AGO2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGO2
Entrez Gene ID	39683
Full Name	Argonaute 2
Synonyms	AG02,AGO 2,Ago-2,Ago2,CG13452,CG7439,Dm Ago2,DmAgo2,Dmel\CG7439,ago,ago-2,ago2,dAGO2,dAgo2,eIF2C2
General protein information	Preferred Names Argonaute 2 Names CG7439 gene product from transcript CG7439-RC AGO2-PB AGO2-PC AGO2-PE ARGONAUTE2 CG7439-PB CG7439-PC CG7439-PE argonaute argonaute 2 argonaute-2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 71C3-71E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_140518.3	NP_648775.1	argonaute 2, isoform B
NM_001274953.1	NP_001261882.1	argonaute 2, isoform E
NM_168626.3	NP_730054.1	argonaute 2, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	AGO2	NP_648775.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011537	XP_559969.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutation of T1149 or R1158 in the conserved PIWI domain causes AGO2 protein instability, but only T1149 affects RNAi activity. Mass spec analysis shows that several proteasome components co-purify with both wildtype and mutant AGO2, and knockdown of two proteasome pathway components results in AGO2 protein accumulation.
Title: Drosophila Argonaute2 turnover is regulated by the ubiquitin proteasome pathway.

We speculate that the repeated evolution of testis specificity in obscura group Ago2 genes, combined with their dynamic turnover and strong signatures of adaptive evolution, may be associated with highly derived roles in the suppression of transposable elements or meiotic drive
Title: Repeated Duplication of Argonaute2 Is Associated with Strong Selection and Testis Specialization in Drosophila.

Molecular evolution of AGO2 glutamine-rich repeat region
Title: Variation and Evolution in the Glutamine-Rich Repeat Region of Drosophila Argonaute-2.

We find there are large differences in evolutionary rates and gene turnover between pathways, and that paralogs of Ago2, Ago3, and Piwi/Aub show contrasting rates of evolution after duplication.
Title: Duplication and Diversification of Dipteran Argonaute Genes, and the Evolutionary Divergence of Piwi and Aubergine.

Study shows that the Cricket Paralysis virus suppressor of RNA silencing, CrPV-1A but not B2 strongly interfere with the Ago-2-dependent miRNA silencing in Drosophila.
Title: The cricket paralysis virus suppressor inhibits microRNA silencing mediated by the Drosophila Argonaute-2 protein.

The following AGO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa20038	NM_001274953.1 Latest version!	Drosophila melanogaster argonaute 2, transcript variant E (AGO2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa18553	NM_168626.3 Latest version!	Drosophila melanogaster argonaute 2, transcript variant C (AGO2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa18554	NM_140518.3 Latest version!	Drosophila melanogaster argonaute 2, transcript variant B (AGO2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGO2 ( NM_001274953.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261882.1 Drosophila melanogaster argonaute 2, transcript variant E (AGO2), mRNA.

Price & Availability↑

CloneID	OFa20038
Clone ID Related Accession (Same CDS sequence)	NM_001274953.1
Accession Version	NM_001274953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	argonaute 2, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTTCTG TGGCATACCA CTATGACGTG AAGATCATGC CAGAGCGTCC CAAAAAGTTT 
TACAGACAGG CATTTGAACA ATTTCGGGTG GACCAATTGG GCGGAGCAGT TCTTGCCTAT 
GATGGCAAAG CCTCTTGCTA CTCGGTGGAT AAGCTGCCGT TAAATAGCCA AAATCCGGAA 
GTGACTGTGA CAGATCGTAA TGGTCGTACT TTGCGCTACA CGATCGAAAT CAAGGAGACT 
GGTGACTCGA CCATTGACCT TAAATCGTTA ACTACTTATA TGAATGACCG AATTTTCGAC 
AAGCCCATGC GAGCAATGCA GTGCGTGGAG GTTGTTTTGG CTTCACCCTG CCACAACAAA 
GCCATCCGTG TCGGTCGTTC CTTCTTTAAG ATGTCCGATC CTAATAATCG TCACGAACTG 
GATGATGGAT ACGAGGCCCT GGTCGGTCTG TATCAGGCAT TTATGCTGGG CGATAGGCCA 
TTTTTAAATG TAGATATATC GCACAAATCC TTTCCGATTT CAATGCCGAT GATCGAATAT 
TTGGAGCGAT TTAGCTTAAA GGCTAAAATC AACAATACAA CGAATTTAGA TTATTCGCGA 
CGCTTCCTTG AGCCATTCCT CAGAGGCATT AATGTGGTTT ACACGCCTCC TCAGTCGTTT 
CAAAGCGCTC CAAGGGTATA CCGTGTAAAT GGTCTTTCTC GTGCCCCGGC CAGCAGTGAG 
ACCTTCGAAC ACGATGGGAA GAAGGTCACC ATTGCGTCCT ACTTCCACAG TCGCAACTAC 
CCATTGAAGT TTCCCCAACT CCATTGTCTG AACGTTGGAT CTTCAATCAA GAGTATTCTG 
CTGCCCATCG AGCTATGCAG CATCGAGGAA GGTCAGGCCC TAAACCGCAA GGATGGAGCA 
ACTCAGGTGG CCAATATGAT AAAGTACGCA GCCACATCGA CGAACGTGCG AAAGCGCAAG 
ATTATGAACT TGCTGCAATA CTTCCAGCAC AACCTGGATC CGACCATCAG TCGCTTTGGC 
ATCCGCATTG CCAACGATTT TATTGTGGTA AGCACCCGCG TCCTAAGCCC ACCTCAGGTT 
GAATATCATA GTAAGAGGTT TACTATGGTG AAGAACGGGT CGTGGCGCAT GGATGGCATG 
AAGTTTCTGG AGCCCAAGCC CAAGGCGCAC AAGTGTGCGG TCTTGTATTG CGATCCGAGG 
AGTGGTCGCA AAATGAACTA TACCCAGCTG AATGACTTCG GGAACCTAAT AATATCCCAA 
GGCAAGGCAG TCAACATAAG CTTGGATTCT GATGTGACAT ACAGACCGTT CACGGATGAC 
GAACGCAGCC TAGACACTAT TTTCGCGGAT CTGAAGCGCA GCCAGCACGA TCTGGCAATT 
GTGATTATTC CTCAGTTTAG AATTTCTTAC GATACAATTA AGCAGAAGGC CGAGCTGCAG 
CATGGAATTT TGACGCAATG CATTAAGCAG TTCACCGTGG AACGAAAGTG TAATAATCAG 
ACGATTGGAA ATATTCTACT TAAGATCAAC TCCAAGCTGA ACGGCATCAA CCACAAGATC 
AAGGATGATC CTCGTCTGCC GATGATGAAG AACACCATGT ACATTGGAGC CGATGTGACC 
CATCCCTCTC CCGATCAGCG CGAGATTCCC AGTGTGGTCG GAGTAGCAGC CTCACACGAT 
CCCTACGGAG CCAGTTATAA CATGCAATAT CGTTTGCAGC GAGGGGCTCT GGAGGAGATT 
GAGGACATGT TCTCGATTAC TTTGGAGCAC TTGCGCGTGT ATAAAGAGTA CCGTAACGCC 
TATCCTGATC ATATCATCTA CTACCGAGAT GGCGTGAGCG ACGGCCAGTT TCCGAAAATC 
AAAAACGAGG AACTGAGGTG TATTAAACAA GCCTGTGACA AGGTGGGCTG TAAACCCAAG 
ATTTGCTGCG TGATTGTGGT GAAGCGTCAT CACACTCGCT TCTTTCCCAG CGGCGACGTA 
ACGACATCGA ACAAGTTCAA CAACGTGGAC CCCGGAACCG TGGTCGATCG CACCATTGTG 
CATCCTAACG AGATGCAGTT CTTCATGGTC AGCCACCAGG CCATCCAGGG CACGGCCAAG 
CCAACACGAT ACAATGTGAT TGAGAACACA GGCAATCTTG ACATCGACTT GTTGCAGCAG 
TTGACCTACA ACCTGTGCCA CATGTTCCCT CGTTGCAATC GCTCGGTTTC TTATCCGGCT 
CCGGCCTATT TAGCCCATTT GGTAGCTGCT CGTGGACGCG TTTATCTGAC TGGCACCAAC 
AGGTTCCTGG ATTTGAAGAA GGAGTACGCA AAGCGAACGA TTGTCCCCGA ATTCATGAAG 
AAAAACCCCA TGTACTTTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261882.1
CDS	1110..3473
Translation	MPSVAYHYDVKIMPERPKKFYRQAFEQFRVDQLGGAVLAYDGKASCYSVDKLPLNSQNPEVTVTDRNGRTLRYTIEIKETGDSTIDLKSLTTYMNDRIFDKPMRAMQCVEVVLASPCHNKAIRVGRSFFKMSDPNNRHELDDGYEALVGLYQAFMLGDRPFLNVDISHKSFPISMPMIEYLERFSLKAKINNTTNLDYSRRFLEPFLRGINVVYTPPQSFQSAPRVYRVNGLSRAPASSETFEHDGKKVTIASYFHSRNYPLKFPQLHCLNVGSSIKSILLPIELCSIEEGQALNRKDGATQVANMIKYAATSTNVRKRKIMNLLQYFQHNLDPTISRFGIRIANDFIVVSTRVLSPPQVEYHSKRFTMVKNGSWRMDGMKFLEPKPKAHKCAVLYCDPRSGRKMNYTQLNDFGNLIISQGKAVNISLDSDVTYRPFTDDERSLDTIFADLKRSQHDLAIVIIPQFRISYDTIKQKAELQHGILTQCIKQFTVERKCNNQTIGNILLKINSKLNGINHKIKDDPRLPMMKNTMYIGADVTHPSPDQREIPSVVGVAASHDPYGASYNMQYRLQRGALEEIEDMFSITLEHLRVYKEYRNAYPDHIIYYRDGVSDGQFPKIKNEELRCIKQACDKVGCKPKICCVIVVKRHHTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMVSHQAIQGTAKPTRYNVIENTGNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLAHLVAARGRVYLTGTNRFLDLKKEYAKRTIVPEFMKKNPMYFV

