Reck cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Reck
Entrez Gene ID	39629
Full Name	Reversion-inducing-cysteine-rich protein with kazal motifs
Synonyms	CG5392,Dmel\CG5392,RECK
General protein information	Preferred Names Reversion-inducing-cysteine-rich protein with kazal motifs Names CG5392 gene product from transcript CG5392-RB CG5392-PA CG5392-PB Reck-PA Reck-PB reversion-inducing-cysteine-rich protein with kazal motifs
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 71B1-71B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_140476.3	NP_648733.2	Reversion-inducing-cysteine-rich protein with kazal motifs, isoform A
NM_001274924.1	NP_001261853.1	Reversion-inducing-cysteine-rich protein with kazal motifs, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RECK	XP_520575.2
Mus musculus (house mouse)	Reck	NP_057887.2
Rattus norvegicus (Norway rat)	Reck	NP_001101424.1
Homo sapiens (human)	RECK	NP_066934.1
Canis lupus familiaris (dog)	RECK	NP_001002985.1
Bos taurus (cattle)	RECK	NP_001179394.1
Drosophila melanogaster (fruit fly)	Reck	NP_001261853.1
	RECK	XP_001083599.1
Xenopus tropicalis (tropical clawed frog)	reck	XP_002938983.2
Gallus gallus (chicken)	RECK	XP_418897.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010646	XP_311364.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Notch directly regulates the cell morphogenesis genes Reck, talin and trio in adult muscle progenitor
Title: Notch directly regulates the cell morphogenesis genes Reck, talin and trio in adult muscle progenitors.

The following Reck gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Reck cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18518	NM_001274924.1 Latest version!	Drosophila melanogaster Reversion-inducing-cysteine-rich protein with kazal motifs (Reck), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18518	NM_140476.3 Latest version!	Drosophila melanogaster Reversion-inducing-cysteine-rich protein with kazal motifs (Reck), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Reck ( NM_140476.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648733.2 Drosophila melanogaster Reversion-inducing-cysteine-rich protein with kazal motifs (Reck), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18518
Clone ID Related Accession (Same CDS sequence)	NM_140476.3 , NM_001274924.1
Accession Version	NM_140476.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Reversion-inducing-cysteine-rich protein with kazal motifs, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140476.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCCTGA GTGGCTGGGA AATTCTCCTG TTCCTGCTGC CCTTCGGAGT GGTCAGTGCT 
