IRSp53 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	IRSp53
Entrez Gene ID	39258
Full Name	CG32082 gene product from transcript CG32082-RA
Synonyms	CG32082,CG7624,Dmel\CG32082,anon-WO0118547.199
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 68A8-68A9

mRNA and Protein(s)

mRNA	Protein	Name
NM_001104113.2	NP_001097583.2	insulin receptor substrate 53 kDa, isoform D
NM_168448.3	NP_729679.2	insulin receptor substrate 53 kDa, isoform A
NM_001300098.1	NP_001287027.1	insulin receptor substrate 53 kDa, isoform E
NM_001104112.2	NP_001097582.1	insulin receptor substrate 53 kDa, isoform B

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100485322	XP_002935048.2
Drosophila melanogaster (fruit fly)	CG32082	NP_729679.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following IRSp53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IRSp53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18221	NM_001104112.2 Latest version!	Drosophila melanogaster insulin receptor substrate 53 kDa, transcript variant B (IRSp53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa18222	NM_001104113.2 Latest version!	Drosophila melanogaster insulin receptor substrate 53 kDa, transcript variant D (IRSp53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa22007	NM_001300098.1 Latest version!	Drosophila melanogaster insulin receptor substrate 53 kDa, transcript variant E (IRSp53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$342.30 $489.00
OFa18220	NM_168448.3 Latest version!	Drosophila melanogaster insulin receptor substrate 53 kDa, transcript variant A (IRSp53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IRSp53 ( NM_001104112.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097582.1 Drosophila melanogaster insulin receptor substrate 53 kDa, transcript variant B (IRSp53), mRNA.

Price & Availability↑

CloneID	OFa18221
Clone ID Related Accession (Same CDS sequence)	NM_001104112.2
Accession Version	NM_001104112.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	insulin receptor substrate 53 kDa, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001104112.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGCCG AGGAAGTTAC AAAACTCGTC GATGGCGTGT ATCGGAACAT ACTGGATAGA 
TTCAATCCGG GTGCCAGGCA GCTGATCGCA GCGGGCAAAA GTTACCTCAA GGCGTTGCAC 
GGTGCCGCAA CCGCCTCGCG TCTATTCAAT GAAGCATTGG CCAAGATTGC GATGAACGCC 
CAGCAAAGCG GAACGGGCGA TATTGGTTCA GCTTTGATGA GCGTTGTGAA TGTGAACAAG 
GAGATACAGG ATCAGCAGAT GAATATTCTG AAAGCCTTCT ACGTGGATCT ATTGGTGCCA 
TTGGAGACAA ATCTGGAGAA GGACACAAAG GTGGTGCAGC ATGAGCAAAA GAAGTTCCTG 
CAGCAGCACA AAGTGCGAAT GGAGAGCTAT CAGAAGGCCG TGTCCACGAT GAAGAAGCAG 
CGCAAGAAAA AGGCCACGCC CGAGAACACC GAAAAGGAGC TTAGGAGCCT CCAATTGCTC 
GAGGATCAGA AGAAAAAGCT GGATGTGTTC TGCGATCAGA GCTACAAGAA TGCCATGACA 
CAGGAGCGAC GACGGTACGG ATTCGTCTTG GAGCGCCAGT GCTCGATCGC CAAACACTGG 
ATGGTCTACC ACACCACCGG CAAGACGGTA ATTGATAATA ATTTCGAAAA TTGGCAAGAG 
ATTGCCGCTT CTCGTGAGAT CATTCCACCG GCGGCCTATG AAAGTGGATA CAGTACCAGC 
AACGGACACA ACAACAACAA CATCAGCAGC AGCAGCAGCA GCGGCAACAA CAACACCAGC 
GGCAACATGC GACGCCTGGA ACGAATTAAG GACGGCGACG ATGACCACCT CCAGGGTCAC 
GGAGCTCAGC TGAAGAAATC GCGCAGCATC GATGCCCCCT ATGGTGATAT GCGCACCCTG 
CACGAACGGG AGAACCTTTC CGGATTGGGC GGTACTGGGC CAGCGCACTA TGCCCAGAAC 
TCGCTGCCAC GCGCCAAATC TGATTTCAAT CTGGCTTTGG TGGGCACTGG ACAGAGCTCA 
GCCAACAAAC ACAAGATCAT TGAGGAGCAG CTAGCCACTC TGGGCGACGA TCAGCGCGGT 
GATCAGCGTC CTCTGGTGAA GGCCCTCTAC GCCTACATGC CCTCCGGCGA GAACCAGTTG 
TCCTTCGAGG AGGGCGACCG CATTGCTTTG GTGGGCGGAA AGGCCAAGGG CTGGCAATTC 
GGTGAGAATC TGCGGACACA GCACTTCGGC TGGTTTCCGG TGGCCTACAC CAATGCGGAA 
GTGGCAGAGG TGACCACTTC CGGTGGTCGG CGATACGAAA ACATGCTGAT GAGCTATGAG 
CGAAATGGTG GCGGATCAGG ATCCGGTTCT GGCGGAGGTG GTCTGCGTAG CTATCAGCAA 
CAGCAGGATT ACGAGGCGCA GCTGGAGCTG ATGGAGAGCC ATGAGGCAAC GTACCGCAGG 
CGAAGGAATC ACAGTGCCGA GGATTCATCA CCCACGCGGA TGTTCGGCGA CACAATCAAG 
CAGCAGAAGA AGTACCGCCC CGGATCCGGG GCCAATCCGC GACCGGGACC GCCACCCACA 
CTGCCCGCTC CGGTGCCCAA TGGACCGCAG AGCCAGTCTT CCAGAATGCT GAACAGCTCG 
CAAAGCTTCT GCGCCTCCAA CGGAGGAGGA GTCAATGCGG CGGTGGAAAG ACGCAAACAA 
AAGCTACTTA ATGGCGTTCA GAGCTCGAAG AACCATCCAT CGGGCAAATC CGGAGTGGCG 
GCCAAGACCT CGCTGCATAG CAGCAATGAC AGTGGATTCG CCAACGAGCC TCCACCACAG 
CCGGAAGTTG ACTATTCGGA TGAGGAGCCA GCCACCCAAA GAGTGGCCAT TCGCAGTCGC 
GGTATATATC GCAACTATTC CATAGATTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097582.1
CDS	881..2773
Translation	MEAEEVTKLVDGVYRNILDRFNPGARQLIAAGKSYLKALHGAATASRLFNEALAKIAMNAQQSGTGDIGSALMSVVNVNKEIQDQQMNILKAFYVDLLVPLETNLEKDTKVVQHEQKKFLQQHKVRMESYQKAVSTMKKQRKKKATPENTEKELRSLQLLEDQKKKLDVFCDQSYKNAMTQERRRYGFVLERQCSIAKHWMVYHTTGKTVIDNNFENWQEIAASREIIPPAAYESGYSTSNGHNNNNISSSSSSGNNNTSGNMRRLERIKDGDDDHLQGHGAQLKKSRSIDAPYGDMRTLHERENLSGLGGTGPAHYAQNSLPRAKSDFNLALVGTGQSSANKHKIIEEQLATLGDDQRGDQRPLVKALYAYMPSGENQLSFEEGDRIALVGGKAKGWQFGENLRTQHFGWFPVAYTNAEVAEVTTSGGRRYENMLMSYERNGGGSGSGSGGGGLRSYQQQQDYEAQLELMESHEATYRRRRNHSAEDSSPTRMFGDTIKQQKKYRPGSGANPRPGPPPTLPAPVPNGPQSQSSRMLNSSQSFCASNGGGVNAAVERRKQKLLNGVQSSKNHPSGKSGVAAKTSLHSSNDSGFANEPPPQPEVDYSDEEPATQRVAIRSRGIYRNYSIDY

