stwl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	stwl
Entrez Gene ID	39581
Full Name	stonewall
Synonyms	84,CG3836,Dmel\CG3836,Stwl,fs(3)02024,snw
General protein information	Preferred Names stonewall Names CG3836 gene product from transcript CG3836-RC CG3836-PA CG3836-PB CG3836-PC STONEWALL alpha transcript stwl-PA stwl-PB stwl-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 70D6-70D7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274910.1	NP_001261839.1	stonewall, isoform C
NM_001274909.1	NP_001261838.1	stonewall, isoform B
NM_079344.3	NP_524068.2	stonewall, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The identification of stonewall (stwl) as a novel hydroxyurea-hypersensitive mutant, is reported.
Title: Stwl modifies chromatin compaction and is required to maintain DNA integrity in the presence of perturbed DNA replication.

Stwl-dependent transcriptional repression does not target bam, but rather Stwl represses the expression of many genes, including those that may be targeted by Nanos and Pumilio translational inhibition.
Title: Stonewalling Drosophila stem cell differentiation by epigenetic controls.

stwl(UY823) overexpression, like overexpression of the wild-type gene, activated G1/S transition and apoptosis
Title: The myb-related gene stonewall induces both hyperplasia and cell death in Drosophila: rescue of fly lethality by coexpression of apoptosis inducers.

The following stwl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the stwl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa20948	NM_001274909.1 Latest version!	Drosophila melanogaster stonewall (stwl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa18496	NM_079344.3 Latest version!	Drosophila melanogaster stonewall (stwl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18496	NM_001274910.1 Latest version!	Drosophila melanogaster stonewall (stwl), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

stwl ( NM_001274909.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261838.1 Drosophila melanogaster stonewall (stwl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa20948
Clone ID Related Accession (Same CDS sequence)	NM_001274909.1
Accession Version	NM_001274909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stonewall, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGGGGG ACTTCTTATT CGAGAATTCT TGCTTGGAAA AATGCAAGCG ACGCTGGCGT 
CAGCTGCGAA ATGACTATAC CCGCTGGTGC AACGCCGACG CCAACCGGCG ACGAAATGGC 
CAGCGTCGTT TGGCTTATCC GCTGGCCGAT GAGCTGCGCT TTTTGGACCG CCATCTAAAC 
ATAGCTGACG ACATGGCTGC CGACGATGAC CGCAGCGTAT CATCGGACAA GGACAGGGAT 
AACAATAGAG ACAGCGAGGG CGTGGATCAT CATGCTCAGG CGTCATTAAA AGAACGCGCA 
TCATCGACAA GCAAGTTAGT TAAGGAGGTC AAGCTGGCAA GTCAAGTTCG TAAGGAAAAA 
TCGTCCCAAG ACAAGAGAGA AAATTGGGAA AATCCTGGAG ATAAACAGCG ATCTAGGAAG 
AAAAGTGCTG AAGAGAAGCT GAATGATTTG GAGGAATCGG ACGAACCAGA GAAGGTTCCT 
GAACTCGATA GTTTTCTGCA GTCTGATAAT GAAGACGACG AGTGCATGGA CGAGGAGCAC 
CTAGAAGATC TGGAAGGTTT CGATTTTGAC TTGGAACAAT CGAATCAGGA AAAGGAGTTA 
ACGCCGGAGA AGTCTCTAGA GAAGAATAAC ACCGACTCAA CTGTTAGCCA GCCGGAGTTC 
TTTGTCAAGC AAACCAGAGA TCCTCGTTTG CTAATTATTG GCAGAAAGAA TTCGACGTCT 
TCGTTTGCTG AATCCAAGGC TTCAAAATCT ATTTCCGGGG ATCCTCTGGA AACGGCTATG 
GAAGATAGTG GCGATGAATA TGGTAGGGAT GGTGAAAAAC GCGTCACAGG CAGCGACACT 
ACTTCTCCAA CCCGACGTCC ACGTCGCGCA GCCCGTGCAT CAATAAGTTT CGCTGGCATA 
AGGGATTTAC CGATCCAGAA ACCAGGCCAA GCACAACGAA TGACGCGATT GCAGCGCAGG 
