCT ACGCCTCCTT GCTGGAATTC GTCTGCGTCA ATTATGTGGG GCGAAAGCGA
    CCCCTGCACA ACGTCGTCTA TCGGCCCGGC GAGAATCCCG TAACACAGCG TCTTCCAGCA
    GTACTAAGCA GGATCGGAGT AATTCTTGCA AGTCCTTTGG CAAGCGCCGC TGCCACGCCG
    GCCATGTCGA TGATGGGCGG GGCCACGCCC ATGTCCAGTG GTACCCCGGC CGCCTCATCC
    TCGGTGGCCA CGCCCGGTAC CCCAAGGATC ACCGATCCGG AGGAGTTCTT CGGCGGCGGC
    ATGCGTTGGC AGGAGGCCCA TGGCGGAATC ATTGGAGCTG CTGTCCAGAA TTTACGGGCA
    CGTTCGATAA AACGTAAGGC GGCCAGAAGT CCATCCACTT CCATATCGCC CCATCCCAGT
    GGGCGACCCA CTGAGCATAT CTACGAAGAG CCCGGTGGAT CCACATCCGG TGTCACAAGT
    ACGAAAGTAA AGTTCAAGGA CGAGCTGAAG GAGGAGCAAC CGGAGGAGGC GGAGTCCAGC
    ACCAAACTAC GACGATCGGT GGCCACCAGT ACACCGGCTT TGGTTGTAAG GATCGAGGCT
    GAGATCGATC CGGAGGCGGA AGACAAGCCC ATCCAGGATA TGGAAATGAC AGAACGTCAG
    TTGTCCTTGC CGTTGAAACC GCCGCGTCGC AAGGCATCGC GCTCCAATTC ACCAGCCTCG
    GTTTACAGCG ATGCATTCGA GAATGTGGAA GCTACACCCG GCGAAAAGCG TCAAAACGCC
    GGGGCACCGA ACGAAATTGT GGCATGCACC ACCTGCGGCG GCAGCAACAG TCCGTGCACC
    CATTCGGCAA ATAATGGATG TGCCACGGAG ACCTGCTTCG TTCAAGTGCG GAAAAAGGAG
    CCACCTCATC CGATTCGAGT GGCCAAGACG ATCGATGTCA TCGCTAGAAT TACATTTCCA
    ACTGCTTATG CCATTTTTCT GATATTCTTC TTTGTACACT ATAAAGGATT TTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261898.1
    CDS519..3455
    Translation

    Target ORF information:

    RefSeq Version NM_001274969.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant N, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274969.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GTAATTACAG TTGCCTACAG
    GTCGAGTTGA CATTTACCCG TGATCGTGCG TATTATTTTA CAACCGTTTT CATACCTGGC
    ATTATATTGG TCACCTCGTC GTTTATCACA TTTTGGCTGG AATGGAATGC AGTCCCAGCC
    CGGGTTATGA TCGGCGTGAC AACAATGCTG AATTTCTTCA CTACCTCGAA CGGTTTCCGG
    AGCACTCTGC CGGTTGTTTC TAATCTGACG GCGATGAACG TGTGGGACGG CGTGTGTATG
    TGCTTCATCT ACGCCTCCTT GCTGGAATTC GTCTGCGTCA ATTATGTGGG GCGAAAGCGA
    CCCCTGCACA ACGTCGTCTA TCGGCCCGGC GAGAATCCCG TAACACAGCG TCTTCCAGCA
    GTACTAAGCA GGATCGGAGT AATTCTTGCA AGTCCTTTGG CAAGCGCCGC TGCCACGCCG
    GCCATGTCGA TGATGGGCGG GGCCACGCCC ATGTCCAGTG GTACCCCGGC CGCCTCATCC
    TCGGTGGCCA CGCCCGGTAC CCCAAGGATC ACCGATCCGG AGGAGTTCTT CGGCGGCGGC
    ATGCGTTGGC AGGAGGCCCA TGGCGGAATC ATTGGAGCTG CTGTCCAGAA TTTACGGGCA
    CGTTCGATAA AACGTAAGGC GGCCAGAAGT CCATCCACTT CCATATCGCC CCATCCCAGT
    GGGCGACCCA CTGAGCATAT CTACGAAGAG CCCGGTGGAT CCACATCCGG TGTCACAAGT
    ACGAAAGTAA AGTTCAAGGA CGAGCTGAAG GAGGAGCAAC CGGAGGAGGC GGAGTCCAGC
    ACCAAACTAC GACGATCGGT GGCCACCAGT ACACCGGCTT TGGTTGTAAG GATCGAGGCT
    GAGATCGATC CGGAGGCGGA AGACAAGCCC ATCCAGGATA TGGAAATGAC AGAACGTCAG
    TTGTCCTTGC CGTTGAAACC GCCGCGTCGC AAGGCATCGC GCTCCAATTC ACCAGCCTCG
    GTTTACAGCG ATGCATTCGA GAATGTGGAA GCTACACCCG GCGAAAAGCG TCAAAACGCC
    GGGGCACCGA ACGAAATTGT GGCATGCACC ACCTGCGGCG GCAGCAACAG TCCGTGCACC
    CATTCGGCAA ATAATGGATG TGCCACGGAG ACCTGCTTCG TTCAAGTGCG GAAAAAGGAG
    CCACCTCATC CGATTCGAGT GGCCAAGACG ATCGATGTCA TCGCTAGAAT TACATTTCCA
    ACTGCTTATG CCATTTTTCT GATATTCTTC TTTGTACACT ATAAAGGATT TTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18604
    Clone ID Related Accession (Same CDS sequence) NM_001274966.1
    Accession Version NM_001274966.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGGCA CTCTGACGGT GAACGTGAAC
    GTGCTGCTCC TGAGCTTAGC ATCGCCCGAC GAAAGCAGTT TGAAATACGA GGTTGAATTT
    CTGCTGAACC AGCAATGGAA CGATCCGCGA CTGCAATATG GCAATAAGTC TCATTATGAC
    TTCTTAAATG CTCTGCATCA TCATGATAGC ATCTGGACGC CGGATACGTA TTTCATTATG
    CATGGCGATT TCAAGGATCC CATCATACCA ATGCATTTCG CTTTAAGAAT ATTTCGGAAC
    GGGACCATTA CGTACGCAAT GAGACGTCAC CTGATCTTAT CCTGCCAGGG AAGTCTTCAT
    ATATTTCCAT TCGATGACCC CAAGTGTTCC TTCTCCATGG AGAGCATTTC CTACGAGGAG
    GCCCAAATCA AGTACGTCTG GAAAAACGAT GAGGACACTC TGCGGAAGAG TCCTTCGCTG
    ACCACATTGA ACGCGTACTT AATCAAGAAT CAAACGACGG CCTGCGATCA GAACAGCTGG
    AGAGGGAACT ACAGCTGTCT TAGGGTCGAT TTAATCTTTA CCAGAGATCG TGCATTCTAT
    TTCACCACCG TTTTTATACC CGGCATTATA TTGGTGACGT CGTCCTTTAT AACTTTCTGG
    CTAGAATGGA ATGCCGTGCC AGCTAGATCG ATGATAGGCG TGACAACAAT GCTGAATTTC
    TTCACTACCT CGAACGGTTT CCGGAGCACT CTGCCGGTTG TTTCTAATCT GACGGCGATG
    AACGTGTGGG ACGGCGTGTG TATGTGCTTC ATCTACGCCT CCTTGCTGGA ATTCGTCTGC
    GTCAATTATG TGGGGCGAAA GCGACCCCTG CACAACGTCG TCTATCGGCC CGGCGAGAAT
    CCCGTAACAC AGCGTCTTCC AGCAGTACTA AGCAGGATCG GAGTAATTCT TGCAAGTCCT
    TTGGGCGAAA AGCGTCAAAA CGCCGGGGCA CCGAACGAAA TTGTGGCATG CACCACCTGC
    GGCGGCAGCA ACAGTCCGTG CACCCATTCG GCAAATAATG GATGTGCCAC GGAGACCTGC
    TTCGTTCAAG TGCGGAAAAA GGAGCCACCT CATCCGATTC GAGTGGCCAA GACGATCGAT
    GTCATCGCTA GAATTACATT TCCAACTGCT TATGCCATTT TTCTGATATT CTTCTTTGTA
    CACTATAAAG GATTTTCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261895.1
    CDS524..2764
    Translation

    Target ORF information:

    RefSeq Version NM_001274966.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274966.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGGCA CTCTGACGGT GAACGTGAAC
    GTGCTGCTCC TGAGCTTAGC ATCGCCCGAC GAAAGCAGTT TGAAATACGA GGTTGAATTT
    CTGCTGAACC AGCAATGGAA CGATCCGCGA CTGCAATATG GCAATAAGTC TCATTATGAC
    TTCTTAAATG CTCTGCATCA TCATGATAGC ATCTGGACGC CGGATACGTA TTTCATTATG
    CATGGCGATT TCAAGGATCC CATCATACCA ATGCATTTCG CTTTAAGAAT ATTTCGGAAC
    GGGACCATTA CGTACGCAAT GAGACGTCAC CTGATCTTAT CCTGCCAGGG AAGTCTTCAT
    ATATTTCCAT TCGATGACCC CAAGTGTTCC TTCTCCATGG AGAGCATTTC CTACGAGGAG
    GCCCAAATCA AGTACGTCTG GAAAAACGAT GAGGACACTC TGCGGAAGAG TCCTTCGCTG
    ACCACATTGA ACGCGTACTT AATCAAGAAT CAAACGACGG CCTGCGATCA GAACAGCTGG
    AGAGGGAACT ACAGCTGTCT TAGGGTCGAT TTAATCTTTA CCAGAGATCG TGCATTCTAT
    TTCACCACCG TTTTTATACC CGGCATTATA TTGGTGACGT CGTCCTTTAT AACTTTCTGG
    CTAGAATGGA ATGCCGTGCC AGCTAGATCG ATGATAGGCG TGACAACAAT GCTGAATTTC
    TTCACTACCT CGAACGGTTT CCGGAGCACT CTGCCGGTTG TTTCTAATCT GACGGCGATG
    AACGTGTGGG ACGGCGTGTG TATGTGCTTC ATCTACGCCT CCTTGCTGGA ATTCGTCTGC
    GTCAATTATG TGGGGCGAAA GCGACCCCTG CACAACGTCG TCTATCGGCC CGGCGAGAAT
    CCCGTAACAC AGCGTCTTCC AGCAGTACTA AGCAGGATCG GAGTAATTCT TGCAAGTCCT
    TTGGGCGAAA AGCGTCAAAA CGCCGGGGCA CCGAACGAAA TTGTGGCATG CACCACCTGC
    GGCGGCAGCA ACAGTCCGTG CACCCATTCG GCAAATAATG GATGTGCCAC GGAGACCTGC
    TTCGTTCAAG TGCGGAAAAA GGAGCCACCT CATCCGATTC GAGTGGCCAA GACGATCGAT
    GTCATCGCTA GAATTACATT TCCAACTGCT TATGCCATTT TTCTGATATT CTTCTTTGTA
    CACTATAAAG GATTTTCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18605
    Clone ID Related Accession (Same CDS sequence) NM_001274965.1
    Accession Version NM_001274965.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGATG AAGACTTTTG CTGTGGCTTG
    CAATCGCCCG ATATGGCAAC TAATCAAAAT GCACGGAGAC CACACAATCG CGGCACTCTG
    ACGGTGAACG TGAACGTGCT GCTCCTGAGC TTAGCATCGC CCGACGAAAG CAGTTTGAAA
    TACGAGGTTG AATTTCTGCT GAACCAGCAA TGGAACGATC CGCGACTGCA ATATGGCAAT
    AAGTCTCATT ATGACTTCTT AAATGCTCTG CATCATCATG ATAGCATCTG GACGCCGGAT
    ACGTATTTCA TTATGCATGG CGATTTCAAG GATCCCATCA TACCAATGCA TTTCGCTTTA
    AGAATATTTC GGAACGGGAC CATTACGTAC GCAATGAGAC GTCACCTGAT CTTATCCTGC
    CAGGGAAGTC TTCATATATT TCCATTCGAT GACCCCAAGT GTTCCTTCTC CATGGAGAGC
    ATTTCCTACG AGGAGGCCCA AATCAAGTAC GTCTGGAAAA ACGATGAGGA CACTCTGCGG
    AAGAGTCCTT CGCTGACCAC ATTGAACGCG TACTTAATCA AGAATCAAAC GACGGCCTGC
    GATCAGAACA GCTGGAGAGG TAATTACAGT TGCCTACAGG TCGAGTTGAC ATTTACCCGT
    GATCGTGCGT ATTATTTTAC AACCGTTTTC ATACCTGGCA TTATATTGGT CACCTCGTCG
    TTTATCACAT TTTGGCTGGA ATGGAATGCA GTCCCAGCCC GGGTTATGAT CGGCGTGACA
    ACAATGCTGA ATTTCTTCAC TACCTCGAAC GGTTTCCGGA GCACTCTGCC GGTTGTTTCT
    AATCTGACGG CGATGAACGT GTGGGACGGC GTGTGTATGT GCTTCATCTA CGCCTCCTTG
    CTGGAATTCG TCTGCGTCAA TTATGTGGGG CGAAAGCGAC CCCTGCACAA CGTCGTCTAT
    CGGCCCGGCG AGAATCCCGT AACACAGCGT CTTCCAGCAG TACTAAGCAG GATCGGAGTA
    ATTCTTGCAA GTCCTTTGCC CGGCGAAAAG CGTCAAAACG CCGGGGCACC GAACGAAATT
    GTGGCATGCA CCACCTGCGG CGGCAGCAAC AGTCCGTGCA CCCATTCGGC AAATAATGGA
    TGTGCCACGG AGACCTGCTT CGTTCAAGTG CGGAAAAAGG AGCCACCTCA TCCGATTCGA
    GTGGCCAAGA CGATCGATGT CATCGCTAGA ATTACATTTC CAACTGCTTA TGCCATTTTT
    CTGATATTCT TCTTTGTACA CTATAAAGGA TTTTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261894.1
    CDS524..2842
    Translation

