Nprl3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nprl3
Entrez Gene ID	39550
Full Name	Nitrogen permease regulator-like 3
Synonyms	CG8783,Dmel\CG8783,NPR3,Nplr3,nprl3
General protein information	Preferred Names Nitrogen permease regulator-like 3 Names CG8783 gene product from transcript CG8783-RB CG8783-PA CG8783-PB CG8783-PC Nprl3-PA Nprl3-PB Nprl3-PC nitrogen permease regulator-like 3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 70C11-70C11

mRNA and Protein(s)

mRNA	Protein	Name
NM_140423.3	NP_648680.2	nitrogen permease regulator-like 3, isoform A
NM_001274889.1	NP_001261818.1	nitrogen permease regulator-like 3, isoform C
NM_168570.1	NP_729945.1	nitrogen permease regulator-like 3, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NPRL3	XP_510703.3
Bos taurus (cattle)	NPRL3	NP_001193244.1
Danio rerio (zebrafish)	nprl3	NP_001020705.2
Drosophila melanogaster (fruit fly)	CG8783	NP_729945.1
Caenorhabditis elegans (roundworm)	nprl-3	NP_501396.1
Homo sapiens (human)	NPRL3	NP_001230177.1
	NPRL3	XP_001118432.2
Canis lupus familiaris (dog)	NPRL3	XP_005621835.1
Mus musculus (house mouse)	Nprl3	NP_001271288.1
Gallus gallus (chicken)	NPRL3	XP_003642182.1
Xenopus tropicalis (tropical clawed frog)	nprl3	NP_001096273.1
Rattus norvegicus (Norway rat)	Nprl3	NP_001030108.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nprl3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nprl3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18465	NM_001274889.1 Latest version!	Drosophila melanogaster nitrogen permease regulator-like 3, transcript variant C (Nprl3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa18465	NM_168570.1 Latest version!	Drosophila melanogaster nitrogen permease regulator-like 3, transcript variant B (Nprl3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa18465	NM_140423.3 Latest version!	Drosophila melanogaster nitrogen permease regulator-like 3, transcript variant A (Nprl3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nprl3 ( NM_140423.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648680.2 Drosophila melanogaster nitrogen permease regulator-like 3, transcript variant A (Nprl3), mRNA.

