phol cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	phol
Entrez Gene ID	39095
Full Name	pleiohomeotic like
Synonyms	CG3445,Dmel\CG3445,PHOL,Phol
General protein information	Preferred Names pleiohomeotic like Names CG3445 gene product from transcript CG3445-RB CG3445-PA CG3445-PB Pleiohomeotic-like pho-like phol-PA phol-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67B7-67B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_140060.3	NP_648317.1	pleiohomeotic like, isoform A
NM_001300076.1	NP_001287005.1	pleiohomeotic like, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that Pho and Pho-like, a gene encoding a protein with high similarity to Pho, act redundantly to repress homeotic genes.
Title: The Drosophila pho-like gene encodes a YY1-related DNA binding protein that is redundant with pleiohomeotic in homeotic gene silencing.

The following phol gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phol cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18043	NM_001300076.1 Latest version!	Drosophila melanogaster pleiohomeotic like (phol), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa18043	NM_140060.3 Latest version!	Drosophila melanogaster pleiohomeotic like (phol), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

phol ( NM_140060.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648317.1 Drosophila melanogaster pleiohomeotic like (phol), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18043
Clone ID Related Accession (Same CDS sequence)	NM_140060.3 , NM_001300076.1
Accession Version	NM_140060.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pleiohomeotic like, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140060.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAAGTC ATCATCATCA CGTCGGCGTC GGAGGGAACT TCCAGCCTCA ACTGCATCAG 
ATACGAGCGC CCATCGTGAA GAATCTATCG CTGGCCGCTG CGGCGGTACC GTTGCCCCTG 
CACTCCGCCC CGTCGTCGGC ATACTTCACT CATGCACACC AGCCTACGGC GGCTACCATA 
GTCGAGCAAG ATCCGTACAC CGTTTTGGCG GGCGATCCGA TGGGCGAGGA AAGCGAGAGC 
TTCCTGATCG AGGAGATCAT CGACGACTAT GATGATGAGG CTAATATCGT CGTAGACTCA 
GGCGAATTCT TTGAGCACTA TGAATACTAT CCGGTGGATC GGCTGGACAG ACTTACCTCG 
GTCACGGTGG CTACGGCAGA TGCACTGCCG CCGCCGATCA TCATGCAGCA GACCAGCGAC 