GTCCGGCAGT TGGAGGAAAA TCAGAGCATA AAGAATGATC ACCATCATTC GGTGGAGCGA 
CAGGTGCGAC GTCGAAATAA TAAAAGTAAT GCCTCAGTGG ATCACCATCG GCATGCGCCA 
TCTAGCGGAT CAAAGAAGAA CAGTCGGAGC CATTCTGTAA GCCTGGATTC GGATGACTAC 
GATTGGCTGG ATGCGGATAC TAGTGAGGCA ATCGATAGCT CCGAGGTCAC GGCTGTGGAA 
TCTATAAGAT CACCCGGAGA ATCATATGAT ATCTTCACAT GTTGCAATCA GGTCTTCGGC 
TCATGTCGCA CAGCATGCGA AAATTTATCT CTGGTGGAGT TTGCCACTGG CACTGGCGGC 
GACAATCGCG ATGAACTGCA CAGATATTGT CAACTGCACC AGGTGGAATT TTGGACCTGC 
GTCAACCAGA CTTTCGACGC GATCACCCGT GGAGCCGACT GGTCGGGAAG ACGCTGTTGC 
CAATTTGGAG TCCTGCCCCA TTGCCGCAAC GTCTGCGCCA CTTCCACTCG CTCACCGGTG 
CAAAATTGTC GGCGGAGCGA CGAGCAGACG CTTTACGACT GTCTGGAGCG GCAGGAGGCA 
GCAGATCAGT GCTGCGGACA GGCCCGAACT TCCGAGTGCC TGGAGGCCTG TCGAGCCGTC 
TTCGAGCCCG CCAATGACAA CCATGACCAT GTGGACATAA ATGGAGCATG TGGAGATCGC 
AACGCGGATG TGATTCAGTG CGTGAACAAC CACACGGACA TGACTCCACT GGCAAATGCC 
GAGCAATATA TACCCTGTTG TGAGTACAGC AGCAAAGAGC ATTGTCGCCA CACCTGTCGG 
AATCTTCTGC ATCAAAACGC CACTCTTATG GAACGAAGTG AGGTAATCTT CGGCCGTTTG 
GAAGCGGCAG GATGTGGGTC ACCACTACCA CAACTGCCCT TCTGGCAGTG TTTTCTTACA 
GTGACAGGTA AACTATATGT TCCGGGAGGA TCTGGAAGAG CCGGGGGATC GTTAGCCCAA 
GGACGGATAT CGGGGCAAGG GGGATCTCTG GCTGAGCCCA ATCACCTGGG AATGGATGCG 
GCTAAAAGGC AATGCTGTGA ACAAGCCAGC AGCCACAAGT GCCGACGATT GTGCAACCAA 
ATCTTCACCA GCAATTGGTG GGATGCACGG AGCAGTTTTG AGAACGAGTG TATGGATCAG 
CCTGGAGAAA GGGAACTGCG ACGGTGTATA GAAAGCGTGG ATGCACCATG TGAACTCGGA 
TGTCAGGGTC TTAGTTTCTG CTCGAACTTC AACAATCGAC CTACGGAACT CTTTCGCAGT 
TGTACACCAG AACATGATGC AGCTGCTCGC GAGGATTGGC AGCTCCTGCA GCAGCGGGGA 
ACCGTCAGAG TCCTGGGACA GGAGCTCTTC ATCAAGAACA CCAGTCGTTG TGCTCCGGAT 
AAGTGGAGGG CTTTGGTGTG TGCCCTCCAA TTGAAGCCAT GCACGCGAGT TGGTCTCTTC 
AACGGCATTT GCAGCGAAGA TTGCCACGAG CTCTTGGATG AGTGTCTGGA CTGGACGTTA 
CAGCACCAGC GACCAAAGGA TATATGTTCT CGGTTAAAAG AAAAGGATGA TGATGATTCT 
CCACTGCCTT GTATATCCCT AGAGAGCTAT CTGAAGCCAG GGGATGCCAG TCCAGAGGAG 
TTCCAAGGTA TTACTTCACC ATGCGCCCAG AAACCCTGCA ATGGCAGTGA AGTGTGCATC 
CTGCAGCGAG GAGGCAATCA AGGATACTCC TGTATACCGG GCTGCAATTT GGGACAGGAT 
TCCAAGCTGT TTGTACCTTT CGGCTCATAT GTGCGTCTTG GAAAGAGTAA TCTCCACAAG 
AAACTGGAAG TGGGCCAATT TCCGCTGGCC GAGCATATAG TTTGCTCATG CGGACTACAA 
GGTCGCCTGG AGCAATGTCA ACCGCTACCG AGCTATATGC ATGCCCACTG CACTCTTCCA 
GGAGCGAGGA GCTACCGGCA TGGCTCCTCT TTCTACTTGG AGTGCAACCT ATGCAGTTGT 
TTCGCCGGTG AGATCACTTG CACCAAACAG CAGTGTCGTC TGCCAGGATT TGTTGATTCC 
GGTTACACCA GTTTACCGTG CAATTGTCCA GCTCACTATG TTCCCGTTTG CGGCTCCAAT 
GGCAATACTT ATCCTTCGGC ATGTGTGGCC AAGTGCCATT TGCCAGAGGG AGATTACGTC 