    Target ORF information:

    RefSeq Version NM_001274965.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274965.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGATG AAGACTTTTG CTGTGGCTTG
    CAATCGCCCG ATATGGCAAC TAATCAAAAT GCACGGAGAC CACACAATCG CGGCACTCTG
    ACGGTGAACG TGAACGTGCT GCTCCTGAGC TTAGCATCGC CCGACGAAAG CAGTTTGAAA
    TACGAGGTTG AATTTCTGCT GAACCAGCAA TGGAACGATC CGCGACTGCA ATATGGCAAT
    AAGTCTCATT ATGACTTCTT AAATGCTCTG CATCATCATG ATAGCATCTG GACGCCGGAT
    ACGTATTTCA TTATGCATGG CGATTTCAAG GATCCCATCA TACCAATGCA TTTCGCTTTA
    AGAATATTTC GGAACGGGAC CATTACGTAC GCAATGAGAC GTCACCTGAT CTTATCCTGC
    CAGGGAAGTC TTCATATATT TCCATTCGAT GACCCCAAGT GTTCCTTCTC CATGGAGAGC
    ATTTCCTACG AGGAGGCCCA AATCAAGTAC GTCTGGAAAA ACGATGAGGA CACTCTGCGG
    AAGAGTCCTT CGCTGACCAC ATTGAACGCG TACTTAATCA AGAATCAAAC GACGGCCTGC
    GATCAGAACA GCTGGAGAGG TAATTACAGT TGCCTACAGG TCGAGTTGAC ATTTACCCGT
    GATCGTGCGT ATTATTTTAC AACCGTTTTC ATACCTGGCA TTATATTGGT CACCTCGTCG
    TTTATCACAT TTTGGCTGGA ATGGAATGCA GTCCCAGCCC GGGTTATGAT CGGCGTGACA
    ACAATGCTGA ATTTCTTCAC TACCTCGAAC GGTTTCCGGA GCACTCTGCC GGTTGTTTCT
    AATCTGACGG CGATGAACGT GTGGGACGGC GTGTGTATGT GCTTCATCTA CGCCTCCTTG
    CTGGAATTCG TCTGCGTCAA TTATGTGGGG CGAAAGCGAC CCCTGCACAA CGTCGTCTAT
    CGGCCCGGCG AGAATCCCGT AACACAGCGT CTTCCAGCAG TACTAAGCAG GATCGGAGTA
    ATTCTTGCAA GTCCTTTGCC CGGCGAAAAG CGTCAAAACG CCGGGGCACC GAACGAAATT
    GTGGCATGCA CCACCTGCGG CGGCAGCAAC AGTCCGTGCA CCCATTCGGC AAATAATGGA
    TGTGCCACGG AGACCTGCTT CGTTCAAGTG CGGAAAAAGG AGCCACCTCA TCCGATTCGA
    GTGGCCAAGA CGATCGATGT CATCGCTAGA ATTACATTTC CAACTGCTTA TGCCATTTTT
    CTGATATTCT TCTTTGTACA CTATAAAGGA TTTTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18606
    Clone ID Related Accession (Same CDS sequence) NM_001259853.1
    Accession Version NM_001259853.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGGC
    ACTCTGACGG TGAACGTGAA CGTGCTGCTC CTGAGCTTAG CATCGCCCGA CGAAAGCAGT
    TTGAAATACG AGGTTGAATT TCTGCTGAAC CAGCAATGGA ACGATCCGCG ACTGCAATAT
    GGCAATAAGT CTCATTATGA CTTCTTAAAT GCTCTGCATC ATCATGATAG CATCTGGACG
    CCGGATACGT ATTTCATTAT GCATGGCGAT TTCAAGGATC CCATCATACC AATGCATTTC
    GCTTTAAGAA TATTTCGGAA CGGGACCATT ACGTACGCAA TGAGACGTCA CCTGATCTTA
    TCCTGCCAGG GAAGTCTTCA TATATTTCCA TTCGATGACC CCAAGTGTTC CTTCTCCATG
    GAGAGCATTT CCTACGAGGA GGCCCAAATC AAGTACGTCT GGAAAAACGA TGAGGACACT
    CTGCGGAAGA GTCCTTCGCT GACCACATTG AACGCGTACT TAATCAAGAA TCAAACGACG
    GCCTGCGATC AGAACAGCTG GAGAGGGAAC TACAGCTGTC TTAGGGTCGA TTTAATCTTT
    ACCAGAGATC GTGCATTCTA TTTCACCACC GTTTTTATAC CCGGCATTAT ATTGGTGACG
    TCGTCCTTTA TAACTTTCTG GCTAGAATGG AATGCCGTGC CAGCTAGATC GATGATAGGC
    GTGACAACAA TGCTGAATTT CTTCACTACC TCGAACGGTT TCCGGAGCAC TCTGCCGGTT
    GTTTCTAATC TGACGGCGAT GAACGTGTGG GACGGCGTGT GTATGTGCTT CATCTACGCC
    TCCTTGCTGG AATTCGTCTG CGTCAATTAT GTGGGGCGAA AGCGACCCCT GCACAACGTC
    GTCTATCGGC CCGGCGAGAA TCCCGTAACA CAGCGTCTTC CAGCAGTACT AAGCAGGATC
    GGAGTAATTC TTGCAAGTCC TTTGCCCGGC GAAAAGCGTC AAAACGCCGG GGCACCGAAC
    GAAATTGTGG CATGCACCAC CTGCGGCGGC AGCAACAGTC CGTGCACCCA TTCGGCAAAT
    AATGGATGTG CCACGGAGAC CTGCTTCGTT CAAGTGCGGA AAAAGGAGCC ACCTCATCCG
    ATTCGAGTGG CCAAGACGAT CGATGTCATC GCTAGAATTA CATTTCCAAC TGCTTATGCC
    ATTTTTCTGA TATTCTTCTT TGTACACTAT AAAGGATTTT CGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246782.1
    CDS524..2788
    Translation

    Target ORF information:

    RefSeq Version NM_001259853.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGGC
    ACTCTGACGG TGAACGTGAA CGTGCTGCTC CTGAGCTTAG CATCGCCCGA CGAAAGCAGT
    TTGAAATACG AGGTTGAATT TCTGCTGAAC CAGCAATGGA ACGATCCGCG ACTGCAATAT
    GGCAATAAGT CTCATTATGA CTTCTTAAAT GCTCTGCATC ATCATGATAG CATCTGGACG
    CCGGATACGT ATTTCATTAT GCATGGCGAT TTCAAGGATC CCATCATACC AATGCATTTC
    GCTTTAAGAA TATTTCGGAA CGGGACCATT ACGTACGCAA TGAGACGTCA CCTGATCTTA
    TCCTGCCAGG GAAGTCTTCA TATATTTCCA TTCGATGACC CCAAGTGTTC CTTCTCCATG
    GAGAGCATTT CCTACGAGGA GGCCCAAATC AAGTACGTCT GGAAAAACGA TGAGGACACT
    CTGCGGAAGA GTCCTTCGCT GACCACATTG AACGCGTACT TAATCAAGAA TCAAACGACG
    GCCTGCGATC AGAACAGCTG GAGAGGGAAC TACAGCTGTC TTAGGGTCGA TTTAATCTTT
    ACCAGAGATC GTGCATTCTA TTTCACCACC GTTTTTATAC CCGGCATTAT ATTGGTGACG
    TCGTCCTTTA TAACTTTCTG GCTAGAATGG AATGCCGTGC CAGCTAGATC GATGATAGGC
    GTGACAACAA TGCTGAATTT CTTCACTACC TCGAACGGTT TCCGGAGCAC TCTGCCGGTT
    GTTTCTAATC TGACGGCGAT GAACGTGTGG GACGGCGTGT GTATGTGCTT CATCTACGCC
    TCCTTGCTGG AATTCGTCTG CGTCAATTAT GTGGGGCGAA AGCGACCCCT GCACAACGTC
    GTCTATCGGC CCGGCGAGAA TCCCGTAACA CAGCGTCTTC CAGCAGTACT AAGCAGGATC
    GGAGTAATTC TTGCAAGTCC TTTGCCCGGC GAAAAGCGTC AAAACGCCGG GGCACCGAAC
    GAAATTGTGG CATGCACCAC CTGCGGCGGC AGCAACAGTC CGTGCACCCA TTCGGCAAAT
    AATGGATGTG CCACGGAGAC CTGCTTCGTT CAAGTGCGGA AAAAGGAGCC ACCTCATCCG
    ATTCGAGTGG CCAAGACGAT CGATGTCATC GCTAGAATTA CATTTCCAAC TGCTTATGCC
    ATTTTTCTGA TATTCTTCTT TGTACACTAT AAAGGATTTT CGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18607
    Clone ID Related Accession (Same CDS sequence) NM_001259852.1
    Accession Version NM_001259852.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GTAATTACAG TTGCCTACAG
    GTCGAGTTGA CATTTACCCG TGATCGTGCG TATTATTTTA CAACCGTTTT CATACCTGGC
    ATTATATTGG TCACCTCGTC GTTTATCACA TTTTGGCTGG AATGGAATGC AGTCCCAGCC
    CGGGTTATGA TCGGCGTGAC AACAATGCTG AATTTCTTCA CTACCTCGAA CGGTTTCCGG
    AGCACTCTGC CGGTTGTTTC TAATCTGACG GCGATGAACG TGTGGGACGG CGTGTGTATG
    TGCTTCATCT ACGCCTCCTT GCTGGAATTC GTCTGCGTCA ATTATGTGGG GCGAAAGCGA
    CCCCTGCACA ACGTCGTCTA TCGGCCCGGC GAGAATCCCG TAACACAGCC CGGCGAAAAG
    CGTCAAAACG CCGGGGCACC GAACGAAATT GTGGCATGCA CCACCTGCGG CGGCAGCAAC
    AGTCCGTGCA CCCATTCGGC AAATAATGGA TGTGCCACGG AGACCTGCTT CGTTCAAGTG
    CGGAAAAAGG AGCCACCTCA TCCGATTCGA GTGGCCAAGA CGATCGATGT CATCGCTAGA
    ATTACATTTC CAACTGCTTA TGCCATTTTT CTGATATTCT TCTTTGTACA CTATAAAGGA
    TTTTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246781.1
    CDS524..2812
    Translation

