CG4476 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG4476
Entrez Gene ID	39056
Full Name	CG4476 gene product from transcript CG4476-RB
Synonyms	DmNAT3,Dmel\CG4476,anon-WO0149848.7
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67A3-67A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_140036.2	NP_648293.1	uncharacterized protein

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG1698	NP_610517.1
Drosophila melanogaster (fruit fly)	CG4476	NP_648293.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG4476 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG4476 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18001	NM_140036.2 Latest version!	Drosophila melanogaster uncharacterized protein (CG4476), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG4476 ( NM_140036.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648293.1 Drosophila melanogaster uncharacterized protein (CG4476), mRNA.

Price & Availability↑

CloneID	OFa18001
Clone ID Related Accession (Same CDS sequence)	NM_140036.2
Accession Version	NM_140036.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140036.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAGATT TGAAAACGGA GATCCTAGTG AGCGGCGGCC TGCCGAAACT GGAGACCAGC 
AGGTGTGCCG GCAGTGGCGA GGCGCTGCTA AAGCCATCCT TGCTGCCCAT GGCATTGGAA 
TCGTCTGAGC TCTCTCCGGA TCGGAAGGCC CGCGACAACT GGGGCAGTTC GCTGGAGTTC 
CTGATGTCCT GCATCGCCCT GAGCGTGGGA TTAGGCAACG TGTGGCGGTT TCCATTTACG 
GCCCTGGAAA ACGGCGGAGG GGCATTCCTA ATTCCGTACC TCGTCGTCCT GTTTGTGGTG 
GGAAAGCCGA TTTACTACAT GGAGATGCTG CTGGGACAGT TCTCCAGTCG TGGCATCGTG 
CAGGTCTTTG ACTTTGCGCC CCTCATGAGA GGTGTGGGCT ATGCTCAGCT ATTGGCCCTA 
GGCGTTTTGG CCACGTATTA TGCTTCAGTG ATGGCCCTAA CTCTGCGATA CTTTTTCGAT 
TCCTTCGCAT CGGAGTTGCC CTGGAGCTTC TGTCGCGAGG AGTGGGGTGA CGGGTGCGTA 
AGTGCATCTG GGGGGCAGCC TCTTCAGGGC CAACTGTCGC GTAACTTCAG CTCCTCCACG 
CAACTCTACT TGCAACGCAT CGTGCTGAAT GAAACGGATT CCCTGGAGGA GGGCATCGGA 
TATCCCAGTG GTAGTTTGGC CTTAATGCTG GGCATTTCGT GGCTAACCGT AACACTAATC 
ATCATTCGGG GAGTGAAGAG TTCAGGAAAG GCGGCCTATG TCCTGGCCCT CTTTCCGTAC 
GTTGTAATGT TTATCCTGCT CGTGAGAGCA CTCACATTAC CAGGTGCCTA CGACGGAGTT 
ATGTACTTTC TGACTCCACA GTGGGAGAAA CTTTTGGAGC CGCAGGTGTG GTACAATGCC 
GTCACCCAGG TGTTCTTCTC CCTGGCCGTC TGTTTCGGTG TGATCATCAT GTACTCCTCG 
TACAACCGTT TCGGTCACAA TGTCTATCGG GATGCCAATA TAGTAACCAC CCTGGACACA 
TTCACCTCAC TGCTCTCGGG TGTGATTATC TTTGGAATCC TGGGAAACCT GGCTCACGAA 
TCGGGCACCA AGGACATTGC CAGTGTGGTT AAGGCAGGTC CTGGATTGGC TTTCATCTCC 
TATCCGGATG CCATTGCCAA GTTCAAAATG TTTCCTCAAG TGTTTTCGCT GCTCTTCTTC 
GCCATGCTCT TCATGTTGGG CGTTGGTAGC AATGTGGGCA TGGTCAGCTG CATTATGACC 
GTGCTCAAAG ATCAGTTCGT GAACGTCAAG CTGTGGATTA TAGTGGTCAG CCTTTCGGTG 
ATAGGATTTC TGGTGGGATT GATTTACATC ACTCCCGGAG GTCAACATAT CATCACGCTT 
ATGGACTTTC ACGGCGTCAC CTTTGTCTCC CTGGTATCGG CCATCTTTGA GCTCATTGCC 
GTGGGCTGGA TCTACGGCAC CAAACGTCTC TGCCAAGATG CTGAATACAT GCTGAATATC 
AAGACTTCAA ATTACTATCG GATCTGTTGG TCCATTGTTA CTCCTTTGGT TATGTTGGTC 
ATCCTGGTGT ACAGCTTGCT TACCATGCGT CCACTCAGCT ATAATGGTCA GGAATTTCCA 
TTGGTATATA GAGTTGTTGG TTGGTGTGTA TCGGGCTGTA TTATTGGCCA GCTGTTCTAC 
TGGGCAGGTT ATGCAAACTT CAAGCAACCG AAAGGATCTT TGAAGAGTCG CATTAACAAT 
TCCATTAAGC CGCATTCCGA TTGGGGGCCT TCGGATCCCA AAAAGCTGAT GGATTACCAA 
ATGTTTTTGC GAAACAAGGA TGAGGAAAAT TCCAAAAATG TTAATCGCCG TTGTGTTTGC 
TACACAGCTG GAGATCGAAT TTTCGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648293.1
CDS	347..2236
Translation	MKDLKTEILVSGGLPKLETSRCAGSGEALLKPSLLPMALESSELSPDRKARDNWGSSLEFLMSCIALSVGLGNVWRFPFTALENGGGAFLIPYLVVLFVVGKPIYYMEMLLGQFSSRGIVQVFDFAPLMRGVGYAQLLALGVLATYYASVMALTLRYFFDSFASELPWSFCREEWGDGCVSASGGQPLQGQLSRNFSSSTQLYLQRIVLNETDSLEEGIGYPSGSLALMLGISWLTVTLIIIRGVKSSGKAAYVLALFPYVVMFILLVRALTLPGAYDGVMYFLTPQWEKLLEPQVWYNAVTQVFFSLAVCFGVIIMYSSYNRFGHNVYRDANIVTTLDTFTSLLSGVIIFGILGNLAHESGTKDIASVVKAGPGLAFISYPDAIAKFKMFPQVFSLLFFAMLFMLGVGSNVGMVSCIMTVLKDQFVNVKLWIIVVSLSVIGFLVGLIYITPGGQHIITLMDFHGVTFVSLVSAIFELIAVGWIYGTKRLCQDAEYMLNIKTSNYYRICWSIVTPLVMLVILVYSLLTMRPLSYNGQEFPLVYRVVGWCVSGCIIGQLFYWAGYANFKQPKGSLKSRINNSIKPHSDWGPSDPKKLMDYQMFLRNKDEENSKNVNRRCVCYTAGDRIFG