GATGAGGAAT TCATCGAGGA GGATGGACAG CAGATGACAT CCTCCTGCGA TGGCGATGGC 
GATGAGGATG ATGAGGCTAC CGCGCCGGCT ACGACAAATG CCTTCGAGAT TGCCTCCGAG 
ATGCTAACCA CCATCGAGGC CACAAACATC AAGGAGGAGC AGCTCGAGAG CGACTTCTAT 
ATGAAGGCGA CCGAGGATTC AAACAGTTCG AATGGCCAGC TGCAGGATTT TAAGCTATTT 
GGAAGCAGTC GCGTCGAGAG GCCCATGGGA AATTCAGCGG CCAAGCGCTG GAAGCAAACA 
AAGGTACCCA TACGCATAAC GCAGGATGAG TTCAATGTCA CCCTTTGGTC CTCGCTCAAC 
TCGGAGGATG AGGACGAGGA GGAGGAGTTG GAGGAACCAC CTGGTTGTGC TGGCACCACA 
TCCGTGACGT CCTCCTCCGC CGCCCAGAAT GAAGGATCAC CCAGCATGCC AAAGCTTATT 
ATTGACGAGC ACATGATCAA CCACGATGTT TTAAGCGATG GCATCGGCAA CGAGTACGTC 
ATCCTACCCG ATCCATTCAG CGCCTCTGCG GTCACGGATG TGGAGGAGGT GGTTAATGCA 
TTTATGGGCG ATGTCAAAGG CGAGGAGGAC AGCCAGCCGC TAAGTGAACA GTTGATCTAT 
GCGGAAAACC AGTTGGAAGA AGCTTATGGT AACATCGAGC TTATCGAGAA CATGCCGAAT 
AATGTGGAGC TGATACAGAC ACCGGGACCA GGTCCAGCCA CTGTGTCGGT AGCGCTATCG 
AAGGTCAACG GATGTGTACC GAATCCACAG CCGGCTCTCC CAAAACTAGT GCTCCAGAAC 
AGCAGCACCA ATGTGCGACT GAAAAGTGCG GGAAATCAGT CAAAGGTGAC AAAGAGGCAA 
CTCACGGCGG CGGCTCAGGT GGCGACACCA AATCCCCCAC CGCCACTAGT GGCTTCCAGC 
AATCCAGCCC GACCCCAACG ACCCACAATT GTAACCAAAT TACCGACTGC AGTGGCCAAT 
CAGACGGCTG GTAATGCCAT GGGAGCAGCT GGCGCGGCCG GCGAAGAGGA TGAGGAGCCA 
AAGTTTCGCT GCACCCATCG CGGCTGCAAC AAGGAGTTTC GCAACCACTC GGCCATGCGC 
AAGCACATGC ACACGCACGG ACCGCGCGGT CATGTGTGCA ACGTCTGCGG CAAGAGCTTT 
GTAGAGAGCT CCAAGCTAAA GCGCCACCAG CTGGTGCACA CCGGCGAGAA GCCCTTTGAG 
TGCACCTTTG AGGGGTGCGG CAAGCGCTTC TCGCTGGACT TCAATCTGCG CACCCACGTA 
CGGATACACA CTGGCGATAG GCCCTACCAC TGTCCCATTG ATGGCTGCTC CAAGTGCTTC 
GCCCAGTCAA CGAACCTCAA GTCCCACATG CTGACGCACA CGAAGCCCAA AAGGAAGTGG 
CCGCGCGCAC CGCAAGTGAG CAACAACAAG ACACCGCTGG TGGCCAGATA CGGGCGACTG 
GAGTTTGGCG AGGATCCTCG TCTAGTGTAC GTAGAACAGA ACGAGGAGGT GGCGTCGGTG 
CTGCTGGATG GCACGTCCCT AACATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648317.1
CDS	648..2657
Translation	MTSHHHHVGVGGNFQPQLHQIRAPIVKNLSLAAAAVPLPLHSAPSSAYFTHAHQPTAATIVEQDPYTVLAGDPMGEESESFLIEEIIDDYDDEANIVVDSGEFFEHYEYYPVDRLDRLTSVTVATADALPPPIIMQQTSDDEEFIEEDGQQMTSSCDGDGDEDDEATAPATTNAFEIASEMLTTIEATNIKEEQLESDFYMKATEDSNSSNGQLQDFKLFGSSRVERPMGNSAAKRWKQTKVPIRITQDEFNVTLWSSLNSEDEDEEEELEEPPGCAGTTSVTSSSAAQNEGSPSMPKLIIDEHMINHDVLSDGIGNEYVILPDPFSASAVTDVEEVVNAFMGDVKGEEDSQPLSEQLIYAENQLEEAYGNIELIENMPNNVELIQTPGPGPATVSVALSKVNGCVPNPQPALPKLVLQNSSTNVRLKSAGNQSKVTKRQLTAAAQVATPNPPPPLVASSNPARPQRPTIVTKLPTAVANQTAGNAMGAAGAAGEEDEEPKFRCTHRGCNKEFRNHSAMRKHMHTHGPRGHVCNVCGKSFVESSKLKRHQLVHTGEKPFECTFEGCGKRFSLDFNLRTHVRIHTGDRPYHCPIDGCSKCFAQSTNLKSHMLTHTKPKRKWPRAPQVSNNKTPLVARYGRLEFGEDPRLVYVEQNEEVASVLLDGTSLTS

