smg cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following smg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa17974 NM_001144453.2
Latest version!
Drosophila melanogaster smaug (smg), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa17974 NM_168310.3
Latest version!
Drosophila melanogaster smaug (smg), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa17974 NM_079263.3
Latest version!
Drosophila melanogaster smaug (smg), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa17974 NM_168309.3
Latest version!
Drosophila melanogaster smaug (smg), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa17975 NM_001144452.2
Latest version!
Drosophila melanogaster smaug (smg), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa17974
    Clone ID Related Accession (Same CDS sequence) NM_079263.3 , NM_168310.3 , NM_001144453.2 , NM_168309.3
    Accession Version NM_079263.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smaug, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079263.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGTACG CAACTGGAAC TGACAACGCG ATGACCTCCG GCATCAGCGG CCAAACCAAT 
    AACTCTAACA GTGCATCCAA CGAGATGCAG CCAACCACAT CAACGCCTAC TGCAGCCCAC
    AAGGAGGCCA CATCAACGGC CACGACGACG GCGACATACG CCAATGGCAA TCCCAATTCT
    AACGCCAATC CCAGCCAGAG CCAGCCGTCC AATGCGCTGT TTTGCGAGCA GGTGACCACA
    GTGACAAATC TCTTCGAGAA GTGGAACGAC TGCGAGCGAA CCGTCGTGAT GTACGCGCTG
    CTCAAGCGGC TGCGCTACCC GAGCCTCAAG TTTCTACAGT ATTCTATCGA CAGCAACCTG
    ACCCAGAACC TGGGCACCTC CCAGACCAAT CTGAGCAGCG TGGTTATTGA CATCAATGCC
    AACAATCCGG TGTACCTGCA GAATCTACTC AACGCCTACA AGACGTTCCA GCCATGTGAC
    CTGCTCGACG CCATGTCCTC CTCGTCGTCC GACAAGGACT CGATGCCATG CTACGGCAGT
    GACTTCCAGA TCACCACATC GGCGCAGTGT GACGAGCGGA AGCTGTATGC CCGCAAGGAG
    GACATTCTGC ATGAAGTACT GAACATGCTG CCTCTGCTGA AGCCGGGCAA TGAGGAGGCC
    AAGCTTATCT ACCTGACCCT TATACCAGTT GCCGTCAAGG ACACCATGCA GCAAATTGTG
    CCCACGGAGT TGGTGCAGCA GATCTTCTCG TACCTACTCA TCCATCCAGC TATCACCAGC
    GAGGACAGAC GTTCGCTCAA CATTTGGCTG CGTCACTTGG AGGATCATAT CCAAGCGGCT
    GCGGCGGGCC TGACAAATCG CAGTTACTTC CTGCAGCCCT CGCCGCAACT GGTCGCTGGT
    GGTAGCTCAA CAGGCAGTGG TAGCTGTTCC TCTTCGGCGA CCAGCTCTTC GACAGCATCC
    TGTTCGTCGG TGGCCTCATC CTCGTTGTGC CCCGCAAGCG GCAGCCGTTC CTCACGCACC
    AACGACTGGC AAACGATCGC CCCGCCCAGC AAGCAATTGC AAAACAAATT GGCCGGTGAT
    TGGCGTGGCA ATGGAGGCGG CAGCTCCAGC GGGTCGATCA ATCCACTCTG TGATAATCTT
    AATGGTATTA CCCTGAACGA ATTAGCTTCT AGTCAGAACA GTCTCGGCCT GTCGCTGGAG
    GGCAGCTCTT CCCTGGTCAA CGGTGTGGTC GCTGGTGCAG GCTCTATGTT GGGAATCGCT
    GGTGGCGACG ATCATGACAC GTCATTTAGC AAGAATGGCA CGGAGATTCT CGATTTTGAC
    CCGGTGACGG CGGACATGGG TGAGGCCTGT AGTCTGGCCA GCAGTAGCCT TTGTGGACGT
    AACGGTGGAA ATCCTGTTGA GGATCGATCG CAACCTCCGC CGAATCTTCA GCAGCAATTG
    CTTCAGCCTC CTCCCTATGC CTCGATCCTT ATGGGAAACG TGGGCGATCA GTTTGGAGAG
    ATCAACCGGT GGAGCCTGGA CAGCAAGATT GCAGCCCTGA AGACGCGGCG CTCTAACAGC
    CTCACCACGC AAACGATCTC TAGCTGTTCC AGCTCGTCTA ACTCATCGGT GATCACGGTC
    AATGACAACT GCTCCAATTC AACGGAGAAT CTGGCACAGT TTGCCAACAA GCCCCGTAGT
    TTTTCCTTAT CGATTGAGCA TCAGCGCGGC GCCTTGATGA ACAGCGGTTC CGATACTCGT
    CTGGATGAAT TCAAGCCCAA TTATATTAAG TTCCACACGC GCAACGTGGG CATGTCGGGA
    ATCGGACTCT GGCTAAAATC CTTGCGTCTG CACAAGTACA TCGAGCTGTT CAAGAACATG
    ACGTACGAGG AAATGCTCTT GATCACCGAG GACTTTCTGC AGAGTGTTGG CGTCACCAAG
    GGCGCCTCCC ACAAGCTTGC CCTTTGCATT GATAAGCTGA AGGAACGGGC CAATATTCTT
    AACCGGGTAG AACAAGAACT GCTATCGGGT CAAATGGAGC TGAGCACGGC GGTTGAGGAG
    CTAACAAATA TTGTGCTGAC ACCAATGAAG CCACTGGAGT CTCCAGGACC ACCGGAGGAA
    AACATTGGTC TGCGTTTTCT TAAGGTCATT GACATTGTGA CCAATACGTT GCAGCAGGAT
    CCGTATGCTG TCCAAGACGA CGAGACTTTG GGTGTGCTTA TGTGGATTCT AGACCGCTCT
    ATCCACAACG AGGCATTTAT GAATCACGCC AGTCAACTCA AGGACCTGAA GTTTAAGTTG
    TCCAAAATGA AGATCTCCAT GGTTCCCAAA ATGCATCACG TCAAGCCAGC CGGCGTCGGG
    CCAAACAATG GGAACATTAA CAAGCCAAGA TGGAATGGTA AGACTCGCAA ATGCGATACT
    AAAAATGGCA GCAACGATAG GATTAACAAC CGCAAAAACT CCAACGACAT GCTAAACTTT
    TCGCTAAATT GCCTGCCACA CCCGCTGCCC CATCATTCGC AGCAGGCTCC GCCGCCTCTG
    CCACAGTTTG ACTACAACGG CTATGGCGGC GGTCCATCCC ATCAGCCGCA GTACAAGAGC
    TCCTCGTACC CAAGCTTCAT GGGTAATCCT CAGCAGCAGC CACCACCGCC GCCGTCTAGT
    AAGTCGCATC ATCATCCCCA GCAGATGCAA CAGATGTTGC AGCAACACAA CCACTTTCCA
    GCGCTGCCTC AGCAGACGCC ACCACAATCG CATCGCCGGT CGCTGAATAA TCTTATTCTG
    GTGGCCGGAG GTCCGCAGCA ACCGCAGCAA TTGATCTTTA AGCCCGGTCA GGGAGTGCTC
    ACCAACAACG GATCTAACGA CAACCTGGTA TTGGAACGCA ATCAGCAGTC GCAGCAGCAG
    CAGCAGCAGA GAAAACTTAG CGGAGGAGTG TCCAGTGCGG AGCAACAACC AAAGAAGACC
    ATGGCCGCTG TTGTGATGGA AAATTTGGCC AAATTTGATC AACATTTTAC GCTATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_523987.1
    CDS492..3491
    Translation

    Target ORF information:

    RefSeq Version NM_079263.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smaug (smg), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_079263.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGTACG CAACTGGAAC TGACAACGCG ATGACCTCCG GCATCAGCGG CCAAACCAAT 
    AACTCTAACA GTGCATCCAA CGAGATGCAG CCAACCACAT CAACGCCTAC TGCAGCCCAC