Target ORF information:

RefSeq Version	NM_140036.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG4476), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140036.2

ORF Insert Sequence:

ATGAAAGATT TGAAAACGGA GATCCTAGTG AGCGGCGGCC TGCCGAAACT GGAGACCAGC 
AGGTGTGCCG GCAGTGGCGA GGCGCTGCTA AAGCCATCCT TGCTGCCCAT GGCATTGGAA 
TCGTCTGAGC TCTCTCCGGA TCGGAAGGCC CGCGACAACT GGGGCAGTTC GCTGGAGTTC 
CTGATGTCCT GCATCGCCCT GAGCGTGGGA TTAGGCAACG TGTGGCGGTT TCCATTTACG 
GCCCTGGAAA ACGGCGGAGG GGCATTCCTA ATTCCGTACC TCGTCGTCCT GTTTGTGGTG 
GGAAAGCCGA TTTACTACAT GGAGATGCTG CTGGGACAGT TCTCCAGTCG TGGCATCGTG 
CAGGTCTTTG ACTTTGCGCC CCTCATGAGA GGTGTGGGCT ATGCTCAGCT ATTGGCCCTA 
GGCGTTTTGG CCACGTATTA TGCTTCAGTG ATGGCCCTAA CTCTGCGATA CTTTTTCGAT 
TCCTTCGCAT CGGAGTTGCC CTGGAGCTTC TGTCGCGAGG AGTGGGGTGA CGGGTGCGTA 
AGTGCATCTG GGGGGCAGCC TCTTCAGGGC CAACTGTCGC GTAACTTCAG CTCCTCCACG 
CAACTCTACT TGCAACGCAT CGTGCTGAAT GAAACGGATT CCCTGGAGGA GGGCATCGGA 
TATCCCAGTG GTAGTTTGGC CTTAATGCTG GGCATTTCGT GGCTAACCGT AACACTAATC 
ATCATTCGGG GAGTGAAGAG TTCAGGAAAG GCGGCCTATG TCCTGGCCCT CTTTCCGTAC 
GTTGTAATGT TTATCCTGCT CGTGAGAGCA CTCACATTAC CAGGTGCCTA CGACGGAGTT 
ATGTACTTTC TGACTCCACA GTGGGAGAAA CTTTTGGAGC CGCAGGTGTG GTACAATGCC 
GTCACCCAGG TGTTCTTCTC CCTGGCCGTC TGTTTCGGTG TGATCATCAT GTACTCCTCG 
TACAACCGTT TCGGTCACAA TGTCTATCGG GATGCCAATA TAGTAACCAC CCTGGACACA 
TTCACCTCAC TGCTCTCGGG TGTGATTATC TTTGGAATCC TGGGAAACCT GGCTCACGAA 
TCGGGCACCA AGGACATTGC CAGTGTGGTT AAGGCAGGTC CTGGATTGGC TTTCATCTCC 
TATCCGGATG CCATTGCCAA GTTCAAAATG TTTCCTCAAG TGTTTTCGCT GCTCTTCTTC 
GCCATGCTCT TCATGTTGGG CGTTGGTAGC AATGTGGGCA TGGTCAGCTG CATTATGACC 
GTGCTCAAAG ATCAGTTCGT GAACGTCAAG CTGTGGATTA TAGTGGTCAG CCTTTCGGTG 
ATAGGATTTC TGGTGGGATT GATTTACATC ACTCCCGGAG GTCAACATAT CATCACGCTT 
ATGGACTTTC ACGGCGTCAC CTTTGTCTCC CTGGTATCGG CCATCTTTGA GCTCATTGCC 
GTGGGCTGGA TCTACGGCAC CAAACGTCTC TGCCAAGATG CTGAATACAT GCTGAATATC 
AAGACTTCAA ATTACTATCG GATCTGTTGG TCCATTGTTA CTCCTTTGGT TATGTTGGTC 
ATCCTGGTGT ACAGCTTGCT TACCATGCGT CCACTCAGCT ATAATGGTCA GGAATTTCCA 
TTGGTATATA GAGTTGTTGG TTGGTGTGTA TCGGGCTGTA TTATTGGCCA GCTGTTCTAC 
TGGGCAGGTT ATGCAAACTT CAAGCAACCG AAAGGATCTT TGAAGAGTCG CATTAACAAT 
TCCATTAAGC CGCATTCCGA TTGGGGGCCT TCGGATCCCA AAAAGCTGAT GGATTACCAA 
ATGTTTTTGC GAAACAAGGA TGAGGAAAAT TCCAAAAATG TTAATCGCCG TTGTGTTTGC 
TACACAGCTG GAGATCGAAT TTTCGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG4476 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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