Target ORF information:

RefSeq Version	NM_001274953.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster argonaute 2, transcript variant E (AGO2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274953.1

ORF Insert Sequence:

ATGCCTTCTG TGGCATACCA CTATGACGTG AAGATCATGC CAGAGCGTCC CAAAAAGTTT 
TACAGACAGG CATTTGAACA ATTTCGGGTG GACCAATTGG GCGGAGCAGT TCTTGCCTAT 
GATGGCAAAG CCTCTTGCTA CTCGGTGGAT AAGCTGCCGT TAAATAGCCA AAATCCGGAA 
GTGACTGTGA CAGATCGTAA TGGTCGTACT TTGCGCTACA CGATCGAAAT CAAGGAGACT 
GGTGACTCGA CCATTGACCT TAAATCGTTA ACTACTTATA TGAATGACCG AATTTTCGAC 
AAGCCCATGC GAGCAATGCA GTGCGTGGAG GTTGTTTTGG CTTCACCCTG CCACAACAAA 
GCCATCCGTG TCGGTCGTTC CTTCTTTAAG ATGTCCGATC CTAATAATCG TCACGAACTG 
GATGATGGAT ACGAGGCCCT GGTCGGTCTG TATCAGGCAT TTATGCTGGG CGATAGGCCA 
TTTTTAAATG TAGATATATC GCACAAATCC TTTCCGATTT CAATGCCGAT GATCGAATAT 
TTGGAGCGAT TTAGCTTAAA GGCTAAAATC AACAATACAA CGAATTTAGA TTATTCGCGA 
CGCTTCCTTG AGCCATTCCT CAGAGGCATT AATGTGGTTT ACACGCCTCC TCAGTCGTTT 
CAAAGCGCTC CAAGGGTATA CCGTGTAAAT GGTCTTTCTC GTGCCCCGGC CAGCAGTGAG 
ACCTTCGAAC ACGATGGGAA GAAGGTCACC ATTGCGTCCT ACTTCCACAG TCGCAACTAC 
CCATTGAAGT TTCCCCAACT CCATTGTCTG AACGTTGGAT CTTCAATCAA GAGTATTCTG 
CTGCCCATCG AGCTATGCAG CATCGAGGAA GGTCAGGCCC TAAACCGCAA GGATGGAGCA 
ACTCAGGTGG CCAATATGAT AAAGTACGCA GCCACATCGA CGAACGTGCG AAAGCGCAAG 
ATTATGAACT TGCTGCAATA CTTCCAGCAC AACCTGGATC CGACCATCAG TCGCTTTGGC 
ATCCGCATTG CCAACGATTT TATTGTGGTA AGCACCCGCG TCCTAAGCCC ACCTCAGGTT 
GAATATCATA GTAAGAGGTT TACTATGGTG AAGAACGGGT CGTGGCGCAT GGATGGCATG 
AAGTTTCTGG AGCCCAAGCC CAAGGCGCAC AAGTGTGCGG TCTTGTATTG CGATCCGAGG 
AGTGGTCGCA AAATGAACTA TACCCAGCTG AATGACTTCG GGAACCTAAT AATATCCCAA 
GGCAAGGCAG TCAACATAAG CTTGGATTCT GATGTGACAT ACAGACCGTT CACGGATGAC 
GAACGCAGCC TAGACACTAT TTTCGCGGAT CTGAAGCGCA GCCAGCACGA TCTGGCAATT 
GTGATTATTC CTCAGTTTAG AATTTCTTAC GATACAATTA AGCAGAAGGC CGAGCTGCAG 
CATGGAATTT TGACGCAATG CATTAAGCAG TTCACCGTGG AACGAAAGTG TAATAATCAG 
ACGATTGGAA ATATTCTACT TAAGATCAAC TCCAAGCTGA ACGGCATCAA CCACAAGATC 
AAGGATGATC CTCGTCTGCC GATGATGAAG AACACCATGT ACATTGGAGC CGATGTGACC 
CATCCCTCTC CCGATCAGCG CGAGATTCCC AGTGTGGTCG GAGTAGCAGC CTCACACGAT 
CCCTACGGAG CCAGTTATAA CATGCAATAT CGTTTGCAGC GAGGGGCTCT GGAGGAGATT 
GAGGACATGT TCTCGATTAC TTTGGAGCAC TTGCGCGTGT ATAAAGAGTA CCGTAACGCC 
TATCCTGATC ATATCATCTA CTACCGAGAT GGCGTGAGCG ACGGCCAGTT TCCGAAAATC 
AAAAACGAGG AACTGAGGTG TATTAAACAA GCCTGTGACA AGGTGGGCTG TAAACCCAAG 
ATTTGCTGCG TGATTGTGGT GAAGCGTCAT CACACTCGCT TCTTTCCCAG CGGCGACGTA 
ACGACATCGA ACAAGTTCAA CAACGTGGAC CCCGGAACCG TGGTCGATCG CACCATTGTG 
CATCCTAACG AGATGCAGTT CTTCATGGTC AGCCACCAGG CCATCCAGGG CACGGCCAAG 
CCAACACGAT ACAATGTGAT TGAGAACACA GGCAATCTTG ACATCGACTT GTTGCAGCAG 
TTGACCTACA ACCTGTGCCA CATGTTCCCT CGTTGCAATC GCTCGGTTTC TTATCCGGCT 
CCGGCCTATT TAGCCCATTT GGTAGCTGCT CGTGGACGCG TTTATCTGAC TGGCACCAAC 
AGGTTCCTGG ATTTGAAGAA GGAGTACGCA AAGCGAACGA TTGTCCCCGA ATTCATGAAG 
AAAAACCCCA TGTACTTTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO2 ( NM_168626.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730054.1 Drosophila melanogaster argonaute 2, transcript variant C (AGO2), mRNA.