AAATCTATGA GTTTAGCGGC TGTACACAGT GTAAGGAGCT CCACATCGCC TGTGAAAATG 
ACTCCAGTCC CCAGGTCTCA GCCGATCAAC AAGCCGCCAT CCGGGCCAGT CCAGATCACG 
AAAATCAGTC ATCGGGATGA CATCTTCCCG AGACCTGCGG TGCCATCTGG AGTTGTGAAT 
ATAAATCAAA ACCAACTCAA GACTCAACAA CAGCAGAAGA CGCTGATCTT TCCGACGACC 
GATGAAGCGA TCGCTAACCC GCAGGGATCT GGTCCACCAC AAAGGAGGTC CGTGGGTAGG 
CCGCCCAAGA AGCTACCTAT GGTGCAGCGA ATAGTTGCGC CGGAAACGCA GACCAAGCCA 
CAGTCGCCCA TAAACACCAA AGTCCTGACC ATTGGCAAGC CCACCAGCTC CGTCGCGAGT 
GGTAACATAA ATAATATTGC TATCAAAAGC AACGTCGTTA CTAGCATAAG CAGCAGCACC 
GCTACTACTA ATACCATTAC CAAAGGTACC AGTAACAACA GCATCAAAAC TACTGAAAAC 
GTTAATGTAA TAAGCTACAG TCCGAATTCA TCTAGCACCA GCAGCAGCAG CAAGGCCACC 
ACTGGTGCCT CTGGTGCGGT GATAACTAAT ATACCCACAA CCACCACAGC TGGAACATCC 
GTTCCGGTCG GAAAGAGTAT AACCCAATTG AAAATGACTG AGCGAGGCAC TCAAACCGGA 
GTCCAGAATC CTTTCTCCGA TAATTACTTT CTCGAAATGA TCAAGCCCCA GATGCAGGAA 
ATGAACCCCC GTCAGAAGAT GCACTTTAAG AAGAAAGTAT TCCAAGCGCT GATGGAAACC 
TTTGATGATG CTACCGATTT CCCAACGTCT AAAGAACTGC AGCATTTTAA TATAAACACT 
CCTTCAGGAT TTGAGCACAT TACAGATCCG GAACTTCGAT TGGTCAGGGA GCTAGTCAGC 
ATGGTGAGCG CGGCTAAGGT TACCCTAATT AGACCGCCAG GAGAAGCTAC AGCTATAGCT 
ACAGCTTCCC GCAGTGGAAT TGCTCCTGAG GTACAGCGAG GACCCCGAAC AAATATGACA 
CGCCAAGTCA TTCAGAGGGT ATACAAGCCA GGCACTGGAC AAGAGATCGC CCCCACCAGC 
CCGGCGGGTG GACTAGATAA GAGGTTGTTC AGGTTAGCGG CTATGGGTGG TAAACCGAAT 
GGCAACTTGA GTGCCACTCA GGAAATTTTG CGCAAGGATA GTGTTGACAG TAACCATAGT 
GTGGTCAAGG TGGCCAACCA CGCCCCAACA GGCAGTCCCA AAGGAGCTGC TGTAGTGGCT 
GCTATAAGGC CGCAGGGTTC ATTGATCAAC AGTTTCTTTG GACAAGGCAA TGGACCGACG 
ACGGCCAAGG GAGCTGCCTC CGAGAATATC CGCGCCATGT CCAGGAGGTA TTCCGTGTGC 
GGTAGCAGCA ATCCACCCAA TGCCCAAAAC GCTAGCCAAG GTTCCGCCAA TGGCAGCACC 
ATAAACTCCA ATGCTAGCGC TATGGAAGCG TCTATGCTTA AGCGGCGATT GATAGCAGCT 
GGGCATGGAA TGGTGCCACC TACCCAACGC CCTCGGTATT CTGCCGTAGG TGCTAGTCAA 
ATGACCACTG CATCGCAAGG CAGCAGTTTA CTAGTGAGGA AGTCTGTGGG CTGCGTTCCT 
GCTTACCAAA AGCAAATCTC ACCCACTGGC GGTGCCTCCC TGCTTCAGAA AACTCCGCAG 
ATTGCCAGTG TGCAAGGATC TGCCTTCAAT GACTTTGCCC AGCCCAAGCC CGCTGCAACG 
GCCTCTGTAA ACGGCGAAGA AAGTTCGCCT ACCTCGGCGC TGAAGCGCAG CTTGGTGGTG 
GCTAATACCA AAACCTCCTT TCAGGACCTT CTTCAGCAGG CCAGTCAGAC GGTGCAGAGA 
AATAAAATTG AATCCTCGGA GACAGCAGCC ACTATTGCTG CGGATGATTT TTCGCTGGAC 
AACCTTAAGC GAGAGCCAGT GGATCCCGCG GAGGATCACA ATGACATACT CGGTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261838.1
CDS	68..3067
Translation	MMGDFLFENSCLEKCKRRWRQLRNDYTRWCNADANRRRNGQRRLAYPLADELRFLDRHLNIADDMAADDDRSVSSDKDRDNNRDSEGVDHHAQASLKERASSTSKLVKEVKLASQVRKEKSSQDKRENWENPGDKQRSRKKSAEEKLNDLEESDEPEKVPELDSFLQSDNEDDECMDEEHLEDLEGFDFDLEQSNQEKELTPEKSLEKNNTDSTVSQPEFFVKQTRDPRLLIIGRKNSTSSFAESKASKSISGDPLETAMEDSGDEYGRDGEKRVTGSDTTSPTRRPRRAARASISFAGIRDLPIQKPGQAQRMTRLQRRKSMSLAAVHSVRSSTSPVKMTPVPRSQPINKPPSGPVQITKISHRDDIFPRPAVPSGVVNINQNQLKTQQQQKTLIFPTTDEAIANPQGSGPPQRRSVGRPPKKLPMVQRIVAPETQTKPQSPINTKVLTIGKPTSSVASGNINNIAIKSNVVTSISSSTATTNTITKGTSNNSIKTTENVNVISYSPNSSSTSSSSKATTGASGAVITNIPTTTTAGTSVPVGKSITQLKMTERGTQTGVQNPFSDNYFLEMIKPQMQEMNPRQKMHFKKKVFQALMETFDDATDFPTSKELQHFNINTPSGFEHITDPELRLVRELVSMVSAAKVTLIRPPGEATAIATASRSGIAPEVQRGPRTNMTRQVIQRVYKPGTGQEIAPTSPAGGLDKRLFRLAAMGGKPNGNLSATQEILRKDSVDSNHSVVKVANHAPTGSPKGAAVVAAIRPQGSLINSFFGQGNGPTTAKGAASENIRAMSRRYSVCGSSNPPNAQNASQGSANGSTINSNASAMEASMLKRRLIAAGHGMVPPTQRPRYSAVGASQMTTASQGSSLLVRKSVGCVPAYQKQISPTGGASLLQKTPQIASVQGSAFNDFAQPKPAATASVNGEESSPTSALKRSLVVANTKTSFQDLLQQASQTVQRNKIESSETAATIAADDFSLDNLKREPVDPAEDHNDILGV