    AAGGAGGCCA CATCAACGGC CACGACGACG GCGACATACG CCAATGGCAA TCCCAATTCT
    AACGCCAATC CCAGCCAGAG CCAGCCGTCC AATGCGCTGT TTTGCGAGCA GGTGACCACA
    GTGACAAATC TCTTCGAGAA GTGGAACGAC TGCGAGCGAA CCGTCGTGAT GTACGCGCTG
    CTCAAGCGGC TGCGCTACCC GAGCCTCAAG TTTCTACAGT ATTCTATCGA CAGCAACCTG
    ACCCAGAACC TGGGCACCTC CCAGACCAAT CTGAGCAGCG TGGTTATTGA CATCAATGCC
    AACAATCCGG TGTACCTGCA GAATCTACTC AACGCCTACA AGACGTTCCA GCCATGTGAC
    CTGCTCGACG CCATGTCCTC CTCGTCGTCC GACAAGGACT CGATGCCATG CTACGGCAGT
    GACTTCCAGA TCACCACATC GGCGCAGTGT GACGAGCGGA AGCTGTATGC CCGCAAGGAG
    GACATTCTGC ATGAAGTACT GAACATGCTG CCTCTGCTGA AGCCGGGCAA TGAGGAGGCC
    AAGCTTATCT ACCTGACCCT TATACCAGTT GCCGTCAAGG ACACCATGCA GCAAATTGTG
    CCCACGGAGT TGGTGCAGCA GATCTTCTCG TACCTACTCA TCCATCCAGC TATCACCAGC
    GAGGACAGAC GTTCGCTCAA CATTTGGCTG CGTCACTTGG AGGATCATAT CCAAGCGGCT
    GCGGCGGGCC TGACAAATCG CAGTTACTTC CTGCAGCCCT CGCCGCAACT GGTCGCTGGT
    GGTAGCTCAA CAGGCAGTGG TAGCTGTTCC TCTTCGGCGA CCAGCTCTTC GACAGCATCC
    TGTTCGTCGG TGGCCTCATC CTCGTTGTGC CCCGCAAGCG GCAGCCGTTC CTCACGCACC
    AACGACTGGC AAACGATCGC CCCGCCCAGC AAGCAATTGC AAAACAAATT GGCCGGTGAT
    TGGCGTGGCA ATGGAGGCGG CAGCTCCAGC GGGTCGATCA ATCCACTCTG TGATAATCTT
    AATGGTATTA CCCTGAACGA ATTAGCTTCT AGTCAGAACA GTCTCGGCCT GTCGCTGGAG
    GGCAGCTCTT CCCTGGTCAA CGGTGTGGTC GCTGGTGCAG GCTCTATGTT GGGAATCGCT
    GGTGGCGACG ATCATGACAC GTCATTTAGC AAGAATGGCA CGGAGATTCT CGATTTTGAC
    CCGGTGACGG CGGACATGGG TGAGGCCTGT AGTCTGGCCA GCAGTAGCCT TTGTGGACGT
    AACGGTGGAA ATCCTGTTGA GGATCGATCG CAACCTCCGC CGAATCTTCA GCAGCAATTG
    CTTCAGCCTC CTCCCTATGC CTCGATCCTT ATGGGAAACG TGGGCGATCA GTTTGGAGAG
    ATCAACCGGT GGAGCCTGGA CAGCAAGATT GCAGCCCTGA AGACGCGGCG CTCTAACAGC
    CTCACCACGC AAACGATCTC TAGCTGTTCC AGCTCGTCTA ACTCATCGGT GATCACGGTC
    AATGACAACT GCTCCAATTC AACGGAGAAT CTGGCACAGT TTGCCAACAA GCCCCGTAGT
    TTTTCCTTAT CGATTGAGCA TCAGCGCGGC GCCTTGATGA ACAGCGGTTC CGATACTCGT
    CTGGATGAAT TCAAGCCCAA TTATATTAAG TTCCACACGC GCAACGTGGG CATGTCGGGA
    ATCGGACTCT GGCTAAAATC CTTGCGTCTG CACAAGTACA TCGAGCTGTT CAAGAACATG
    ACGTACGAGG AAATGCTCTT GATCACCGAG GACTTTCTGC AGAGTGTTGG CGTCACCAAG
    GGCGCCTCCC ACAAGCTTGC CCTTTGCATT GATAAGCTGA AGGAACGGGC CAATATTCTT
    AACCGGGTAG AACAAGAACT GCTATCGGGT CAAATGGAGC TGAGCACGGC GGTTGAGGAG
    CTAACAAATA TTGTGCTGAC ACCAATGAAG CCACTGGAGT CTCCAGGACC ACCGGAGGAA
    AACATTGGTC TGCGTTTTCT TAAGGTCATT GACATTGTGA CCAATACGTT GCAGCAGGAT
    CCGTATGCTG TCCAAGACGA CGAGACTTTG GGTGTGCTTA TGTGGATTCT AGACCGCTCT
    ATCCACAACG AGGCATTTAT GAATCACGCC AGTCAACTCA AGGACCTGAA GTTTAAGTTG
    TCCAAAATGA AGATCTCCAT GGTTCCCAAA ATGCATCACG TCAAGCCAGC CGGCGTCGGG
    CCAAACAATG GGAACATTAA CAAGCCAAGA TGGAATGGTA AGACTCGCAA ATGCGATACT
    AAAAATGGCA GCAACGATAG GATTAACAAC CGCAAAAACT CCAACGACAT GCTAAACTTT
    TCGCTAAATT GCCTGCCACA CCCGCTGCCC CATCATTCGC AGCAGGCTCC GCCGCCTCTG
    CCACAGTTTG ACTACAACGG CTATGGCGGC GGTCCATCCC ATCAGCCGCA GTACAAGAGC
    TCCTCGTACC CAAGCTTCAT GGGTAATCCT CAGCAGCAGC CACCACCGCC GCCGTCTAGT
    AAGTCGCATC ATCATCCCCA GCAGATGCAA CAGATGTTGC AGCAACACAA CCACTTTCCA
    GCGCTGCCTC AGCAGACGCC ACCACAATCG CATCGCCGGT CGCTGAATAA TCTTATTCTG
    GTGGCCGGAG GTCCGCAGCA ACCGCAGCAA TTGATCTTTA AGCCCGGTCA GGGAGTGCTC
    ACCAACAACG GATCTAACGA CAACCTGGTA TTGGAACGCA ATCAGCAGTC GCAGCAGCAG
    CAGCAGCAGA GAAAACTTAG CGGAGGAGTG TCCAGTGCGG AGCAACAACC AAAGAAGACC
    ATGGCCGCTG TTGTGATGGA AAATTTGGCC AAATTTGATC AACATTTTAC GCTATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17974
    Clone ID Related Accession (Same CDS sequence) NM_079263.3 , NM_168310.3 , NM_001144453.2 , NM_168309.3
    Accession Version NM_168310.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smaug, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168310.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGTACG CAACTGGAAC TGACAACGCG ATGACCTCCG GCATCAGCGG CCAAACCAAT 
    AACTCTAACA GTGCATCCAA CGAGATGCAG CCAACCACAT CAACGCCTAC TGCAGCCCAC
    AAGGAGGCCA CATCAACGGC CACGACGACG GCGACATACG CCAATGGCAA TCCCAATTCT
    AACGCCAATC CCAGCCAGAG CCAGCCGTCC AATGCGCTGT TTTGCGAGCA GGTGACCACA
    GTGACAAATC TCTTCGAGAA GTGGAACGAC TGCGAGCGAA CCGTCGTGAT GTACGCGCTG
    CTCAAGCGGC TGCGCTACCC GAGCCTCAAG TTTCTACAGT ATTCTATCGA CAGCAACCTG
    ACCCAGAACC TGGGCACCTC CCAGACCAAT CTGAGCAGCG TGGTTATTGA CATCAATGCC
    AACAATCCGG TGTACCTGCA GAATCTACTC AACGCCTACA AGACGTTCCA GCCATGTGAC
    CTGCTCGACG CCATGTCCTC CTCGTCGTCC GACAAGGACT CGATGCCATG CTACGGCAGT
    GACTTCCAGA TCACCACATC GGCGCAGTGT GACGAGCGGA AGCTGTATGC CCGCAAGGAG
    GACATTCTGC ATGAAGTACT GAACATGCTG CCTCTGCTGA AGCCGGGCAA TGAGGAGGCC
    AAGCTTATCT ACCTGACCCT TATACCAGTT GCCGTCAAGG ACACCATGCA GCAAATTGTG
    CCCACGGAGT TGGTGCAGCA GATCTTCTCG TACCTACTCA TCCATCCAGC TATCACCAGC
    GAGGACAGAC GTTCGCTCAA CATTTGGCTG CGTCACTTGG AGGATCATAT CCAAGCGGCT
    GCGGCGGGCC TGACAAATCG CAGTTACTTC CTGCAGCCCT CGCCGCAACT GGTCGCTGGT
    GGTAGCTCAA CAGGCAGTGG TAGCTGTTCC TCTTCGGCGA CCAGCTCTTC GACAGCATCC
    TGTTCGTCGG TGGCCTCATC CTCGTTGTGC CCCGCAAGCG GCAGCCGTTC CTCACGCACC
    AACGACTGGC AAACGATCGC CCCGCCCAGC AAGCAATTGC AAAACAAATT GGCCGGTGAT