Target ORF information:

RefSeq Version	NM_140060.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pleiohomeotic like (phol), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140060.3

ORF Insert Sequence:

ATGACAAGTC ATCATCATCA CGTCGGCGTC GGAGGGAACT TCCAGCCTCA ACTGCATCAG 
ATACGAGCGC CCATCGTGAA GAATCTATCG CTGGCCGCTG CGGCGGTACC GTTGCCCCTG 
CACTCCGCCC CGTCGTCGGC ATACTTCACT CATGCACACC AGCCTACGGC GGCTACCATA 
GTCGAGCAAG ATCCGTACAC CGTTTTGGCG GGCGATCCGA TGGGCGAGGA AAGCGAGAGC 
TTCCTGATCG AGGAGATCAT CGACGACTAT GATGATGAGG CTAATATCGT CGTAGACTCA 
GGCGAATTCT TTGAGCACTA TGAATACTAT CCGGTGGATC GGCTGGACAG ACTTACCTCG 
GTCACGGTGG CTACGGCAGA TGCACTGCCG CCGCCGATCA TCATGCAGCA GACCAGCGAC 
GATGAGGAAT TCATCGAGGA GGATGGACAG CAGATGACAT CCTCCTGCGA TGGCGATGGC 
GATGAGGATG ATGAGGCTAC CGCGCCGGCT ACGACAAATG CCTTCGAGAT TGCCTCCGAG 
ATGCTAACCA CCATCGAGGC CACAAACATC AAGGAGGAGC AGCTCGAGAG CGACTTCTAT 
ATGAAGGCGA CCGAGGATTC AAACAGTTCG AATGGCCAGC TGCAGGATTT TAAGCTATTT 
GGAAGCAGTC GCGTCGAGAG GCCCATGGGA AATTCAGCGG CCAAGCGCTG GAAGCAAACA 
AAGGTACCCA TACGCATAAC GCAGGATGAG TTCAATGTCA CCCTTTGGTC CTCGCTCAAC 
TCGGAGGATG AGGACGAGGA GGAGGAGTTG GAGGAACCAC CTGGTTGTGC TGGCACCACA 
TCCGTGACGT CCTCCTCCGC CGCCCAGAAT GAAGGATCAC CCAGCATGCC AAAGCTTATT 
ATTGACGAGC ACATGATCAA CCACGATGTT TTAAGCGATG GCATCGGCAA CGAGTACGTC 
ATCCTACCCG ATCCATTCAG CGCCTCTGCG GTCACGGATG TGGAGGAGGT GGTTAATGCA 
TTTATGGGCG ATGTCAAAGG CGAGGAGGAC AGCCAGCCGC TAAGTGAACA GTTGATCTAT 
GCGGAAAACC AGTTGGAAGA AGCTTATGGT AACATCGAGC TTATCGAGAA CATGCCGAAT 
AATGTGGAGC TGATACAGAC ACCGGGACCA GGTCCAGCCA CTGTGTCGGT AGCGCTATCG 
AAGGTCAACG GATGTGTACC GAATCCACAG CCGGCTCTCC CAAAACTAGT GCTCCAGAAC 
AGCAGCACCA ATGTGCGACT GAAAAGTGCG GGAAATCAGT CAAAGGTGAC AAAGAGGCAA 
CTCACGGCGG CGGCTCAGGT GGCGACACCA AATCCCCCAC CGCCACTAGT GGCTTCCAGC 
AATCCAGCCC GACCCCAACG ACCCACAATT GTAACCAAAT TACCGACTGC AGTGGCCAAT 
CAGACGGCTG GTAATGCCAT GGGAGCAGCT GGCGCGGCCG GCGAAGAGGA TGAGGAGCCA 
AAGTTTCGCT GCACCCATCG CGGCTGCAAC AAGGAGTTTC GCAACCACTC GGCCATGCGC 