Target ORF information:

RefSeq Version	NM_001274909.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stonewall (stwl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274909.1

ORF Insert Sequence:

ATGATGGGGG ACTTCTTATT CGAGAATTCT TGCTTGGAAA AATGCAAGCG ACGCTGGCGT 
CAGCTGCGAA ATGACTATAC CCGCTGGTGC AACGCCGACG CCAACCGGCG ACGAAATGGC 
CAGCGTCGTT TGGCTTATCC GCTGGCCGAT GAGCTGCGCT TTTTGGACCG CCATCTAAAC 
ATAGCTGACG ACATGGCTGC CGACGATGAC CGCAGCGTAT CATCGGACAA GGACAGGGAT 
AACAATAGAG ACAGCGAGGG CGTGGATCAT CATGCTCAGG CGTCATTAAA AGAACGCGCA 
TCATCGACAA GCAAGTTAGT TAAGGAGGTC AAGCTGGCAA GTCAAGTTCG TAAGGAAAAA 
TCGTCCCAAG ACAAGAGAGA AAATTGGGAA AATCCTGGAG ATAAACAGCG ATCTAGGAAG 
AAAAGTGCTG AAGAGAAGCT GAATGATTTG GAGGAATCGG ACGAACCAGA GAAGGTTCCT 
GAACTCGATA GTTTTCTGCA GTCTGATAAT GAAGACGACG AGTGCATGGA CGAGGAGCAC 
CTAGAAGATC TGGAAGGTTT CGATTTTGAC TTGGAACAAT CGAATCAGGA AAAGGAGTTA 
ACGCCGGAGA AGTCTCTAGA GAAGAATAAC ACCGACTCAA CTGTTAGCCA GCCGGAGTTC 
TTTGTCAAGC AAACCAGAGA TCCTCGTTTG CTAATTATTG GCAGAAAGAA TTCGACGTCT 
TCGTTTGCTG AATCCAAGGC TTCAAAATCT ATTTCCGGGG ATCCTCTGGA AACGGCTATG 
GAAGATAGTG GCGATGAATA TGGTAGGGAT GGTGAAAAAC GCGTCACAGG CAGCGACACT 
ACTTCTCCAA CCCGACGTCC ACGTCGCGCA GCCCGTGCAT CAATAAGTTT CGCTGGCATA 
AGGGATTTAC CGATCCAGAA ACCAGGCCAA GCACAACGAA TGACGCGATT GCAGCGCAGG 
AAATCTATGA GTTTAGCGGC TGTACACAGT GTAAGGAGCT CCACATCGCC TGTGAAAATG 
ACTCCAGTCC CCAGGTCTCA GCCGATCAAC AAGCCGCCAT CCGGGCCAGT CCAGATCACG 
AAAATCAGTC ATCGGGATGA CATCTTCCCG AGACCTGCGG TGCCATCTGG AGTTGTGAAT 
ATAAATCAAA ACCAACTCAA GACTCAACAA CAGCAGAAGA CGCTGATCTT TCCGACGACC 
GATGAAGCGA TCGCTAACCC GCAGGGATCT GGTCCACCAC AAAGGAGGTC CGTGGGTAGG 
CCGCCCAAGA AGCTACCTAT GGTGCAGCGA ATAGTTGCGC CGGAAACGCA GACCAAGCCA 
CAGTCGCCCA TAAACACCAA AGTCCTGACC ATTGGCAAGC CCACCAGCTC CGTCGCGAGT 
GGTAACATAA ATAATATTGC TATCAAAAGC AACGTCGTTA CTAGCATAAG CAGCAGCACC 
GCTACTACTA ATACCATTAC CAAAGGTACC AGTAACAACA GCATCAAAAC TACTGAAAAC 
GTTAATGTAA TAAGCTACAG TCCGAATTCA TCTAGCACCA GCAGCAGCAG CAAGGCCACC 
ACTGGTGCCT CTGGTGCGGT GATAACTAAT ATACCCACAA CCACCACAGC TGGAACATCC 
GTTCCGGTCG GAAAGAGTAT AACCCAATTG AAAATGACTG AGCGAGGCAC TCAAACCGGA 
GTCCAGAATC CTTTCTCCGA TAATTACTTT CTCGAAATGA TCAAGCCCCA GATGCAGGAA 
ATGAACCCCC GTCAGAAGAT GCACTTTAAG AAGAAAGTAT TCCAAGCGCT GATGGAAACC 
TTTGATGATG CTACCGATTT CCCAACGTCT AAAGAACTGC AGCATTTTAA TATAAACACT 
CCTTCAGGAT TTGAGCACAT TACAGATCCG GAACTTCGAT TGGTCAGGGA GCTAGTCAGC 
ATGGTGAGCG CGGCTAAGGT TACCCTAATT AGACCGCCAG GAGAAGCTAC AGCTATAGCT 
ACAGCTTCCC GCAGTGGAAT TGCTCCTGAG GTACAGCGAG GACCCCGAAC AAATATGACA 
CGCCAAGTCA TTCAGAGGGT ATACAAGCCA GGCACTGGAC AAGAGATCGC CCCCACCAGC 
CCGGCGGGTG GACTAGATAA GAGGTTGTTC AGGTTAGCGG CTATGGGTGG TAAACCGAAT 
GGCAACTTGA GTGCCACTCA GGAAATTTTG CGCAAGGATA GTGTTGACAG TAACCATAGT 
GTGGTCAAGG TGGCCAACCA CGCCCCAACA GGCAGTCCCA AAGGAGCTGC TGTAGTGGCT 
GCTATAAGGC CGCAGGGTTC ATTGATCAAC AGTTTCTTTG GACAAGGCAA TGGACCGACG 
ACGGCCAAGG GAGCTGCCTC CGAGAATATC CGCGCCATGT CCAGGAGGTA TTCCGTGTGC 