TACGGCGCAT GCAATGCTCG GAATGCCTGC CAGGCAGCAC CTCCAAACAG TTGTCCATCC 
GGAACTCAGT GTTTAGACAG CCGAAAGGTG TGCCTGGCCA GCATGCAGCG ACCTTGTCTG 
CAATACGTGT GTGTCAATGC CACCGCATCC AACTGTTCAA CTTTTCATCA GGGCGAGGTA 
TGCGATTCCC AGGGACGGAC TTATCCCAAT GCCTGTGCCC TGCTGAAAGC GAATCCTCAG 
GGTCAAGTTG CCTATTGGTC CGCCTGTCAA TCCAGTCGCT TTAATACCTC ACCTTCACCA 
GTCTGTGGCA TCAATGGAGT GACTTACAAA AGTAGTTATG CGGCCAGAGC GGAATATGTG 
CTGGTGGATT ATGTGGGTCG ATGTCGGGAG GTGGGACTAC TGGTAAGCGA TATGGGAAGA 
CGCTGTAGGA CTGTCAAGTG TCCCGCTCCC GTATCCAAGC ATTGTCGCTT GATAGTTCCC 
CCAGGAGCAT GTTGCCCCCT GTGTGCTGGA GGAGCCTTCA GGATAATCTA CTCCAGAAAG 
CAATTCGATC GCGCCATGTA CGGACTAAGG GCACAGAGCA GCACTCTTTT GACATTGCAA 
GGAGTGCTGC AGCAGCTGGA TGGACTGGTG CAGGTTAGCG AATGTCAACT GACTGGATTT 
CTCACCATGG AAGTGGGCAT TTTTGTGGCC ATAGTACCGT CCAGCAGTAT CAAGAGACCG 
ACTCACCTTC AATTGGAGGC CTGCGCCCGG GAGGCGGAGA AGATATCCTC CCTAATCAAT 
GCCCAATCAC CCAGAATTAC AACCAATCTA GCTCTATCCT GTTTGACAGT ATCGCATCTA 
CTAGAACCCA CGCCAAATGG AGCTACATCT TATGGTCCCC ACGCCATTTG GATACTCCCC 
TTACTTTTGT TAGTTTCTAG AGTAATCATA GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648733.2
CDS	469..3684
Translation	MRLSGWEILLFLLPFGVVSAVRQLEENQSIKNDHHHSVERQVRRRNNKSNASVDHHRHAPSSGSKKNSRSHSVSLDSDDYDWLDADTSEAIDSSEVTAVESIRSPGESYDIFTCCNQVFGSCRTACENLSLVEFATGTGGDNRDELHRYCQLHQVEFWTCVNQTFDAITRGADWSGRRCCQFGVLPHCRNVCATSTRSPVQNCRRSDEQTLYDCLERQEAADQCCGQARTSECLEACRAVFEPANDNHDHVDINGACGDRNADVIQCVNNHTDMTPLANAEQYIPCCEYSSKEHCRHTCRNLLHQNATLMERSEVIFGRLEAAGCGSPLPQLPFWQCFLTVTGKLYVPGGSGRAGGSLAQGRISGQGGSLAEPNHLGMDAAKRQCCEQASSHKCRRLCNQIFTSNWWDARSSFENECMDQPGERELRRCIESVDAPCELGCQGLSFCSNFNNRPTELFRSCTPEHDAAAREDWQLLQQRGTVRVLGQELFIKNTSRCAPDKWRALVCALQLKPCTRVGLFNGICSEDCHELLDECLDWTLQHQRPKDICSRLKEKDDDDSPLPCISLESYLKPGDASPEEFQGITSPCAQKPCNGSEVCILQRGGNQGYSCIPGCNLGQDSKLFVPFGSYVRLGKSNLHKKLEVGQFPLAEHIVCSCGLQGRLEQCQPLPSYMHAHCTLPGARSYRHGSSFYLECNLCSCFAGEITCTKQQCRLPGFVDSGYTSLPCNCPAHYVPVCGSNGNTYPSACVAKCHLPEGDYVYGACNARNACQAAPPNSCPSGTQCLDSRKVCLASMQRPCLQYVCVNATASNCSTFHQGEVCDSQGRTYPNACALLKANPQGQVAYWSACQSSRFNTSPSPVCGINGVTYKSSYAARAEYVLVDYVGRCREVGLLVSDMGRRCRTVKCPAPVSKHCRLIVPPGACCPLCAGGAFRIIYSRKQFDRAMYGLRAQSSTLLTLQGVLQQLDGLVQVSECQLTGFLTMEVGIFVAIVPSSSIKRPTHLQLEACAREAEKISSLINAQSPRITTNLALSCLTVSHLLEPTPNGATSYGPHAIWILPLLLLVSRVIIA

Target ORF information:

RefSeq Version	NM_140476.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Reversion-inducing-cysteine-rich protein with kazal motifs (Reck), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140476.3

ORF Insert Sequence:

ATGCGCCTGA GTGGCTGGGA AATTCTCCTG TTCCTGCTGC CCTTCGGAGT GGTCAGTGCT 