Price & Availability↑

CloneID	OFa18465
Clone ID Related Accession (Same CDS sequence)	NM_140423.3 , NM_001274889.1 , NM_168570.1
Accession Version	NM_140423.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nitrogen permease regulator-like 3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140423.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAACTA ATGTGAATCC CCTGGCCGTA ATCCTGGTTT ACTTCGATAG CAAGGGGGAT 
CGGTTACTGT ACAGGTATCC GTACCAGACT CTCGGCCAAA CGGAGGTGGC CAACGACGAG 
CAGCGGAAGT CCAGAAAGCG CAATCCCTAT GCCGTGGCCA ATACGGACGA TCTGCTGCAG 
ACACCCACCC ACTTGGGTGC TGCCAAGAGC CAAGGACAAC TGCAGGGATT CGCAGATGAG 
GTCCTGTCCG CTCTGTTTGC TGTTAAACCA CAGCTATGCA ACCAAAAGTT TGAACTCAAA 
CTGAACGACG TACGCTTTGT CAGTCATCCC ACTCTGATAC CGCAAAAGGA GCAGAGAAGT 
GGTCCAATGG CCAAGCAACA GATGCTCATC AACATTGTTT TTGCGCTGCA TGCACAGGCC 
AGCTACTCGA TTGTTAAGTG CTACCACGAA CTGAGCAAGC GCCTTGGACT GGCGCTGAAG 
TTCGAGGAGC AGCGCAGTGG CTATCTCACC GAGCAGACGG CTCAAATGGC TCGCACACAC 
GACGAGCAGC AGCAACAGCC GCTGGAACGC ACACTGGAGC TGATTGCAGA GCGCTGCAGC 
TTGGCACAGG CCTTGCGCTC CATCTTCCAT GATCTATGCA CCACTGGACT GCTGAGTACC 
TCGCTGAATC ACAACCTAAC GTTGTGCTTC TGTCTGCCCG CTAAGGCACA CCAGCTCCAT 
AAGAAGGGCA GCATGGTGGA TCCAGAGACC ATAGATCGGT GCCTTCGTGC ACTGAAGCCC 
TATCATGGCA TGTTGCTGCT GGTCGACTTT GCCGAATTGT TGGACTGCGT TCCACCGACT 
GGGGCACGCA TGCTATGGCA GCTAGTCGAC GTCTACGACC CACTGATCAG TCTGCAGAGC 
ATGTCCTCCA ATGCAGACTT GAGTATCGAG CATGTTTATA AGCTCGTCTC GCATTTGGTC 
TACTGGGCTA AGGCAACTAT TATTTATCCA CTCTGCGAGA CAAATGTCTA TGTGATCGCG 
CCGGATGCAC CATTGCACAC CAAGTCCCAC CTGGTGGAGA AGTTCTCGGC CCGTTTTGCC 
GGTATGTCGC TGTTCGAGGT GATCTCCGAT TTTTCATTGC CCACTTCAAT TGGACACTTA 
ACCACGCCAC TGCAGCAGCC AGCGCGACAG GGTATCCTCG CACAAATGGT GATTTGGATG 
