RhoGAP71E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RhoGAP71E
Entrez Gene ID	39693
Full Name	Rho GTPase activating protein at 71E
Synonyms	BcDNA:LD07615,CG16980,CG32149,CG7396,Dmel\CG32149,RhoGAP-71E1,RhoGap71E,l(3)j6B9
General protein information	Preferred Names Rho GTPase activating protein at 71E Names CG32149 gene product from transcript CG32149-RD CG32149-PA CG32149-PB CG32149-PC CG32149-PD CG32149-PE CG32149-PF CG32149-PG RhoGAP71E-PA RhoGAP71E-PB RhoGAP71E-PC RhoGAP71E-PD RhoGAP71E-PE RhoGAP71E-PF RhoGAP71E-PG
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 71E1-71E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274957.1	NP_001261886.1	Rho GTPase activating protein at 71E, isoform F
NM_001202183.2	NP_001189112.1	Rho GTPase activating protein at 71E, isoform E
NM_168630.4	NP_730061.1	Rho GTPase activating protein at 71E, isoform B
NM_140527.5	NP_648784.1	Rho GTPase activating protein at 71E, isoform A
NM_168629.4	NP_730060.1	Rho GTPase activating protein at 71E, isoform C
NM_001144484.3	NP_001137956.1	Rho GTPase activating protein at 71E, isoform D
NM_001274958.1	NP_001261887.1	Rho GTPase activating protein at 71E, isoform G

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011111	XP_309534.3
Caenorhabditis elegans (roundworm)	CELE_2RSSE.1	NP_872058.1
Drosophila melanogaster (fruit fly)	RhoGAP71E	NP_001137956.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RhoGAP71E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RhoGAP71E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18567	NM_140527.5 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa18568	NM_168630.4 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa20041	NM_001202183.2 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa20042	NM_001274957.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa20043	NM_001274958.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18565	NM_001144484.3 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa18566	NM_168629.4 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RhoGAP71E ( NM_140527.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648784.1 Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18567
Clone ID Related Accession (Same CDS sequence)	NM_140527.5
Accession Version	NM_140527.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 71E, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140527.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGCT GGGCAGAACG CGTCCATAGG CGCGCCGCGG ATTTGGGCAA CGTGGTGACC 
TCCTGCGGCC ACCCAGCCAC CGCTCCCGCC TACCCGTACC GCCTCGAAAA GGTCAAATTC 
GGTGTGCCGC TGGAGGAGGT GTGCAAGCAC AACAACAACA TTCCCGGGCC CCTGCTCGTC 
CTGATCCTCA AACTGAACAA GGAGTCACCG AACCGCCGCG ATGTATTCCG CGCACCCGGC 
CACCAGGGCG CAATGAAGAA GCTCATCCAC TTCCTGCAGG CCGGTCGGCT CGTCAACGTG 
GACAACTATT CGGTGTTCAC CATCGCCAGC GTGCTCAAGA AGTTCCTGCG CAAGATCCCC 
AATGGGATAT TCGGGCGCAG CGGCGAGATG GAGCTGTTCG CCATCAATGA TCTGCAAAAC 
GAAGCCGAGC AAACGGAGCG ACTGCACAGA CTCTTCGGTT CGCTGCCGAA ATACACACAG 
CATCTATTAG TTCTACTGTT CGGCACATTC CGGGTTATCG CGAGCAATGC GGCCCACGCC 
TCCACCGGAA TGACCAGCGA GGCGCTGGGC GTTTCCGTGG CCCCCAGTTT CTTTCAATCC 
TGCGTCAGCG ATGGCAAAAC CGCCCGCATG GAGGATGTGC TCAAGTTCAA GGTCTCTACG 
AAGATTATGC AGAATATAAT TGATAAGTTT GCCACGCACG ACATCTTCGG GCGCGACAAC 
TATGAGTACT ACGCCCGCGT CACAGGACGC ATTCTCAAGG TGCAGGACGA GTGGATATGC 
AGCTTTCAGT ATCCACCACC GCCACGTGGC AAGTTGGCTC AACAAAAATA CGCATTGCAA 
CATTCTTTGG AGGCGGAGAA GACATGGCTG CAGTGCCAAT GCGAGCGTTG GGGCTTACTG 
GCCCAGGAGG AGTCCCGCTC GACGCCCGCC CTGCTGACCA GCTCCAGTTC CGCATTGGAG 
AACAGTGTCC TGTCGCTGGG CGTTTCCCCC GAACACTCGT TCATCGAGAG CTGCGCCCGT 
CTCTCAGTTT CGCTGGAGGG ACCTGGCATC TATGCCATGA CTTCGTCGCC GTTGCCGGCG 
AAGCGGAATG GCAATGGCAA CGGAAATGCC ACGGACTACG ACTATGATGA CTATGCCGAC 
GATGATGAGG ACAACGATGA GCTGGACGGC GTTCACGACT TTGGTGCCGA ATTGGAGATC 
AGTAGCATAG CTCCGCCGCA GGGAAGTCGT GGCATCTATC GCCACAAGCA GCGAGCCCAG 
CAGCAGCCAC ACCACTACCA ACAACAGCAG CAGCAGCAGC AGGTGCCGCC TCAGCACCAG 
CGGGACTACG ACGAGGACGA CGACGATGAT GAGGATGAGA TGACGTTTAT TAAGCGCCGG 
TATCGACAGA ACAAAAAGAA GCCGGCGCCG CCGAGCAGCA GCCATCATCA GAGGCGATTG 
CGCACTGGCA CCAAGAGTCT CGATGGCGGA GGGGAGAGGC GCACGGGATC GGGAGCAGCG 
GGCGGATCAG GAGGAGGTGC TTCAGGCTCG GCAGTTGGCA ATCCGACGCC TCCCAAGCTC 
AAGCAGCGAA CGTGCAGCCG CTGCAATCGA GGCATTCGGC ACAGTAGCTC GTGCAGCTCC 
AATTCCGCCG GAGCAACTGG CTCGAATAGC GGAGGAGGCG GCAACGGAGG CTCCGGAGCC 
GGAAACGGAG GAGGGGCCGA GGGCGGCATG ACCATGGAGG AGCTGAGGGC CGTCAACCGA 
TATGCGGAGA GCACCAAGAG CCTCTCGTAT CTGCCACAGG TGAGGGACGA TTTCTTGCTG 
GGCTCACGCA TTTCCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648784.1
CDS	689..2509
Translation	MSSWAERVHRRAADLGNVVTSCGHPATAPAYPYRLEKVKFGVPLEEVCKHNNNIPGPLLVLILKLNKESPNRRDVFRAPGHQGAMKKLIHFLQAGRLVNVDNYSVFTIASVLKKFLRKIPNGIFGRSGEMELFAINDLQNEAEQTERLHRLFGSLPKYTQHLLVLLFGTFRVIASNAAHASTGMTSEALGVSVAPSFFQSCVSDGKTARMEDVLKFKVSTKIMQNIIDKFATHDIFGRDNYEYYARVTGRILKVQDEWICSFQYPPPPRGKLAQQKYALQHSLEAEKTWLQCQCERWGLLAQEESRSTPALLTSSSSALENSVLSLGVSPEHSFIESCARLSVSLEGPGIYAMTSSPLPAKRNGNGNGNATDYDYDDYADDDEDNDELDGVHDFGAELEISSIAPPQGSRGIYRHKQRAQQQPHHYQQQQQQQQVPPQHQRDYDEDDDDDEDEMTFIKRRYRQNKKKPAPPSSSHHQRRLRTGTKSLDGGGERRTGSGAAGGSGGGASGSAVGNPTPPKLKQRTCSRCNRGIRHSSSCSSNSAGATGSNSGGGGNGGSGAGNGGGAEGGMTMEELRAVNRYAESTKSLSYLPQVRDDFLLGSRISV

Target ORF information:

RefSeq Version	NM_140527.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140527.5

ORF Insert Sequence:

ATGTCCAGCT GGGCAGAACG CGTCCATAGG CGCGCCGCGG ATTTGGGCAA CGTGGTGACC 
TCCTGCGGCC ACCCAGCCAC CGCTCCCGCC TACCCGTACC GCCTCGAAAA GGTCAAATTC 
GGTGTGCCGC TGGAGGAGGT GTGCAAGCAC AACAACAACA TTCCCGGGCC CCTGCTCGTC 
CTGATCCTCA AACTGAACAA GGAGTCACCG AACCGCCGCG ATGTATTCCG CGCACCCGGC 
CACCAGGGCG CAATGAAGAA GCTCATCCAC TTCCTGCAGG CCGGTCGGCT CGTCAACGTG 
GACAACTATT CGGTGTTCAC CATCGCCAGC GTGCTCAAGA AGTTCCTGCG CAAGATCCCC 
AATGGGATAT TCGGGCGCAG CGGCGAGATG GAGCTGTTCG CCATCAATGA TCTGCAAAAC 
GAAGCCGAGC AAACGGAGCG ACTGCACAGA CTCTTCGGTT CGCTGCCGAA ATACACACAG 
CATCTATTAG TTCTACTGTT CGGCACATTC CGGGTTATCG CGAGCAATGC GGCCCACGCC 
TCCACCGGAA TGACCAGCGA GGCGCTGGGC GTTTCCGTGG CCCCCAGTTT CTTTCAATCC 
TGCGTCAGCG ATGGCAAAAC CGCCCGCATG GAGGATGTGC TCAAGTTCAA GGTCTCTACG 
AAGATTATGC AGAATATAAT TGATAAGTTT GCCACGCACG ACATCTTCGG GCGCGACAAC 
TATGAGTACT ACGCCCGCGT CACAGGACGC ATTCTCAAGG TGCAGGACGA GTGGATATGC 
AGCTTTCAGT ATCCACCACC GCCACGTGGC AAGTTGGCTC AACAAAAATA CGCATTGCAA 
CATTCTTTGG AGGCGGAGAA GACATGGCTG CAGTGCCAAT GCGAGCGTTG GGGCTTACTG 
GCCCAGGAGG AGTCCCGCTC GACGCCCGCC CTGCTGACCA GCTCCAGTTC CGCATTGGAG 
AACAGTGTCC TGTCGCTGGG CGTTTCCCCC GAACACTCGT TCATCGAGAG CTGCGCCCGT 
CTCTCAGTTT CGCTGGAGGG ACCTGGCATC TATGCCATGA CTTCGTCGCC GTTGCCGGCG 
AAGCGGAATG GCAATGGCAA CGGAAATGCC ACGGACTACG ACTATGATGA CTATGCCGAC 
GATGATGAGG ACAACGATGA GCTGGACGGC GTTCACGACT TTGGTGCCGA ATTGGAGATC 
AGTAGCATAG CTCCGCCGCA GGGAAGTCGT GGCATCTATC GCCACAAGCA GCGAGCCCAG 
CAGCAGCCAC ACCACTACCA ACAACAGCAG CAGCAGCAGC AGGTGCCGCC TCAGCACCAG 
CGGGACTACG ACGAGGACGA CGACGATGAT GAGGATGAGA TGACGTTTAT TAAGCGCCGG 
TATCGACAGA ACAAAAAGAA GCCGGCGCCG CCGAGCAGCA GCCATCATCA GAGGCGATTG 
CGCACTGGCA CCAAGAGTCT CGATGGCGGA GGGGAGAGGC GCACGGGATC GGGAGCAGCG 
GGCGGATCAG GAGGAGGTGC TTCAGGCTCG GCAGTTGGCA ATCCGACGCC TCCCAAGCTC 
AAGCAGCGAA CGTGCAGCCG CTGCAATCGA GGCATTCGGC ACAGTAGCTC GTGCAGCTCC 
AATTCCGCCG GAGCAACTGG CTCGAATAGC GGAGGAGGCG GCAACGGAGG CTCCGGAGCC 
GGAAACGGAG GAGGGGCCGA GGGCGGCATG ACCATGGAGG AGCTGAGGGC CGTCAACCGA 
TATGCGGAGA GCACCAAGAG CCTCTCGTAT CTGCCACAGG TGAGGGACGA TTTCTTGCTG 
GGCTCACGCA TTTCCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP71E ( NM_168630.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730061.1 Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18568
Clone ID Related Accession (Same CDS sequence)	NM_168630.4
Accession Version	NM_168630.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 71E, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168630.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGCT GGGCAGAACG CGTCCATAGG CGCGCCGCGG ATTTGGGCAA CGTGGTGACC 
TCCTGCGGCC ACCCAGCCAC CGCTCCCGCC TACCCGTACC GCCTCGAAAA GGTCAAATTC 
GGTGTGCCGC TGGAGGAGGT GTGCAAGCAC AACAACAACA TTCCCGGGCC CCTGCTCGTC 
CTGATCCTCA AACTGAACAA GGAGTCACCG AACCGCCGCG ATGTATTCCG CGCACCCGGC 
CACCAGGGCG CAATGAAGAA GCTCATCCAC TTCCTGCAGG CCGGTCGGCT CGTCAACGTG 
GACAACTATT CGGTGTTCAC CATCGCCAGC GTGCTCAAGA AGTTCCTGCG CAAGATCCCC 
AATGGGATAT TCGGGCGCAG CGGCGAGATG GAGCTGTTCG CCATCAATGA TCTGCAAAAC 
GAAGCCGAGC AAACGGAGCG ACTGCACAGA CTCTTCGGTT CGCTGCCGAA ATACACACAG 
CATCTATTAG TTCTACTGTT CGGCACATTC CGGGTTATCG CGAGCAATGC GGCCCACGCC 
TCCACCGGAA TGACCAGCGA GGCGCTGGGC GTTTCCGTGG CCCCCAGTTT CTTTCAATCC 
TGCGTCAGCG ATGGCAAAAC CGCCCGCATG GAGGATGTGC TCAAGTTCAA GGTCTCTACG 
AAGATTATGC AGAATATAAT TGATAAGTTT GCCACGCACG ACATCTTCGG GCGCGACAAC 
TATGAGTACT ACGCCCGCGT CACAGGACGC ATTCTCAAGG TGCAGGACGA GTGGATATGC 
AGCTTTCAGT ATCCACCACC GCCACGTGGC AAGTTGGCTC AACAAAAATA CGCATTGCAA 
CATTCTTTGG AGGCGGAGAA GACATGGCTG CAGTGCCAAT GCGAGCGTTG GGGCTTACTG 
GCCCAGGAGG AGTCCCGCTC GACGCCCGCC CTGCTGACCA GCTCCAGTTC CGCATTGGAG 
AACAGTGTCC TGTCGCTGGG CGTTTCCCCC GAACACTCGT TCATCGAGAG CTGCGCCCGT 
CTCTCAGTTT CGCTGGAGGG ACCTGGCATC TATGCCATGA CTTCGTCGCC GTTGCCGGCG 
AAGCGGAATG GCAATGGCAA CGGAAATGCC ACGGACTACG ACTATGATGA CTATGCCGAC 
GATGATGAGG ACAACGATGA GCTGGACGGC GTTCACGACT TTGGTGCCGA ATTGGAGATC 
AGTAGCATAG CTCCGCCGCA GGGAAGTCGT GGCATCTATC GCCACAAGCA GCGAGCCCAG 
CAGCAGCCAC ACCACTACCA ACAACAGCAG CAGCAGCAGC AGGTGCCGCC TCAGCACCAG 
CGGGACTACG ACGAGGACGA CGACGATGAT GAGGATGAGA TGACGTTTAT TAAGCGCCGG 
TATCGACAGA ACAAAAAGAA GCCGGCGCCG CCGAGCAGCA GCCATCATCA GAGGCGATTG 
CGCACTGGCA CCAAGAGTCT CGATGGCGGA GGGGAGAGGC GCACGGGATC GGGAGCAGCG 
GGCGGATCAG GAGGAGGTGC TTCAGGCTCG GCAGTTGGCA ATCCGACGCC TCCCAAGCTC 
AAGCAGCGAA CGTGCAGCCG CTGCAATCGA GGCATTCGGC ACAGTAGCTC GTGCAGCTCC 
AATTCCGCCG GAGCAACTGG CTCGAATAGC GGAGGAGGCG GCAACGGAGG CTCCGGAGCC 
GGAAACGGAG GAGGGGCCGA GGGCGGCATG ACCATGGAGG AGCTGAGGGC CGTCAACCGA 
TATGCGGAGA GCACCAAGAG CCTCTCGTAT CTGCCACAGG AAATGAAAAA GAATCAAGAA 
CCAAGAATAT ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730061.1
CDS	689..2503
Translation	MSSWAERVHRRAADLGNVVTSCGHPATAPAYPYRLEKVKFGVPLEEVCKHNNNIPGPLLVLILKLNKESPNRRDVFRAPGHQGAMKKLIHFLQAGRLVNVDNYSVFTIASVLKKFLRKIPNGIFGRSGEMELFAINDLQNEAEQTERLHRLFGSLPKYTQHLLVLLFGTFRVIASNAAHASTGMTSEALGVSVAPSFFQSCVSDGKTARMEDVLKFKVSTKIMQNIIDKFATHDIFGRDNYEYYARVTGRILKVQDEWICSFQYPPPPRGKLAQQKYALQHSLEAEKTWLQCQCERWGLLAQEESRSTPALLTSSSSALENSVLSLGVSPEHSFIESCARLSVSLEGPGIYAMTSSPLPAKRNGNGNGNATDYDYDDYADDDEDNDELDGVHDFGAELEISSIAPPQGSRGIYRHKQRAQQQPHHYQQQQQQQQVPPQHQRDYDEDDDDDEDEMTFIKRRYRQNKKKPAPPSSSHHQRRLRTGTKSLDGGGERRTGSGAAGGSGGGASGSAVGNPTPPKLKQRTCSRCNRGIRHSSSCSSNSAGATGSNSGGGGNGGSGAGNGGGAEGGMTMEELRAVNRYAESTKSLSYLPQEMKKNQEPRIY

Target ORF information:

RefSeq Version	NM_168630.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168630.4

ORF Insert Sequence:

ATGTCCAGCT GGGCAGAACG CGTCCATAGG CGCGCCGCGG ATTTGGGCAA CGTGGTGACC 
TCCTGCGGCC ACCCAGCCAC CGCTCCCGCC TACCCGTACC GCCTCGAAAA GGTCAAATTC 
GGTGTGCCGC TGGAGGAGGT GTGCAAGCAC AACAACAACA TTCCCGGGCC CCTGCTCGTC 
CTGATCCTCA AACTGAACAA GGAGTCACCG AACCGCCGCG ATGTATTCCG CGCACCCGGC 
CACCAGGGCG CAATGAAGAA GCTCATCCAC TTCCTGCAGG CCGGTCGGCT CGTCAACGTG 
GACAACTATT CGGTGTTCAC CATCGCCAGC GTGCTCAAGA AGTTCCTGCG CAAGATCCCC 
AATGGGATAT TCGGGCGCAG CGGCGAGATG GAGCTGTTCG CCATCAATGA TCTGCAAAAC 
GAAGCCGAGC AAACGGAGCG ACTGCACAGA CTCTTCGGTT CGCTGCCGAA ATACACACAG 
CATCTATTAG TTCTACTGTT CGGCACATTC CGGGTTATCG CGAGCAATGC GGCCCACGCC 
TCCACCGGAA TGACCAGCGA GGCGCTGGGC GTTTCCGTGG CCCCCAGTTT CTTTCAATCC 
TGCGTCAGCG ATGGCAAAAC CGCCCGCATG GAGGATGTGC TCAAGTTCAA GGTCTCTACG 
AAGATTATGC AGAATATAAT TGATAAGTTT GCCACGCACG ACATCTTCGG GCGCGACAAC 
TATGAGTACT ACGCCCGCGT CACAGGACGC ATTCTCAAGG TGCAGGACGA GTGGATATGC 
AGCTTTCAGT ATCCACCACC GCCACGTGGC AAGTTGGCTC AACAAAAATA CGCATTGCAA 
CATTCTTTGG AGGCGGAGAA GACATGGCTG CAGTGCCAAT GCGAGCGTTG GGGCTTACTG 
GCCCAGGAGG AGTCCCGCTC GACGCCCGCC CTGCTGACCA GCTCCAGTTC CGCATTGGAG 
AACAGTGTCC TGTCGCTGGG CGTTTCCCCC GAACACTCGT TCATCGAGAG CTGCGCCCGT 
CTCTCAGTTT CGCTGGAGGG ACCTGGCATC TATGCCATGA CTTCGTCGCC GTTGCCGGCG 
AAGCGGAATG GCAATGGCAA CGGAAATGCC ACGGACTACG ACTATGATGA CTATGCCGAC 
GATGATGAGG ACAACGATGA GCTGGACGGC GTTCACGACT TTGGTGCCGA ATTGGAGATC 
AGTAGCATAG CTCCGCCGCA GGGAAGTCGT GGCATCTATC GCCACAAGCA GCGAGCCCAG 
CAGCAGCCAC ACCACTACCA ACAACAGCAG CAGCAGCAGC AGGTGCCGCC TCAGCACCAG 
CGGGACTACG ACGAGGACGA CGACGATGAT GAGGATGAGA TGACGTTTAT TAAGCGCCGG 
TATCGACAGA ACAAAAAGAA GCCGGCGCCG CCGAGCAGCA GCCATCATCA GAGGCGATTG 
CGCACTGGCA CCAAGAGTCT CGATGGCGGA GGGGAGAGGC GCACGGGATC GGGAGCAGCG 
GGCGGATCAG GAGGAGGTGC TTCAGGCTCG GCAGTTGGCA ATCCGACGCC TCCCAAGCTC 
AAGCAGCGAA CGTGCAGCCG CTGCAATCGA GGCATTCGGC ACAGTAGCTC GTGCAGCTCC 
AATTCCGCCG GAGCAACTGG CTCGAATAGC GGAGGAGGCG GCAACGGAGG CTCCGGAGCC 
GGAAACGGAG GAGGGGCCGA GGGCGGCATG ACCATGGAGG AGCTGAGGGC CGTCAACCGA 
TATGCGGAGA GCACCAAGAG CCTCTCGTAT CTGCCACAGG AAATGAAAAA GAATCAAGAA 
CCAAGAATAT ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP71E ( NM_001202183.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189112.1 Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa20041
Clone ID Related Accession (Same CDS sequence)	NM_001202183.2
Accession Version	NM_001202183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 71E, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001202183.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGCT GGGCAGAACG CGTCCATAGG CGCGCCGCGG ATTTGGGCAA CGTGGTGACC 
TCCTGCGGCC ACCCAGCCAC CGCTCCCGCC TACCCGTACC GCCTCGAAAA GGTCAAATTC 
GGTGTGCCGC TGGAGGAGGT GTGCAAGCAC AACAACAACA TTCCCGGGCC CCTGCTCGTC 
CTGATCCTCA AACTGAACAA GGAGTCACCG AACCGCCGCG ATGTATTCCG CGCACCCGGC 
CACCAGGGCG CAATGAAGAA GCTCATCCAC TTCCTGCAGG CCGGTCGGCT CGTCAACGTG 
GACAACTATT CGGTGTTCAC CATCGCCAGC GTGCTCAAGA AGTTCCTGCG CAAGATCCCC 
AATGGGATAT TCGGGCGCAG CGGCGAGATG GAGCTGTTCG CCATCAATGA TCTGCAAAAC 
GAAGCCGAGC AAACGGAGCG ACTGCACAGA CTCTTCGGTT CGCTGCCGAA ATACACACAG 
CATCTATTAG TTCTACTGTT CGGCACATTC CGGGTTATCG CGAGCAATGC GGCCCACGCC 
TCCACCGGAA TGACCAGCGA GGCGCTGGGC GTTTCCGTGG CCCCCAGTTT CTTTCAATCC 
TGCGTCAGCG ATGGCAAAAC CGCCCGCATG GAGGATGTGC TCAAGTTCAA GGTCTCTACG 
AAGATTATGC AGAATATAAT TGATAAGTTT GCCACGCACG ACATCTTCGG GCGCGACAAC 
TATGAGTACT ACGCCCGCGT CACAGGACGC ATTCTCAAGG TGCAGGACGA GTGGATATGC 
AGCTTTCAGT ATCCACCACC GCCACGTGGC AAGTTGGCTC AACAAAAATA CGCATTGCAA 
CATTCTTTGG AGGCGGAGAA GACATGGCTG CAGTGCCAAT GCGAGCGTTG GGGCTTACTG 
GCCCAGGAGG AGTCCCGCTC GACGCCCGCC CTGCTGACCA GCTCCAGTTC CGCATTGGAG 
AACAGTGTCC TGTCGCTGGG CGTTTCCCCC GAACACTCGT TCATCGAGAG CTGCGCCCGT 
CTCTCAGTTT CGCTGGAGGG ACCTGGCATC TATGCCATGA CTTCGTCGCC GTTGCCGGCG 
AAGCGGAATG GCAATGGCAA CGGAAATGCC ACGGACTACG ACTATGATGA CTATGCCGAC 
GATGATGAGG ACAACGATGA GCTGGACGGC GTTCACGACT TTGGTGCCGA ATTGGAGATC 
AGTAGCATAG CTCCGCCGCA GGGAAGTCGT GGCATCTATC GCCACAAGCA GCGAGCCCAG 
CAGCAGCCAC ACCACTACCA ACAACAGCAG CAGCAGCAGC AGGTGCCGCC TCAGCACCAG 
CGGGACTACG ACGAGGACGA CGACGATGAT GAGGATGAGA TGACGTTTAT TAAGCGCCGG 
TATCGACAGA ACAAAAAGAA GCCGGCGCCG CCGAGCAGCA GCCATCATCA GAGGCGATTG 
CGCACTGGCA CCAAGAGTCT CGATGGCGGA GGGGAGAGGC GCACGGGATC GGGAGCAGCG 
GGCGGATCAG GAGGAGGTGC TTCAGGCTCG GCAGTTGGCA ATCCGACGCC TCCCAAGCTC 
AAGCAGCGAA CGTGCAGCCG CTGCAATCGA GGCATTCGGC ACAGTAGCTC GTGCAGCTCC 
AATTCCGCCG GAGCAACTGG CTCGAATAGC GGAGGAGGCG GCAACGGAGG CTCCGGAGCC 
GGAAACGGAG GAGGGGCCGA GGGCGGCATG ACCATGGAGG AGCTGAGGGC CGTCAACCGA 
TATGCGGAGA GCACCAAGAG CCTCTCGTAT CTGCCACAGT TGGCAACATC CGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189112.1
CDS	689..2485
Translation	MSSWAERVHRRAADLGNVVTSCGHPATAPAYPYRLEKVKFGVPLEEVCKHNNNIPGPLLVLILKLNKESPNRRDVFRAPGHQGAMKKLIHFLQAGRLVNVDNYSVFTIASVLKKFLRKIPNGIFGRSGEMELFAINDLQNEAEQTERLHRLFGSLPKYTQHLLVLLFGTFRVIASNAAHASTGMTSEALGVSVAPSFFQSCVSDGKTARMEDVLKFKVSTKIMQNIIDKFATHDIFGRDNYEYYARVTGRILKVQDEWICSFQYPPPPRGKLAQQKYALQHSLEAEKTWLQCQCERWGLLAQEESRSTPALLTSSSSALENSVLSLGVSPEHSFIESCARLSVSLEGPGIYAMTSSPLPAKRNGNGNGNATDYDYDDYADDDEDNDELDGVHDFGAELEISSIAPPQGSRGIYRHKQRAQQQPHHYQQQQQQQQVPPQHQRDYDEDDDDDEDEMTFIKRRYRQNKKKPAPPSSSHHQRRLRTGTKSLDGGGERRTGSGAAGGSGGGASGSAVGNPTPPKLKQRTCSRCNRGIRHSSSCSSNSAGATGSNSGGGGNGGSGAGNGGGAEGGMTMEELRAVNRYAESTKSLSYLPQLATSV

Target ORF information:

RefSeq Version	NM_001202183.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202183.2

ORF Insert Sequence:

ATGTCCAGCT GGGCAGAACG CGTCCATAGG CGCGCCGCGG ATTTGGGCAA CGTGGTGACC 
TCCTGCGGCC ACCCAGCCAC CGCTCCCGCC TACCCGTACC GCCTCGAAAA GGTCAAATTC 
GGTGTGCCGC TGGAGGAGGT GTGCAAGCAC AACAACAACA TTCCCGGGCC CCTGCTCGTC 
CTGATCCTCA AACTGAACAA GGAGTCACCG AACCGCCGCG ATGTATTCCG CGCACCCGGC 
CACCAGGGCG CAATGAAGAA GCTCATCCAC TTCCTGCAGG CCGGTCGGCT CGTCAACGTG 
GACAACTATT CGGTGTTCAC CATCGCCAGC GTGCTCAAGA AGTTCCTGCG CAAGATCCCC 
AATGGGATAT TCGGGCGCAG CGGCGAGATG GAGCTGTTCG CCATCAATGA TCTGCAAAAC 
GAAGCCGAGC AAACGGAGCG ACTGCACAGA CTCTTCGGTT CGCTGCCGAA ATACACACAG 
CATCTATTAG TTCTACTGTT CGGCACATTC CGGGTTATCG CGAGCAATGC GGCCCACGCC 
TCCACCGGAA TGACCAGCGA GGCGCTGGGC GTTTCCGTGG CCCCCAGTTT CTTTCAATCC 
TGCGTCAGCG ATGGCAAAAC CGCCCGCATG GAGGATGTGC TCAAGTTCAA GGTCTCTACG 
AAGATTATGC AGAATATAAT TGATAAGTTT GCCACGCACG ACATCTTCGG GCGCGACAAC 
TATGAGTACT ACGCCCGCGT CACAGGACGC ATTCTCAAGG TGCAGGACGA GTGGATATGC 
AGCTTTCAGT ATCCACCACC GCCACGTGGC AAGTTGGCTC AACAAAAATA CGCATTGCAA 
CATTCTTTGG AGGCGGAGAA GACATGGCTG CAGTGCCAAT GCGAGCGTTG GGGCTTACTG 
GCCCAGGAGG AGTCCCGCTC GACGCCCGCC CTGCTGACCA GCTCCAGTTC CGCATTGGAG 
AACAGTGTCC TGTCGCTGGG CGTTTCCCCC GAACACTCGT TCATCGAGAG CTGCGCCCGT 
CTCTCAGTTT CGCTGGAGGG ACCTGGCATC TATGCCATGA CTTCGTCGCC GTTGCCGGCG 
AAGCGGAATG GCAATGGCAA CGGAAATGCC ACGGACTACG ACTATGATGA CTATGCCGAC 
GATGATGAGG ACAACGATGA GCTGGACGGC GTTCACGACT TTGGTGCCGA ATTGGAGATC 
AGTAGCATAG CTCCGCCGCA GGGAAGTCGT GGCATCTATC GCCACAAGCA GCGAGCCCAG 
CAGCAGCCAC ACCACTACCA ACAACAGCAG CAGCAGCAGC AGGTGCCGCC TCAGCACCAG 
CGGGACTACG ACGAGGACGA CGACGATGAT GAGGATGAGA TGACGTTTAT TAAGCGCCGG 
TATCGACAGA ACAAAAAGAA GCCGGCGCCG CCGAGCAGCA GCCATCATCA GAGGCGATTG 
CGCACTGGCA CCAAGAGTCT CGATGGCGGA GGGGAGAGGC GCACGGGATC GGGAGCAGCG 
GGCGGATCAG GAGGAGGTGC TTCAGGCTCG GCAGTTGGCA ATCCGACGCC TCCCAAGCTC 
AAGCAGCGAA CGTGCAGCCG CTGCAATCGA GGCATTCGGC ACAGTAGCTC GTGCAGCTCC 
AATTCCGCCG GAGCAACTGG CTCGAATAGC GGAGGAGGCG GCAACGGAGG CTCCGGAGCC 
GGAAACGGAG GAGGGGCCGA GGGCGGCATG ACCATGGAGG AGCTGAGGGC CGTCAACCGA 
TATGCGGAGA GCACCAAGAG CCTCTCGTAT CTGCCACAGT TGGCAACATC CGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP71E ( NM_001274957.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261886.1 Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant F, mRNA.

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CloneID	OFa20042
Clone ID Related Accession (Same CDS sequence)	NM_001274957.1
Accession Version	NM_001274957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 71E, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGCT GGGCAGAACG CGTCCATAGG CGCGCCGCGG ATTTGGGCAA CGTGGTGACC 
TCCTGCGGCC ACCCAGCCAC CGCTCCCGCC TACCCGTACC GCCTCGAAAA GGTCAAATTC 
GGTGTGCCGC TGGAGGAGGT GTGCAAGCAC AACAACAACA TTCCCGGGCC CCTGCTCGTC 
CTGATCCTCA AACTGAACAA GGAGTCACCG AACCGCCGCG ATGTATTCCG CGCACCCGGC 
CACCAGGGCG CAATGAAGAA GCTCATCCAC TTCCTGCAGG CCGGTCGGCT CGTCAACGTG 
GACAACTATT CGGTGTTCAC CATCGCCAGC GTGCTCAAGA AGTTCCTGCG CAAGATCCCC 
AATGGGATAT TCGGGCGCAG CGGCGAGATG GAGCTGTTCG CCATCAATGA TCTGCAAAAC 
GAAGCCGAGC AAACGGAGCG ACTGCACAGA CTCTTCGGTT CGCTGCCGAA ATACACACAG 
CATCTATTAG TTCTACTGTT CGGCACATTC CGGGTTATCG CGAGCAATGC GGCCCACGCC 
TCCACCGGAA TGACCAGCGA GGCGCTGGGC GTTTCCGTGG CCCCCAGTTT CTTTCAATCC 
TGCGTCAGCG ATGGCAAAAC CGCCCGCATG GAGGATGTGC TCAAGTTCAA GGTCTCTACG 
AAGATTATGC AGAATATAAT TGATAAGTTT GCCACGCACG ACATCTTCGG GCGCGACAAC 
TATGAGTACT ACGCCCGCGT CACAGGACGC ATTCTCAAGG TGCAGGACGA GTGGATATGC 
AGCTTTCAGT ATCCACCACC GCCACGTGGC AAGTTGGCTC AACAAAAATA CGCATTGCAA 
CATTCTTTGG AGGCGGAGAA GACATGGCTG CAGTGCCAAT GCGAGCGTTG GGGCTTACGT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261886.1
CDS	689..1594
Translation	MSSWAERVHRRAADLGNVVTSCGHPATAPAYPYRLEKVKFGVPLEEVCKHNNNIPGPLLVLILKLNKESPNRRDVFRAPGHQGAMKKLIHFLQAGRLVNVDNYSVFTIASVLKKFLRKIPNGIFGRSGEMELFAINDLQNEAEQTERLHRLFGSLPKYTQHLLVLLFGTFRVIASNAAHASTGMTSEALGVSVAPSFFQSCVSDGKTARMEDVLKFKVSTKIMQNIIDKFATHDIFGRDNYEYYARVTGRILKVQDEWICSFQYPPPPRGKLAQQKYALQHSLEAEKTWLQCQCERWGLRP

Target ORF information:

RefSeq Version	NM_001274957.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274957.1

ORF Insert Sequence:

ATGTCCAGCT GGGCAGAACG CGTCCATAGG CGCGCCGCGG ATTTGGGCAA CGTGGTGACC 
TCCTGCGGCC ACCCAGCCAC CGCTCCCGCC TACCCGTACC GCCTCGAAAA GGTCAAATTC 
GGTGTGCCGC TGGAGGAGGT GTGCAAGCAC AACAACAACA TTCCCGGGCC CCTGCTCGTC 
CTGATCCTCA AACTGAACAA GGAGTCACCG AACCGCCGCG ATGTATTCCG CGCACCCGGC 
CACCAGGGCG CAATGAAGAA GCTCATCCAC TTCCTGCAGG CCGGTCGGCT CGTCAACGTG 
GACAACTATT CGGTGTTCAC CATCGCCAGC GTGCTCAAGA AGTTCCTGCG CAAGATCCCC 
AATGGGATAT TCGGGCGCAG CGGCGAGATG GAGCTGTTCG CCATCAATGA TCTGCAAAAC 
GAAGCCGAGC AAACGGAGCG ACTGCACAGA CTCTTCGGTT CGCTGCCGAA ATACACACAG 
CATCTATTAG TTCTACTGTT CGGCACATTC CGGGTTATCG CGAGCAATGC GGCCCACGCC 
TCCACCGGAA TGACCAGCGA GGCGCTGGGC GTTTCCGTGG CCCCCAGTTT CTTTCAATCC 
TGCGTCAGCG ATGGCAAAAC CGCCCGCATG GAGGATGTGC TCAAGTTCAA GGTCTCTACG 
AAGATTATGC AGAATATAAT TGATAAGTTT GCCACGCACG ACATCTTCGG GCGCGACAAC 
TATGAGTACT ACGCCCGCGT CACAGGACGC ATTCTCAAGG TGCAGGACGA GTGGATATGC 
AGCTTTCAGT ATCCACCACC GCCACGTGGC AAGTTGGCTC AACAAAAATA CGCATTGCAA 
CATTCTTTGG AGGCGGAGAA GACATGGCTG CAGTGCCAAT GCGAGCGTTG GGGCTTACGT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP71E ( NM_001274958.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261887.1 Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa20043
Clone ID Related Accession (Same CDS sequence)	NM_001274958.1
Accession Version	NM_001274958.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 71E, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGCT GGGCAGAACG CGTCCATAGG CGCGCCGCGG ATTTGGGCAA CGTGGTGACC 
TCCTGCGGCC ACCCAGCCAC CGCTCCCGCC TACCCGTACC GCCTCGAAAA GGTCAAATTC 
GGTGTGCCGC TGGAGGAGGT GTGCAAGCAC AACAACAACA TTCCCGGGCC CCTGCTCGTC 
CTGATCCTCA AACTGAACAA GGAGTCACCG AACCGCCGCG ATGTATTCCG CGCACCCGGC 
CACCAGGGCG CAATGAAGAA GCTCATCCAC TTCCTGCAGG CCGGTCGGCT CGTCAACGTG 
GACAACTATT CGGTGTTCAC CATCGCCAGC GTGCTCAAGA AGTTCCTGCG CAAGATCCCC 
AATGGGATAT TCGGGCGCAG CGGCGAGATG GAGCTGTTCG CCATCAATGA TCTGCAAAAC 
GAAGCCGAGC AAACGGAGCG ACTGCACAGA CTCTTCGGTT CGCTGCCGAA ATACACACAG 
CATCTATTAG TTCTACTGTT CGGCACATTC CGGGTTATCG CGAGCAATGC GGCCCACGCC 
TCCACCGGAA TGACCAGCGA GGCGCTGGGC GTTTCCGTGG CCCCCAGTTT CTTTCAATCC 
TGCGTCAGCG ATGGCAAAAC CGCCCGCATG GAGGATGTGC TCAAGTTCAA GGTCTCTACG 
AAGATTATGC AGAATATAAT TGATAAGTTT GCCACGCACG ACATCTTCGG GCGCGACAAC 
TATGAGTACT ACGCCCGCGT CACAGGACGC ATTCTCAAGG TGCAGGACGA GTGGATATGC 
AGCTTTCAGT ATCCACCACC GCCACGTGGC AAGTTGGCTC AACAAAAATA CGCATTGCAA 
CATTCTTTGG AGGCGGAGAA GACATGGCTG CAGTGCCAAT GCGAGCGTTG GGGCTTACTT 
GGCAACATCC GTTTGAGTAA CAGCGCCGAG TCCATTCAGT ATGCAGCCCG ACGACCGGTC 
GCCAGTCTTG GTCTCAATCC CAGCCTCGGC GGCGCCATTA CCACCATCAA CACCACCAGC 
ACCCACAATC CCACCACCAG GCGTCTGCTC ATCAATGTGC CGCGCCAGTA TCGATCCTCG 
CTGCGGCGCA ACAAGGAGAA GCAGATCGGC GGCAATAGTC AGAGCAACAG TAGCAATAGC 
CTGGGCAGTG ATCAGTTGAC CGGAAATGCT GGAGCAGCTG CAGGATCGGC CGTCGGTGTT 
CCGGTGCTAA ACAAACCCCT ACTGATGGTC GGCCTACTGC CGGGTGCCGA GCAGCCGCCC 
TCCGTGCGGA ACTCCATTGC CGAGTGCGGC ATCTCGACGC CGGATGCAAT GGACGTGGGC 
ATGGAGTGCA GCACCACGCT GAGCTTCAAG CCGCCGCGGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261887.1
CDS	689..2053
Translation	MSSWAERVHRRAADLGNVVTSCGHPATAPAYPYRLEKVKFGVPLEEVCKHNNNIPGPLLVLILKLNKESPNRRDVFRAPGHQGAMKKLIHFLQAGRLVNVDNYSVFTIASVLKKFLRKIPNGIFGRSGEMELFAINDLQNEAEQTERLHRLFGSLPKYTQHLLVLLFGTFRVIASNAAHASTGMTSEALGVSVAPSFFQSCVSDGKTARMEDVLKFKVSTKIMQNIIDKFATHDIFGRDNYEYYARVTGRILKVQDEWICSFQYPPPPRGKLAQQKYALQHSLEAEKTWLQCQCERWGLLGNIRLSNSAESIQYAARRPVASLGLNPSLGGAITTINTTSTHNPTTRRLLINVPRQYRSSLRRNKEKQIGGNSQSNSSNSLGSDQLTGNAGAAAGSAVGVPVLNKPLLMVGLLPGAEQPPSVRNSIAECGISTPDAMDVGMECSTTLSFKPPRL

Target ORF information:

RefSeq Version	NM_001274958.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274958.1

ORF Insert Sequence:

ATGTCCAGCT GGGCAGAACG CGTCCATAGG CGCGCCGCGG ATTTGGGCAA CGTGGTGACC 
TCCTGCGGCC ACCCAGCCAC CGCTCCCGCC TACCCGTACC GCCTCGAAAA GGTCAAATTC 
GGTGTGCCGC TGGAGGAGGT GTGCAAGCAC AACAACAACA TTCCCGGGCC CCTGCTCGTC 
CTGATCCTCA AACTGAACAA GGAGTCACCG AACCGCCGCG ATGTATTCCG CGCACCCGGC 
CACCAGGGCG CAATGAAGAA GCTCATCCAC TTCCTGCAGG CCGGTCGGCT CGTCAACGTG 
GACAACTATT CGGTGTTCAC CATCGCCAGC GTGCTCAAGA AGTTCCTGCG CAAGATCCCC 
AATGGGATAT TCGGGCGCAG CGGCGAGATG GAGCTGTTCG CCATCAATGA TCTGCAAAAC 
GAAGCCGAGC AAACGGAGCG ACTGCACAGA CTCTTCGGTT CGCTGCCGAA ATACACACAG 
CATCTATTAG TTCTACTGTT CGGCACATTC CGGGTTATCG CGAGCAATGC GGCCCACGCC 
TCCACCGGAA TGACCAGCGA GGCGCTGGGC GTTTCCGTGG CCCCCAGTTT CTTTCAATCC 
TGCGTCAGCG ATGGCAAAAC CGCCCGCATG GAGGATGTGC TCAAGTTCAA GGTCTCTACG 
AAGATTATGC AGAATATAAT TGATAAGTTT GCCACGCACG ACATCTTCGG GCGCGACAAC 
TATGAGTACT ACGCCCGCGT CACAGGACGC ATTCTCAAGG TGCAGGACGA GTGGATATGC 
AGCTTTCAGT ATCCACCACC GCCACGTGGC AAGTTGGCTC AACAAAAATA CGCATTGCAA 
CATTCTTTGG AGGCGGAGAA GACATGGCTG CAGTGCCAAT GCGAGCGTTG GGGCTTACTT 
GGCAACATCC GTTTGAGTAA CAGCGCCGAG TCCATTCAGT ATGCAGCCCG ACGACCGGTC 
GCCAGTCTTG GTCTCAATCC CAGCCTCGGC GGCGCCATTA CCACCATCAA CACCACCAGC 
ACCCACAATC CCACCACCAG GCGTCTGCTC ATCAATGTGC CGCGCCAGTA TCGATCCTCG 
CTGCGGCGCA ACAAGGAGAA GCAGATCGGC GGCAATAGTC AGAGCAACAG TAGCAATAGC 
CTGGGCAGTG ATCAGTTGAC CGGAAATGCT GGAGCAGCTG CAGGATCGGC CGTCGGTGTT 
CCGGTGCTAA ACAAACCCCT ACTGATGGTC GGCCTACTGC CGGGTGCCGA GCAGCCGCCC 
TCCGTGCGGA ACTCCATTGC CGAGTGCGGC ATCTCGACGC CGGATGCAAT GGACGTGGGC 
ATGGAGTGCA GCACCACGCT GAGCTTCAAG CCGCCGCGGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP71E ( NM_001144484.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137956.1 Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa18565
Clone ID Related Accession (Same CDS sequence)	NM_001144484.3
Accession Version	NM_001144484.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 71E, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001144484.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGCT GGGCAGAACG CGTCCATAGG CGCGCCGCGG ATTTGGGCAA CGTGGTGACC 
TCCTGCGGCC ACCCAGCCAC CGCTCCCGCC TACCCGTACC GCCTCGAAAA GGTCAAATTC 
GGTGTGCCGC TGGAGGAGGT GTGCAAGCAC AACAACAACA TTCCCGGGCC CCTGCTCGTC 
CTGATCCTCA AACTGAACAA GGAGTCACCG AACCGCCGCG ATGTATTCCG CGCACCCGGC 
CACCAGGGCG CAATGAAGAA GCTCATCCAC TTCCTGCAGG CCGGTCGGCT CGTCAACGTG 
GACAACTATT CGGTGTTCAC CATCGCCAGC GTGCTCAAGA AGTTCCTGCG CAAGATCCCC 
AATGGGATAT TCGGGCGCAG CGGCGAGATG GAGCTGTTCG CCATCAATGA TCTGCAAAAC 
GAAGCCGAGC AAACGGAGCG ACTGCACAGA CTCTTCGGTT CGCTGCCGAA ATACACACAG 
CATCTATTAG TTCTACTGTT CGGCACATTC CGGGTTATCG CGAGCAATGC GGCCCACGCC 
TCCACCGGAA TGACCAGCGA GGCGCTGGGC GTTTCCGTGG CCCCCAGTTT CTTTCAATCC 
TGCGTCAGCG ATGGCAAAAC CGCCCGCATG GAGGATGTGC TCAAGTTCAA GGTCTCTACG 
AAGATTATGC AGAATATAAT TGATAAGTTT GCCACGCACG ACATCTTCGG GCGCGACAAC 
TATGAGTACT ACGCCCGCGT CACAGGACGC ATTCTCAAGG TGCAGGACGA GTGGATATGC 
AGCTTTCAGT ATCCACCACC GCCACGTGGC AAGTTGGCTC AACAAAAATA CGCATTGCAA 
CATTCTTTGG AGGCGGAGAA GACATGGCTG CAGTGCCAAT GCGAGCGTTG GGGCTTACTG 
GCCCAGGAGG AGTCCCGCTC GACGCCCGCC CTGCTGACCA GCTCCAGTTC CGCATTGGAG 
AACAGTGTCC TGTCGCTGGG CGTTTCCCCC GAACACTCGT TCATCGAGAG CTGCGCCCGT 
CTCTCAGTTT CGCTGGAGGG ACCTGGCATC TATGCCATGA CTTCGTCGCC GTTGCCGGCG 
AAGCGGAATG GCAATGGCAA CGGAAATGCC ACGGACTACG ACTATGATGA CTATGCCGAC 
GATGATGAGG ACAACGATGA GCTGGACGGC GTTCACGACT TTGGTGCCGA ATTGGAGATC 
AGTAGCATAG CTCCGCCGCA GGGAAGTCGT GGCATCTATC GCCACAAGCA GCGAGCCCAG 
CAGCAGCCAC ACCACTACCA ACAACAGCAG CAGCAGCAGC AGGTGCCGCC TCAGCACCAG 
CGGGACTACG ACGAGGACGA CGACGATGAT GAGGATGAGA TGACGTTTAT TAAGCGCCGG 
TATCGACAGA ACAAAAAGAA GCCGGCGCCG CCGAGCAGCA GCCATCATCA GAGGCGATTG 
CGCACTGGCA CCAAGAGTCT CGATGGCGGA GGGGAGAGGC GCACGGGATC GGGAGCAGCG 
GGCGGATCAG GAGGAGGTGC TTCAGGCTCG GCAGTTGGCA ATCCGACGCC TCCCAAGCTC 
AAGCAGCGAA CGTGCAGCCG CTGCAATCGA GGCATTCGGC ACAGTAGCTC GTGCAGCTCC 
AATTCCGCCG GAGCAACTGG CTCGAATAGC GGAGGAGGCG GCAACGGAGG CTCCGGAGCC 
GGAAACGGAG GAGGGGCCGA GGGCGGCATG ACCATGGAGG AGCTGAGGGC CGTCAACCGA 
TATGCGGAGA GCACCAAGAG CCTCTCGTAT CTGCCACAGG TCCATGAACG CCAAACCGCA 
CGCATGCGCA CCCGCTCCGA GTGGTTCCTG GGTCCCCGGG ATGCGGTGGT CACGTTGCAA 
TTGCCGGCGG ACACGTGCAG CAATTGCTGT CTTGCCGCAG CCGCTGGATA TTTGGGTGGC 
GGCAGCACCA GGGACATGCG ACAGGCTCAA ACGGAAGGCG AGCGGAATCA GGCGGCCTTG 
CTCTATCACT TTTACCGCAG GCAGCAGCAG CAGCAACAGC AACAGCAGGA GCAACACTAT 
GACGATATGA TGGACACGGA GGATAGCTAC GGACAGCAGA AGGAGCAACA GCATCAGCAG 
CAGCAGCAGC AGGAGCAGCA ACAGCAACAC ATACATTCCA AGGGTCTACT AAGACCACCG 
CCGATGCAAC ATCAGCATCA ACAGCAGCAG CAGCAACGTC GCCTGCTGCT GGATGTCAAT 
TCGGGTAGTT ATAACGCTGG ACTAACTACG CTCACCACCA ACAACACTCA TTTCGGCGAC 
GATCTGGCCA ACTATCTACA CGAACCGCTC AGCAACACAT GCTATTATTC CTCCAGCCAG 
TGCTTGAACC GGCTGCCACC TGTTGTCGTT GTGCGCGAGG ATCAGCAGCT GACGGAGGTC 
ACCGATAGCG ATGGCGATGG CGACTCTCTG GACGGCGGTG TTTTGCACAC CCTGCAGGCC 
CACGAGTTGC AGCATCATGT GGAGCCCAGG CAGGCGGCCA TGTTGCTGCT GCGACACTCG 
ACACCGCAGC AGCAACATCG CAGCAGCATG GCCTCGTCCT TGTACCAATC CTTTGACGTG 
ACCTCCGCCG ATCTGAGCCA CGGCAACGAC CACAACAGGA GCGGCCACAA TAATAGCAAC 
AATAACCATC ACCACAACAA CAACTTTGCC ACAGTCATTT TCAATGTTTT GGACTTTTTT 
TGGCAACATC CGTTTGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137956.1
CDS	689..3409
Translation	MSSWAERVHRRAADLGNVVTSCGHPATAPAYPYRLEKVKFGVPLEEVCKHNNNIPGPLLVLILKLNKESPNRRDVFRAPGHQGAMKKLIHFLQAGRLVNVDNYSVFTIASVLKKFLRKIPNGIFGRSGEMELFAINDLQNEAEQTERLHRLFGSLPKYTQHLLVLLFGTFRVIASNAAHASTGMTSEALGVSVAPSFFQSCVSDGKTARMEDVLKFKVSTKIMQNIIDKFATHDIFGRDNYEYYARVTGRILKVQDEWICSFQYPPPPRGKLAQQKYALQHSLEAEKTWLQCQCERWGLLAQEESRSTPALLTSSSSALENSVLSLGVSPEHSFIESCARLSVSLEGPGIYAMTSSPLPAKRNGNGNGNATDYDYDDYADDDEDNDELDGVHDFGAELEISSIAPPQGSRGIYRHKQRAQQQPHHYQQQQQQQQVPPQHQRDYDEDDDDDEDEMTFIKRRYRQNKKKPAPPSSSHHQRRLRTGTKSLDGGGERRTGSGAAGGSGGGASGSAVGNPTPPKLKQRTCSRCNRGIRHSSSCSSNSAGATGSNSGGGGNGGSGAGNGGGAEGGMTMEELRAVNRYAESTKSLSYLPQVHERQTARMRTRSEWFLGPRDAVVTLQLPADTCSNCCLAAAAGYLGGGSTRDMRQAQTEGERNQAALLYHFYRRQQQQQQQQQEQHYDDMMDTEDSYGQQKEQQHQQQQQQEQQQQHIHSKGLLRPPPMQHQHQQQQQQRRLLLDVNSGSYNAGLTTLTTNNTHFGDDLANYLHEPLSNTCYYSSSQCLNRLPPVVVVREDQQLTEVTDSDGDGDSLDGGVLHTLQAHELQHHVEPRQAAMLLLRHSTPQQQHRSSMASSLYQSFDVTSADLSHGNDHNRSGHNNSNNNHHHNNNFATVIFNVLDFFWQHPFE

Target ORF information:

RefSeq Version	NM_001144484.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144484.3

ORF Insert Sequence:

ATGTCCAGCT GGGCAGAACG CGTCCATAGG CGCGCCGCGG ATTTGGGCAA CGTGGTGACC 
TCCTGCGGCC ACCCAGCCAC CGCTCCCGCC TACCCGTACC GCCTCGAAAA GGTCAAATTC 
GGTGTGCCGC TGGAGGAGGT GTGCAAGCAC AACAACAACA TTCCCGGGCC CCTGCTCGTC 
CTGATCCTCA AACTGAACAA GGAGTCACCG AACCGCCGCG ATGTATTCCG CGCACCCGGC 
CACCAGGGCG CAATGAAGAA GCTCATCCAC TTCCTGCAGG CCGGTCGGCT CGTCAACGTG 
GACAACTATT CGGTGTTCAC CATCGCCAGC GTGCTCAAGA AGTTCCTGCG CAAGATCCCC 
AATGGGATAT TCGGGCGCAG CGGCGAGATG GAGCTGTTCG CCATCAATGA TCTGCAAAAC 
GAAGCCGAGC AAACGGAGCG ACTGCACAGA CTCTTCGGTT CGCTGCCGAA ATACACACAG 
CATCTATTAG TTCTACTGTT CGGCACATTC CGGGTTATCG CGAGCAATGC GGCCCACGCC 
TCCACCGGAA TGACCAGCGA GGCGCTGGGC GTTTCCGTGG CCCCCAGTTT CTTTCAATCC 
TGCGTCAGCG ATGGCAAAAC CGCCCGCATG GAGGATGTGC TCAAGTTCAA GGTCTCTACG 
AAGATTATGC AGAATATAAT TGATAAGTTT GCCACGCACG ACATCTTCGG GCGCGACAAC 
TATGAGTACT ACGCCCGCGT CACAGGACGC ATTCTCAAGG TGCAGGACGA GTGGATATGC 
AGCTTTCAGT ATCCACCACC GCCACGTGGC AAGTTGGCTC AACAAAAATA CGCATTGCAA 
CATTCTTTGG AGGCGGAGAA GACATGGCTG CAGTGCCAAT GCGAGCGTTG GGGCTTACTG 
GCCCAGGAGG AGTCCCGCTC GACGCCCGCC CTGCTGACCA GCTCCAGTTC CGCATTGGAG 
AACAGTGTCC TGTCGCTGGG CGTTTCCCCC GAACACTCGT TCATCGAGAG CTGCGCCCGT 
CTCTCAGTTT CGCTGGAGGG ACCTGGCATC TATGCCATGA CTTCGTCGCC GTTGCCGGCG 
AAGCGGAATG GCAATGGCAA CGGAAATGCC ACGGACTACG ACTATGATGA CTATGCCGAC 
GATGATGAGG ACAACGATGA GCTGGACGGC GTTCACGACT TTGGTGCCGA ATTGGAGATC 
AGTAGCATAG CTCCGCCGCA GGGAAGTCGT GGCATCTATC GCCACAAGCA GCGAGCCCAG 
CAGCAGCCAC ACCACTACCA ACAACAGCAG CAGCAGCAGC AGGTGCCGCC TCAGCACCAG 
CGGGACTACG ACGAGGACGA CGACGATGAT GAGGATGAGA TGACGTTTAT TAAGCGCCGG 
TATCGACAGA ACAAAAAGAA GCCGGCGCCG CCGAGCAGCA GCCATCATCA GAGGCGATTG 
CGCACTGGCA CCAAGAGTCT CGATGGCGGA GGGGAGAGGC GCACGGGATC GGGAGCAGCG 
GGCGGATCAG GAGGAGGTGC TTCAGGCTCG GCAGTTGGCA ATCCGACGCC TCCCAAGCTC 
AAGCAGCGAA CGTGCAGCCG CTGCAATCGA GGCATTCGGC ACAGTAGCTC GTGCAGCTCC 
AATTCCGCCG GAGCAACTGG CTCGAATAGC GGAGGAGGCG GCAACGGAGG CTCCGGAGCC 
GGAAACGGAG GAGGGGCCGA GGGCGGCATG ACCATGGAGG AGCTGAGGGC CGTCAACCGA 
TATGCGGAGA GCACCAAGAG CCTCTCGTAT CTGCCACAGG TCCATGAACG CCAAACCGCA 
CGCATGCGCA CCCGCTCCGA GTGGTTCCTG GGTCCCCGGG ATGCGGTGGT CACGTTGCAA 
TTGCCGGCGG ACACGTGCAG CAATTGCTGT CTTGCCGCAG CCGCTGGATA TTTGGGTGGC 
GGCAGCACCA GGGACATGCG ACAGGCTCAA ACGGAAGGCG AGCGGAATCA GGCGGCCTTG 
CTCTATCACT TTTACCGCAG GCAGCAGCAG CAGCAACAGC AACAGCAGGA GCAACACTAT 
GACGATATGA TGGACACGGA GGATAGCTAC GGACAGCAGA AGGAGCAACA GCATCAGCAG 
CAGCAGCAGC AGGAGCAGCA ACAGCAACAC ATACATTCCA AGGGTCTACT AAGACCACCG 
CCGATGCAAC ATCAGCATCA ACAGCAGCAG CAGCAACGTC GCCTGCTGCT GGATGTCAAT 
TCGGGTAGTT ATAACGCTGG ACTAACTACG CTCACCACCA ACAACACTCA TTTCGGCGAC 
GATCTGGCCA ACTATCTACA CGAACCGCTC AGCAACACAT GCTATTATTC CTCCAGCCAG 
TGCTTGAACC GGCTGCCACC TGTTGTCGTT GTGCGCGAGG ATCAGCAGCT GACGGAGGTC 
ACCGATAGCG ATGGCGATGG CGACTCTCTG GACGGCGGTG TTTTGCACAC CCTGCAGGCC 
CACGAGTTGC AGCATCATGT GGAGCCCAGG CAGGCGGCCA TGTTGCTGCT GCGACACTCG 
ACACCGCAGC AGCAACATCG CAGCAGCATG GCCTCGTCCT TGTACCAATC CTTTGACGTG 
ACCTCCGCCG ATCTGAGCCA CGGCAACGAC CACAACAGGA GCGGCCACAA TAATAGCAAC 
AATAACCATC ACCACAACAA CAACTTTGCC ACAGTCATTT TCAATGTTTT GGACTTTTTT 
TGGCAACATC CGTTTGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP71E ( NM_168629.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730060.1 Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant C, mRNA.

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CloneID	OFa18566
Clone ID Related Accession (Same CDS sequence)	NM_168629.4
Accession Version	NM_168629.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 71E, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168629.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGCT GGGCAGAACG CGTCCATAGG CGCGCCGCGG ATTTGGGCAA CGTGGTGACC 
TCCTGCGGCC ACCCAGCCAC CGCTCCCGCC TACCCGTACC GCCTCGAAAA GGTCAAATTC 
GGTGTGCCGC TGGAGGAGGT GTGCAAGCAC AACAACAACA TTCCCGGGCC CCTGCTCGTC 
CTGATCCTCA AACTGAACAA GGAGTCACCG AACCGCCGCG ATGTATTCCG CGCACCCGGC 
CACCAGGGCG CAATGAAGAA GCTCATCCAC TTCCTGCAGG CCGGTCGGCT CGTCAACGTG 
GACAACTATT CGGTGTTCAC CATCGCCAGC GTGCTCAAGA AGTTCCTGCG CAAGATCCCC 
AATGGGATAT TCGGGCGCAG CGGCGAGATG GAGCTGTTCG CCATCAATGA TCTGCAAAAC 
GAAGCCGAGC AAACGGAGCG ACTGCACAGA CTCTTCGGTT CGCTGCCGAA ATACACACAG 
CATCTATTAG TTCTACTGTT CGGCACATTC CGGGTTATCG CGAGCAATGC GGCCCACGCC 
TCCACCGGAA TGACCAGCGA GGCGCTGGGC GTTTCCGTGG CCCCCAGTTT CTTTCAATCC 
TGCGTCAGCG ATGGCAAAAC CGCCCGCATG GAGGATGTGC TCAAGTTCAA GGTCTCTACG 
AAGATTATGC AGAATATAAT TGATAAGTTT GCCACGCACG ACATCTTCGG GCGCGACAAC 
TATGAGTACT ACGCCCGCGT CACAGGACGC ATTCTCAAGG TGCAGGACGA GTGGATATGC 
AGCTTTCAGT ATCCACCACC GCCACGTGGC AAGTTGGCTC AACAAAAATA CGCATTGCAA 
CATTCTTTGG AGGCGGAGAA GACATGGCTG CAGTGCCAAT GCGAGCGTTG GGGCTTACTG 
GCCCAGGAGG AGTCCCGCTC GACGCCCGCC CTGCTGACCA GCTCCAGTTC CGCATTGGAG 
AACAGTGTCC TGTCGCTGGG CGTTTCCCCC GAACACTCGT TCATCGAGAG CTGCGCCCGT 
CTCTCAGTTT CGCTGGAGGG ACCTGGCATC TATGCCATGA CTTCGTCGCC GTTGCCGGCG 
AAGCGGAATG GCAATGGCAA CGGAAATGCC ACGGACTACG ACTATGATGA CTATGCCGAC 
GATGATGAGG ACAACGATGA GCTGGACGGC GTTCACGACT TTGGTGCCGA ATTGGAGATC 
AGTAGCATAG CTCCGCCGCA GGGAAGTCGT GGCATCTATC GCCACAAGCA GCGAGCCCAG 
CAGCAGCCAC ACCACTACCA ACAACAGCAG CAGCAGCAGC AGGTGCCGCC TCAGCACCAG 
CGGGACTACG ACGAGGACGA CGACGATGAT GAGGATGAGA TGACGTTTAT TAAGCGCCGG 
TATCGACAGA ACAAAAAGAA GCCGGCGCCG CCGAGCAGCA GCCATCATCA GAGGCGATTG 
CGCACTGGCA CCAAGAGTCT CGATGGCGGA GGGGAGAGGC GCACGGGATC GGGAGCAGCG 
GGCGGATCAG GAGGAGGTGC TTCAGGCTCG GCAGTTGGCA ATCCGACGCC TCCCAAGCTC 
AAGCAGCGAA CGTGCAGCCG CTGCAATCGA GGCATTCGGC ACAGTAGCTC GTGCAGCTCC 
AATTCCGCCG GAGCAACTGG CTCGAATAGC GGAGGAGGCG GCAACGGAGG CTCCGGAGCC 
GGAAACGGAG GAGGGGCCGA GGGCGGCATG ACCATGGAGG AGCTGAGGGC CGTCAACCGA 
TATGCGGAGA GCACCAAGAG CCTCTCGTAT CTGCCACAGG TCCATGAACG CCAAACCGCA 
CGCATGCGCA CCCGCTCCGA GTGGTTCCTG GGTCCCCGGG ATGCGGTGGT CACGTTGCAA 
TTGCCGGCGG ACACGTGCAG CAATTGCTGT CTTGCCGCAG CCGCTGGATA TTTGGGTGGC 
GGCAGCACCA GGGACATGCG ACAGGCTCAA ACGGAAGGCG AGCGGAATCA GGCGGCCTTG 
CTCTATCACT TTTACCGCAG GCAGCAGCAG CAGCAACAGC AACAGCAGGA GCAACACTAT 
GACGATATGA TGGACACGGA GGATAGCTAC GGACAGCAGA AGGAGCAACA GCATCAGCAG 
CAGCAGCAGC AGGAGCAGCA ACAGCAACAC ATACATTCCA AGGGTCTACT AAGACCACCG 
CCGATGCAAC ATCAGCATCA ACAGCAGCAG CAGCAACGTC GCCTGCTGCT GGATGTCAAT 
TCGGTTGGCA ACATCCGTTT GAGTAACAGC GCCGAGTCCA TTCAGTATGC AGCCCGACGA 
CCGGTCGCCA GTCTTGGTCT CAATCCCAGC CTCGGCGGCG CCATTACCAC CATCAACACC 
ACCAGCACCC ACAATCCCAC CACCAGGCGT CTGCTCATCA ATGTGCCGCG CCAGTATCGA 
TCCTCGCTGC GGCGCAACAA GGAGAAGCAG ATCGGCGGCA ATAGTCAGAG CAACAGTAGC 
AATAGCCTGG GCAGTGATCA GTTGACCGGA AATGCTGGAG CAGCTGCAGG ATCGGCCGTC 
GGTGTTCCGG TGCTAAACAA ACCCCTACTG ATGGTCGGCC TACTGCCGGG TGCCGAGCAG 
CCGCCCTCCG TGCGGAACTC CATTGCCGAG TGCGGCATCT CGACGCCGGA TGCAATGGAC 
GTGGGCATGG AGTGCAGCAC CACGCTGAGC TTCAAGCCGC CGCGGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730060.1
CDS	689..3379
Translation	MSSWAERVHRRAADLGNVVTSCGHPATAPAYPYRLEKVKFGVPLEEVCKHNNNIPGPLLVLILKLNKESPNRRDVFRAPGHQGAMKKLIHFLQAGRLVNVDNYSVFTIASVLKKFLRKIPNGIFGRSGEMELFAINDLQNEAEQTERLHRLFGSLPKYTQHLLVLLFGTFRVIASNAAHASTGMTSEALGVSVAPSFFQSCVSDGKTARMEDVLKFKVSTKIMQNIIDKFATHDIFGRDNYEYYARVTGRILKVQDEWICSFQYPPPPRGKLAQQKYALQHSLEAEKTWLQCQCERWGLLAQEESRSTPALLTSSSSALENSVLSLGVSPEHSFIESCARLSVSLEGPGIYAMTSSPLPAKRNGNGNGNATDYDYDDYADDDEDNDELDGVHDFGAELEISSIAPPQGSRGIYRHKQRAQQQPHHYQQQQQQQQVPPQHQRDYDEDDDDDEDEMTFIKRRYRQNKKKPAPPSSSHHQRRLRTGTKSLDGGGERRTGSGAAGGSGGGASGSAVGNPTPPKLKQRTCSRCNRGIRHSSSCSSNSAGATGSNSGGGGNGGSGAGNGGGAEGGMTMEELRAVNRYAESTKSLSYLPQVHERQTARMRTRSEWFLGPRDAVVTLQLPADTCSNCCLAAAAGYLGGGSTRDMRQAQTEGERNQAALLYHFYRRQQQQQQQQQEQHYDDMMDTEDSYGQQKEQQHQQQQQQEQQQQHIHSKGLLRPPPMQHQHQQQQQQRRLLLDVNSVGNIRLSNSAESIQYAARRPVASLGLNPSLGGAITTINTTSTHNPTTRRLLINVPRQYRSSLRRNKEKQIGGNSQSNSSNSLGSDQLTGNAGAAAGSAVGVPVLNKPLLMVGLLPGAEQPPSVRNSIAECGISTPDAMDVGMECSTTLSFKPPRL