Target ORF information:

RefSeq Version	NM_001104112.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster insulin receptor substrate 53 kDa, transcript variant B (IRSp53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104112.2

ORF Insert Sequence:

ATGGAGGCCG AGGAAGTTAC AAAACTCGTC GATGGCGTGT ATCGGAACAT ACTGGATAGA 
TTCAATCCGG GTGCCAGGCA GCTGATCGCA GCGGGCAAAA GTTACCTCAA GGCGTTGCAC 
GGTGCCGCAA CCGCCTCGCG TCTATTCAAT GAAGCATTGG CCAAGATTGC GATGAACGCC 
CAGCAAAGCG GAACGGGCGA TATTGGTTCA GCTTTGATGA GCGTTGTGAA TGTGAACAAG 
GAGATACAGG ATCAGCAGAT GAATATTCTG AAAGCCTTCT ACGTGGATCT ATTGGTGCCA 
TTGGAGACAA ATCTGGAGAA GGACACAAAG GTGGTGCAGC ATGAGCAAAA GAAGTTCCTG 
CAGCAGCACA AAGTGCGAAT GGAGAGCTAT CAGAAGGCCG TGTCCACGAT GAAGAAGCAG 
CGCAAGAAAA AGGCCACGCC CGAGAACACC GAAAAGGAGC TTAGGAGCCT CCAATTGCTC 
GAGGATCAGA AGAAAAAGCT GGATGTGTTC TGCGATCAGA GCTACAAGAA TGCCATGACA 
CAGGAGCGAC GACGGTACGG ATTCGTCTTG GAGCGCCAGT GCTCGATCGC CAAACACTGG 
ATGGTCTACC ACACCACCGG CAAGACGGTA ATTGATAATA ATTTCGAAAA TTGGCAAGAG 
ATTGCCGCTT CTCGTGAGAT CATTCCACCG GCGGCCTATG AAAGTGGATA CAGTACCAGC 
AACGGACACA ACAACAACAA CATCAGCAGC AGCAGCAGCA GCGGCAACAA CAACACCAGC 
GGCAACATGC GACGCCTGGA ACGAATTAAG GACGGCGACG ATGACCACCT CCAGGGTCAC 
GGAGCTCAGC TGAAGAAATC GCGCAGCATC GATGCCCCCT ATGGTGATAT GCGCACCCTG 
CACGAACGGG AGAACCTTTC CGGATTGGGC GGTACTGGGC CAGCGCACTA TGCCCAGAAC 
TCGCTGCCAC GCGCCAAATC TGATTTCAAT CTGGCTTTGG TGGGCACTGG ACAGAGCTCA 
GCCAACAAAC ACAAGATCAT TGAGGAGCAG CTAGCCACTC TGGGCGACGA TCAGCGCGGT 
GATCAGCGTC CTCTGGTGAA GGCCCTCTAC GCCTACATGC CCTCCGGCGA GAACCAGTTG 
TCCTTCGAGG AGGGCGACCG CATTGCTTTG GTGGGCGGAA AGGCCAAGGG CTGGCAATTC 
GGTGAGAATC TGCGGACACA GCACTTCGGC TGGTTTCCGG TGGCCTACAC CAATGCGGAA 
GTGGCAGAGG TGACCACTTC CGGTGGTCGG CGATACGAAA ACATGCTGAT GAGCTATGAG 
CGAAATGGTG GCGGATCAGG ATCCGGTTCT GGCGGAGGTG GTCTGCGTAG CTATCAGCAA 
CAGCAGGATT ACGAGGCGCA GCTGGAGCTG ATGGAGAGCC ATGAGGCAAC GTACCGCAGG 
CGAAGGAATC ACAGTGCCGA GGATTCATCA CCCACGCGGA TGTTCGGCGA CACAATCAAG 
CAGCAGAAGA AGTACCGCCC CGGATCCGGG GCCAATCCGC GACCGGGACC GCCACCCACA 
CTGCCCGCTC CGGTGCCCAA TGGACCGCAG AGCCAGTCTT CCAGAATGCT GAACAGCTCG 
CAAAGCTTCT GCGCCTCCAA CGGAGGAGGA GTCAATGCGG CGGTGGAAAG ACGCAAACAA 
AAGCTACTTA ATGGCGTTCA GAGCTCGAAG AACCATCCAT CGGGCAAATC CGGAGTGGCG 
GCCAAGACCT CGCTGCATAG CAGCAATGAC AGTGGATTCG CCAACGAGCC TCCACCACAG 
CCGGAAGTTG ACTATTCGGA TGAGGAGCCA GCCACCCAAA GAGTGGCCAT TCGCAGTCGC 
GGTATATATC GCAACTATTC CATAGATTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IRSp53 ( NM_001104113.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097583.2 Drosophila melanogaster insulin receptor substrate 53 kDa, transcript variant D (IRSp53), mRNA.