CTGCAGCACC ATTTGCTCAT GCAGTTGCAC ACGTACGTTC AGTTTATGCC CAGCGAGGAT 
GAATTCGGAG ATTCGGCCTC CTGCAGCAAC CATCTAAGGG ATGCCATTAG CGATGAAGAA 
GGAGACCAAG AACCAGATGC GGACGAGTTG CATGGCTCCA TGCTTAGCAT GTCCAGTCAT 
CCGTTGCCAG TGCCAGCGGT ATTGGTGGGC GGACATCGTC GTGAGGCGTC CGAGGACCAT 
TCATCACTGG CCTCGGACAA CATCGCCGTG CAGCCATCAT CCAGCCACAA GTCCAATTTC 
AGCATCACCG CCTCGATGAG CACGGACAAT TGCGACAGCC TCGATTCCAT GGAGGATGAG 
CAGAAGCTGA AGGAGCTGCT ACAGGTTTTC AGCGATGCGG ATCGAGCCGC CATCCGTCGA 
ATTCCCGCCT CTGCCAACGT GGATGATCTA TCTCTGCTTG TGAAGCTGTA TCAGATGGGT 
TACTTTAAGA GCGAGCATCA TCTGGAGGAG ATCATGTACT TCGAGAACCT GCGACGTTCG 
CAACTGCTGC AATTGCTTGA TAAATTCAGG GATGTGCTTA TCATTTACGA AACCGAAGAT 
CCTGCAATTG CATCCATGTA CAATACCAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648680.2
CDS	193..2025
Translation	METNVNPLAVILVYFDSKGDRLLYRYPYQTLGQTEVANDEQRKSRKRNPYAVANTDDLLQTPTHLGAAKSQGQLQGFADEVLSALFAVKPQLCNQKFELKLNDVRFVSHPTLIPQKEQRSGPMAKQQMLINIVFALHAQASYSIVKCYHELSKRLGLALKFEEQRSGYLTEQTAQMARTHDEQQQQPLERTLELIAERCSLAQALRSIFHDLCTTGLLSTSLNHNLTLCFCLPAKAHQLHKKGSMVDPETIDRCLRALKPYHGMLLLVDFAELLDCVPPTGARMLWQLVDVYDPLISLQSMSSNADLSIEHVYKLVSHLVYWAKATIIYPLCETNVYVIAPDAPLHTKSHLVEKFSARFAGMSLFEVISDFSLPTSIGHLTTPLQQPARQGILAQMVIWMLQHHLLMQLHTYVQFMPSEDEFGDSASCSNHLRDAISDEEGDQEPDADELHGSMLSMSSHPLPVPAVLVGGHRREASEDHSSLASDNIAVQPSSSHKSNFSITASMSTDNCDSLDSMEDEQKLKELLQVFSDADRAAIRRIPASANVDDLSLLVKLYQMGYFKSEHHLEEIMYFENLRRSQLLQLLDKFRDVLIIYETEDPAIASMYNTK

Target ORF information:

RefSeq Version	NM_140423.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nitrogen permease regulator-like 3, transcript variant A (Nprl3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140423.3

ORF Insert Sequence:

ATGGAAACTA ATGTGAATCC CCTGGCCGTA ATCCTGGTTT ACTTCGATAG CAAGGGGGAT 
CGGTTACTGT ACAGGTATCC GTACCAGACT CTCGGCCAAA CGGAGGTGGC CAACGACGAG 
CAGCGGAAGT CCAGAAAGCG CAATCCCTAT GCCGTGGCCA ATACGGACGA TCTGCTGCAG 
ACACCCACCC ACTTGGGTGC TGCCAAGAGC CAAGGACAAC TGCAGGGATT CGCAGATGAG 
GTCCTGTCCG CTCTGTTTGC TGTTAAACCA CAGCTATGCA ACCAAAAGTT TGAACTCAAA 
CTGAACGACG TACGCTTTGT CAGTCATCCC ACTCTGATAC CGCAAAAGGA GCAGAGAAGT 
GGTCCAATGG CCAAGCAACA GATGCTCATC AACATTGTTT TTGCGCTGCA TGCACAGGCC 
AGCTACTCGA TTGTTAAGTG CTACCACGAA CTGAGCAAGC GCCTTGGACT GGCGCTGAAG 
TTCGAGGAGC AGCGCAGTGG CTATCTCACC GAGCAGACGG CTCAAATGGC TCGCACACAC 
GACGAGCAGC AGCAACAGCC GCTGGAACGC ACACTGGAGC TGATTGCAGA GCGCTGCAGC 
TTGGCACAGG CCTTGCGCTC CATCTTCCAT GATCTATGCA CCACTGGACT GCTGAGTACC 
TCGCTGAATC ACAACCTAAC GTTGTGCTTC TGTCTGCCCG CTAAGGCACA CCAGCTCCAT 
AAGAAGGGCA GCATGGTGGA TCCAGAGACC ATAGATCGGT GCCTTCGTGC ACTGAAGCCC 
TATCATGGCA TGTTGCTGCT GGTCGACTTT GCCGAATTGT TGGACTGCGT TCCACCGACT 
GGGGCACGCA TGCTATGGCA GCTAGTCGAC GTCTACGACC CACTGATCAG TCTGCAGAGC 
ATGTCCTCCA ATGCAGACTT GAGTATCGAG CATGTTTATA AGCTCGTCTC GCATTTGGTC 
TACTGGGCTA AGGCAACTAT TATTTATCCA CTCTGCGAGA CAAATGTCTA TGTGATCGCG 
CCGGATGCAC CATTGCACAC CAAGTCCCAC CTGGTGGAGA AGTTCTCGGC CCGTTTTGCC 
GGTATGTCGC TGTTCGAGGT GATCTCCGAT TTTTCATTGC CCACTTCAAT TGGACACTTA 
ACCACGCCAC TGCAGCAGCC AGCGCGACAG GGTATCCTCG CACAAATGGT GATTTGGATG 
CTGCAGCACC ATTTGCTCAT GCAGTTGCAC ACGTACGTTC AGTTTATGCC CAGCGAGGAT 
GAATTCGGAG ATTCGGCCTC CTGCAGCAAC CATCTAAGGG ATGCCATTAG CGATGAAGAA 
GGAGACCAAG AACCAGATGC GGACGAGTTG CATGGCTCCA TGCTTAGCAT GTCCAGTCAT 
CCGTTGCCAG TGCCAGCGGT ATTGGTGGGC GGACATCGTC GTGAGGCGTC CGAGGACCAT 
TCATCACTGG CCTCGGACAA CATCGCCGTG CAGCCATCAT CCAGCCACAA GTCCAATTTC 
AGCATCACCG CCTCGATGAG CACGGACAAT TGCGACAGCC TCGATTCCAT GGAGGATGAG 
CAGAAGCTGA AGGAGCTGCT ACAGGTTTTC AGCGATGCGG ATCGAGCCGC CATCCGTCGA 
ATTCCCGCCT CTGCCAACGT GGATGATCTA TCTCTGCTTG TGAAGCTGTA TCAGATGGGT 
TACTTTAAGA GCGAGCATCA TCTGGAGGAG ATCATGTACT TCGAGAACCT GCGACGTTCG 
CAACTGCTGC AATTGCTTGA TAAATTCAGG GATGTGCTTA TCATTTACGA AACCGAAGAT 
CCTGCAATTG CATCCATGTA CAATACCAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nprl3 ( NM_001274889.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261818.1 Drosophila melanogaster nitrogen permease regulator-like 3, transcript variant C (Nprl3), mRNA.