AAGCACATGC ACACGCACGG ACCGCGCGGT CATGTGTGCA ACGTCTGCGG CAAGAGCTTT 
GTAGAGAGCT CCAAGCTAAA GCGCCACCAG CTGGTGCACA CCGGCGAGAA GCCCTTTGAG 
TGCACCTTTG AGGGGTGCGG CAAGCGCTTC TCGCTGGACT TCAATCTGCG CACCCACGTA 
CGGATACACA CTGGCGATAG GCCCTACCAC TGTCCCATTG ATGGCTGCTC CAAGTGCTTC 
GCCCAGTCAA CGAACCTCAA GTCCCACATG CTGACGCACA CGAAGCCCAA AAGGAAGTGG 
CCGCGCGCAC CGCAAGTGAG CAACAACAAG ACACCGCTGG TGGCCAGATA CGGGCGACTG 
GAGTTTGGCG AGGATCCTCG TCTAGTGTAC GTAGAACAGA ACGAGGAGGT GGCGTCGGTG 
CTGCTGGATG GCACGTCCCT AACATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phol ( NM_001300076.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287005.1 Drosophila melanogaster pleiohomeotic like (phol), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18043
Clone ID Related Accession (Same CDS sequence)	NM_140060.3 , NM_001300076.1
Accession Version	NM_001300076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pleiohomeotic like, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAAGTC ATCATCATCA CGTCGGCGTC GGAGGGAACT TCCAGCCTCA ACTGCATCAG 
ATACGAGCGC CCATCGTGAA GAATCTATCG CTGGCCGCTG CGGCGGTACC GTTGCCCCTG 
CACTCCGCCC CGTCGTCGGC ATACTTCACT CATGCACACC AGCCTACGGC GGCTACCATA 
GTCGAGCAAG ATCCGTACAC CGTTTTGGCG GGCGATCCGA TGGGCGAGGA AAGCGAGAGC 
TTCCTGATCG AGGAGATCAT CGACGACTAT GATGATGAGG CTAATATCGT CGTAGACTCA 
GGCGAATTCT TTGAGCACTA TGAATACTAT CCGGTGGATC GGCTGGACAG ACTTACCTCG 
GTCACGGTGG CTACGGCAGA TGCACTGCCG CCGCCGATCA TCATGCAGCA GACCAGCGAC 
GATGAGGAAT TCATCGAGGA GGATGGACAG CAGATGACAT CCTCCTGCGA TGGCGATGGC 
GATGAGGATG ATGAGGCTAC CGCGCCGGCT ACGACAAATG CCTTCGAGAT TGCCTCCGAG 
ATGCTAACCA CCATCGAGGC CACAAACATC AAGGAGGAGC AGCTCGAGAG CGACTTCTAT 
ATGAAGGCGA CCGAGGATTC AAACAGTTCG AATGGCCAGC TGCAGGATTT TAAGCTATTT 
GGAAGCAGTC GCGTCGAGAG GCCCATGGGA AATTCAGCGG CCAAGCGCTG GAAGCAAACA 
AAGGTACCCA TACGCATAAC GCAGGATGAG TTCAATGTCA CCCTTTGGTC CTCGCTCAAC 
TCGGAGGATG AGGACGAGGA GGAGGAGTTG GAGGAACCAC CTGGTTGTGC TGGCACCACA 
TCCGTGACGT CCTCCTCCGC CGCCCAGAAT GAAGGATCAC CCAGCATGCC AAAGCTTATT 
ATTGACGAGC ACATGATCAA CCACGATGTT TTAAGCGATG GCATCGGCAA CGAGTACGTC 
ATCCTACCCG ATCCATTCAG CGCCTCTGCG GTCACGGATG TGGAGGAGGT GGTTAATGCA 
TTTATGGGCG ATGTCAAAGG CGAGGAGGAC AGCCAGCCGC TAAGTGAACA GTTGATCTAT 