GTCCGGCAGT TGGAGGAAAA TCAGAGCATA AAGAATGATC ACCATCATTC GGTGGAGCGA 
CAGGTGCGAC GTCGAAATAA TAAAAGTAAT GCCTCAGTGG ATCACCATCG GCATGCGCCA 
TCTAGCGGAT CAAAGAAGAA CAGTCGGAGC CATTCTGTAA GCCTGGATTC GGATGACTAC 
GATTGGCTGG ATGCGGATAC TAGTGAGGCA ATCGATAGCT CCGAGGTCAC GGCTGTGGAA 
TCTATAAGAT CACCCGGAGA ATCATATGAT ATCTTCACAT GTTGCAATCA GGTCTTCGGC 
TCATGTCGCA CAGCATGCGA AAATTTATCT CTGGTGGAGT TTGCCACTGG CACTGGCGGC 
GACAATCGCG ATGAACTGCA CAGATATTGT CAACTGCACC AGGTGGAATT TTGGACCTGC 
GTCAACCAGA CTTTCGACGC GATCACCCGT GGAGCCGACT GGTCGGGAAG ACGCTGTTGC 
CAATTTGGAG TCCTGCCCCA TTGCCGCAAC GTCTGCGCCA CTTCCACTCG CTCACCGGTG 
CAAAATTGTC GGCGGAGCGA CGAGCAGACG CTTTACGACT GTCTGGAGCG GCAGGAGGCA 
GCAGATCAGT GCTGCGGACA GGCCCGAACT TCCGAGTGCC TGGAGGCCTG TCGAGCCGTC 
TTCGAGCCCG CCAATGACAA CCATGACCAT GTGGACATAA ATGGAGCATG TGGAGATCGC 
AACGCGGATG TGATTCAGTG CGTGAACAAC CACACGGACA TGACTCCACT GGCAAATGCC 
GAGCAATATA TACCCTGTTG TGAGTACAGC AGCAAAGAGC ATTGTCGCCA CACCTGTCGG 
AATCTTCTGC ATCAAAACGC CACTCTTATG GAACGAAGTG AGGTAATCTT CGGCCGTTTG 
GAAGCGGCAG GATGTGGGTC ACCACTACCA CAACTGCCCT TCTGGCAGTG TTTTCTTACA 
GTGACAGGTA AACTATATGT TCCGGGAGGA TCTGGAAGAG CCGGGGGATC GTTAGCCCAA 
GGACGGATAT CGGGGCAAGG GGGATCTCTG GCTGAGCCCA ATCACCTGGG AATGGATGCG 
GCTAAAAGGC AATGCTGTGA ACAAGCCAGC AGCCACAAGT GCCGACGATT GTGCAACCAA 
ATCTTCACCA GCAATTGGTG GGATGCACGG AGCAGTTTTG AGAACGAGTG TATGGATCAG 
CCTGGAGAAA GGGAACTGCG ACGGTGTATA GAAAGCGTGG ATGCACCATG TGAACTCGGA 
TGTCAGGGTC TTAGTTTCTG CTCGAACTTC AACAATCGAC CTACGGAACT CTTTCGCAGT 
TGTACACCAG AACATGATGC AGCTGCTCGC GAGGATTGGC AGCTCCTGCA GCAGCGGGGA 
ACCGTCAGAG TCCTGGGACA GGAGCTCTTC ATCAAGAACA CCAGTCGTTG TGCTCCGGAT 
AAGTGGAGGG CTTTGGTGTG TGCCCTCCAA TTGAAGCCAT GCACGCGAGT TGGTCTCTTC 
AACGGCATTT GCAGCGAAGA TTGCCACGAG CTCTTGGATG AGTGTCTGGA CTGGACGTTA 
CAGCACCAGC GACCAAAGGA TATATGTTCT CGGTTAAAAG AAAAGGATGA TGATGATTCT 
CCACTGCCTT GTATATCCCT AGAGAGCTAT CTGAAGCCAG GGGATGCCAG TCCAGAGGAG 
TTCCAAGGTA TTACTTCACC ATGCGCCCAG AAACCCTGCA ATGGCAGTGA AGTGTGCATC 
CTGCAGCGAG GAGGCAATCA AGGATACTCC TGTATACCGG GCTGCAATTT GGGACAGGAT 
TCCAAGCTGT TTGTACCTTT CGGCTCATAT GTGCGTCTTG GAAAGAGTAA TCTCCACAAG 
AAACTGGAAG TGGGCCAATT TCCGCTGGCC GAGCATATAG TTTGCTCATG CGGACTACAA 
GGTCGCCTGG AGCAATGTCA ACCGCTACCG AGCTATATGC ATGCCCACTG CACTCTTCCA 
GGAGCGAGGA GCTACCGGCA TGGCTCCTCT TTCTACTTGG AGTGCAACCT ATGCAGTTGT 
TTCGCCGGTG AGATCACTTG CACCAAACAG CAGTGTCGTC TGCCAGGATT TGTTGATTCC 
GGTTACACCA GTTTACCGTG CAATTGTCCA GCTCACTATG TTCCCGTTTG CGGCTCCAAT 
GGCAATACTT ATCCTTCGGC ATGTGTGGCC AAGTGCCATT TGCCAGAGGG AGATTACGTC 
TACGGCGCAT GCAATGCTCG GAATGCCTGC CAGGCAGCAC CTCCAAACAG TTGTCCATCC 
GGAACTCAGT GTTTAGACAG CCGAAAGGTG TGCCTGGCCA GCATGCAGCG ACCTTGTCTG 