    Target ORF information:

    RefSeq Version NM_001259852.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GTAATTACAG TTGCCTACAG
    GTCGAGTTGA CATTTACCCG TGATCGTGCG TATTATTTTA CAACCGTTTT CATACCTGGC
    ATTATATTGG TCACCTCGTC GTTTATCACA TTTTGGCTGG AATGGAATGC AGTCCCAGCC
    CGGGTTATGA TCGGCGTGAC AACAATGCTG AATTTCTTCA CTACCTCGAA CGGTTTCCGG
    AGCACTCTGC CGGTTGTTTC TAATCTGACG GCGATGAACG TGTGGGACGG CGTGTGTATG
    TGCTTCATCT ACGCCTCCTT GCTGGAATTC GTCTGCGTCA ATTATGTGGG GCGAAAGCGA
    CCCCTGCACA ACGTCGTCTA TCGGCCCGGC GAGAATCCCG TAACACAGCC CGGCGAAAAG
    CGTCAAAACG CCGGGGCACC GAACGAAATT GTGGCATGCA CCACCTGCGG CGGCAGCAAC
    AGTCCGTGCA CCCATTCGGC AAATAATGGA TGTGCCACGG AGACCTGCTT CGTTCAAGTG
    CGGAAAAAGG AGCCACCTCA TCCGATTCGA GTGGCCAAGA CGATCGATGT CATCGCTAGA
    ATTACATTTC CAACTGCTTA TGCCATTTTT CTGATATTCT TCTTTGTACA CTATAAAGGA
    TTTTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18608
    Clone ID Related Accession (Same CDS sequence) NM_001274970.1
    Accession Version NM_001274970.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2790bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform O
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGGCA CTCTGACGGT GAACGTGAAC
    GTGCTGCTCC TGAGCTTAGC ATCGCCCGAC GAAAGCAGTT TGAAATACGA GGTTGAATTT
    CTGCTGAACC AGCAATGGAA CGATCCGCGA CTGCAATATG GCAATAAGTC TCATTATGAC
    TTCTTAAATG CTCTGCATCA TCATGATAGC ATCTGGACGC CGGATACGTA TTTCATTATG
    CATGGCGATT TCAAGGATCC CATCATACCA ATGCATTTCG CTTTAAGAAT ATTTCGGAAC
    GGGACCATTA CGTACGCAAT GAGACGTCAC CTGATCTTAT CCTGCCAGGG AAGTCTTCAT
    ATATTTCCAT TCGATGACCC CAAGTGTTCC TTCTCCATGG AGAGCATTTC CTACGAGGAG
    GCCCAAATCA AGTACGTCTG GAAAAACGAT GAGGACACTC TGCGGAAGAG TCCTTCGCTG
    ACCACATTGA ACGCGTACTT AATCAAGAAT CAAACGACGG CCTGCGATCA GAACAGCTGG
    AGAGGGAACT ACAGCTGTCT TAGGGTCGAT TTAATCTTTA CCAGAGATCG TGCATTCTAT
    TTCACCACCG TTTTTATACC CGGCATTATA TTGGTGACGT CGTCCTTTAT AACTTTCTGG
    CTAGAATGGA ATGCCGTGCC AGCTAGATCG ATGATAGGCG TGACAACAAT GCTGAATTTC
    TTCACTACCT CGAACGGTTT CCGGAGCACT CTGCCGGTTG TTTCTAATCT GACGGCGATG
    AACGTGTGGG ACGGCGTGTG TATGTGCTTC ATCTACGCCT CCTTGCTGGA ATTCGTCTGC
    GTCAATTATG TGGGGCGAAA GCGACCCCTG CACAACGTCG TCTATCGGCC CGGCGAGAAT
    CCCGTAACAC AGGCAAGCGC CGCTGCCACG CCGGCCATGT CGATGATGGG CGGGGCCACG
    CCCATGTCCA GTGGTACCCC GGCCGCCTCA TCCTCGGTGG CCACGCCCGG TACCCCAAGG
    ATCACCGATC CGGAGGAGTT CTTCGGCGGC GGCATGCGTT GGCAGGAGGC CCATGGCGGA
    ATCATTGGAG CTGCTGTCCA GAATTTACGG GCACGTTCGA TAAAACGTAA GGCGGCCAGA
    AGTCCATCCA CTTCCATATC GCCCCATCCC AGTGGGCGAC CCACTGAGCA TATCTACGAA
    GAGCCCGGTG GATCCACATC CGGTGTCACA AGTACGAAAG TAAAGTTCAA GGACGAGCTG
    AAGGAGGAGC AACCGGAGGA GGCGGAGTCC AGCACCAAAC TACGACGATC GGTGGCCACC
    AGTACACCGG CTTTGGTTGT AAGGATCGAG GCTGAGATCG ATCCGGAGGC GGAAGACAAG
    CCCATCCAGG ATATGGAAAT GACAGAACGT CAGTTGTCCT TGCCGTTGAA ACCGCCGCGT
    CGCAAGGCAT CGCGCTCCAA TTCACCAGCC TCGGTTTACA GCGATGCATT CGAGAATGTG
    GAAGCTACAC CCGGCGAAAA GCGTCAAAAC GCCGGGGCAC CGAACGAAAT TGTGGCATGC
    ACCACCTGCG GCGGCAGCAA CAGTCCGTGC ACCCATTCGG CAAATAATGG ATGTGCCACG
    GAGACCTGCT TCGTTCAAGT GCGGAAAAAG GAGCCACCTC ATCCGATTCG AGTGGCCAAG
    ACGATCGATG TCATCGCTAG AATTACATTT CCAACTGCTT ATGCCATTTT TCTGATATTC
    TTCTTTGTAC ACTATAAAGG ATTTTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261899.1
    CDS519..3308
    Translation

    Target ORF information:

    RefSeq Version NM_001274970.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant O, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274970.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGGCA CTCTGACGGT GAACGTGAAC
    GTGCTGCTCC TGAGCTTAGC ATCGCCCGAC GAAAGCAGTT TGAAATACGA GGTTGAATTT
    CTGCTGAACC AGCAATGGAA CGATCCGCGA CTGCAATATG GCAATAAGTC TCATTATGAC
    TTCTTAAATG CTCTGCATCA TCATGATAGC ATCTGGACGC CGGATACGTA TTTCATTATG
    CATGGCGATT TCAAGGATCC CATCATACCA ATGCATTTCG CTTTAAGAAT ATTTCGGAAC
    GGGACCATTA CGTACGCAAT GAGACGTCAC CTGATCTTAT CCTGCCAGGG AAGTCTTCAT
    ATATTTCCAT TCGATGACCC CAAGTGTTCC TTCTCCATGG AGAGCATTTC CTACGAGGAG
    GCCCAAATCA AGTACGTCTG GAAAAACGAT GAGGACACTC TGCGGAAGAG TCCTTCGCTG
    ACCACATTGA ACGCGTACTT AATCAAGAAT CAAACGACGG CCTGCGATCA GAACAGCTGG
    AGAGGGAACT ACAGCTGTCT TAGGGTCGAT TTAATCTTTA CCAGAGATCG TGCATTCTAT
    TTCACCACCG TTTTTATACC CGGCATTATA TTGGTGACGT CGTCCTTTAT AACTTTCTGG
    CTAGAATGGA ATGCCGTGCC AGCTAGATCG ATGATAGGCG TGACAACAAT GCTGAATTTC
    TTCACTACCT CGAACGGTTT CCGGAGCACT CTGCCGGTTG TTTCTAATCT GACGGCGATG
    AACGTGTGGG ACGGCGTGTG TATGTGCTTC ATCTACGCCT CCTTGCTGGA ATTCGTCTGC
    GTCAATTATG TGGGGCGAAA GCGACCCCTG CACAACGTCG TCTATCGGCC CGGCGAGAAT
    CCCGTAACAC AGGCAAGCGC CGCTGCCACG CCGGCCATGT CGATGATGGG CGGGGCCACG
    CCCATGTCCA GTGGTACCCC GGCCGCCTCA TCCTCGGTGG CCACGCCCGG TACCCCAAGG
    ATCACCGATC CGGAGGAGTT CTTCGGCGGC GGCATGCGTT GGCAGGAGGC CCATGGCGGA
    ATCATTGGAG CTGCTGTCCA GAATTTACGG GCACGTTCGA TAAAACGTAA GGCGGCCAGA
    AGTCCATCCA CTTCCATATC GCCCCATCCC AGTGGGCGAC CCACTGAGCA TATCTACGAA
    GAGCCCGGTG GATCCACATC CGGTGTCACA AGTACGAAAG TAAAGTTCAA GGACGAGCTG
    AAGGAGGAGC AACCGGAGGA GGCGGAGTCC AGCACCAAAC TACGACGATC GGTGGCCACC
    AGTACACCGG CTTTGGTTGT AAGGATCGAG GCTGAGATCG ATCCGGAGGC GGAAGACAAG
    CCCATCCAGG ATATGGAAAT GACAGAACGT CAGTTGTCCT TGCCGTTGAA ACCGCCGCGT
    CGCAAGGCAT CGCGCTCCAA TTCACCAGCC TCGGTTTACA GCGATGCATT CGAGAATGTG
    GAAGCTACAC CCGGCGAAAA GCGTCAAAAC GCCGGGGCAC CGAACGAAAT TGTGGCATGC
    ACCACCTGCG GCGGCAGCAA CAGTCCGTGC ACCCATTCGG CAAATAATGG ATGTGCCACG
    GAGACCTGCT TCGTTCAAGT GCGGAAAAAG GAGCCACCTC ATCCGATTCG AGTGGCCAAG
    ACGATCGATG TCATCGCTAG AATTACATTT CCAACTGCTT ATGCCATTTT TCTGATATTC
    TTCTTTGTAC ACTATAAAGG ATTTTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18609
    Clone ID Related Accession (Same CDS sequence) NM_001274971.1
    Accession Version NM_001274971.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform P
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGATG AAGACTTTTG CTGTGGCTTG
    CAATCGCCCG ATATGGCAAC TAATCAAAAT GCACGGAGAC CACACAATCG CGGCACTCTG
    ACGGTGAACG TGAACGTGCT GCTCCTGAGC TTAGCATCGC CCGACGAAAG CAGTTTGAAA
    TACGAGGTTG AATTTCTGCT GAACCAGCAA TGGAACGATC CGCGACTGCA ATATGGCAAT
    AAGTCTCATT ATGACTTCTT AAATGCTCTG CATCATCATG ATAGCATCTG GACGCCGGAT
    ACGTATTTCA TTATGCATGG CGATTTCAAG GATCCCATCA TACCAATGCA TTTCGCTTTA
    AGAATATTTC GGAACGGGAC CATTACGTAC GCAATGAGAC GTCACCTGAT CTTATCCTGC
    CAGGGAAGTC TTCATATATT TCCATTCGAT GACCCCAAGT GTTCCTTCTC CATGGAGAGC
    ATTTCCTACG AGGAGGCCCA AATCAAGTAC GTCTGGAAAA ACGATGAGGA CACTCTGCGG
    AAGAGTCCTT CGCTGACCAC ATTGAACGCG TACTTAATCA AGAATCAAAC GACGGCCTGC
    GATCAGAACA GCTGGAGAGG TAATTACAGT TGCCTACAGG TCGAGTTGAC ATTTACCCGT
    GATCGTGCGT ATTATTTTAC AACCGTTTTC ATACCTGGCA TTATATTGGT CACCTCGTCG
    TTTATCACAT TTTGGCTGGA ATGGAATGCA GTCCCAGCCC GGGTTATGAT CGGCGTGACA
    ACAATGCTGA ATTTCTTCAC TACCTCGAAC GGTTTCCGGA GCACTCTGCC GGTTGTTTCT
    AATCTGACGG CGATGAACGT GTGGGACGGC GTGTGTATGT GCTTCATCTA CGCCTCCTTG
    CTGGAATTCG TCTGCGTCAA TTATGTGGGG CGAAAGCGAC CCCTGCACAA CGTCGTCTAT
    CGGCCCGGCG AGAATCCCGT AACACAGGGC GAAAAGCGTC AAAACGCCGG GGCACCGAAC
    GAAATTGTGG CATGCACCAC CTGCGGCGGC AGCAACAGTC CGTGCACCCA TTCGGCAAAT
    AATGGATGTG CCACGGAGAC CTGCTTCGTT CAAGTGCGGA AAAAGGAGCC ACCTCATCCG
    ATTCGAGTGG CCAAGACGAT CGATGTCATC GCTAGAATTA CATTTCCAAC TGCTTATGCC
    ATTTTTCTGA TATTCTTCTT TGTACACTAT AAAGGATTTT CGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261900.1
    CDS519..2783
    Translation