GGTAGCAGCA ATCCACCCAA TGCCCAAAAC GCTAGCCAAG GTTCCGCCAA TGGCAGCACC 
ATAAACTCCA ATGCTAGCGC TATGGAAGCG TCTATGCTTA AGCGGCGATT GATAGCAGCT 
GGGCATGGAA TGGTGCCACC TACCCAACGC CCTCGGTATT CTGCCGTAGG TGCTAGTCAA 
ATGACCACTG CATCGCAAGG CAGCAGTTTA CTAGTGAGGA AGTCTGTGGG CTGCGTTCCT 
GCTTACCAAA AGCAAATCTC ACCCACTGGC GGTGCCTCCC TGCTTCAGAA AACTCCGCAG 
ATTGCCAGTG TGCAAGGATC TGCCTTCAAT GACTTTGCCC AGCCCAAGCC CGCTGCAACG 
GCCTCTGTAA ACGGCGAAGA AAGTTCGCCT ACCTCGGCGC TGAAGCGCAG CTTGGTGGTG 
GCTAATACCA AAACCTCCTT TCAGGACCTT CTTCAGCAGG CCAGTCAGAC GGTGCAGAGA 
AATAAAATTG AATCCTCGGA GACAGCAGCC ACTATTGCTG CGGATGATTT TTCGCTGGAC 
AACCTTAAGC GAGAGCCAGT GGATCCCGCG GAGGATCACA ATGACATACT CGGTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stwl ( NM_001274910.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261839.1 Drosophila melanogaster stonewall (stwl), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa18496
Clone ID Related Accession (Same CDS sequence)	NM_001274910.1 , NM_079344.3
Accession Version	NM_001274910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stonewall, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGCCG CCTCTGAGGT GAACCTGATG TTGCTGCGGT CAGTGGAGAA GCAGACTGCG 
CTTTATGATC GTACCGACGA AAACTATAGG AAGCGCCTGC CGAGTGAGAA CGCCTGGGAC 
ATGGTTGCCT CCGAAGTTGG AGAGTCGGTG GAAAAATGCA AGCGACGCTG GCGTCAGCTG 
CGAAATGACT ATACCCGCTG GTGCAACGCC GACGCCAACC GGCGACGAAA TGGCCAGCGT 
CGTTTGGCTT ATCCGCTGGC CGATGAGCTG CGCTTTTTGG ACCGCCATCT AAACATAGCT 
GACGACATGG CTGCCGACGA TGACCGCAGC GTATCATCGG ACAAGGACAG GGATAACAAT 
AGAGACAGCG AGGGCGTGGA TCATCATGCT CAGGCGTCAT TAAAAGAACG CGCATCATCG 
ACAAGCAAGT TAGTTAAGGA GGTCAAGCTG GCAAGTCAAG TTCGTAAGGA AAAATCGTCC 
CAAGACAAGA GAGAAAATTG GGAAAATCCT GGAGATAAAC AGCGATCTAG GAAGAAAAGT 
GCTGAAGAGA AGCTGAATGA TTTGGAGGAA TCGGACGAAC CAGAGAAGGT TCCTGAACTC 
GATAGTTTTC TGCAGTCTGA TAATGAAGAC GACGAGTGCA TGGACGAGGA GCACCTAGAA 
GATCTGGAAG GTTTCGATTT TGACTTGGAA CAATCGAATC AGGAAAAGGA GTTAACGCCG 
GAGAAGTCTC TAGAGAAGAA TAACACCGAC TCAACTGTTA GCCAGCCGGA GTTCTTTGTC 
AAGCAAACCA GAGATCCTCG TTTGCTAATT ATTGGCAGAA AGAATTCGAC GTCTTCGTTT 
GCTGAATCCA AGGCTTCAAA ATCTATTTCC GGGGATCCTC TGGAAACGGC TATGGAAGAT 
AGTGGCGATG AATATGGTAG GGATGGTGAA AAACGCGTCA CAGGCAGCGA CACTACTTCT 
CCAACCCGAC GTCCACGTCG CGCAGCCCGT GCATCAATAA GTTTCGCTGG CATAAGGGAT 
TTACCGATCC AGAAACCAGG CCAAGCACAA CGAATGACGC GATTGCAGCG CAGGAAATCT 
ATGAGTTTAG CGGCTGTACA CAGTGTAAGG AGCTCCACAT CGCCTGTGAA AATGACTCCA 
GTCCCCAGGT CTCAGCCGAT CAACAAGCCG CCATCCGGGC CAGTCCAGAT CACGAAAATC 
AGTCATCGGG ATGACATCTT CCCGAGACCT GCGGTGCCAT CTGGAGTTGT GAATATAAAT 
CAAAACCAAC TCAAGACTCA ACAACAGCAG AAGACGCTGA TCTTTCCGAC GACCGATGAA 
GCGATCGCTA ACCCGCAGGG ATCTGGTCCA CCACAAAGGA GGTCCGTGGG TAGGCCGCCC 
AAGAAGCTAC CTATGGTGCA GCGAATAGTT GCGCCGGAAA CGCAGACCAA GCCACAGTCG 
CCCATAAACA CCAAAGTCCT GACCATTGGC AAGCCCACCA GCTCCGTCGC GAGTGGTAAC 
ATAAATAATA TTGCTATCAA AAGCAACGTC GTTACTAGCA TAAGCAGCAG CACCGCTACT 