Price & Availability↑

CloneID	OFa18222
Clone ID Related Accession (Same CDS sequence)	NM_001104113.2
Accession Version	NM_001104113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	insulin receptor substrate 53 kDa, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001104113.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGCCG AGGAAGTTAC AAAACTCGTC GATGGCGTGT ATCGGAACAT ACTGGATAGA 
TTCAATCCGG GTGCCAGGCA GCTGATCGCA GCGGGCAAAA GTTACCTCAA GGCGTTGCAC 
GGTGCCGCAA CCGCCTCGCG TCTATTCAAT GAAGCATTGG CCAAGATTGC GATGAACGCC 
CAGCAAAGCG GAACGGGCGA TATTGGTTCA GCTTTGATGA GCGTTGTGAA TGTGAACAAG 
GAGATACAGG ATCAGCAGAT GAATATTCTG AAAGCCTTCT ACGTGGATCT ATTGGTGCCA 
TTGGAGACAA ATCTGGAGAA GGACACAAAG GTGGTGCAGC ATGAGCAAAA GAAGTTCCTG 
CAGCAGCACA AAGTGCGAAT GGAGAGCTAT CAGAAGGCCG TGTCCACGAT GAAGAAGCAG 
CGCAAGAAAA AGGCCACGCC CGAGAACACC GAAAAGGAGC TTAGGAGCCT CCAATTGCTC 
GAGGATCAGA AGAAAAAGCT GGATGTGTTC TGCGATCAGA GCTACAAGAA TGCCATGACA 
CAGGAGCGAC GACGGTACGG ATTCGTCTTG GAGCGCCAGT GCTCGATCGC CAAACACTGG 
ATGGTCTACC ACACCACCGG CAAGACGGTA ATTGATAATA ATTTCGAAAA TTGGCAAGAG 
ATTGCCGCTT CTCGTGAGAT CATTCCACCG GCGGCCTATG AAAGTGGATA CAGTACCAGC 
AACGGACACA ACAACAACAA CATCAGCAGC AGCAGCAGCA GCGGCAACAA CAACACCAGC 
GGCAACATGC GACGCCTGGA ACGAATTAAG GACGGCGACG ATGACCACCT CCAGGGTCAC 
GGAGCTCAGC TGAAGAAATC GCGCAGCATC GATGCCCCCT ATGGTGATAT GCGCACCCTG 
CACGAACGGG AGAACCTTTC CGGATTGGGC GGTACTGGGC CAGCGCACTA TGCCCAGAAC 
TCGCTGCCAC GCGCCAAATC TGATTTCAAT CTGGCTTTGG TGGGCACTGG ACAGAGCTCA 
GCCAACAAAC ACAAGATCAT TGAGGAGCAG CTAGCCACTC TGGGCGACGA TCAGCGCGGT 
GATCAGCGTC CTCTGGTGAA GGCCCTCTAC GCCTACATGC CCTCCGGCGA GAACCAGTTG 
TCCTTCGAGG AGGGCGACCG CATTGCTTTG GTGGGCGGAA AGGCCAAGGG CTGGCAATTC 
GGTGAGAATC TGCGGACACA GCACTTCGGC TGGTTTCCGG TGGCCTACAC CAATGCGGAA 
GTGGCAGAGG TGACCACTTC CGGTGGTCGG CGATACGAAA ACATGCTGAT GAGCTATGAG 
CGAAATGGTG GCGGATCAGG ATCCGGTTCT GGCGGAGGTG GTCTGCGTAG CTATCAGCAA 
CAGCAGGATT ACGAGGCGCA GCTGGAGCTG ATGGAGAGCC ATGAGGCAAC GTACCGCAGG 
CGAAGGAATC ACAGTGCCGA GGATTCATCA CCCACGCGGA TGTTCGGCGA CACAATCAAG 
CAGCAGAAGA AGTACCGCCC CGGATCCGGG GCCAATCCGC GACCGGGACC GCCACCCACA 
CTGCCCGCTC CGGTGCCCAA TGGACCGCAG AGCCAGTCTT CCAGAATGCT GAACAGCTCG 
CAAAGCTTCT GCGCCTCCAA CGGAGGAGGA GTCAATGCGG CGGTGGAAAG ACGCAAACAA 
AAGCTACTTA ATGGCGTTCA GAGCTCGAAG AACCATCCAT CGGGCAAATC CGGAGTGGCG 
GCCAAGACCT CGCTGCATAG CAGCAATGAC AGTGGATTCG CCAACGAGCC TCCACCACAG 
CCGGAAGTTG ACTATTCGGA TGAGGAGCCA GCCACCCAAA GAGTGGCCAT TCGTCGCGGT 
ATATATCGCA ACTATTCCAT AGATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097583.2
CDS	881..2770
Translation	MEAEEVTKLVDGVYRNILDRFNPGARQLIAAGKSYLKALHGAATASRLFNEALAKIAMNAQQSGTGDIGSALMSVVNVNKEIQDQQMNILKAFYVDLLVPLETNLEKDTKVVQHEQKKFLQQHKVRMESYQKAVSTMKKQRKKKATPENTEKELRSLQLLEDQKKKLDVFCDQSYKNAMTQERRRYGFVLERQCSIAKHWMVYHTTGKTVIDNNFENWQEIAASREIIPPAAYESGYSTSNGHNNNNISSSSSSGNNNTSGNMRRLERIKDGDDDHLQGHGAQLKKSRSIDAPYGDMRTLHERENLSGLGGTGPAHYAQNSLPRAKSDFNLALVGTGQSSANKHKIIEEQLATLGDDQRGDQRPLVKALYAYMPSGENQLSFEEGDRIALVGGKAKGWQFGENLRTQHFGWFPVAYTNAEVAEVTTSGGRRYENMLMSYERNGGGSGSGSGGGGLRSYQQQQDYEAQLELMESHEATYRRRRNHSAEDSSPTRMFGDTIKQQKKYRPGSGANPRPGPPPTLPAPVPNGPQSQSSRMLNSSQSFCASNGGGVNAAVERRKQKLLNGVQSSKNHPSGKSGVAAKTSLHSSNDSGFANEPPPQPEVDYSDEEPATQRVAIRRGIYRNYSIDY

Target ORF information:

RefSeq Version	NM_001104113.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster insulin receptor substrate 53 kDa, transcript variant D (IRSp53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104113.2

ORF Insert Sequence:

ATGGAGGCCG AGGAAGTTAC AAAACTCGTC GATGGCGTGT ATCGGAACAT ACTGGATAGA 
TTCAATCCGG GTGCCAGGCA GCTGATCGCA GCGGGCAAAA GTTACCTCAA GGCGTTGCAC 
GGTGCCGCAA CCGCCTCGCG TCTATTCAAT GAAGCATTGG CCAAGATTGC GATGAACGCC 
CAGCAAAGCG GAACGGGCGA TATTGGTTCA GCTTTGATGA GCGTTGTGAA TGTGAACAAG 
GAGATACAGG ATCAGCAGAT GAATATTCTG AAAGCCTTCT ACGTGGATCT ATTGGTGCCA 
TTGGAGACAA ATCTGGAGAA GGACACAAAG GTGGTGCAGC ATGAGCAAAA GAAGTTCCTG 
CAGCAGCACA AAGTGCGAAT GGAGAGCTAT CAGAAGGCCG TGTCCACGAT GAAGAAGCAG 
CGCAAGAAAA AGGCCACGCC CGAGAACACC GAAAAGGAGC TTAGGAGCCT CCAATTGCTC 
GAGGATCAGA AGAAAAAGCT GGATGTGTTC TGCGATCAGA GCTACAAGAA TGCCATGACA 
CAGGAGCGAC GACGGTACGG ATTCGTCTTG GAGCGCCAGT GCTCGATCGC CAAACACTGG 
ATGGTCTACC ACACCACCGG CAAGACGGTA ATTGATAATA ATTTCGAAAA TTGGCAAGAG 
ATTGCCGCTT CTCGTGAGAT CATTCCACCG GCGGCCTATG AAAGTGGATA CAGTACCAGC 
AACGGACACA ACAACAACAA CATCAGCAGC AGCAGCAGCA GCGGCAACAA CAACACCAGC 
GGCAACATGC GACGCCTGGA ACGAATTAAG GACGGCGACG ATGACCACCT CCAGGGTCAC 
GGAGCTCAGC TGAAGAAATC GCGCAGCATC GATGCCCCCT ATGGTGATAT GCGCACCCTG 
CACGAACGGG AGAACCTTTC CGGATTGGGC GGTACTGGGC CAGCGCACTA TGCCCAGAAC 
TCGCTGCCAC GCGCCAAATC TGATTTCAAT CTGGCTTTGG TGGGCACTGG ACAGAGCTCA 
GCCAACAAAC ACAAGATCAT TGAGGAGCAG CTAGCCACTC TGGGCGACGA TCAGCGCGGT 
GATCAGCGTC CTCTGGTGAA GGCCCTCTAC GCCTACATGC CCTCCGGCGA GAACCAGTTG 
TCCTTCGAGG AGGGCGACCG CATTGCTTTG GTGGGCGGAA AGGCCAAGGG CTGGCAATTC 
GGTGAGAATC TGCGGACACA GCACTTCGGC TGGTTTCCGG TGGCCTACAC CAATGCGGAA 
GTGGCAGAGG TGACCACTTC CGGTGGTCGG CGATACGAAA ACATGCTGAT GAGCTATGAG 
CGAAATGGTG GCGGATCAGG ATCCGGTTCT GGCGGAGGTG GTCTGCGTAG CTATCAGCAA 
CAGCAGGATT ACGAGGCGCA GCTGGAGCTG ATGGAGAGCC ATGAGGCAAC GTACCGCAGG 
CGAAGGAATC ACAGTGCCGA GGATTCATCA CCCACGCGGA TGTTCGGCGA CACAATCAAG 
CAGCAGAAGA AGTACCGCCC CGGATCCGGG GCCAATCCGC GACCGGGACC GCCACCCACA 
CTGCCCGCTC CGGTGCCCAA TGGACCGCAG AGCCAGTCTT CCAGAATGCT GAACAGCTCG 
CAAAGCTTCT GCGCCTCCAA CGGAGGAGGA GTCAATGCGG CGGTGGAAAG ACGCAAACAA 
AAGCTACTTA ATGGCGTTCA GAGCTCGAAG AACCATCCAT CGGGCAAATC CGGAGTGGCG 
GCCAAGACCT CGCTGCATAG CAGCAATGAC AGTGGATTCG CCAACGAGCC TCCACCACAG 
CCGGAAGTTG ACTATTCGGA TGAGGAGCCA GCCACCCAAA GAGTGGCCAT TCGTCGCGGT 
ATATATCGCA ACTATTCCAT AGATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IRSp53 ( NM_001300098.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287027.1 Drosophila melanogaster insulin receptor substrate 53 kDa, transcript variant E (IRSp53), mRNA.