CAATACGTGT GTGTCAATGC CACCGCATCC AACTGTTCAA CTTTTCATCA GGGCGAGGTA 
TGCGATTCCC AGGGACGGAC TTATCCCAAT GCCTGTGCCC TGCTGAAAGC GAATCCTCAG 
GGTCAAGTTG CCTATTGGTC CGCCTGTCAA TCCAGTCGCT TTAATACCTC ACCTTCACCA 
GTCTGTGGCA TCAATGGAGT GACTTACAAA AGTAGTTATG CGGCCAGAGC GGAATATGTG 
CTGGTGGATT ATGTGGGTCG ATGTCGGGAG GTGGGACTAC TGGTAAGCGA TATGGGAAGA 
CGCTGTAGGA CTGTCAAGTG TCCCGCTCCC GTATCCAAGC ATTGTCGCTT GATAGTTCCC 
CCAGGAGCAT GTTGCCCCCT GTGTGCTGGA GGAGCCTTCA GGATAATCTA CTCCAGAAAG 
CAATTCGATC GCGCCATGTA CGGACTAAGG GCACAGAGCA GCACTCTTTT GACATTGCAA 
GGAGTGCTGC AGCAGCTGGA TGGACTGGTG CAGGTTAGCG AATGTCAACT GACTGGATTT 
CTCACCATGG AAGTGGGCAT TTTTGTGGCC ATAGTACCGT CCAGCAGTAT CAAGAGACCG 
ACTCACCTTC AATTGGAGGC CTGCGCCCGG GAGGCGGAGA AGATATCCTC CCTAATCAAT 
GCCCAATCAC CCAGAATTAC AACCAATCTA GCTCTATCCT GTTTGACAGT ATCGCATCTA 
CTAGAACCCA CGCCAAATGG AGCTACATCT TATGGTCCCC ACGCCATTTG GATACTCCCC 
TTACTTTTGT TAGTTTCTAG AGTAATCATA GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Reck ( NM_001274924.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261853.1 Drosophila melanogaster Reversion-inducing-cysteine-rich protein with kazal motifs (Reck), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18518
Clone ID Related Accession (Same CDS sequence)	NM_140476.3 , NM_001274924.1
Accession Version	NM_001274924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Reversion-inducing-cysteine-rich protein with kazal motifs, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCCTGA GTGGCTGGGA AATTCTCCTG TTCCTGCTGC CCTTCGGAGT GGTCAGTGCT 
GTCCGGCAGT TGGAGGAAAA TCAGAGCATA AAGAATGATC ACCATCATTC GGTGGAGCGA 
CAGGTGCGAC GTCGAAATAA TAAAAGTAAT GCCTCAGTGG ATCACCATCG GCATGCGCCA 
TCTAGCGGAT CAAAGAAGAA CAGTCGGAGC CATTCTGTAA GCCTGGATTC GGATGACTAC 
GATTGGCTGG ATGCGGATAC TAGTGAGGCA ATCGATAGCT CCGAGGTCAC GGCTGTGGAA 
TCTATAAGAT CACCCGGAGA ATCATATGAT ATCTTCACAT GTTGCAATCA GGTCTTCGGC 
TCATGTCGCA CAGCATGCGA AAATTTATCT CTGGTGGAGT TTGCCACTGG CACTGGCGGC 
GACAATCGCG ATGAACTGCA CAGATATTGT CAACTGCACC AGGTGGAATT TTGGACCTGC 
GTCAACCAGA CTTTCGACGC GATCACCCGT GGAGCCGACT GGTCGGGAAG ACGCTGTTGC 
CAATTTGGAG TCCTGCCCCA TTGCCGCAAC GTCTGCGCCA CTTCCACTCG CTCACCGGTG 
CAAAATTGTC GGCGGAGCGA CGAGCAGACG CTTTACGACT GTCTGGAGCG GCAGGAGGCA 
GCAGATCAGT GCTGCGGACA GGCCCGAACT TCCGAGTGCC TGGAGGCCTG TCGAGCCGTC 
TTCGAGCCCG CCAATGACAA CCATGACCAT GTGGACATAA ATGGAGCATG TGGAGATCGC 
AACGCGGATG TGATTCAGTG CGTGAACAAC CACACGGACA TGACTCCACT GGCAAATGCC 
GAGCAATATA TACCCTGTTG TGAGTACAGC AGCAAAGAGC ATTGTCGCCA CACCTGTCGG 
AATCTTCTGC ATCAAAACGC CACTCTTATG GAACGAAGTG AGGTAATCTT CGGCCGTTTG 