GCGGAAAACC AGTTGGAAGA AGCTTATGGT AACATCGAGC TTATCGAGAA CATGCCGAAT 
AATGTGGAGC TGATACAGAC ACCGGGACCA GGTCCAGCCA CTGTGTCGGT AGCGCTATCG 
AAGGTCAACG GATGTGTACC GAATCCACAG CCGGCTCTCC CAAAACTAGT GCTCCAGAAC 
AGCAGCACCA ATGTGCGACT GAAAAGTGCG GGAAATCAGT CAAAGGTGAC AAAGAGGCAA 
CTCACGGCGG CGGCTCAGGT GGCGACACCA AATCCCCCAC CGCCACTAGT GGCTTCCAGC 
AATCCAGCCC GACCCCAACG ACCCACAATT GTAACCAAAT TACCGACTGC AGTGGCCAAT 
CAGACGGCTG GTAATGCCAT GGGAGCAGCT GGCGCGGCCG GCGAAGAGGA TGAGGAGCCA 
AAGTTTCGCT GCACCCATCG CGGCTGCAAC AAGGAGTTTC GCAACCACTC GGCCATGCGC 
AAGCACATGC ACACGCACGG ACCGCGCGGT CATGTGTGCA ACGTCTGCGG CAAGAGCTTT 
GTAGAGAGCT CCAAGCTAAA GCGCCACCAG CTGGTGCACA CCGGCGAGAA GCCCTTTGAG 
TGCACCTTTG AGGGGTGCGG CAAGCGCTTC TCGCTGGACT TCAATCTGCG CACCCACGTA 
CGGATACACA CTGGCGATAG GCCCTACCAC TGTCCCATTG ATGGCTGCTC CAAGTGCTTC 
GCCCAGTCAA CGAACCTCAA GTCCCACATG CTGACGCACA CGAAGCCCAA AAGGAAGTGG 
CCGCGCGCAC CGCAAGTGAG CAACAACAAG ACACCGCTGG TGGCCAGATA CGGGCGACTG 
GAGTTTGGCG AGGATCCTCG TCTAGTGTAC GTAGAACAGA ACGAGGAGGT GGCGTCGGTG 
CTGCTGGATG GCACGTCCCT AACATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287005.1
CDS	648..2657
Translation	MTSHHHHVGVGGNFQPQLHQIRAPIVKNLSLAAAAVPLPLHSAPSSAYFTHAHQPTAATIVEQDPYTVLAGDPMGEESESFLIEEIIDDYDDEANIVVDSGEFFEHYEYYPVDRLDRLTSVTVATADALPPPIIMQQTSDDEEFIEEDGQQMTSSCDGDGDEDDEATAPATTNAFEIASEMLTTIEATNIKEEQLESDFYMKATEDSNSSNGQLQDFKLFGSSRVERPMGNSAAKRWKQTKVPIRITQDEFNVTLWSSLNSEDEDEEEELEEPPGCAGTTSVTSSSAAQNEGSPSMPKLIIDEHMINHDVLSDGIGNEYVILPDPFSASAVTDVEEVVNAFMGDVKGEEDSQPLSEQLIYAENQLEEAYGNIELIENMPNNVELIQTPGPGPATVSVALSKVNGCVPNPQPALPKLVLQNSSTNVRLKSAGNQSKVTKRQLTAAAQVATPNPPPPLVASSNPARPQRPTIVTKLPTAVANQTAGNAMGAAGAAGEEDEEPKFRCTHRGCNKEFRNHSAMRKHMHTHGPRGHVCNVCGKSFVESSKLKRHQLVHTGEKPFECTFEGCGKRFSLDFNLRTHVRIHTGDRPYHCPIDGCSKCFAQSTNLKSHMLTHTKPKRKWPRAPQVSNNKTPLVARYGRLEFGEDPRLVYVEQNEEVASVLLDGTSLTS

Target ORF information:

RefSeq Version	NM_001300076.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pleiohomeotic like (phol), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300076.1

ORF Insert Sequence:

ATGACAAGTC ATCATCATCA CGTCGGCGTC GGAGGGAACT TCCAGCCTCA ACTGCATCAG 
ATACGAGCGC CCATCGTGAA GAATCTATCG CTGGCCGCTG CGGCGGTACC GTTGCCCCTG 
CACTCCGCCC CGTCGTCGGC ATACTTCACT CATGCACACC AGCCTACGGC GGCTACCATA 