    TGGCGTGGCA ATGGAGGCGG CAGCTCCAGC GGGTCGATCA ATCCACTCTG TGATAATCTT
    AATGGTATTA CCCTGAACGA ATTAGCTTCT AGTCAGAACA GTCTCGGCCT GTCGCTGGAG
    GGCAGCTCTT CCCTGGTCAA CGGTGTGGTC GCTGGTGCAG GCTCTATGTT GGGAATCGCT
    GGTGGCGACG ATCATGACAC GTCATTTAGC AAGAATGGCA CGGAGATTCT CGATTTTGAC
    CCGGTGACGG CGGACATGGG TGAGGCCTGT AGTCTGGCCA GCAGTAGCCT TTGTGGACGT
    AACGGTGGAA ATCCTGTTGA GGATCGATCG CAACCTCCGC CGAATCTTCA GCAGCAATTG
    CTTCAGCCTC CTCCCTATGC CTCGATCCTT ATGGGAAACG TGGGCGATCA GTTTGGAGAG
    ATCAACCGGT GGAGCCTGGA CAGCAAGATT GCAGCCCTGA AGACGCGGCG CTCTAACAGC
    CTCACCACGC AAACGATCTC TAGCTGTTCC AGCTCGTCTA ACTCATCGGT GATCACGGTC
    AATGACAACT GCTCCAATTC AACGGAGAAT CTGGCACAGT TTGCCAACAA GCCCCGTAGT
    TTTTCCTTAT CGATTGAGCA TCAGCGCGGC GCCTTGATGA ACAGCGGTTC CGATACTCGT
    CTGGATGAAT TCAAGCCCAA TTATATTAAG TTCCACACGC GCAACGTGGG CATGTCGGGA
    ATCGGACTCT GGCTAAAATC CTTGCGTCTG CACAAGTACA TCGAGCTGTT CAAGAACATG
    ACGTACGAGG AAATGCTCTT GATCACCGAG GACTTTCTGC AGAGTGTTGG CGTCACCAAG
    GGCGCCTCCC ACAAGCTTGC CCTTTGCATT GATAAGCTGA AGGAACGGGC CAATATTCTT
    AACCGGGTAG AACAAGAACT GCTATCGGGT CAAATGGAGC TGAGCACGGC GGTTGAGGAG
    CTAACAAATA TTGTGCTGAC ACCAATGAAG CCACTGGAGT CTCCAGGACC ACCGGAGGAA
    AACATTGGTC TGCGTTTTCT TAAGGTCATT GACATTGTGA CCAATACGTT GCAGCAGGAT
    CCGTATGCTG TCCAAGACGA CGAGACTTTG GGTGTGCTTA TGTGGATTCT AGACCGCTCT
    ATCCACAACG AGGCATTTAT GAATCACGCC AGTCAACTCA AGGACCTGAA GTTTAAGTTG
    TCCAAAATGA AGATCTCCAT GGTTCCCAAA ATGCATCACG TCAAGCCAGC CGGCGTCGGG
    CCAAACAATG GGAACATTAA CAAGCCAAGA TGGAATGGTA AGACTCGCAA ATGCGATACT
    AAAAATGGCA GCAACGATAG GATTAACAAC CGCAAAAACT CCAACGACAT GCTAAACTTT
    TCGCTAAATT GCCTGCCACA CCCGCTGCCC CATCATTCGC AGCAGGCTCC GCCGCCTCTG
    CCACAGTTTG ACTACAACGG CTATGGCGGC GGTCCATCCC ATCAGCCGCA GTACAAGAGC
    TCCTCGTACC CAAGCTTCAT GGGTAATCCT CAGCAGCAGC CACCACCGCC GCCGTCTAGT
    AAGTCGCATC ATCATCCCCA GCAGATGCAA CAGATGTTGC AGCAACACAA CCACTTTCCA
    GCGCTGCCTC AGCAGACGCC ACCACAATCG CATCGCCGGT CGCTGAATAA TCTTATTCTG
    GTGGCCGGAG GTCCGCAGCA ACCGCAGCAA TTGATCTTTA AGCCCGGTCA GGGAGTGCTC
    ACCAACAACG GATCTAACGA CAACCTGGTA TTGGAACGCA ATCAGCAGTC GCAGCAGCAG
    CAGCAGCAGA GAAAACTTAG CGGAGGAGTG TCCAGTGCGG AGCAACAACC AAAGAAGACC
    ATGGCCGCTG TTGTGATGGA AAATTTGGCC AAATTTGATC AACATTTTAC GCTATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_729442.1
    CDS384..3383
    Translation

    Target ORF information:

    RefSeq Version NM_168310.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smaug (smg), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_168310.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGTACG CAACTGGAAC TGACAACGCG ATGACCTCCG GCATCAGCGG CCAAACCAAT 
    AACTCTAACA GTGCATCCAA CGAGATGCAG CCAACCACAT CAACGCCTAC TGCAGCCCAC
    AAGGAGGCCA CATCAACGGC CACGACGACG GCGACATACG CCAATGGCAA TCCCAATTCT
    AACGCCAATC CCAGCCAGAG CCAGCCGTCC AATGCGCTGT TTTGCGAGCA GGTGACCACA
    GTGACAAATC TCTTCGAGAA GTGGAACGAC TGCGAGCGAA CCGTCGTGAT GTACGCGCTG
    CTCAAGCGGC TGCGCTACCC GAGCCTCAAG TTTCTACAGT ATTCTATCGA CAGCAACCTG
    ACCCAGAACC TGGGCACCTC CCAGACCAAT CTGAGCAGCG TGGTTATTGA CATCAATGCC
    AACAATCCGG TGTACCTGCA GAATCTACTC AACGCCTACA AGACGTTCCA GCCATGTGAC
    CTGCTCGACG CCATGTCCTC CTCGTCGTCC GACAAGGACT CGATGCCATG CTACGGCAGT
    GACTTCCAGA TCACCACATC GGCGCAGTGT GACGAGCGGA AGCTGTATGC CCGCAAGGAG
    GACATTCTGC ATGAAGTACT GAACATGCTG CCTCTGCTGA AGCCGGGCAA TGAGGAGGCC
    AAGCTTATCT ACCTGACCCT TATACCAGTT GCCGTCAAGG ACACCATGCA GCAAATTGTG
    CCCACGGAGT TGGTGCAGCA GATCTTCTCG TACCTACTCA TCCATCCAGC TATCACCAGC
    GAGGACAGAC GTTCGCTCAA CATTTGGCTG CGTCACTTGG AGGATCATAT CCAAGCGGCT
    GCGGCGGGCC TGACAAATCG CAGTTACTTC CTGCAGCCCT CGCCGCAACT GGTCGCTGGT
    GGTAGCTCAA CAGGCAGTGG TAGCTGTTCC TCTTCGGCGA CCAGCTCTTC GACAGCATCC
    TGTTCGTCGG TGGCCTCATC CTCGTTGTGC CCCGCAAGCG GCAGCCGTTC CTCACGCACC
    AACGACTGGC AAACGATCGC CCCGCCCAGC AAGCAATTGC AAAACAAATT GGCCGGTGAT
    TGGCGTGGCA ATGGAGGCGG CAGCTCCAGC GGGTCGATCA ATCCACTCTG TGATAATCTT
    AATGGTATTA CCCTGAACGA ATTAGCTTCT AGTCAGAACA GTCTCGGCCT GTCGCTGGAG
    GGCAGCTCTT CCCTGGTCAA CGGTGTGGTC GCTGGTGCAG GCTCTATGTT GGGAATCGCT
    GGTGGCGACG ATCATGACAC GTCATTTAGC AAGAATGGCA CGGAGATTCT CGATTTTGAC
    CCGGTGACGG CGGACATGGG TGAGGCCTGT AGTCTGGCCA GCAGTAGCCT TTGTGGACGT
    AACGGTGGAA ATCCTGTTGA GGATCGATCG CAACCTCCGC CGAATCTTCA GCAGCAATTG
    CTTCAGCCTC CTCCCTATGC CTCGATCCTT ATGGGAAACG TGGGCGATCA GTTTGGAGAG
    ATCAACCGGT GGAGCCTGGA CAGCAAGATT GCAGCCCTGA AGACGCGGCG CTCTAACAGC
    CTCACCACGC AAACGATCTC TAGCTGTTCC AGCTCGTCTA ACTCATCGGT GATCACGGTC
    AATGACAACT GCTCCAATTC AACGGAGAAT CTGGCACAGT TTGCCAACAA GCCCCGTAGT
    TTTTCCTTAT CGATTGAGCA TCAGCGCGGC GCCTTGATGA ACAGCGGTTC CGATACTCGT
    CTGGATGAAT TCAAGCCCAA TTATATTAAG TTCCACACGC GCAACGTGGG CATGTCGGGA
    ATCGGACTCT GGCTAAAATC CTTGCGTCTG CACAAGTACA TCGAGCTGTT CAAGAACATG
    ACGTACGAGG AAATGCTCTT GATCACCGAG GACTTTCTGC AGAGTGTTGG CGTCACCAAG
    GGCGCCTCCC ACAAGCTTGC CCTTTGCATT GATAAGCTGA AGGAACGGGC CAATATTCTT
    AACCGGGTAG AACAAGAACT GCTATCGGGT CAAATGGAGC TGAGCACGGC GGTTGAGGAG
    CTAACAAATA TTGTGCTGAC ACCAATGAAG CCACTGGAGT CTCCAGGACC ACCGGAGGAA