ACTAATACCA TTACCAAAGG TACCAGTAAC AACAGCATCA AAACTACTGA AAACGTTAAT 
GTAATAAGCT ACAGTCCGAA TTCATCTAGC ACCAGCAGCA GCAGCAAGGC CACCACTGGT 
GCCTCTGGTG CGGTGATAAC TAATATACCC ACAACCACCA CAGCTGGAAC ATCCGTTCCG 
GTCGGAAAGA GTATAACCCA ATTGAAAATG ACTGAGCGAG GCACTCAAAC CGGAGTCCAG 
AATCCTTTCT CCGATAATTA CTTTCTCGAA ATGATCAAGC CCCAGATGCA GGAAATGAAC 
CCCCGTCAGA AGATGCACTT TAAGAAGAAA GTATTCCAAG CGCTGATGGA AACCTTTGAT 
GATGCTACCG ATTTCCCAAC GTCTAAAGAA CTGCAGCATT TTAATATAAA CACTCCTTCA 
GGATTTGAGC ACATTACAGA TCCGGAACTT CGATTGGTCA GGGAGCTAGT CAGCATGGTG 
AGCGCGGCTA AGGTTACCCT AATTAGACCG CCAGGAGAAG CTACAGCTAT AGCTACAGCT 
TCCCGCAGTG GAATTGCTCC TGAGGTACAG CGAGGACCCC GAACAAATAT GACACGCCAA 
GTCATTCAGA GGGTATACAA GCCAGGCACT GGACAAGAGA TCGCCCCCAC CAGCCCGGCG 
GGTGGACTAG ATAAGAGGTT GTTCAGGTTA GCGGCTATGG GTGGTAAACC GAATGGCAAC 
TTGAGTGCCA CTCAGGAAAT TTTGCGCAAG GATAGTGTTG ACAGTAACCA TAGTGTGGTC 
AAGGTGGCCA ACCACGCCCC AACAGGCAGT CCCAAAGGAG CTGCTGTAGT GGCTGCTATA 
AGGCCGCAGG GTTCATTGAT CAACAGTTTC TTTGGACAAG GCAATGGACC GACGACGGCC 
AAGGGAGCTG CCTCCGAGAA TATCCGCGCC ATGTCCAGGA GGTATTCCGT GTGCGGTAGC 
AGCAATCCAC CCAATGCCCA AAACGCTAGC CAAGGTTCCG CCAATGGCAG CACCATAAAC 
TCCAATGCTA GCGCTATGGA AGCGTCTATG CTTAAGCGGC GATTGATAGC AGCTGGGCAT 
GGAATGGTGC CACCTACCCA ACGCCCTCGG TATTCTGCCG TAGGTGCTAG TCAAATGACC 
ACTGCATCGC AAGGCAGCAG TTTACTAGTG AGGAAGTCTG TGGGCTGCGT TCCTGCTTAC 
CAAAAGCAAA TCTCACCCAC TGGCGGTGCC TCCCTGCTTC AGAAAACTCC GCAGATTGCC 
AGTGTGCAAG GATCTGCCTT CAATGACTTT GCCCAGCCCA AGCCCGCTGC AACGGCCTCT 
GTAAACGGCG AAGAAAGTTC GCCTACCTCG GCGCTGAAGC GCAGCTTGGT GGTGGCTAAT 
ACCAAAACCT CCTTTCAGGA CCTTCTTCAG CAGGCCAGTC AGACGGTGCA GAGAAATAAA 
ATTGAATCCT CGGAGACAGC AGCCACTATT GCTGCGGATG ATTTTTCGCT GGACAACCTT 
AAGCGAGAGC CAGTGGATCC CGCGGAGGAT CACAATGACA TACTCGGTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261839.1
CDS	178..3291
Translation	MSAASEVNLMLLRSVEKQTALYDRTDENYRKRLPSENAWDMVASEVGESVEKCKRRWRQLRNDYTRWCNADANRRRNGQRRLAYPLADELRFLDRHLNIADDMAADDDRSVSSDKDRDNNRDSEGVDHHAQASLKERASSTSKLVKEVKLASQVRKEKSSQDKRENWENPGDKQRSRKKSAEEKLNDLEESDEPEKVPELDSFLQSDNEDDECMDEEHLEDLEGFDFDLEQSNQEKELTPEKSLEKNNTDSTVSQPEFFVKQTRDPRLLIIGRKNSTSSFAESKASKSISGDPLETAMEDSGDEYGRDGEKRVTGSDTTSPTRRPRRAARASISFAGIRDLPIQKPGQAQRMTRLQRRKSMSLAAVHSVRSSTSPVKMTPVPRSQPINKPPSGPVQITKISHRDDIFPRPAVPSGVVNINQNQLKTQQQQKTLIFPTTDEAIANPQGSGPPQRRSVGRPPKKLPMVQRIVAPETQTKPQSPINTKVLTIGKPTSSVASGNINNIAIKSNVVTSISSSTATTNTITKGTSNNSIKTTENVNVISYSPNSSSTSSSSKATTGASGAVITNIPTTTTAGTSVPVGKSITQLKMTERGTQTGVQNPFSDNYFLEMIKPQMQEMNPRQKMHFKKKVFQALMETFDDATDFPTSKELQHFNINTPSGFEHITDPELRLVRELVSMVSAAKVTLIRPPGEATAIATASRSGIAPEVQRGPRTNMTRQVIQRVYKPGTGQEIAPTSPAGGLDKRLFRLAAMGGKPNGNLSATQEILRKDSVDSNHSVVKVANHAPTGSPKGAAVVAAIRPQGSLINSFFGQGNGPTTAKGAASENIRAMSRRYSVCGSSNPPNAQNASQGSANGSTINSNASAMEASMLKRRLIAAGHGMVPPTQRPRYSAVGASQMTTASQGSSLLVRKSVGCVPAYQKQISPTGGASLLQKTPQIASVQGSAFNDFAQPKPAATASVNGEESSPTSALKRSLVVANTKTSFQDLLQQASQTVQRNKIESSETAATIAADDFSLDNLKREPVDPAEDHNDILGV