    Target ORF information:

    RefSeq Version NM_001274971.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant P, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274971.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGATG AAGACTTTTG CTGTGGCTTG
    CAATCGCCCG ATATGGCAAC TAATCAAAAT GCACGGAGAC CACACAATCG CGGCACTCTG
    ACGGTGAACG TGAACGTGCT GCTCCTGAGC TTAGCATCGC CCGACGAAAG CAGTTTGAAA
    TACGAGGTTG AATTTCTGCT GAACCAGCAA TGGAACGATC CGCGACTGCA ATATGGCAAT
    AAGTCTCATT ATGACTTCTT AAATGCTCTG CATCATCATG ATAGCATCTG GACGCCGGAT
    ACGTATTTCA TTATGCATGG CGATTTCAAG GATCCCATCA TACCAATGCA TTTCGCTTTA
    AGAATATTTC GGAACGGGAC CATTACGTAC GCAATGAGAC GTCACCTGAT CTTATCCTGC
    CAGGGAAGTC TTCATATATT TCCATTCGAT GACCCCAAGT GTTCCTTCTC CATGGAGAGC
    ATTTCCTACG AGGAGGCCCA AATCAAGTAC GTCTGGAAAA ACGATGAGGA CACTCTGCGG
    AAGAGTCCTT CGCTGACCAC ATTGAACGCG TACTTAATCA AGAATCAAAC GACGGCCTGC
    GATCAGAACA GCTGGAGAGG TAATTACAGT TGCCTACAGG TCGAGTTGAC ATTTACCCGT
    GATCGTGCGT ATTATTTTAC AACCGTTTTC ATACCTGGCA TTATATTGGT CACCTCGTCG
    TTTATCACAT TTTGGCTGGA ATGGAATGCA GTCCCAGCCC GGGTTATGAT CGGCGTGACA
    ACAATGCTGA ATTTCTTCAC TACCTCGAAC GGTTTCCGGA GCACTCTGCC GGTTGTTTCT
    AATCTGACGG CGATGAACGT GTGGGACGGC GTGTGTATGT GCTTCATCTA CGCCTCCTTG
    CTGGAATTCG TCTGCGTCAA TTATGTGGGG CGAAAGCGAC CCCTGCACAA CGTCGTCTAT
    CGGCCCGGCG AGAATCCCGT AACACAGGGC GAAAAGCGTC AAAACGCCGG GGCACCGAAC
    GAAATTGTGG CATGCACCAC CTGCGGCGGC AGCAACAGTC CGTGCACCCA TTCGGCAAAT
    AATGGATGTG CCACGGAGAC CTGCTTCGTT CAAGTGCGGA AAAAGGAGCC ACCTCATCCG
    ATTCGAGTGG CCAAGACGAT CGATGTCATC GCTAGAATTA CATTTCCAAC TGCTTATGCC
    ATTTTTCTGA TATTCTTCTT TGTACACTAT AAAGGATTTT CGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18610
    Clone ID Related Accession (Same CDS sequence) NM_001274968.1
    Accession Version NM_001274968.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2865bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGATG AAGACTTTTG CTGTGGCTTG
    CAATCGCCCG ATATGGCAAC TAATCAAAAT GCACGGAGAC CACACAATCG CGGCACTCTG
    ACGGTGAACG TGAACGTGCT GCTCCTGAGC TTAGCATCGC CCGACGAAAG CAGTTTGAAA
    TACGAGGTTG AATTTCTGCT GAACCAGCAA TGGAACGATC CGCGACTGCA ATATGGCAAT
    AAGTCTCATT ATGACTTCTT AAATGCTCTG CATCATCATG ATAGCATCTG GACGCCGGAT
    ACGTATTTCA TTATGCATGG CGATTTCAAG GATCCCATCA TACCAATGCA TTTCGCTTTA
    AGAATATTTC GGAACGGGAC CATTACGTAC GCAATGAGAC GTCACCTGAT CTTATCCTGC
    CAGGGAAGTC TTCATATATT TCCATTCGAT GACCCCAAGT GTTCCTTCTC CATGGAGAGC
    ATTTCCTACG AGGAGGCCCA AATCAAGTAC GTCTGGAAAA ACGATGAGGA CACTCTGCGG
    AAGAGTCCTT CGCTGACCAC ATTGAACGCG TACTTAATCA AGAATCAAAC GACGGCCTGC
    GATCAGAACA GCTGGAGAGG TAATTACAGT TGCCTACAGG TCGAGTTGAC ATTTACCCGT
    GATCGTGCGT ATTATTTTAC AACCGTTTTC ATACCTGGCA TTATATTGGT CACCTCGTCG
    TTTATCACAT TTTGGCTGGA ATGGAATGCA GTCCCAGCCC GGGTTATGAT CGGCGTGACA
    ACAATGCTGA ATTTCTTCAC TACCTCGAAC GGTTTCCGGA GCACTCTGCC GGTTGTTTCT
    AATCTGACGG CGATGAACGT GTGGGACGGC GTGTGTATGT GCTTCATCTA CGCCTCCTTG
    CTGGAATTCG TCTGCGTCAA TTATGTGGGG CGAAAGCGAC CCCTGCACAA CGTCGTCTAT
    CGGCCCGGCG AGAATCCCGT AACACAGGCA AGCGCCGCTG CCACGCCGGC CATGTCGATG
    ATGGGCGGGG CCACGCCCAT GTCCAGTGGT ACCCCGGCCG CCTCATCCTC GGTGGCCACG
    CCCGGTACCC CAAGGATCAC CGATCCGGAG GAGTTCTTCG GCGGCGGCAT GCGTTGGCAG
    GAGGCCCATG GCGGAATCAT TGGAGCTGCT GTCCAGAATT TACGGGCACG TTCGATAAAA
    CGTAAGGCGG CCAGAAGTCC ATCCACTTCC ATATCGCCCC ATCCCAGTGG GCGACCCACT
    GAGCATATCT ACGAAGAGCC CGGTGGATCC ACATCCGGTG TCACAAGTAC GAAAGTAAAG
    TTCAAGGACG AGCTGAAGGA GGAGCAACCG GAGGAGGCGG AGTCCAGCAC CAAACTACGA
    CGATCGGTGG CCACCAGTAC ACCGGCTTTG GTTGTAAGGA TCGAGGCTGA GATCGATCCG
    GAGGCGGAAG ACAAGCCCAT CCAGGATATG GAAATGACAG AACGTCAGTT GTCCTTGCCG
    TTGAAACCGC CGCGTCGCAA GGCATCGCGC TCCAATTCAC CAGCCTCGGT TTACAGCGAT
    GCATTCGAGA ATGTGGAAGC TACACCCGGC GAAAAGCGTC AAAACGCCGG GGCACCGAAC
    GAAATTGTGG CATGCACCAC CTGCGGCGGC AGCAACAGTC CGTGCACCCA TTCGGCAAAT
    AATGGATGTG CCACGGAGAC CTGCTTCGTT CAAGTGCGGA AAAAGGAGCC ACCTCATCCG
    ATTCGAGTGG CCAAGACGAT CGATGTCATC GCTAGAATTA CATTTCCAAC TGCTTATGCC
    ATTTTTCTGA TATTCTTCTT TGTACACTAT AAAGGATTTT CGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261897.1
    CDS519..3383
    Translation

    Target ORF information:

    RefSeq Version NM_001274968.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant M, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274968.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGATG AAGACTTTTG CTGTGGCTTG
    CAATCGCCCG ATATGGCAAC TAATCAAAAT GCACGGAGAC CACACAATCG CGGCACTCTG
    ACGGTGAACG TGAACGTGCT GCTCCTGAGC TTAGCATCGC CCGACGAAAG CAGTTTGAAA
    TACGAGGTTG AATTTCTGCT GAACCAGCAA TGGAACGATC CGCGACTGCA ATATGGCAAT
    AAGTCTCATT ATGACTTCTT AAATGCTCTG CATCATCATG ATAGCATCTG GACGCCGGAT
    ACGTATTTCA TTATGCATGG CGATTTCAAG GATCCCATCA TACCAATGCA TTTCGCTTTA
    AGAATATTTC GGAACGGGAC CATTACGTAC GCAATGAGAC GTCACCTGAT CTTATCCTGC
    CAGGGAAGTC TTCATATATT TCCATTCGAT GACCCCAAGT GTTCCTTCTC CATGGAGAGC
    ATTTCCTACG AGGAGGCCCA AATCAAGTAC GTCTGGAAAA ACGATGAGGA CACTCTGCGG
    AAGAGTCCTT CGCTGACCAC ATTGAACGCG TACTTAATCA AGAATCAAAC GACGGCCTGC
    GATCAGAACA GCTGGAGAGG TAATTACAGT TGCCTACAGG TCGAGTTGAC ATTTACCCGT
    GATCGTGCGT ATTATTTTAC AACCGTTTTC ATACCTGGCA TTATATTGGT CACCTCGTCG
    TTTATCACAT TTTGGCTGGA ATGGAATGCA GTCCCAGCCC GGGTTATGAT CGGCGTGACA
    ACAATGCTGA ATTTCTTCAC TACCTCGAAC GGTTTCCGGA GCACTCTGCC GGTTGTTTCT
    AATCTGACGG CGATGAACGT GTGGGACGGC GTGTGTATGT GCTTCATCTA CGCCTCCTTG
    CTGGAATTCG TCTGCGTCAA TTATGTGGGG CGAAAGCGAC CCCTGCACAA CGTCGTCTAT
    CGGCCCGGCG AGAATCCCGT AACACAGGCA AGCGCCGCTG CCACGCCGGC CATGTCGATG
    ATGGGCGGGG CCACGCCCAT GTCCAGTGGT ACCCCGGCCG CCTCATCCTC GGTGGCCACG
    CCCGGTACCC CAAGGATCAC CGATCCGGAG GAGTTCTTCG GCGGCGGCAT GCGTTGGCAG
    GAGGCCCATG GCGGAATCAT TGGAGCTGCT GTCCAGAATT TACGGGCACG TTCGATAAAA
    CGTAAGGCGG CCAGAAGTCC ATCCACTTCC ATATCGCCCC ATCCCAGTGG GCGACCCACT
    GAGCATATCT ACGAAGAGCC CGGTGGATCC ACATCCGGTG TCACAAGTAC GAAAGTAAAG
    TTCAAGGACG AGCTGAAGGA GGAGCAACCG GAGGAGGCGG AGTCCAGCAC CAAACTACGA
    CGATCGGTGG CCACCAGTAC ACCGGCTTTG GTTGTAAGGA TCGAGGCTGA GATCGATCCG
    GAGGCGGAAG ACAAGCCCAT CCAGGATATG GAAATGACAG AACGTCAGTT GTCCTTGCCG
    TTGAAACCGC CGCGTCGCAA GGCATCGCGC TCCAATTCAC CAGCCTCGGT TTACAGCGAT
    GCATTCGAGA ATGTGGAAGC TACACCCGGC GAAAAGCGTC AAAACGCCGG GGCACCGAAC
    GAAATTGTGG CATGCACCAC CTGCGGCGGC AGCAACAGTC CGTGCACCCA TTCGGCAAAT
    AATGGATGTG CCACGGAGAC CTGCTTCGTT CAAGTGCGGA AAAAGGAGCC ACCTCATCCG
    ATTCGAGTGG CCAAGACGAT CGATGTCATC GCTAGAATTA CATTTCCAAC TGCTTATGCC
    ATTTTTCTGA TATTCTTCTT TGTACACTAT AAAGGATTTT CGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18611
    Clone ID Related Accession (Same CDS sequence) NM_001169979.1
    Accession Version NM_001169979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GGAACTACAG CTGTCTTAGG
    GTCGATTTAA TCTTTACCAG AGATCGTGCA TTCTATTTCA CCACCGTTTT TATACCCGGC
    ATTATATTGG TGACGTCGTC CTTTATAACT TTCTGGCTAG AATGGAATGC CGTGCCAGCT
    AGATCGATGA TAGGCGTGAC AACAATGCTG AATTTCTTCA CTACCTCGAA CGGTTTCCGG
    AGCACTCTGC CGGTTGTTTC TAATCTGACG GCGATGAACG TGTGGGACGG CGTGTGTATG
    TGCTTCATCT ACGCCTCCTT GCTGGAATTC GTCTGCGTCA ATTATGTGGG GCGAAAGCGA
    CCCCTGCACA ACGTCGTCTA TCGGCCCGGC GAGAATCCCG TAACACAGCC CGTAAGTATG
    CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163450.1
    CDS519..2564
    Translation

    Target ORF information:

    RefSeq Version NM_001169979.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GGAACTACAG CTGTCTTAGG
    GTCGATTTAA TCTTTACCAG AGATCGTGCA TTCTATTTCA CCACCGTTTT TATACCCGGC
    ATTATATTGG TGACGTCGTC CTTTATAACT TTCTGGCTAG AATGGAATGC CGTGCCAGCT
    AGATCGATGA TAGGCGTGAC AACAATGCTG AATTTCTTCA CTACCTCGAA CGGTTTCCGG
    AGCACTCTGC CGGTTGTTTC TAATCTGACG GCGATGAACG TGTGGGACGG CGTGTGTATG
    TGCTTCATCT ACGCCTCCTT GCTGGAATTC GTCTGCGTCA ATTATGTGGG GCGAAAGCGA
    CCCCTGCACA ACGTCGTCTA TCGGCCCGGC GAGAATCCCG TAACACAGCC CGTAAGTATG
    CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20950
    Clone ID Related Accession (Same CDS sequence) NM_001038934.4
    Accession Version NM_001038934.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2214bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001038934.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGGC
    ACTCTGACGG TGAACGTGAA CGTGCTGCTC CTGAGCTTAG CATCGCCCGA CGAAAGCAGT
    TTGAAATACG AGGTTGAATT TCTGCTGAAC CAGCAATGGA ACGATCCGCG ACTGCAATAT
    GGCAATAAGT CTCATTATGA CTTCTTAAAT GCTCTGCATC ATCATGATAG CATCTGGACG
    CCGGATACGT ATTTCATTAT GCATGGCGAT TTCAAGGATC CCATCATACC AATGCATTTC
    GCTTTAAGAA TATTTCGGAA CGGGACCATT ACGTACGCAA TGAGACGTCA CCTGATCTTA
    TCCTGCCAGG GAAGTCTTCA TATATTTCCA TTCGATGACC CCAAGTGTTC CTTCTCCATG
    GAGAGCATTT CCTACGAGGA GGCCCAAATC AAGTACGTCT GGAAAAACGA TGAGGACACT
    CTGCGGAAGA GTCCTTCGCT GACCACATTG AACGCGTACT TAATCAAGAA TCAAACGACG
    GCCTGCGATC AGAACAGCTG GAGAGGTAAT TACAGTTGCC TACAGGTCGA GTTGACATTT
    ACCCGTGATC GTGCGTATTA TTTTACAACC GTTTTCATAC CTGGCATTAT ATTGGTCACC
    TCGTCGTTTA TCACATTTTG GCTGGAATGG AATGCAGTCC CAGCCCGGGT TATGATCGGC
    GTGACAACAA TGCTGAATTT CTTCACTACC TCGAACGGTT TCCGGAGCAC TCTGCCGGTT
    GTTTCTAATC TGACGGCGAT GAACGTGTGG GACGGCGTGT GTATGTGCTT CATCTACGCC
    TCCTTGCTGG AATTCGTCTG CGTCAATTAT GTGGGGCGAA AGCGACCCCT GCACAACGTC
    GTCTATCGGC CCGGCGAGAA TCCCGTAACA CAGCCCGGCG AAAAGCGTCA AAACGCCGGG
    GCACCGAACG AAATTGTGGC ATGCACCACC TGCGGCGGCA GCAACAGTCC GTGCACCCAT
    TCGGCAAATA ATGGATGTGC CACGGAGACC TGCTTCGTTC AAGTGCGGAA AAAGGAGCCA
    CCTCATCCGA TTCGAGTGGC CAAGACGATC GATGTCATCG CTAGAATTAC ATTTCCAACT
    GCTTATGCCA TTTTTCTGAT ATTCTTCTTT GTACACTATA AAGGATTTTC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001034023.2
    CDS516..2729
    Translation

    Target ORF information:

    RefSeq Version NM_001038934.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001038934.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGGC
    ACTCTGACGG TGAACGTGAA CGTGCTGCTC CTGAGCTTAG CATCGCCCGA CGAAAGCAGT
    TTGAAATACG AGGTTGAATT TCTGCTGAAC CAGCAATGGA ACGATCCGCG ACTGCAATAT
    GGCAATAAGT CTCATTATGA CTTCTTAAAT GCTCTGCATC ATCATGATAG CATCTGGACG
    CCGGATACGT ATTTCATTAT GCATGGCGAT TTCAAGGATC CCATCATACC AATGCATTTC
    GCTTTAAGAA TATTTCGGAA CGGGACCATT ACGTACGCAA TGAGACGTCA CCTGATCTTA
    TCCTGCCAGG GAAGTCTTCA TATATTTCCA TTCGATGACC CCAAGTGTTC CTTCTCCATG
    GAGAGCATTT CCTACGAGGA GGCCCAAATC AAGTACGTCT GGAAAAACGA TGAGGACACT
    CTGCGGAAGA GTCCTTCGCT GACCACATTG AACGCGTACT TAATCAAGAA TCAAACGACG
    GCCTGCGATC AGAACAGCTG GAGAGGTAAT TACAGTTGCC TACAGGTCGA GTTGACATTT
    ACCCGTGATC GTGCGTATTA TTTTACAACC GTTTTCATAC CTGGCATTAT ATTGGTCACC
    TCGTCGTTTA TCACATTTTG GCTGGAATGG AATGCAGTCC CAGCCCGGGT TATGATCGGC
    GTGACAACAA TGCTGAATTT CTTCACTACC TCGAACGGTT TCCGGAGCAC TCTGCCGGTT
    GTTTCTAATC TGACGGCGAT GAACGTGTGG GACGGCGTGT GTATGTGCTT CATCTACGCC
    TCCTTGCTGG AATTCGTCTG CGTCAATTAT GTGGGGCGAA AGCGACCCCT GCACAACGTC
    GTCTATCGGC CCGGCGAGAA TCCCGTAACA CAGCCCGGCG AAAAGCGTCA AAACGCCGGG
    GCACCGAACG AAATTGTGGC ATGCACCACC TGCGGCGGCA GCAACAGTCC GTGCACCCAT
    TCGGCAAATA ATGGATGTGC CACGGAGACC TGCTTCGTTC AAGTGCGGAA AAAGGAGCCA
    CCTCATCCGA TTCGAGTGGC CAAGACGATC GATGTCATCG CTAGAATTAC ATTTCCAACT
    GCTTATGCCA TTTTTCTGAT ATTCTTCTTT GTACACTATA AAGGATTTTC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20951
    Clone ID Related Accession (Same CDS sequence) NM_001038936.4
    Accession Version NM_001038936.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001038936.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GGAACTACAG CTGTCTTAGG
    GTCGATTTAA TCTTTACCAG AGATCGTGCA TTCTATTTCA CCACCGTTTT TATACCCGGC
    ATTATATTGG TGACGTCGTC CTTTATAACT TTCTGGCTAG AATGGAATGC CGTGCCAGCT
    AGATCGATGA TAGGCGTGAC AACAATGCTG AATTTCTTCA CTACCTCGAA CGGTTTCCGG
    AGCACTCTGC CGGTTGTTTC TAATCTGACG GCGATGAACG TGTGGGACGG CGTGTGTATG
    TGCTTCATCT ACGCCTCCTT GCTGGAATTC GTCTGCGTCA ATTATGTGGG GCGAAAGCGA
    CCCCTGCACA ACGTCGTCTA TCGGCCCGGC GAGAATCCCG TAACACAGCC CGGCGAAAAG
    CGTCAAAACG CCGGGGCACC GAACGAAATT GTGGCATGCA CCACCTGCGG CGGCAGCAAC
    AGTCCGTGCA CCCATTCGGC AAATAATGGA TGTGCCACGG AGACCTGCTT CGTTCAAGTG
    CGGAAAAAGG AGCCACCTCA TCCGATTCGA GTGGCCAAGA CGATCGATGT CATCGCTAGA
    ATTACATTTC CAACTGCTTA TGCCATTTTT CTGATATTCT TCTTTGTACA CTATAAAGGA
    TTTTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001034025.2
    CDS516..2804
    Translation