GAAGCGGCAG GATGTGGGTC ACCACTACCA CAACTGCCCT TCTGGCAGTG TTTTCTTACA 
GTGACAGGTA AACTATATGT TCCGGGAGGA TCTGGAAGAG CCGGGGGATC GTTAGCCCAA 
GGACGGATAT CGGGGCAAGG GGGATCTCTG GCTGAGCCCA ATCACCTGGG AATGGATGCG 
GCTAAAAGGC AATGCTGTGA ACAAGCCAGC AGCCACAAGT GCCGACGATT GTGCAACCAA 
ATCTTCACCA GCAATTGGTG GGATGCACGG AGCAGTTTTG AGAACGAGTG TATGGATCAG 
CCTGGAGAAA GGGAACTGCG ACGGTGTATA GAAAGCGTGG ATGCACCATG TGAACTCGGA 
TGTCAGGGTC TTAGTTTCTG CTCGAACTTC AACAATCGAC CTACGGAACT CTTTCGCAGT 
TGTACACCAG AACATGATGC AGCTGCTCGC GAGGATTGGC AGCTCCTGCA GCAGCGGGGA 
ACCGTCAGAG TCCTGGGACA GGAGCTCTTC ATCAAGAACA CCAGTCGTTG TGCTCCGGAT 
AAGTGGAGGG CTTTGGTGTG TGCCCTCCAA TTGAAGCCAT GCACGCGAGT TGGTCTCTTC 
AACGGCATTT GCAGCGAAGA TTGCCACGAG CTCTTGGATG AGTGTCTGGA CTGGACGTTA 
CAGCACCAGC GACCAAAGGA TATATGTTCT CGGTTAAAAG AAAAGGATGA TGATGATTCT 
CCACTGCCTT GTATATCCCT AGAGAGCTAT CTGAAGCCAG GGGATGCCAG TCCAGAGGAG 
TTCCAAGGTA TTACTTCACC ATGCGCCCAG AAACCCTGCA ATGGCAGTGA AGTGTGCATC 
CTGCAGCGAG GAGGCAATCA AGGATACTCC TGTATACCGG GCTGCAATTT GGGACAGGAT 
TCCAAGCTGT TTGTACCTTT CGGCTCATAT GTGCGTCTTG GAAAGAGTAA TCTCCACAAG 
AAACTGGAAG TGGGCCAATT TCCGCTGGCC GAGCATATAG TTTGCTCATG CGGACTACAA 
GGTCGCCTGG AGCAATGTCA ACCGCTACCG AGCTATATGC ATGCCCACTG CACTCTTCCA 
GGAGCGAGGA GCTACCGGCA TGGCTCCTCT TTCTACTTGG AGTGCAACCT ATGCAGTTGT 
TTCGCCGGTG AGATCACTTG CACCAAACAG CAGTGTCGTC TGCCAGGATT TGTTGATTCC 
GGTTACACCA GTTTACCGTG CAATTGTCCA GCTCACTATG TTCCCGTTTG CGGCTCCAAT 
GGCAATACTT ATCCTTCGGC ATGTGTGGCC AAGTGCCATT TGCCAGAGGG AGATTACGTC 
TACGGCGCAT GCAATGCTCG GAATGCCTGC CAGGCAGCAC CTCCAAACAG TTGTCCATCC 
GGAACTCAGT GTTTAGACAG CCGAAAGGTG TGCCTGGCCA GCATGCAGCG ACCTTGTCTG 
CAATACGTGT GTGTCAATGC CACCGCATCC AACTGTTCAA CTTTTCATCA GGGCGAGGTA 
TGCGATTCCC AGGGACGGAC TTATCCCAAT GCCTGTGCCC TGCTGAAAGC GAATCCTCAG 
GGTCAAGTTG CCTATTGGTC CGCCTGTCAA TCCAGTCGCT TTAATACCTC ACCTTCACCA 
GTCTGTGGCA TCAATGGAGT GACTTACAAA AGTAGTTATG CGGCCAGAGC GGAATATGTG 
CTGGTGGATT ATGTGGGTCG ATGTCGGGAG GTGGGACTAC TGGTAAGCGA TATGGGAAGA 
CGCTGTAGGA CTGTCAAGTG TCCCGCTCCC GTATCCAAGC ATTGTCGCTT GATAGTTCCC 
CCAGGAGCAT GTTGCCCCCT GTGTGCTGGA GGAGCCTTCA GGATAATCTA CTCCAGAAAG 
CAATTCGATC GCGCCATGTA CGGACTAAGG GCACAGAGCA GCACTCTTTT GACATTGCAA 
GGAGTGCTGC AGCAGCTGGA TGGACTGGTG CAGGTTAGCG AATGTCAACT GACTGGATTT 
CTCACCATGG AAGTGGGCAT TTTTGTGGCC ATAGTACCGT CCAGCAGTAT CAAGAGACCG 
ACTCACCTTC AATTGGAGGC CTGCGCCCGG GAGGCGGAGA AGATATCCTC CCTAATCAAT 
GCCCAATCAC CCAGAATTAC AACCAATCTA GCTCTATCCT GTTTGACAGT ATCGCATCTA 
CTAGAACCCA CGCCAAATGG AGCTACATCT TATGGTCCCC ACGCCATTTG GATACTCCCC 
TTACTTTTGT TAGTTTCTAG AGTAATCATA GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261853.1