    AACATTGGTC TGCGTTTTCT TAAGGTCATT GACATTGTGA CCAATACGTT GCAGCAGGAT
    CCGTATGCTG TCCAAGACGA CGAGACTTTG GGTGTGCTTA TGTGGATTCT AGACCGCTCT
    ATCCACAACG AGGCATTTAT GAATCACGCC AGTCAACTCA AGGACCTGAA GTTTAAGTTG
    TCCAAAATGA AGATCTCCAT GGTTCCCAAA ATGCATCACG TCAAGCCAGC CGGCGTCGGG
    CCAAACAATG GGAACATTAA CAAGCCAAGA TGGAATGGTA AGACTCGCAA ATGCGATACT
    AAAAATGGCA GCAACGATAG GATTAACAAC CGCAAAAACT CCAACGACAT GCTAAACTTT
    TCGCTAAATT GCCTGCCACA CCCGCTGCCC CATCATTCGC AGCAGGCTCC GCCGCCTCTG
    CCACAGTTTG ACTACAACGG CTATGGCGGC GGTCCATCCC ATCAGCCGCA GTACAAGAGC
    TCCTCGTACC CAAGCTTCAT GGGTAATCCT CAGCAGCAGC CACCACCGCC GCCGTCTAGT
    AAGTCGCATC ATCATCCCCA GCAGATGCAA CAGATGTTGC AGCAACACAA CCACTTTCCA
    GCGCTGCCTC AGCAGACGCC ACCACAATCG CATCGCCGGT CGCTGAATAA TCTTATTCTG
    GTGGCCGGAG GTCCGCAGCA ACCGCAGCAA TTGATCTTTA AGCCCGGTCA GGGAGTGCTC
    ACCAACAACG GATCTAACGA CAACCTGGTA TTGGAACGCA ATCAGCAGTC GCAGCAGCAG
    CAGCAGCAGA GAAAACTTAG CGGAGGAGTG TCCAGTGCGG AGCAACAACC AAAGAAGACC
    ATGGCCGCTG TTGTGATGGA AAATTTGGCC AAATTTGATC AACATTTTAC GCTATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17974
    Clone ID Related Accession (Same CDS sequence) NM_079263.3 , NM_168310.3 , NM_001144453.2 , NM_168309.3
    Accession Version NM_001144453.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smaug, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001144453.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGTACG CAACTGGAAC TGACAACGCG ATGACCTCCG GCATCAGCGG CCAAACCAAT 
    AACTCTAACA GTGCATCCAA CGAGATGCAG CCAACCACAT CAACGCCTAC TGCAGCCCAC
    AAGGAGGCCA CATCAACGGC CACGACGACG GCGACATACG CCAATGGCAA TCCCAATTCT
    AACGCCAATC CCAGCCAGAG CCAGCCGTCC AATGCGCTGT TTTGCGAGCA GGTGACCACA
    GTGACAAATC TCTTCGAGAA GTGGAACGAC TGCGAGCGAA CCGTCGTGAT GTACGCGCTG
    CTCAAGCGGC TGCGCTACCC GAGCCTCAAG TTTCTACAGT ATTCTATCGA CAGCAACCTG
    ACCCAGAACC TGGGCACCTC CCAGACCAAT CTGAGCAGCG TGGTTATTGA CATCAATGCC
    AACAATCCGG TGTACCTGCA GAATCTACTC AACGCCTACA AGACGTTCCA GCCATGTGAC
    CTGCTCGACG CCATGTCCTC CTCGTCGTCC GACAAGGACT CGATGCCATG CTACGGCAGT
    GACTTCCAGA TCACCACATC GGCGCAGTGT GACGAGCGGA AGCTGTATGC CCGCAAGGAG
    GACATTCTGC ATGAAGTACT GAACATGCTG CCTCTGCTGA AGCCGGGCAA TGAGGAGGCC
    AAGCTTATCT ACCTGACCCT TATACCAGTT GCCGTCAAGG ACACCATGCA GCAAATTGTG
    CCCACGGAGT TGGTGCAGCA GATCTTCTCG TACCTACTCA TCCATCCAGC TATCACCAGC
    GAGGACAGAC GTTCGCTCAA CATTTGGCTG CGTCACTTGG AGGATCATAT CCAAGCGGCT
    GCGGCGGGCC TGACAAATCG CAGTTACTTC CTGCAGCCCT CGCCGCAACT GGTCGCTGGT
    GGTAGCTCAA CAGGCAGTGG TAGCTGTTCC TCTTCGGCGA CCAGCTCTTC GACAGCATCC
    TGTTCGTCGG TGGCCTCATC CTCGTTGTGC CCCGCAAGCG GCAGCCGTTC CTCACGCACC
    AACGACTGGC AAACGATCGC CCCGCCCAGC AAGCAATTGC AAAACAAATT GGCCGGTGAT
    TGGCGTGGCA ATGGAGGCGG CAGCTCCAGC GGGTCGATCA ATCCACTCTG TGATAATCTT
    AATGGTATTA CCCTGAACGA ATTAGCTTCT AGTCAGAACA GTCTCGGCCT GTCGCTGGAG
    GGCAGCTCTT CCCTGGTCAA CGGTGTGGTC GCTGGTGCAG GCTCTATGTT GGGAATCGCT
    GGTGGCGACG ATCATGACAC GTCATTTAGC AAGAATGGCA CGGAGATTCT CGATTTTGAC
    CCGGTGACGG CGGACATGGG TGAGGCCTGT AGTCTGGCCA GCAGTAGCCT TTGTGGACGT
    AACGGTGGAA ATCCTGTTGA GGATCGATCG CAACCTCCGC CGAATCTTCA GCAGCAATTG
    CTTCAGCCTC CTCCCTATGC CTCGATCCTT ATGGGAAACG TGGGCGATCA GTTTGGAGAG
    ATCAACCGGT GGAGCCTGGA CAGCAAGATT GCAGCCCTGA AGACGCGGCG CTCTAACAGC
    CTCACCACGC AAACGATCTC TAGCTGTTCC AGCTCGTCTA ACTCATCGGT GATCACGGTC
    AATGACAACT GCTCCAATTC AACGGAGAAT CTGGCACAGT TTGCCAACAA GCCCCGTAGT
    TTTTCCTTAT CGATTGAGCA TCAGCGCGGC GCCTTGATGA ACAGCGGTTC CGATACTCGT
    CTGGATGAAT TCAAGCCCAA TTATATTAAG TTCCACACGC GCAACGTGGG CATGTCGGGA
    ATCGGACTCT GGCTAAAATC CTTGCGTCTG CACAAGTACA TCGAGCTGTT CAAGAACATG
    ACGTACGAGG AAATGCTCTT GATCACCGAG GACTTTCTGC AGAGTGTTGG CGTCACCAAG
    GGCGCCTCCC ACAAGCTTGC CCTTTGCATT GATAAGCTGA AGGAACGGGC CAATATTCTT
    AACCGGGTAG AACAAGAACT GCTATCGGGT CAAATGGAGC TGAGCACGGC GGTTGAGGAG
    CTAACAAATA TTGTGCTGAC ACCAATGAAG CCACTGGAGT CTCCAGGACC ACCGGAGGAA
    AACATTGGTC TGCGTTTTCT TAAGGTCATT GACATTGTGA CCAATACGTT GCAGCAGGAT
    CCGTATGCTG TCCAAGACGA CGAGACTTTG GGTGTGCTTA TGTGGATTCT AGACCGCTCT
    ATCCACAACG AGGCATTTAT GAATCACGCC AGTCAACTCA AGGACCTGAA GTTTAAGTTG
    TCCAAAATGA AGATCTCCAT GGTTCCCAAA ATGCATCACG TCAAGCCAGC CGGCGTCGGG
    CCAAACAATG GGAACATTAA CAAGCCAAGA TGGAATGGTA AGACTCGCAA ATGCGATACT
    AAAAATGGCA GCAACGATAG GATTAACAAC CGCAAAAACT CCAACGACAT GCTAAACTTT
    TCGCTAAATT GCCTGCCACA CCCGCTGCCC CATCATTCGC AGCAGGCTCC GCCGCCTCTG
    CCACAGTTTG ACTACAACGG CTATGGCGGC GGTCCATCCC ATCAGCCGCA GTACAAGAGC
    TCCTCGTACC CAAGCTTCAT GGGTAATCCT CAGCAGCAGC CACCACCGCC GCCGTCTAGT
    AAGTCGCATC ATCATCCCCA GCAGATGCAA CAGATGTTGC AGCAACACAA CCACTTTCCA
    GCGCTGCCTC AGCAGACGCC ACCACAATCG CATCGCCGGT CGCTGAATAA TCTTATTCTG
    GTGGCCGGAG GTCCGCAGCA ACCGCAGCAA TTGATCTTTA AGCCCGGTCA GGGAGTGCTC
    ACCAACAACG GATCTAACGA CAACCTGGTA TTGGAACGCA ATCAGCAGTC GCAGCAGCAG
    CAGCAGCAGA GAAAACTTAG CGGAGGAGTG TCCAGTGCGG AGCAACAACC AAAGAAGACC
    ATGGCCGCTG TTGTGATGGA AAATTTGGCC AAATTTGATC AACATTTTAC GCTATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137925.1