GTCGAGCAAG ATCCGTACAC CGTTTTGGCG GGCGATCCGA TGGGCGAGGA AAGCGAGAGC 
TTCCTGATCG AGGAGATCAT CGACGACTAT GATGATGAGG CTAATATCGT CGTAGACTCA 
GGCGAATTCT TTGAGCACTA TGAATACTAT CCGGTGGATC GGCTGGACAG ACTTACCTCG 
GTCACGGTGG CTACGGCAGA TGCACTGCCG CCGCCGATCA TCATGCAGCA GACCAGCGAC 
GATGAGGAAT TCATCGAGGA GGATGGACAG CAGATGACAT CCTCCTGCGA TGGCGATGGC 
GATGAGGATG ATGAGGCTAC CGCGCCGGCT ACGACAAATG CCTTCGAGAT TGCCTCCGAG 
ATGCTAACCA CCATCGAGGC CACAAACATC AAGGAGGAGC AGCTCGAGAG CGACTTCTAT 
ATGAAGGCGA CCGAGGATTC AAACAGTTCG AATGGCCAGC TGCAGGATTT TAAGCTATTT 
GGAAGCAGTC GCGTCGAGAG GCCCATGGGA AATTCAGCGG CCAAGCGCTG GAAGCAAACA 
AAGGTACCCA TACGCATAAC GCAGGATGAG TTCAATGTCA CCCTTTGGTC CTCGCTCAAC 
TCGGAGGATG AGGACGAGGA GGAGGAGTTG GAGGAACCAC CTGGTTGTGC TGGCACCACA 
TCCGTGACGT CCTCCTCCGC CGCCCAGAAT GAAGGATCAC CCAGCATGCC AAAGCTTATT 
ATTGACGAGC ACATGATCAA CCACGATGTT TTAAGCGATG GCATCGGCAA CGAGTACGTC 
ATCCTACCCG ATCCATTCAG CGCCTCTGCG GTCACGGATG TGGAGGAGGT GGTTAATGCA 
TTTATGGGCG ATGTCAAAGG CGAGGAGGAC AGCCAGCCGC TAAGTGAACA GTTGATCTAT 
GCGGAAAACC AGTTGGAAGA AGCTTATGGT AACATCGAGC TTATCGAGAA CATGCCGAAT 
AATGTGGAGC TGATACAGAC ACCGGGACCA GGTCCAGCCA CTGTGTCGGT AGCGCTATCG 
AAGGTCAACG GATGTGTACC GAATCCACAG CCGGCTCTCC CAAAACTAGT GCTCCAGAAC 
AGCAGCACCA ATGTGCGACT GAAAAGTGCG GGAAATCAGT CAAAGGTGAC AAAGAGGCAA 
CTCACGGCGG CGGCTCAGGT GGCGACACCA AATCCCCCAC CGCCACTAGT GGCTTCCAGC 
AATCCAGCCC GACCCCAACG ACCCACAATT GTAACCAAAT TACCGACTGC AGTGGCCAAT 
CAGACGGCTG GTAATGCCAT GGGAGCAGCT GGCGCGGCCG GCGAAGAGGA TGAGGAGCCA 
AAGTTTCGCT GCACCCATCG CGGCTGCAAC AAGGAGTTTC GCAACCACTC GGCCATGCGC 
AAGCACATGC ACACGCACGG ACCGCGCGGT CATGTGTGCA ACGTCTGCGG CAAGAGCTTT 
GTAGAGAGCT CCAAGCTAAA GCGCCACCAG CTGGTGCACA CCGGCGAGAA GCCCTTTGAG 
TGCACCTTTG AGGGGTGCGG CAAGCGCTTC TCGCTGGACT TCAATCTGCG CACCCACGTA 
CGGATACACA CTGGCGATAG GCCCTACCAC TGTCCCATTG ATGGCTGCTC CAAGTGCTTC 
GCCCAGTCAA CGAACCTCAA GTCCCACATG CTGACGCACA CGAAGCCCAA AAGGAAGTGG 
CCGCGCGCAC CGCAAGTGAG CAACAACAAG ACACCGCTGG TGGCCAGATA CGGGCGACTG 
GAGTTTGGCG AGGATCCTCG TCTAGTGTAC GTAGAACAGA ACGAGGAGGT GGCGTCGGTG 
CTGCTGGATG GCACGTCCCT AACATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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phol cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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