Price & Availability↑

CloneID	OFa18465
Clone ID Related Accession (Same CDS sequence)	NM_140423.3 , NM_001274889.1 , NM_168570.1
Accession Version	NM_001274889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nitrogen permease regulator-like 3, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAACTA ATGTGAATCC CCTGGCCGTA ATCCTGGTTT ACTTCGATAG CAAGGGGGAT 
CGGTTACTGT ACAGGTATCC GTACCAGACT CTCGGCCAAA CGGAGGTGGC CAACGACGAG 
CAGCGGAAGT CCAGAAAGCG CAATCCCTAT GCCGTGGCCA ATACGGACGA TCTGCTGCAG 
ACACCCACCC ACTTGGGTGC TGCCAAGAGC CAAGGACAAC TGCAGGGATT CGCAGATGAG 
GTCCTGTCCG CTCTGTTTGC TGTTAAACCA CAGCTATGCA ACCAAAAGTT TGAACTCAAA 
CTGAACGACG TACGCTTTGT CAGTCATCCC ACTCTGATAC CGCAAAAGGA GCAGAGAAGT 
GGTCCAATGG CCAAGCAACA GATGCTCATC AACATTGTTT TTGCGCTGCA TGCACAGGCC 
AGCTACTCGA TTGTTAAGTG CTACCACGAA CTGAGCAAGC GCCTTGGACT GGCGCTGAAG 
TTCGAGGAGC AGCGCAGTGG CTATCTCACC GAGCAGACGG CTCAAATGGC TCGCACACAC 
GACGAGCAGC AGCAACAGCC GCTGGAACGC ACACTGGAGC TGATTGCAGA GCGCTGCAGC 
TTGGCACAGG CCTTGCGCTC CATCTTCCAT GATCTATGCA CCACTGGACT GCTGAGTACC 
TCGCTGAATC ACAACCTAAC GTTGTGCTTC TGTCTGCCCG CTAAGGCACA CCAGCTCCAT 
AAGAAGGGCA GCATGGTGGA TCCAGAGACC ATAGATCGGT GCCTTCGTGC ACTGAAGCCC 
TATCATGGCA TGTTGCTGCT GGTCGACTTT GCCGAATTGT TGGACTGCGT TCCACCGACT 
GGGGCACGCA TGCTATGGCA GCTAGTCGAC GTCTACGACC CACTGATCAG TCTGCAGAGC 
ATGTCCTCCA ATGCAGACTT GAGTATCGAG CATGTTTATA AGCTCGTCTC GCATTTGGTC 
TACTGGGCTA AGGCAACTAT TATTTATCCA CTCTGCGAGA CAAATGTCTA TGTGATCGCG 
CCGGATGCAC CATTGCACAC CAAGTCCCAC CTGGTGGAGA AGTTCTCGGC CCGTTTTGCC 
GGTATGTCGC TGTTCGAGGT GATCTCCGAT TTTTCATTGC CCACTTCAAT TGGACACTTA 
ACCACGCCAC TGCAGCAGCC AGCGCGACAG GGTATCCTCG CACAAATGGT GATTTGGATG 
CTGCAGCACC ATTTGCTCAT GCAGTTGCAC ACGTACGTTC AGTTTATGCC CAGCGAGGAT 
GAATTCGGAG ATTCGGCCTC CTGCAGCAAC CATCTAAGGG ATGCCATTAG CGATGAAGAA 
GGAGACCAAG AACCAGATGC GGACGAGTTG CATGGCTCCA TGCTTAGCAT GTCCAGTCAT 
CCGTTGCCAG TGCCAGCGGT ATTGGTGGGC GGACATCGTC GTGAGGCGTC CGAGGACCAT 
TCATCACTGG CCTCGGACAA CATCGCCGTG CAGCCATCAT CCAGCCACAA GTCCAATTTC 
AGCATCACCG CCTCGATGAG CACGGACAAT TGCGACAGCC TCGATTCCAT GGAGGATGAG 
CAGAAGCTGA AGGAGCTGCT ACAGGTTTTC AGCGATGCGG ATCGAGCCGC CATCCGTCGA 
ATTCCCGCCT CTGCCAACGT GGATGATCTA TCTCTGCTTG TGAAGCTGTA TCAGATGGGT 
TACTTTAAGA GCGAGCATCA TCTGGAGGAG ATCATGTACT TCGAGAACCT GCGACGTTCG 
CAACTGCTGC AATTGCTTGA TAAATTCAGG GATGTGCTTA TCATTTACGA AACCGAAGAT 
CCTGCAATTG CATCCATGTA CAATACCAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261818.1
CDS	39..1871
Translation	METNVNPLAVILVYFDSKGDRLLYRYPYQTLGQTEVANDEQRKSRKRNPYAVANTDDLLQTPTHLGAAKSQGQLQGFADEVLSALFAVKPQLCNQKFELKLNDVRFVSHPTLIPQKEQRSGPMAKQQMLINIVFALHAQASYSIVKCYHELSKRLGLALKFEEQRSGYLTEQTAQMARTHDEQQQQPLERTLELIAERCSLAQALRSIFHDLCTTGLLSTSLNHNLTLCFCLPAKAHQLHKKGSMVDPETIDRCLRALKPYHGMLLLVDFAELLDCVPPTGARMLWQLVDVYDPLISLQSMSSNADLSIEHVYKLVSHLVYWAKATIIYPLCETNVYVIAPDAPLHTKSHLVEKFSARFAGMSLFEVISDFSLPTSIGHLTTPLQQPARQGILAQMVIWMLQHHLLMQLHTYVQFMPSEDEFGDSASCSNHLRDAISDEEGDQEPDADELHGSMLSMSSHPLPVPAVLVGGHRREASEDHSSLASDNIAVQPSSSHKSNFSITASMSTDNCDSLDSMEDEQKLKELLQVFSDADRAAIRRIPASANVDDLSLLVKLYQMGYFKSEHHLEEIMYFENLRRSQLLQLLDKFRDVLIIYETEDPAIASMYNTK