    CDS287..3286
    Translation

    Target ORF information:

    RefSeq Version NM_001144453.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smaug (smg), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144453.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGTACG CAACTGGAAC TGACAACGCG ATGACCTCCG GCATCAGCGG CCAAACCAAT 
    AACTCTAACA GTGCATCCAA CGAGATGCAG CCAACCACAT CAACGCCTAC TGCAGCCCAC
    AAGGAGGCCA CATCAACGGC CACGACGACG GCGACATACG CCAATGGCAA TCCCAATTCT
    AACGCCAATC CCAGCCAGAG CCAGCCGTCC AATGCGCTGT TTTGCGAGCA GGTGACCACA
    GTGACAAATC TCTTCGAGAA GTGGAACGAC TGCGAGCGAA CCGTCGTGAT GTACGCGCTG
    CTCAAGCGGC TGCGCTACCC GAGCCTCAAG TTTCTACAGT ATTCTATCGA CAGCAACCTG
    ACCCAGAACC TGGGCACCTC CCAGACCAAT CTGAGCAGCG TGGTTATTGA CATCAATGCC
    AACAATCCGG TGTACCTGCA GAATCTACTC AACGCCTACA AGACGTTCCA GCCATGTGAC
    CTGCTCGACG CCATGTCCTC CTCGTCGTCC GACAAGGACT CGATGCCATG CTACGGCAGT
    GACTTCCAGA TCACCACATC GGCGCAGTGT GACGAGCGGA AGCTGTATGC CCGCAAGGAG
    GACATTCTGC ATGAAGTACT GAACATGCTG CCTCTGCTGA AGCCGGGCAA TGAGGAGGCC
    AAGCTTATCT ACCTGACCCT TATACCAGTT GCCGTCAAGG ACACCATGCA GCAAATTGTG
    CCCACGGAGT TGGTGCAGCA GATCTTCTCG TACCTACTCA TCCATCCAGC TATCACCAGC
    GAGGACAGAC GTTCGCTCAA CATTTGGCTG CGTCACTTGG AGGATCATAT CCAAGCGGCT
    GCGGCGGGCC TGACAAATCG CAGTTACTTC CTGCAGCCCT CGCCGCAACT GGTCGCTGGT
    GGTAGCTCAA CAGGCAGTGG TAGCTGTTCC TCTTCGGCGA CCAGCTCTTC GACAGCATCC
    TGTTCGTCGG TGGCCTCATC CTCGTTGTGC CCCGCAAGCG GCAGCCGTTC CTCACGCACC
    AACGACTGGC AAACGATCGC CCCGCCCAGC AAGCAATTGC AAAACAAATT GGCCGGTGAT
    TGGCGTGGCA ATGGAGGCGG CAGCTCCAGC GGGTCGATCA ATCCACTCTG TGATAATCTT
    AATGGTATTA CCCTGAACGA ATTAGCTTCT AGTCAGAACA GTCTCGGCCT GTCGCTGGAG
    GGCAGCTCTT CCCTGGTCAA CGGTGTGGTC GCTGGTGCAG GCTCTATGTT GGGAATCGCT
    GGTGGCGACG ATCATGACAC GTCATTTAGC AAGAATGGCA CGGAGATTCT CGATTTTGAC
    CCGGTGACGG CGGACATGGG TGAGGCCTGT AGTCTGGCCA GCAGTAGCCT TTGTGGACGT
    AACGGTGGAA ATCCTGTTGA GGATCGATCG CAACCTCCGC CGAATCTTCA GCAGCAATTG
    CTTCAGCCTC CTCCCTATGC CTCGATCCTT ATGGGAAACG TGGGCGATCA GTTTGGAGAG
    ATCAACCGGT GGAGCCTGGA CAGCAAGATT GCAGCCCTGA AGACGCGGCG CTCTAACAGC
    CTCACCACGC AAACGATCTC TAGCTGTTCC AGCTCGTCTA ACTCATCGGT GATCACGGTC
    AATGACAACT GCTCCAATTC AACGGAGAAT CTGGCACAGT TTGCCAACAA GCCCCGTAGT
    TTTTCCTTAT CGATTGAGCA TCAGCGCGGC GCCTTGATGA ACAGCGGTTC CGATACTCGT
    CTGGATGAAT TCAAGCCCAA TTATATTAAG TTCCACACGC GCAACGTGGG CATGTCGGGA
    ATCGGACTCT GGCTAAAATC CTTGCGTCTG CACAAGTACA TCGAGCTGTT CAAGAACATG
    ACGTACGAGG AAATGCTCTT GATCACCGAG GACTTTCTGC AGAGTGTTGG CGTCACCAAG
    GGCGCCTCCC ACAAGCTTGC CCTTTGCATT GATAAGCTGA AGGAACGGGC CAATATTCTT
    AACCGGGTAG AACAAGAACT GCTATCGGGT CAAATGGAGC TGAGCACGGC GGTTGAGGAG
    CTAACAAATA TTGTGCTGAC ACCAATGAAG CCACTGGAGT CTCCAGGACC ACCGGAGGAA
    AACATTGGTC TGCGTTTTCT TAAGGTCATT GACATTGTGA CCAATACGTT GCAGCAGGAT
    CCGTATGCTG TCCAAGACGA CGAGACTTTG GGTGTGCTTA TGTGGATTCT AGACCGCTCT
    ATCCACAACG AGGCATTTAT GAATCACGCC AGTCAACTCA AGGACCTGAA GTTTAAGTTG
    TCCAAAATGA AGATCTCCAT GGTTCCCAAA ATGCATCACG TCAAGCCAGC CGGCGTCGGG
    CCAAACAATG GGAACATTAA CAAGCCAAGA TGGAATGGTA AGACTCGCAA ATGCGATACT
    AAAAATGGCA GCAACGATAG GATTAACAAC CGCAAAAACT CCAACGACAT GCTAAACTTT
    TCGCTAAATT GCCTGCCACA CCCGCTGCCC CATCATTCGC AGCAGGCTCC GCCGCCTCTG
    CCACAGTTTG ACTACAACGG CTATGGCGGC GGTCCATCCC ATCAGCCGCA GTACAAGAGC
    TCCTCGTACC CAAGCTTCAT GGGTAATCCT CAGCAGCAGC CACCACCGCC GCCGTCTAGT
    AAGTCGCATC ATCATCCCCA GCAGATGCAA CAGATGTTGC AGCAACACAA CCACTTTCCA
    GCGCTGCCTC AGCAGACGCC ACCACAATCG CATCGCCGGT CGCTGAATAA TCTTATTCTG
    GTGGCCGGAG GTCCGCAGCA ACCGCAGCAA TTGATCTTTA AGCCCGGTCA GGGAGTGCTC
    ACCAACAACG GATCTAACGA CAACCTGGTA TTGGAACGCA ATCAGCAGTC GCAGCAGCAG
    CAGCAGCAGA GAAAACTTAG CGGAGGAGTG TCCAGTGCGG AGCAACAACC AAAGAAGACC
    ATGGCCGCTG TTGTGATGGA AAATTTGGCC AAATTTGATC AACATTTTAC GCTATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17974
    Clone ID Related Accession (Same CDS sequence) NM_079263.3 , NM_168310.3 , NM_001144453.2 , NM_168309.3
    Accession Version NM_168309.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smaug, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168309.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGTACG CAACTGGAAC TGACAACGCG ATGACCTCCG GCATCAGCGG CCAAACCAAT 
    AACTCTAACA GTGCATCCAA CGAGATGCAG CCAACCACAT CAACGCCTAC TGCAGCCCAC
    AAGGAGGCCA CATCAACGGC CACGACGACG GCGACATACG CCAATGGCAA TCCCAATTCT
    AACGCCAATC CCAGCCAGAG CCAGCCGTCC AATGCGCTGT TTTGCGAGCA GGTGACCACA
    GTGACAAATC TCTTCGAGAA GTGGAACGAC TGCGAGCGAA CCGTCGTGAT GTACGCGCTG
    CTCAAGCGGC TGCGCTACCC GAGCCTCAAG TTTCTACAGT ATTCTATCGA CAGCAACCTG
    ACCCAGAACC TGGGCACCTC CCAGACCAAT CTGAGCAGCG TGGTTATTGA CATCAATGCC
    AACAATCCGG TGTACCTGCA GAATCTACTC AACGCCTACA AGACGTTCCA GCCATGTGAC
    CTGCTCGACG CCATGTCCTC CTCGTCGTCC GACAAGGACT CGATGCCATG CTACGGCAGT
    GACTTCCAGA TCACCACATC GGCGCAGTGT GACGAGCGGA AGCTGTATGC CCGCAAGGAG
    GACATTCTGC ATGAAGTACT GAACATGCTG CCTCTGCTGA AGCCGGGCAA TGAGGAGGCC
    AAGCTTATCT ACCTGACCCT TATACCAGTT GCCGTCAAGG ACACCATGCA GCAAATTGTG