Price & Availability↑

CloneID	OFa22007
Clone ID Related Accession (Same CDS sequence)	NM_001300098.1
Accession Version	NM_001300098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	insulin receptor substrate 53 kDa, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGCCG AGGAAGTTAC AAAACTCGTC GATGGCGTGT ATCGGAACAT ACTGGATAGA 
TTCAATCCGG GTGCCAGGCA GCTGATCGCA GCGGGCAAAA GTTACCTCAA GGCGTTGCAC 
GGTGCCGCAA CCGCCTCGCG TCTATTCAAT GAAGCATTGG CCAAGATTGC GATGAACGCC 
CAGCAAAGCG GAACGGGCGA TATTGGTTCA GCTTTGATGA GCGTTGTGAA TGTGAACAAG 
GAGATACAGG ATCAGCAGAT GAATATTCTG AAAGCCTTCT ACGTGGATCT ATTGGTGCCA 
TTGGAGACAA ATCTGGAGAA GGACACAAAG GTGGTGCAGC ATGAGCAAAA GAAGTTCCTG 
CAGCAGCACA AAGTGCGAAT GGAGAGCTAT CAGAAGGCCG TGTCCACGAT GAAGAAGCAG 
CGCAAGAAAA AGGCCACGCC CGAGAACACC GAAAAGGAGC TTAGGAGCCT CCAATTGCTC 
GAGGATCAGA AGAAAAAGCT GGATGTGTTC TGCGATCAGA GCTACAAGAA TGCCATGACA 
CAGGAGCGAC GACGGTACGG ATTCGTCTTG GAGCGCCAGT GCTCGATCGC CAAACACTGG 
ATGGTCTACC ACACCACCGG CAAGACGGTA ATTGATAATA ATTTCGAAAA TTGGCAAGAG 
ATTGCCGCTT CTCGTGAGAT CATTCCACCG GCGGCCTATG AAAGTGGATA CAGTACCAGC 
AACGGACACA ACAACAACAA CATCAGCAGC AGCAGCAGCA GCGGCAACAA CAACACCAGC 
GGCAACATGC GACGCCTGGA ACGAATTAAG GACGGCGACG ATGACCACCT CCAGGGTCAC 
GGAGCTCAGC TGAAGAAATC GCGCAGCATC GATGCCCCCT ATGGTGATAT GCGCACCCTG 
CACGAACGGG AGAACCTTTC CGGATTGGGC GGTACTGGGC CAGCGCACTA TGCCCAGAAC 
TCGCTGCCAC GCGCCAAATC TGATTTCAAT CTGGCTTTGG TGGGCACTGG ACAGAGCTCA 
GCCAACAAAC ACAAGATCAT TGAGGAGCAG CTAGCCACTC TGGGCGACGA TCAGCGCGGT 
GATCAGCGTC CTCTGGTGAA GGCCCTCTAC GCCTACATGC CCTCCGGCGA GAACCAGTTG 
TCCTTCGAGG AGGGCGACCG CATTGCTTTG GTGGGCGGAA AGGCCAAGGG CTGGCAATTC 
GGTGAGAATC TGCGGACACA GCACTTCGGC TGGTTTCCGG TGGCCTACAC CAATGCGGAA 
GTGGCAGAGG TGACCACTTC CGGTGGTCGG CGATACGAAA ACATGCTGAT GAGCTATGAG 
CGAAATGGTG GCGGATCAGG ATCCGGTTCT GGCGGAGGTG GTCTGCGTAG CTATCAGCAA 
CAGCAGGATT ACGAGGCGCA GCTGGAGCTG ATGGAGAGCC ATGAGGCAAC GTACCGCAGG 
CGAAGGAATC ACAGTGCCGA GGATTCATCA CCCACGCGGA TGTTCGGCGA CACAATCAAG 
CAGCAGAAGA AGTACCGCCC CGGATCCGGG GCCAATCCGC GACCGGGACC GCCACCCACA 
CTGCCCGCTC CGGTGCCCAA TGGACCGCAG AGCCAGTCTT CCAGAATGCT GAACAGCTCG 
CAAAGCTTCT GCGCCTCCAA CGGAGGAGGA GTCAATGCGG CGGTGGAAAG ACGCAAACAA 
AAGCTACTTA ATGGCGTTCA GAGCTCGAAG AACCATCCAT CGGGCAAATC CGGAGTGGCG 
GCCAAGACCT CGCTGCATAG CAGCAATGAC AGTGGATTCG CCAACGAGCC TCCACCACAG 
CCGGAAGTTG ACTATTCGGA TGAGGAGCCA GCCACCCAAA GAGTGGCCAT TCGAGCCTCC 
CAGTTCGCCA CGGTCAAGTT GCGACATACC AAGACCAACG ACCGATCGGC ACCGATGATC 
TATCGGAGTG TGAACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287027.1
CDS	881..2821
Translation	MEAEEVTKLVDGVYRNILDRFNPGARQLIAAGKSYLKALHGAATASRLFNEALAKIAMNAQQSGTGDIGSALMSVVNVNKEIQDQQMNILKAFYVDLLVPLETNLEKDTKVVQHEQKKFLQQHKVRMESYQKAVSTMKKQRKKKATPENTEKELRSLQLLEDQKKKLDVFCDQSYKNAMTQERRRYGFVLERQCSIAKHWMVYHTTGKTVIDNNFENWQEIAASREIIPPAAYESGYSTSNGHNNNNISSSSSSGNNNTSGNMRRLERIKDGDDDHLQGHGAQLKKSRSIDAPYGDMRTLHERENLSGLGGTGPAHYAQNSLPRAKSDFNLALVGTGQSSANKHKIIEEQLATLGDDQRGDQRPLVKALYAYMPSGENQLSFEEGDRIALVGGKAKGWQFGENLRTQHFGWFPVAYTNAEVAEVTTSGGRRYENMLMSYERNGGGSGSGSGGGGLRSYQQQQDYEAQLELMESHEATYRRRRNHSAEDSSPTRMFGDTIKQQKKYRPGSGANPRPGPPPTLPAPVPNGPQSQSSRMLNSSQSFCASNGGGVNAAVERRKQKLLNGVQSSKNHPSGKSGVAAKTSLHSSNDSGFANEPPPQPEVDYSDEEPATQRVAIRASQFATVKLRHTKTNDRSAPMIYRSVNK