Target ORF information:

RefSeq Version	NM_001274889.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nitrogen permease regulator-like 3, transcript variant C (Nprl3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274889.1

ORF Insert Sequence:

ATGGAAACTA ATGTGAATCC CCTGGCCGTA ATCCTGGTTT ACTTCGATAG CAAGGGGGAT 
CGGTTACTGT ACAGGTATCC GTACCAGACT CTCGGCCAAA CGGAGGTGGC CAACGACGAG 
CAGCGGAAGT CCAGAAAGCG CAATCCCTAT GCCGTGGCCA ATACGGACGA TCTGCTGCAG 
ACACCCACCC ACTTGGGTGC TGCCAAGAGC CAAGGACAAC TGCAGGGATT CGCAGATGAG 
GTCCTGTCCG CTCTGTTTGC TGTTAAACCA CAGCTATGCA ACCAAAAGTT TGAACTCAAA 
CTGAACGACG TACGCTTTGT CAGTCATCCC ACTCTGATAC CGCAAAAGGA GCAGAGAAGT 
GGTCCAATGG CCAAGCAACA GATGCTCATC AACATTGTTT TTGCGCTGCA TGCACAGGCC 
AGCTACTCGA TTGTTAAGTG CTACCACGAA CTGAGCAAGC GCCTTGGACT GGCGCTGAAG 
TTCGAGGAGC AGCGCAGTGG CTATCTCACC GAGCAGACGG CTCAAATGGC TCGCACACAC 
GACGAGCAGC AGCAACAGCC GCTGGAACGC ACACTGGAGC TGATTGCAGA GCGCTGCAGC 
TTGGCACAGG CCTTGCGCTC CATCTTCCAT GATCTATGCA CCACTGGACT GCTGAGTACC 
TCGCTGAATC ACAACCTAAC GTTGTGCTTC TGTCTGCCCG CTAAGGCACA CCAGCTCCAT 
AAGAAGGGCA GCATGGTGGA TCCAGAGACC ATAGATCGGT GCCTTCGTGC ACTGAAGCCC 
TATCATGGCA TGTTGCTGCT GGTCGACTTT GCCGAATTGT TGGACTGCGT TCCACCGACT 
GGGGCACGCA TGCTATGGCA GCTAGTCGAC GTCTACGACC CACTGATCAG TCTGCAGAGC 
ATGTCCTCCA ATGCAGACTT GAGTATCGAG CATGTTTATA AGCTCGTCTC GCATTTGGTC 
TACTGGGCTA AGGCAACTAT TATTTATCCA CTCTGCGAGA CAAATGTCTA TGTGATCGCG 
CCGGATGCAC CATTGCACAC CAAGTCCCAC CTGGTGGAGA AGTTCTCGGC CCGTTTTGCC 
GGTATGTCGC TGTTCGAGGT GATCTCCGAT TTTTCATTGC CCACTTCAAT TGGACACTTA 
ACCACGCCAC TGCAGCAGCC AGCGCGACAG GGTATCCTCG CACAAATGGT GATTTGGATG 
CTGCAGCACC ATTTGCTCAT GCAGTTGCAC ACGTACGTTC AGTTTATGCC CAGCGAGGAT 
GAATTCGGAG ATTCGGCCTC CTGCAGCAAC CATCTAAGGG ATGCCATTAG CGATGAAGAA 
GGAGACCAAG AACCAGATGC GGACGAGTTG CATGGCTCCA TGCTTAGCAT GTCCAGTCAT 
CCGTTGCCAG TGCCAGCGGT ATTGGTGGGC GGACATCGTC GTGAGGCGTC CGAGGACCAT 
TCATCACTGG CCTCGGACAA CATCGCCGTG CAGCCATCAT CCAGCCACAA GTCCAATTTC 
AGCATCACCG CCTCGATGAG CACGGACAAT TGCGACAGCC TCGATTCCAT GGAGGATGAG 
CAGAAGCTGA AGGAGCTGCT ACAGGTTTTC AGCGATGCGG ATCGAGCCGC CATCCGTCGA 
ATTCCCGCCT CTGCCAACGT GGATGATCTA TCTCTGCTTG TGAAGCTGTA TCAGATGGGT 
TACTTTAAGA GCGAGCATCA TCTGGAGGAG ATCATGTACT TCGAGAACCT GCGACGTTCG 
CAACTGCTGC AATTGCTTGA TAAATTCAGG GATGTGCTTA TCATTTACGA AACCGAAGAT 
CCTGCAATTG CATCCATGTA CAATACCAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nprl3 ( NM_168570.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729945.1 Drosophila melanogaster nitrogen permease regulator-like 3, transcript variant B (Nprl3), mRNA.