    CCCACGGAGT TGGTGCAGCA GATCTTCTCG TACCTACTCA TCCATCCAGC TATCACCAGC
    GAGGACAGAC GTTCGCTCAA CATTTGGCTG CGTCACTTGG AGGATCATAT CCAAGCGGCT
    GCGGCGGGCC TGACAAATCG CAGTTACTTC CTGCAGCCCT CGCCGCAACT GGTCGCTGGT
    GGTAGCTCAA CAGGCAGTGG TAGCTGTTCC TCTTCGGCGA CCAGCTCTTC GACAGCATCC
    TGTTCGTCGG TGGCCTCATC CTCGTTGTGC CCCGCAAGCG GCAGCCGTTC CTCACGCACC
    AACGACTGGC AAACGATCGC CCCGCCCAGC AAGCAATTGC AAAACAAATT GGCCGGTGAT
    TGGCGTGGCA ATGGAGGCGG CAGCTCCAGC GGGTCGATCA ATCCACTCTG TGATAATCTT
    AATGGTATTA CCCTGAACGA ATTAGCTTCT AGTCAGAACA GTCTCGGCCT GTCGCTGGAG
    GGCAGCTCTT CCCTGGTCAA CGGTGTGGTC GCTGGTGCAG GCTCTATGTT GGGAATCGCT
    GGTGGCGACG ATCATGACAC GTCATTTAGC AAGAATGGCA CGGAGATTCT CGATTTTGAC
    CCGGTGACGG CGGACATGGG TGAGGCCTGT AGTCTGGCCA GCAGTAGCCT TTGTGGACGT
    AACGGTGGAA ATCCTGTTGA GGATCGATCG CAACCTCCGC CGAATCTTCA GCAGCAATTG
    CTTCAGCCTC CTCCCTATGC CTCGATCCTT ATGGGAAACG TGGGCGATCA GTTTGGAGAG
    ATCAACCGGT GGAGCCTGGA CAGCAAGATT GCAGCCCTGA AGACGCGGCG CTCTAACAGC
    CTCACCACGC AAACGATCTC TAGCTGTTCC AGCTCGTCTA ACTCATCGGT GATCACGGTC
    AATGACAACT GCTCCAATTC AACGGAGAAT CTGGCACAGT TTGCCAACAA GCCCCGTAGT
    TTTTCCTTAT CGATTGAGCA TCAGCGCGGC GCCTTGATGA ACAGCGGTTC CGATACTCGT
    CTGGATGAAT TCAAGCCCAA TTATATTAAG TTCCACACGC GCAACGTGGG CATGTCGGGA
    ATCGGACTCT GGCTAAAATC CTTGCGTCTG CACAAGTACA TCGAGCTGTT CAAGAACATG
    ACGTACGAGG AAATGCTCTT GATCACCGAG GACTTTCTGC AGAGTGTTGG CGTCACCAAG
    GGCGCCTCCC ACAAGCTTGC CCTTTGCATT GATAAGCTGA AGGAACGGGC CAATATTCTT
    AACCGGGTAG AACAAGAACT GCTATCGGGT CAAATGGAGC TGAGCACGGC GGTTGAGGAG
    CTAACAAATA TTGTGCTGAC ACCAATGAAG CCACTGGAGT CTCCAGGACC ACCGGAGGAA
    AACATTGGTC TGCGTTTTCT TAAGGTCATT GACATTGTGA CCAATACGTT GCAGCAGGAT
    CCGTATGCTG TCCAAGACGA CGAGACTTTG GGTGTGCTTA TGTGGATTCT AGACCGCTCT
    ATCCACAACG AGGCATTTAT GAATCACGCC AGTCAACTCA AGGACCTGAA GTTTAAGTTG
    TCCAAAATGA AGATCTCCAT GGTTCCCAAA ATGCATCACG TCAAGCCAGC CGGCGTCGGG
    CCAAACAATG GGAACATTAA CAAGCCAAGA TGGAATGGTA AGACTCGCAA ATGCGATACT
    AAAAATGGCA GCAACGATAG GATTAACAAC CGCAAAAACT CCAACGACAT GCTAAACTTT
    TCGCTAAATT GCCTGCCACA CCCGCTGCCC CATCATTCGC AGCAGGCTCC GCCGCCTCTG
    CCACAGTTTG ACTACAACGG CTATGGCGGC GGTCCATCCC ATCAGCCGCA GTACAAGAGC
    TCCTCGTACC CAAGCTTCAT GGGTAATCCT CAGCAGCAGC CACCACCGCC GCCGTCTAGT
    AAGTCGCATC ATCATCCCCA GCAGATGCAA CAGATGTTGC AGCAACACAA CCACTTTCCA
    GCGCTGCCTC AGCAGACGCC ACCACAATCG CATCGCCGGT CGCTGAATAA TCTTATTCTG
    GTGGCCGGAG GTCCGCAGCA ACCGCAGCAA TTGATCTTTA AGCCCGGTCA GGGAGTGCTC
    ACCAACAACG GATCTAACGA CAACCTGGTA TTGGAACGCA ATCAGCAGTC GCAGCAGCAG
    CAGCAGCAGA GAAAACTTAG CGGAGGAGTG TCCAGTGCGG AGCAACAACC AAAGAAGACC
    ATGGCCGCTG TTGTGATGGA AAATTTGGCC AAATTTGATC AACATTTTAC GCTATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_729441.1
    CDS510..3509
    Translation

    Target ORF information:

    RefSeq Version NM_168309.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smaug (smg), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_168309.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGTACG CAACTGGAAC TGACAACGCG ATGACCTCCG GCATCAGCGG CCAAACCAAT 
    AACTCTAACA GTGCATCCAA CGAGATGCAG CCAACCACAT CAACGCCTAC TGCAGCCCAC
    AAGGAGGCCA CATCAACGGC CACGACGACG GCGACATACG CCAATGGCAA TCCCAATTCT
    AACGCCAATC CCAGCCAGAG CCAGCCGTCC AATGCGCTGT TTTGCGAGCA GGTGACCACA
    GTGACAAATC TCTTCGAGAA GTGGAACGAC TGCGAGCGAA CCGTCGTGAT GTACGCGCTG
    CTCAAGCGGC TGCGCTACCC GAGCCTCAAG TTTCTACAGT ATTCTATCGA CAGCAACCTG
    ACCCAGAACC TGGGCACCTC CCAGACCAAT CTGAGCAGCG TGGTTATTGA CATCAATGCC
    AACAATCCGG TGTACCTGCA GAATCTACTC AACGCCTACA AGACGTTCCA GCCATGTGAC
    CTGCTCGACG CCATGTCCTC CTCGTCGTCC GACAAGGACT CGATGCCATG CTACGGCAGT
    GACTTCCAGA TCACCACATC GGCGCAGTGT GACGAGCGGA AGCTGTATGC CCGCAAGGAG
    GACATTCTGC ATGAAGTACT GAACATGCTG CCTCTGCTGA AGCCGGGCAA TGAGGAGGCC
    AAGCTTATCT ACCTGACCCT TATACCAGTT GCCGTCAAGG ACACCATGCA GCAAATTGTG
    CCCACGGAGT TGGTGCAGCA GATCTTCTCG TACCTACTCA TCCATCCAGC TATCACCAGC
    GAGGACAGAC GTTCGCTCAA CATTTGGCTG CGTCACTTGG AGGATCATAT CCAAGCGGCT
    GCGGCGGGCC TGACAAATCG CAGTTACTTC CTGCAGCCCT CGCCGCAACT GGTCGCTGGT
    GGTAGCTCAA CAGGCAGTGG TAGCTGTTCC TCTTCGGCGA CCAGCTCTTC GACAGCATCC
    TGTTCGTCGG TGGCCTCATC CTCGTTGTGC CCCGCAAGCG GCAGCCGTTC CTCACGCACC
    AACGACTGGC AAACGATCGC CCCGCCCAGC AAGCAATTGC AAAACAAATT GGCCGGTGAT
    TGGCGTGGCA ATGGAGGCGG CAGCTCCAGC GGGTCGATCA ATCCACTCTG TGATAATCTT
    AATGGTATTA CCCTGAACGA ATTAGCTTCT AGTCAGAACA GTCTCGGCCT GTCGCTGGAG
    GGCAGCTCTT CCCTGGTCAA CGGTGTGGTC GCTGGTGCAG GCTCTATGTT GGGAATCGCT
    GGTGGCGACG ATCATGACAC GTCATTTAGC AAGAATGGCA CGGAGATTCT CGATTTTGAC
    CCGGTGACGG CGGACATGGG TGAGGCCTGT AGTCTGGCCA GCAGTAGCCT TTGTGGACGT
    AACGGTGGAA ATCCTGTTGA GGATCGATCG CAACCTCCGC CGAATCTTCA GCAGCAATTG
    CTTCAGCCTC CTCCCTATGC CTCGATCCTT ATGGGAAACG TGGGCGATCA GTTTGGAGAG
    ATCAACCGGT GGAGCCTGGA CAGCAAGATT GCAGCCCTGA AGACGCGGCG CTCTAACAGC
    CTCACCACGC AAACGATCTC TAGCTGTTCC AGCTCGTCTA ACTCATCGGT GATCACGGTC
    AATGACAACT GCTCCAATTC AACGGAGAAT CTGGCACAGT TTGCCAACAA GCCCCGTAGT
    TTTTCCTTAT CGATTGAGCA TCAGCGCGGC GCCTTGATGA ACAGCGGTTC CGATACTCGT