Target ORF information:

RefSeq Version	NM_001300098.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster insulin receptor substrate 53 kDa, transcript variant E (IRSp53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300098.1

ORF Insert Sequence:

ATGGAGGCCG AGGAAGTTAC AAAACTCGTC GATGGCGTGT ATCGGAACAT ACTGGATAGA 
TTCAATCCGG GTGCCAGGCA GCTGATCGCA GCGGGCAAAA GTTACCTCAA GGCGTTGCAC 
GGTGCCGCAA CCGCCTCGCG TCTATTCAAT GAAGCATTGG CCAAGATTGC GATGAACGCC 
CAGCAAAGCG GAACGGGCGA TATTGGTTCA GCTTTGATGA GCGTTGTGAA TGTGAACAAG 
GAGATACAGG ATCAGCAGAT GAATATTCTG AAAGCCTTCT ACGTGGATCT ATTGGTGCCA 
TTGGAGACAA ATCTGGAGAA GGACACAAAG GTGGTGCAGC ATGAGCAAAA GAAGTTCCTG 
CAGCAGCACA AAGTGCGAAT GGAGAGCTAT CAGAAGGCCG TGTCCACGAT GAAGAAGCAG 
CGCAAGAAAA AGGCCACGCC CGAGAACACC GAAAAGGAGC TTAGGAGCCT CCAATTGCTC 
GAGGATCAGA AGAAAAAGCT GGATGTGTTC TGCGATCAGA GCTACAAGAA TGCCATGACA 
CAGGAGCGAC GACGGTACGG ATTCGTCTTG GAGCGCCAGT GCTCGATCGC CAAACACTGG 
ATGGTCTACC ACACCACCGG CAAGACGGTA ATTGATAATA ATTTCGAAAA TTGGCAAGAG 
ATTGCCGCTT CTCGTGAGAT CATTCCACCG GCGGCCTATG AAAGTGGATA CAGTACCAGC 
AACGGACACA ACAACAACAA CATCAGCAGC AGCAGCAGCA GCGGCAACAA CAACACCAGC 
GGCAACATGC GACGCCTGGA ACGAATTAAG GACGGCGACG ATGACCACCT CCAGGGTCAC 
GGAGCTCAGC TGAAGAAATC GCGCAGCATC GATGCCCCCT ATGGTGATAT GCGCACCCTG 
CACGAACGGG AGAACCTTTC CGGATTGGGC GGTACTGGGC CAGCGCACTA TGCCCAGAAC 
TCGCTGCCAC GCGCCAAATC TGATTTCAAT CTGGCTTTGG TGGGCACTGG ACAGAGCTCA 
GCCAACAAAC ACAAGATCAT TGAGGAGCAG CTAGCCACTC TGGGCGACGA TCAGCGCGGT 
GATCAGCGTC CTCTGGTGAA GGCCCTCTAC GCCTACATGC CCTCCGGCGA GAACCAGTTG 
TCCTTCGAGG AGGGCGACCG CATTGCTTTG GTGGGCGGAA AGGCCAAGGG CTGGCAATTC 
GGTGAGAATC TGCGGACACA GCACTTCGGC TGGTTTCCGG TGGCCTACAC CAATGCGGAA 
GTGGCAGAGG TGACCACTTC CGGTGGTCGG CGATACGAAA ACATGCTGAT GAGCTATGAG 
CGAAATGGTG GCGGATCAGG ATCCGGTTCT GGCGGAGGTG GTCTGCGTAG CTATCAGCAA 
CAGCAGGATT ACGAGGCGCA GCTGGAGCTG ATGGAGAGCC ATGAGGCAAC GTACCGCAGG 
CGAAGGAATC ACAGTGCCGA GGATTCATCA CCCACGCGGA TGTTCGGCGA CACAATCAAG 
CAGCAGAAGA AGTACCGCCC CGGATCCGGG GCCAATCCGC GACCGGGACC GCCACCCACA 
CTGCCCGCTC CGGTGCCCAA TGGACCGCAG AGCCAGTCTT CCAGAATGCT GAACAGCTCG 
CAAAGCTTCT GCGCCTCCAA CGGAGGAGGA GTCAATGCGG CGGTGGAAAG ACGCAAACAA 
AAGCTACTTA ATGGCGTTCA GAGCTCGAAG AACCATCCAT CGGGCAAATC CGGAGTGGCG 
GCCAAGACCT CGCTGCATAG CAGCAATGAC AGTGGATTCG CCAACGAGCC TCCACCACAG 
CCGGAAGTTG ACTATTCGGA TGAGGAGCCA GCCACCCAAA GAGTGGCCAT TCGAGCCTCC 
CAGTTCGCCA CGGTCAAGTT GCGACATACC AAGACCAACG ACCGATCGGC ACCGATGATC 
TATCGGAGTG TGAACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IRSp53 ( NM_168448.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729679.2 Drosophila melanogaster insulin receptor substrate 53 kDa, transcript variant A (IRSp53), mRNA.