Target ORF information:

RefSeq Version	NM_168629.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 71E (RhoGAP71E), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168629.4

ORF Insert Sequence:

ATGTCCAGCT GGGCAGAACG CGTCCATAGG CGCGCCGCGG ATTTGGGCAA CGTGGTGACC 
TCCTGCGGCC ACCCAGCCAC CGCTCCCGCC TACCCGTACC GCCTCGAAAA GGTCAAATTC 
GGTGTGCCGC TGGAGGAGGT GTGCAAGCAC AACAACAACA TTCCCGGGCC CCTGCTCGTC 
CTGATCCTCA AACTGAACAA GGAGTCACCG AACCGCCGCG ATGTATTCCG CGCACCCGGC 
CACCAGGGCG CAATGAAGAA GCTCATCCAC TTCCTGCAGG CCGGTCGGCT CGTCAACGTG 
GACAACTATT CGGTGTTCAC CATCGCCAGC GTGCTCAAGA AGTTCCTGCG CAAGATCCCC 
AATGGGATAT TCGGGCGCAG CGGCGAGATG GAGCTGTTCG CCATCAATGA TCTGCAAAAC 
GAAGCCGAGC AAACGGAGCG ACTGCACAGA CTCTTCGGTT CGCTGCCGAA ATACACACAG 
CATCTATTAG TTCTACTGTT CGGCACATTC CGGGTTATCG CGAGCAATGC GGCCCACGCC 
TCCACCGGAA TGACCAGCGA GGCGCTGGGC GTTTCCGTGG CCCCCAGTTT CTTTCAATCC 
TGCGTCAGCG ATGGCAAAAC CGCCCGCATG GAGGATGTGC TCAAGTTCAA GGTCTCTACG 
AAGATTATGC AGAATATAAT TGATAAGTTT GCCACGCACG ACATCTTCGG GCGCGACAAC 
TATGAGTACT ACGCCCGCGT CACAGGACGC ATTCTCAAGG TGCAGGACGA GTGGATATGC 
AGCTTTCAGT ATCCACCACC GCCACGTGGC AAGTTGGCTC AACAAAAATA CGCATTGCAA 
CATTCTTTGG AGGCGGAGAA GACATGGCTG CAGTGCCAAT GCGAGCGTTG GGGCTTACTG 
GCCCAGGAGG AGTCCCGCTC GACGCCCGCC CTGCTGACCA GCTCCAGTTC CGCATTGGAG 
AACAGTGTCC TGTCGCTGGG CGTTTCCCCC GAACACTCGT TCATCGAGAG CTGCGCCCGT 
CTCTCAGTTT CGCTGGAGGG ACCTGGCATC TATGCCATGA CTTCGTCGCC GTTGCCGGCG 
AAGCGGAATG GCAATGGCAA CGGAAATGCC ACGGACTACG ACTATGATGA CTATGCCGAC 
GATGATGAGG ACAACGATGA GCTGGACGGC GTTCACGACT TTGGTGCCGA ATTGGAGATC 
AGTAGCATAG CTCCGCCGCA GGGAAGTCGT GGCATCTATC GCCACAAGCA GCGAGCCCAG 
CAGCAGCCAC ACCACTACCA ACAACAGCAG CAGCAGCAGC AGGTGCCGCC TCAGCACCAG 
CGGGACTACG ACGAGGACGA CGACGATGAT GAGGATGAGA TGACGTTTAT TAAGCGCCGG 
TATCGACAGA ACAAAAAGAA GCCGGCGCCG CCGAGCAGCA GCCATCATCA GAGGCGATTG 
CGCACTGGCA CCAAGAGTCT CGATGGCGGA GGGGAGAGGC GCACGGGATC GGGAGCAGCG 
GGCGGATCAG GAGGAGGTGC TTCAGGCTCG GCAGTTGGCA ATCCGACGCC TCCCAAGCTC 
AAGCAGCGAA CGTGCAGCCG CTGCAATCGA GGCATTCGGC ACAGTAGCTC GTGCAGCTCC 
AATTCCGCCG GAGCAACTGG CTCGAATAGC GGAGGAGGCG GCAACGGAGG CTCCGGAGCC 
GGAAACGGAG GAGGGGCCGA GGGCGGCATG ACCATGGAGG AGCTGAGGGC CGTCAACCGA 
TATGCGGAGA GCACCAAGAG CCTCTCGTAT CTGCCACAGG TCCATGAACG CCAAACCGCA 
CGCATGCGCA CCCGCTCCGA GTGGTTCCTG GGTCCCCGGG ATGCGGTGGT CACGTTGCAA 
TTGCCGGCGG ACACGTGCAG CAATTGCTGT CTTGCCGCAG CCGCTGGATA TTTGGGTGGC 
GGCAGCACCA GGGACATGCG ACAGGCTCAA ACGGAAGGCG AGCGGAATCA GGCGGCCTTG 
CTCTATCACT TTTACCGCAG GCAGCAGCAG CAGCAACAGC AACAGCAGGA GCAACACTAT 
GACGATATGA TGGACACGGA GGATAGCTAC GGACAGCAGA AGGAGCAACA GCATCAGCAG 
CAGCAGCAGC AGGAGCAGCA ACAGCAACAC ATACATTCCA AGGGTCTACT AAGACCACCG 
CCGATGCAAC ATCAGCATCA ACAGCAGCAG CAGCAACGTC GCCTGCTGCT GGATGTCAAT 
TCGGTTGGCA ACATCCGTTT GAGTAACAGC GCCGAGTCCA TTCAGTATGC AGCCCGACGA 
CCGGTCGCCA GTCTTGGTCT CAATCCCAGC CTCGGCGGCG CCATTACCAC CATCAACACC 
ACCAGCACCC ACAATCCCAC CACCAGGCGT CTGCTCATCA ATGTGCCGCG CCAGTATCGA 
TCCTCGCTGC GGCGCAACAA GGAGAAGCAG ATCGGCGGCA ATAGTCAGAG CAACAGTAGC 
AATAGCCTGG GCAGTGATCA GTTGACCGGA AATGCTGGAG CAGCTGCAGG ATCGGCCGTC 
GGTGTTCCGG TGCTAAACAA ACCCCTACTG ATGGTCGGCC TACTGCCGGG TGCCGAGCAG 
CCGCCCTCCG TGCGGAACTC CATTGCCGAG TGCGGCATCT CGACGCCGGA TGCAATGGAC 
GTGGGCATGG AGTGCAGCAC CACGCTGAGC TTCAAGCCGC CGCGGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RhoGAP71E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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