CDS	437..3652
Translation	MRLSGWEILLFLLPFGVVSAVRQLEENQSIKNDHHHSVERQVRRRNNKSNASVDHHRHAPSSGSKKNSRSHSVSLDSDDYDWLDADTSEAIDSSEVTAVESIRSPGESYDIFTCCNQVFGSCRTACENLSLVEFATGTGGDNRDELHRYCQLHQVEFWTCVNQTFDAITRGADWSGRRCCQFGVLPHCRNVCATSTRSPVQNCRRSDEQTLYDCLERQEAADQCCGQARTSECLEACRAVFEPANDNHDHVDINGACGDRNADVIQCVNNHTDMTPLANAEQYIPCCEYSSKEHCRHTCRNLLHQNATLMERSEVIFGRLEAAGCGSPLPQLPFWQCFLTVTGKLYVPGGSGRAGGSLAQGRISGQGGSLAEPNHLGMDAAKRQCCEQASSHKCRRLCNQIFTSNWWDARSSFENECMDQPGERELRRCIESVDAPCELGCQGLSFCSNFNNRPTELFRSCTPEHDAAAREDWQLLQQRGTVRVLGQELFIKNTSRCAPDKWRALVCALQLKPCTRVGLFNGICSEDCHELLDECLDWTLQHQRPKDICSRLKEKDDDDSPLPCISLESYLKPGDASPEEFQGITSPCAQKPCNGSEVCILQRGGNQGYSCIPGCNLGQDSKLFVPFGSYVRLGKSNLHKKLEVGQFPLAEHIVCSCGLQGRLEQCQPLPSYMHAHCTLPGARSYRHGSSFYLECNLCSCFAGEITCTKQQCRLPGFVDSGYTSLPCNCPAHYVPVCGSNGNTYPSACVAKCHLPEGDYVYGACNARNACQAAPPNSCPSGTQCLDSRKVCLASMQRPCLQYVCVNATASNCSTFHQGEVCDSQGRTYPNACALLKANPQGQVAYWSACQSSRFNTSPSPVCGINGVTYKSSYAARAEYVLVDYVGRCREVGLLVSDMGRRCRTVKCPAPVSKHCRLIVPPGACCPLCAGGAFRIIYSRKQFDRAMYGLRAQSSTLLTLQGVLQQLDGLVQVSECQLTGFLTMEVGIFVAIVPSSSIKRPTHLQLEACAREAEKISSLINAQSPRITTNLALSCLTVSHLLEPTPNGATSYGPHAIWILPLLLLVSRVIIA

Target ORF information:

RefSeq Version	NM_001274924.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Reversion-inducing-cysteine-rich protein with kazal motifs (Reck), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274924.1

ORF Insert Sequence:

ATGCGCCTGA GTGGCTGGGA AATTCTCCTG TTCCTGCTGC CCTTCGGAGT GGTCAGTGCT 
GTCCGGCAGT TGGAGGAAAA TCAGAGCATA AAGAATGATC ACCATCATTC GGTGGAGCGA 
CAGGTGCGAC GTCGAAATAA TAAAAGTAAT GCCTCAGTGG ATCACCATCG GCATGCGCCA 
TCTAGCGGAT CAAAGAAGAA CAGTCGGAGC CATTCTGTAA GCCTGGATTC GGATGACTAC 
GATTGGCTGG ATGCGGATAC TAGTGAGGCA ATCGATAGCT CCGAGGTCAC GGCTGTGGAA 
TCTATAAGAT CACCCGGAGA ATCATATGAT ATCTTCACAT GTTGCAATCA GGTCTTCGGC 
TCATGTCGCA CAGCATGCGA AAATTTATCT CTGGTGGAGT TTGCCACTGG CACTGGCGGC 
GACAATCGCG ATGAACTGCA CAGATATTGT CAACTGCACC AGGTGGAATT TTGGACCTGC 
GTCAACCAGA CTTTCGACGC GATCACCCGT GGAGCCGACT GGTCGGGAAG ACGCTGTTGC 
CAATTTGGAG TCCTGCCCCA TTGCCGCAAC GTCTGCGCCA CTTCCACTCG CTCACCGGTG 
CAAAATTGTC GGCGGAGCGA CGAGCAGACG CTTTACGACT GTCTGGAGCG GCAGGAGGCA 
GCAGATCAGT GCTGCGGACA GGCCCGAACT TCCGAGTGCC TGGAGGCCTG TCGAGCCGTC 
TTCGAGCCCG CCAATGACAA CCATGACCAT GTGGACATAA ATGGAGCATG TGGAGATCGC 
AACGCGGATG TGATTCAGTG CGTGAACAAC CACACGGACA TGACTCCACT GGCAAATGCC 
GAGCAATATA TACCCTGTTG TGAGTACAGC AGCAAAGAGC ATTGTCGCCA CACCTGTCGG 
AATCTTCTGC ATCAAAACGC CACTCTTATG GAACGAAGTG AGGTAATCTT CGGCCGTTTG 
GAAGCGGCAG GATGTGGGTC ACCACTACCA CAACTGCCCT TCTGGCAGTG TTTTCTTACA 
GTGACAGGTA AACTATATGT TCCGGGAGGA TCTGGAAGAG CCGGGGGATC GTTAGCCCAA 