Price & Availability↑

CloneID	OFa18553
Clone ID Related Accession (Same CDS sequence)	NM_168626.3
Accession Version	NM_168626.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	argonaute 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168626.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACTTTC CAATTACCAC CCCAGAGAAC AAGAAAGGAG GACAGGATAG CGCTGCAGCA 
CCACAGCCCC AGCAGCAGCA AAAGCAACAA CAACAACGGC AGCAACAACC ACAGCAGCTG 
CAACAACCAC AGCAGCTGCA ACAACCACAG CAGCTGCAAC AACCACAGCA GCAGCAACAA 
CAACAACCGC ATCAGCAACA ACAACAAAGT TCGAGACAAC AGCCATCCAC AAGCTCAGGC 
GGATCCCGTG CATCTGGGTT CCAGCAAGGA GGCCAACAGC AAAAATCCCA AGACGCAGAA 
GGATGGACTG CACAGAAAAA ACAAGGCAAA CAGCAGGTAC AAGGGTGGAC TAAACAAGGT 
CAACAAGGTG GCCATCAGCA AGGACGACAA GGGCAAGACG GTGGCTACCA ACAGCGTCCG 
CCTGGACAAC AACAAGGTGG CCATCAGCAA GGACGACAAG GGCAAGAAGG TGGCTACCAA 
CAGCGTCCGC CTGGACAACA ACAAGGTGGC CATCAGCAAG GACGACAAGG GCAAGAAGGT 
GGCTACCAAC AGCGTCCGTC TGGACAACAA CAAGGTGGCC ATCAGCAAGG ACGACAAGGG 
CAAGAAGGTG GCTACCAACA GCGTCCGCCT GGACAACAAC AAGGTGGCCA TCAGCAAGGA 
CGACAAGGGC AAGAAGGTGG CTACCAACAG CGTCCGTCTG GACAACAACA AGGTGGCCAT 
CAGCAAGGAC GACAAGGGCA AGAAGGTGGC TACCAACAGC GTCCGCCTGG ACAACAACAA 
GGTGGCCATC AGCAAGGACG ACAAGGGCAA GAAGGTGGCT ACCAACAGCG TCCGCCTGGA 
CAACAACAAG GTGGCCATGA GCAAGGACGA CAAGGGCAAG AAGGTGGCTA CCAACAGCGT 
CCGTCTGGAC AACAACAAGG TGGCCATCAG CAAGGACGAC AAGGGCAAGA AGGTGGCTAC 
CAACAGCGTC CGTCTGGACA ACAACAAGGT GGCCATCAGC AAGGACGACA AGGGCAAGAA 
GGTGGCTACC AACAGCGTCC GTCTGGACAA CAACAAGGTG GCCATCAGCA AGGACGACAA 
GGGCAAGAAG GTGGCTACCA ACAGCGTCCG CCTGGACAAC AACCAAACCA AACCCAGAGC 
CAAGGCCAAT ACCAATCTCG TGGACCACCT CAGCAACAGC AGGCTGCCCC ATTACCATTA 
CCGCCTCAGC CAGCCGGGAG CATTAAGCGC GGAACAATCG GCAAACCCGG GCAAGTAGGC 
ATCAACTATC TGGACCTTGA CCTGTCCAAA ATGCCTTCTG TGGCATACCA CTATGACGTG 
AAGATCATGC CAGAGCGTCC CAAAAAGTTT TACAGACAGG CATTTGAACA ATTTCGGGTG 
GACCAATTGG GCGGAGCAGT TCTTGCCTAT GATGGCAAAG CCTCTTGCTA CTCGGTGGAT 
AAGCTGCCGT TAAATAGCCA AAATCCGGAA GTGACTGTGA CAGATCGTAA TGGTCGTACT 
TTGCGCTACA CGATCGAAAT CAAGGAGACT GGTGACTCGA CCATTGACCT TAAATCGTTA 
ACTACTTATA TGAATGACCG AATTTTCGAC AAGCCCATGC GAGCAATGCA GTGCGTGGAG 
GTTGTTTTGG CTTCACCCTG CCACAACAAA GCCATCCGTG TCGGTCGTTC CTTCTTTAAG 
ATGTCCGATC CTAATAATCG TCACGAACTG GATGATGGAT ACGAGGCCCT GGTCGGTCTG 
TATCAGGCAT TTATGCTGGG CGATAGGCCA TTTTTAAATG TAGATATATC GCACAAATCC 
TTTCCGATTT CAATGCCGAT GATCGAATAT TTGGAGCGAT TTAGCTTAAA GGCTAAAATC 
AACAATACAA CGAATTTAGA TTATTCGCGA CGCTTCCTTG AGCCATTCCT CAGAGGCATT 
AATGTGGTTT ACACGCCTCC TCAGTCGTTT CAAAGCGCTC CAAGGGTATA CCGTGTAAAT 
GGTCTTTCTC GTGCCCCGGC CAGCAGTGAG ACCTTCGAAC ACGATGGGAA GAAGGTCACC 
ATTGCGTCCT ACTTCCACAG TCGCAACTAC CCATTGAAGT TTCCCCAACT CCATTGTCTG 
AACGTTGGAT CTTCAATCAA GAGTATTCTG CTGCCCATCG AGCTATGCAG CATCGAGGAA 
GGTCAGGCCC TAAACCGCAA GGATGGAGCA ACTCAGGTGG CCAATATGAT AAAGTACGCA 
GCCACATCGA CGAACGTGCG AAAGCGCAAG ATTATGAACT TGCTGCAATA CTTCCAGCAC 
AACCTGGATC CGACCATCAG TCGCTTTGGC ATCCGCATTG CCAACGATTT TATTGTGGTA 
AGCACCCGCG TCCTAAGCCC ACCTCAGGTT GAATATCATA GTAAGAGGTT TACTATGGTG 