    CTGGATGAAT TCAAGCCCAA TTATATTAAG TTCCACACGC GCAACGTGGG CATGTCGGGA
    ATCGGACTCT GGCTAAAATC CTTGCGTCTG CACAAGTACA TCGAGCTGTT CAAGAACATG
    ACGTACGAGG AAATGCTCTT GATCACCGAG GACTTTCTGC AGAGTGTTGG CGTCACCAAG
    GGCGCCTCCC ACAAGCTTGC CCTTTGCATT GATAAGCTGA AGGAACGGGC CAATATTCTT
    AACCGGGTAG AACAAGAACT GCTATCGGGT CAAATGGAGC TGAGCACGGC GGTTGAGGAG
    CTAACAAATA TTGTGCTGAC ACCAATGAAG CCACTGGAGT CTCCAGGACC ACCGGAGGAA
    AACATTGGTC TGCGTTTTCT TAAGGTCATT GACATTGTGA CCAATACGTT GCAGCAGGAT
    CCGTATGCTG TCCAAGACGA CGAGACTTTG GGTGTGCTTA TGTGGATTCT AGACCGCTCT
    ATCCACAACG AGGCATTTAT GAATCACGCC AGTCAACTCA AGGACCTGAA GTTTAAGTTG
    TCCAAAATGA AGATCTCCAT GGTTCCCAAA ATGCATCACG TCAAGCCAGC CGGCGTCGGG
    CCAAACAATG GGAACATTAA CAAGCCAAGA TGGAATGGTA AGACTCGCAA ATGCGATACT
    AAAAATGGCA GCAACGATAG GATTAACAAC CGCAAAAACT CCAACGACAT GCTAAACTTT
    TCGCTAAATT GCCTGCCACA CCCGCTGCCC CATCATTCGC AGCAGGCTCC GCCGCCTCTG
    CCACAGTTTG ACTACAACGG CTATGGCGGC GGTCCATCCC ATCAGCCGCA GTACAAGAGC
    TCCTCGTACC CAAGCTTCAT GGGTAATCCT CAGCAGCAGC CACCACCGCC GCCGTCTAGT
    AAGTCGCATC ATCATCCCCA GCAGATGCAA CAGATGTTGC AGCAACACAA CCACTTTCCA
    GCGCTGCCTC AGCAGACGCC ACCACAATCG CATCGCCGGT CGCTGAATAA TCTTATTCTG
    GTGGCCGGAG GTCCGCAGCA ACCGCAGCAA TTGATCTTTA AGCCCGGTCA GGGAGTGCTC
    ACCAACAACG GATCTAACGA CAACCTGGTA TTGGAACGCA ATCAGCAGTC GCAGCAGCAG
    CAGCAGCAGA GAAAACTTAG CGGAGGAGTG TCCAGTGCGG AGCAACAACC AAAGAAGACC
    ATGGCCGCTG TTGTGATGGA AAATTTGGCC AAATTTGATC AACATTTTAC GCTATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17975
    Clone ID Related Accession (Same CDS sequence) NM_001144452.2
    Accession Version NM_001144452.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3330bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smaug, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001144452.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGTACG CAACTGGAAC TGACAACGCG ATGACCTCCG GCATCAGCGG CCAAACCAAT 
    AACTCTAACA GTGCATCCAA CGAGATGCAG CCAACCACAT CAACGCCTAC TGCAGCCCAC
    AAGGAGGCCA CATCAACGGC CACGACGACG GCGACATACG CCAATGGCAA TCCCAATTCT
    AACGCCAATC CCAGCCAGAG CCAGCCGTCC AATGCGCTGT TTTGCGAGCA GGTGACCACA
    GTGACAAATC TCTTCGAGAA GTGGAACGAC TGCGAGCGAA CCGTCGTGAT GTACGCGCTG
    CTCAAGCGGC TGCGCTACCC GAGCCTCAAG TTTCTACAGT ATTCTATCGA CAGCAACCTG
    ACCCAGAACC TGGGCACCTC CCAGACCAAT CTGAGCAGCG TGGTTATTGA CATCAATGCC
    AACAATCCGG TGTACCTGCA GAATCTACTC AACGCCTACA AGACGTTCCA GCCATGTGAC
    CTGCTCGACG CCATGTCCTC CTCGTCGTCC GACAAGGACT CGATGCCATG CTACGGCAGT
    GACTTCCAGA TCACCACATC GGCGCAGTGT GACGAGCGGA AGCTGTATGC CCGCAAGGAG
    GACATTCTGC ATGAAGTACT GAACATGCTG CCTCTGCTGA AGCCGGGCAA TGAGGAGGCC
    AAGCTTATCT ACCTGACCCT TATACCAGTT GCCGTCAAGG ACACCATGCA GCAAATTGTG
    CCCACGGAGT TGGTGCAGCA GATCTTCTCG TACCTACTCA TCCATCCAGC TATCACCAGC
    GAGGACAGAC GTTCGCTCAA CATTTGGCTG CGTCACTTGG AGGATCATAT CCAAGCGGCT
    GCGGCGGGCC TGACAAATCG CAGTTACTTC CTGCAGCCCT CGCCGCAACT GGTCGCTGGT
    GGTAGCTCAA CAGGCAGTGG TAGCTGTTCC TCTTCGGCGA CCAGCTCTTC GACAGCATCC
    TGTTCGTCGG TGGCCTCATC CTCGTTGTGC CCCGCAAGCG GCAGCCGTTC CTCACGCACC
    AACGACTGGC AAACGATCGC CCCGCCCAGC AAGCAATTGC AAAACAAATT GGCCGGTGAT
    TGGCGTGGCA ATGGAGGCGG CAGCTCCAGC GGGTCGATCA ATCCACTCTG TGATAATCTT
    AATGGTATTA CCCTGAACGA ATTAGCTTCT AGTCAGAACA GTCTCGGCCT GTCGCTGGAG
    GGCAGCTCTT CCCTGGTCAA CGGTGTGGTC GCTGGTGCAG GCTCTATGTT GGGAATCGCT
    GGTGGCGACG ATCATGACAC GTCATTTAGC AAGAATGGCA CGGAGATTCT CGATTTTGAC
    CCGGTGACGG CGGACATGGG TGAGGCCTGT AGTCTGGCCA GCAGTAGCCT TTGTGGACGT
    AACGGTGGAA ATCCTGTTGA GGATCGATCG CAACCTCCGC CGAATCTTCA GCAGCAATTG
    CTTCAGCCTC CTCCCTATGC CTCGATCCTT ATGGGAAACG TGGGCGATCA GTTTGGAGAG
    ATCAACCGGT GGAGCCTGGA CAGCAAGATT GCAGCCCTGA AGACGCGGCG CTCTAACAGC
    CTCACCACGC AAACGATCTC TAGCTGTTCC AGCTCGTCTA ACTCATCGGT GATCACGGTC
    AATGACAACT GCTCCAATTC AACGGAGAAT CTGGCACAGT TTGCCAACAA GCCCCGTAGT
    TTTTCCTTAT CGATTGAGCA TCAGCGCGGC GCCTTGATGA ACAGCGGTTC CGATACTCGT
    CTGGATGAAT TCAAGCCCAA TTATATTAAG TTCCACACGC GCAACGTGGG CATGTCGGGA
    ATCGGACTCT GGCTAAAATC CTTGCGTCTG CACAAGTACA TCGAGCTGTT CAAGAACATG
    ACGTACGAGG AAATGCTCTT GATCACCGAG GACTTTCTGC AGAGTGTTGG CGTCACCAAG
    GGCGCCTCCC ACAAGCTTGC CCTTTGCATT GATAAGCTGA AGGAACGGGC CAATATTCTT
    AACCGGGTAG AACAAGAACT GCTATCGGGT CAAATGGAGC TGAGCACGGC GGTTGAGGAG
    CTAACAAATA TTGTGCTGAC ACCAATGAAG CCACTGGAGT CTCCAGGACC ACCGGAGGAA
    AACATTGGTC TGCGTTTTCT TAAGGTCATT GACATTGTGA CCAATACGTT GCAGCAGGAT
    CCGTATGCTG TCCAAGACGA CGAGACTTTG GGTGTGCTTA TGTGGATTCT AGACCGCTCT
    ATCCACAACG AGGCATTTAT GAATCACGCC AGTCAACTCA AGGACCTGAA GTTTAAGTTG
    TCCAAAATGA AGATCTCCAT GGTTCCCAAA ATGCATCACG TCAAGCCAGC CGGCGTCGGG
    CCAAACAATG GGAACATTAA CAAGCCAAGA TGGAATGGTA AGACTCGCAA ATGCGATACT
    AAAAATGGCA GCAACGATAG GATTAACAAC CGCAAAAACT CCAACGACAT GCTAAACTTT
    TCGCTAAATT GCCTGCCACA CCCGCTGCCC CATCATTCGC AGCAGGCTCC GCCGCCTCTG
    CCACAGTTTG ACTACAACGG CTATGGCGGC GGTCCATCCC ATCAGCCGCA GTACAAGAGC
    TCCTCGTACC CAAGCTTCAT GGGTAATCCT CAGCAGCAGC CACCACCGCC GCCGTCTAGT
    AAGTCGCATC ATCATCCCCA GCAGATGCAA CAGATGTTGC AGCAACACAA CCACTTTCCA