Target ORF information:

RefSeq Version	NM_001274910.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stonewall (stwl), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274910.1

ORF Insert Sequence:

ATGAGCGCCG CCTCTGAGGT GAACCTGATG TTGCTGCGGT CAGTGGAGAA GCAGACTGCG 
CTTTATGATC GTACCGACGA AAACTATAGG AAGCGCCTGC CGAGTGAGAA CGCCTGGGAC 
ATGGTTGCCT CCGAAGTTGG AGAGTCGGTG GAAAAATGCA AGCGACGCTG GCGTCAGCTG 
CGAAATGACT ATACCCGCTG GTGCAACGCC GACGCCAACC GGCGACGAAA TGGCCAGCGT 
CGTTTGGCTT ATCCGCTGGC CGATGAGCTG CGCTTTTTGG ACCGCCATCT AAACATAGCT 
GACGACATGG CTGCCGACGA TGACCGCAGC GTATCATCGG ACAAGGACAG GGATAACAAT 
AGAGACAGCG AGGGCGTGGA TCATCATGCT CAGGCGTCAT TAAAAGAACG CGCATCATCG 
ACAAGCAAGT TAGTTAAGGA GGTCAAGCTG GCAAGTCAAG TTCGTAAGGA AAAATCGTCC 
CAAGACAAGA GAGAAAATTG GGAAAATCCT GGAGATAAAC AGCGATCTAG GAAGAAAAGT 
GCTGAAGAGA AGCTGAATGA TTTGGAGGAA TCGGACGAAC CAGAGAAGGT TCCTGAACTC 
GATAGTTTTC TGCAGTCTGA TAATGAAGAC GACGAGTGCA TGGACGAGGA GCACCTAGAA 
GATCTGGAAG GTTTCGATTT TGACTTGGAA CAATCGAATC AGGAAAAGGA GTTAACGCCG 
GAGAAGTCTC TAGAGAAGAA TAACACCGAC TCAACTGTTA GCCAGCCGGA GTTCTTTGTC 
AAGCAAACCA GAGATCCTCG TTTGCTAATT ATTGGCAGAA AGAATTCGAC GTCTTCGTTT 
GCTGAATCCA AGGCTTCAAA ATCTATTTCC GGGGATCCTC TGGAAACGGC TATGGAAGAT 
AGTGGCGATG AATATGGTAG GGATGGTGAA AAACGCGTCA CAGGCAGCGA CACTACTTCT 
CCAACCCGAC GTCCACGTCG CGCAGCCCGT GCATCAATAA GTTTCGCTGG CATAAGGGAT 
TTACCGATCC AGAAACCAGG CCAAGCACAA CGAATGACGC GATTGCAGCG CAGGAAATCT 
ATGAGTTTAG CGGCTGTACA CAGTGTAAGG AGCTCCACAT CGCCTGTGAA AATGACTCCA 
GTCCCCAGGT CTCAGCCGAT CAACAAGCCG CCATCCGGGC CAGTCCAGAT CACGAAAATC 
AGTCATCGGG ATGACATCTT CCCGAGACCT GCGGTGCCAT CTGGAGTTGT GAATATAAAT 
CAAAACCAAC TCAAGACTCA ACAACAGCAG AAGACGCTGA TCTTTCCGAC GACCGATGAA 
GCGATCGCTA ACCCGCAGGG ATCTGGTCCA CCACAAAGGA GGTCCGTGGG TAGGCCGCCC 
AAGAAGCTAC CTATGGTGCA GCGAATAGTT GCGCCGGAAA CGCAGACCAA GCCACAGTCG 
CCCATAAACA CCAAAGTCCT GACCATTGGC AAGCCCACCA GCTCCGTCGC GAGTGGTAAC 
ATAAATAATA TTGCTATCAA AAGCAACGTC GTTACTAGCA TAAGCAGCAG CACCGCTACT 
ACTAATACCA TTACCAAAGG TACCAGTAAC AACAGCATCA AAACTACTGA AAACGTTAAT 
GTAATAAGCT ACAGTCCGAA TTCATCTAGC ACCAGCAGCA GCAGCAAGGC CACCACTGGT 
GCCTCTGGTG CGGTGATAAC TAATATACCC ACAACCACCA CAGCTGGAAC ATCCGTTCCG 
GTCGGAAAGA GTATAACCCA ATTGAAAATG ACTGAGCGAG GCACTCAAAC CGGAGTCCAG 
AATCCTTTCT CCGATAATTA CTTTCTCGAA ATGATCAAGC CCCAGATGCA GGAAATGAAC 
CCCCGTCAGA AGATGCACTT TAAGAAGAAA GTATTCCAAG CGCTGATGGA AACCTTTGAT 
GATGCTACCG ATTTCCCAAC GTCTAAAGAA CTGCAGCATT TTAATATAAA CACTCCTTCA 
GGATTTGAGC ACATTACAGA TCCGGAACTT CGATTGGTCA GGGAGCTAGT CAGCATGGTG 
AGCGCGGCTA AGGTTACCCT AATTAGACCG CCAGGAGAAG CTACAGCTAT AGCTACAGCT 
TCCCGCAGTG GAATTGCTCC TGAGGTACAG CGAGGACCCC GAACAAATAT GACACGCCAA 
GTCATTCAGA GGGTATACAA GCCAGGCACT GGACAAGAGA TCGCCCCCAC CAGCCCGGCG 
GGTGGACTAG ATAAGAGGTT GTTCAGGTTA GCGGCTATGG GTGGTAAACC GAATGGCAAC 
TTGAGTGCCA CTCAGGAAAT TTTGCGCAAG GATAGTGTTG ACAGTAACCA TAGTGTGGTC 
AAGGTGGCCA ACCACGCCCC AACAGGCAGT CCCAAAGGAG CTGCTGTAGT GGCTGCTATA 
AGGCCGCAGG GTTCATTGAT CAACAGTTTC TTTGGACAAG GCAATGGACC GACGACGGCC 
AAGGGAGCTG CCTCCGAGAA TATCCGCGCC ATGTCCAGGA GGTATTCCGT GTGCGGTAGC 
AGCAATCCAC CCAATGCCCA AAACGCTAGC CAAGGTTCCG CCAATGGCAG CACCATAAAC 
TCCAATGCTA GCGCTATGGA AGCGTCTATG CTTAAGCGGC GATTGATAGC AGCTGGGCAT 
GGAATGGTGC CACCTACCCA ACGCCCTCGG TATTCTGCCG TAGGTGCTAG TCAAATGACC 
ACTGCATCGC AAGGCAGCAG TTTACTAGTG AGGAAGTCTG TGGGCTGCGT TCCTGCTTAC 
CAAAAGCAAA TCTCACCCAC TGGCGGTGCC TCCCTGCTTC AGAAAACTCC GCAGATTGCC 
AGTGTGCAAG GATCTGCCTT CAATGACTTT GCCCAGCCCA AGCCCGCTGC AACGGCCTCT 
GTAAACGGCG AAGAAAGTTC GCCTACCTCG GCGCTGAAGC GCAGCTTGGT GGTGGCTAAT 
ACCAAAACCT CCTTTCAGGA CCTTCTTCAG CAGGCCAGTC AGACGGTGCA GAGAAATAAA 
ATTGAATCCT CGGAGACAGC AGCCACTATT GCTGCGGATG ATTTTTCGCT GGACAACCTT 
AAGCGAGAGC CAGTGGATCC CGCGGAGGAT CACAATGACA TACTCGGTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stwl ( NM_079344.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524068.2 Drosophila melanogaster stonewall (stwl), transcript variant A, mRNA.