    Target ORF information:

    RefSeq Version NM_001038936.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001038936.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GGAACTACAG CTGTCTTAGG
    GTCGATTTAA TCTTTACCAG AGATCGTGCA TTCTATTTCA CCACCGTTTT TATACCCGGC
    ATTATATTGG TGACGTCGTC CTTTATAACT TTCTGGCTAG AATGGAATGC CGTGCCAGCT
    AGATCGATGA TAGGCGTGAC AACAATGCTG AATTTCTTCA CTACCTCGAA CGGTTTCCGG
    AGCACTCTGC CGGTTGTTTC TAATCTGACG GCGATGAACG TGTGGGACGG CGTGTGTATG
    TGCTTCATCT ACGCCTCCTT GCTGGAATTC GTCTGCGTCA ATTATGTGGG GCGAAAGCGA
    CCCCTGCACA ACGTCGTCTA TCGGCCCGGC GAGAATCCCG TAACACAGCC CGGCGAAAAG
    CGTCAAAACG CCGGGGCACC GAACGAAATT GTGGCATGCA CCACCTGCGG CGGCAGCAAC
    AGTCCGTGCA CCCATTCGGC AAATAATGGA TGTGCCACGG AGACCTGCTT CGTTCAAGTG
    CGGAAAAAGG AGCCACCTCA TCCGATTCGA GTGGCCAAGA CGATCGATGT CATCGCTAGA
    ATTACATTTC CAACTGCTTA TGCCATTTTT CTGATATTCT TCTTTGTACA CTATAAAGGA
    TTTTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20952
    Clone ID Related Accession (Same CDS sequence) NM_001038935.3
    Accession Version NM_001038935.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_001038935.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GGAACTACAG CTGTCTTAGG
    GTCGATTTAA TCTTTACCAG AGATCGTGCA TTCTATTTCA CCACCGTTTT TATACCCGGC
    ATTATATTGG TGACGTCGTC CTTTATAACT TTCTGGCTAG AATGGAATGC CGTGCCAGCT
    AGATCGATGA TAGGCGTGAC AACAATGCTG AATTTCTTCA CTACCTCGAA CGGTTTCCGG
    AGCACTCTGC CGGTTGTTTC TAATCTGACG GCGATGAACG TGTGGGACGG CGTGTGTATG
    TGCTTCATCT ACGCCTCCTT GCTGGAATTC GTCTGCGTCA ATTATGTGGG GCGAAAGCGA
    CCCCTGCACA ACGTCGTCTA TCGGCCCGGC GAGAATCCCG TAACACAGCG TCTTCCAGCA
    GTACTAAGCA GGATCGGAGT AATTCTTGCA AGTCCTTTGC CCGGCGAAAA GCGTCAAAAC
    GCCGGGGCAC CGAACGAAAT TGTGGCATGC ACCACCTGCG GCGGCAGCAA CAGTCCGTGC
    ACCCATTCGG CAAATAATGG ATGTGCCACG GAGACCTGCT TCGTTCAAGT GCGGAAAAAG
    GAGCCACCTC ATCCGATTCG AGTGGCCAAG ACGATCGATG TCATCGCTAG AATTACATTT
    CCAACTGCTT ATGCCATTTT TCTGATATTC TTCTTTGTAC ACTATAAAGG ATTTTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001034024.2
    CDS519..2858
    Translation

    Target ORF information:

    RefSeq Version NM_001038935.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001038935.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GGAACTACAG CTGTCTTAGG
    GTCGATTTAA TCTTTACCAG AGATCGTGCA TTCTATTTCA CCACCGTTTT TATACCCGGC
    ATTATATTGG TGACGTCGTC CTTTATAACT TTCTGGCTAG AATGGAATGC CGTGCCAGCT
    AGATCGATGA TAGGCGTGAC AACAATGCTG AATTTCTTCA CTACCTCGAA CGGTTTCCGG
    AGCACTCTGC CGGTTGTTTC TAATCTGACG GCGATGAACG TGTGGGACGG CGTGTGTATG
    TGCTTCATCT ACGCCTCCTT GCTGGAATTC GTCTGCGTCA ATTATGTGGG GCGAAAGCGA
    CCCCTGCACA ACGTCGTCTA TCGGCCCGGC GAGAATCCCG TAACACAGCG TCTTCCAGCA
    GTACTAAGCA GGATCGGAGT AATTCTTGCA AGTCCTTTGC CCGGCGAAAA GCGTCAAAAC
    GCCGGGGCAC CGAACGAAAT TGTGGCATGC ACCACCTGCG GCGGCAGCAA CAGTCCGTGC
    ACCCATTCGG CAAATAATGG ATGTGCCACG GAGACCTGCT TCGTTCAAGT GCGGAAAAAG
    GAGCCACCTC ATCCGATTCG AGTGGCCAAG ACGATCGATG TCATCGCTAG AATTACATTT
    CCAACTGCTT ATGCCATTTT TCTGATATTC TTCTTTGTAC ACTATAAAGG ATTTTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa22021
    Clone ID Related Accession (Same CDS sequence) NM_001300146.1
    Accession Version NM_001300146.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-08-15
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform Q
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.18 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGGCA CTCTGACGGT GAACGTGAAC
    GTGCTGCTCC TGAGCTTAGC ATCGCCCGAC GAAAGCAGTT TGAAATACGA GGTTGAATTT
    CTGCTGAACC AGCAATGGAA CGATCCGCGA CTGCAATATG GCAATAAGTC TCATTATGAC
    TTCTTAAATG CTCTGCATCA TCATGATAGC ATCTGGACGC CGGATACGTA TTTCATTATG
    CATGGCGATT TCAAGGATCC CATCATACCA ATGCATTTCG CTTTAAGAAT ATTTCGGAAC
    GGGACCATTA CGTACGCAAT GAGACGTCAC CTGATCTTAT CCTGCCAGGG AAGTCTTCAT
    ATATTTCCAT TCGATGACCC CAAGTGTTCC TTCTCCATGG AGAGCATTTC CTACGAGGAG
    GCCCAAATCA AGTACGTCTG GAAAAACGAT GAGGACACTC TGCGGAAGAG TCCTTCGCTG
    ACCACATTGA ACGCGTACTT AATCAAGAAT CAAACGACGG CCTGCGATCA GAACAGCTGG
    AGAGGTAATT ACAGTTGCCT ACAGGTCGAG TTGACATTTA CCCGTGATCG TGCGTATTAT
    TTTACAACCG TTTTCATACC TGGCATTATA TTGGTCACCT CGTCGTTTAT CACATTTTGG
    CTGGAATGGA ATGCAGTCCC AGCCCGGGTT ATGATCGGCG TGACAACAAT GCTGAATTTC
    TTCACTACCT CGAACGGTTT CCGGAGCACT CTGCCGGTTG TTTCTAATCT GACGGCGATG
    AACGTGTGGG ACGGCGTGTG TATGTGCTTC ATCTACGCCT CCTTGCTGGA ATTCGTCTGC
    GTCAATTATG TGGGGCGAAA GCGACCCCTG CACAACGTCG TCTATCGGCC CGGCGAGAAT
    CCCGTAACAC AGCGTCTTCC AGCAGTACTA AGCAGGATCG GAGTAATTCT TGCAAGTCCT
    TTGCAGGGCG AAAAGCGTCA AAACGCCGGG GCACCGAACG AAATTGTGGC ATGCACCACC
    TGCGGCGGCA GCAACAGTCC GTGCACCCAT TCGGCAAATA ATGGATGTGC CACGGAGACC
    TGCTTCGTTC AAGTGCGGAA AAAGGAGCCA CCTCATCCGA TTCGAGTGGC CAAGACGATC
    GATGTCATCG CTAGAATTAC ATTTCCAACT GCTTATGCCA TTTTTCTGAT ATTCTTCTTT
    GTACACTATA AAGGATTTTC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287075.1
    CDS524..2767
    Translation

    Target ORF information:

    RefSeq Version NM_001300146.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant Q, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300146.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGGCA CTCTGACGGT GAACGTGAAC
    GTGCTGCTCC TGAGCTTAGC ATCGCCCGAC GAAAGCAGTT TGAAATACGA GGTTGAATTT
    CTGCTGAACC AGCAATGGAA CGATCCGCGA CTGCAATATG GCAATAAGTC TCATTATGAC
    TTCTTAAATG CTCTGCATCA TCATGATAGC ATCTGGACGC CGGATACGTA TTTCATTATG
    CATGGCGATT TCAAGGATCC CATCATACCA ATGCATTTCG CTTTAAGAAT ATTTCGGAAC
    GGGACCATTA CGTACGCAAT GAGACGTCAC CTGATCTTAT CCTGCCAGGG AAGTCTTCAT
    ATATTTCCAT TCGATGACCC CAAGTGTTCC TTCTCCATGG AGAGCATTTC CTACGAGGAG
    GCCCAAATCA AGTACGTCTG GAAAAACGAT GAGGACACTC TGCGGAAGAG TCCTTCGCTG
    ACCACATTGA ACGCGTACTT AATCAAGAAT CAAACGACGG CCTGCGATCA GAACAGCTGG
    AGAGGTAATT ACAGTTGCCT ACAGGTCGAG TTGACATTTA CCCGTGATCG TGCGTATTAT
    TTTACAACCG TTTTCATACC TGGCATTATA TTGGTCACCT CGTCGTTTAT CACATTTTGG
    CTGGAATGGA ATGCAGTCCC AGCCCGGGTT ATGATCGGCG TGACAACAAT GCTGAATTTC
    TTCACTACCT CGAACGGTTT CCGGAGCACT CTGCCGGTTG TTTCTAATCT GACGGCGATG
    AACGTGTGGG ACGGCGTGTG TATGTGCTTC ATCTACGCCT CCTTGCTGGA ATTCGTCTGC
    GTCAATTATG TGGGGCGAAA GCGACCCCTG CACAACGTCG TCTATCGGCC CGGCGAGAAT
    CCCGTAACAC AGCGTCTTCC AGCAGTACTA AGCAGGATCG GAGTAATTCT TGCAAGTCCT
    TTGCAGGGCG AAAAGCGTCA AAACGCCGGG GCACCGAACG AAATTGTGGC ATGCACCACC
    TGCGGCGGCA GCAACAGTCC GTGCACCCAT TCGGCAAATA ATGGATGTGC CACGGAGACC
    TGCTTCGTTC AAGTGCGGAA AAAGGAGCCA CCTCATCCGA TTCGAGTGGC CAAGACGATC
    GATGTCATCG CTAGAATTAC ATTTCCAACT GCTTATGCCA TTTTTCTGAT ATTCTTCTTT
    GTACACTATA AAGGATTTTC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa22022
    Clone ID Related Accession (Same CDS sequence) NM_001300147.1
    Accession Version NM_001300147.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-08-15
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform R
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.18 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GGAACTACAG CTGTCTTAGG
    GTCGATTTAA TCTTTACCAG AGATCGTGCA TTCTATTTCA CCACCGTTTT TATACCCGGC
    ATTATATTGG TGACGTCGTC CTTTATAACT TTCTGGCTAG AATGGAATGC CGTGCCAGCT
    AGATCGATGA TAGGCGTGAC AACAATGCTG AATTTCTTCA CTACCTCGAA CGGTTTCCGG
    AGCACTCTGC CGGTTGTTTC TAATCTGACG GCGATGAACG TGTGGGACGG CGTGTGTATG
    TGCTTCATCT ACGCCTCCTT GCTGGAATTC GTCTGCGTCA ATTATGTGGG GCGAAAGCGA
    CCCCTGCACA ACGTCGTCTA TCGGCCCGGC GAGAATCCCG TAACACAGCA GGGCGAAAAG
    CGTCAAAACG CCGGGGCACC GAACGAAATT GTGGCATGCA CCACCTGCGG CGGCAGCAAC
    AGTCCGTGCA CCCATTCGGC AAATAATGGA TGTGCCACGG AGACCTGCTT CGTTCAAGTG
    CGGAAAAAGG AGCCACCTCA TCCGATTCGA GTGGCCAAGA CGATCGATGT CATCGCTAGA
    ATTACATTTC CAACTGCTTA TGCCATTTTT CTGATATTCT TCTTTGTACA CTATAAAGGA
    TTTTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287076.1
    CDS524..2812
    Translation