Price & Availability↑

CloneID	OFa18465
Clone ID Related Accession (Same CDS sequence)	NM_140423.3 , NM_001274889.1 , NM_168570.1
Accession Version	NM_168570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nitrogen permease regulator-like 3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAACTA ATGTGAATCC CCTGGCCGTA ATCCTGGTTT ACTTCGATAG CAAGGGGGAT 
CGGTTACTGT ACAGGTATCC GTACCAGACT CTCGGCCAAA CGGAGGTGGC CAACGACGAG 
CAGCGGAAGT CCAGAAAGCG CAATCCCTAT GCCGTGGCCA ATACGGACGA TCTGCTGCAG 
ACACCCACCC ACTTGGGTGC TGCCAAGAGC CAAGGACAAC TGCAGGGATT CGCAGATGAG 
GTCCTGTCCG CTCTGTTTGC TGTTAAACCA CAGCTATGCA ACCAAAAGTT TGAACTCAAA 
CTGAACGACG TACGCTTTGT CAGTCATCCC ACTCTGATAC CGCAAAAGGA GCAGAGAAGT 
GGTCCAATGG CCAAGCAACA GATGCTCATC AACATTGTTT TTGCGCTGCA TGCACAGGCC 
AGCTACTCGA TTGTTAAGTG CTACCACGAA CTGAGCAAGC GCCTTGGACT GGCGCTGAAG 
TTCGAGGAGC AGCGCAGTGG CTATCTCACC GAGCAGACGG CTCAAATGGC TCGCACACAC 
GACGAGCAGC AGCAACAGCC GCTGGAACGC ACACTGGAGC TGATTGCAGA GCGCTGCAGC 
TTGGCACAGG CCTTGCGCTC CATCTTCCAT GATCTATGCA CCACTGGACT GCTGAGTACC 
TCGCTGAATC ACAACCTAAC GTTGTGCTTC TGTCTGCCCG CTAAGGCACA CCAGCTCCAT 
AAGAAGGGCA GCATGGTGGA TCCAGAGACC ATAGATCGGT GCCTTCGTGC ACTGAAGCCC 
TATCATGGCA TGTTGCTGCT GGTCGACTTT GCCGAATTGT TGGACTGCGT TCCACCGACT 
GGGGCACGCA TGCTATGGCA GCTAGTCGAC GTCTACGACC CACTGATCAG TCTGCAGAGC 
ATGTCCTCCA ATGCAGACTT GAGTATCGAG CATGTTTATA AGCTCGTCTC GCATTTGGTC 
TACTGGGCTA AGGCAACTAT TATTTATCCA CTCTGCGAGA CAAATGTCTA TGTGATCGCG 
CCGGATGCAC CATTGCACAC CAAGTCCCAC CTGGTGGAGA AGTTCTCGGC CCGTTTTGCC 
GGTATGTCGC TGTTCGAGGT GATCTCCGAT TTTTCATTGC CCACTTCAAT TGGACACTTA 
ACCACGCCAC TGCAGCAGCC AGCGCGACAG GGTATCCTCG CACAAATGGT GATTTGGATG 
CTGCAGCACC ATTTGCTCAT GCAGTTGCAC ACGTACGTTC AGTTTATGCC CAGCGAGGAT 
GAATTCGGAG ATTCGGCCTC CTGCAGCAAC CATCTAAGGG ATGCCATTAG CGATGAAGAA 
GGAGACCAAG AACCAGATGC GGACGAGTTG CATGGCTCCA TGCTTAGCAT GTCCAGTCAT 
CCGTTGCCAG TGCCAGCGGT ATTGGTGGGC GGACATCGTC GTGAGGCGTC CGAGGACCAT 
TCATCACTGG CCTCGGACAA CATCGCCGTG CAGCCATCAT CCAGCCACAA GTCCAATTTC 
AGCATCACCG CCTCGATGAG CACGGACAAT TGCGACAGCC TCGATTCCAT GGAGGATGAG 
CAGAAGCTGA AGGAGCTGCT ACAGGTTTTC AGCGATGCGG ATCGAGCCGC CATCCGTCGA 
ATTCCCGCCT CTGCCAACGT GGATGATCTA TCTCTGCTTG TGAAGCTGTA TCAGATGGGT 
TACTTTAAGA GCGAGCATCA TCTGGAGGAG ATCATGTACT TCGAGAACCT GCGACGTTCG 
CAACTGCTGC AATTGCTTGA TAAATTCAGG GATGTGCTTA TCATTTACGA AACCGAAGAT 
CCTGCAATTG CATCCATGTA CAATACCAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729945.1
CDS	110..1942
Translation	METNVNPLAVILVYFDSKGDRLLYRYPYQTLGQTEVANDEQRKSRKRNPYAVANTDDLLQTPTHLGAAKSQGQLQGFADEVLSALFAVKPQLCNQKFELKLNDVRFVSHPTLIPQKEQRSGPMAKQQMLINIVFALHAQASYSIVKCYHELSKRLGLALKFEEQRSGYLTEQTAQMARTHDEQQQQPLERTLELIAERCSLAQALRSIFHDLCTTGLLSTSLNHNLTLCFCLPAKAHQLHKKGSMVDPETIDRCLRALKPYHGMLLLVDFAELLDCVPPTGARMLWQLVDVYDPLISLQSMSSNADLSIEHVYKLVSHLVYWAKATIIYPLCETNVYVIAPDAPLHTKSHLVEKFSARFAGMSLFEVISDFSLPTSIGHLTTPLQQPARQGILAQMVIWMLQHHLLMQLHTYVQFMPSEDEFGDSASCSNHLRDAISDEEGDQEPDADELHGSMLSMSSHPLPVPAVLVGGHRREASEDHSSLASDNIAVQPSSSHKSNFSITASMSTDNCDSLDSMEDEQKLKELLQVFSDADRAAIRRIPASANVDDLSLLVKLYQMGYFKSEHHLEEIMYFENLRRSQLLQLLDKFRDVLIIYETEDPAIASMYNTK

Target ORF information:

RefSeq Version	NM_168570.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nitrogen permease regulator-like 3, transcript variant B (Nprl3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168570.1

ORF Insert Sequence:

ATGGAAACTA ATGTGAATCC CCTGGCCGTA ATCCTGGTTT ACTTCGATAG CAAGGGGGAT 
CGGTTACTGT ACAGGTATCC GTACCAGACT CTCGGCCAAA CGGAGGTGGC CAACGACGAG 
CAGCGGAAGT CCAGAAAGCG CAATCCCTAT GCCGTGGCCA ATACGGACGA TCTGCTGCAG 
ACACCCACCC ACTTGGGTGC TGCCAAGAGC CAAGGACAAC TGCAGGGATT CGCAGATGAG 
GTCCTGTCCG CTCTGTTTGC TGTTAAACCA CAGCTATGCA ACCAAAAGTT TGAACTCAAA 
CTGAACGACG TACGCTTTGT CAGTCATCCC ACTCTGATAC CGCAAAAGGA GCAGAGAAGT 
GGTCCAATGG CCAAGCAACA GATGCTCATC AACATTGTTT TTGCGCTGCA TGCACAGGCC 
AGCTACTCGA TTGTTAAGTG CTACCACGAA CTGAGCAAGC GCCTTGGACT GGCGCTGAAG 
TTCGAGGAGC AGCGCAGTGG CTATCTCACC GAGCAGACGG CTCAAATGGC TCGCACACAC 
GACGAGCAGC AGCAACAGCC GCTGGAACGC ACACTGGAGC TGATTGCAGA GCGCTGCAGC 
TTGGCACAGG CCTTGCGCTC CATCTTCCAT GATCTATGCA CCACTGGACT GCTGAGTACC 
TCGCTGAATC ACAACCTAAC GTTGTGCTTC TGTCTGCCCG CTAAGGCACA CCAGCTCCAT 
AAGAAGGGCA GCATGGTGGA TCCAGAGACC ATAGATCGGT GCCTTCGTGC ACTGAAGCCC 
TATCATGGCA TGTTGCTGCT GGTCGACTTT GCCGAATTGT TGGACTGCGT TCCACCGACT 
GGGGCACGCA TGCTATGGCA GCTAGTCGAC GTCTACGACC CACTGATCAG TCTGCAGAGC 
ATGTCCTCCA ATGCAGACTT GAGTATCGAG CATGTTTATA AGCTCGTCTC GCATTTGGTC 
TACTGGGCTA AGGCAACTAT TATTTATCCA CTCTGCGAGA CAAATGTCTA TGTGATCGCG 
CCGGATGCAC CATTGCACAC CAAGTCCCAC CTGGTGGAGA AGTTCTCGGC CCGTTTTGCC 
GGTATGTCGC TGTTCGAGGT GATCTCCGAT TTTTCATTGC CCACTTCAAT TGGACACTTA 
ACCACGCCAC TGCAGCAGCC AGCGCGACAG GGTATCCTCG CACAAATGGT GATTTGGATG 
CTGCAGCACC ATTTGCTCAT GCAGTTGCAC ACGTACGTTC AGTTTATGCC CAGCGAGGAT 
GAATTCGGAG ATTCGGCCTC CTGCAGCAAC CATCTAAGGG ATGCCATTAG CGATGAAGAA 
GGAGACCAAG AACCAGATGC GGACGAGTTG CATGGCTCCA TGCTTAGCAT GTCCAGTCAT 
CCGTTGCCAG TGCCAGCGGT ATTGGTGGGC GGACATCGTC GTGAGGCGTC CGAGGACCAT 
TCATCACTGG CCTCGGACAA CATCGCCGTG CAGCCATCAT CCAGCCACAA GTCCAATTTC 
AGCATCACCG CCTCGATGAG CACGGACAAT TGCGACAGCC TCGATTCCAT GGAGGATGAG 
CAGAAGCTGA AGGAGCTGCT ACAGGTTTTC AGCGATGCGG ATCGAGCCGC CATCCGTCGA 
ATTCCCGCCT CTGCCAACGT GGATGATCTA TCTCTGCTTG TGAAGCTGTA TCAGATGGGT 
TACTTTAAGA GCGAGCATCA TCTGGAGGAG ATCATGTACT TCGAGAACCT GCGACGTTCG 
CAACTGCTGC AATTGCTTGA TAAATTCAGG GATGTGCTTA TCATTTACGA AACCGAAGAT 
CCTGCAATTG CATCCATGTA CAATACCAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nprl3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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