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CloneID	OFa18496
Clone ID Related Accession (Same CDS sequence)	NM_001274910.1 , NM_079344.3
Accession Version	NM_079344.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stonewall, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079344.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGCCG CCTCTGAGGT GAACCTGATG TTGCTGCGGT CAGTGGAGAA GCAGACTGCG 
CTTTATGATC GTACCGACGA AAACTATAGG AAGCGCCTGC CGAGTGAGAA CGCCTGGGAC 
ATGGTTGCCT CCGAAGTTGG AGAGTCGGTG GAAAAATGCA AGCGACGCTG GCGTCAGCTG 
CGAAATGACT ATACCCGCTG GTGCAACGCC GACGCCAACC GGCGACGAAA TGGCCAGCGT 
CGTTTGGCTT ATCCGCTGGC CGATGAGCTG CGCTTTTTGG ACCGCCATCT AAACATAGCT 
GACGACATGG CTGCCGACGA TGACCGCAGC GTATCATCGG ACAAGGACAG GGATAACAAT 
AGAGACAGCG AGGGCGTGGA TCATCATGCT CAGGCGTCAT TAAAAGAACG CGCATCATCG 
ACAAGCAAGT TAGTTAAGGA GGTCAAGCTG GCAAGTCAAG TTCGTAAGGA AAAATCGTCC 
CAAGACAAGA GAGAAAATTG GGAAAATCCT GGAGATAAAC AGCGATCTAG GAAGAAAAGT 
GCTGAAGAGA AGCTGAATGA TTTGGAGGAA TCGGACGAAC CAGAGAAGGT TCCTGAACTC 
GATAGTTTTC TGCAGTCTGA TAATGAAGAC GACGAGTGCA TGGACGAGGA GCACCTAGAA 
GATCTGGAAG GTTTCGATTT TGACTTGGAA CAATCGAATC AGGAAAAGGA GTTAACGCCG 
GAGAAGTCTC TAGAGAAGAA TAACACCGAC TCAACTGTTA GCCAGCCGGA GTTCTTTGTC 
AAGCAAACCA GAGATCCTCG TTTGCTAATT ATTGGCAGAA AGAATTCGAC GTCTTCGTTT 
GCTGAATCCA AGGCTTCAAA ATCTATTTCC GGGGATCCTC TGGAAACGGC TATGGAAGAT 
AGTGGCGATG AATATGGTAG GGATGGTGAA AAACGCGTCA CAGGCAGCGA CACTACTTCT 
CCAACCCGAC GTCCACGTCG CGCAGCCCGT GCATCAATAA GTTTCGCTGG CATAAGGGAT 
TTACCGATCC AGAAACCAGG CCAAGCACAA CGAATGACGC GATTGCAGCG CAGGAAATCT 
ATGAGTTTAG CGGCTGTACA CAGTGTAAGG AGCTCCACAT CGCCTGTGAA AATGACTCCA 
GTCCCCAGGT CTCAGCCGAT CAACAAGCCG CCATCCGGGC CAGTCCAGAT CACGAAAATC 
AGTCATCGGG ATGACATCTT CCCGAGACCT GCGGTGCCAT CTGGAGTTGT GAATATAAAT 
CAAAACCAAC TCAAGACTCA ACAACAGCAG AAGACGCTGA TCTTTCCGAC GACCGATGAA 
GCGATCGCTA ACCCGCAGGG ATCTGGTCCA CCACAAAGGA GGTCCGTGGG TAGGCCGCCC 
AAGAAGCTAC CTATGGTGCA GCGAATAGTT GCGCCGGAAA CGCAGACCAA GCCACAGTCG 
CCCATAAACA CCAAAGTCCT GACCATTGGC AAGCCCACCA GCTCCGTCGC GAGTGGTAAC 
ATAAATAATA TTGCTATCAA AAGCAACGTC GTTACTAGCA TAAGCAGCAG CACCGCTACT 
ACTAATACCA TTACCAAAGG TACCAGTAAC AACAGCATCA AAACTACTGA AAACGTTAAT 
GTAATAAGCT ACAGTCCGAA TTCATCTAGC ACCAGCAGCA GCAGCAAGGC CACCACTGGT 
GCCTCTGGTG CGGTGATAAC TAATATACCC ACAACCACCA CAGCTGGAAC ATCCGTTCCG 
GTCGGAAAGA GTATAACCCA ATTGAAAATG ACTGAGCGAG GCACTCAAAC CGGAGTCCAG 
AATCCTTTCT CCGATAATTA CTTTCTCGAA ATGATCAAGC CCCAGATGCA GGAAATGAAC 
CCCCGTCAGA AGATGCACTT TAAGAAGAAA GTATTCCAAG CGCTGATGGA AACCTTTGAT 
GATGCTACCG ATTTCCCAAC GTCTAAAGAA CTGCAGCATT TTAATATAAA CACTCCTTCA 
GGATTTGAGC ACATTACAGA TCCGGAACTT CGATTGGTCA GGGAGCTAGT CAGCATGGTG 
AGCGCGGCTA AGGTTACCCT AATTAGACCG CCAGGAGAAG CTACAGCTAT AGCTACAGCT 
TCCCGCAGTG GAATTGCTCC TGAGGTACAG CGAGGACCCC GAACAAATAT GACACGCCAA 
GTCATTCAGA GGGTATACAA GCCAGGCACT GGACAAGAGA TCGCCCCCAC CAGCCCGGCG 
GGTGGACTAG ATAAGAGGTT GTTCAGGTTA GCGGCTATGG GTGGTAAACC GAATGGCAAC 
TTGAGTGCCA CTCAGGAAAT TTTGCGCAAG GATAGTGTTG ACAGTAACCA TAGTGTGGTC 
AAGGTGGCCA ACCACGCCCC AACAGGCAGT CCCAAAGGAG CTGCTGTAGT GGCTGCTATA 
AGGCCGCAGG GTTCATTGAT CAACAGTTTC TTTGGACAAG GCAATGGACC GACGACGGCC 
AAGGGAGCTG CCTCCGAGAA TATCCGCGCC ATGTCCAGGA GGTATTCCGT GTGCGGTAGC 
AGCAATCCAC CCAATGCCCA AAACGCTAGC CAAGGTTCCG CCAATGGCAG CACCATAAAC 
TCCAATGCTA GCGCTATGGA AGCGTCTATG CTTAAGCGGC GATTGATAGC AGCTGGGCAT 
GGAATGGTGC CACCTACCCA ACGCCCTCGG TATTCTGCCG TAGGTGCTAG TCAAATGACC 
ACTGCATCGC AAGGCAGCAG TTTACTAGTG AGGAAGTCTG TGGGCTGCGT TCCTGCTTAC 
CAAAAGCAAA TCTCACCCAC TGGCGGTGCC TCCCTGCTTC AGAAAACTCC GCAGATTGCC 
AGTGTGCAAG GATCTGCCTT CAATGACTTT GCCCAGCCCA AGCCCGCTGC AACGGCCTCT 
GTAAACGGCG AAGAAAGTTC GCCTACCTCG GCGCTGAAGC GCAGCTTGGT GGTGGCTAAT 
ACCAAAACCT CCTTTCAGGA CCTTCTTCAG CAGGCCAGTC AGACGGTGCA GAGAAATAAA 
ATTGAATCCT CGGAGACAGC AGCCACTATT GCTGCGGATG ATTTTTCGCT GGACAACCTT 
AAGCGAGAGC CAGTGGATCC CGCGGAGGAT CACAATGACA TACTCGGTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524068.2
CDS	178..3291
Translation	MSAASEVNLMLLRSVEKQTALYDRTDENYRKRLPSENAWDMVASEVGESVEKCKRRWRQLRNDYTRWCNADANRRRNGQRRLAYPLADELRFLDRHLNIADDMAADDDRSVSSDKDRDNNRDSEGVDHHAQASLKERASSTSKLVKEVKLASQVRKEKSSQDKRENWENPGDKQRSRKKSAEEKLNDLEESDEPEKVPELDSFLQSDNEDDECMDEEHLEDLEGFDFDLEQSNQEKELTPEKSLEKNNTDSTVSQPEFFVKQTRDPRLLIIGRKNSTSSFAESKASKSISGDPLETAMEDSGDEYGRDGEKRVTGSDTTSPTRRPRRAARASISFAGIRDLPIQKPGQAQRMTRLQRRKSMSLAAVHSVRSSTSPVKMTPVPRSQPINKPPSGPVQITKISHRDDIFPRPAVPSGVVNINQNQLKTQQQQKTLIFPTTDEAIANPQGSGPPQRRSVGRPPKKLPMVQRIVAPETQTKPQSPINTKVLTIGKPTSSVASGNINNIAIKSNVVTSISSSTATTNTITKGTSNNSIKTTENVNVISYSPNSSSTSSSSKATTGASGAVITNIPTTTTAGTSVPVGKSITQLKMTERGTQTGVQNPFSDNYFLEMIKPQMQEMNPRQKMHFKKKVFQALMETFDDATDFPTSKELQHFNINTPSGFEHITDPELRLVRELVSMVSAAKVTLIRPPGEATAIATASRSGIAPEVQRGPRTNMTRQVIQRVYKPGTGQEIAPTSPAGGLDKRLFRLAAMGGKPNGNLSATQEILRKDSVDSNHSVVKVANHAPTGSPKGAAVVAAIRPQGSLINSFFGQGNGPTTAKGAASENIRAMSRRYSVCGSSNPPNAQNASQGSANGSTINSNASAMEASMLKRRLIAAGHGMVPPTQRPRYSAVGASQMTTASQGSSLLVRKSVGCVPAYQKQISPTGGASLLQKTPQIASVQGSAFNDFAQPKPAATASVNGEESSPTSALKRSLVVANTKTSFQDLLQQASQTVQRNKIESSETAATIAADDFSLDNLKREPVDPAEDHNDILGV