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CloneID	OFa18220
Clone ID Related Accession (Same CDS sequence)	NM_168448.3
Accession Version	NM_168448.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	insulin receptor substrate 53 kDa, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168448.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGCCG AGGAAGTTAC AAAACTCGTC GATGGCGTGT ATCGGAACAT ACTGGATAGA 
TTCAATCCGG GTGCCAGGCA GCTGATCGCA GCGGGCAAAA GTTACCTCAA GGCGTTGCAC 
GGTGCCGCAA CCGCCTCGCG TCTATTCAAT GAAGCATTGG CCAAGATTGC GATGAACGCC 
CAGCAAAGCG GAACGGGCGA TATTGGTTCA GCTTTGATGA GCGTTGTGAA TGTGAACAAG 
GAGATACAGG ATCAGCAGAT GAATATTCTG AAAGCCTTCT ACGTGGATCT ATTGGTGCCA 
TTGGAGACAA ATCTGGAGAA GGACACAAAG GTGGTGCAGC ATGAGCAAAA GAAGTTCCTG 
CAGCAGCACA AAGTGCGAAT GGAGAGCTAT CAGAAGGCCG TGTCCACGAT GAAGAAGCAG 
CGCAAGAAAA AGGCCACGCC CGAGAACACC GAAAAGGAGC TTAGGAGCCT CCAATTGCTC 
GAGGATCAGA AGAAAAAGCT GGATGTGTTC TGCGATCAGA GCTACAAGAA TGCCATGACA 
CAGGAGCGAC GACGGTACGG ATTCGTCTTG GAGCGCCAGT GCTCGATCGC CAAACACTGG 
ATGGTCTACC ACACCACCGG CAAGACGGTA ATTGATAATA ATTTCGAAAA TTGGCAAGAG 
ATTGCCGCTT CTCGTGAGAT CATTCCACCG GCGGCCTATG AAAGTGGATA CAGTACCAGC 
AACGGACACA ACAACAACAA CATCAGCAGC AGCAGCAGCA GCGGCAACAA CAACACCAGC 
GGCAACATGC GACGCCTGGA ACGAATTAAG GACGGCGACG ATGACCACCT CCAGGGTCAC 
GGAGCTCAGC TGAAGAAATC GCGCAGCATC GATGCCCCCT ATGGTGATAT GCGCACCCTG 
CACGAACGGG AGAACCTTTC CGGATTGGGC GGTACTGGGC CAGCGCACTA TGCCCAGAAC 
TCGCTGCCAC GCGCCAAATC TGATTTCAAT CTGGCTTTGG TGGGCACTGG ACAGAGCTCA 
GCCAACAAAC ACAAGATCAT TGAGGAGCAG CTAGCCACTC TGGGCGACGA TCAGCGCGGT 
GATCAGCGTC CTCTGGTGAA GGCCCTCTAC GCCTACATGC CCTCCGGCGA GAACCAGTTG 
TCCTTCGAGG AGGGCGACCG CATTGCTTTG GTGGGCGGAA AGGCCAAGGG CTGGCAATTC 
GGTGAGAATC TGCGGACACA GCACTTCGGC TGGTTTCCGG TGGCCTACAC CAATGCGGAA 
GTGGCAGAGG TGACCACTTC CGGTGGTCGG CGATACGAAA ACATGCTGAT GAGCTATGAG 
CGAAATGGTG GCGGATCAGG ATCCGGTTCT GGCGGAGGTG GTCTGCGTAG CTATCAGCAA 
CAGCAGGATT ACGAGGCGCA GCTGGAGCTG ATGGAGAGCC ATGAGGCAAC GTACCGCAGG 
CGAAGGAATC ACAGTGCCGA GGATTCATCA CCCACGCGGA TGTTCGGCGA CACAATCAAG 
CAGCAGAAGA AGTACCGCCC CGGATCCGGG GCCAATCCGC GACCGGGACC GCCACCCACA 
CTGCCCGCTC CGGTGCCCAA TGGACCGCAG AGCCAGTCTT CCAGAATGCT GAACAGCTCG 
CAAAGCTTCT GCGCCTCCAA CGGAGGAGGA GTCAATGCGG CGGTGGAAAG ACGCAAACAA 
AAGCTACTTA ATGGCGTTCA GAGCTCGAAG AACCATCCAT CGGGCAAATC CGGAGTGGCG 
GCCAAGACCT CGCTGCATAG CAGCAATGAC AGTGGATTCG CCAACGAGCC TCCACCACAG 
CCGGAAGTTG ACTATTCGGA TGAGGAGCCA GCCACCCAAA GAGTGGCCAT TCGACGCCGG 
GCGGACACCA ATTCGCATAC CAACACGGTG CCGAGGGATG TGGCCTCGTG GACGTTGAAC 
CGGAACTTCC GGAATAGCGT CGATAGTCAG ATCGACGACA AGAGCCTGCA TCGGCTGAGC 
CGGCAGAGCG GAGGAGTGGG AAAGATGCGA TACGATCTTA TTGCCTCCGA TGACGAGATT 
CTTCAGGCTT CCAGCGCCGG TCTGAAGCGC ACCAAATCGT TCTGGAAATT TGGCGGCGGA 
CGCAATGGCG AAGATATACT GGCCGGCATG TCATTGTGGC AACACCGCGA CCTGGTGGCG 
GCTCCAAATC TGGAGGAGAT GCGCGATCGG GATGACTTGG AGCGGCGTTT GGAGGAGGAT 
CGCAATGGTA CACTGACCAA GTCGCATAAC TCCAGTTCCT CGCAGGAGAA GCGCGAACGA 
AAGGACAGCC TGACCAGCAC TGAATTGTAT GGAGATGAGG ATGAGAATAT CTACGGAGTG 
AGTCCACAGC AACCGCCACT GCAGCAGCAG ACGCAGCAGC ATCAGCAACA TCAGCAACAT 
CAGCAGCAAC AGGCGCAGCA GCACAGGAGC AGCTATACTC CCAAATCCCG CATGAAAATC 
ATGAAGACCA TTGAGATTGA CATTGAGGAG CCAACGGAGG CGGAGCGTAT GAGCACCATG 
CGACGTGGTC AGTCGCAGGA TCAGCAGCAG CAACATCGAA AGTTAGACAA ACAGGTTTCC 
GGCACCGGAT CCCAGCACAA ATCCCTGACT TTGAGTCGCA CCAAAAGCAA CCAGCAGCAG 
CAGCAGCAGC AGCAGTTTCC ACAATCCACG GATGAGGGTG AAAGCGATGA GCACGGTACC 
CTGAAGCTCA GCGATGTGAA CAACTTCTTT GACGGCGAGG GCAAGAACAA CAGTCACGGC 
CATGGACTGG TCATGAAGAC GGTGAAGCGA CAGGACATAC TCAAGCAGTA TTATACCAGC 
GAGGACGAGG AGGATTCCGA TGCGGAACTC AAGTCGACTA GTTCCGATCC CTACGATTGC 
ATCGTAATCA ATGATCACCT GGTGCGCAAG GACGACAAGA TGCGGCGCCA TCACCAGCAG 
CAGCATCAGC AAATGGCGAC CAATACGCTG AAAAAGAGCT CCTCCAAGGA CCAGGACAGC 
ACACTGACCC GCAATTCGAC GGCCAGTTCG GGAGCTCCGA CGGCTACCAT TCTGCCGCGA 
ACACGCCTGC TCAAGTCCTC CACCTCCGCT GGCAATAACA TCGGCAACAG CAGCGCCACC 
TTGGAGCGGA ACTCAAAGGG TTTGGCCGGA AACAAGAGCT ACGGACCATG GTACGACTTC 
TGGGATCAGG AACAGCATGG CCAGAAGTCG GCTAGTGCCA AGCTGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729679.2
CDS	881..4111
Translation	MEAEEVTKLVDGVYRNILDRFNPGARQLIAAGKSYLKALHGAATASRLFNEALAKIAMNAQQSGTGDIGSALMSVVNVNKEIQDQQMNILKAFYVDLLVPLETNLEKDTKVVQHEQKKFLQQHKVRMESYQKAVSTMKKQRKKKATPENTEKELRSLQLLEDQKKKLDVFCDQSYKNAMTQERRRYGFVLERQCSIAKHWMVYHTTGKTVIDNNFENWQEIAASREIIPPAAYESGYSTSNGHNNNNISSSSSSGNNNTSGNMRRLERIKDGDDDHLQGHGAQLKKSRSIDAPYGDMRTLHERENLSGLGGTGPAHYAQNSLPRAKSDFNLALVGTGQSSANKHKIIEEQLATLGDDQRGDQRPLVKALYAYMPSGENQLSFEEGDRIALVGGKAKGWQFGENLRTQHFGWFPVAYTNAEVAEVTTSGGRRYENMLMSYERNGGGSGSGSGGGGLRSYQQQQDYEAQLELMESHEATYRRRRNHSAEDSSPTRMFGDTIKQQKKYRPGSGANPRPGPPPTLPAPVPNGPQSQSSRMLNSSQSFCASNGGGVNAAVERRKQKLLNGVQSSKNHPSGKSGVAAKTSLHSSNDSGFANEPPPQPEVDYSDEEPATQRVAIRRRADTNSHTNTVPRDVASWTLNRNFRNSVDSQIDDKSLHRLSRQSGGVGKMRYDLIASDDEILQASSAGLKRTKSFWKFGGGRNGEDILAGMSLWQHRDLVAAPNLEEMRDRDDLERRLEEDRNGTLTKSHNSSSSQEKRERKDSLTSTELYGDEDENIYGVSPQQPPLQQQTQQHQQHQQHQQQQAQQHRSSYTPKSRMKIMKTIEIDIEEPTEAERMSTMRRGQSQDQQQQHRKLDKQVSGTGSQHKSLTLSRTKSNQQQQQQQQFPQSTDEGESDEHGTLKLSDVNNFFDGEGKNNSHGHGLVMKTVKRQDILKQYYTSEDEEDSDAELKSTSSDPYDCIVINDHLVRKDDKMRRHHQQQHQQMATNTLKKSSSKDQDSTLTRNSTASSGAPTATILPRTRLLKSSTSAGNNIGNSSATLERNSKGLAGNKSYGPWYDFWDQEQHGQKSASAKLK