GGACGGATAT CGGGGCAAGG GGGATCTCTG GCTGAGCCCA ATCACCTGGG AATGGATGCG 
GCTAAAAGGC AATGCTGTGA ACAAGCCAGC AGCCACAAGT GCCGACGATT GTGCAACCAA 
ATCTTCACCA GCAATTGGTG GGATGCACGG AGCAGTTTTG AGAACGAGTG TATGGATCAG 
CCTGGAGAAA GGGAACTGCG ACGGTGTATA GAAAGCGTGG ATGCACCATG TGAACTCGGA 
TGTCAGGGTC TTAGTTTCTG CTCGAACTTC AACAATCGAC CTACGGAACT CTTTCGCAGT 
TGTACACCAG AACATGATGC AGCTGCTCGC GAGGATTGGC AGCTCCTGCA GCAGCGGGGA 
ACCGTCAGAG TCCTGGGACA GGAGCTCTTC ATCAAGAACA CCAGTCGTTG TGCTCCGGAT 
AAGTGGAGGG CTTTGGTGTG TGCCCTCCAA TTGAAGCCAT GCACGCGAGT TGGTCTCTTC 
AACGGCATTT GCAGCGAAGA TTGCCACGAG CTCTTGGATG AGTGTCTGGA CTGGACGTTA 
CAGCACCAGC GACCAAAGGA TATATGTTCT CGGTTAAAAG AAAAGGATGA TGATGATTCT 
CCACTGCCTT GTATATCCCT AGAGAGCTAT CTGAAGCCAG GGGATGCCAG TCCAGAGGAG 
TTCCAAGGTA TTACTTCACC ATGCGCCCAG AAACCCTGCA ATGGCAGTGA AGTGTGCATC 
CTGCAGCGAG GAGGCAATCA AGGATACTCC TGTATACCGG GCTGCAATTT GGGACAGGAT 
TCCAAGCTGT TTGTACCTTT CGGCTCATAT GTGCGTCTTG GAAAGAGTAA TCTCCACAAG 
AAACTGGAAG TGGGCCAATT TCCGCTGGCC GAGCATATAG TTTGCTCATG CGGACTACAA 
GGTCGCCTGG AGCAATGTCA ACCGCTACCG AGCTATATGC ATGCCCACTG CACTCTTCCA 
GGAGCGAGGA GCTACCGGCA TGGCTCCTCT TTCTACTTGG AGTGCAACCT ATGCAGTTGT 
TTCGCCGGTG AGATCACTTG CACCAAACAG CAGTGTCGTC TGCCAGGATT TGTTGATTCC 
GGTTACACCA GTTTACCGTG CAATTGTCCA GCTCACTATG TTCCCGTTTG CGGCTCCAAT 
GGCAATACTT ATCCTTCGGC ATGTGTGGCC AAGTGCCATT TGCCAGAGGG AGATTACGTC 
TACGGCGCAT GCAATGCTCG GAATGCCTGC CAGGCAGCAC CTCCAAACAG TTGTCCATCC 
GGAACTCAGT GTTTAGACAG CCGAAAGGTG TGCCTGGCCA GCATGCAGCG ACCTTGTCTG 
CAATACGTGT GTGTCAATGC CACCGCATCC AACTGTTCAA CTTTTCATCA GGGCGAGGTA 
TGCGATTCCC AGGGACGGAC TTATCCCAAT GCCTGTGCCC TGCTGAAAGC GAATCCTCAG 
GGTCAAGTTG CCTATTGGTC CGCCTGTCAA TCCAGTCGCT TTAATACCTC ACCTTCACCA 
GTCTGTGGCA TCAATGGAGT GACTTACAAA AGTAGTTATG CGGCCAGAGC GGAATATGTG 
CTGGTGGATT ATGTGGGTCG ATGTCGGGAG GTGGGACTAC TGGTAAGCGA TATGGGAAGA 
CGCTGTAGGA CTGTCAAGTG TCCCGCTCCC GTATCCAAGC ATTGTCGCTT GATAGTTCCC 
CCAGGAGCAT GTTGCCCCCT GTGTGCTGGA GGAGCCTTCA GGATAATCTA CTCCAGAAAG 
CAATTCGATC GCGCCATGTA CGGACTAAGG GCACAGAGCA GCACTCTTTT GACATTGCAA 
GGAGTGCTGC AGCAGCTGGA TGGACTGGTG CAGGTTAGCG AATGTCAACT GACTGGATTT 
CTCACCATGG AAGTGGGCAT TTTTGTGGCC ATAGTACCGT CCAGCAGTAT CAAGAGACCG 
ACTCACCTTC AATTGGAGGC CTGCGCCCGG GAGGCGGAGA AGATATCCTC CCTAATCAAT 
GCCCAATCAC CCAGAATTAC AACCAATCTA GCTCTATCCT GTTTGACAGT ATCGCATCTA 
CTAGAACCCA CGCCAAATGG AGCTACATCT TATGGTCCCC ACGCCATTTG GATACTCCCC 
TTACTTTTGT TAGTTTCTAG AGTAATCATA GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Reck cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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