AAGAACGGGT CGTGGCGCAT GGATGGCATG AAGTTTCTGG AGCCCAAGCC CAAGGCGCAC 
AAGTGTGCGG TCTTGTATTG CGATCCGAGG AGTGGTCGCA AAATGAACTA TACCCAGCTG 
AATGACTTCG GGAACCTAAT AATATCCCAA GGCAAGGCAG TCAACATAAG CTTGGATTCT 
GATGTGACAT ACAGACCGTT CACGGATGAC GAACGCAGCC TAGACACTAT TTTCGCGGAT 
CTGAAGCGCA GCCAGCACGA TCTGGCAATT GTGATTATTC CTCAGTTTAG AATTTCTTAC 
GATACAATTA AGCAGAAGGC CGAGCTGCAG CATGGAATTT TGACGCAATG CATTAAGCAG 
TTCACCGTGG AACGAAAGTG TAATAATCAG ACGATTGGAA ATATTCTACT TAAGATCAAC 
TCCAAGCTGA ACGGCATCAA CCACAAGATC AAGGATGATC CTCGTCTGCC GATGATGAAG 
AACACCATGT ACATTGGAGC CGATGTGACC CATCCCTCTC CCGATCAGCG CGAGATTCCC 
AGTGTGGTCG GAGTAGCAGC CTCACACGAT CCCTACGGAG CCAGTTATAA CATGCAATAT 
CGTTTGCAGC GAGGGGCTCT GGAGGAGATT GAGGACATGT TCTCGATTAC TTTGGAGCAC 
TTGCGCGTGT ATAAAGAGTA CCGTAACGCC TATCCTGATC ATATCATCTA CTACCGAGAT 
GGCGTGAGCG ACGGCCAGTT TCCGAAAATC AAAAACGAGG AACTGAGGTG TATTAAACAA 
GCCTGTGACA AGGTGGGCTG TAAACCCAAG ATTTGCTGCG TGATTGTGGT GAAGCGTCAT 
CACACTCGCT TCTTTCCCAG CGGCGACGTA ACGACATCGA ACAAGTTCAA CAACGTGGAC 
CCCGGAACCG TGGTCGATCG CACCATTGTG CATCCTAACG AGATGCAGTT CTTCATGGTC 
AGCCACCAGG CCATCCAGGG CACGGCCAAG CCAACACGAT ACAATGTGAT TGAGAACACA 
GGCAATCTTG ACATCGACTT GTTGCAGCAG TTGACCTACA ACCTGTGCCA CATGTTCCCT 
CGTTGCAATC GCTCGGTTTC TTATCCGGCT CCGGCCTATT TAGCCCATTT GGTAGCTGCT 
CGTGGACGCG TTTATCTGAC TGGCACCAAC AGGTTCCTGG ATTTGAAGAA GGAGTACGCA 
AAGCGAACGA TTGTCCCCGA ATTCATGAAG AAAAACCCCA TGTACTTTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730054.1
CDS	153..3806
Translation	MHFPITTPENKKGGQDSAAAPQPQQQQKQQQQRQQQPQQLQQPQQLQQPQQLQQPQQQQQQQPHQQQQQSSRQQPSTSSGGSRASGFQQGGQQQKSQDAEGWTAQKKQGKQQVQGWTKQGQQGGHQQGRQGQDGGYQQRPPGQQQGGHQQGRQGQEGGYQQRPPGQQQGGHQQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPPGQQQGGHQQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPPGQQQGGHQQGRQGQEGGYQQRPPGQQQGGHEQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPPGQQPNQTQSQGQYQSRGPPQQQQAAPLPLPPQPAGSIKRGTIGKPGQVGINYLDLDLSKMPSVAYHYDVKIMPERPKKFYRQAFEQFRVDQLGGAVLAYDGKASCYSVDKLPLNSQNPEVTVTDRNGRTLRYTIEIKETGDSTIDLKSLTTYMNDRIFDKPMRAMQCVEVVLASPCHNKAIRVGRSFFKMSDPNNRHELDDGYEALVGLYQAFMLGDRPFLNVDISHKSFPISMPMIEYLERFSLKAKINNTTNLDYSRRFLEPFLRGINVVYTPPQSFQSAPRVYRVNGLSRAPASSETFEHDGKKVTIASYFHSRNYPLKFPQLHCLNVGSSIKSILLPIELCSIEEGQALNRKDGATQVANMIKYAATSTNVRKRKIMNLLQYFQHNLDPTISRFGIRIANDFIVVSTRVLSPPQVEYHSKRFTMVKNGSWRMDGMKFLEPKPKAHKCAVLYCDPRSGRKMNYTQLNDFGNLIISQGKAVNISLDSDVTYRPFTDDERSLDTIFADLKRSQHDLAIVIIPQFRISYDTIKQKAELQHGILTQCIKQFTVERKCNNQTIGNILLKINSKLNGINHKIKDDPRLPMMKNTMYIGADVTHPSPDQREIPSVVGVAASHDPYGASYNMQYRLQRGALEEIEDMFSITLEHLRVYKEYRNAYPDHIIYYRDGVSDGQFPKIKNEELRCIKQACDKVGCKPKICCVIVVKRHHTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMVSHQAIQGTAKPTRYNVIENTGNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLAHLVAARGRVYLTGTNRFLDLKKEYAKRTIVPEFMKKNPMYFV

Target ORF information:

RefSeq Version	NM_168626.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster argonaute 2, transcript variant C (AGO2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168626.3

ORF Insert Sequence:

ATGCACTTTC CAATTACCAC CCCAGAGAAC AAGAAAGGAG GACAGGATAG CGCTGCAGCA 
CCACAGCCCC AGCAGCAGCA AAAGCAACAA CAACAACGGC AGCAACAACC ACAGCAGCTG 
CAACAACCAC AGCAGCTGCA ACAACCACAG CAGCTGCAAC AACCACAGCA GCAGCAACAA 
CAACAACCGC ATCAGCAACA ACAACAAAGT TCGAGACAAC AGCCATCCAC AAGCTCAGGC 
GGATCCCGTG CATCTGGGTT CCAGCAAGGA GGCCAACAGC AAAAATCCCA AGACGCAGAA 
GGATGGACTG CACAGAAAAA ACAAGGCAAA CAGCAGGTAC AAGGGTGGAC TAAACAAGGT 
CAACAAGGTG GCCATCAGCA AGGACGACAA GGGCAAGACG GTGGCTACCA ACAGCGTCCG 
CCTGGACAAC AACAAGGTGG CCATCAGCAA GGACGACAAG GGCAAGAAGG TGGCTACCAA 
CAGCGTCCGC CTGGACAACA ACAAGGTGGC CATCAGCAAG GACGACAAGG GCAAGAAGGT 
GGCTACCAAC AGCGTCCGTC TGGACAACAA CAAGGTGGCC ATCAGCAAGG ACGACAAGGG 
CAAGAAGGTG GCTACCAACA GCGTCCGCCT GGACAACAAC AAGGTGGCCA TCAGCAAGGA 
CGACAAGGGC AAGAAGGTGG CTACCAACAG CGTCCGTCTG GACAACAACA AGGTGGCCAT 
CAGCAAGGAC GACAAGGGCA AGAAGGTGGC TACCAACAGC GTCCGCCTGG ACAACAACAA 
GGTGGCCATC AGCAAGGACG ACAAGGGCAA GAAGGTGGCT ACCAACAGCG TCCGCCTGGA 
CAACAACAAG GTGGCCATGA GCAAGGACGA CAAGGGCAAG AAGGTGGCTA CCAACAGCGT 
CCGTCTGGAC AACAACAAGG TGGCCATCAG CAAGGACGAC AAGGGCAAGA AGGTGGCTAC 
CAACAGCGTC CGTCTGGACA ACAACAAGGT GGCCATCAGC AAGGACGACA AGGGCAAGAA 
GGTGGCTACC AACAGCGTCC GTCTGGACAA CAACAAGGTG GCCATCAGCA AGGACGACAA 
GGGCAAGAAG GTGGCTACCA ACAGCGTCCG CCTGGACAAC AACCAAACCA AACCCAGAGC 
CAAGGCCAAT ACCAATCTCG TGGACCACCT CAGCAACAGC AGGCTGCCCC ATTACCATTA 
CCGCCTCAGC CAGCCGGGAG CATTAAGCGC GGAACAATCG GCAAACCCGG GCAAGTAGGC 
ATCAACTATC TGGACCTTGA CCTGTCCAAA ATGCCTTCTG TGGCATACCA CTATGACGTG 
AAGATCATGC CAGAGCGTCC CAAAAAGTTT TACAGACAGG CATTTGAACA ATTTCGGGTG 
GACCAATTGG GCGGAGCAGT TCTTGCCTAT GATGGCAAAG CCTCTTGCTA CTCGGTGGAT 
AAGCTGCCGT TAAATAGCCA AAATCCGGAA GTGACTGTGA CAGATCGTAA TGGTCGTACT 
TTGCGCTACA CGATCGAAAT CAAGGAGACT GGTGACTCGA CCATTGACCT TAAATCGTTA 
ACTACTTATA TGAATGACCG AATTTTCGAC AAGCCCATGC GAGCAATGCA GTGCGTGGAG 
GTTGTTTTGG CTTCACCCTG CCACAACAAA GCCATCCGTG TCGGTCGTTC CTTCTTTAAG 
ATGTCCGATC CTAATAATCG TCACGAACTG GATGATGGAT ACGAGGCCCT GGTCGGTCTG 
TATCAGGCAT TTATGCTGGG CGATAGGCCA TTTTTAAATG TAGATATATC GCACAAATCC 
TTTCCGATTT CAATGCCGAT GATCGAATAT TTGGAGCGAT TTAGCTTAAA GGCTAAAATC 
AACAATACAA CGAATTTAGA TTATTCGCGA CGCTTCCTTG AGCCATTCCT CAGAGGCATT 
AATGTGGTTT ACACGCCTCC TCAGTCGTTT CAAAGCGCTC CAAGGGTATA CCGTGTAAAT 
GGTCTTTCTC GTGCCCCGGC CAGCAGTGAG ACCTTCGAAC ACGATGGGAA GAAGGTCACC 
ATTGCGTCCT ACTTCCACAG TCGCAACTAC CCATTGAAGT TTCCCCAACT CCATTGTCTG 
AACGTTGGAT CTTCAATCAA GAGTATTCTG CTGCCCATCG AGCTATGCAG CATCGAGGAA 
GGTCAGGCCC TAAACCGCAA GGATGGAGCA ACTCAGGTGG CCAATATGAT AAAGTACGCA 
GCCACATCGA CGAACGTGCG AAAGCGCAAG ATTATGAACT TGCTGCAATA CTTCCAGCAC 
AACCTGGATC CGACCATCAG TCGCTTTGGC ATCCGCATTG CCAACGATTT TATTGTGGTA 
AGCACCCGCG TCCTAAGCCC ACCTCAGGTT GAATATCATA GTAAGAGGTT TACTATGGTG 
AAGAACGGGT CGTGGCGCAT GGATGGCATG AAGTTTCTGG AGCCCAAGCC CAAGGCGCAC 
AAGTGTGCGG TCTTGTATTG CGATCCGAGG AGTGGTCGCA AAATGAACTA TACCCAGCTG 
AATGACTTCG GGAACCTAAT AATATCCCAA GGCAAGGCAG TCAACATAAG CTTGGATTCT 
GATGTGACAT ACAGACCGTT CACGGATGAC GAACGCAGCC TAGACACTAT TTTCGCGGAT 
CTGAAGCGCA GCCAGCACGA TCTGGCAATT GTGATTATTC CTCAGTTTAG AATTTCTTAC 
GATACAATTA AGCAGAAGGC CGAGCTGCAG CATGGAATTT TGACGCAATG CATTAAGCAG 
TTCACCGTGG AACGAAAGTG TAATAATCAG ACGATTGGAA ATATTCTACT TAAGATCAAC 
TCCAAGCTGA ACGGCATCAA CCACAAGATC AAGGATGATC CTCGTCTGCC GATGATGAAG 
AACACCATGT ACATTGGAGC CGATGTGACC CATCCCTCTC CCGATCAGCG CGAGATTCCC 
AGTGTGGTCG GAGTAGCAGC CTCACACGAT CCCTACGGAG CCAGTTATAA CATGCAATAT 
CGTTTGCAGC GAGGGGCTCT GGAGGAGATT GAGGACATGT TCTCGATTAC TTTGGAGCAC 
TTGCGCGTGT ATAAAGAGTA CCGTAACGCC TATCCTGATC ATATCATCTA CTACCGAGAT 
GGCGTGAGCG ACGGCCAGTT TCCGAAAATC AAAAACGAGG AACTGAGGTG TATTAAACAA 
GCCTGTGACA AGGTGGGCTG TAAACCCAAG ATTTGCTGCG TGATTGTGGT GAAGCGTCAT 
CACACTCGCT TCTTTCCCAG CGGCGACGTA ACGACATCGA ACAAGTTCAA CAACGTGGAC 
CCCGGAACCG TGGTCGATCG CACCATTGTG CATCCTAACG AGATGCAGTT CTTCATGGTC 
AGCCACCAGG CCATCCAGGG CACGGCCAAG CCAACACGAT ACAATGTGAT TGAGAACACA 
GGCAATCTTG ACATCGACTT GTTGCAGCAG TTGACCTACA ACCTGTGCCA CATGTTCCCT 
CGTTGCAATC GCTCGGTTTC TTATCCGGCT CCGGCCTATT TAGCCCATTT GGTAGCTGCT 
CGTGGACGCG TTTATCTGAC TGGCACCAAC AGGTTCCTGG ATTTGAAGAA GGAGTACGCA 
AAGCGAACGA TTGTCCCCGA ATTCATGAAG AAAAACCCCA TGTACTTTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO2 ( NM_140518.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648775.1 Drosophila melanogaster argonaute 2, transcript variant B (AGO2), mRNA.