Target ORF information:

RefSeq Version	NM_168448.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster insulin receptor substrate 53 kDa, transcript variant A (IRSp53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168448.3

ORF Insert Sequence:

ATGGAGGCCG AGGAAGTTAC AAAACTCGTC GATGGCGTGT ATCGGAACAT ACTGGATAGA 
TTCAATCCGG GTGCCAGGCA GCTGATCGCA GCGGGCAAAA GTTACCTCAA GGCGTTGCAC 
GGTGCCGCAA CCGCCTCGCG TCTATTCAAT GAAGCATTGG CCAAGATTGC GATGAACGCC 
CAGCAAAGCG GAACGGGCGA TATTGGTTCA GCTTTGATGA GCGTTGTGAA TGTGAACAAG 
GAGATACAGG ATCAGCAGAT GAATATTCTG AAAGCCTTCT ACGTGGATCT ATTGGTGCCA 
TTGGAGACAA ATCTGGAGAA GGACACAAAG GTGGTGCAGC ATGAGCAAAA GAAGTTCCTG 
CAGCAGCACA AAGTGCGAAT GGAGAGCTAT CAGAAGGCCG TGTCCACGAT GAAGAAGCAG 
CGCAAGAAAA AGGCCACGCC CGAGAACACC GAAAAGGAGC TTAGGAGCCT CCAATTGCTC 
GAGGATCAGA AGAAAAAGCT GGATGTGTTC TGCGATCAGA GCTACAAGAA TGCCATGACA 
CAGGAGCGAC GACGGTACGG ATTCGTCTTG GAGCGCCAGT GCTCGATCGC CAAACACTGG 
ATGGTCTACC ACACCACCGG CAAGACGGTA ATTGATAATA ATTTCGAAAA TTGGCAAGAG 
ATTGCCGCTT CTCGTGAGAT CATTCCACCG GCGGCCTATG AAAGTGGATA CAGTACCAGC 
AACGGACACA ACAACAACAA CATCAGCAGC AGCAGCAGCA GCGGCAACAA CAACACCAGC 
GGCAACATGC GACGCCTGGA ACGAATTAAG GACGGCGACG ATGACCACCT CCAGGGTCAC 
GGAGCTCAGC TGAAGAAATC GCGCAGCATC GATGCCCCCT ATGGTGATAT GCGCACCCTG 
CACGAACGGG AGAACCTTTC CGGATTGGGC GGTACTGGGC CAGCGCACTA TGCCCAGAAC 
TCGCTGCCAC GCGCCAAATC TGATTTCAAT CTGGCTTTGG TGGGCACTGG ACAGAGCTCA 
GCCAACAAAC ACAAGATCAT TGAGGAGCAG CTAGCCACTC TGGGCGACGA TCAGCGCGGT 
GATCAGCGTC CTCTGGTGAA GGCCCTCTAC GCCTACATGC CCTCCGGCGA GAACCAGTTG 
TCCTTCGAGG AGGGCGACCG CATTGCTTTG GTGGGCGGAA AGGCCAAGGG CTGGCAATTC 
GGTGAGAATC TGCGGACACA GCACTTCGGC TGGTTTCCGG TGGCCTACAC CAATGCGGAA 
GTGGCAGAGG TGACCACTTC CGGTGGTCGG CGATACGAAA ACATGCTGAT GAGCTATGAG 
CGAAATGGTG GCGGATCAGG ATCCGGTTCT GGCGGAGGTG GTCTGCGTAG CTATCAGCAA 
CAGCAGGATT ACGAGGCGCA GCTGGAGCTG ATGGAGAGCC ATGAGGCAAC GTACCGCAGG 
CGAAGGAATC ACAGTGCCGA GGATTCATCA CCCACGCGGA TGTTCGGCGA CACAATCAAG 
CAGCAGAAGA AGTACCGCCC CGGATCCGGG GCCAATCCGC GACCGGGACC GCCACCCACA 
CTGCCCGCTC CGGTGCCCAA TGGACCGCAG AGCCAGTCTT CCAGAATGCT GAACAGCTCG 
CAAAGCTTCT GCGCCTCCAA CGGAGGAGGA GTCAATGCGG CGGTGGAAAG ACGCAAACAA 
AAGCTACTTA ATGGCGTTCA GAGCTCGAAG AACCATCCAT CGGGCAAATC CGGAGTGGCG 
GCCAAGACCT CGCTGCATAG CAGCAATGAC AGTGGATTCG CCAACGAGCC TCCACCACAG 
CCGGAAGTTG ACTATTCGGA TGAGGAGCCA GCCACCCAAA GAGTGGCCAT TCGACGCCGG 
GCGGACACCA ATTCGCATAC CAACACGGTG CCGAGGGATG TGGCCTCGTG GACGTTGAAC 
CGGAACTTCC GGAATAGCGT CGATAGTCAG ATCGACGACA AGAGCCTGCA TCGGCTGAGC 
CGGCAGAGCG GAGGAGTGGG AAAGATGCGA TACGATCTTA TTGCCTCCGA TGACGAGATT 
CTTCAGGCTT CCAGCGCCGG TCTGAAGCGC ACCAAATCGT TCTGGAAATT TGGCGGCGGA 
CGCAATGGCG AAGATATACT GGCCGGCATG TCATTGTGGC AACACCGCGA CCTGGTGGCG 
GCTCCAAATC TGGAGGAGAT GCGCGATCGG GATGACTTGG AGCGGCGTTT GGAGGAGGAT 
CGCAATGGTA CACTGACCAA GTCGCATAAC TCCAGTTCCT CGCAGGAGAA GCGCGAACGA 
AAGGACAGCC TGACCAGCAC TGAATTGTAT GGAGATGAGG ATGAGAATAT CTACGGAGTG 
AGTCCACAGC AACCGCCACT GCAGCAGCAG ACGCAGCAGC ATCAGCAACA TCAGCAACAT 
CAGCAGCAAC AGGCGCAGCA GCACAGGAGC AGCTATACTC CCAAATCCCG CATGAAAATC 
ATGAAGACCA TTGAGATTGA CATTGAGGAG CCAACGGAGG CGGAGCGTAT GAGCACCATG 
CGACGTGGTC AGTCGCAGGA TCAGCAGCAG CAACATCGAA AGTTAGACAA ACAGGTTTCC 
GGCACCGGAT CCCAGCACAA ATCCCTGACT TTGAGTCGCA CCAAAAGCAA CCAGCAGCAG 
CAGCAGCAGC AGCAGTTTCC ACAATCCACG GATGAGGGTG AAAGCGATGA GCACGGTACC 
CTGAAGCTCA GCGATGTGAA CAACTTCTTT GACGGCGAGG GCAAGAACAA CAGTCACGGC 
CATGGACTGG TCATGAAGAC GGTGAAGCGA CAGGACATAC TCAAGCAGTA TTATACCAGC 
GAGGACGAGG AGGATTCCGA TGCGGAACTC AAGTCGACTA GTTCCGATCC CTACGATTGC 
ATCGTAATCA ATGATCACCT GGTGCGCAAG GACGACAAGA TGCGGCGCCA TCACCAGCAG 
CAGCATCAGC AAATGGCGAC CAATACGCTG AAAAAGAGCT CCTCCAAGGA CCAGGACAGC 
ACACTGACCC GCAATTCGAC GGCCAGTTCG GGAGCTCCGA CGGCTACCAT TCTGCCGCGA 
ACACGCCTGC TCAAGTCCTC CACCTCCGCT GGCAATAACA TCGGCAACAG CAGCGCCACC 
TTGGAGCGGA ACTCAAAGGG TTTGGCCGGA AACAAGAGCT ACGGACCATG GTACGACTTC 
TGGGATCAGG AACAGCATGG CCAGAAGTCG GCTAGTGCCA AGCTGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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IRSp53 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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