    GCGCTGCCTC AGCAGACGCC ACCACAATCG CATCGCCGGT CGCTGAATAA TCTTATTCTG
    GTGGCCGGAG GTCCGCAGCA ACCGCAGCAA TTGATCTTTA AGCCCGGTCA GGGAGTGCTC
    ACCAACAACG GATCTAACGA CAACCTGGTA TTGGAACGCA ATCAGCAGTC GCAGCAGCAG
    CAGCAGCAGA GAAAACTTAG CGGAGGAGTG TCCAGTGCGG AGCAACAACC AAAGAAGACC
    ATGGCCGCTG TTGTGATGGT TAGTAATCCA AACCAGAATC AGGACCAGCA CGATCAGCCC
    CAGATCCTGA TCAACAACAA TAATAACAAC AGCATGCTGA ACAACAATTT GATTAACCAG
    CAACAGTTGC AACTTTTGGC AGCAGCTGCT GCCGCCGTGG GCAGTGGCAG CTGTCTGTGC
    TCCAATGGTG GTGGTGGTGC CTGTGTCCAC AATATCTGTC ACCAAAGCAG CAAGAACAAC
    AATCATGGCG TAGACCATTG CCTGTCCCAG TCACCGTTGG GCAGCCTGGG CATGTCGCCC
    CACGTCACCG AGTACAAGAT GAACGACTTT AAGAGCCTAG AACAGCTGGA GACGTTGTGT
    CGCCAAATGA CTGAACAGGC AATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137924.1
    CDS340..3669
    Translation

    Target ORF information:

    RefSeq Version NM_001144452.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smaug (smg), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144452.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGTACG CAACTGGAAC TGACAACGCG ATGACCTCCG GCATCAGCGG CCAAACCAAT 
    AACTCTAACA GTGCATCCAA CGAGATGCAG CCAACCACAT CAACGCCTAC TGCAGCCCAC
    AAGGAGGCCA CATCAACGGC CACGACGACG GCGACATACG CCAATGGCAA TCCCAATTCT
    AACGCCAATC CCAGCCAGAG CCAGCCGTCC AATGCGCTGT TTTGCGAGCA GGTGACCACA
    GTGACAAATC TCTTCGAGAA GTGGAACGAC TGCGAGCGAA CCGTCGTGAT GTACGCGCTG
    CTCAAGCGGC TGCGCTACCC GAGCCTCAAG TTTCTACAGT ATTCTATCGA CAGCAACCTG
    ACCCAGAACC TGGGCACCTC CCAGACCAAT CTGAGCAGCG TGGTTATTGA CATCAATGCC
    AACAATCCGG TGTACCTGCA GAATCTACTC AACGCCTACA AGACGTTCCA GCCATGTGAC
    CTGCTCGACG CCATGTCCTC CTCGTCGTCC GACAAGGACT CGATGCCATG CTACGGCAGT
    GACTTCCAGA TCACCACATC GGCGCAGTGT GACGAGCGGA AGCTGTATGC CCGCAAGGAG
    GACATTCTGC ATGAAGTACT GAACATGCTG CCTCTGCTGA AGCCGGGCAA TGAGGAGGCC
    AAGCTTATCT ACCTGACCCT TATACCAGTT GCCGTCAAGG ACACCATGCA GCAAATTGTG
    CCCACGGAGT TGGTGCAGCA GATCTTCTCG TACCTACTCA TCCATCCAGC TATCACCAGC
    GAGGACAGAC GTTCGCTCAA CATTTGGCTG CGTCACTTGG AGGATCATAT CCAAGCGGCT
    GCGGCGGGCC TGACAAATCG CAGTTACTTC CTGCAGCCCT CGCCGCAACT GGTCGCTGGT
    GGTAGCTCAA CAGGCAGTGG TAGCTGTTCC TCTTCGGCGA CCAGCTCTTC GACAGCATCC
    TGTTCGTCGG TGGCCTCATC CTCGTTGTGC CCCGCAAGCG GCAGCCGTTC CTCACGCACC
    AACGACTGGC AAACGATCGC CCCGCCCAGC AAGCAATTGC AAAACAAATT GGCCGGTGAT
    TGGCGTGGCA ATGGAGGCGG CAGCTCCAGC GGGTCGATCA ATCCACTCTG TGATAATCTT
    AATGGTATTA CCCTGAACGA ATTAGCTTCT AGTCAGAACA GTCTCGGCCT GTCGCTGGAG
    GGCAGCTCTT CCCTGGTCAA CGGTGTGGTC GCTGGTGCAG GCTCTATGTT GGGAATCGCT
    GGTGGCGACG ATCATGACAC GTCATTTAGC AAGAATGGCA CGGAGATTCT CGATTTTGAC
    CCGGTGACGG CGGACATGGG TGAGGCCTGT AGTCTGGCCA GCAGTAGCCT TTGTGGACGT
    AACGGTGGAA ATCCTGTTGA GGATCGATCG CAACCTCCGC CGAATCTTCA GCAGCAATTG
    CTTCAGCCTC CTCCCTATGC CTCGATCCTT ATGGGAAACG TGGGCGATCA GTTTGGAGAG
    ATCAACCGGT GGAGCCTGGA CAGCAAGATT GCAGCCCTGA AGACGCGGCG CTCTAACAGC
    CTCACCACGC AAACGATCTC TAGCTGTTCC AGCTCGTCTA ACTCATCGGT GATCACGGTC
    AATGACAACT GCTCCAATTC AACGGAGAAT CTGGCACAGT TTGCCAACAA GCCCCGTAGT
    TTTTCCTTAT CGATTGAGCA TCAGCGCGGC GCCTTGATGA ACAGCGGTTC CGATACTCGT
    CTGGATGAAT TCAAGCCCAA TTATATTAAG TTCCACACGC GCAACGTGGG CATGTCGGGA
    ATCGGACTCT GGCTAAAATC CTTGCGTCTG CACAAGTACA TCGAGCTGTT CAAGAACATG
    ACGTACGAGG AAATGCTCTT GATCACCGAG GACTTTCTGC AGAGTGTTGG CGTCACCAAG
    GGCGCCTCCC ACAAGCTTGC CCTTTGCATT GATAAGCTGA AGGAACGGGC CAATATTCTT
    AACCGGGTAG AACAAGAACT GCTATCGGGT CAAATGGAGC TGAGCACGGC GGTTGAGGAG
    CTAACAAATA TTGTGCTGAC ACCAATGAAG CCACTGGAGT CTCCAGGACC ACCGGAGGAA
    AACATTGGTC TGCGTTTTCT TAAGGTCATT GACATTGTGA CCAATACGTT GCAGCAGGAT
    CCGTATGCTG TCCAAGACGA CGAGACTTTG GGTGTGCTTA TGTGGATTCT AGACCGCTCT
    ATCCACAACG AGGCATTTAT GAATCACGCC AGTCAACTCA AGGACCTGAA GTTTAAGTTG
    TCCAAAATGA AGATCTCCAT GGTTCCCAAA ATGCATCACG TCAAGCCAGC CGGCGTCGGG
    CCAAACAATG GGAACATTAA CAAGCCAAGA TGGAATGGTA AGACTCGCAA ATGCGATACT
    AAAAATGGCA GCAACGATAG GATTAACAAC CGCAAAAACT CCAACGACAT GCTAAACTTT
    TCGCTAAATT GCCTGCCACA CCCGCTGCCC CATCATTCGC AGCAGGCTCC GCCGCCTCTG
    CCACAGTTTG ACTACAACGG CTATGGCGGC GGTCCATCCC ATCAGCCGCA GTACAAGAGC
    TCCTCGTACC CAAGCTTCAT GGGTAATCCT CAGCAGCAGC CACCACCGCC GCCGTCTAGT
    AAGTCGCATC ATCATCCCCA GCAGATGCAA CAGATGTTGC AGCAACACAA CCACTTTCCA
    GCGCTGCCTC AGCAGACGCC ACCACAATCG CATCGCCGGT CGCTGAATAA TCTTATTCTG
    GTGGCCGGAG GTCCGCAGCA ACCGCAGCAA TTGATCTTTA AGCCCGGTCA GGGAGTGCTC
    ACCAACAACG GATCTAACGA CAACCTGGTA TTGGAACGCA ATCAGCAGTC GCAGCAGCAG
    CAGCAGCAGA GAAAACTTAG CGGAGGAGTG TCCAGTGCGG AGCAACAACC AAAGAAGACC
    ATGGCCGCTG TTGTGATGGT TAGTAATCCA AACCAGAATC AGGACCAGCA CGATCAGCCC
    CAGATCCTGA TCAACAACAA TAATAACAAC AGCATGCTGA ACAACAATTT GATTAACCAG
    CAACAGTTGC AACTTTTGGC AGCAGCTGCT GCCGCCGTGG GCAGTGGCAG CTGTCTGTGC
    TCCAATGGTG GTGGTGGTGC CTGTGTCCAC AATATCTGTC ACCAAAGCAG CAAGAACAAC
    AATCATGGCG TAGACCATTG CCTGTCCCAG TCACCGTTGG GCAGCCTGGG CATGTCGCCC
    CACGTCACCG AGTACAAGAT GAACGACTTT AAGAGCCTAG AACAGCTGGA GACGTTGTGT
    CGCCAAATGA CTGAACAGGC AATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.