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CloneID	OFa18554
Clone ID Related Accession (Same CDS sequence)	NM_140518.3
Accession Version	NM_140518.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	argonaute 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140518.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAAAA AAGATAAGAA CAAGAAAGGA GGACAGGATA GCGCTGCAGC ACCACAGCCC 
CAGCAGCAGC AAAAGCAACA ACAACAACGG CAGCAACAAC CACAGCAGCT GCAACAACCA 
CAGCAGCTGC AACAACCACA GCAGCTGCAA CAACCACAGC AGCAGCAACA ACAACAACCG 
CATCAGCAAC AACAACAAAG TTCGAGACAA CAGCCATCCA CAAGCTCAGG CGGATCCCGT 
GCATCTGGGT TCCAGCAAGG AGGCCAACAG CAAAAATCCC AAGACGCAGA AGGATGGACT 
GCACAGAAAA AACAAGGCAA ACAGCAGGTA CAAGGGTGGA CTAAACAAGG TCAACAAGGT 
GGCCATCAGC AAGGACGACA AGGGCAAGAC GGTGGCTACC AACAGCGTCC GCCTGGACAA 
CAACAAGGTG GCCATCAGCA AGGACGACAA GGGCAAGAAG GTGGCTACCA ACAGCGTCCG 
CCTGGACAAC AACAAGGTGG CCATCAGCAA GGACGACAAG GGCAAGAAGG TGGCTACCAA 
CAGCGTCCGT CTGGACAACA ACAAGGTGGC CATCAGCAAG GACGACAAGG GCAAGAAGGT 
GGCTACCAAC AGCGTCCGCC TGGACAACAA CAAGGTGGCC ATCAGCAAGG ACGACAAGGG 
CAAGAAGGTG GCTACCAACA GCGTCCGTCT GGACAACAAC AAGGTGGCCA TCAGCAAGGA 
CGACAAGGGC AAGAAGGTGG CTACCAACAG CGTCCGCCTG GACAACAACA AGGTGGCCAT 
CAGCAAGGAC GACAAGGGCA AGAAGGTGGC TACCAACAGC GTCCGCCTGG ACAACAACAA 
GGTGGCCATG AGCAAGGACG ACAAGGGCAA GAAGGTGGCT ACCAACAGCG TCCGTCTGGA 
CAACAACAAG GTGGCCATCA GCAAGGACGA CAAGGGCAAG AAGGTGGCTA CCAACAGCGT 
CCGTCTGGAC AACAACAAGG TGGCCATCAG CAAGGACGAC AAGGGCAAGA AGGTGGCTAC 
CAACAGCGTC CGTCTGGACA ACAACAAGGT GGCCATCAGC AAGGACGACA AGGGCAAGAA 
GGTGGCTACC AACAGCGTCC GCCTGGACAA CAACCAAACC AAACCCAGAG CCAAGGCCAA 
TACCAATCTC GTGGACCACC TCAGCAACAG CAGGCTGCCC CATTACCATT ACCGCCTCAG 
CCAGCCGGGA GCATTAAGCG CGGAACAATC GGCAAACCCG GGCAAGTAGG CATCAACTAT 
CTGGACCTTG ACCTGTCCAA AATGCCTTCT GTGGCATACC ACTATGACGT GAAGATCATG 
CCAGAGCGTC CCAAAAAGTT TTACAGACAG GCATTTGAAC AATTTCGGGT GGACCAATTG 
GGCGGAGCAG TTCTTGCCTA TGATGGCAAA GCCTCTTGCT ACTCGGTGGA TAAGCTGCCG 
TTAAATAGCC AAAATCCGGA AGTGACTGTG ACAGATCGTA ATGGTCGTAC TTTGCGCTAC 
ACGATCGAAA TCAAGGAGAC TGGTGACTCG ACCATTGACC TTAAATCGTT AACTACTTAT 
ATGAATGACC GAATTTTCGA CAAGCCCATG CGAGCAATGC AGTGCGTGGA GGTTGTTTTG 
GCTTCACCCT GCCACAACAA AGCCATCCGT GTCGGTCGTT CCTTCTTTAA GATGTCCGAT 
CCTAATAATC GTCACGAACT GGATGATGGA TACGAGGCCC TGGTCGGTCT GTATCAGGCA 
TTTATGCTGG GCGATAGGCC ATTTTTAAAT GTAGATATAT CGCACAAATC CTTTCCGATT 
TCAATGCCGA TGATCGAATA TTTGGAGCGA TTTAGCTTAA AGGCTAAAAT CAACAATACA 
ACGAATTTAG ATTATTCGCG ACGCTTCCTT GAGCCATTCC TCAGAGGCAT TAATGTGGTT 
TACACGCCTC CTCAGTCGTT TCAAAGCGCT CCAAGGGTAT ACCGTGTAAA TGGTCTTTCT 
CGTGCCCCGG CCAGCAGTGA GACCTTCGAA CACGATGGGA AGAAGGTCAC CATTGCGTCC 
TACTTCCACA GTCGCAACTA CCCATTGAAG TTTCCCCAAC TCCATTGTCT GAACGTTGGA 
TCTTCAATCA AGAGTATTCT GCTGCCCATC GAGCTATGCA GCATCGAGGA AGGTCAGGCC 
CTAAACCGCA AGGATGGAGC AACTCAGGTG GCCAATATGA TAAAGTACGC AGCCACATCG 
ACGAACGTGC GAAAGCGCAA GATTATGAAC TTGCTGCAAT ACTTCCAGCA CAACCTGGAT 