Target ORF information:

RefSeq Version	NM_079344.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stonewall (stwl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079344.3

ORF Insert Sequence:

ATGAGCGCCG CCTCTGAGGT GAACCTGATG TTGCTGCGGT CAGTGGAGAA GCAGACTGCG 
CTTTATGATC GTACCGACGA AAACTATAGG AAGCGCCTGC CGAGTGAGAA CGCCTGGGAC 
ATGGTTGCCT CCGAAGTTGG AGAGTCGGTG GAAAAATGCA AGCGACGCTG GCGTCAGCTG 
CGAAATGACT ATACCCGCTG GTGCAACGCC GACGCCAACC GGCGACGAAA TGGCCAGCGT 
CGTTTGGCTT ATCCGCTGGC CGATGAGCTG CGCTTTTTGG ACCGCCATCT AAACATAGCT 
GACGACATGG CTGCCGACGA TGACCGCAGC GTATCATCGG ACAAGGACAG GGATAACAAT 
AGAGACAGCG AGGGCGTGGA TCATCATGCT CAGGCGTCAT TAAAAGAACG CGCATCATCG 
ACAAGCAAGT TAGTTAAGGA GGTCAAGCTG GCAAGTCAAG TTCGTAAGGA AAAATCGTCC 
CAAGACAAGA GAGAAAATTG GGAAAATCCT GGAGATAAAC AGCGATCTAG GAAGAAAAGT 
GCTGAAGAGA AGCTGAATGA TTTGGAGGAA TCGGACGAAC CAGAGAAGGT TCCTGAACTC 
GATAGTTTTC TGCAGTCTGA TAATGAAGAC GACGAGTGCA TGGACGAGGA GCACCTAGAA 
GATCTGGAAG GTTTCGATTT TGACTTGGAA CAATCGAATC AGGAAAAGGA GTTAACGCCG 
GAGAAGTCTC TAGAGAAGAA TAACACCGAC TCAACTGTTA GCCAGCCGGA GTTCTTTGTC 
AAGCAAACCA GAGATCCTCG TTTGCTAATT ATTGGCAGAA AGAATTCGAC GTCTTCGTTT 
GCTGAATCCA AGGCTTCAAA ATCTATTTCC GGGGATCCTC TGGAAACGGC TATGGAAGAT 
AGTGGCGATG AATATGGTAG GGATGGTGAA AAACGCGTCA CAGGCAGCGA CACTACTTCT 
CCAACCCGAC GTCCACGTCG CGCAGCCCGT GCATCAATAA GTTTCGCTGG CATAAGGGAT 
TTACCGATCC AGAAACCAGG CCAAGCACAA CGAATGACGC GATTGCAGCG CAGGAAATCT 
ATGAGTTTAG CGGCTGTACA CAGTGTAAGG AGCTCCACAT CGCCTGTGAA AATGACTCCA 
GTCCCCAGGT CTCAGCCGAT CAACAAGCCG CCATCCGGGC CAGTCCAGAT CACGAAAATC 
AGTCATCGGG ATGACATCTT CCCGAGACCT GCGGTGCCAT CTGGAGTTGT GAATATAAAT 
CAAAACCAAC TCAAGACTCA ACAACAGCAG AAGACGCTGA TCTTTCCGAC GACCGATGAA 
GCGATCGCTA ACCCGCAGGG ATCTGGTCCA CCACAAAGGA GGTCCGTGGG TAGGCCGCCC 
AAGAAGCTAC CTATGGTGCA GCGAATAGTT GCGCCGGAAA CGCAGACCAA GCCACAGTCG 
CCCATAAACA CCAAAGTCCT GACCATTGGC AAGCCCACCA GCTCCGTCGC GAGTGGTAAC 
ATAAATAATA TTGCTATCAA AAGCAACGTC GTTACTAGCA TAAGCAGCAG CACCGCTACT 
ACTAATACCA TTACCAAAGG TACCAGTAAC AACAGCATCA AAACTACTGA AAACGTTAAT 
GTAATAAGCT ACAGTCCGAA TTCATCTAGC ACCAGCAGCA GCAGCAAGGC CACCACTGGT 
GCCTCTGGTG CGGTGATAAC TAATATACCC ACAACCACCA CAGCTGGAAC ATCCGTTCCG 
GTCGGAAAGA GTATAACCCA ATTGAAAATG ACTGAGCGAG GCACTCAAAC CGGAGTCCAG 
AATCCTTTCT CCGATAATTA CTTTCTCGAA ATGATCAAGC CCCAGATGCA GGAAATGAAC 
CCCCGTCAGA AGATGCACTT TAAGAAGAAA GTATTCCAAG CGCTGATGGA AACCTTTGAT 
GATGCTACCG ATTTCCCAAC GTCTAAAGAA CTGCAGCATT TTAATATAAA CACTCCTTCA 
GGATTTGAGC ACATTACAGA TCCGGAACTT CGATTGGTCA GGGAGCTAGT CAGCATGGTG 
AGCGCGGCTA AGGTTACCCT AATTAGACCG CCAGGAGAAG CTACAGCTAT AGCTACAGCT 
TCCCGCAGTG GAATTGCTCC TGAGGTACAG CGAGGACCCC GAACAAATAT GACACGCCAA 
GTCATTCAGA GGGTATACAA GCCAGGCACT GGACAAGAGA TCGCCCCCAC CAGCCCGGCG 
GGTGGACTAG ATAAGAGGTT GTTCAGGTTA GCGGCTATGG GTGGTAAACC GAATGGCAAC 
TTGAGTGCCA CTCAGGAAAT TTTGCGCAAG GATAGTGTTG ACAGTAACCA TAGTGTGGTC 
AAGGTGGCCA ACCACGCCCC AACAGGCAGT CCCAAAGGAG CTGCTGTAGT GGCTGCTATA 
AGGCCGCAGG GTTCATTGAT CAACAGTTTC TTTGGACAAG GCAATGGACC GACGACGGCC 
AAGGGAGCTG CCTCCGAGAA TATCCGCGCC ATGTCCAGGA GGTATTCCGT GTGCGGTAGC 
AGCAATCCAC CCAATGCCCA AAACGCTAGC CAAGGTTCCG CCAATGGCAG CACCATAAAC 
TCCAATGCTA GCGCTATGGA AGCGTCTATG CTTAAGCGGC GATTGATAGC AGCTGGGCAT 
GGAATGGTGC CACCTACCCA ACGCCCTCGG TATTCTGCCG TAGGTGCTAG TCAAATGACC 
ACTGCATCGC AAGGCAGCAG TTTACTAGTG AGGAAGTCTG TGGGCTGCGT TCCTGCTTAC 
CAAAAGCAAA TCTCACCCAC TGGCGGTGCC TCCCTGCTTC AGAAAACTCC GCAGATTGCC 
AGTGTGCAAG GATCTGCCTT CAATGACTTT GCCCAGCCCA AGCCCGCTGC AACGGCCTCT 
GTAAACGGCG AAGAAAGTTC GCCTACCTCG GCGCTGAAGC GCAGCTTGGT GGTGGCTAAT 
ACCAAAACCT CCTTTCAGGA CCTTCTTCAG CAGGCCAGTC AGACGGTGCA GAGAAATAAA 
ATTGAATCCT CGGAGACAGC AGCCACTATT GCTGCGGATG ATTTTTCGCT GGACAACCTT 
AAGCGAGAGC CAGTGGATCC CGCGGAGGAT CACAATGACA TACTCGGTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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stwl cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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