    Target ORF information:

    RefSeq Version NM_001300147.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant R, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300147.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GGAACTACAG CTGTCTTAGG
    GTCGATTTAA TCTTTACCAG AGATCGTGCA TTCTATTTCA CCACCGTTTT TATACCCGGC
    ATTATATTGG TGACGTCGTC CTTTATAACT TTCTGGCTAG AATGGAATGC CGTGCCAGCT
    AGATCGATGA TAGGCGTGAC AACAATGCTG AATTTCTTCA CTACCTCGAA CGGTTTCCGG
    AGCACTCTGC CGGTTGTTTC TAATCTGACG GCGATGAACG TGTGGGACGG CGTGTGTATG
    TGCTTCATCT ACGCCTCCTT GCTGGAATTC GTCTGCGTCA ATTATGTGGG GCGAAAGCGA
    CCCCTGCACA ACGTCGTCTA TCGGCCCGGC GAGAATCCCG TAACACAGCA GGGCGAAAAG
    CGTCAAAACG CCGGGGCACC GAACGAAATT GTGGCATGCA CCACCTGCGG CGGCAGCAAC
    AGTCCGTGCA CCCATTCGGC AAATAATGGA TGTGCCACGG AGACCTGCTT CGTTCAAGTG
    CGGAAAAAGG AGCCACCTCA TCCGATTCGA GTGGCCAAGA CGATCGATGT CATCGCTAGA
    ATTACATTTC CAACTGCTTA TGCCATTTTT CTGATATTCT TCTTTGTACA CTATAAAGGA
    TTTTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa87770
    Clone ID Related Accession (Same CDS sequence) NM_001370024.1
    Accession Version NM_001370024.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform S
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGATG AAGACTTTTG CTGTGGCTTG
    CAATCGCCCG ATATGGCAAC TAATCAAAAT GCACGGAGAC CACACAATCG CGGCACTCTG
    ACGGTGAACG TGAACGTGCT GCTCCTGAGC TTAGCATCGC CCGACGAAAG CAGTTTGAAA
    TACGAGGTTG AATTTCTGCT GAACCAGCAA TGGAACGATC CGCGACTGCA ATATGGCAAT
    AAGTCTCATT ATGACTTCTT AAATGCTCTG CATCATCATG ATAGCATCTG GACGCCGGAT
    ACGTATTTCA TTATGCATGG CGATTTCAAG GATCCCATCA TACCAATGCA TTTCGCTTTA
    AGAATATTTC GGAACGGGAC CATTACGTAC GCAATGAGAC GTCACCTGAT CTTATCCTGC
    CAGGGAAGTC TTCATATATT TCCATTCGAT GACCCCAAGT GTTCCTTCTC CATGGAGAGC
    ATTTCCTACG AGGAGGCCCA AATCAAGTAC GTCTGGAAAA ACGATGAGGA CACTCTGCGG
    AAGAGTCCTT CGCTGACCAC ATTGAACGCG TACTTAATCA AGAATCAAAC GACGGCCTGC
    GATCAGAACA GCTGGAGAGG TAATTACAGT TGCCTACAGG TCGAGTTGAC ATTTACCCGT
    GATCGTGCGT ATTATTTTAC AACCGTTTTC ATACCTGGCA TTATATTGGT CACCTCGTCG
    TTTATCACAT TTTGGCTGGA ATGGAATGCA GTCCCAGCCC GGGTTATGAT CGGCGTGACA
    ACAATGCTGA ATTTCTTCAC TACCTCGAAC GGTTTCCGGA GCACTCTGCC GGTTGTTTCT
    AATCTGACGG CGATGAACGT GTGGGACGGC GTGTGTATGT GCTTCATCTA CGCCTCCTTG
    CTGGAATTCG TCTGCGTCAA TTATGTGGGG CGAAAGCGAC CCCTGCACAA CGTCGTCTAT
    CGGCCCGGCG AGAATCCCGT AACACAGCGT CTTCCAGCAG TACTAAGCAG GATCGGAGTA
    ATTCTTGCAA GTCCTTTGCC CGGCGAAAAG CGTCAAAACG CCGGGGCACC GAACGAAATT
    GTGGCATGCA CCACCTGCGG CGGCAGCAAC AGTCCGTGCA CCCATTCGGC AAATAATGGA
    TGTGCCACGG AGACCTGCTT CGTTCAAGTG CGGAAAAAGG AGCCACCTCA TCCGATTCGA
    GTGGCCAAGA CGATCGATGT CATCGCTAGA ATTACATTTC CAACTGCTTA TGCCATTTTT
    CTGATATTCT TCTTTGTACA CTATAAAGGA TTTTCGTAAA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001356981.1
    CDS524..2848
    Translation

    Target ORF information:

    RefSeq Version NM_001370024.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant S, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001370024.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGATG AAGACTTTTG CTGTGGCTTG
    CAATCGCCCG ATATGGCAAC TAATCAAAAT GCACGGAGAC CACACAATCG CGGCACTCTG
    ACGGTGAACG TGAACGTGCT GCTCCTGAGC TTAGCATCGC CCGACGAAAG CAGTTTGAAA
    TACGAGGTTG AATTTCTGCT GAACCAGCAA TGGAACGATC CGCGACTGCA ATATGGCAAT
    AAGTCTCATT ATGACTTCTT AAATGCTCTG CATCATCATG ATAGCATCTG GACGCCGGAT
    ACGTATTTCA TTATGCATGG CGATTTCAAG GATCCCATCA TACCAATGCA TTTCGCTTTA
    AGAATATTTC GGAACGGGAC CATTACGTAC GCAATGAGAC GTCACCTGAT CTTATCCTGC
    CAGGGAAGTC TTCATATATT TCCATTCGAT GACCCCAAGT GTTCCTTCTC CATGGAGAGC
    ATTTCCTACG AGGAGGCCCA AATCAAGTAC GTCTGGAAAA ACGATGAGGA CACTCTGCGG
    AAGAGTCCTT CGCTGACCAC ATTGAACGCG TACTTAATCA AGAATCAAAC GACGGCCTGC
    GATCAGAACA GCTGGAGAGG TAATTACAGT TGCCTACAGG TCGAGTTGAC ATTTACCCGT
    GATCGTGCGT ATTATTTTAC AACCGTTTTC ATACCTGGCA TTATATTGGT CACCTCGTCG
    TTTATCACAT TTTGGCTGGA ATGGAATGCA GTCCCAGCCC GGGTTATGAT CGGCGTGACA
    ACAATGCTGA ATTTCTTCAC TACCTCGAAC GGTTTCCGGA GCACTCTGCC GGTTGTTTCT
    AATCTGACGG CGATGAACGT GTGGGACGGC GTGTGTATGT GCTTCATCTA CGCCTCCTTG
    CTGGAATTCG TCTGCGTCAA TTATGTGGGG CGAAAGCGAC CCCTGCACAA CGTCGTCTAT
    CGGCCCGGCG AGAATCCCGT AACACAGCGT CTTCCAGCAG TACTAAGCAG GATCGGAGTA
    ATTCTTGCAA GTCCTTTGCC CGGCGAAAAG CGTCAAAACG CCGGGGCACC GAACGAAATT
    GTGGCATGCA CCACCTGCGG CGGCAGCAAC AGTCCGTGCA CCCATTCGGC AAATAATGGA
    TGTGCCACGG AGACCTGCTT CGTTCAAGTG CGGAAAAAGG AGCCACCTCA TCCGATTCGA
    GTGGCCAAGA CGATCGATGT CATCGCTAGA ATTACATTTC CAACTGCTTA TGCCATTTTT
    CTGATATTCT TCTTTGTACA CTATAAAGGA TTTTCGTAAA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa87771
    Clone ID Related Accession (Same CDS sequence) NM_001370025.1
    Accession Version NM_001370025.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform T
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GGAACTACAG CTGTCTTAGG
    GTCGATTTAA TCTTTACCAG AGATCGTGCA TTCTATTTCA CCACCGTTTT TATACCCGGC
    ATTATATTGG TGACGTCGTC CTTTATAACT TTCTGGCTAG AATGGAATGC CGTGCCAGCT
    AGATCGATGA TAGGCGTGAC AACAATGCTG AATTTCTTCA CTACCTCGAA CGGTTTCCGG
    AGCACTCTGC CGGTTGTTTC TAATCTGACG GCGATGAACG TGTGGGACGG CGTGTGTATG
    TGCTTCATCT ACGCCTCCTT GCTGGAATTC GTCTGCGTCA ATTATGTGGG GCGAAAGCGA
    CCCCTGCACA ACGTCGTCTA TCGGCCCGGC GAGAATCCCG TAACACAGTT TTTTAAATTT
    ACCGCCGTCT ATCAGGGCGA AAAGCGTCAA AACGCCGGGG CACCGAACGA AATTGTGGCA
    TGCACCACCT GCGGCGGCAG CAACAGTCCG TGCACCCATT CGGCAAATAA TGGATGTGCC
    ACGGAGACCT GCTTCGTTCA AGTGCGGAAA AAGGAGCCAC CTCATCCGAT TCGAGTGGCC
    AAGACGATCG ATGTCATCGC TAGAATTACA TTTCCAACTG CTTATGCCAT TTTTCTGATA
    TTCTTCTTTG TACACTATAA AGGATTTTCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001356910.1
    CDS524..2836
    Translation

    Target ORF information:

    RefSeq Version NM_001370025.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant T, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001370025.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATTC ATGGCATCGA TTGGTCGAAA TTTGATGAGT CCAGTTCGGA TAAGGAAATA
    TTAGATTTAC TGCTTGAGAA GAAGCGCTAC GATAAACGAT TACTGCCGCC TGTAAATGAT
    GAAGACTTTT GCTGTGGCTT GCAATCGCCC GATATGGCAA CTAATCAAAA TGCACGGAGA
    CCACACAATC GCGGCACTCT GACGGTGAAC GTGAACGTGC TGCTCCTGAG CTTAGCATCG
    CCCGACGAAA GCAGTTTGAA ATACGAGGTT GAATTTCTGC TGAACCAGCA ATGGAACGAT
    CCGCGACTGC AATATGGCAA TAAGTCTCAT TATGACTTCT TAAATGCTCT GCATCATCAT
    GATAGCATCT GGACGCCGGA TACGTATTTC ATTATGCATG GCGATTTCAA GGATCCCATC
    ATACCAATGC ATTTCGCTTT AAGAATATTT CGGAACGGGA CCATTACGTA CGCAATGAGA
    CGTCACCTGA TCTTATCCTG CCAGGGAAGT CTTCATATAT TTCCATTCGA TGACCCCAAG
    TGTTCCTTCT CCATGGAGAG CATTTCCTAC GAGGAGGCCC AAATCAAGTA CGTCTGGAAA
    AACGATGAGG ACACTCTGCG GAAGAGTCCT TCGCTGACCA CATTGAACGC GTACTTAATC
    AAGAATCAAA CGACGGCCTG CGATCAGAAC AGCTGGAGAG GGAACTACAG CTGTCTTAGG
    GTCGATTTAA TCTTTACCAG AGATCGTGCA TTCTATTTCA CCACCGTTTT TATACCCGGC
    ATTATATTGG TGACGTCGTC CTTTATAACT TTCTGGCTAG AATGGAATGC CGTGCCAGCT
    AGATCGATGA TAGGCGTGAC AACAATGCTG AATTTCTTCA CTACCTCGAA CGGTTTCCGG
    AGCACTCTGC CGGTTGTTTC TAATCTGACG GCGATGAACG TGTGGGACGG CGTGTGTATG
    TGCTTCATCT ACGCCTCCTT GCTGGAATTC GTCTGCGTCA ATTATGTGGG GCGAAAGCGA
    CCCCTGCACA ACGTCGTCTA TCGGCCCGGC GAGAATCCCG TAACACAGTT TTTTAAATTT
    ACCGCCGTCT ATCAGGGCGA AAAGCGTCAA AACGCCGGGG CACCGAACGA AATTGTGGCA
    TGCACCACCT GCGGCGGCAG CAACAGTCCG TGCACCCATT CGGCAAATAA TGGATGTGCC
    ACGGAGACCT GCTTCGTTCA AGTGCGGAAA AAGGAGCCAC CTCATCCGAT TCGAGTGGCC
    AAGACGATCG ATGTCATCGC TAGAATTACA TTTCCAACTG CTTATGCCAT TTTTCTGATA
    TTCTTCTTTG TACACTATAA AGGATTTTCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa87772
    Clone ID Related Accession (Same CDS sequence) NM_001370026.1
    Accession Version NM_001370026.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2268bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pH-sensitive chloride channel 1, isoform U
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGGCA CTCTGACGGT GAACGTGAAC
    GTGCTGCTCC TGAGCTTAGC ATCGCCCGAC GAAAGCAGTT TGAAATACGA GGTTGAATTT
    CTGCTGAACC AGCAATGGAA CGATCCGCGA CTGCAATATG GCAATAAGTC TCATTATGAC
    TTCTTAAATG CTCTGCATCA TCATGATAGC ATCTGGACGC CGGATACGTA TTTCATTATG
    CATGGCGATT TCAAGGATCC CATCATACCA ATGCATTTCG CTTTAAGAAT ATTTCGGAAC
    GGGACCATTA CGTACGCAAT GAGACGTCAC CTGATCTTAT CCTGCCAGGG AAGTCTTCAT
    ATATTTCCAT TCGATGACCC CAAGTGTTCC TTCTCCATGG AGAGCATTTC CTACGAGGAG
    GCCCAAATCA AGTACGTCTG GAAAAACGAT GAGGACACTC TGCGGAAGAG TCCTTCGCTG
    ACCACATTGA ACGCGTACTT AATCAAGAAT CAAACGACGG CCTGCGATCA GAACAGCTGG
    AGAGGTAATT ACAGTTGCCT ACAGGTCGAG TTGACATTTA CCCGTGATCG TGCGTATTAT
    TTTACAACCG TTTTCATACC TGGCATTATA TTGGTCACCT CGTCGTTTAT CACATTTTGG
    CTGGAATGGA ATGCAGTCCC AGCCCGGGTT ATGATCGGCG TGACAACAAT GCTGAATTTC
    TTCACTACCT CGAACGGTTT CCGGAGCACT CTGCCGGTTG TTTCTAATCT GACGGCGATG
    AACGTGTGGG ACGGCGTGTG TATGTGCTTC ATCTACGCCT CCTTGCTGGA ATTCGTCTGC
    GTCAATTATG TGGGGCGAAA GCGACCCCTG CACAACGTCG TCTATCGGCC CGGCGAGAAT
    CCCGTAACAC AGCGTCTTCC AGCAGTACTA AGCAGGATCG GAGTAATTCT TGCAAGTCCT
    TTGTTTTTTA AATTTACCGC CGTCTATCAG GGCGAAAAGC GTCAAAACGC CGGGGCACCG
    AACGAAATTG TGGCATGCAC CACCTGCGGC GGCAGCAACA GTCCGTGCAC CCATTCGGCA
    AATAATGGAT GTGCCACGGA GACCTGCTTC GTTCAAGTGC GGAAAAAGGA GCCACCTCAT
    CCGATTCGAG TGGCCAAGAC GATCGATGTC ATCGCTAGAA TTACATTTCC AACTGCTTAT
    GCCATTTTTC TGATATTCTT CTTTGTACAC TATAAAGGAT TTTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001356909.1
    CDS524..2791
    Translation

    Target ORF information:

    RefSeq Version NM_001370026.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pH-sensitive chloride channel 1 (pHCl-1), transcript variant U, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001370026.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCTGCG TCTTTGAAGC GGCCAAAGAG CAGCCCCACA AACCGAGAAG CAGGCAACTG 
    AGGCGCAACC AATGCCGTGG AAAAGCGGAA AAAGCAGCAG CGGGGACCAG AGGCAAGGTC
    GCTGTCAGAG GAAACAACGA CAAGCCAGCT GAGGACATCA AAATCAAGGA TAACTATCAA
    CAAAACCATA AAACGCAAAC CGAAACACAA ACAGAAGTAG CAGCAGTAGT AGCAATAGCA
    ACAGACGCAG GAGCAGAAAC AGAAACTGAA ACAGAGGCTG AGGCCCAAAA AGAGCCAGCA
    ACCGGGCCCG AACATCTGAA GGCGGCGGCT CAAGTGCTTT TAACGATACG GCAGGCACGT
    CAGGAGAACA ACAAGCACAG TAGTAGTCAA GTGGCTGAAA CGCACAAGGA CGAAGGCGAG
    GACGCGTTGG CGCGGCAAGG ATCCATTGTC GATACGGCGG CGGGCAGAAT CGGAATCGAT
    ATTGCGGCAA CAACGATTCA AGTCGCCGAG CGGCATGAGG ATGGGCAGCA TTGGCAGCAA
    CACAATGTCC GCCGGCGGAG GCGCCAGCCA GATCCTGCAG CGCATCCTGC AAGCATCACC
    AAACTTTCGG CAATATCCCT GCATTTTCAC GCCCTCGCCG CCCTAGCGAA AACGCTCCAA
    AAGCACGAGG ACCTGAGTCC TGTCCAGGAT CGCCAGCCCC TAGCGTACCG GGCGTGGCAA
    GAACATCCGT ATGCCGAGAC CCAAATGCCC GCCACCATGT CAGCGGCCAT CATTGCGAAT
    GTCGGCGCTA CTGCAGCGGC CACCTCGGCA GCCGCCTTCC AGTATCTCGG CCAGCACTAC
    AAGCACTACA GCCAGTCGAT CAGCTTCTAC GCCGCCTCCA GTGTGCTCCT GATGCTTTGT
    TATCTGACCA CCGCGTCCAC GGCAGCTGCC ACGCAGTCGG AGACGGGTGC CCTCGACAAG
    CATCCCATAT TTGATGAGTC CAGTTCGGAT AAGGAAATAT TAGATTTACT GCTTGAGAAG
    AAGCGCTACG ATAAACGATT ACTGCCGCCT GTAAATGGCA CTCTGACGGT GAACGTGAAC
    GTGCTGCTCC TGAGCTTAGC ATCGCCCGAC GAAAGCAGTT TGAAATACGA GGTTGAATTT
    CTGCTGAACC AGCAATGGAA CGATCCGCGA CTGCAATATG GCAATAAGTC TCATTATGAC
    TTCTTAAATG CTCTGCATCA TCATGATAGC ATCTGGACGC CGGATACGTA TTTCATTATG
    CATGGCGATT TCAAGGATCC CATCATACCA ATGCATTTCG CTTTAAGAAT ATTTCGGAAC
    GGGACCATTA CGTACGCAAT GAGACGTCAC CTGATCTTAT CCTGCCAGGG AAGTCTTCAT
    ATATTTCCAT TCGATGACCC CAAGTGTTCC TTCTCCATGG AGAGCATTTC CTACGAGGAG
    GCCCAAATCA AGTACGTCTG GAAAAACGAT GAGGACACTC TGCGGAAGAG TCCTTCGCTG
    ACCACATTGA ACGCGTACTT AATCAAGAAT CAAACGACGG CCTGCGATCA GAACAGCTGG
    AGAGGTAATT ACAGTTGCCT ACAGGTCGAG TTGACATTTA CCCGTGATCG TGCGTATTAT
    TTTACAACCG TTTTCATACC TGGCATTATA TTGGTCACCT CGTCGTTTAT CACATTTTGG
    CTGGAATGGA ATGCAGTCCC AGCCCGGGTT ATGATCGGCG TGACAACAAT GCTGAATTTC
    TTCACTACCT CGAACGGTTT CCGGAGCACT CTGCCGGTTG TTTCTAATCT GACGGCGATG
    AACGTGTGGG ACGGCGTGTG TATGTGCTTC ATCTACGCCT CCTTGCTGGA ATTCGTCTGC
    GTCAATTATG TGGGGCGAAA GCGACCCCTG CACAACGTCG TCTATCGGCC CGGCGAGAAT
    CCCGTAACAC AGCGTCTTCC AGCAGTACTA AGCAGGATCG GAGTAATTCT TGCAAGTCCT
    TTGTTTTTTA AATTTACCGC CGTCTATCAG GGCGAAAAGC GTCAAAACGC CGGGGCACCG
    AACGAAATTG TGGCATGCAC CACCTGCGGC GGCAGCAACA GTCCGTGCAC CCATTCGGCA
    AATAATGGAT GTGCCACGGA GACCTGCTTC GTTCAAGTGC GGAAAAAGGA GCCACCTCAT
    CCGATTCGAG TGGCCAAGAC GATCGATGTC ATCGCTAGAA TTACATTTCC AACTGCTTAT
    GCCATTTTTC TGATATTCTT CTTTGTACAC TATAAAGGAT TTTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.