CCGACCATCA GTCGCTTTGG CATCCGCATT GCCAACGATT TTATTGTGGT AAGCACCCGC 
GTCCTAAGCC CACCTCAGGT TGAATATCAT AGTAAGAGGT TTACTATGGT GAAGAACGGG 
TCGTGGCGCA TGGATGGCAT GAAGTTTCTG GAGCCCAAGC CCAAGGCGCA CAAGTGTGCG 
GTCTTGTATT GCGATCCGAG GAGTGGTCGC AAAATGAACT ATACCCAGCT GAATGACTTC 
GGGAACCTAA TAATATCCCA AGGCAAGGCA GTCAACATAA GCTTGGATTC TGATGTGACA 
TACAGACCGT TCACGGATGA CGAACGCAGC CTAGACACTA TTTTCGCGGA TCTGAAGCGC 
AGCCAGCACG ATCTGGCAAT TGTGATTATT CCTCAGTTTA GAATTTCTTA CGATACAATT 
AAGCAGAAGG CCGAGCTGCA GCATGGAATT TTGACGCAAT GCATTAAGCA GTTCACCGTG 
GAACGAAAGT GTAATAATCA GACGATTGGA AATATTCTAC TTAAGATCAA CTCCAAGCTG 
AACGGCATCA ACCACAAGAT CAAGGATGAT CCTCGTCTGC CGATGATGAA GAACACCATG 
TACATTGGAG CCGATGTGAC CCATCCCTCT CCCGATCAGC GCGAGATTCC CAGTGTGGTC 
GGAGTAGCAG CCTCACACGA TCCCTACGGA GCCAGTTATA ACATGCAATA TCGTTTGCAG 
CGAGGGGCTC TGGAGGAGAT TGAGGACATG TTCTCGATTA CTTTGGAGCA CTTGCGCGTG 
TATAAAGAGT ACCGTAACGC CTATCCTGAT CATATCATCT ACTACCGAGA TGGCGTGAGC 
GACGGCCAGT TTCCGAAAAT CAAAAACGAG GAACTGAGGT GTATTAAACA AGCCTGTGAC 
AAGGTGGGCT GTAAACCCAA GATTTGCTGC GTGATTGTGG TGAAGCGTCA TCACACTCGC 
TTCTTTCCCA GCGGCGACGT AACGACATCG AACAAGTTCA ACAACGTGGA CCCCGGAACC 
GTGGTCGATC GCACCATTGT GCATCCTAAC GAGATGCAGT TCTTCATGGT CAGCCACCAG 
GCCATCCAGG GCACGGCCAA GCCAACACGA TACAATGTGA TTGAGAACAC AGGCAATCTT 
GACATCGACT TGTTGCAGCA GTTGACCTAC AACCTGTGCC ACATGTTCCC TCGTTGCAAT 
CGCTCGGTTT CTTATCCGGC TCCGGCCTAT TTAGCCCATT TGGTAGCTGC TCGTGGACGC 
GTTTATCTGA CTGGCACCAA CAGGTTCCTG GATTTGAAGA AGGAGTACGC AAAGCGAACG 
ATTGTCCCCG AATTCATGAA GAAAAACCCC ATGTACTTTG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648775.1
CDS	95..3739
Translation	MGKKDKNKKGGQDSAAAPQPQQQQKQQQQRQQQPQQLQQPQQLQQPQQLQQPQQQQQQQPHQQQQQSSRQQPSTSSGGSRASGFQQGGQQQKSQDAEGWTAQKKQGKQQVQGWTKQGQQGGHQQGRQGQDGGYQQRPPGQQQGGHQQGRQGQEGGYQQRPPGQQQGGHQQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPPGQQQGGHQQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPPGQQQGGHQQGRQGQEGGYQQRPPGQQQGGHEQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPPGQQPNQTQSQGQYQSRGPPQQQQAAPLPLPPQPAGSIKRGTIGKPGQVGINYLDLDLSKMPSVAYHYDVKIMPERPKKFYRQAFEQFRVDQLGGAVLAYDGKASCYSVDKLPLNSQNPEVTVTDRNGRTLRYTIEIKETGDSTIDLKSLTTYMNDRIFDKPMRAMQCVEVVLASPCHNKAIRVGRSFFKMSDPNNRHELDDGYEALVGLYQAFMLGDRPFLNVDISHKSFPISMPMIEYLERFSLKAKINNTTNLDYSRRFLEPFLRGINVVYTPPQSFQSAPRVYRVNGLSRAPASSETFEHDGKKVTIASYFHSRNYPLKFPQLHCLNVGSSIKSILLPIELCSIEEGQALNRKDGATQVANMIKYAATSTNVRKRKIMNLLQYFQHNLDPTISRFGIRIANDFIVVSTRVLSPPQVEYHSKRFTMVKNGSWRMDGMKFLEPKPKAHKCAVLYCDPRSGRKMNYTQLNDFGNLIISQGKAVNISLDSDVTYRPFTDDERSLDTIFADLKRSQHDLAIVIIPQFRISYDTIKQKAELQHGILTQCIKQFTVERKCNNQTIGNILLKINSKLNGINHKIKDDPRLPMMKNTMYIGADVTHPSPDQREIPSVVGVAASHDPYGASYNMQYRLQRGALEEIEDMFSITLEHLRVYKEYRNAYPDHIIYYRDGVSDGQFPKIKNEELRCIKQACDKVGCKPKICCVIVVKRHHTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMVSHQAIQGTAKPTRYNVIENTGNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLAHLVAARGRVYLTGTNRFLDLKKEYAKRTIVPEFMKKNPMYFV

Target ORF information:

RefSeq Version	NM_140518.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster argonaute 2, transcript variant B (AGO2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140518.3

ORF Insert Sequence:

ATGGGAAAAA AAGATAAGAA CAAGAAAGGA GGACAGGATA GCGCTGCAGC ACCACAGCCC 
CAGCAGCAGC AAAAGCAACA ACAACAACGG CAGCAACAAC CACAGCAGCT GCAACAACCA 
CAGCAGCTGC AACAACCACA GCAGCTGCAA CAACCACAGC AGCAGCAACA ACAACAACCG 
CATCAGCAAC AACAACAAAG TTCGAGACAA CAGCCATCCA CAAGCTCAGG CGGATCCCGT 
GCATCTGGGT TCCAGCAAGG AGGCCAACAG CAAAAATCCC AAGACGCAGA AGGATGGACT 
GCACAGAAAA AACAAGGCAA ACAGCAGGTA CAAGGGTGGA CTAAACAAGG TCAACAAGGT 
GGCCATCAGC AAGGACGACA AGGGCAAGAC GGTGGCTACC AACAGCGTCC GCCTGGACAA 
CAACAAGGTG GCCATCAGCA AGGACGACAA GGGCAAGAAG GTGGCTACCA ACAGCGTCCG 
CCTGGACAAC AACAAGGTGG CCATCAGCAA GGACGACAAG GGCAAGAAGG TGGCTACCAA 
CAGCGTCCGT CTGGACAACA ACAAGGTGGC CATCAGCAAG GACGACAAGG GCAAGAAGGT 
GGCTACCAAC AGCGTCCGCC TGGACAACAA CAAGGTGGCC ATCAGCAAGG ACGACAAGGG 
CAAGAAGGTG GCTACCAACA GCGTCCGTCT GGACAACAAC AAGGTGGCCA TCAGCAAGGA 
CGACAAGGGC AAGAAGGTGG CTACCAACAG CGTCCGCCTG GACAACAACA AGGTGGCCAT 
CAGCAAGGAC GACAAGGGCA AGAAGGTGGC TACCAACAGC GTCCGCCTGG ACAACAACAA 
GGTGGCCATG AGCAAGGACG ACAAGGGCAA GAAGGTGGCT ACCAACAGCG TCCGTCTGGA 
CAACAACAAG GTGGCCATCA GCAAGGACGA CAAGGGCAAG AAGGTGGCTA CCAACAGCGT 
CCGTCTGGAC AACAACAAGG TGGCCATCAG CAAGGACGAC AAGGGCAAGA AGGTGGCTAC 
CAACAGCGTC CGTCTGGACA ACAACAAGGT GGCCATCAGC AAGGACGACA AGGGCAAGAA 
GGTGGCTACC AACAGCGTCC GCCTGGACAA CAACCAAACC AAACCCAGAG CCAAGGCCAA 
TACCAATCTC GTGGACCACC TCAGCAACAG CAGGCTGCCC CATTACCATT ACCGCCTCAG 
CCAGCCGGGA GCATTAAGCG CGGAACAATC GGCAAACCCG GGCAAGTAGG CATCAACTAT 
CTGGACCTTG ACCTGTCCAA AATGCCTTCT GTGGCATACC ACTATGACGT GAAGATCATG 
CCAGAGCGTC CCAAAAAGTT TTACAGACAG GCATTTGAAC AATTTCGGGT GGACCAATTG 
GGCGGAGCAG TTCTTGCCTA TGATGGCAAA GCCTCTTGCT ACTCGGTGGA TAAGCTGCCG 
TTAAATAGCC AAAATCCGGA AGTGACTGTG ACAGATCGTA ATGGTCGTAC TTTGCGCTAC 
ACGATCGAAA TCAAGGAGAC TGGTGACTCG ACCATTGACC TTAAATCGTT AACTACTTAT 
ATGAATGACC GAATTTTCGA CAAGCCCATG CGAGCAATGC AGTGCGTGGA GGTTGTTTTG 
GCTTCACCCT GCCACAACAA AGCCATCCGT GTCGGTCGTT CCTTCTTTAA GATGTCCGAT 
CCTAATAATC GTCACGAACT GGATGATGGA TACGAGGCCC TGGTCGGTCT GTATCAGGCA 
TTTATGCTGG GCGATAGGCC ATTTTTAAAT GTAGATATAT CGCACAAATC CTTTCCGATT 
TCAATGCCGA TGATCGAATA TTTGGAGCGA TTTAGCTTAA AGGCTAAAAT CAACAATACA 
ACGAATTTAG ATTATTCGCG ACGCTTCCTT GAGCCATTCC TCAGAGGCAT TAATGTGGTT 
TACACGCCTC CTCAGTCGTT TCAAAGCGCT CCAAGGGTAT ACCGTGTAAA TGGTCTTTCT 
CGTGCCCCGG CCAGCAGTGA GACCTTCGAA CACGATGGGA AGAAGGTCAC CATTGCGTCC 
TACTTCCACA GTCGCAACTA CCCATTGAAG TTTCCCCAAC TCCATTGTCT GAACGTTGGA 
TCTTCAATCA AGAGTATTCT GCTGCCCATC GAGCTATGCA GCATCGAGGA AGGTCAGGCC 
CTAAACCGCA AGGATGGAGC AACTCAGGTG GCCAATATGA TAAAGTACGC AGCCACATCG 
ACGAACGTGC GAAAGCGCAA GATTATGAAC TTGCTGCAAT ACTTCCAGCA CAACCTGGAT 
CCGACCATCA GTCGCTTTGG CATCCGCATT GCCAACGATT TTATTGTGGT AAGCACCCGC 
GTCCTAAGCC CACCTCAGGT TGAATATCAT AGTAAGAGGT TTACTATGGT GAAGAACGGG 
TCGTGGCGCA TGGATGGCAT GAAGTTTCTG GAGCCCAAGC CCAAGGCGCA CAAGTGTGCG 
GTCTTGTATT GCGATCCGAG GAGTGGTCGC AAAATGAACT ATACCCAGCT GAATGACTTC 
GGGAACCTAA TAATATCCCA AGGCAAGGCA GTCAACATAA GCTTGGATTC TGATGTGACA 
TACAGACCGT TCACGGATGA CGAACGCAGC CTAGACACTA TTTTCGCGGA TCTGAAGCGC 
AGCCAGCACG ATCTGGCAAT TGTGATTATT CCTCAGTTTA GAATTTCTTA CGATACAATT 
AAGCAGAAGG CCGAGCTGCA GCATGGAATT TTGACGCAAT GCATTAAGCA GTTCACCGTG 
GAACGAAAGT GTAATAATCA GACGATTGGA AATATTCTAC TTAAGATCAA CTCCAAGCTG 
AACGGCATCA ACCACAAGAT CAAGGATGAT CCTCGTCTGC CGATGATGAA GAACACCATG 
TACATTGGAG CCGATGTGAC CCATCCCTCT CCCGATCAGC GCGAGATTCC CAGTGTGGTC 
GGAGTAGCAG CCTCACACGA TCCCTACGGA GCCAGTTATA ACATGCAATA TCGTTTGCAG 
CGAGGGGCTC TGGAGGAGAT TGAGGACATG TTCTCGATTA CTTTGGAGCA CTTGCGCGTG 
TATAAAGAGT ACCGTAACGC CTATCCTGAT CATATCATCT ACTACCGAGA TGGCGTGAGC 
GACGGCCAGT TTCCGAAAAT CAAAAACGAG GAACTGAGGT GTATTAAACA AGCCTGTGAC 
AAGGTGGGCT GTAAACCCAA GATTTGCTGC GTGATTGTGG TGAAGCGTCA TCACACTCGC 
TTCTTTCCCA GCGGCGACGT AACGACATCG AACAAGTTCA ACAACGTGGA CCCCGGAACC 
GTGGTCGATC GCACCATTGT GCATCCTAAC GAGATGCAGT TCTTCATGGT CAGCCACCAG 
GCCATCCAGG GCACGGCCAA GCCAACACGA TACAATGTGA TTGAGAACAC AGGCAATCTT 
GACATCGACT TGTTGCAGCA GTTGACCTAC AACCTGTGCC ACATGTTCCC TCGTTGCAAT 
CGCTCGGTTT CTTATCCGGC TCCGGCCTAT TTAGCCCATT TGGTAGCTGC TCGTGGACGC 
GTTTATCTGA CTGGCACCAA CAGGTTCCTG GATTTGAAGA AGGAGTACGC AAAGCGAACG 
ATTGTCCCCG AATTCATGAA GAAAAACCCC ATGTACTTTG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

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AGO2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

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ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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